dvi_13855

exon [dvir_GLEANR_3494:2]; CDS [Dvir\GJ18667-cds]; CDS [Dvir\GJ18667-cds]; exon [dvir_GLEANR_3494:3]; intron [Dvir\GJ18667-in]; Antisense to intron [Dvir\GJ19479-in]

Sense Strand Reads

Anti-sense strand reads

##################################################--------------------------------------------------------################################################## AGAGTCAAGCGAAAATTGCTGAACTGGAAGGCTACGTAGCACACTTGCAGGTAAGAATAACGCTTCTTGCTATCAGGGATAGGAATACTCGTGGATTGCCTTTCAGGATGAGAAGCCTCTAGAAGCGCTCTTCGCCAAAGATCTGAAGCGTATGGA ************************************************...................((...(((..((.((....)).)).....)))...))************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	SRR060678 9x140_testes_total	SRR060689 160x9_testes_total	V047 embryo	SRR060662 9x160_0-2h_embryos_total	SRR060674 9x140_ovaries_total	SRR060661 160x9_0-2h_embryos_total	SRR060663 160_0-2h_embryos_total	SRR060664 9_males_carcasses_total	SRR060657 140_testes_total
...........AAAATTGCTGAACTGGACGG.............................................................................................................................	20	1	1	1.00	1	0	1	0	0	0	0	0	0	0
.....................AACTGGAAGGCTACGTAGCAC..................................................................................................................	21	0	1	1.00	1	1	0	0	0	0	0	0	0	0
.................................GCGTAGCACACTGGCAGG.........................................................................................................	18	2	7	0.14	1	0	0	1	0	0	0	0	0	0
............................................................CACTTCTTTCGATCAGGGA.............................................................................	19	3	8	0.13	1	0	0	0	0	1	0	0	0	0
............AAATTGTTGAAATGGAAGG.............................................................................................................................	19	2	8	0.13	1	0	0	0	1	0	0	0	0	0
..................CGGAGCTGGAAGGCTAAGT.......................................................................................................................	19	3	10	0.10	1	0	0	0	0	0	0	1	0	0
.................................................GGTAAGAAGAACGGGTCTT........................................................................................	19	3	10	0.10	1	0	0	0	0	0	1	0	0	0
...........................................................................................................................CCCGCTCTTCGTCAAAGAT..............	19	3	15	0.07	1	0	0	0	0	0	0	0	1	0
...........AAAATTCCTGAACCGGGA...............................................................................................................................	18	3	20	0.05	1	0	0	0	0	0	0	0	0	1

TCTCAGTTCGCTTTTAACGACTTGACCTTCCGATGCATCGTGTGAACGTCCATTCTTATTGCGAAGAACGATAGTCCCTATCCTTATGAGCACCTAACGGAAAGTCCTACTCTTCGGAGATCTTCGCGAGAAGCGGTTTCTAGACTTCGCATACCT ************************************************...................((...(((..((.((....)).)).....)))...))************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	SRR060670 9_testes_total	SRR060682 9x140_0-2h_embryos_total	M061 embryo	SRR060686 Argx9_0-2h_embryos_total	GSM1528803 follicle cells
...............................GATGCTCGGTGTGAACGTCCAT.......................................................................................................	22	3	2	0.50	1	1	0	0	0	0
................................................................................TCCTTATGAGCCCGTATCG.........................................................	19	3	7	0.43	3	0	2	0	1	0
...................................................................................................................................AGCGGTTTCTAGGGTTCGCTT....	21	3	7	0.14	1	0	0	1	0	0
...........................................................................CCCGATCCTTATGTGCAAC..............................................................	19	3	13	0.08	1	0	0	0	0	1

Re-alignment of all predicted orthologs

Species	Coordinate	ID	Alignment
droVir3	scaffold_12970:9995541-9995696 -	dvi_13855	AGAGTCAAGCGAAAATTGCTGAACTGGAAGGCTACGTAGCACACTTGCAGGTAAGAATAACGCTTCTTGCTATCAGGGATAGGAATACTCGTGGAT---------------------------TGCCTTTCAGG------------ATGAGAAGCCTCTAGAAGCGCTCTTCGCCAAAGATCTGAAGCGTATGGA
droMoj3	scaffold_6496:13670155-13670219 -		AAAGCCAAGCCCTCATTGAACAAATGCAACAGTACATTCAACAGCTGGAGGTAAGCATCATGATT----------------------------------------------------------------------------------------------------------------------------------
droGri2	scaffold_15112:3924125-3924177 +		AGAGCCATGCGATGATTGAGCAAATGCAGCAATATATACACCAGCTGGAGGTA----------------------------------------------------------------------------------------------------------------------------------------------
droWil2	scf2_1100000004382:918265-918370 +		AGAGTCAGGCTTTGATTGAGCAACTGCAGCAACACATACGCGACCACCAACAATC----------------------A--TCGGACACCAGTGCATCCCCAAT----------------------------GACCTG-------------------------------------TCGGAGATTTAAACGCATGGA
dp5	3:8171685-8171710 +		AAAGCAATGCGTTGATCGATGAGCTG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droPer2	scaffold_4:2049751-2049924 +		AAAGCAATGCGTTGATCGATGAGCTGCAGCAGCATCTGCGCCACTACGAGGTGAGTATCTTGTATG--TCT-TGAGCAGTCATGACCTCTATTCCCTACTTGTGCATTTTAGGAAACTGGAGCTGCCTTTGAAGGAGAGTCCAGAGAA---------------GCAC---TCGATGAAAAGCTCAAACACTTGGA
droAna3	scaffold_13266:16634944-16635112 -		AAAGTCAGGCCATGATTGATCATCTGCAGGAACAAATACGCGGCTATCAGGTGAGATAAA--------CAT-AAACTATTAAAGATTAAAGTTCATTCCTTATTCCTTCTGA-----------------ACAGGCTGCATCTAGAGCTGGCAACCCAAGTGCTGTGATTGACGCTGAGAAGCTCAGGCGGCTGGA
droBip1	scf7180000396384:744018-744184 -		AGAGTCAGACCATGATCGAGCATCTGCAGCAACAAATACGCGGCTATGAGGTGGGATTACAGCATATACAT-ACACCATTTCGGACTAAAATGTAA---------------------------TCCCTTACAGGCTGCGGCCAGAGCTGTCCACCCAAGTGCTGCGATCGACGCTGAGAAGCTCATACGTTTGGA
droKik1	scf7180000302277:506054-506108 +		ACAGTCAGACCATAATAGATGGACTGCAGGAACAGATACGCGCATATGAAGTAAG--------------------------------------------------------------------------------------------------------------------------------------------
droFic1	scf7180000454039:140588-140617 -		AAAGCCAGATGTTAATCGAAGAGTTGCAGG---------------------------------------------------------------------------------------------------------------------------------------------------------------------
droEle1	scf7180000491282:562766-562791 -		ACAGCCAGGCGATAATTGATGATCTG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droRho1	scf7180000759836:33163-33192 +		ACAGCCAGGCGATAATTGATGACTTGCAGG---------------------------------------------------------------------------------------------------------------------------------------------------------------------
droBia1	scf7180000302291:842501-842530 +		ACAGCCAGACAATTATCGATGAGTTGCAGG---------------------------------------------------------------------------------------------------------------------------------------------------------------------
droTak1	scf7180000415379:502704-502733 -		ACAGCCAGAGAATAATCGATGAGTTGCAGG---------------------------------------------------------------------------------------------------------------------------------------------------------------------
droEug1	scf7180000409758:395597-395626 +		AAAGTCAGACTATTATTGATGAGTTGCAGG---------------------------------------------------------------------------------------------------------------------------------------------------------------------
droSim2	2r:9995272-9995326 -		AAAGCCAGGCAATAATCGACGAGTTGCAAGAGCAAATAAACGCCTATCAGGTGAG--------------------------------------------------------------------------------------------------------------------------------------------
droSec2	scaffold_1:6851394-6851448 -		AAAGCCAGGCAATAATCGACGAGTTGCAAGAGCAAATAAACGCCTATCAGGTGAG--------------------------------------------------------------------------------------------------------------------------------------------
droYak3	2R:7626539-7626593 +		ACAGCCAGGCAATGATCGACGAGTTGCAAGAGCAGATACACGCCTATCAGGTGAG--------------------------------------------------------------------------------------------------------------------------------------------
droEre2	scaffold_4845:16485029-16485058 +		ACAGCCAGGCATTAATCGATGAGTTGCAAG---------------------------------------------------------------------------------------------------------------------------------------------------------------------

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droVir3

AGAGTCAAGCGAAAATTGCTGAACTGGAAGGCTACGTAGCACACTTGCAGGTAAGAATAACGCTTCTTGCTATCAGGGATAGGAATACTCGTGGAT---------------------------TGCCTTTCAGG------------ATGAGAAGCCTCTAGAAGCGCTCTTCGCCAAAGATCTGAAGCGTATGGA	Size	Hit Count	Total Norm	Total	M027 Male-body	M028 Head	M047 Female-body	M061 Embryo	SRR060654 Ovary	SRR060655 Testis	SRR060656 Ovary	SRR060657 Testis	SRR060658 Ovary	SRR060659 Testis	SRR060660 Ovary	SRR060661 Embryo	SRR060662 Embryo	SRR060663 Embryo	SRR060664 Male-body	SRR060665 Female-body	SRR060666 Male-body	SRR060667 Female-body	SRR060668 Male-body	SRR060669 Female-body	SRR060670 Testis	SRR060671 Male-body	SRR060672 Female-body	SRR060673 Ovary	SRR060674 Ovary	SRR060675 Ovary	SRR060676 Ovary	SRR060677 Ovary	SRR060678 Testis	SRR060679 Testis	SRR060680 Testis	SRR060681 Testis	SRR060682 Embryo	SRR060683 Testis	SRR060684 Embryo	SRR060685 Embryo	SRR060686 Embryo	SRR060687 Embryo	SRR060688 Ovary	SRR060689 Testis	SRR1106712 embryo_0-2h	SRR1106713 embryo_0-2h	SRR1106714 embryo_2-4h	SRR1106715 embryo_4-6h	SRR1106716 embryo_4-6h	SRR1106717 embryo_6-8h	SRR1106718 embryo_6-8h	SRR1106719 embryo_8-10h	SRR1106720 embryo_8-10h	SRR1106721 embryo_10-12h	SRR1106722 embryo_10-12h	SRR1106723 embryo_12-14h	SRR1106724 embryo_12-14h	SRR1106725 embryo_14-16h	SRR1106726 embryo_14-16h	SRR1106727 larvae	SRR1106728 larvae	SRR1106729 mixed whole adult body	SRR1106730 embryo_16-30h	V047 Embryo	V053 Head	V116 Male-body	GSM1528803 follicle cells
.....................AACTGGAAGGCTACGTAGCAC.........................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

droMoj3

AAAGCCAAGCCCTCATTGAACAAATGCAACAGTACATTCAACAGCTGGAGGTAAGCATCATGATT----------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M046 Female-body	V041 Embryo	V049 Head	V056 Head	M060 Embryo	V110 Male-body
No Reads

droGri2

AGAGCCATGCGATGATTGAGCAAATGCAGCAATATATACACCAGCTGGAGGTA----------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M041 Female-body	V109 Male-body
No Reads

droWil2

AGAGTCAGGCTTTGATTGAGCAACTGCAGCAACACATACGCGACCACCAACAATC----------------------A--TCGGACACCAGTGCATCCCCAAT----------------------------GACCTG-------------------------------------TCGGAGATTTAAACGCATGGA	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
No Reads

dp5

AAAGCAATGCGTTGATCGATGAGCTG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------

Size

Hit Count

Total Norm

Total

M022
Male-body

M040
Female-body

M059
Embryo

M062
Head

V043
Embryo

V051
Head

V112
Male-body

SRR902010
Ovary

SRR902011
Testis

SRR902012
CNS imaginal disc

No Reads

droPer2

AAAGCAATGCGTTGATCGATGAGCTGCAGCAGCATCTGCGCCACTACGAGGTGAGTATCTTGTATG--TCT-TGAGCAGTCATGACCTCTATTCCCTACTTGTGCATTTTAGGAAACTGGAGCTGCCTTTGAAGGAGAGTCCAGAGAA---------------GCAC---TCGATGAAAAGCTCAAACACTTGGA	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
.................................................GGTGAGTATCTTGTATG--TCT-...........................................................................................................................	20	1	1.00	1	0	0	0	0	0	1

droAna3

AAAGTCAGGCCATGATTGATCATCTGCAGGAACAAATACGCGGCTATCAGGTGAGATAAA--------CAT-AAACTATTAAAGATTAAAGTTCATTCCTTATTCCTTCTGA-----------------ACAGGCTGCATCTAGAGCTGGCAACCCAAGTGCTGTGATTGACGCTGAGAAGCTCAGGCGGCTGGA	Size	Hit Count	Total Norm	Total	M044 Female-body	M058 Embryo	V039 Embryo	V055 Head	V105 Male-body	V106 Head
............................................................................................................................................................................GCTGAGAAGCTCAGGCGGCTG..	21	1	1.00	1	1	0	0	0	0	0
.....................................................................................................................................GCTGCATCTAGAGCTGGCAACCC.......................................	23	1	1.00	1	1	0	0	0	0	0
....................................................................................................................................................GGCAACCCAAGTGCTGTGA............................	19	1	1.00	1	1	0	0	0	0	0
..........CATGATTGATCATCTGCAGGAAC..................................................................................................................................................................	23	1	1.00	1	0	1	0	0	0	0
............................GGAACAAATACGCGGCTATCAG.................................................................................................................................................	22	1	1.00	1	1	0	0	0	0	0

droBip1

AGAGTCAGACCATGATCGAGCATCTGCAGCAACAAATACGCGGCTATGAGGTGGGATTACAGCATATACAT-ACACCATTTCGGACTAAAATGTAA---------------------------TCCCTTACAGGCTGCGGCCAGAGCTGTCCACCCAAGTGCTGCGATCGACGCTGAGAAGCTCATACGTTTGGA	Size	Hit Count	Total Norm	Total
No Reads

droKik1

ACAGTCAGACCATAATAGATGGACTGCAGGAACAGATACGCGCATATGAAGTAAG--------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droFic1

AAAGCCAGATGTTAATCGAAGAGTTGCAGG---------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droEle1

ACAGCCAGGCGATAATTGATGATCTG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droRho1

ACAGCCAGGCGATAATTGATGACTTGCAGG---------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droBia1

ACAGCCAGACAATTATCGATGAGTTGCAGG---------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droTak1

ACAGCCAGAGAATAATCGATGAGTTGCAGG---------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droEug1

AAAGTCAGACTATTATTGATGAGTTGCAGG---------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droSim2

AAAGCCAGGCAATAATCGACGAGTTGCAAGAGCAAATAAACGCCTATCAGGTGAG--------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M023 Head	M024 Male-body	M025 Embryo	M053 Female-body	O001 Testis	O002 Head	SRR553485 Ovary	SRR553486 Ovary	SRR553487 Ovary	SRR553488 Ovary	SRR618934 Ovary	V044 Embryo	SRR902008 Ovary	SRR902009 Testis	SRR1275487 Male larvae	SRR1275485 Male prepupae	SRR1275483 Male prepupae
.....CAGGCAATAATCGACGAGTTGCAAGAGCAAA...............................................................................................................................................................	31	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0
.................GACGAGTTGCAAGAGCA.................................................................................................................................................................	17	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0
..................ACGAGTTGCAAGAGC..................................................................................................................................................................	15	1	1.00	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0

droSec2

AAAGCCAGGCAATAATCGACGAGTTGCAAGAGCAAATAAACGCCTATCAGGTGAG--------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M054 Female-body	V113 Male-body	V114 Embryo	V115 Head
...GCCAGGCAATAATCGACG..............................................................................................................................................................................	18	1	1.00	1	0	0	1	0
......AGGCAATAATCGACGAGTTGCAAGAG...................................................................................................................................................................	26	1	1.00	1	0	0	1	0

droYak3

ACAGCCAGGCAATGATCGACGAGTTGCAAGAGCAGATACACGCCTATCAGGTGAG--------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M026 Head	M043 Female-body	M056 Embryo	V046 Embryo	V052 Head	V058 Head	V120 Male-body	SRR1275488 Male larvae	SRR1275486 Male prepupae	SRR1275484 Male prepupae	GSM1528802 follicle cells
..........AATGATCGACGAGTTGCAAGAGCAGATACA...........................................................................................................................................................	30	1	2.00	2	0	0	0	0	0	0	0	0	0	0	2
.............GATCGACGAGTTGCAAGAGCAGAT..............................................................................................................................................................	24	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
.....CAGGCAATGATCGACGAGTTGC........................................................................................................................................................................	22	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0

droEre2

ACAGCCAGGCATTAATCGATGAGTTGCAAG---------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M055 Female-body	M057 Embryo	V060 Head	V040 Embryo	V107 Male-body	V108 Head	GSM1528801 follicle cells
No Reads

dvi_13855

scaffold_12970:9995591-9995646 -

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads