ID:

dvi_128

 Coordinate:

scaffold_12928:591031-591094 -

 Confidence:

confident

 Class:

Canonical miRNA

 Genomic Locale:

intergenic

 [View on UCSC Genome Browser {Cornell Mirror}]
Legend: mature star mismatch in alignment mismatch in read

Predicted structure

-27.3

Flybase annnotation

intergenic

No Repeatable elements found

Sense Strand Reads

GGCTAGGAATAAGGTGCTGCTAACATTTATAAATGAGTCTTTGCATCAAATGCAAGCACTTTGCAGCACTGGATTTTGAATCGGAAACAACCAGAACTCTGAGTGCCTGCTTCTGAAGCAGAGCAAATCACTGCTTAATTTTATTGTTTAATCTAACACTTTCC

***********************************...((((((.(((...(((.((((((...((..((((.((((......))))...))))..))..)))))).)))...))).)))))).....************************************ 		Read size # Mismatch Hit Count Total Norm Total M047

female body

 M028

head

 SRR060681

Argx9_testes_total

 M027

male body		 SRR060672

9x160_females_carcasses_total

 SRR060679

140x9_testes_total

 M061

embryo

 SRR060678

9x140_testes_total

 SRR2096014

Ovary_Strain_160_oxidized		 SRR2096059

Ovary_Min_164		 SRR2096081

embryo_0-2h from 15to16 day old nondysgenic mothers		 SRR2096079

embryo_0-2h from 15to16 day old dysgenic mothers		 SRR060664

9_males_carcasses_total

 SRR060666

160_males_carcasses_total

 SRR060667

160_females_carcasses_total

 SRR060668

160x9_males_carcasses_total

 SRR1106717

embryo_6-8h

 SRR2096038

embryo_0-2h from 14 day old Strain 9 mothers		 SRR2096072

embryo_0-2h from 15to16 day old dysgenic mothers		 V053

head

 SRR1106730

embryo_16-30h

 SRR060673

9_ovaries_total
..................................................TGCAAGCACTTTGCAGCACTGG............................................................................................ 22 0 1 36.00 36 26 1 5 0 1 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0
..................................................TGCAAGCACTTTGCAGCACTGGA........................................................................................... 23 0 1 31.00 31 18 1 2 0 1 3 0 0 0 0 0 1 1 0 1 0 1 1 1 0 0 0
...........................................................................................CAGAACTCTGAGTGCCTGCTTC................................................... 22 0 1 15.00 15 2 7 1 1 1 0 0 0 0 1 0 0 0 1 0 1 0 0 0 0 0 0
..................................................TGCAAGCACTTTGCAGCACTG............................................................................................. 21 0 1 11.00 11 3 4 1 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
..................................................TGCAAGCACTTTGCAGCACTGGAA.......................................................................................... 24 1 1 4.00 4 2 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0
..................................................TGCAAGCACTTTGCAGCAC............................................................................................... 19 0 1 3.00 3 0 1 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
............................................................................................AGAACTCTGAGTGCCTGCTTC................................................... 21 0 1 3.00 3 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
..................................................TGCAAGCACTTTGCAGCACTGGAT.......................................................................................... 24 0 1 2.00 2 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
..................................................TGCAGGCACTTTGCAGCACTGGA........................................................................................... 23 1 1 2.00 2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
..................................................TGCAGGCACTTTGCAGCACTG............................................................................................. 21 1 1 2.00 2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
..................................................TGCAAGCACTTTGCAGCACTGGAC.......................................................................................... 24 1 1 2.00 2 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
..................................................TGCAAGCACTTTGCAGCACTGGAAA......................................................................................... 25 2 1 1.00 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
.................................................ATGCAAGCACTTTGCAGCACTGG............................................................................................ 23 0 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
...................................................GCAAGCACTTTGCAGCACTGGA........................................................................................... 22 0 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
..................................................TGCAAGCACTTTGCAGCACTGGTT.......................................................................................... 24 1 1 1.00 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
..................................................TGCAAGCACTTTGCAGCACTGGTC.......................................................................................... 24 2 1 1.00 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
..................................................TGCAAGCACTTGGCAGCACTG............................................................................................. 21 1 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
...................................................GCAAGCACTTTGCAGCACTGG............................................................................................ 21 0 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
.................................................CTGCAAGCACTTTGCAGCACTGG............................................................................................ 23 1 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
..................................................TGCAGGCACTTTGCAGCACTGG............................................................................................ 22 1 1 1.00 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
...................................................GCAAGCACTTTGCAGCACTGT............................................................................................ 21 1 2 0.50 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
..................................................TGCAAGCACTTTGCAGCACTGT............................................................................................ 22 1 2 0.50 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
..................................................................CACTGGACTTTGAATC.................................................................................. 16 1 7 0.43 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0
...............................................................CAGCACTGGATTTTTAAC................................................................................... 18 2 7 0.14 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
.GCTTGGAATATGCTGCTGCTAA............................................................................................................................................. 22 3 8 0.13 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
......................................................................GGATTTTGAATTGGAAATA........................................................................... 19 2 10 0.10 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
.................................................................GCACCGGATTTTGATTC.................................................................................. 17 2 18 0.06 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0

Anti-sense strand reads

CCGATCCTTATTCCACGACGATTGTAAATATTTACTCAGAAACGTAGTTTACGTTCGTGAAACGTCGTGACCTAAAACTTAGCCTTTGTTGGTCTTGAGACTCACGGACGAAGACTTCGTCTCGTTTAGTGACGAATTAAAATAACAAATTAGATTGTGAAAGG

************************************...((((((.(((...(((.((((((...((..((((.((((......))))...))))..))..)))))).)))...))).)))))).....*********************************** 		Read size # Mismatch Hit Count Total Norm Total V053

head

 SRR060672

9x160_females_carcasses_total

 V116

male body		 M047

female body

 SRR060666

160_males_carcasses_total

 SRR060668

160x9_males_carcasses_total

 SRR060669

160x9_females_carcasses_total

 V047

embryo

 SRR060664

9_males_carcasses_total

 SRR060667

160_females_carcasses_total

 SRR060689

160x9_testes_total

 SRR060686

Argx9_0-2h_embryos_total

 SRR060676

9xArg_ovaries_total

 SRR060665

9_females_carcasses_total

 SRR060659

Argentina_testes_total

 SRR060673

9_ovaries_total
..........................................................................................GGTCTTGAGACTCACGGACGA..................................................... 21 0 1 14.00 14 5 2 2 0 1 1 2 0 0 0 1 0 0 0 0 0
.........................................................................................TGGTCTTGAGACTCACGGACGA..................................................... 22 0 1 12.00 12 5 1 1 1 1 0 0 1 1 1 0 0 0 0 0 0
............................................................................................GCTTGAGACTCACGGACGA..................................................... 19 1 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
..........................................................................................GGTCTAGAGATTCACGGACGA..................................................... 21 2 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
..........................................................................................GGTCTTGAGACTCACGGCCGA..................................................... 21 1 1 1.00 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
........................................................................................TTGGTCTTGAGGCTCACGGACGA..................................................... 23 1 1 1.00 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
........................................................................................GTGGTCTTGAGACTCACGGACGA..................................................... 23 1 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
................................................................................AGCCTTTGTTGGTCTTGAGACTC............................................................. 23 0 1 1.00 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
..........................................................................................GGTCTTGCGACTCACGGACGA..................................................... 21 1 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
............................................................................................TCTTGAGACTCACGGACGA..................................................... 19 0 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
...............................................................GTCGTGGCCTGAAACTTAGC................................................................................. 20 2 2 0.50 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
....................................................................................................CTGATGGACGAAGACTTC.............................................. 18 2 2 0.50 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
..........................................................................................................................................TAAATAACATATTAGATTGT...... 20 2 3 0.33 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
......................................CAAACGTAGTTTACGTTTGTGG........................................................................................................ 22 3 4 0.25 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
...............................................................................................................AGACTTCGTCACGTTTATT.................................. 19 2 7 0.14 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
................................................................................AGCCCGTGTTGGTCTAGAGA................................................................ 20 3 9 0.11 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
...ATCCTTAGTACACCACGAT.............................................................................................................................................. 19 3 11 0.09 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1

Re-alignment of all predicted orthologs

Species Coordinate ID Type Alignment
droVir3 scaffold_12928:590981-591144 - dvi_128 confident GGCTAGGAATAAGGTGCTGCTAACATTTATAAATGAGTCTTTGCATCAAATGCAAGCACTTTGCAGCACTGGATTTTGAATCGGAAACAACCAGAACTCTGAGTGCCTGCTTCTGAAGCAGAGCAAATCACTGCTTAATTTTAT---TGTTTAATC------TAACACTTTCC
droMoj3 scaffold_6680:17147976-17148005 - TCA--------------------------------------------------------------------------------------------------------------------------------------ATTTTAC---AGGACCATC------CCAAATATTCC
droGri2 scaffold_8094:777-806 + ATTT-----------------------------------------------------------------------------------------------------------------------------------------TATGTACGGTTAGTT------TTACATCTCTT
dp5 XL_group1a:4484982-4484997 - ATTT-------------------------------------------------------------------------------------------------------------------------------------------------------T------TTAAATTTCCT
droPer2 scaffold_11:1069844-1069856 - T----------------------------------------------------------------------------------------------------------------------------------------------------------T------TAAAATTTCCT
droEle1 scf7180000490214:1425294-1425344 + AGG-----------------------------------------------------------------------------------------------------------------------GCGTGTTACTGCATCAATTTAT---GGTTGAATTTTGGTGTCCACTCCTTC

Re-alignment of all predicted orthologs with sRNA reads

Species Read alignment
droVir3
GGCTAGGAATAAGGTGCTGCTAACATTTATAAATGAGTCTTTGCATCAAATGCAAGCACTTTGCAGCACTGGATTTTGAATCGGAAACAACCAGAACTCTGAGTGCCTGCTTCTGAAGCAGAGCAAATCACTGCTTAATTTTAT---TGTTTAATC------TAACACTTTCCSizeHit CountTotal NormTotalM027

Male-body		M028

Head		M047

Female-body		M061

Embryo		SRR060654

Ovary		SRR060655

Testis		SRR060656

Ovary		SRR060657

Testis		SRR060658

Ovary		SRR060659

Testis		SRR060660

Ovary		SRR060661

Embryo		SRR060662

Embryo		SRR060663

Embryo		SRR060664

Male-body		SRR060665

Female-body		SRR060666

Male-body		SRR060667

Female-body		SRR060668

Male-body		SRR060669

Female-body		SRR060670

Testis		SRR060671

Male-body		SRR060672

Female-body		SRR060673

Ovary		SRR060674

Ovary		SRR060675

Ovary		SRR060676

Ovary		SRR060677

Ovary		SRR060678

Testis		SRR060679

Testis		SRR060680

Testis		SRR060681

Testis		SRR060682

Embryo		SRR060683

Testis		SRR060684

Embryo		SRR060685

Embryo		SRR060686

Embryo		SRR060687

Embryo		SRR060688

Ovary		SRR060689

Testis		SRR1106712

embryo_0-2h		SRR1106713

embryo_0-2h		SRR1106714

embryo_2-4h		SRR1106715

embryo_4-6h		SRR1106716

embryo_4-6h		SRR1106717

embryo_6-8h		SRR1106718

embryo_6-8h		SRR1106719

embryo_8-10h		SRR1106720

embryo_8-10h		SRR1106721

embryo_10-12h		SRR1106722

embryo_10-12h		SRR1106723

embryo_12-14h		SRR1106724

embryo_12-14h		SRR1106725

embryo_14-16h		SRR1106726

embryo_14-16h		SRR1106727

larvae		SRR1106728

larvae		SRR1106729

mixed whole adult body		SRR1106730

embryo_16-30h		V047

Embryo		V053

Head		V116

Male-body		GSM1528803

follicle cells		SRR2096013

Ovary_Strain_9_oxidized		SRR2096014

Ovary_Strain_160_oxidized		SRR2096026

embryo_0-2h from 14 day old Strain 9 mothers		SRR2096038

embryo_0-2h from 14 day old Strain 9 mothers		SRR2096050

embryo_0-2h from 14 day old Strain 160 mothers		SRR2096055

Ovary_Min_43		SRR2096056

Ovary_Min_46		SRR2096057

Ovary_Min_50		SRR2096058

Ovary_Min_98		SRR2096059

Ovary_Min_164		SRR2096060

Ovary_Min_193		SRR2096061

embryo_0-2h from 14 day old Strain 160 mothers		SRR2096072

embryo_0-2h from 15to16 day old dysgenic mothers		SRR2096079

embryo_0-2h from 15to16 day old dysgenic mothers		SRR2096080

embryo_0-2h from 15to16 day old nondysgenic mothers		SRR2096081

embryo_0-2h from 15to16 day old nondysgenic mothers
..................................................TGCAAGCACTTTGCAGCACTGG.....................................................................................................22136.003601260000000000000000000100000100500000000000000000000000000000000200000000000000
..................................................TGCAAGCACTTTGCAGCACTGGA....................................................................................................23131.003101180000000000010010000100000030200000000000001000000000000000000001000000001100
...........................................................................................CAGAACTCTGAGTGCCTGCTTC............................................................22115.00151720000000000000101000100000000100000000000000000000000000000000000000001000000
..................................................TGCAAGCACTTTGCAGCACTG......................................................................................................21111.00112430000000000000000000100000000100000000000000000000000000000000000000000000000
..................................................TGCAAGCACTTTGCAGCACTGGAA...................................................................................................2414.0040020000000000000000000000000200000000000000000000000000000000000000000000000000
..................................................TGCAAGCACTTTGCAGCAC........................................................................................................1913.0030101000000000000000000000000000000000000000000000000000000000000000000001000000
............................................................................................AGAACTCTGAGTGCCTGCTTC............................................................2113.0031110000000000000000000000000000000000000000000000000000000000000000000000000000
..................................................TGCAAGCACTTTGCAGCACTGGAT...................................................................................................2412.0020011000000000000000000000000000000000000000000000000000000000000000000000000000
..................................................TGCAAGCACTTTGCAGCACTGGAC...................................................................................................2412.0022000000000000000000000000000000000000000000000000000000000000000000000000000000
..................................................TGCAAGCACTTTGCAGCACTGGAAA..................................................................................................2511.0010000000000000000000000000000000000000000000000000000000000000000000000000000001
.................................................ATGCAAGCACTTTGCAGCACTGG.....................................................................................................2311.0010010000000000000000000000000000000000000000000000000000000000000000000000000000
...................................................GCAAGCACTTTGCAGCACTGGA....................................................................................................2211.0010010000000000000000000000000000000000000000000000000000000000000000000000000000
..................................................TGCAAGCACTTTGCAGCACTGGTC...................................................................................................2411.0010100000000000000000000000000000000000000000000000000000000000000000000000000000
...................................................GCAAGCACTTTGCAGCACTGG.....................................................................................................2111.0010010000000000000000000000000000000000000000000000000000000000000000000000000000
...................................................GCAAGCACTTTGCAGCACTGT.....................................................................................................2120.5010010000000000000000000000000000000000000000000000000000000000000000000000000000
..................................................TGCAAGCACTTTGCAGCACTGT.....................................................................................................2220.5010000000000000000000000000000000000000000000000000000000000001000000000000000000
droMoj3
TCA--------------------------------------------------------------------------------------------------------------------------------------ATTTTAC---AGGACCATC------CCAAATATTCCSizeHit CountTotal NormTotalM046

Female-body		V041

Embryo		V049

Head		V056

Head		M060

Embryo		V110

Male-body
No Reads
droGri2
ATTT-----------------------------------------------------------------------------------------------------------------------------------------TATGTACGGTTAGTT------TTACATCTCTTSizeHit CountTotal NormTotalM041

Female-body		V109

Male-body
No Reads
dp5
ATTT-------------------------------------------------------------------------------------------------------------------------------------------------------T------TTAAATTTCCTSizeHit CountTotal NormTotalM022

Male-body		M040

Female-body		M059

Embryo		M062

Head		V043

Embryo		V051

Head		V112

Male-body		SRR902010

Ovary		SRR902011

Testis		SRR902012

CNS imaginal disc
No Reads
droPer2
T----------------------------------------------------------------------------------------------------------------------------------------------------------T------TAAAATTTCCTSizeHit CountTotal NormTotalM021

Embryo		M042

Female-body		V042

Embryo		V050

Head		V057

Head		V111

Male-body
No Reads
droEle1
AGG-----------------------------------------------------------------------------------------------------------------------GCGTGTTACTGCATCAATTTAT---GGTTGAATTTTGGTGTCCACTCCTTCSizeHit CountTotal NormTotal
No Reads

Generated: 10/20/2015 at 06:58 PM