ID:

dsi_97

 Coordinate:

2R:15133515-15133573 -

 Confidence:

Known Ortholog

 Type:

Unknown

 [View on UCSC Genome Browser {Cornell Mirror}]
Legend: mature star mismatch in alignment mismatch in read

Predicted structure

Flybase annnotation

intergenic

No Repeatable elements found

Sense Strand Reads

TAAAAAAGACAAAAATGTGAACTTCAAATTGCCATAATCTCAAAATAGTAAAAAGTCTGTTATGCTAATGTTTGTGTGTTTAACAAACTTGGCATAACCGCTTTTTACTTATTTGCAGAAAACTAACCACAAAGTGCACAGATTGAAAAAAAACACAAG
***********************************..(((((((..((((((((((..((((((((((.((((((.......)))))))))))))))).))))))))))..)))).)))....************************************ 		Read size # Mismatch Hit Count Total Norm Total O001
Testis
 SRR618934
Ovary		 O002
Head
 SRR553488
Ovary		 M024
male body
 SRR553486
Ovary		 SRR553487
Ovary		 GSM343915
embryo
......................................................................................ACTTGGCATAACCGCTTTTTAC................................................... 22 0 1 776.00 776 765 1 10 0 0 0 0 0
......................................................................................ACTTGGCATAACCGCTTT....................................................... 18 0 1 131.00 131 129 0 2 0 0 0 0 0
..................................................AAAAGTCTGTTATGCTAATGTT....................................................................................... 22 0 1 107.00 107 80 19 0 4 0 1 2 1
..................................................AAAAGTCTGTTATGCTAATGTTT...................................................................................... 23 0 1 88.00 88 62 16 2 5 0 2 1 0
.......................................................................................CTTGGCATAACCGCTTTTTAC................................................... 21 0 1 73.00 73 71 0 1 0 1 0 0 0
..................................................AAAAGTCTGTTATGCTAATG......................................................................................... 20 0 1 68.00 68 60 2 1 0 5 0 0 0
..................................................AAAAGTCTGTTATGCTAATGT........................................................................................ 21 0 1 63.00 63 55 6 1 1 0 0 0 0
......................................................................................ACTTGGCATAACCGCTTTTTA.................................................... 21 0 1 61.00 61 61 0 0 0 0 0 0 0
..................................................AAAAGTCTGTTATGCTAA........................................................................................... 18 0 1 57.00 57 57 0 0 0 0 0 0 0
......................................................................................ACTTGGCATAACCGCTTTT...................................................... 19 0 1 49.00 49 46 1 2 0 0 0 0 0
......................................................................................ACTTGGCATAACCGCTTTTT..................................................... 20 0 1 43.00 43 40 3 0 0 0 0 0 0
..................................................AAAAGTCTGTTATGCTAAT.......................................................................................... 19 0 1 34.00 34 33 0 1 0 0 0 0 0
..................................................AAAAGTCTGTTATGCTAATGTTTG..................................................................................... 24 0 1 24.00 24 15 8 1 0 0 0 0 0
.................................................AAAAAGTCTGTTATGCTAATGTTT...................................................................................... 24 0 1 15.00 15 4 7 0 2 1 1 0 0
.......................................................................................CTTGGCATAACCGCTTTT...................................................... 18 0 1 13.00 13 12 0 1 0 0 0 0 0
.................................................AAAAAGTCTGTTATGCTAATG......................................................................................... 21 0 1 13.00 13 9 2 1 0 1 0 0 0
.....................................................................................AACTTGGCATAACCGCTTTTTAC................................................... 23 0 1 12.00 12 12 0 0 0 0 0 0 0
.................................................AAAAAGTCTGTTATGCTAATGTT....................................................................................... 23 0 1 11.00 11 3 8 0 0 0 0 0 0
.................................................AAAAAGTCTGTTATGCTAATGT........................................................................................ 22 0 1 9.00 9 3 6 0 0 0 0 0 0
.......................................................................................CTTGGCATAACCGCTTTTTA.................................................... 20 0 1 8.00 8 8 0 0 0 0 0 0 0
..................................................AAAAGTCTGTTGTGCTAATGTTT...................................................................................... 23 1 1 7.00 7 0 0 0 1 0 0 3 0
.......................................................................................CTTGGCATAACCGCTTTTTACT.................................................. 22 0 1 7.00 7 7 0 0 0 0 0 0 0
.................................................AAAAAGTCTGTTATGCTA............................................................................................ 18 0 1 5.00 5 5 0 0 0 0 0 0 0
.....................................................................................AACTTGGCATAACCGCTT........................................................ 18 0 1 4.00 4 4 0 0 0 0 0 0 0
.....................................................................................AACTTGGCATAACCGCTTTT...................................................... 20 0 1 4.00 4 4 0 0 0 0 0 0 0
......................................................................................ACTTGGCATAACCGCTTTTTACT.................................................. 23 0 1 4.00 4 4 0 0 0 0 0 0 0
.....................................................................................AACTTGGCATAACCGCTTTTT..................................................... 21 0 1 4.00 4 3 1 0 0 0 0 0 0
..................................................AAAAGTCTGTTATGCTAATGTTTGT.................................................................................... 25 0 1 4.00 4 3 0 0 0 0 1 0 0
.......................................................................................CTTGGCATAACCGCTTTTT..................................................... 19 0 1 4.00 4 4 0 0 0 0 0 0 0
...................................................AAAGTCTGTTATGCTAATGTTTG..................................................................................... 23 0 1 3.00 3 3 0 0 0 0 0 0 0
...................................................AAAGTCTGTTATGCTAATGTT....................................................................................... 21 0 1 2.00 2 2 0 0 0 0 0 0 0
.................................................AAAAAGTCTGTTGTGCTAATGTTT...................................................................................... 24 1 1 2.00 2 0 0 0 0 0 0 2 0
......................................................................................ACTTGGCATAACCGCTT........................................................ 17 0 1 2.00 2 0 2 0 0 0 0 0 0
.....................................................................................AACTTGGCATAACCGCTTT....................................................... 19 0 1 2.00 2 2 0 0 0 0 0 0 0
.................................................AAAAAGTCTGTTATGCTAAT.......................................................................................... 20 0 1 2.00 2 2 0 0 0 0 0 0 0
...................................................AAAGTCTGTTATGCTAATG......................................................................................... 19 0 1 2.00 2 2 0 0 0 0 0 0 0
......................................................................................ACTTGGCATAACCGCT......................................................... 16 0 1 2.00 2 0 2 0 0 0 0 0 0
...................................................AAAGTCTGTTATGCTAATGT........................................................................................ 20 0 1 2.00 2 2 0 0 0 0 0 0 0
..................................................AAAAGTCTGTTGTGCTAATGTTTG..................................................................................... 24 1 1 2.00 2 0 0 0 0 0 0 2 0
.......................................................................................CTTGGCATAACCGCTTTTTACTT................................................. 23 0 1 2.00 2 2 0 0 0 0 0 0 0
................................................TCAAAAGTCTGTTATGCTAATGTT....................................................................................... 24 1 1 1.00 1 0 1 0 0 0 0 0 0
..................................................AAAAGTCTGTTATGCTAATGA........................................................................................ 21 1 1 1.00 1 0 0 0 0 0 1 0 0
..................................................AAAAGTCTGTTATGCTAATGTTA...................................................................................... 23 1 1 1.00 1 0 0 0 1 0 0 0 0
......................................................................................ACTTGGCATAACCGCTTTTTAAA.................................................. 23 2 1 1.00 1 0 0 0 0 0 0 1 0
.................................................AAAAAGTCTGTTATGCT............................................................................................. 17 0 1 1.00 1 0 1 0 0 0 0 0 0
......................................................................................ACTTGGCATAACCGC.......................................................... 15 0 1 1.00 1 0 1 0 0 0 0 0 0
................................................TAAAAAGTCTGTTATGCTAATGTT....................................................................................... 24 0 1 1.00 1 0 0 0 0 0 1 0 0
.................................................AAAAAGTCTGTTATGCTAATGTTTAAA................................................................................... 27 3 1 1.00 1 0 0 0 1 0 0 0 0
....................................................................................AAACTTGGCATAACCGCTT........................................................ 19 0 1 1.00 1 1 0 0 0 0 0 0 0
.....................................................................................AACTTGGCATAACCGCTTTTTA.................................................... 22 0 1 1.00 1 1 0 0 0 0 0 0 0
.................................................AAAAAGTCTGTTATGCTAATGTTTA..................................................................................... 25 1 1 1.00 1 0 0 0 1 0 0 0 0
..................................................AAAAGTCTGTTATGCT............................................................................................. 16 0 2 1.00 2 0 2 0 0 0 0 0 0
....................................................................................AAACTTGGCATAACCGCTTTTT..................................................... 22 0 1 1.00 1 1 0 0 0 0 0 0 0
...............................CCATAATCTCAAAATAGT.............................................................................................................. 18 0 2 1.00 2 2 0 0 0 0 0 0 0
..................................................AGAAGTCTGTTATGCTAATGT........................................................................................ 21 1 1 1.00 1 0 1 0 0 0 0 0 0
...................................................AAAGTCTGTTATGCTAATGTTTGT.................................................................................... 24 0 1 1.00 1 1 0 0 0 0 0 0 0
..................................................AAAAGTCTGTTATGCTAATGTTTGA.................................................................................... 25 1 1 1.00 1 0 0 0 0 0 1 0 0
...................................................AAAGTCTGTTATGCTAATGTTT...................................................................................... 22 0 1 1.00 1 1 0 0 0 0 0 0 0
..................................................AAAAGTCTGTTATGCTAATGTTTT..................................................................................... 24 1 1 1.00 1 0 0 0 0 0 0 0 1
..................................................AAAAGTCTGTTATGCTAATGTTAA..................................................................................... 24 2 1 1.00 1 0 0 0 1 0 0 0 0
..................................................AAAAGTCTGTTGTGCTAATGTAA...................................................................................... 23 3 1 1.00 1 0 0 0 0 0 0 1 0
.......................................................................................CTTGGCATAACCGCTTTTTACA.................................................. 22 1 1 1.00 1 0 0 0 1 0 0 0 0
................................................TAAAAAGTCTGTTGTGCTAATGTTA...................................................................................... 25 2 1 1.00 1 0 0 0 0 0 0 0 0
..................................................AAAAGTCTGTTGTGCTAATGTT....................................................................................... 22 1 1 1.00 1 0 0 0 0 0 0 0 0
....................................................AAGTCTGTTATGCTAATGTTTGT.................................................................................... 23 0 1 1.00 1 1 0 0 0 0 0 0 0
..................................................AAAAGTCTGTTATGCTA............................................................................................ 17 0 1 1.00 1 0 1 0 0 0 0 0 0
.................................................AAAAAGTCTGTTGTGCTAATGTTTAA.................................................................................... 26 3 1 1.00 1 0 0 0 1 0 0 0 0
.................................................AAAAAGTCTGTTATGC.............................................................................................. 16 0 2 0.50 1 0 1 0 0 0 0 0 0
..................................................AAAAGTCTGTTCTGTTAATG......................................................................................... 20 2 3 0.33 1 0 0 0 0 0 0 0 1
..................................................AAAAGTCTGTTGTGCT............................................................................................. 16 1 3 0.33 1 0 0 0 1 0 0 0 0
........................................................................................................................................CACGGATTGAAAAAAAA...... 17 1 6 0.17 1 0 0 0 0 0 0 1 0
......................................................................................................................................TGGACAGATTGAGAAAAA....... 18 2 19 0.05 1 0 0 0 0 0 1 0 0

Anti-sense strand reads

CTTGTGTTTTTTTTCAATCTGTGCACTTTGTGGTTAGTTTTCTGCAAATAAGTAAAAAGCGGTTATGCCAAGTTTGTTAAACACACAAACATTAGCATAACAGACTTTTTACTATTTTGAGATTATGGCAATTTGAAGTTCACATTTTTGTCTTTTTTA
************************************....(((.((((..((((((((((.((((((((((((((((.......)))))).))))))))))..))))))))))..)))))))..*********************************** 		Read size # Mismatch Hit Count Total Norm Total
..............................TCTGTAGTTTTCTGCAAATAAG........................................................................................................... 22 3 6 0.17 1
..............................................................................................................ACTATTTGCAGATTAGGGCA............................. 20 3 7 0.14 1
..........................................................GCGGTTATGCCCAGT...................................................................................... 15 1 11 0.09 1
.......................................................................................................ACTATTTACTGTTTTGA....................................... 17 2 20 0.05 1

Re-alignment of all predicted orthologs

Species Coordinate ID Alignment
droSim3 2R:15133465-15133623 - dsi_97 TAAAAAAGACAAAAATGTGAAC-----TTCAAA-TTGCCATAATCT---CAAAATAGTAAAAAGTCTGTTATGCTAATGTTTGTGTGTTTAACAAACTTGGCATAACCGCTTTTTACTTATTTGCAGAAAACTAACCACAAAG-TGCACAGATTGAAAA--AAAAC-ACAAG
droSec2 scaffold_1:14026387-14026546 - dse_115 TAAAAAAGACAAAAATGTGAAC-----TTCAAA-TTGCCATAATCT---CAAACTAGTAAAAAGTCTGTTATGCTAAT-GTTGTGTGTTTAACAAACTTGGCACAACCGCTTTTTATTTATTTGCAGAAAACTAACCACGAAG-TGCACAGATTGAAAAAAAAAAC-ACAAG
dm3 chr2R:16472189-16472340 - dme-mir-2498 T---------------GTGATATTTTTTTCACATTTGCCATAATCTCCTTAAAATAGTA--AAGGCTGTTATGCTTATTTTTGTGTTTTTTACAAAACTGGCATTCCCGTCT--TACTTATTTTCAGAAAACTTACACCAAAG-TGCACAGATTGAAAAAAAAAAC-ACAAA
droEre2 scaffold_4845:10617428-10617560 - der_82 CA-----------------------------AC-TTATAGTATTCC---AAAAATATTT--AAGTCTGTTATGCATACTTTTGTGAGTTTGACAAAATGTGCACAACCGGCTT-ACTGTATTTTTAGAAAACAAACACCTTAGTTGTAAAGAATAAACT--AATTG-TAAAA
droYak3 2R:12723916-12724023 + dya_121 -------------------------------------------------------------AAGTCTGTTCTGCTTAGATTTGTGAATTTGACAAAATCTGCACAGCCGGCTT-AATGTATTTTTAGAAACTTAGCACCTTTTGTGTAAAGAAAAAACT--AAATGTTAAAA

Re-alignment of all predicted orthologs with sRNA reads

Species Read alignment
droSim3
TAAAAAAGACAAAAATGTGAAC-----TTCAAA-TTGCCATAATCT---CAAAATAGTAAAAAGTCTGTTATGCTAATGTTTGTGTGTTTAACAAACTTGGCATAACCGCTTTTTACTTATTTGCAGAAAACTAACCACAAAG-TGCACAGATTGAAAA--AAAAC-ACAAGSizeHit CountTotal NormTotalV044
Embryo		M023
Head		M024
Male-body		M025
Embryo		O001
Testis		O002
Head		M053
Female-body		SRR553485
Ovary		SRR553486
Ovary		SRR553487
Ovary		SRR553488
Ovary		SRR618934
Ovary
...............................................................................................ACTTGGCATAACCGCTTTTTAC.......................................................221776.00776000076510000001
...............................................................................................ACTTGGCATAACCGCTTT...........................................................181131.0013100001292000000
...........................................................AAAAGTCTGTTATGCTAATGTT...........................................................................................221107.0010710008000012419
...........................................................AAAAGTCTGTTATGCTAATGTTT..........................................................................................23188.008800006220021516
................................................................................................CTTGGCATAACCGCTTTTTAC.......................................................21173.00730010711000000
...........................................................AAAAGTCTGTTATGCTAATG.............................................................................................20168.00680050601000002
...........................................................AAAAGTCTGTTATGCTAATGT............................................................................................21163.00630000551000016
...............................................................................................ACTTGGCATAACCGCTTTTTA........................................................21161.00610000610000000
...........................................................AAAAGTCTGTTATGCTAA...............................................................................................18157.00570000570000000
...............................................................................................ACTTGGCATAACCGCTTTT..........................................................19149.00490000462000001
...............................................................................................ACTTGGCATAACCGCTTTTT.........................................................20143.00430000400000003
...........................................................AAAAGTCTGTTATGCTAAT..............................................................................................19134.00340000331000000
...........................................................AAAAGTCTGTTATGCTAATGTTTG.........................................................................................24124.00240000151000008
..........................................................AAAAAGTCTGTTATGCTAATGTTT..........................................................................................24115.0015001040001027
................................................................................................CTTGGCATAACCGCTTTT..........................................................18113.00130000121000000
..........................................................AAAAAGTCTGTTATGCTAATG.............................................................................................21113.0013001091000002
..............................................................................................AACTTGGCATAACCGCTTTTTAC.......................................................23112.00120000120000000
..........................................................AAAAAGTCTGTTATGCTAATGTT...........................................................................................23111.0011000030000008
..........................................................AAAAAGTCTGTTATGCTAATGT............................................................................................2219.009000030000006
................................................................................................CTTGGCATAACCGCTTTTTA........................................................2018.008000080000000
................................................................................................CTTGGCATAACCGCTTTTTACT......................................................2217.007000070000000
..........................................................AAAAAGTCTGTTATGCTA................................................................................................1815.005000050000000
..............................................................................................AACTTGGCATAACCGCTT............................................................1814.004000040000000
..............................................................................................AACTTGGCATAACCGCTTTT..........................................................2014.004000040000000
...............................................................................................ACTTGGCATAACCGCTTTTTACT......................................................2314.004000040000000
..............................................................................................AACTTGGCATAACCGCTTTTT.........................................................2114.004000030000001
...........................................................AAAAGTCTGTTATGCTAATGTTTGT........................................................................................2514.004000030001000
................................................................................................CTTGGCATAACCGCTTTTT.........................................................1914.004000040000000
............................................................AAAGTCTGTTATGCTAATGTTTG.........................................................................................2313.003000030000000
............................................................AAAGTCTGTTATGCTAATGTT...........................................................................................2112.002000020000000
...............................................................................................ACTTGGCATAACCGCTT............................................................1712.002000000000002
..............................................................................................AACTTGGCATAACCGCTTT...........................................................1912.002000020000000
..........................................................AAAAAGTCTGTTATGCTAAT..............................................................................................2012.002000020000000
............................................................AAAGTCTGTTATGCTAATG.............................................................................................1912.002000020000000
...............................................................................................ACTTGGCATAACCGCT.............................................................1612.002000000000002
............................................................AAAGTCTGTTATGCTAATGT............................................................................................2012.002000020000000
................................................................................................CTTGGCATAACCGCTTTTTACTT.....................................................2312.002000020000000
..........................................................AAAAAGTCTGTTATGCT.................................................................................................1711.001000000000001
...............................................................................................ACTTGGCATAACCGC..............................................................1511.001000000000001
.........................................................TAAAAAGTCTGTTATGCTAATGTT...........................................................................................2411.001000000001000
.............................................................................................AAACTTGGCATAACCGCTT............................................................1911.001000010000000
..............................................................................................AACTTGGCATAACCGCTTTTTA........................................................2211.001000010000000
...........................................................AAAAGTCTGTTATGCT.................................................................................................1621.002000000000002
.............................................................................................AAACTTGGCATAACCGCTTTTT.........................................................2211.001000010000000
.....................................CCATAATCT---CAAAATAGT..................................................................................................................1821.002000020000000
............................................................AAAGTCTGTTATGCTAATGTTTGT........................................................................................2411.001000010000000
............................................................AAAGTCTGTTATGCTAATGTTT..........................................................................................2211.001000010000000
.............................................................AAGTCTGTTATGCTAATGTTTGT........................................................................................2311.001000010000000
...........................................................AAAAGTCTGTTATGCTA................................................................................................1711.001000000000001
..........................................................AAAAAGTCTGTTATGC..................................................................................................1620.501000000000001
droSec2
TAAAAAAGACAAAAATGTGAAC-----TTCAAA-TTGCCATAATCT---CAAACTAGTAAAAAGTCTGTTATGCTAAT-GTTGTGTGTTTAACAAACTTGGCACAACCGCTTTTTATTTATTTGCAGAAAACTAACCACGAAG-TGCACAGATTGAAAAAAAAAAC-ACAAGSizeHit CountTotal NormTotalV113
Male-body		V114
Embryo		V115
Head		M054
Female-body
..........................................................AAAAAGTCTGTTATGCTAAT-GTT..........................................................................................23161.0061302731
..........................................................AAAAAGTCTGTTATGCTAAT-GT...........................................................................................22114.001410400
..........................................................AAAAAGTCTGTTATGCTAAT-G............................................................................................2117.0077000
................................................................................................CTTGGCACAACCGCTTTTTAT.......................................................2114.0043100
................................................................................................CTTGGCACAACCGCTTTTTA........................................................2013.0032100
...........................................................AAAAGTCTGTTATGCTAAT-GTT..........................................................................................2212.0020101
...............................................................................................ACTTGGCACAACCGCTTTTTAT.......................................................2212.0022000
............................................................AAAGTCTGTTATGCTAAT-G............................................................................................1911.0011000
...........................................................AAAAGTCTGTTATGCTAAT-GT...........................................................................................2111.0011000
droEre2
CA-----------------------------AC-TTATAGTATTCC---AAAAATATTT--AAGTCTGTTATGCATACTTTTGTGAGTTTGACAAAATGTGCACAACCGGCTT-ACTGTATTTTTAGAAAACAAACACCTTAGTTGTAAAGAATAAACT--AATTG-TAAAASizeHit CountTotal NormTotalV040
Embryo		V060
Head		V107
Male-body		V108
Head		M055
Female-body		M057
Embryo
...............................................................................................AATGTGCACAACCGGCTT-ACT.......................................................21136.003640106151
...............................................................................................AATGTGCACAACCGGCTT-ACTG......................................................22126.00260034190
...............................................................................................AATGTGCACAACCGGCTT-ACTGT.....................................................23124.0024106863
.........................................................TT--AAGTCTGTTATGCATACT.............................................................................................20110.0010003322
.........................................................TT--AAGTCTGTTATGCATACTT............................................................................................2117.007000070
................................................................................................ATGTGCACAACCGGCTT-ACTG......................................................2114.004000040
.........................................................TT--AAGTCTGTTATGCATACTTTT..........................................................................................2312.002000020
................................................................................................ATGTGCACAACCGGCTT-ACT.......................................................2012.002000020
..............................................................................................AAATGTGCACAACCGGCTT-A.........................................................2012.002002000
.........................................................TT--AAGTCTGTTATGCATACTTT...........................................................................................2212.002001100
.........................................................TT--AAGTCTGTTATGCATAC..............................................................................................1911.001001000
...............................................................................................AATGTGCACAACCGGCTT-AC........................................................2011.001001000
.........................................................TT--AAGTCTGTTATGCATACTTTTG.........................................................................................2411.001000010
...............................................................................................AATGTGCACAACCGGCTT-ACTGTA....................................................2411.001000010
...............................................................................................AATGTGCACAACCGGCTT-A.........................................................1911.001000100
..............................................................................................AAATGTGCACAACCGGCTT-ACTGT.....................................................2411.001000001
..........................................................T--AAGTCTGTTATGCATACTTTTG.........................................................................................2311.001000010
.............................................................AAGTCTGTTATGCATACTTTT..........................................................................................2111.001000010
.........................................................TT--AAGTCTGTTATGCATA...............................................................................................1820.501000100
droYak3
-------------------------------------------------------------AAGTCTGTTCTGCTTAGATTTGTGAATTTGACAAAATCTGCACAGCCGGCTT-AATGTATTTTTAGAAACTTAGCACCTTTTGTGTAAAGAAAAAACT--AAATGTTAAAASizeHit CountTotal NormTotalV046
Embryo		V052
Head		V120
Male-body		V058
Head		M026
Head		M043
Female-body		M056
Embryo
...............................................................................................AATCTGCACAGCCGGCTT-AATG......................................................22129.002900806213
...............................................................................................AATCTGCACAGCCGGCTT-AAT.......................................................21125.002510161043
...............................................................................................AATCTGCACAGCCGGCTT-AATGT.....................................................23119.00191071019
................................................................................................ATCTGCACAGCCGGCTT-AATGT.....................................................22111.00110090002
................................................................................................ATCTGCACAGCCGGCTT-AATG......................................................2116.0061010220
................................................................................................ATCTGCACAGCCGGCTT-AAT.......................................................2013.0030010110
...............................................................................................AATCTGCACAGCCGGCTT-A.........................................................1912.0020010010
.................................................................................................TCTGCACAGCCGGCTT-AATG......................................................2011.0010000100

Generated: 12/06/2013 at 02:30 PM