dsi_68

GAACATCAAATGTGTGAATGTAAACTCAATTGTCACTGGTCGTGTCCTCCAAGCGAGCAAAGAAGTGGTATTTGCGTTTCCAAGAACAAATATAACTTCTTTGAAAGCTAGGTGGATTTGCCCAAGTCACCTTGGTATTTATTTTTCAAGTGTGTTCGATTCG *********************************.(((.((.((((.((.(((...(((((((((.(((((((............))))))).)))))))))...))).)).)))).)).)))...**********************************	Read size	# Mismatch	Hit Count	Total Norm	Total	O001 Testis	M024 male body	SRR618934 Ovary	O002 Head	SRR553486 Ovary	SRR553488 Ovary	SRR553485 Ovary	SRR553487 Ovary	M023 head	M053 Female-body
..................................................AAGCGAGCAAAGAAGTGGTATT...........................................................................................	22	0	1	552.00	552	481	30	23	10	4	1	2	1	0	0
..........................................................................................TATAACTTCTTTGAAAGCTAGG...................................................	22	0	1	407.00	407	355	11	14	8	4	6	4	3	0	2
..................................................AAGCGAGCAAAGAAGTGGTA.............................................................................................	20	0	1	230.00	230	225	0	2	3	0	0	0	0	0	0
..................................................AAGCGAGCAAAGAAGTGGTAT............................................................................................	21	0	1	186.00	186	179	2	2	3	0	0	0	0	0	0
..........................................................................................TATAACTTCTTTGAAAGC.......................................................	18	0	1	136.00	136	133	1	0	2	0	0	0	0	0	0
..................................................AAGCGAGCAAAGAAGTGG...............................................................................................	18	0	1	118.00	118	116	0	1	1	0	0	0	0	0	0
..........................................................................................TATAACTTCTTTGAAAGCTA.....................................................	20	0	1	89.00	89	88	0	0	1	0	0	0	0	0	0
..........................................................................................TATAACTTCTTTGAAAGCTAG....................................................	21	0	1	83.00	83	82	0	0	1	0	0	0	0	0	0
..........................................................................................TATAACTTCTTTGAAAGCT......................................................	19	0	1	63.00	63	58	3	1	1	0	0	0	0	0	0
..................................................AAGCGAGCAAAGAAGTGGTATTT..........................................................................................	23	0	1	57.00	57	52	2	2	1	0	0	0	0	0	0
..........................................................................................TATAACTTCTTTGAAAGCTAGGT..................................................	23	0	1	47.00	47	47	0	0	0	0	0	0	0	0	0
..................................................AAGCGAGCAAAGAAGTGGT..............................................................................................	19	0	1	38.00	38	38	0	0	0	0	0	0	0	0	0
.........................................................................................ATATAACTTCTTTGAAAGCTAG....................................................	22	0	1	24.00	24	23	0	0	1	0	0	0	0	0	0
...................................................AGCGAGCAAAGAAGTGGTATTT..........................................................................................	22	0	1	16.00	16	13	3	0	0	0	0	0	0	0	0
.........................................................................................ATATAACTTCTTTGAAAGCTAGG...................................................	23	0	1	10.00	10	9	0	0	0	0	1	0	0	0	0
...................................................AGCGAGCAAAGAAGTGGTATT...........................................................................................	21	0	1	9.00	9	8	1	0	0	0	0	0	0	0	0
...........................................................................................ATAACTTCTTTGAAAGCTAGG...................................................	21	0	1	7.00	7	7	0	0	0	0	0	0	0	0	0
...................................................AGCGAGCAAAGAAGTGGTA.............................................................................................	19	0	1	6.00	6	6	0	0	0	0	0	0	0	0	0
.........................................................................................ATATAACTTCTTTGAAAG........................................................	18	0	1	6.00	6	6	0	0	0	0	0	0	0	0	0
................................TCACTGGTCGTGTCCTCC.................................................................................................................	18	0	1	6.00	6	2	3	0	0	0	0	0	0	1	0
.........................................................................................ATATAACTTCTTTGAAAGCTA.....................................................	21	0	1	6.00	6	5	1	0	0	0	0	0	0	0	0
..............................................................................................ACTTCTTTGAAAGCTAGG...................................................	18	0	1	4.00	4	0	0	4	0	0	0	0	0	0	0
.........................................................................................ATATAACTTCTTTGAAAGCT......................................................	20	0	1	4.00	4	1	2	0	0	0	0	0	0	1	0
.........................................................................................ATATAACTTCTTTGAAAGC.......................................................	19	0	1	3.00	3	3	0	0	0	0	0	0	0	0	0
...................................................AGCGAGCAAAGAAGTGGTAT............................................................................................	20	0	1	3.00	3	3	0	0	0	0	0	0	0	0	0
...............................GTCACTGGTCGTGTCCTCC.................................................................................................................	19	0	1	2.00	2	1	1	0	0	0	0	0	0	0	0
...........................................................................................ATAACTTCTTTGAAAGCTAG....................................................	20	0	1	2.00	2	2	0	0	0	0	0	0	0	0	0
..................................................AAGCGGGCAAAGAAGTGGTATT...........................................................................................	22	1	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.....................................................CGAGCAAAGAAGTGGTAT............................................................................................	18	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0
..................................................AAGCGAGCAAAGAAGT.................................................................................................	16	0	1	1.00	1	0	0	1	0	0	0	0	0	0	0
................................TCACTGGTCGTGTCCTCCA................................................................................................................	19	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0
..............................................................................................ACTTCTTTGAAAGCTAGGT..................................................	19	0	1	1.00	1	0	0	1	0	0	0	0	0	0	0
.....................................................CGAGCAAAGAAGTGGTATTTGCG.......................................................................................	23	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0
..................................................AAGCGAGCAAAGAAGTGGTACT...........................................................................................	22	1	1	1.00	1	0	1	0	0	0	0	0	0	0	0
......................................................................................CAAATATAACTTCTTTGAAAGC.......................................................	22	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0
...........................................................................................ATAACTTCTTTGAAAGCTAGGT..................................................	22	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0
........................................................GCAAAGAAGTGGTATT...........................................................................................	16	0	1	1.00	1	0	0	1	0	0	0	0	0	0	0
..........................................................................................TATAACTTCTTTGAAAGCTAGGA..................................................	23	1	1	1.00	1	0	0	1	0	0	0	0	0	0	0
..................................................AATCGAGCAAAGAAGTGGTATT...........................................................................................	22	1	1	1.00	1	0	0	0	0	1	0	0	0	0	0
.....................................................CGAGCAAAGAAGTGGTATTTGCGT......................................................................................	24	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0
..................................................AAGCGAGCAAAGAAGTGGTATTTGC........................................................................................	25	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0
..................................................AAGCGAGCAAAGAAGTGGTATTTGCG.......................................................................................	26	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0
...........................................................................................ATAACTTCTTTGAAAGCTA.....................................................	19	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0
......................................................GAGCAAAGAAGTGGT..............................................................................................	15	0	1	1.00	1	0	0	1	0	0	0	0	0	0	0
..................................................AAGCGAGCAAAGAAG..................................................................................................	15	0	2	0.50	1	0	0	1	0	0	0	0	0	0	0
..................................................AAGCGAGCAAAGAAGTGC...............................................................................................	18	1	2	0.50	1	0	0	1	0	0	0	0	0	0	0
................................TCACTGGTCGAGTCGTCC.................................................................................................................	18	2	3	0.33	1	0	0	0	0	0	1	0	0	0	0
..........................................................................................TATAACTTCTTGTAAAGCT......................................................	19	2	5	0.20	1	0	0	1	0	0	0	0	0	0	0
................................TCACTGGTCGAGTCGTC..................................................................................................................	17	2	13	0.15	2	0	0	0	0	0	1	0	1	0	0
...........................................................................................ATAGCTTTTTTGAAAGCT......................................................	18	2	15	0.07	1	0	0	1	0	0	0	0	0	0	0
....................................TGGTCGTGGCCTCCA................................................................................................................	15	1	16	0.06	1	0	0	0	0	0	1	0	0	0	0
.....ACAGATGTGTGAATGTA.............................................................................................................................................	17	2	20	0.05	1	0	0	1	0	0	0	0	0	0	0
..................................ACTGGTCGAGTCTTCC.................................................................................................................	16	2	20	0.05	1	0	0	0	0	0	1	0	0	0	0
................................TCACAGGTCGAGTCCT...................................................................................................................	16	2	20	0.05	1	0	0	0	0	0	1	0	0	0	0

CGAATCGAACACACTTGAAAAATAAATACCAAGGTGACTTGGGCAAATCCACCTAGCTTTCAAAGAAGTTATATTTGTTCTTGGAAACGCAAATACCACTTCTTTGCTCGCTTGGAGGACACGACCAGTGACAATTGAGTTTACATTCACACATTTGATGTTC **********************************...(((.((.((((.((.(((...(((((((((.(((((((............))))))).)))))))))...))).)).)))).)).))).*********************************	Read size	# Mismatch	Hit Count	Total Norm	Total	SRR553487 Ovary	O001 Testis	SRR553485 Ovary	SRR553488 Ovary
......................................TTGGGCAAATCCACCTAGCTTTCAAAGA.................................................................................................	28	0	1	1.00	1	0	0	1	0
.......................................................................................................TTGCTCGCTTGGAGGACACGACCAG...................................	25	0	1	1.00	1	0	0	0	1
................................................................................................................TGGAGGACACGACCAGTGACAATTGA.........................	26	0	1	1.00	1	1	0	0	0
..............................................................................................ACCACTTCTTTGCTCGCT...................................................	18	0	1	1.00	1	0	1	0	0
......................................................................................................TTTGCTCGCTTGGAGGACACGACCAG...................................	26	0	1	1.00	1	1	0	0	0
............................................................................................ATACCACTTCTTCGCTCGT....................................................	19	2	4	0.25	1	1	0	0	0
............................................................................................................CGCTTGGAGGACAAGATCC....................................	19	3	20	0.05	1	0	0	0	0

Species	Coordinate	ID	Alignment
droSim3	X:15011405-15011567 +	dsi_68	GAACATCAAATGTGTGAA-----T---------------------------------------G--------------------TAAACT--C-AATTGTCACTGGTCGTGTCCTCCAAGCGAGCAAAGAAGTGGTATTTGCGTT-TC---CAA--GAACAAATATAACTTCTTTGAAAGCTAGGTGGATTTGCCC-AAGT-CACCTTGGTATTTATT--------TTTC-AAGTGTGTTCGAT-----------TCG
droSec2	scaffold_8:1728083-1728248 +	dse_40	GAGCATCAAATGAGTGAA-----TCA-------------------------------------------GG-------------TAAACT--C-GATTGTCACTGGTCGTGTCCTCCAAGCGAGCAAAGAAGTAGTATTTGCGTT-TC---CAA--GAACAAATATAACTTCTTTGAAAGCTAGGTGGATTTGCCC-AAGT-CTCCTTGGTATTTATT--------TTTC-AAGTGTGTTCGAT-----------TGG
dm3	chrX:19445674-19445855 +	dme-mir-974	GAACATCAAATGCGTGAA-----TC---------------------------------------C----CAGGTCCAGG-----TAAGCT--C-AATTGTCACCGGTCATGTCCTCCAAGCGAGCAAAGAAGTAGTATTTGTGTT-TC---CAA--GAGCAAATATAACTTCATTGGAAGCTAAGTGGATTTGCCC-AAAT-CAGCTTGGTATTTATTTTTTATTTTTTC-GAGTGTGTTCGAT-----------TGG
droEre2	scaffold_4690:9682415-9682583 +	der_30	AAACATGAATCGAGTGAA-----TCA-------------------------------------------GGAAAACAGAA-----CCACT--A-ACTCAGCACTGGGCATGTCCTCCAAGCGAGCAAAGAAGTAGTATTTGTGTT-CC---AAA--AGTCAAATATAACTTTTTTGGAAGCTACGGGGATTTGCCC-AAGC-CAACTTGCTGC-------------TTTT-AAGTGTGTTCGAT-----------TGG
droEug1	scf7180000409109:6679-6863 -		AAACATAAATAAGGTGA------TA--------------------GTGCTTAAAA------------CTAAA--ACATAATAATTAAGAT--T-AAGAATAATTGGATATGTCCTCTAAGCGAGCAAAGAAGTAGTATTTGTTTA-TT-----A--AGTCGAATATAACTTTTTTGGAAGCTAAGCGGATGTATCT-AAACCCAGCTTGATGTTT-TT--------TTTT-AATTATGTGTGAA-----------ATA
droBia1	scf7180000302069:1157324-1157471 -		ATAAA-AAACAGAGTGA--TGAGACAAGGCAA-----------------------------TAC---------------------AAATTTAGAATTTAGAAAAGGTCATCTCCTCCAAGCGAGCAAAGAAGTAGTATTTGTTTG-TT---AAA--AGTCAAATATAACTTTTTTGGAAGCTAAGAGGATTTATCC-TAAC-CAGCTTG-------------------------------------------------
droTak1	scf7180000415169:850560-850732 -		GCATACAAATTGAGTGA--------------------------------------------TAAT----A-----------------ATTTGGAACTTCGAATAGGTCATCTCCTCTAAGCGAGCAAAGAAGTAGTATTTGTTTG-TT--GAAG--AGTCAAATATAACTTTTTTGGAAGCTAAGAGGATTTGTCC-TAAG-CAGCTTGGTTA-------------TTTCGAATTATTCGTTAATTAAGGCACGCTCA
droEle1	scf7180000490751:1106970-1107109 +		ACACTCAAATGGAGCAA------A--------------------------------------AAA----A-----AATAC-----GGGCT--G-ACTCGGAAAAGGTCATTTCCTCCAAGCGAGCAAAGAAGTAGTATTTGTTTG-TTCTGAAA--AGTCAAATATAACTTTTTTGAAAGCTACGAGGATTTGTCCCAAAT-CAC-----------------------------------------------------
droRho1	scf7180000761121:198590-198725 -		ATACTCAA-CTGA----A----GAGA-----------------------------------AAAC----AA-------------CGAGCT--T-TCTCGAAATAGGTCATCTCCTCTAAGCGAGCAAAGAAGTAGTATTTGTTTG-TTCTGAAA--AGTCAAATATAACTTTTTTGAAAGCTACGAGGATTTGTTC-CATT-AAC-----------------------------------------------------
droFic1	scf7180000454073:1724048-1724174 +		TAAAACAAAGTG----C--TAAGG-------A-----------------------------TACG----C-----------------AATTAAAAACAAAAAAGGAGCATTTCCCCTAAGCGAGCAAAGAAGTAGTATTTGTTCG-TGCTGAAA--ACTCAAATATAACTTTTTTGAAAGCTAAAAGGATTTG-----------------------------------------------------------------
droKik1	scf7180000302517:1176315-1176463 +		GAACCCCAAGGA-----------TC--------------------CTGCCTAGAACGGTGAGTATAGCCGGG--ACAT----------CT----CCGCCAAGCTGGTCTTTTCCTCTAAGCGAGCAAAGAAGTGGTATTTGTTGC-ACTTGAAATTATTCAAATATAACTTTTTTGGAAGCTAGAAGGAGTTGCCC-A------------------------------------------------------------
droAna3	scaffold_13417:2478487-2478643 -	dan_39	AAACATGAACCGCGG--CTCAAATCAATCCAATCCGAATCCAATCGCGG------------TTGC----A------------AT---CCT--G-GCTTGGGGTAGGTCATCTCCTCTAAGCGAGCAAAGAAGTAGTATTTGTTTG-CTTTTAAT--GGTCAAATATAACTTTTTTGGAAGCTACGAGGAGTTGTCC--------------------------------------------------------------
droWil2	scf2_1100000004909:7946590-7946711 +	dwi_59	GCACACAAGAAAAGCGAA-----TC--------------------------------------------------------------ATT--CATTTTTCCAATGGCCATATCCTCTAAGCGAGCAAGAAAGTGGTATTTGTCTT-T---GGAA--ATTCAAATATAACTTTTTTGGAAGCTACAAGGCAATGTCC-A------------------------------------------------------------
droVir3	scaffold_13042:917580-917659 -	dvi_139	CAAT--------------------------------------------------------------------------------------------TATTAACTGGGCATTCCCTCTGAGCGAGCAGAGAAGTATTATTTGCGTGAATC--TGA--TATCAAATAAGACTTTTTTG----------------------------------------------------------------------------------

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droSim3

GAACATCAAATGTGTGAA-----T---------------------------------------G--------------------TAAACT--C-AATTGTCACTGGTCGTGTCCTCCAAGCGAGCAAAGAAGTGGTATTTGCGTT-TC---CAA--GAACAAATATAACTTCTTTGAAAGCTAGGTGGATTTGCCC-AAGT-CACCTTGGTATTTATT--------TTTC-AAGTGTGTTCGAT-----------TCG	Size	Hit Count	Total Norm	Total	V044 Embryo	M023 Head	M024 Male-body	M025 Embryo	O001 Testis	O002 Head	M053 Female-body	SRR553485 Ovary	SRR553486 Ovary	SRR553487 Ovary	SRR553488 Ovary	SRR618934 Ovary
.....................................................................................................................AAGCGAGCAAAGAAGTGGTATT.......................................................................................................................	22	1	552.00	552	0	0	30	0	481	10	0	2	4	1	1	23
...................................................................................................................................................................TATAACTTCTTTGAAAGCTAGG.........................................................................	22	1	407.00	407	0	0	11	0	355	8	2	4	4	3	6	14
.....................................................................................................................AAGCGAGCAAAGAAGTGGTA.........................................................................................................................	20	1	230.00	230	0	0	0	0	225	3	0	0	0	0	0	2
.....................................................................................................................AAGCGAGCAAAGAAGTGGTAT........................................................................................................................	21	1	186.00	186	0	0	2	0	179	3	0	0	0	0	0	2
...................................................................................................................................................................TATAACTTCTTTGAAAGC.............................................................................	18	1	136.00	136	0	0	1	0	133	2	0	0	0	0	0	0
.....................................................................................................................AAGCGAGCAAAGAAGTGG...........................................................................................................................	18	1	118.00	118	0	0	0	0	116	1	0	0	0	0	0	1
...................................................................................................................................................................TATAACTTCTTTGAAAGCTA...........................................................................	20	1	89.00	89	0	0	0	0	88	1	0	0	0	0	0	0
...................................................................................................................................................................TATAACTTCTTTGAAAGCTAG..........................................................................	21	1	83.00	83	0	0	0	0	82	1	0	0	0	0	0	0
...................................................................................................................................................................TATAACTTCTTTGAAAGCT............................................................................	19	1	63.00	63	0	0	3	0	58	1	0	0	0	0	0	1
.....................................................................................................................AAGCGAGCAAAGAAGTGGTATTT......................................................................................................................	23	1	57.00	57	0	0	2	0	52	1	0	0	0	0	0	2
...................................................................................................................................................................TATAACTTCTTTGAAAGCTAGGT........................................................................	23	1	47.00	47	0	0	0	0	47	0	0	0	0	0	0	0
.....................................................................................................................AAGCGAGCAAAGAAGTGGT..........................................................................................................................	19	1	38.00	38	0	0	0	0	38	0	0	0	0	0	0	0
..................................................................................................................................................................ATATAACTTCTTTGAAAGCTAG..........................................................................	22	1	24.00	24	0	0	0	0	23	1	0	0	0	0	0	0
......................................................................................................................AGCGAGCAAAGAAGTGGTATTT......................................................................................................................	22	1	16.00	16	0	0	3	0	13	0	0	0	0	0	0	0
..................................................................................................................................................................ATATAACTTCTTTGAAAGCTAGG.........................................................................	23	1	10.00	10	0	0	0	0	9	0	0	0	0	0	1	0
......................................................................................................................AGCGAGCAAAGAAGTGGTATT.......................................................................................................................	21	1	9.00	9	0	0	1	0	8	0	0	0	0	0	0	0
....................................................................................................................................................................ATAACTTCTTTGAAAGCTAGG.........................................................................	21	1	7.00	7	0	0	0	0	7	0	0	0	0	0	0	0
......................................................................................................................AGCGAGCAAAGAAGTGGTA.........................................................................................................................	19	1	6.00	6	0	0	0	0	6	0	0	0	0	0	0	0
..................................................................................................................................................................ATATAACTTCTTTGAAAG..............................................................................	18	1	6.00	6	0	0	0	0	6	0	0	0	0	0	0	0
...................................................................................................TCACTGGTCGTGTCCTCC.............................................................................................................................................	18	1	6.00	6	0	1	3	0	2	0	0	0	0	0	0	0
..................................................................................................................................................................ATATAACTTCTTTGAAAGCTA...........................................................................	21	1	6.00	6	0	0	1	0	5	0	0	0	0	0	0	0
.......................................................................................................................................................................ACTTCTTTGAAAGCTAGG.........................................................................	18	1	4.00	4	0	0	0	0	0	0	0	0	0	0	0	4
..................................................................................................................................................................ATATAACTTCTTTGAAAGCT............................................................................	20	1	4.00	4	0	1	2	0	1	0	0	0	0	0	0	0
..................................................................................................................................................................ATATAACTTCTTTGAAAGC.............................................................................	19	1	3.00	3	0	0	0	0	3	0	0	0	0	0	0	0
......................................................................................................................AGCGAGCAAAGAAGTGGTAT........................................................................................................................	20	1	3.00	3	0	0	0	0	3	0	0	0	0	0	0	0
..................................................................................................GTCACTGGTCGTGTCCTCC.............................................................................................................................................	19	1	2.00	2	0	0	1	0	1	0	0	0	0	0	0	0
....................................................................................................................................................................ATAACTTCTTTGAAAGCTAG..........................................................................	20	1	2.00	2	0	0	0	0	2	0	0	0	0	0	0	0
........................................................................................................................CGAGCAAAGAAGTGGTAT........................................................................................................................	18	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
.....................................................................................................................AAGCGAGCAAAGAAGT.............................................................................................................................	16	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	1
...................................................................................................TCACTGGTCGTGTCCTCCA............................................................................................................................................	19	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
.......................................................................................................................................................................ACTTCTTTGAAAGCTAGGT........................................................................	19	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	1
........................................................................................................................CGAGCAAAGAAGTGGTATTTGCG...................................................................................................................	23	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
...............................................................................................................................................................CAAATATAACTTCTTTGAAAGC.............................................................................	22	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
....................................................................................................................................................................ATAACTTCTTTGAAAGCTAGGT........................................................................	22	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
...........................................................................................................................GCAAAGAAGTGGTATT.......................................................................................................................	16	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	1
........................................................................................................................CGAGCAAAGAAGTGGTATTTGCGT..................................................................................................................	24	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
.....................................................................................................................AAGCGAGCAAAGAAGTGGTATTTGC....................................................................................................................	25	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
.....................................................................................................................AAGCGAGCAAAGAAGTGGTATTTGCG...................................................................................................................	26	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
....................................................................................................................................................................ATAACTTCTTTGAAAGCTA...........................................................................	19	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
.........................................................................................................................GAGCAAAGAAGTGGT..........................................................................................................................	15	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	1
.....................................................................................................................AAGCGAGCAAAGAAG..............................................................................................................................	15	2	0.50	1	0	0	0	0	0	0	0	0	0	0	0	1

droSec2

GAGCATCAAATGAGTGAA-----TCA-------------------------------------------GG-------------TAAACT--C-GATTGTCACTGGTCGTGTCCTCCAAGCGAGCAAAGAAGTAGTATTTGCGTT-TC---CAA--GAACAAATATAACTTCTTTGAAAGCTAGGTGGATTTGCCC-AAGT-CTCCTTGGTATTTATT--------TTTC-AAGTGTGTTCGAT-----------TGG	Size	Hit Count	Total Norm	Total	V113 Male-body	V114 Embryo	V115 Head	M054 Female-body
.....................................................................................................................AAGCGAGCAAAGAAGTAGTATT.......................................................................................................................	22	1	1247.00	1247	1190	11	44	2
.....................................................................................................................AAGCGAGCAAAGAAGTAGTATTT......................................................................................................................	23	1	186.00	186	181	1	3	1
...................................................................................................................................................................TATAACTTCTTTGAAAGCTAGG.........................................................................	22	1	105.00	105	86	10	5	4
.....................................................................................................................AAGCGAGCAAAGAAGTAGTAT........................................................................................................................	21	1	35.00	35	33	0	1	1
......................................................................................................................AGCGAGCAAAGAAGTAGTATTT......................................................................................................................	22	1	18.00	18	17	1	0	0
...................................................................................................................................................................TATAACTTCTTTGAAAGCTAG..........................................................................	21	1	6.00	6	6	0	0	0
.....................................................................................................................AAGCGAGCAAAGAAGTAGTA.........................................................................................................................	20	1	5.00	5	5	0	0	0
......................................................................................................................AGCGAGCAAAGAAGTAGTATT.......................................................................................................................	21	1	3.00	3	1	0	1	1
...................................................................................................TCACTGGTCGTGTCCTCC.............................................................................................................................................	18	1	2.00	2	0	1	1	0
.....................................................................................................................AAGCGAGCAAAGAAGTAGT..........................................................................................................................	19	1	2.00	2	2	0	0	0
...................................................................................................................................................................TATAACTTCTTTGAAAGCTAGGT........................................................................	23	1	2.00	2	1	1	0	0
..................................................................................................................................................................ATATAACTTCTTTGAAAGCTAGG.........................................................................	23	1	1.00	1	1	0	0	0
....................................................................................................................CAAGCGAGCAAAGAAGTAGTAT........................................................................................................................	22	1	1.00	1	1	0	0	0
.......................................................................................................................GCGAGCAAAGAAGTAGTATT.......................................................................................................................	20	1	1.00	1	0	0	0	1
..................................................................................................GTCACTGGTCGTGTCCTCC.............................................................................................................................................	19	1	1.00	1	0	1	0	0
..................................................................................................................................................................ATATAACTTCTTTGAAAGCTA...........................................................................	21	1	1.00	1	0	1	0	0
......................................................................................................................AGCGAGCAAAGAAGTAGTATTTG.....................................................................................................................	23	1	1.00	1	1	0	0	0
.........................................................................................................................GAGCAAAGAAGTAGTATT.......................................................................................................................	18	1	1.00	1	1	0	0	0
....................................................................................................................CAAGCGAGCAAAGAAGTAGTATT.......................................................................................................................	23	1	1.00	1	1	0	0	0

droEre2

AAACATGAATCGAGTGAA-----TCA-------------------------------------------GGAAAACAGAA-----CCACT--A-ACTCAGCACTGGGCATGTCCTCCAAGCGAGCAAAGAAGTAGTATTTGTGTT-CC---AAA--AGTCAAATATAACTTTTTTGGAAGCTACGGGGATTTGCCC-AAGC-CAACTTGCTGC-------------TTTT-AAGTGTGTTCGAT-----------TGG	Size	Hit Count	Total Norm	Total	V040 Embryo	V060 Head	V107 Male-body	V108 Head	M055 Female-body	M057 Embryo
.....................................................................................................................AAGCGAGCAAAGAAGTAGTATT.......................................................................................................................	22	1	3284.00	3284	0	7	2953	319	5	0
.....................................................................................................................AAGCGAGCAAAGAAGTAGTATTT......................................................................................................................	23	1	1614.00	1614	0	11	1458	143	2	0
...................................................................................................................................................................TATAACTTTTTTGGAAGCTACG.........................................................................	22	1	178.00	178	0	5	155	12	6	0
.....................................................................................................................AAGCGAGCAAAGAAGTAGTAT........................................................................................................................	21	1	85.00	85	0	0	84	1	0	0
.....................................................................................................................AAGCGAGCAAAGAAGTAGTA.........................................................................................................................	20	1	10.00	10	0	0	10	0	0	0
..................................................................................................................................................................ATATAACTTTTTTGGAAGCTACG.........................................................................	23	1	6.00	6	0	0	4	2	0	0
......................................................................................................................AGCGAGCAAAGAAGTAGTATT.......................................................................................................................	21	1	5.00	5	0	0	3	0	2	0
.....................................................................................................................AAGCGAGCAAAGAAGTAGT..........................................................................................................................	19	1	4.00	4	0	0	4	0	0	0
.....................................................................................................................AAGCGAGCAAAGAAGTAGTATTTG.....................................................................................................................	24	1	3.00	3	0	0	3	0	0	0
........................................................................................................................CGAGCAAAGAAGTAGTATT.......................................................................................................................	19	1	2.00	2	0	0	1	1	0	0
...................................................................................................................................................................TATAACTTTTTTGGAAGCTA...........................................................................	20	1	2.00	2	0	0	2	0	0	0
......................................................................................................................AGCGAGCAAAGAAGTAGTATTT......................................................................................................................	22	1	2.00	2	0	0	2	0	0	0
.......................................................................................................................GCGAGCAAAGAAGTAGTATTT......................................................................................................................	21	1	1.00	1	0	0	1	0	0	0
....................................................................................................................CAAGCGAGCAAAGAAGTAGTATT.......................................................................................................................	23	1	1.00	1	0	0	1	0	0	0
....................................................................................................................CAAGCGAGCAAAGAAGTAGTATTT......................................................................................................................	24	1	1.00	1	0	0	0	1	0	0
......................................................................................................................AGCGAGCAAAGAAGTAGTAT........................................................................................................................	20	1	1.00	1	0	0	1	0	0	0
.........................................................................................................................GAGCAAAGAAGTAGTATT.......................................................................................................................	18	1	1.00	1	0	0	1	0	0	0
.....................................................................................................................................................................TAACTTTTTTGGAAGCTACG.........................................................................	20	1	1.00	1	0	0	1	0	0	0
.......................................................................................................................GCGAGCAAAGAAGTAGTATT.......................................................................................................................	20	1	1.00	1	0	0	1	0	0	0
....................................................................................................................................................................ATAACTTTTTTGGAAGCTACG.........................................................................	21	1	1.00	1	0	0	1	0	0	0
...................................................................................................................................................................TATAACTTTTTTGGAAGCTAC..........................................................................	21	1	1.00	1	0	0	1	0	0	0
..................................................................................................................................................................ATATAACTTTTTTGGAAGCTAC..........................................................................	22	1	1.00	1	0	0	1	0	0	0

droEug1

AAACATAAATAAGGTGA------TA--------------------GTGCTTAAAA------------CTAAA--ACATAATAATTAAGAT--T-AAGAATAATTGGATATGTCCTCTAAGCGAGCAAAGAAGTAGTATTTGTTTA-TT-----A--AGTCGAATATAACTTTTTTGGAAGCTAAGCGGATGTATCT-AAACCCAGCTTGATGTTT-TT--------TTTT-AATTATGTGTGAA-----------ATA	Size	Hit Count	Total Norm	Total
No Reads

droBia1

ATAAA-AAACAGAGTGA--TGAGACAAGGCAA-----------------------------TAC---------------------AAATTTAGAATTTAGAAAAGGTCATCTCCTCCAAGCGAGCAAAGAAGTAGTATTTGTTTG-TT---AAA--AGTCAAATATAACTTTTTTGGAAGCTAAGAGGATTTATCC-TAAC-CAGCTTG-------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droTak1

GCATACAAATTGAGTGA--------------------------------------------TAAT----A-----------------ATTTGGAACTTCGAATAGGTCATCTCCTCTAAGCGAGCAAAGAAGTAGTATTTGTTTG-TT--GAAG--AGTCAAATATAACTTTTTTGGAAGCTAAGAGGATTTGTCC-TAAG-CAGCTTGGTTA-------------TTTCGAATTATTCGTTAATTAAGGCACGCTCA	Size	Hit Count	Total Norm	Total
No Reads

droEle1

ACACTCAAATGGAGCAA------A--------------------------------------AAA----A-----AATAC-----GGGCT--G-ACTCGGAAAAGGTCATTTCCTCCAAGCGAGCAAAGAAGTAGTATTTGTTTG-TTCTGAAA--AGTCAAATATAACTTTTTTGAAAGCTACGAGGATTTGTCCCAAAT-CAC-----------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droRho1

ATACTCAA-CTGA----A----GAGA-----------------------------------AAAC----AA-------------CGAGCT--T-TCTCGAAATAGGTCATCTCCTCTAAGCGAGCAAAGAAGTAGTATTTGTTTG-TTCTGAAA--AGTCAAATATAACTTTTTTGAAAGCTACGAGGATTTGTTC-CATT-AAC-----------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droFic1

TAAAACAAAGTG----C--TAAGG-------A-----------------------------TACG----C-----------------AATTAAAAACAAAAAAGGAGCATTTCCCCTAAGCGAGCAAAGAAGTAGTATTTGTTCG-TGCTGAAA--ACTCAAATATAACTTTTTTGAAAGCTAAAAGGATTTG-----------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droKik1

GAACCCCAAGGA-----------TC--------------------CTGCCTAGAACGGTGAGTATAGCCGGG--ACAT----------CT----CCGCCAAGCTGGTCTTTTCCTCTAAGCGAGCAAAGAAGTGGTATTTGTTGC-ACTTGAAATTATTCAAATATAACTTTTTTGGAAGCTAGAAGGAGTTGCCC-A------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droAna3

AAACATGAACCGCGG--CTCAAATCAATCCAATCCGAATCCAATCGCGG------------TTGC----A------------AT---CCT--G-GCTTGGGGTAGGTCATCTCCTCTAAGCGAGCAAAGAAGTAGTATTTGTTTG-CTTTTAAT--GGTCAAATATAACTTTTTTGGAAGCTACGAGGAGTTGTCC--------------------------------------------------------------	Size	Hit Count	Total Norm	Total	V105 Male-body	V106 Head	V055 Head	V039 Embryo	M044 Female-body	M058 Embryo
.....................................................................................................................AAGCGAGCAAAGAAGTAGTATT.......................................................................................................................	22	1	355.00	355	294	37	19	0	4	1
.....................................................................................................................AAGCGAGCAAAGAAGTAGTATTT......................................................................................................................	23	1	114.00	114	91	17	5	0	1	0
...................................................................................................................................................................TATAACTTTTTTGGAAGCTACG.........................................................................	22	1	80.00	80	74	0	5	0	1	0
.....................................................................................................................AAGCGAGCAAAGAAGTAGTAT........................................................................................................................	21	1	54.00	54	51	1	2	0	0	0
......................................................................................................................AGCGAGCAAAGAAGTAGTATTT......................................................................................................................	22	1	9.00	9	5	4	0	0	0	0
......................................................................................................................AGCGAGCAAAGAAGTAGTATT.......................................................................................................................	21	1	4.00	4	4	0	0	0	0	0
.....................................................................................................................AAGCGAGCAAAGAAGTAGTA.........................................................................................................................	20	1	3.00	3	2	0	1	0	0	0
...................................................................................................................................................................TATAACTTTTTTGGAAGCTAC..........................................................................	21	1	2.00	2	2	0	0	0	0	0
..................................................................................................................................................................ATATAACTTTTTTGGAAGCTAC..........................................................................	22	1	2.00	2	2	0	0	0	0	0
...................................................................................................................................................................TATAACTTTTTTGGAAGCTA...........................................................................	20	1	1.00	1	1	0	0	0	0	0
...................................................................................................................................................................TATAACTTTTTTGGAAGCTACGA........................................................................	23	1	1.00	1	1	0	0	0	0	0
..................................................................................................................................................................ATATAACTTTTTTGGAAGCTA...........................................................................	21	1	1.00	1	1	0	0	0	0	0

droWil2

GCACACAAGAAAAGCGAA-----TC--------------------------------------------------------------ATT--CATTTTTCCAATGGCCATATCCTCTAAGCGAGCAAGAAAGTGGTATTTGTCTT-T---GGAA--ATTCAAATATAACTTTTTTGGAAGCTACAAGGCAATGTCC-A------------------------------------------------------------	Size	Hit Count	Total Norm	Total	V117 Male-body	V118 Embryo	V119 Head	M020 Head	M045 Female-body
.....................................................................................................................AAGCGAGCAAGAAAGTGGTATT.......................................................................................................................	22	1	662.00	662	649	7	4	1	1
.....................................................................................................................AAGCGAGCAAGAAAGTGGTATTT......................................................................................................................	23	1	150.00	150	144	3	2	0	1
.....................................................................................................................AAGCGAGCAAGAAAGTGGTAT........................................................................................................................	21	1	51.00	51	50	0	1	0	0
.....................................................................................................................AAGCGAGCAAGAAAGTGGTA.........................................................................................................................	20	1	5.00	5	5	0	0	0	0
...................................................................................................................................................................TATAACTTTTTTGGAAGCTACA.........................................................................	22	1	5.00	5	4	1	0	0	0
......................................................................................................................AGCGAGCAAGAAAGTGGTATTT......................................................................................................................	22	1	2.00	2	2	0	0	0	0

droVir3

CAAT--------------------------------------------------------------------------------------------TATTAACTGGGCATTCCCTCTGAGCGAGCAGAGAAGTATTATTTGCGTGAATC--TGA--TATCAAATAAGACTTTTTTG----------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	V116 Male-body	V053 Head	V047 Embryo	M027 Male-body	M028 Head	M047 Female-body	M061 Embryo	SRR060654 Ovary	SRR060655 Testis	SRR060656 Ovary	SRR060657 Testis	SRR060658 Ovary	SRR060659 Testis	SRR060660 Ovary	SRR060661 Embryo	SRR060662 Embryo	SRR060663 Embryo	SRR060664 Male-body	SRR060665 Female-body	SRR060666 Male-body	SRR060667 Female-body	SRR060668 Male-body	SRR060669 Female-body	SRR060670 Testis	SRR060671 Male-body	SRR060672 Female-body	SRR060673 Ovary	SRR060674 Ovary	SRR060675 Ovary	SRR060676 Ovary	SRR060677 Ovary	SRR060678 Testis	SRR060679 Testis	SRR060680 Testis	SRR060681 Testis	SRR060682 Embryo	SRR060683 Testis	SRR060684 Embryo	SRR060685 Embryo	SRR060686 Embryo	SRR060687 Embryo	SRR060688 Ovary	SRR060689 Testis
.....................................................................................................................GAGCGAGCAGAGAAGTATTAT........................................................................................................................	21	1	30289.00	30289	340	0	1	6	0	10	0	9	735	2	2310	53	3259	12	0	1	1	2	0	0	0	3	0	1069	6	1	8	7	6	9	9	6361	6120	1793	6861	1	624	6	1	3	2	7	651
.....................................................................................................................GAGCGAGCAGAGAAGTATTA.........................................................................................................................	20	1	3985.00	3985	76	0	0	1	0	1	0	4	65	0	61	2	183	0	0	0	0	0	0	0	0	0	0	167	0	0	1	1	1	1	3	773	943	318	1128	0	134	0	0	0	0	1	121
.....................................................................................................................GAGCGAGCAGAGAAGTATT..........................................................................................................................	19	1	2778.00	2778	36	1	0	0	0	2	0	2	69	1	987	8	417	2	0	0	0	1	0	0	0	0	0	68	0	0	4	2	1	4	3	328	369	86	297	0	50	0	0	0	0	0	40
.....................................................................................................................GAGCGAGCAGAGAAGTATTATTT......................................................................................................................	23	1	1644.00	1644	23	0	0	0	1	0	0	0	43	0	193	1	126	1	0	0	0	0	0	0	1	1	1	63	0	1	0	0	0	0	2	391	318	84	318	0	37	0	0	0	0	0	39
.....................................................................................................................GAGCGAGCAGAGAAGTATTATT.......................................................................................................................	22	1	1011.00	1011	18	1	0	0	0	1	0	2	14	0	185	2	113	0	0	0	0	1	2	0	0	0	0	36	0	0	2	0	0	1	0	222	158	41	180	0	13	0	0	0	0	0	19
.....................................................................................................................GAGCGAGCAGAGAAGTAT...........................................................................................................................	18	1	322.00	322	0	0	0	0	0	0	0	1	10	0	118	5	48	1	0	0	0	0	0	0	0	0	0	4	0	0	2	1	2	1	0	38	51	9	28	0	2	0	0	0	0	0	1
......................................................................................................................AGCGAGCAGAGAAGTATTAT........................................................................................................................	20	1	32.00	32	1	0	0	0	0	0	0	0	2	0	3	0	4	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	4	4	0	8	0	1	0	0	0	0	0	3
........................................................................................................................CGAGCAGAGAAGTATTAT........................................................................................................................	18	1	23.00	23	0	0	0	0	0	1	0	0	0	0	4	0	0	0	0	0	0	0	1	0	0	0	0	1	0	0	0	0	0	0	0	1	11	2	2	0	0	0	0	0	0	0	0
.......................................................................................................................GCGAGCAGAGAAGTATTAT........................................................................................................................	19	1	17.00	17	0	0	0	0	0	0	0	0	0	0	3	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	3	5	1	3	0	0	0	0	0	0	0	0
.........................................................................................................................GAGCAGAGAAGTATTATTT......................................................................................................................	19	1	6.00	6	0	0	0	0	0	0	0	0	0	0	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	0	0	0	0	0	0	0	1
......................................................................................................................AGCGAGCAGAGAAGTATTA.........................................................................................................................	19	1	5.00	5	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	1	0	0	0	0	0	0	0	0
.................................................................................................ATTAACTGGGCATTCCCTCT.............................................................................................................................................	20	1	5.00	5	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	2	0	0	0	0	0	0	0	0
.........................................................................................................................GAGCAGAGAAGTATTATT.......................................................................................................................	18	1	4.00	4	0	0	0	0	0	0	0	0	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	1	0	0	0	0	0	0	0	0
...........................................................................................................................................TGCGTGAATC--TGA--TATCAAA...............................................................................................	20	1	4.00	4	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0
......................................................................................................................AGCGAGCAGAGAAGTATT..........................................................................................................................	18	1	3.00	3	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	0	0	0	0	0	0	0	0	0
..................................................................................................TTAACTGGGCATTCCCTCT.............................................................................................................................................	19	1	2.00	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	1
.......................................................................................................................GCGAGCAGAGAAGTATTATT.......................................................................................................................	20	1	2.00	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0

dsi_68

X:15011455-15011517 +

Known Ortholog

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-33.1	-32.6	-32.5