dsi_6

GCTAAGTAATAATTGAGTTGGAGCTCGACTTTTTGTAGTGAAGTATTCCCTCAAGGTCTAGAAAGTAGAGCTCAGTAATTTTACAAATTTGGCAATGCATTCAAGACCTGTGCGGAGTTATTTACACGTCGTCCATCAAAGTGTTTTGTTTTGCTGGTTTAAAA *********************************..(((((..(((((....((((((.(((.(((..((.(((.............)))))..))).))).))))))....)))))..)))))....*********************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M023 head	M024 male body	SRR553486 Ovary	SRR553485 Ovary	SRR618934 Ovary	M025 embryo	O001 Testis	M053 Female-body	SRR553487 Ovary	SRR553488 Ovary	O002 Head	GSM343915 embryo
..................................................TCAAGGTCTAGAAAGTAGAGCT............................................................................................	22	0	1	4679.00	4679	1571	1234	724	618	233	254	0	17	14	11	0	3
..................................................TCAAGGTCTAGAAAGTAGAGC.............................................................................................	21	0	1	809.00	809	225	158	177	173	48	6	18	1	1	1	1	0
..................................................TCAAGGTCTAGAAAGTAGAGCC............................................................................................	22	1	1	229.00	229	135	74	0	0	0	20	0	0	0	0	0	0
.......................................................GTCTAGAAAGTAGAGCT............................................................................................	17	0	1	108.00	108	0	0	0	0	108	0	0	0	0	0	0	0
..................................................TCAAGGTCTAGAAAGTAGAG..............................................................................................	20	0	1	93.00	93	5	4	28	30	19	0	6	0	0	0	1	0
..................................................TCAAGGTCTAGAAAGTAGAGCG............................................................................................	22	1	1	57.00	57	16	35	0	0	0	6	0	0	0	0	0	0
..................................................TCAAGGTCTAGAAAGTAGAGCTT...........................................................................................	23	1	1	33.00	33	14	10	3	4	2	0	0	0	0	0	0	0
........................................................TCTAGAAAGTAGAGCT............................................................................................	16	0	1	26.00	26	0	0	0	0	26	0	0	0	0	0	0	0
..................................................TCAAGGTCTAGAAAGTAGA...............................................................................................	19	0	1	23.00	23	0	0	7	4	5	0	7	0	0	0	0	0
..................................................TCAAGGTCTAGAAAGTAGAGCTC...........................................................................................	23	0	1	21.00	21	0	0	0	0	1	0	19	0	0	0	1	0
............................................................................................CAATGCATTCAAGACCTGTGCG..................................................	22	0	1	17.00	17	0	0	1	1	15	0	0	0	0	0	0	0
...................................................CAAGGTCTAGAAAGTAGAGCT............................................................................................	21	0	1	14.00	14	5	2	3	3	0	0	0	1	0	0	0	0
............................................................................................CAATGCATTCAAGACCTGTGC...................................................	21	0	1	11.00	11	3	2	1	0	3	0	2	0	0	0	0	0
..................................................TCAAGGTCTAGAAAGAAGAGCT............................................................................................	22	1	1	10.00	10	7	3	0	0	0	0	0	0	0	0	0	0
............................................................................................CAATGCATTCAAGACCTGTGCGT.................................................	23	1	1	10.00	10	1	0	0	0	9	0	0	0	0	0	0	0
....................................................AAGGTCTAGAAAGTAGAGCT............................................................................................	20	0	1	8.00	8	4	0	1	1	2	0	0	0	0	0	0	0
..................................................TCAAGGGCTAGAAAGTAGAGCT............................................................................................	22	1	1	8.00	8	2	6	0	0	0	0	0	0	0	0	0	0
..................................................TCAAGGTCTAGAAGGTAGAGCT............................................................................................	22	1	1	7.00	7	1	2	1	1	1	1	0	0	0	0	0	0
..................................................TCAAGGTCTAGAAAGGAGAGCT............................................................................................	22	1	1	5.00	5	3	2	0	0	0	0	0	0	0	0	0	0
..................................................TCAAGGTCTAGAAAGTATAGCT............................................................................................	22	1	1	5.00	5	0	2	1	0	0	2	0	0	0	0	0	0
.......................................................GTCTAGAAAGTAGAGC.............................................................................................	16	0	1	5.00	5	0	0	0	0	5	0	0	0	0	0	0	0
......................................................GGTCTAGAAAGTAGAGCT............................................................................................	18	0	1	5.00	5	0	0	0	0	5	0	0	0	0	0	0	0
..................................................TCAAGGTCTATAAAGTAGAGCT............................................................................................	22	1	1	5.00	5	4	0	0	0	0	1	0	0	0	0	0	0
..................................................TCAAGGCCTAGAAAGTAGAGCT............................................................................................	22	1	1	5.00	5	3	2	0	0	0	0	0	0	0	0	0	0
..................................................TCAAGGTCTAGAAAGTAGGGCT............................................................................................	22	1	1	4.00	4	2	1	0	0	0	1	0	0	0	0	0	0
..................................................CCAAGGTCTAGAAAGTAGAGCT............................................................................................	22	1	1	4.00	4	1	0	1	1	0	1	0	0	0	0	0	0
..................................................TCAAGGTCTGGAAAGTAGAGCT............................................................................................	22	1	1	4.00	4	4	0	0	0	0	0	0	0	0	0	0	0
..................................................TCAAGGTCTAAAAAGTAGAGCT............................................................................................	22	1	1	4.00	4	1	0	0	0	1	2	0	0	0	0	0	0
..................................................GCAAGGTCTAGAAAGTAGAGCT............................................................................................	22	1	1	4.00	4	0	0	4	0	0	0	0	0	0	0	0	0
..................................................TCGAGGTCTAGAAAGTAGAGCT............................................................................................	22	1	1	4.00	4	1	0	2	0	1	0	0	0	0	0	0	0
..................................................ACAAGGTCTAGAAAGTAGAGCT............................................................................................	22	1	1	4.00	4	0	0	1	3	0	0	0	0	0	0	0	0
..................................................TAAAGGTCTAGAAAGTAGAGCT............................................................................................	22	1	1	4.00	4	1	0	0	1	2	0	0	0	0	0	0	0
..................................................TCAATGTCTAGAAAGTAGAGCT............................................................................................	22	1	1	4.00	4	2	0	0	0	0	2	0	0	0	0	0	0
..................................................TCAAGGTCTAGAAAGCAGAGCT............................................................................................	22	1	1	3.00	3	2	1	0	0	0	0	0	0	0	0	0	0
..................................................TTAAGGTCTAGAAAGTAGAGCT............................................................................................	22	1	1	3.00	3	0	0	0	0	2	1	0	0	0	0	0	0
..................................................TCAAGGTCTATAAAGTAGAGC.............................................................................................	21	1	1	3.00	3	1	1	0	0	0	1	0	0	0	0	0	0
..................................................TCAGGGTCTAGAAAGTAGAGCT............................................................................................	22	1	1	3.00	3	1	0	0	1	0	1	0	0	0	0	0	0
............................................................................................CAATGCATTCAAGACCTGT.....................................................	19	0	1	3.00	3	0	0	0	0	1	0	2	0	0	0	0	0
..................................................TCAAGGTATAGAAAGTAGAGCT............................................................................................	22	1	1	2.00	2	0	2	0	0	0	0	0	0	0	0	0	0
..................................................TTAAGGTCTAGAAAGTAGAGC.............................................................................................	21	1	1	2.00	2	0	1	0	1	0	0	0	0	0	0	0	0
......................................................GGTCTAGAAAGTAGAGC.............................................................................................	17	0	1	2.00	2	0	0	0	0	2	0	0	0	0	0	0	0
............................................................................................CAATGCATTTAAGACCTGTGCG..................................................	22	1	1	2.00	2	0	0	2	0	0	0	0	0	0	0	0	0
..................................................TCAAGGTCTACAAAGTAGAGCT............................................................................................	22	1	1	2.00	2	0	1	0	0	0	1	0	0	0	0	0	0
..................................................TCAAGGTCTAGAAAGT..................................................................................................	16	0	1	2.00	2	0	0	1	0	1	0	0	0	0	0	0	0
..................................................TCAAGGTCTAGAAAGTAG................................................................................................	18	0	1	2.00	2	0	0	0	0	0	0	2	0	0	0	0	0
..................................................TCAAGGTCCAGAAAGTAGAGCT............................................................................................	22	1	1	2.00	2	0	0	1	0	1	0	0	0	0	0	0	0
..................................................TCAAAGTCTAGAAAGTAGAGC.............................................................................................	21	1	1	2.00	2	2	0	0	0	0	0	0	0	0	0	0	0
...................................................CAAGGTCTAGAAAGTAGAGC.............................................................................................	20	0	1	2.00	2	2	0	0	0	0	0	0	0	0	0	0	0
..................................................TCAAGGTCTAGAAAGTGGAG..............................................................................................	20	1	1	2.00	2	0	0	1	0	1	0	0	0	0	0	0	0
..................................................TCAAGGGCTAGAAAGTAGAGCC............................................................................................	22	2	1	2.00	2	1	0	0	0	0	1	0	0	0	0	0	0
..................................................TCAAGGTCTAGAAAGTAGAGAT............................................................................................	22	1	1	2.00	2	1	0	1	0	0	0	0	0	0	0	0	0
............................................................................................CAATGCATTCAAGACCTGTGCT..................................................	22	1	1	2.00	2	0	1	0	1	0	0	0	0	0	0	0	0
..................................................TCAAGGTCTAGAAAGTATAGCC............................................................................................	22	2	1	2.00	2	2	0	0	0	0	0	0	0	0	0	0	0
..................................................TCAAGCTCTAGAAAGTAGAGCT............................................................................................	22	1	1	2.00	2	1	1	0	0	0	0	0	0	0	0	0	0
............................................................................................CAATGCATTCAAGACCTGTG....................................................	20	0	1	2.00	2	0	0	0	1	1	0	0	0	0	0	0	0
..................................................TCAAGTTCTAGAAAGTAGAGCT............................................................................................	22	1	1	2.00	2	0	1	0	0	0	1	0	0	0	0	0	0
..................................................TCAAGGTCTAGAAAGTAGAGCTTT..........................................................................................	24	2	1	2.00	2	0	2	0	0	0	0	0	0	0	0	0	0
..................................................TCAACGTCTAGAAAGTAGAGCT............................................................................................	22	1	1	2.00	2	2	0	0	0	0	0	0	0	0	0	0	0
..................................................TCAAGGTCTAGAAAGTAGAAC.............................................................................................	21	1	1	2.00	2	2	0	0	0	0	0	0	0	0	0	0	0
............................................................................................CAATGCATTCAAGACCTG......................................................	18	0	1	2.00	2	1	0	0	0	0	0	1	0	0	0	0	0
..................................................TGAAGGTCTAGAAAGTAGAGCT............................................................................................	22	1	1	2.00	2	0	0	0	1	1	0	0	0	0	0	0	0
................................................................................................GCATTCAAGACCTGTGCGT.................................................	19	1	1	2.00	2	0	0	0	0	2	0	0	0	0	0	0	0
..................................................TCAAGGTCTAGAAATTAGAGCT............................................................................................	22	1	1	2.00	2	1	0	1	0	0	0	0	0	0	0	0	0
.......................................................GTCTAGAAAGTAGAG..............................................................................................	15	0	1	2.00	2	0	0	0	0	2	0	0	0	0	0	0	0
............................................................................................CAATGCATTCAAGACC........................................................	16	0	1	2.00	2	0	0	0	0	2	0	0	0	0	0	0	0
........................................................TCTAGAAAGTAGAGC.............................................................................................	15	0	1	2.00	2	0	0	0	0	2	0	0	0	0	0	0	0
.........................................................CTAGAAAGTAGAGCT............................................................................................	15	0	3	1.33	4	0	0	0	0	4	0	0	0	0	0	0	0
..................................................TCAAGATCCAGAAAGTAGAGC.............................................................................................	21	2	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0
..................................................TCAAGGTCTAGAAAGTAGAGAG............................................................................................	22	2	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0
............................................................................................CAATGCATTCAAGACCTGTGTT..................................................	22	2	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
..................................................TCAAGGTCTAGAAAGTAGAGCA............................................................................................	22	1	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
..................................................TCAAGGTCTAGAAAGTAGAACT............................................................................................	22	1	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
.......................................................GTCTGGAAAGTAGAGCT............................................................................................	17	1	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
..................................................TCAAGGTCTTGAAAGTAGAGCT............................................................................................	22	1	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0
..................................................TCAAGGTCTAGAAAGCAGAGC.............................................................................................	21	1	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0
............................................................................................CAATGCATTCAAGACCTGTGCGA.................................................	23	1	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
.............................................................................................AATGCATTCAAGACCTGTGCGT.................................................	22	1	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
..................................................TCAAGGTCTAGAAAGTAGAGGT............................................................................................	22	1	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0
..................................................TCAAGGTCTGGAAAGTAGAG..............................................................................................	20	1	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0
..................................................TCAAGGTCTAGAAGGTAGAGC.............................................................................................	21	1	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0
..................................................TCAAGGTCTAGAAAGTA.................................................................................................	17	0	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0
.....................................................AGGTCTAGAAAGTAGAGCT............................................................................................	19	0	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
..................................................TCAAGGTCTAGAAATTAGAGC.............................................................................................	21	1	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
..................................................TCAAGGTCTAGAAACTAGAGCT............................................................................................	22	1	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0
..................................................TCAAGGTCTAGAAAGTAGCGCT............................................................................................	22	1	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0
..................................................TCAAGGTCTAGAAAGAAGAGCG............................................................................................	22	2	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0
..................................................TCAAGGTCTAGAAAGTAGCT..............................................................................................	20	2	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
.................................................................................................CATTCAAGACCTGTGCGT.................................................	18	1	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
..................................................TCAAGGTCTAGAAAGTAGAGCAT...........................................................................................	23	2	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0
..................................................TGAAGGTCTAGAAAGTAGAGC.............................................................................................	21	1	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
..........................................................................................................................................AAATGCTTTGTTTTGCTGGT......	20	2	1	1.00	1	0	0	0	0	0	0	0	0	1	0	0	0
..................................................TCAAGGTCTAGAAAATAGAGCT............................................................................................	22	1	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0
..................................................TCAAGGCCTAGAAAGTAGAGCC............................................................................................	22	2	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0
..................................................TCAAGGTCTAGAAAGTACAGC.............................................................................................	21	1	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0
................................................................................................GCATTCAAGACCTGTGCG..................................................	18	0	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
..................................................ACAGGGTCTAGAAAGTAGAGCT............................................................................................	22	2	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0
..................................................TCAAGGTCTAGAAAGTAGAGCTTGA.........................................................................................	25	3	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
..................................................TCAAGGTCTAGTAAGTAGAGC.............................................................................................	21	1	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0
..................................................TCAAGGTCTCGAAAGTAGAGCT............................................................................................	22	1	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0
....................................................AAGGTCTAGAAAGCAGAGCT............................................................................................	20	1	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
........................................................TCTAGAAAGTAGAGCG............................................................................................	16	1	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
..................................................TCAAGGTCTAGAAAGAAGAG..............................................................................................	20	1	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
...................................................CAATGTCTAGAAAGTAGAGCT............................................................................................	21	1	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0
............................................................................................CAATGCATTCAAGACCTGTGCGG.................................................	23	0	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0
..................................................TCAAGGTCTATAAAGTAGAGCC............................................................................................	22	2	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0
..................................................TCAAGGTCTAGAAAGTAGAGT.............................................................................................	21	1	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0
....................................................GAGCTCTAGAAAGTAGAGCT............................................................................................	20	2	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
..................................................TCAAGGTCTAGAAAGCAGAGCC............................................................................................	22	2	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0
..................................................TCAAGGTCTAGAAAGTACAGCC............................................................................................	22	2	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0
..................................................TCAAGGTCTAGAAAGTAGATTT............................................................................................	22	2	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	1
..................................................TCAAGGTCTAGAAAGAAGAGC.............................................................................................	21	1	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0
.......................................................................................TTTGGCAATGCATTCAAGACCTGTGC...................................................	26	0	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0
..................................................TCACGGTCTAGAAAGTAGAGCT............................................................................................	22	1	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0
..................................................TCAAGGTCTAGGAAGTAGAGCT............................................................................................	22	1	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
..................................................TCAAGATCTAGAAAGTAGAGC.............................................................................................	21	1	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0
..................................................TCAAGGTTTAGAAAGTAGAGCT............................................................................................	22	1	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0
..................................................TCAAGGACTAGAAAGTAGAGCT............................................................................................	22	1	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0
....................................................AAGGTCTAGAAAGTAGAGC.............................................................................................	19	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0
..............................................TCCCTCATGGTCTAG.......................................................................................................	15	1	3	0.33	1	0	0	1	0	0	0	0	0	0	0	0	0
...................................................TCATGTCTAGAAAGTAGAGCT............................................................................................	21	3	3	0.33	1	0	0	0	0	0	1	0	0	0	0	0	0
.............TGAATTGGAGCTCGAC.......................................................................................................................................	16	1	3	0.33	1	0	0	0	1	0	0	0	0	0	0	0	0
............TTGAATTGGAGCTCGACC......................................................................................................................................	18	2	8	0.13	1	0	0	1	0	0	0	0	0	0	0	0	0
....................................................ATTCTCTAGAAAGTAGAGCT............................................................................................	20	3	10	0.10	1	0	0	0	0	1	0	0	0	0	0	0	0
..................................................TCAAGGTCTAGAAAA...................................................................................................	15	1	12	0.08	1	0	0	0	1	0	0	0	0	0	0	0	0
........ATAATTGAGTGGGAGTACGA........................................................................................................................................	20	3	12	0.08	1	0	0	0	0	0	0	0	0	0	1	0	0
............TTGAATCGGAGCTCGAC.......................................................................................................................................	17	2	14	0.07	1	0	0	0	0	1	0	0	0	0	0	0	0
.............TGAATTGGAGCTCGACC......................................................................................................................................	17	2	16	0.06	1	0	0	1	0	0	0	0	0	0	0	0	0
..............................................................................................................................................GTTTTGTTTTGCTGG.......	15	0	18	0.06	1	0	0	0	0	1	0	0	0	0	0	0	0

GCTAAGTAATAATTGAGTTGGAGCTCGACTTTTTGTAGTGAAGTATTCCCTCAAGGTCTAGAAAGTAGAGCTCAGTAATTTTACAAATTTGGCAATGCATTCAAGACCTGTGCGGAGTTATTTACACGTCGTCCATCAAAGTGTTTTGTTTTGCTGGTTTAAAA
***********************************..(((((..(((((....((((((.(((.(((..((.(((.............)))))..))).))).))))))....)))))..)))))....***********************************

Read size

# Mismatch

Hit Count

Total Norm

Total

M023
head

M024
male body

SRR553486
Ovary

SRR553485
Ovary

SRR618934
Ovary

M025
embryo

O001
Testis

M053
Female-body

SRR553487
Ovary

SRR553488
Ovary

O002
Head

GSM343915
embryo

..................................................TCAAGGTCTAGAAAGTAGAGCT............................................................................................

4679.00

4679

1571

1234

724

618

233

254

..................................................TCAAGGTCTAGAAAGTAGAGC.............................................................................................

809.00

809

225

158

177

173

..................................................TCAAGGTCTAGAAAGTAGAGCC............................................................................................

229.00

229

135

.......................................................GTCTAGAAAGTAGAGCT............................................................................................

108.00

108

..................................................TCAAGGTCTAGAAAGTAGAG..............................................................................................

93.00

..................................................TCAAGGTCTAGAAAGTAGAGCG............................................................................................

57.00

..................................................TCAAGGTCTAGAAAGTAGAGCTT...........................................................................................

33.00

........................................................TCTAGAAAGTAGAGCT............................................................................................

26.00

..................................................TCAAGGTCTAGAAAGTAGA...............................................................................................

23.00

..................................................TCAAGGTCTAGAAAGTAGAGCTC...........................................................................................

21.00

............................................................................................CAATGCATTCAAGACCTGTGCG..................................................

17.00

...................................................CAAGGTCTAGAAAGTAGAGCT............................................................................................

14.00

............................................................................................CAATGCATTCAAGACCTGTGC...................................................

11.00

..................................................TCAAGGTCTAGAAAGAAGAGCT............................................................................................

10.00

............................................................................................CAATGCATTCAAGACCTGTGCGT.................................................

10.00

....................................................AAGGTCTAGAAAGTAGAGCT............................................................................................

8.00

..................................................TCAAGGGCTAGAAAGTAGAGCT............................................................................................

8.00

..................................................TCAAGGTCTAGAAGGTAGAGCT............................................................................................

7.00

..................................................TCAAGGTCTAGAAAGGAGAGCT............................................................................................

5.00

..................................................TCAAGGTCTAGAAAGTATAGCT............................................................................................

5.00

.......................................................GTCTAGAAAGTAGAGC.............................................................................................

5.00

......................................................GGTCTAGAAAGTAGAGCT............................................................................................

5.00

..................................................TCAAGGTCTATAAAGTAGAGCT............................................................................................

5.00

..................................................TCAAGGCCTAGAAAGTAGAGCT............................................................................................

5.00

..................................................TCAAGGTCTAGAAAGTAGGGCT............................................................................................

4.00

..................................................CCAAGGTCTAGAAAGTAGAGCT............................................................................................

4.00

..................................................TCAAGGTCTGGAAAGTAGAGCT............................................................................................

4.00

..................................................TCAAGGTCTAAAAAGTAGAGCT............................................................................................

4.00

..................................................GCAAGGTCTAGAAAGTAGAGCT............................................................................................

4.00

..................................................TCGAGGTCTAGAAAGTAGAGCT............................................................................................

4.00

..................................................ACAAGGTCTAGAAAGTAGAGCT............................................................................................

4.00

..................................................TAAAGGTCTAGAAAGTAGAGCT............................................................................................

4.00

..................................................TCAATGTCTAGAAAGTAGAGCT............................................................................................

4.00

..................................................TCAAGGTCTAGAAAGCAGAGCT............................................................................................

3.00

..................................................TTAAGGTCTAGAAAGTAGAGCT............................................................................................

3.00

..................................................TCAAGGTCTATAAAGTAGAGC.............................................................................................

3.00

..................................................TCAGGGTCTAGAAAGTAGAGCT............................................................................................

3.00

............................................................................................CAATGCATTCAAGACCTGT.....................................................

3.00

..................................................TCAAGGTATAGAAAGTAGAGCT............................................................................................

2.00

..................................................TTAAGGTCTAGAAAGTAGAGC.............................................................................................

2.00

......................................................GGTCTAGAAAGTAGAGC.............................................................................................

2.00

............................................................................................CAATGCATTTAAGACCTGTGCG..................................................

2.00

..................................................TCAAGGTCTACAAAGTAGAGCT............................................................................................

2.00

..................................................TCAAGGTCTAGAAAGT..................................................................................................

2.00

..................................................TCAAGGTCTAGAAAGTAG................................................................................................

2.00

..................................................TCAAGGTCCAGAAAGTAGAGCT............................................................................................

2.00

..................................................TCAAAGTCTAGAAAGTAGAGC.............................................................................................

2.00

...................................................CAAGGTCTAGAAAGTAGAGC.............................................................................................

2.00

..................................................TCAAGGTCTAGAAAGTGGAG..............................................................................................

2.00

..................................................TCAAGGGCTAGAAAGTAGAGCC............................................................................................

2.00

..................................................TCAAGGTCTAGAAAGTAGAGAT............................................................................................

2.00

............................................................................................CAATGCATTCAAGACCTGTGCT..................................................

2.00

..................................................TCAAGGTCTAGAAAGTATAGCC............................................................................................

2.00

..................................................TCAAGCTCTAGAAAGTAGAGCT............................................................................................

2.00

............................................................................................CAATGCATTCAAGACCTGTG....................................................

2.00

..................................................TCAAGTTCTAGAAAGTAGAGCT............................................................................................

2.00

..................................................TCAAGGTCTAGAAAGTAGAGCTTT..........................................................................................

2.00

..................................................TCAACGTCTAGAAAGTAGAGCT............................................................................................

2.00

..................................................TCAAGGTCTAGAAAGTAGAAC.............................................................................................

2.00

............................................................................................CAATGCATTCAAGACCTG......................................................

2.00

..................................................TGAAGGTCTAGAAAGTAGAGCT............................................................................................

2.00

................................................................................................GCATTCAAGACCTGTGCGT.................................................

2.00

..................................................TCAAGGTCTAGAAATTAGAGCT............................................................................................

2.00

.......................................................GTCTAGAAAGTAGAG..............................................................................................

2.00

............................................................................................CAATGCATTCAAGACC........................................................

2.00

........................................................TCTAGAAAGTAGAGC.............................................................................................

2.00

.........................................................CTAGAAAGTAGAGCT............................................................................................

1.33

..................................................TCAAGATCCAGAAAGTAGAGC.............................................................................................

1.00

..................................................TCAAGGTCTAGAAAGTAGAGAG............................................................................................

1.00

............................................................................................CAATGCATTCAAGACCTGTGTT..................................................

1.00

..................................................TCAAGGTCTAGAAAGTAGAGCA............................................................................................

1.00

..................................................TCAAGGTCTAGAAAGTAGAACT............................................................................................

1.00

.......................................................GTCTGGAAAGTAGAGCT............................................................................................

1.00

..................................................TCAAGGTCTTGAAAGTAGAGCT............................................................................................

1.00

..................................................TCAAGGTCTAGAAAGCAGAGC.............................................................................................

1.00

............................................................................................CAATGCATTCAAGACCTGTGCGA.................................................

1.00

.............................................................................................AATGCATTCAAGACCTGTGCGT.................................................

1.00

..................................................TCAAGGTCTAGAAAGTAGAGGT............................................................................................

1.00

..................................................TCAAGGTCTGGAAAGTAGAG..............................................................................................

1.00

..................................................TCAAGGTCTAGAAGGTAGAGC.............................................................................................

1.00

..................................................TCAAGGTCTAGAAAGTA.................................................................................................

1.00

.....................................................AGGTCTAGAAAGTAGAGCT............................................................................................

1.00

..................................................TCAAGGTCTAGAAATTAGAGC.............................................................................................

1.00

..................................................TCAAGGTCTAGAAACTAGAGCT............................................................................................

1.00

..................................................TCAAGGTCTAGAAAGTAGCGCT............................................................................................

1.00

..................................................TCAAGGTCTAGAAAGAAGAGCG............................................................................................

1.00

..................................................TCAAGGTCTAGAAAGTAGCT..............................................................................................

1.00

.................................................................................................CATTCAAGACCTGTGCGT.................................................

1.00

..................................................TCAAGGTCTAGAAAGTAGAGCAT...........................................................................................

1.00

..................................................TGAAGGTCTAGAAAGTAGAGC.............................................................................................

1.00

..........................................................................................................................................AAATGCTTTGTTTTGCTGGT......

1.00

..................................................TCAAGGTCTAGAAAATAGAGCT............................................................................................

1.00

..................................................TCAAGGCCTAGAAAGTAGAGCC............................................................................................

1.00

..................................................TCAAGGTCTAGAAAGTACAGC.............................................................................................

1.00

................................................................................................GCATTCAAGACCTGTGCG..................................................

1.00

..................................................ACAGGGTCTAGAAAGTAGAGCT............................................................................................

1.00

..................................................TCAAGGTCTAGAAAGTAGAGCTTGA.........................................................................................

1.00

..................................................TCAAGGTCTAGTAAGTAGAGC.............................................................................................

1.00

..................................................TCAAGGTCTCGAAAGTAGAGCT............................................................................................

1.00

....................................................AAGGTCTAGAAAGCAGAGCT............................................................................................

1.00

........................................................TCTAGAAAGTAGAGCG............................................................................................

1.00

..................................................TCAAGGTCTAGAAAGAAGAG..............................................................................................

1.00

...................................................CAATGTCTAGAAAGTAGAGCT............................................................................................

1.00

............................................................................................CAATGCATTCAAGACCTGTGCGG.................................................

1.00

..................................................TCAAGGTCTATAAAGTAGAGCC............................................................................................

1.00

..................................................TCAAGGTCTAGAAAGTAGAGT.............................................................................................

1.00

....................................................GAGCTCTAGAAAGTAGAGCT............................................................................................

1.00

..................................................TCAAGGTCTAGAAAGCAGAGCC............................................................................................

1.00

..................................................TCAAGGTCTAGAAAGTACAGCC............................................................................................

1.00

..................................................TCAAGGTCTAGAAAGTAGATTT............................................................................................

1.00

..................................................TCAAGGTCTAGAAAGAAGAGC.............................................................................................

1.00

.......................................................................................TTTGGCAATGCATTCAAGACCTGTGC...................................................

1.00

..................................................TCACGGTCTAGAAAGTAGAGCT............................................................................................

1.00

..................................................TCAAGGTCTAGGAAGTAGAGCT............................................................................................

1.00

..................................................TCAAGATCTAGAAAGTAGAGC.............................................................................................

1.00

..................................................TCAAGGTTTAGAAAGTAGAGCT............................................................................................

1.00

..................................................TCAAGGACTAGAAAGTAGAGCT............................................................................................

1.00

....................................................AAGGTCTAGAAAGTAGAGC.............................................................................................

1.00

..............................................TCCCTCATGGTCTAG.......................................................................................................

0.33

...................................................TCATGTCTAGAAAGTAGAGCT............................................................................................

0.33

.............TGAATTGGAGCTCGAC.......................................................................................................................................

0.33

............TTGAATTGGAGCTCGACC......................................................................................................................................

0.13

....................................................ATTCTCTAGAAAGTAGAGCT............................................................................................

0.10

..................................................TCAAGGTCTAGAAAA...................................................................................................

0.08

........ATAATTGAGTGGGAGTACGA........................................................................................................................................

0.08

............TTGAATCGGAGCTCGAC.......................................................................................................................................

0.07

.............TGAATTGGAGCTCGACC......................................................................................................................................

0.06

..............................................................................................................................................GTTTTGTTTTGCTGG.......

0.06

TTTTAAACCAGCAAAACAAAACACTTTGATGGACGACGTGTAAATAACTCCGCACAGGTCTTGAATGCATTGCCAAATTTGTAAAATTACTGAGCTCTACTTTCTAGACCTTGAGGGAATACTTCACTACAAAAAGTCGAGCTCCAACTCAATTATTACTTAGC *********************************....(((((..(((((....((((((.(((.(((..(((((.............))).))..))).))).))))))....)))))..)))))..*********************************	Read size	# Mismatch	Hit Count	Total Norm	Total
..................................GACGTGCAAATAACTC..................................................................................................................	16	1	4	0.25	1
..........................................................................................................................TTCACTACAAAAAGACTAGG......................	20	3	7	0.14	1
..........................................................TCTTGAATGCAAAGCAAAATT.....................................................................................	21	3	14	0.07	1
.................................CGACGTGTAAAAAAC....................................................................................................................	15	1	16	0.06	1
................TAAAACAATTTGATGGAC..................................................................................................................................	18	2	20	0.05	1
...........................GATGAAGGACGTGTAA.........................................................................................................................	16	2	20	0.05	1

TTTTAAACCAGCAAAACAAAACACTTTGATGGACGACGTGTAAATAACTCCGCACAGGTCTTGAATGCATTGCCAAATTTGTAAAATTACTGAGCTCTACTTTCTAGACCTTGAGGGAATACTTCACTACAAAAAGTCGAGCTCCAACTCAATTATTACTTAGC
***********************************....(((((..(((((....((((((.(((.(((..(((((.............))).))..))).))).))))))....)))))..)))))..***********************************

Read size

# Mismatch

Hit Count

Total Norm

Total

..................................GACGTGCAAATAACTC..................................................................................................................

0.25

..........................................................................................................................TTCACTACAAAAAGACTAGG......................

0.14

..........................................................TCTTGAATGCAAAGCAAAATT.....................................................................................

0.07

.................................CGACGTGTAAAAAAC....................................................................................................................

0.06

................TAAAACAATTTGATGGAC..................................................................................................................................

0.05

...........................GATGAAGGACGTGTAA.........................................................................................................................

0.05

Species	Coordinate	ID	Alignment
droSim3	X:16526618-16526781 -	dsi_6	GCTAAGTAA---TAA-TTGAGT-TGGAGC-TCGACTTTTTGTAGTGAAGTATT--------CCCTCAAGGTCTAGAAAGTAGAGCTCAGTAATTTTACAAATTTGGCAATGCATTCAAGACCTGT-GCGGAGTTATTTACACGTCGTCC---ATCAA-----------AGTGTTTTGTTTTGCTGGTTTAA-AA--------------
droSec2	scaffold_8:3725797-3725960 -	dse_127	GCTAAGTAA---TAA-TTGAGT-TGGAGC-TCGACTTTTTGTAGTGAAGTATT--------CCCTCAAGGTCTAGAAAGTAGAGCTCAGTAATTTTACAAATTTGGCAATGCATTCAAGACCTGT-GCGGAGTTATTTACACGTCGTCC---ATCAA-----------AGTGTTTTGTTTTGCTGGTTTGA-AA--------------
dm3	chrX:21443075-21443242 -		GCTAAGTGA---TAA-TTGAATCTG-----GAAATAA--TAGAATGGAAGAAAAATGGAAATGATAATAATCTGGAAAGTAGAGCTCAGTAATTTTACAAATTTGGCAATGCCTTCAAGACCTGT-GCGGAGTTTTTTACACGTCTTCC---ATTAA-----------AGTGTTTTGGTTTGTTTGTTTGATAA--------------
droEre2	scaffold_4690:17924532-17924676 -	der_47	GCTGAGA-A---TTA-TTGCCT-GGAACC-TCGAGAT--TGTTGTGAA--ATT--------CCCTTAAGGTCAGGAAAGTATAGCTCAGTGATTT-GCGAATTTAGCAATACATTCTAGACCTTT-GCGGAGTTTTTCAAAGGTCTTCC---GTGAA-----------AGTGTTCTGCTT----------------------------
droYak3	X:20580767-20580915 -	dya_40	GCTAAGAAA---C---------------C-AGGACGT--CTTTGTGAATTATT--------CCCTTAAGGTCAGAAATGTATAGCTTAGTGGCTTTACAAATTTGGCAATACATTTAAGACCTTA-GCGGAGTTTCTCACATGCCTTCC---ATAAA-----------AGTGTCTAGTCTAGACAATTTAA-AA--------------
droEug1	scf7180000408193:45617-45726 +		GCAAAGTAAA--TCATTTAACT-TACACT-TTCATTTCAAATTGTGA-GTATT--------TTCTTAAGGTCTGAACAGTATAGCTCAGTAACTTAAGAAATTCTGCAATACATTCAAGACTT-------------------------------------------------------------------------------------
droTak1	scf7180000415769:353326-353498 -		GGTGCGTAA---GAA-TTAAAT----------AATTT--TCTTGTGAACTTTT--------TCCTTAAGGTCTGGGAAGTATAGCTAAGTGATTTTAAATATTCTGCAATGCATTCAAGACCTTTTGCAAAG-GGTTCACACGTGTTTCTTTATTGAATTGTTTATGAAGTGT---------CTGTTTTGA-AAGAAACTTAAAATTC
droEle1	scf7180000491087:1001998-1002140 +		AAGAAGTTTT--TAATTTGTGT-T------AAAACAT--TTTTGTGAAATATT--------TTCTTAAGGTCTTGAAGATATACCTTAGTAATTTTAAAAAGTCGGTAATATATTCAAGATCTTCTGTAAAGTGTTTTTTTCATGTTTCTT-GTTAC-----------TCCCTT----------------------------------
droRho1	scf7180000774347:141-274 +		GTGAACAAAT--TAA-TTTCTT-TGGGCT-TTAACTT--TTTTGTGAAATATT--------TTCTTAAGGTCTGGAAAGTATATCAATGTATATTAAAAAATTCTGCTATATTTTAAAGACCTTTTGCAAAGTGCTTTACTCGTGATTC-----------------------------------------------------------
droFic1	scf7180000453926:397642-397776 -		GGGAATTCCTCCGAA-GTGACT-AAG-CCGCAGAAGT--GTCTGTGATATATT--------TTCTTAAGGTCTGGAAAGTATAGCAACGGCATTTTA-AACTCCTGCGATACATTCAAGACCTTT-TGTAAATTTCCTACTCGTGGTTC---A-------------------------------------------------------

Species

Coordinate

Alignment

droSim3

X:16526618-16526781 -

dsi_6

GCTAAGTAA---TAA-TTGAGT-TGGAGC-TCGACTTTTTGTAGTGAAGTATT--------CCCTCAAGGTCTAGAAAGTAGAGCTCAGTAATTTTACAAATTTGGCAATGCATTCAAGACCTGT-GCGGAGTTATTTACACGTCGTCC---ATCAA-----------AGTGTTTTGTTTTGCTGGTTTAA-AA--------------

droSec2

scaffold_8:3725797-3725960 -

dse_127

dm3

chrX:21443075-21443242 -

GCTAAGTGA---TAA-TTGAATCTG-----GAAATAA--TAGAATGGAAGAAAAATGGAAATGATAATAATCTGGAAAGTAGAGCTCAGTAATTTTACAAATTTGGCAATGCCTTCAAGACCTGT-GCGGAGTTTTTTACACGTCTTCC---ATTAA-----------AGTGTTTTGGTTTGTTTGTTTGATAA--------------

droEre2

scaffold_4690:17924532-17924676 -

der_47

GCTGAGA-A---TTA-TTGCCT-GGAACC-TCGAGAT--TGTTGTGAA--ATT--------CCCTTAAGGTCAGGAAAGTATAGCTCAGTGATTT-GCGAATTTAGCAATACATTCTAGACCTTT-GCGGAGTTTTTCAAAGGTCTTCC---GTGAA-----------AGTGTTCTGCTT----------------------------

droYak3

X:20580767-20580915 -

dya_40

GCTAAGAAA---C---------------C-AGGACGT--CTTTGTGAATTATT--------CCCTTAAGGTCAGAAATGTATAGCTTAGTGGCTTTACAAATTTGGCAATACATTTAAGACCTTA-GCGGAGTTTCTCACATGCCTTCC---ATAAA-----------AGTGTCTAGTCTAGACAATTTAA-AA--------------

droEug1

scf7180000408193:45617-45726 +

GCAAAGTAAA--TCATTTAACT-TACACT-TTCATTTCAAATTGTGA-GTATT--------TTCTTAAGGTCTGAACAGTATAGCTCAGTAACTTAAGAAATTCTGCAATACATTCAAGACTT-------------------------------------------------------------------------------------

droTak1

scf7180000415769:353326-353498 -

GGTGCGTAA---GAA-TTAAAT----------AATTT--TCTTGTGAACTTTT--------TCCTTAAGGTCTGGGAAGTATAGCTAAGTGATTTTAAATATTCTGCAATGCATTCAAGACCTTTTGCAAAG-GGTTCACACGTGTTTCTTTATTGAATTGTTTATGAAGTGT---------CTGTTTTGA-AAGAAACTTAAAATTC

droEle1

scf7180000491087:1001998-1002140 +

AAGAAGTTTT--TAATTTGTGT-T------AAAACAT--TTTTGTGAAATATT--------TTCTTAAGGTCTTGAAGATATACCTTAGTAATTTTAAAAAGTCGGTAATATATTCAAGATCTTCTGTAAAGTGTTTTTTTCATGTTTCTT-GTTAC-----------TCCCTT----------------------------------

droRho1

scf7180000774347:141-274 +

GTGAACAAAT--TAA-TTTCTT-TGGGCT-TTAACTT--TTTTGTGAAATATT--------TTCTTAAGGTCTGGAAAGTATATCAATGTATATTAAAAAATTCTGCTATATTTTAAAGACCTTTTGCAAAGTGCTTTACTCGTGATTC-----------------------------------------------------------

droFic1

scf7180000453926:397642-397776 -

GGGAATTCCTCCGAA-GTGACT-AAG-CCGCAGAAGT--GTCTGTGATATATT--------TTCTTAAGGTCTGGAAAGTATAGCAACGGCATTTTA-AACTCCTGCGATACATTCAAGACCTTT-TGTAAATTTCCTACTCGTGGTTC---A-------------------------------------------------------

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droSim3

GCTAAGTAA---TAA-TTGAGT-TGGAGC-TCGACTTTTTGTAGTGAAGTATT--------CCCTCAAGGTCTAGAAAGTAGAGCTCAGTAATTTTACAAATTTGGCAATGCATTCAAGACCTGT-GCGGAGTTATTTACACGTCGTCC---ATCAA-----------AGTGTTTTGTTTTGCTGGTTTAA-AA--------------	Size	Hit Count	Total Norm	Total	V044 Embryo	M023 Head	M024 Male-body	M025 Embryo	O001 Testis	O002 Head	M053 Female-body	SRR553485 Ovary	SRR553486 Ovary	SRR553487 Ovary	SRR553488 Ovary	SRR618934 Ovary
................................................................TCAAGGTCTAGAAAGTAGAGCT..........................................................................................................................	22	1	4679.00	4679	3	1571	1234	254	0	0	17	618	724	14	11	233
................................................................TCAAGGTCTAGAAAGTAGAGC...........................................................................................................................	21	1	809.00	809	0	225	158	6	18	1	1	173	177	1	1	48
.....................................................................GTCTAGAAAGTAGAGCT..........................................................................................................................	17	1	108.00	108	0	0	0	0	0	0	0	0	0	0	0	108
................................................................TCAAGGTCTAGAAAGTAGAG............................................................................................................................	20	1	93.00	93	0	5	4	0	6	1	0	30	28	0	0	19
......................................................................TCTAGAAAGTAGAGCT..........................................................................................................................	16	1	26.00	26	0	0	0	0	0	0	0	0	0	0	0	26
................................................................TCAAGGTCTAGAAAGTAGA.............................................................................................................................	19	1	23.00	23	0	0	0	0	7	0	0	4	7	0	0	5
................................................................TCAAGGTCTAGAAAGTAGAGCTC.........................................................................................................................	23	1	21.00	21	0	0	0	0	19	1	0	0	0	0	0	1
..........................................................................................................CAATGCATTCAAGACCTGT-GCG...............................................................................	22	1	17.00	17	0	0	0	0	0	0	0	1	1	0	0	15
.................................................................CAAGGTCTAGAAAGTAGAGCT..........................................................................................................................	21	1	14.00	14	0	5	2	0	0	0	1	3	3	0	0	0
..........................................................................................................CAATGCATTCAAGACCTGT-GC................................................................................	21	1	11.00	11	0	3	2	0	2	0	0	0	1	0	0	3
..................................................................AAGGTCTAGAAAGTAGAGCT..........................................................................................................................	20	1	8.00	8	0	4	0	0	0	0	0	1	1	0	0	2
.....................................................................GTCTAGAAAGTAGAGC...........................................................................................................................	16	1	5.00	5	0	0	0	0	0	0	0	0	0	0	0	5
....................................................................GGTCTAGAAAGTAGAGCT..........................................................................................................................	18	1	5.00	5	0	0	0	0	0	0	0	0	0	0	0	5
..........................................................................................................CAATGCATTCAAGACCTGT-..................................................................................	19	1	3.00	3	0	0	0	0	2	0	0	0	0	0	0	1
....................................................................GGTCTAGAAAGTAGAGC...........................................................................................................................	17	1	2.00	2	0	0	0	0	0	0	0	0	0	0	0	2
................................................................TCAAGGTCTAGAAAGT................................................................................................................................	16	1	2.00	2	0	0	0	0	0	0	0	0	1	0	0	1
................................................................TCAAGGTCTAGAAAGTAG..............................................................................................................................	18	1	2.00	2	0	0	0	0	2	0	0	0	0	0	0	0
.................................................................CAAGGTCTAGAAAGTAGAGC...........................................................................................................................	20	1	2.00	2	0	2	0	0	0	0	0	0	0	0	0	0
..........................................................................................................CAATGCATTCAAGACCTGT-G.................................................................................	20	1	2.00	2	0	0	0	0	0	0	0	1	0	0	0	1
..........................................................................................................CAATGCATTCAAGACCTG....................................................................................	18	1	2.00	2	0	1	0	0	1	0	0	0	0	0	0	0
.....................................................................GTCTAGAAAGTAGAG............................................................................................................................	15	1	2.00	2	0	0	0	0	0	0	0	0	0	0	0	2
..........................................................................................................CAATGCATTCAAGACC......................................................................................	16	1	2.00	2	0	0	0	0	0	0	0	0	0	0	0	2
......................................................................TCTAGAAAGTAGAGC...........................................................................................................................	15	1	2.00	2	0	0	0	0	0	0	0	0	0	0	0	2
.......................................................................CTAGAAAGTAGAGCT..........................................................................................................................	15	3	1.33	4	0	0	0	0	0	0	0	0	0	0	0	4
................................................................TCAAGGTCTAGAAAGTA...............................................................................................................................	17	1	1.00	1	0	0	0	0	0	0	0	1	0	0	0	0
...................................................................AGGTCTAGAAAGTAGAGCT..........................................................................................................................	19	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	1
..............................................................................................................GCATTCAAGACCTGT-GCG...............................................................................	18	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	1
..........................................................................................................CAATGCATTCAAGACCTGT-GCGG..............................................................................	23	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0
.....................................................................................................TTTGGCAATGCATTCAAGACCTGT-GC................................................................................	26	1	1.00	1	0	0	0	0	0	0	0	1	0	0	0	0
..................................................................AAGGTCTAGAAAGTAGAGC...........................................................................................................................	19	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0
...........................................................................................................................................................................GTTTTGTTTTGCTGG......................	15	18	0.06	1	0	0	0	0	0	0	0	0	0	0	0	1

droSec2

GCTAAGTAA---TAA-TTGAGT-TGGAGC-TCGACTTTTTGTAGTGAAGTATT--------CCCTCAAGGTCTAGAAAGTAGAGCTCAGTAATTTTACAAATTTGGCAATGCATTCAAGACCTGT-GCGGAGTTATTTACACGTCGTCC---ATCAA-----------AGTGTTTTGTTTTGCTGGTTTGA-AA--------------	Size	Hit Count	Total Norm	Total	V113 Male-body	V114 Embryo	V115 Head	M054 Female-body
................................................................TCAAGGTCTAGAAAGTAGAGCT..........................................................................................................................	22	1	66.00	66	36	6	1	23
................................................................TCAAGGTCTAGAAAGTAGAGC...........................................................................................................................	21	1	20.00	20	13	1	3	3
................................................................TCAAGGTCTAGAAAGTAGAG............................................................................................................................	20	1	4.00	4	2	2	0	0
..........................................................................................................CAATGCATTCAAGACCTGT-GCG...............................................................................	22	1	1.00	1	0	0	0	1
.................................................................CAAGGTCTAGAAAGTAGAGC...........................................................................................................................	20	1	1.00	1	0	0	0	1
.................................................................CAAGGTCTAGAAAGTAGAGCT..........................................................................................................................	21	1	1.00	1	0	0	0	1

droEre2

GCTGAGA-A---TTA-TTGCCT-GGAACC-TCGAGAT--TGTTGTGAA--ATT--------CCCTTAAGGTCAGGAAAGTATAGCTCAGTGATTT-GCGAATTTAGCAATACATTCTAGACCTTT-GCGGAGTTTTTCAAAGGTCTTCC---GTGAA-----------AGTGTTCTGCTT----------------------------	Size	Hit Count	Total Norm	Total	V040 Embryo	V060 Head	V107 Male-body	V108 Head	M055 Female-body	M057 Embryo
.................................................................TAAGGTCAGGAAAGTATAGCT..........................................................................................................................	21	1	718.00	718	1	3	589	48	77	0
.................................................................TAAGGTCAGGAAAGTATAGCTC.........................................................................................................................	22	1	430.00	430	1	3	363	38	23	2
.................................................................TAAGGTCAGGAAAGTATAGCTCA........................................................................................................................	23	1	405.00	405	1	0	355	32	17	0
................................................................TTAAGGTCAGGAAAGTATAGCT..........................................................................................................................	22	1	76.00	76	0	0	33	10	32	1
..................................................................AAGGTCAGGAAAGTATAGCTCA........................................................................................................................	22	1	47.00	47	0	0	43	0	4	0
.................................................................TAAGGTCAGGAAAGTATAGC...........................................................................................................................	20	1	32.00	32	0	0	29	2	1	0
..................................................................AAGGTCAGGAAAGTATAGCTC.........................................................................................................................	21	1	27.00	27	0	0	25	0	2	0
................................................................TTAAGGTCAGGAAAGTATAGC...........................................................................................................................	21	1	8.00	8	0	0	7	0	1	0
........................................................................................................AGCAATACATTCTAGACCTTT-G.................................................................................	22	1	8.00	8	0	0	0	2	6	0
..........................................................................................................CAATACATTCTAGACCTTT-GC................................................................................	21	1	7.00	7	0	0	0	0	7	0
..........................................................................................................CAATACATTCTAGACCTTT-GCG...............................................................................	22	1	7.00	7	0	0	3	0	4	0
.................................................................TAAGGTCAGGAAAGTATAG............................................................................................................................	19	1	6.00	6	0	0	4	2	0	0
..........................................................................................................CAATACATTCTAGACCTTT-G.................................................................................	20	1	4.00	4	0	0	0	0	4	0
..................................................................AAGGTCAGGAAAGTATAGCT..........................................................................................................................	20	1	3.00	3	0	0	1	0	2	0
........................................................................................................AGCAATACATTCTAGACCTTT-GC................................................................................	23	1	2.00	2	0	0	0	0	2	0
...........................................................................................................AATACATTCTAGACCTTT-GC................................................................................	20	1	1.00	1	0	0	0	0	1	0
...................................................................AGGTCAGGAAAGTATAGCTCA........................................................................................................................	21	1	1.00	1	0	0	0	0	1	0
.........................................................................................................GCAATACATTCTAGACCTTT-G.................................................................................	21	1	1.00	1	0	0	0	0	1	0
................................................................TTAAGGTCAGGAAAGTATAG............................................................................................................................	20	1	1.00	1	0	0	0	0	1	0
................................................................TTAAGGTCAGGAAAGTATA.............................................................................................................................	19	1	1.00	1	0	0	0	0	1	0
...................................................................AGGTCAGGAAAGTATAGCTC.........................................................................................................................	20	1	1.00	1	0	0	1	0	0	0

droYak3

GCTAAGAAA---C---------------C-AGGACGT--CTTTGTGAATTATT--------CCCTTAAGGTCAGAAATGTATAGCTTAGTGGCTTTACAAATTTGGCAATACATTTAAGACCTTA-GCGGAGTTTCTCACATGCCTTCC---ATAAA-----------AGTGTCTAGTCTAGACAATTTAA-AA--------------	Size	Hit Count	Total Norm	Total	V046 Embryo	V052 Head	V120 Male-body	V058 Head	M026 Head	M043 Female-body	M056 Embryo
................................................................TTAAGGTCAGAAATGTATAGCT..........................................................................................................................	22	1	626.00	626	1	0	311	5	214	78	17
.................................................................TAAGGTCAGAAATGTATAGCT..........................................................................................................................	21	1	95.00	95	1	0	34	0	43	16	1
..........................................................................................................CAATACATTTAAGACCTTA-GCG...............................................................................	22	1	74.00	74	0	0	25	1	0	43	5
.................................................................TAAGGTCAGAAATGTATAGCTT.........................................................................................................................	22	1	28.00	28	0	0	21	1	4	1	1
..........................................................................................................CAATACATTTAAGACCTTA-GC................................................................................	21	1	21.00	21	0	0	2	0	0	12	7
................................................................TTAAGGTCAGAAATGTATAGC...........................................................................................................................	21	1	19.00	19	0	0	13	0	5	1	0
................................................................TTAAGGTCAGAAATGTATAG............................................................................................................................	20	1	5.00	5	0	0	5	0	0	0	0
................................................................TTAAGGTCAGAAATGTATAGCTT.........................................................................................................................	23	1	4.00	4	0	0	3	0	0	1	0
...........................................................................................................AATACATTTAAGACCTTA-GC................................................................................	20	1	4.00	4	0	0	0	0	0	4	0
..........................................................................................................CAATACATTTAAGACCTTA-G.................................................................................	20	1	3.00	3	0	0	0	0	0	3	0
...........................................................................................................AATACATTTAAGACCTTA-GCG...............................................................................	21	1	3.00	3	0	0	0	0	0	3	0
..............................................................................................................ACATTTAAGACCTTA-GCG...............................................................................	18	1	1.00	1	0	0	0	0	0	1	0

droEug1

GCAAAGTAAA--TCATTTAACT-TACACT-TTCATTTCAAATTGTGA-GTATT--------TTCTTAAGGTCTGAACAGTATAGCTCAGTAACTTAAGAAATTCTGCAATACATTCAAGACTT-------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droTak1

GGTGCGTAA---GAA-TTAAAT----------AATTT--TCTTGTGAACTTTT--------TCCTTAAGGTCTGGGAAGTATAGCTAAGTGATTTTAAATATTCTGCAATGCATTCAAGACCTTTTGCAAAG-GGTTCACACGTGTTTCTTTATTGAATTGTTTATGAAGTGT---------CTGTTTTGA-AAGAAACTTAAAATTC	Size	Hit Count	Total Norm	Total
No Reads

droEle1

AAGAAGTTTT--TAATTTGTGT-T------AAAACAT--TTTTGTGAAATATT--------TTCTTAAGGTCTTGAAGATATACCTTAGTAATTTTAAAAAGTCGGTAATATATTCAAGATCTTCTGTAAAGTGTTTTTTTCATGTTTCTT-GTTAC-----------TCCCTT----------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droRho1

GTGAACAAAT--TAA-TTTCTT-TGGGCT-TTAACTT--TTTTGTGAAATATT--------TTCTTAAGGTCTGGAAAGTATATCAATGTATATTAAAAAATTCTGCTATATTTTAAAGACCTTTTGCAAAGTGCTTTACTCGTGATTC-----------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droFic1

GGGAATTCCTCCGAA-GTGACT-AAG-CCGCAGAAGT--GTCTGTGATATATT--------TTCTTAAGGTCTGGAAAGTATAGCAACGGCATTTTA-AACTCCTGCGATACATTCAAGACCTTT-TGTAAATTTCCTACTCGTGGTTC---A-------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

dsi_6

X:16526668-16526731 -

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-26.9	-26.4	-26.2