| GTCCA-------AAA---------------------------------------------CTA-AAC--------------------------TGCATACACTATTTAAAGAAGT-----------TG-----------------------------------------TAATAT------------------------------------------------------------------------------------------------TTTTTAATAATACCCGCTACTCGTA-------GAGTAAAAGTATATACTAGTTTCGTTGAAA-------AGTATGTACCAGGCAGATGGCAGCGTTTTCGACTATA--TAAAGTATATATATTCTTGAT-AGGATCAATAGCCGAGTCGATCTAGCCATGTCCGTCTGCCCGACTGTCCGTCTGTC----C--------GTATGAACGTCGAGATTACAG-----GAA | Size | Hit Count | Total Norm | Total | M023 Head | M024 Male-body | M025 Embryo | M053 Female-body | O001 Testis | O002 Head | SRR553485 Ovary | SRR553486 Ovary | SRR553487 Ovary | SRR553488 Ovary | SRR618934 Ovary | V044 Embryo | SRR902008 Ovary | SRR902009 Testis | SRR1275487 Male larvae | SRR1275485 Male prepupae | SRR1275483 Male prepupae |
| .............................................................................................................................................................................................................................................................................................................................................................................................................................................................TGTCCGTCTGTC----C--------GTATGAACGTCGA................ | 26 | 20 | 15.95 | 319 | 0 | 0 | 206 | 0 | 0 | 0 | 56 | 0 | 57 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..................................................................................................................................................................................................................................................................................................................TAAAAGTATATACTAGTTTCGTTGAA....................................................................................................................................................................... | 26 | 1 | 5.00 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .................................................................................................................................................................................................................................................................................TTTAATAATACCCGCTACTCGTA-------GAGT................................................................................................................................................................................................ | 27 | 1 | 3.00 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .............................................................................................................................................................................................................................................................................................................................................................................................................................CGAGTCGATCTAGCCATGTCCGTC.............................................................. | 24 | 20 | 2.90 | 58 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 55 | 0 | 2 | 0 | 0 | 0 | 0 |
| ........................................................................................................................................................................................................................................................................................................................................TGAAA-------AGTATGTACCAGGCAGATGGC.......................................................................................................................................... | 26 | 1 | 2.00 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...........................................................................................................................................................................................................................................................................................................................................................................................TAAAGTATATATATTCTTGAT-AGGA.............................................................................................. | 25 | 1 | 2.00 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..................................................................................................................................................................................................................................................................................TTAATAATACCCGCTACTCGTA-------GAG................................................................................................................................................................................................. | 25 | 1 | 2.00 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .......................................................................................................................................................................................................................................................................................................A-------GAGTAAAAGTATATAC.................................................................................................................................................................................... | 17 | 1 | 2.00 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..........................................................................................................................................................................................................................................................................................................................................AAA-------AGTATGTACCAGGCAGATGGCAG........................................................................................................................................ | 26 | 1 | 2.00 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..........................................................................................................................................................................................................................................................................................................................................AAA-------AGTATGTACCAGGCAGATGGCA......................................................................................................................................... | 25 | 1 | 2.00 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 |
| ...................................................................................................................................................................................................................................................................................................................................TTCGTTGAAA-------AGTATGTACCAGGC................................................................................................................................................. | 24 | 2 | 2.00 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...CA-------AAA---------------------------------------------CTA-AAC--------------------------TGCATACACTATT......................................................................................................................................................................................................................................................................................................................................................................................................... | 24 | 1 | 2.00 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
| .............................................................................................................................................................................................................................................................................................................................................................................................................................................................TGTCCGTCTGTC----C--------GTATGAACGTCGG................ | 26 | 20 | 1.35 | 27 | 0 | 0 | 27 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...............................................................................................................................................................................................................................................................................................................................................................................................................T-AGGATCAATAGCCGAGTCGATC............................................................................ | 23 | 12 | 1.33 | 16 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 4 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...................................................................................................................................................................................................................................................................................TAATAATACCCGCTACTCGTA-------GAG................................................................................................................................................................................................. | 24 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...................................................................................................................................................................................................................................................................................................................................TTCGTTGAAA-------AGTATGTACCA.................................................................................................................................................... | 21 | 3 | 1.00 | 3 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .........................................................................................................................................................................................................................................................................................TACCCGCTACTCGTA-------GAGTAAAAGTA......................................................................................................................................................................................... | 26 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...............................................................................................................................................................................................................................................................................................................................................................................................................T-AGGATCAATAGCCGAGTCGATCT........................................................................... | 24 | 7 | 1.00 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .............................................................................................................................................................................................................................................................................................................................................................................................................................................TGTCCGTCTGCCCGACTGTCCGTCTG............................................ | 26 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................TCTGTC----C--------GTATGAACGTCGAGATTACAG-----G.. | 29 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .....................................................................................................................................................................................................................................................................................................................................................................................................................................................................TGTC----C--------GTATGAACGTCGAGATTACAG-----GA. | 28 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..........................................................................................................................................................................................................................................................................................................................................AAA-------AGTATGTACCAGGCAGAT............................................................................................................................................. | 21 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
| .......................................................................................................................................................................................................................................................................................................................GTATATACTAGTTTCGTTGAAA-------AG............................................................................................................................................................. | 24 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................TCTGTC----C--------GTATGAACGTCGAGATTA........... | 25 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
| ......................................................................................................................................................................................................................................................................................................................AGTATATACTAGTTTCGTTGAAA-------AGTA........................................................................................................................................................... | 27 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................TC----C--------GTATGAACGTCGAGATTACAG-----GAA | 27 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
| ...................................................................................................................................................................................................................................................................................................................................TTCGTTGAAA-------AGTATGTACCAGGCAGA.............................................................................................................................................. | 27 | 2 | 1.00 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
| .....................................................................................................................................................................................................................................................................................................................................................................TGGCAGCGTTTTCGACTATA--TAAAGTATA............................................................................................................... | 29 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .....................................................................................................................................................................................................................................................................................................................................................................TGGCAGCGTTTTCGACTATA--TAAAGTA................................................................................................................. | 27 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..........................................................................................................................................................................................................................................................................................................................................AAA-------AGTATGTACCAGGCAGATGG........................................................................................................................................... | 23 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ................................................................................................................................................................................................................................................................................................................................................................................................................................GTCGATCTAGCCATGTCCGTCTGCCCGA....................................................... | 28 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .....................................................................................................................................................................................................................................................................................................................................................................................................................................TCTAGCCATGTCCGTCTGCCCGACTGTC.................................................. | 28 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .......................................................................................................................................................................................................................................................................................................................................TTGAAA-------AGTATGTACCAGGCAGATGGC.......................................................................................................................................... | 27 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .............................................................................................................................................................................................................................................................................................................................................................................................AAGTATATATATTCTTGAT-AGGAT............................................................................................. | 24 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................TC----C--------GTATGAACGTCGAGATTACAG-----G.. | 25 | 1 | 1.00 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..........................................................................................................................................................................................................................................................................................................................................AAA-------AGTATGTACCAGGCAGATGGC.......................................................................................................................................... | 24 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................C----C--------GTATGAACGTCGAGATTACAG-----GAA | 26 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
| ......................................................................................................TATTTAAAGAAGT-----------TG-----------------------------------------TAATAT------------------------------------------------------------------------------------------------TTTT................................................................................................................................................................................................................................ | 25 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..........................................................................................................................................................................................................................................................................................................................................AAA-------AGTATGTACCAGGCAGATGGT.......................................................................................................................................... | 24 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ........................................................................................................................................................................................................................................................................................................................................TGAAA-------AGTATGTACCAGGCAGATGGCA......................................................................................................................................... | 27 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................TC----C--------GTATGAACGTCGAGATTACAG-----GA. | 26 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
| ..................................................................................................................................................................................................................................................................................................................TAAAAGTATATACTAGTTTCGTTGAAA-------............................................................................................................................................................... | 27 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..........................................................................................................................................................................................................................................................................................................................................AAA-------AGTATGTACCAGGCAGATGGCAC........................................................................................................................................ | 26 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ......................................................................................................................................................................................................................................................................................................TA-------GAGTAAAAGTATATACTAGTTTCGTT.......................................................................................................................................................................... | 28 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .................................................................................................................................................................................................................................................................................TTTAATAATACCCGCTACTCGTA-------G................................................................................................................................................................................................... | 24 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..........................................................................................................................................................................................................................................................................................................................................AAA-------AGTATGTACCAGGCAGA.............................................................................................................................................. | 20 | 2 | 1.00 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...............................................................................................................................................................................................................................................................................................................................................................................................................T-AGGATCAATAGCCGAGTC................................................................................ | 19 | 15 | 0.93 | 14 | 0 | 0 | 0 | 0 | 0 | 14 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .............................................................................................................................................................................................................................................................................................................................................................................................................................CGAGTCGATCTAGCCATGTCCGTCTGC........................................................... | 27 | 5 | 0.80 | 4 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
| .....................................................................................................................................................................................................................................................................................................................................................................................................................................................................TGTC----C--------GTATGAACGTCGAGATTAA.......... | 24 | 3 | 0.67 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
| ......................................................................................................................................................................................................................................................................................................................................................................................................................CAATAGCCGAGTCGATCTAGCCATGTC.................................................................. | 27 | 20 | 0.65 | 13 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 13 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...............................................................................................................................................................................................................................................................................................................................................................................................................T-AGGATCAATAGCCGAGTCGAT............................................................................. | 22 | 13 | 0.62 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...................................................................................................................................................................................................................................................................................................................................TTCGTTGAAA-------AGTATGTACCAGGCAG............................................................................................................................................... | 26 | 2 | 0.50 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...................................................................................................................................................................................................................................................................................................................................TTCGTTGAAA-------AGTATGTACCAGGCA................................................................................................................................................ | 25 | 2 | 0.50 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..........................................................................................................................................................................................................................................................................................................................................AAA-------AGTATGTACCAGGCAG............................................................................................................................................... | 19 | 2 | 0.50 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
| ......................................................................................................................................................................................................................................................................................................................................................................................................................CAATAGCCGAGTCGATCTAGCCA...................................................................... | 23 | 20 | 0.40 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
| ......................................................................................................................................................................................................................................................................................................TA-------GAGTAAAAGTATA....................................................................................................................................................................................... | 15 | 3 | 0.33 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................GTCTGTC----C--------GTATGAACGTCGAGATT............ | 25 | 3 | 0.33 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ................................................................................................................................................................................................................................................................................................................................................................................................................................................................CCGTCTGTC----C--------GTATGAACGTCGAGATT............ | 27 | 3 | 0.33 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...........................................................................................................................................................................................................................................................................................................................ATACTAGTTTCGTTGAAA-------A.............................................................................................................................................................. | 19 | 3 | 0.33 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...............................................................................................................................................................................................................................................................................................................................................................................................................................................................TCCGTCTGTC----C--------GTATGAACGTCGAGATT............ | 28 | 3 | 0.33 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...............................................................................................................................................................................................................................................................................................................................................................................................................T-AGGATCAATAGCCGAGTCGATCTA.......................................................................... | 25 | 3 | 0.33 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .............................................................................................................................................................................................................................................................................................................................................................................................................................................................TGTCCGTCTGTC----C--------GTATGAACGTCGAGA.............. | 28 | 20 | 0.30 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ......................................................................................................................................................................................................................................................................................................................................................................................................................CAATAGCCGAGTCGATCTAGCCATGT................................................................... | 26 | 20 | 0.30 | 6 | 0 | 0 | 0 | 5 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ................................................................................................................................................................................................................................................................................................................................AGTTTCGTTGAAA-------AGTA........................................................................................................................................................... | 17 | 4 | 0.25 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..........................................................................................................................................................................................................................................................................................................................TATACTAGTTTCGTTG......................................................................................................................................................................... | 16 | 4 | 0.25 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...........................................................................................................................................................................................................................................................................................................................................................................................................................GCCGAGTCGATCTAGCCATGTCCGTC.............................................................. | 26 | 20 | 0.25 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ........................................................................................................................................................................................................................................................................................................................................................................................................................ATAGCCGAGTCGATCTAGCCATGTC.................................................................. | 25 | 20 | 0.25 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
| .............................................................................................................................................................................................................................................................................................................................................................................................................................CGAGTCGATCTAGCCATGTCCGTCT............................................................. | 25 | 20 | 0.20 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 |
| ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................TCTGTC----C--------GTATGAACGTCGAGATT............ | 24 | 5 | 0.20 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .....................................................................................................................................................................................................................................................................................................................................................................................................................................TCTAGCCATGTCCGTCTGCC.......................................................... | 20 | 5 | 0.20 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...........................................................................................................................................................................................................................................................................................................................................................................................................................................................ACTGTCCGTCTGTC----C--------GTATGAACGTC.................. | 26 | 10 | 0.20 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...........................................................................................................................................................................................................................................................................................................................................................................................................................................................ACTGTCCGTCTGTC----C--------GTATGAACGA................... | 25 | 11 | 0.18 | 2 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .....................................................................................................................................................................................................................................................................................................................................................................................................................................TCTAGCCATGTCCGTCTGC........................................................... | 19 | 6 | 0.17 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...........................................................................................................................................................................................................................................................................................................................................................................................................................................................ACTGTCCGTCTGTC----C--------GTATGA....................... | 21 | 12 | 0.17 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ................................................................................................................................................................................................................................................................................................................................................................................................................................GTCGATCTAGCCATGTCCGTCTGC........................................................... | 24 | 6 | 0.17 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ....................................................................................................................................................................................................................................................................................................................................................................................................................................ATCTAGCCATGTCCGTCTGC........................................................... | 20 | 6 | 0.17 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...........................................................................................................................................................................................................................................................................................................................................................................................................................GCCGAGTCGATCTAGCCATGTCCG................................................................ | 24 | 20 | 0.15 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................CTGTC----C--------GTATGAACGTC.................. | 17 | 20 | 0.15 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...............................................................................................................................................................................................................................................................................................................................................................................................................T-AGGATCAATAGCCGAGTCGATCTT.......................................................................... | 25 | 7 | 0.14 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .....................................................................................................................................................................................................................................................................................................................................................................................................................................................................TGTC----C--------GTATGAACGTCGAGATT............ | 22 | 7 | 0.14 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...............................................................................................................................................................................................................................................................................................................................................................................................GTATATATATTCTTGAT-A................................................................................................. | 18 | 14 | 0.14 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .............................................................................................................................................................................................................................................................................................................................................................................................................................................................TGTCCGTCTGTC----C--------GTATGAAC..................... | 21 | 20 | 0.10 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .....................................................................................................................................................................................................................................................................................................................................................................................................................TCAATAGCCGAGTCGATCTAGCCA...................................................................... | 24 | 20 | 0.10 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...........................................................................................................................................................................................................................................................................................................................................................................................................................................................ACTGTCCGTCTGTC----C--------GTATGAACGTCGA................ | 28 | 10 | 0.10 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...........................................................................................................................................................................................................................................................................................................................................................................................................................GCCGAGTCGATCTAGCCATGTCCGTT.............................................................. | 26 | 20 | 0.10 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
| ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................GTCTGTC----C--------GTATGAACGTCGAGA.............. | 23 | 20 | 0.10 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..........................................................................................................................................................................................................................................................................................................................................................................................................................AGCCGAGTCGATCTAGCCATGTCC................................................................. | 24 | 20 | 0.10 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
| .............................................................................................................................................................................................................................................................................................................................................................................................................................................................TGTCCGTCTGTC----C--------GTATGAACG.................... | 22 | 20 | 0.10 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ......................................................................................................................................................................................................................................................................................................................................................................................................................CAATAGCCGAGTCGATCTAGCCATGTCC................................................................. | 28 | 20 | 0.10 | 2 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..................................................................................................................................................................................................................................................................................................................................................................................................................................CGATCTAGCCATGTCCGTCT............................................................. | 20 | 20 | 0.10 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...........................................................................................................................................................................................................................................................................................................................................................................................................................GCCGAGTCGATCTAGCCATGTCC................................................................. | 23 | 20 | 0.10 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .........................................................................................................................................................................................................................................................................................................................................................................................................................TAGCCGAGTCGATCTAGCCATGTCCG................................................................ | 26 | 20 | 0.10 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ............................................................................................................................................................................................................................................................................................................................................................................................................................................................CTGTCCGTCTGTC----C--------GTATG........................ | 19 | 20 | 0.10 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................GTC----C--------GTATGAACGTCGAGATT............ | 21 | 11 | 0.09 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...........................................................................................................................................................................................................................................................................................................................................................................................................................................................ACTGTCCGTCTGTC----C--------GTATGAAC..................... | 23 | 12 | 0.08 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................TC----C--------GTATGAACGTCGAGATT............ | 20 | 13 | 0.08 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ............................................................................................................................................................................................................................................................................................CCGCTACTCGTA-------GAGT................................................................................................................................................................................................ | 16 | 13 | 0.08 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...............................................................................................................................................................................................................................................................................................CTACTCGTA-------GAGTAAA............................................................................................................................................................................................. | 16 | 14 | 0.07 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ............................................................................................................................................................................................................................................................................................................................................................................................................................................................CTGTCCGTCTGTC----C--------GTATGAACGTCGA................ | 27 | 15 | 0.07 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................TCTGTC----C--------GTATGAACGTCGAGA.............. | 22 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...................................................................................................................................................................................................................................................................................................................................................................................................................................GATCTAGCCATGTCCGTCT............................................................. | 19 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ............................................................................................................................................................................................................................................................................................................................................................................................................................................................CTGTCCGTCTGTC----C--------GTATGAACG.................... | 23 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ......................................................................................................................................................................................................................................................................................................................................................................................................................CAATAGCCGAGTCGATCTAGCCATG.................................................................... | 25 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...............................................................................................................................................................................................................................................................................................................................................................................................................................................................TCCGTCTGTC----C--------GTATGAACGTCGA................ | 24 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...................................................................................................................................................................................................................................................................................................................................................................................................................GATCAATAGCCGAGTCGATCTAGC........................................................................ | 24 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ....................................................................................................................................................................................................................................................................................................................................................................................................................ATCAATAGCCGAGTCGATCTAGCCA...................................................................... | 25 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .........................................................................................................................................................................................................................................................................................................................................................................................................................TAGCCGAGTCGATCTAGCCATGTCC................................................................. | 25 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ......................................................................................................................................................................................................................................................................................................................................................................................................................CAATAGCCGAGTCGAT............................................................................. | 16 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ............................................................................................................................................................................................................................................................................................................................................................................................................................CCGAGTCGATCTAGCCATGTCCGTC.............................................................. | 25 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ............................................................................................................................................................................................................................................................................................................................................................................................................................CCGAGTCGATCTAGCCATGTCCGTCT............................................................. | 26 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...........................................................................................................................................................................................................................................................................................................................................................................................................................GCCGAGTCGATCTAGCCATGT................................................................... | 21 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
| .........................................................................................................................................................................................................................................................................................................................................................................................................................TAGCCGAGTCGATCTAGCCAT..................................................................... | 21 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ............................................................................................................................................................................................................................................................................................................................................................................................................................CCGAGTCGATCTAGCCATGTCCGTCTG............................................................ | 27 | 20 | 0.05 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ......................................................................................................................................................................................................................................................................................................................................................................................................................CAATAGCCGAGTCGATCTAGC........................................................................ | 21 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ....................................................................................................................................................................................................................................................................................................................................TCGTTGAAA-------AGTATGTA....................................................................................................................................................... | 17 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...................................................................................................................................................................................................................................................................................................................................................................................................................GATCAATAGCCGAGTCGATCTAGCCAA..................................................................... | 27 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
| .......................................................................................................................................................................................................................................................................................................................................................................................................................AATAGCCGAGTCGATCTAGCCAT..................................................................... | 23 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ............................................................................................................................................................................................................................................................................................................................................................................................................................................................CTGTCCGTCTGTC----C--------GTATGAAC..................... | 22 | 20 | 0.05 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .............................................................................................................................................................................................................................................................................................................................................................................................................................CGAGTCGATCTAGCCATGTCCGT............................................................... | 23 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |