dsi_180

TTCAAGTTTTGGAATTCTGAGAGGTTTCAATTTTTTGCAAATTTTGATGATGGTACCCCTTACAAAAAATTCGAAAATTTGGCCAAAAATTAATTTTACAAAATCAGTTTAAAAGTGAAAGGGGATGTTAGTATTGTATTGTAAGGAGTACAAAATGGTACTCCTTTTTGCTCTCT ************************************........((((((.((((((((.((..(((((.((...((((.................)))).))))))).....)).))))))....)).))))))...**********************************	Read size	# Mismatch	Hit Count	Total Norm	Total	O001 Testis	SRR553486 Ovary	SRR553487 Ovary	SRR553488 Ovary	SRR553485 Ovary	O002 Head	SRR618934 Ovary
.......................................................................................................TCAGTTTAAAAGTGAAAGGGGA...................................................	22	0	2	17.50	35	0	19	6	7	3	0	0
........................................................................................................CAGTTTAAAAGTGAAAGGGGA...................................................	21	0	2	10.00	20	20	0	0	0	0	0	0
........................................................................................................CAGTTTAAAAGTGAAAGGGGATG.................................................	23	0	2	6.00	12	12	0	0	0	0	0	0
...........................................................................................................................GATGTTAGTATTGTATTGTAAGGAG............................	25	0	1	2.00	2	0	1	1	0	0	0	0
...................................................................................................AAAATCAGTTTAAAAGTGAAAGGGGA...................................................	26	0	2	1.50	3	0	3	0	0	0	0	0
...........................................................................................................................GATGTTAGTATTGTATTGTAAGGAGT...........................	26	0	1	1.00	1	0	1	0	0	0	0	0
....................................................................................................AAATCAGTTTAAAAGTGAAAGGGGA...................................................	25	0	2	1.00	2	0	1	0	0	1	0	0
........................................................................................................CAGTTTAAAAGTGAAAGGGGAT..................................................	22	0	2	1.00	2	2	0	0	0	0	0	0
........................................................................................................CAGTTTAAAAGTGAAAGGGGATGTTA..............................................	26	0	2	1.00	2	2	0	0	0	0	0	0
...............................................................................................TTACAAAATCAGTTTAAAAGTGAAAGGGGA...................................................	30	0	1	1.00	1	0	1	0	0	0	0	0
.....................................................................................................AATCAGTTTAAAAGTGAAAGGGGA...................................................	24	0	2	1.00	2	0	0	0	0	0	2	0
.......................................................................................................TCAGTTTAAAAGTGAAAGGGTA...................................................	22	1	13	1.00	13	0	3	3	7	0	0	0
..................................................................................................CAAAATCAGTTTAAAAGTGAAAGGGGA...................................................	27	0	2	1.00	2	0	2	0	0	0	0	0
................................................................................................TACAAAATCAGTTTAAAAGTGAAAGGGGA...................................................	29	0	1	1.00	1	0	1	0	0	0	0	0
.......................................................................................................TCAGTTTAAAAGTGAAAGGGGATA.................................................	24	1	5	0.60	3	0	2	0	1	0	0	0
............................................................................................................TTAAAAGTGAAAGGGGA...................................................	17	0	2	0.50	1	0	1	0	0	0	0	0
..............................................................................................................AAAAGTGAAAGGGGATGTTAGTATT.........................................	25	0	2	0.50	1	0	0	0	0	0	0	1
......................................................................................................ATCAGTTTAAAAGTGAAAGGGGA...................................................	23	0	2	0.50	1	0	1	0	0	0	0	0
TTCAAGTTTTGGAATTCTGAGAGGTT......................................................................................................................................................	26	0	18	0.39	7	0	0	0	0	7	0	0
..............................................................................................TTTACAAAATCAGTTTAAAAGTGAA.........................................................	25	0	20	0.35	7	0	0	7	0	0	0	0
.....................................................TACCCCTTACAAAAAATTCGAAAATT.................................................................................................	26	0	20	0.30	6	0	0	6	0	0	0	0
..........GGAATTCTGAGTGGT.......................................................................................................................................................	15	1	20	0.25	5	0	5	0	0	0	0	0
..CAAGTTTTGGAATTCTGAGAGGTT......................................................................................................................................................	24	0	18	0.22	4	0	0	3	0	1	0	0
.......................................................................................................TCAGTTTAAAAGTGAAAGTGGA...................................................	22	1	5	0.20	1	0	0	1	0	0	0	0
.......................................................................................................TCAGTTTAAAAGCGAAAGGGGA...................................................	22	1	5	0.20	1	0	0	0	1	0	0	0
.......................................................................................................TCAGTTTAAAAGTGAAAGGGGAA..................................................	23	1	19	0.16	3	0	1	1	1	0	0	0
.......................................................................................................TCAGTTTAAAAGTGAAACGGTA...................................................	22	2	19	0.16	3	0	0	0	3	0	0	0
......................................................................................................................................TGTATTGTAAGGAGTACA........................	18	0	7	0.14	1	0	0	0	0	0	0	1
.......................................................................................................TCAGTTTAAAAGTGAAAGGGGACA.................................................	24	2	7	0.14	1	0	1	0	0	0	0	0
......................................................................................................ATCAGTTTAAAGGTGAAAGGGGA...................................................	23	1	8	0.13	1	0	1	0	0	0	0	0
.......................................................................................................................................GTATTGTAAGCAGTAGAAA......................	19	2	9	0.11	1	0	0	0	1	0	0	0
TTCAAGTTTTGGAATTCTGAGAGGT.......................................................................................................................................................	25	0	18	0.11	2	0	0	0	0	2	0	0
.....................................................................................................................AATGGGGATGGTAGAATTGT.......................................	20	3	9	0.11	1	0	0	0	0	0	0	0
.TCAAGTTTTGGAATTCTGAGAGGTT......................................................................................................................................................	25	0	18	0.11	2	0	0	1	0	1	0	0
.....................................................................................................................................TAGTATTGTAAGGAG............................	15	1	10	0.10	1	0	0	0	0	0	0	1
...............................................................................................TTACAAAATCAGTTTAAAAGTGAAAGG......................................................	27	0	20	0.10	2	0	2	0	0	0	0	0
................................................................................................TACAAAATCAGTTTAAAAGTAAAAGGGGA...................................................	29	1	20	0.10	2	0	2	0	0	0	0	0
.................................TTTGCAAATTTTGATGATGGTACCC......................................................................................................................	25	0	20	0.10	2	0	0	0	0	2	0	0
.......................................................................................................TCAGTTTAAAAGTGAAAGGAGA...................................................	22	1	20	0.10	2	0	0	0	2	0	0	0
.......................................................................................................TCAGTTTAAAAGTGAAAGGGCA...................................................	22	1	20	0.10	2	0	0	0	2	0	0	0
......................................................................................................ATCAGTTTAAAAGTGAAAGGGTA...................................................	23	1	13	0.08	1	0	0	0	1	0	0	0
..................................................................................................TAAAATCAGTTTAAAAGTGAAAGGGGA...................................................	27	1	13	0.08	1	0	1	0	0	0	0	0
.......................................................................................................TCAGTTTCAAAGTGAAAGGGTA...................................................	22	2	14	0.07	1	0	0	0	1	0	0	0
..................................................................................................CAAAATCAGTTTTAAAGTGAAAGGGTA...................................................	27	2	15	0.07	1	0	0	1	0	0	0	0
.......................................................................................................TCAGTTTAAGAGTGAAAGGGTA...................................................	22	2	16	0.06	1	0	0	0	1	0	0	0
.........................................................................................................................................AGTGTAAGGAGTACAAAATGGTA................	23	1	16	0.06	1	0	0	0	0	0	0	1
TTCAAGTTTTGGAATTCTGAGAGGTTTCAA..................................................................................................................................................	30	0	18	0.06	1	0	1	0	0	0	0	0
..CAAGTTTTGGAATTCTGAGAGGTTTC....................................................................................................................................................	26	0	18	0.06	1	0	0	1	0	0	0	0
.TCAAGTTTTGGAATTCTGAGAGGTTTCAA..................................................................................................................................................	29	0	18	0.06	1	0	0	0	0	1	0	0
.TCAAGTTTTGGAATTCTGAGAGGT.......................................................................................................................................................	24	0	18	0.06	1	0	0	0	0	1	0	0
..CAAGTTTTGGAATTCTGAGAGGTTTCAA..................................................................................................................................................	28	0	18	0.06	1	0	0	1	0	0	0	0
...AAGTTTTGGAATTCTGAGAGGTT......................................................................................................................................................	23	0	18	0.06	1	0	0	1	0	0	0	0
TTCAAGTTTTGGAATTCTGAGAGG........................................................................................................................................................	24	0	18	0.06	1	0	0	0	0	1	0	0
................................................................................................TACAAAATCAGTTTAAAAGTGAAACGGTA...................................................	29	2	18	0.06	1	0	0	0	0	1	0	0
..................................................................................................CAAAATCAGTTTAAAAGTGAAACGGTA...................................................	27	2	19	0.05	1	0	0	1	0	0	0	0
...AAGTTTTGGAATTCTGAGA..........................................................................................................................................................	19	0	19	0.05	1	0	0	0	0	0	0	1
.................................................................................................ACAAAATCAGTTTAAAAGT............................................................	19	0	20	0.05	1	0	0	0	0	0	0	1
.....................................................TACCCCTTACAAAAAAT..........................................................................................................	17	0	20	0.05	1	0	1	0	0	0	0	0
TTCAAGTTTTGGAATTCTGAGAGGTGCA....................................................................................................................................................	28	3	20	0.05	1	0	1	0	0	0	0	0
........................................................................................................TAGTTTAAAAGTGAAAGGGGA...................................................	21	1	20	0.05	1	0	1	0	0	0	0	0
.....................................................TACCCCTTACAAAAAATTCGAAAA...................................................................................................	24	0	20	0.05	1	0	0	0	0	1	0	0
...............................................................................................TTACAAAATCAGTTTAAAAG.............................................................	20	0	20	0.05	1	0	0	0	0	0	0	1
........................................................................................................CAGTTTAAAAGTGAAAGGG.....................................................	19	0	20	0.05	1	0	1	0	0	0	0	0
...........................................................................................................TTTAAAAGTGAAAGGGTATTCT...............................................	22	3	20	0.05	1	0	0	1	0	0	0	0
.......................................................................................................TCAGTTTAAAAGTGAAAGGGTAAA.................................................	24	3	20	0.05	1	0	1	0	0	0	0	0
............................................................................................................TTAAAAGTGAAAGGGTATT.................................................	19	2	20	0.05	1	0	0	0	1	0	0	0
.........................TTCAATTTTTTGCAAATTTTGATGA..............................................................................................................................	25	0	20	0.05	1	0	0	0	0	1	0	0
.................................................ATGCTACCCCTTACAAAAAATTCGAA.....................................................................................................	26	1	20	0.05	1	0	0	0	1	0	0	0
.......................................................................................................TCAGTTCAAAAGTGAAAGTCGA...................................................	22	3	20	0.05	1	0	0	0	1	0	0	0
..................................................................................................CAAAATCAGTTTAAAAGTAAAAGGGGA...................................................	27	1	20	0.05	1	0	1	0	0	0	0	0
.......................................................................................................TCAGTTTAAAAGTAAAAGGGGA...................................................	22	1	20	0.05	1	0	0	0	1	0	0	0
.......................................................................................................TCAGTTTAAAAGCGAAAGGGTAA..................................................	23	3	20	0.05	1	0	1	0	0	0	0	0
.............................ATTTTTTGCAAATTTTGATGATGG...........................................................................................................................	24	0	20	0.05	1	0	1	0	0	0	0	0
.......................................................................................................TCAGTTTAAAAGTGAAAGGGTATT.................................................	24	2	20	0.05	1	0	1	0	0	0	0	0
..........CGAATTCTGAGAGGT.......................................................................................................................................................	15	1	20	0.05	1	0	0	0	0	1	0	0
.......................................................................................................TCAGTTTAAAAGTGAAAGGGTAA..................................................	23	2	20	0.05	1	0	0	0	1	0	0	0

TTCAAGTTTTGGAATTCTGAGAGGTTTCAATTTTTTGCAAATTTTGATGATGGTACCCCTTACAAAAAATTCGAAAATTTGGCCAAAAATTAATTTTACAAAATCAGTTTAAAAGTGAAAGGGGATGTTAGTATTGTATTGTAAGGAGTACAAAATGGTACTCCTTTTTGCTCTCT
**************************************........((((((.((((((((.((..(((((.((...((((.................)))).))))))).....)).))))))....)).))))))...************************************

Read size

# Mismatch

Hit Count

Total Norm

Total

O001
Testis

SRR553486
Ovary

SRR553487
Ovary

SRR553488
Ovary

SRR553485
Ovary

O002
Head

SRR618934
Ovary

.......................................................................................................TCAGTTTAAAAGTGAAAGGGGA...................................................

17.50

........................................................................................................CAGTTTAAAAGTGAAAGGGGA...................................................

10.00

........................................................................................................CAGTTTAAAAGTGAAAGGGGATG.................................................

6.00

...........................................................................................................................GATGTTAGTATTGTATTGTAAGGAG............................

2.00

...................................................................................................AAAATCAGTTTAAAAGTGAAAGGGGA...................................................

1.50

...........................................................................................................................GATGTTAGTATTGTATTGTAAGGAGT...........................

1.00

....................................................................................................AAATCAGTTTAAAAGTGAAAGGGGA...................................................

1.00

........................................................................................................CAGTTTAAAAGTGAAAGGGGAT..................................................

1.00

........................................................................................................CAGTTTAAAAGTGAAAGGGGATGTTA..............................................

1.00

...............................................................................................TTACAAAATCAGTTTAAAAGTGAAAGGGGA...................................................

1.00

.....................................................................................................AATCAGTTTAAAAGTGAAAGGGGA...................................................

1.00

.......................................................................................................TCAGTTTAAAAGTGAAAGGGTA...................................................

1.00

..................................................................................................CAAAATCAGTTTAAAAGTGAAAGGGGA...................................................

1.00

................................................................................................TACAAAATCAGTTTAAAAGTGAAAGGGGA...................................................

1.00

.......................................................................................................TCAGTTTAAAAGTGAAAGGGGATA.................................................

0.60

............................................................................................................TTAAAAGTGAAAGGGGA...................................................

0.50

..............................................................................................................AAAAGTGAAAGGGGATGTTAGTATT.........................................

0.50

......................................................................................................ATCAGTTTAAAAGTGAAAGGGGA...................................................

0.50

TTCAAGTTTTGGAATTCTGAGAGGTT......................................................................................................................................................

0.39

..............................................................................................TTTACAAAATCAGTTTAAAAGTGAA.........................................................

0.35

.....................................................TACCCCTTACAAAAAATTCGAAAATT.................................................................................................

0.30

..........GGAATTCTGAGTGGT.......................................................................................................................................................

0.25

..CAAGTTTTGGAATTCTGAGAGGTT......................................................................................................................................................

0.22

.......................................................................................................TCAGTTTAAAAGTGAAAGTGGA...................................................

0.20

.......................................................................................................TCAGTTTAAAAGCGAAAGGGGA...................................................

0.20

.......................................................................................................TCAGTTTAAAAGTGAAAGGGGAA..................................................

0.16

.......................................................................................................TCAGTTTAAAAGTGAAACGGTA...................................................

0.16

......................................................................................................................................TGTATTGTAAGGAGTACA........................

0.14

.......................................................................................................TCAGTTTAAAAGTGAAAGGGGACA.................................................

0.14

......................................................................................................ATCAGTTTAAAGGTGAAAGGGGA...................................................

0.13

.......................................................................................................................................GTATTGTAAGCAGTAGAAA......................

0.11

TTCAAGTTTTGGAATTCTGAGAGGT.......................................................................................................................................................

0.11

.....................................................................................................................AATGGGGATGGTAGAATTGT.......................................

0.11

.TCAAGTTTTGGAATTCTGAGAGGTT......................................................................................................................................................

0.11

.....................................................................................................................................TAGTATTGTAAGGAG............................

0.10

...............................................................................................TTACAAAATCAGTTTAAAAGTGAAAGG......................................................

0.10

................................................................................................TACAAAATCAGTTTAAAAGTAAAAGGGGA...................................................

0.10

.................................TTTGCAAATTTTGATGATGGTACCC......................................................................................................................

0.10

.......................................................................................................TCAGTTTAAAAGTGAAAGGAGA...................................................

0.10

.......................................................................................................TCAGTTTAAAAGTGAAAGGGCA...................................................

0.10

......................................................................................................ATCAGTTTAAAAGTGAAAGGGTA...................................................

0.08

..................................................................................................TAAAATCAGTTTAAAAGTGAAAGGGGA...................................................

0.08

.......................................................................................................TCAGTTTCAAAGTGAAAGGGTA...................................................

0.07

..................................................................................................CAAAATCAGTTTTAAAGTGAAAGGGTA...................................................

0.07

.......................................................................................................TCAGTTTAAGAGTGAAAGGGTA...................................................

0.06

.........................................................................................................................................AGTGTAAGGAGTACAAAATGGTA................

0.06

TTCAAGTTTTGGAATTCTGAGAGGTTTCAA..................................................................................................................................................

0.06

..CAAGTTTTGGAATTCTGAGAGGTTTC....................................................................................................................................................

0.06

.TCAAGTTTTGGAATTCTGAGAGGTTTCAA..................................................................................................................................................

0.06

.TCAAGTTTTGGAATTCTGAGAGGT.......................................................................................................................................................

0.06

..CAAGTTTTGGAATTCTGAGAGGTTTCAA..................................................................................................................................................

0.06

...AAGTTTTGGAATTCTGAGAGGTT......................................................................................................................................................

0.06

TTCAAGTTTTGGAATTCTGAGAGG........................................................................................................................................................

0.06

................................................................................................TACAAAATCAGTTTAAAAGTGAAACGGTA...................................................

0.06

..................................................................................................CAAAATCAGTTTAAAAGTGAAACGGTA...................................................

0.05

...AAGTTTTGGAATTCTGAGA..........................................................................................................................................................

0.05

.................................................................................................ACAAAATCAGTTTAAAAGT............................................................

0.05

.....................................................TACCCCTTACAAAAAAT..........................................................................................................

0.05

TTCAAGTTTTGGAATTCTGAGAGGTGCA....................................................................................................................................................

0.05

........................................................................................................TAGTTTAAAAGTGAAAGGGGA...................................................

0.05

.....................................................TACCCCTTACAAAAAATTCGAAAA...................................................................................................

0.05

...............................................................................................TTACAAAATCAGTTTAAAAG.............................................................

0.05

........................................................................................................CAGTTTAAAAGTGAAAGGG.....................................................

0.05

...........................................................................................................TTTAAAAGTGAAAGGGTATTCT...............................................

0.05

.......................................................................................................TCAGTTTAAAAGTGAAAGGGTAAA.................................................

0.05

............................................................................................................TTAAAAGTGAAAGGGTATT.................................................

0.05

.........................TTCAATTTTTTGCAAATTTTGATGA..............................................................................................................................

0.05

.................................................ATGCTACCCCTTACAAAAAATTCGAA.....................................................................................................

0.05

.......................................................................................................TCAGTTCAAAAGTGAAAGTCGA...................................................

0.05

..................................................................................................CAAAATCAGTTTAAAAGTAAAAGGGGA...................................................

0.05

.......................................................................................................TCAGTTTAAAAGTAAAAGGGGA...................................................

0.05

.......................................................................................................TCAGTTTAAAAGCGAAAGGGTAA..................................................

0.05

.............................ATTTTTTGCAAATTTTGATGATGG...........................................................................................................................

0.05

.......................................................................................................TCAGTTTAAAAGTGAAAGGGTATT.................................................

0.05

..........CGAATTCTGAGAGGT.......................................................................................................................................................

0.05

.......................................................................................................TCAGTTTAAAAGTGAAAGGGTAA..................................................

0.05

AGAGAGCAAAAAGGAGTACCATTTTGTACTCCTTACAATACAATACTAACATCCCCTTTCACTTTTAAACTGATTTTGTAAAATTAATTTTTGGCCAAATTTTCGAATTTTTTGTAAGGGGTACCATCATCAAAATTTGCAAAAAATTGAAACCTCTCAGAATTCCAAAACTTGAA **********************************...((((((.((....((((((.((.....(((((((.((((.................))))...)).)))))..)).)))))))).))))))........************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	SRR553485 Ovary	SRR553488 Ovary	SRR553487 Ovary	O001 Testis	SRR553486 Ovary	SRR618934 Ovary	M053 Female-body
.................................................................................................................................................ATTGAAACCTCTCAGAATTCCAAAAC.....	26	0	18	1.28	23	3	0	2	18	0	0	0
.....................................ATACAATACTAACATCCCCTTTTAC..................................................................................................................	25	1	1	1.00	1	0	0	0	0	1	0	0
..................................................................................................................................................TTGAAACCTCTCAGAATTCCAAAA......	24	0	18	0.67	12	1	6	3	0	2	0	0
...................................................................................................................................................TGAAACCTCTCAGAATTCCAAAA......	23	0	18	0.50	9	1	3	0	0	5	0	0
..................................................................................................................................................TTGAAACCTCTCAGAATTCCAAAAA.....	25	1	18	0.33	6	2	2	2	0	0	0	0
.........................................................................................TTTGGCCAAATTTTCGAATTTTTTGTA............................................................	27	0	20	0.30	6	6	0	0	0	0	0	0
..................................................................................................................................................TTGAAACCTCTCAGAATTCCAAA.......	23	0	18	0.28	5	2	3	0	0	0	0	0
.................................................................................................................................................ATTGAAACCTCTCAGAATTCCAAAA......	25	0	18	0.28	5	2	0	3	0	0	0	0
.....................................................................................................TTCGAATTTTTTGTAAGGGGTACCATC................................................	27	0	20	0.25	5	0	0	5	0	0	0	0
...............................................................................................................TTGTAAGGGGTACCATCAT..............................................	19	0	20	0.20	4	0	0	0	0	0	4	0
.....................................................................................................TTCGGATTTTTTGTAAGGGGTACCATC................................................	27	1	20	0.20	4	0	0	4	0	0	0	0
....................................................................................TAATTTTTGGCCAAATTTTCGAAT....................................................................	24	0	20	0.20	4	0	0	2	0	0	0	2
.................................................................................................................................................ATTGAAACCTCTCAGAATTCCAAA.......	24	0	18	0.17	3	2	0	1	0	0	0	0
............................................................................................GGCCAAATTTTCGAATTTTTTGTAAG..........................................................	26	0	20	0.15	3	0	2	1	0	0	0	0
..............................................................................TAAAATTAATTTTTGGCCAAATTTTCGAA.....................................................................	29	0	20	0.15	3	3	0	0	0	0	0	0
.....................................................................................................................GGGGTACCATCATCAAAATTTGCAAAA................................	27	0	20	0.15	3	0	3	0	0	0	0	0
.......................................................................................................................................TTTGCAAAAAATTGAAACCTCTCAGAA..............	27	0	15	0.13	2	1	1	0	0	0	0	0
...................................................................................................................................................TGAAACCTCTCAGAATTCCAAA.......	22	0	18	0.11	2	0	2	0	0	0	0	0
...................................................................................................................................................TGAAACCTCTCAGAATTCCAAAAA.....	24	1	18	0.11	2	1	0	0	0	1	0	0
....................................................................ACTGATTTTGTAAAATTAATTTTTGGC.................................................................................	27	0	20	0.10	2	2	0	0	0	0	0	0
..............................................................................................................TTTGTAAGGGGTACCATCA...............................................	19	0	20	0.10	2	0	0	0	0	0	2	0
.....................................................................................AATTTTTGGCCAAATTTTCGAATTTT.................................................................	26	0	20	0.10	2	0	0	0	0	0	0	2
............................................................................................GGCCAAATTTTCGAATTTTTTGTAA...........................................................	25	0	20	0.10	2	0	0	0	0	2	0	0
...................................................................................................TTTTCGAATTTTTTGTAAGGGGTACCATC................................................	29	0	20	0.10	2	2	0	0	0	0	0	0
...........................................................................................TGGCCAAATTGTCGAATTTTTTGTA............................................................	25	1	20	0.10	2	0	0	0	0	2	0	0
.................................................................................................................................................ATTGAAACCTCTCAGAATTCCAAAACT....	27	0	18	0.06	1	1	0	0	0	0	0	0
.................................................................................................................................................ATTGAAACCTATCAGAATTCCAAAACT....	27	1	18	0.06	1	1	0	0	0	0	0	0
...................................................................................................................................................TGAAACCTCTCAGAATTCCAA........	21	0	18	0.06	1	0	1	0	0	0	0	0
...................................................................................................................................................TGAAACCTCTCAGAATTCCAAAATA....	25	2	18	0.06	1	0	0	1	0	0	0	0
...................................................................................................................................................TGAAACCTCTCAGAATTCCAAAACT....	25	0	18	0.06	1	0	0	1	0	0	0	0
..................................................................................................................................................TTGAAACCTCTCAGAATTCCAAAACT....	26	0	18	0.06	1	0	0	0	0	1	0	0
...................................................................................................................................................TGAAACCTCTCAGAATTCCAAAAAAA...	26	3	19	0.05	1	0	1	0	0	0	0	0
.......................................................................................TTTTTGGCCAAATTTTCGAA.....................................................................	20	0	20	0.05	1	0	0	0	0	0	1	0
......................................................................TGATTTTGTAAAATTAGTTTTTGGCCAAA.............................................................................	29	1	20	0.05	1	0	0	1	0	0	0	0
..........................................................................................TTGGCCAAATTTTCGAATTTTTTGTAAGGGG.......................................................	31	0	20	0.05	1	0	0	0	0	1	0	0
.......................................................................................................................................................ACCTCGCAGAACTCAAAAAC.....	20	3	20	0.05	1	0	0	0	0	0	0	0
....................................................................................................................AGGGGTACCATCATCAAAATTTG.....................................	23	0	20	0.05	1	0	0	0	0	0	1	0
.....................................................................................................TTCGGATTTTTTGTAAGGGGTACCATCA...............................................	28	1	20	0.05	1	0	0	1	0	0	0	0
.......................................................................................TTGTTGGCCAAATTTTCGAATTTTTTGTA............................................................	29	1	20	0.05	1	1	0	0	0	0	0	0
........................................................TTTCACTTTTAAACTGATTTTGTAA...............................................................................................	25	0	20	0.05	1	0	0	0	0	0	0	1
...........................................................................................................TTTTTTGTAAGGGGTACCATCATCAAAA.........................................	28	0	20	0.05	1	0	1	0	0	0	0	0
...........................................................................................TGGCCAAATTTTCGAATTTTTTGTAAGGGG.......................................................	30	0	20	0.05	1	0	0	0	0	1	0	0
...............................................................TTTAAACTGATTTTGTAAAATTAATT.......................................................................................	26	0	20	0.05	1	0	1	0	0	0	0	0
.....................................................................................................................GGGGTACCATCATCAAAATTTGCA...................................	24	0	20	0.05	1	0	1	0	0	0	0	0
...................................................................................................TTTTCGAATTTTTTGTAAGGGGTACCA..................................................	27	0	20	0.05	1	0	0	1	0	0	0	0
...................................................................................................TTTTCGAATTTTTTGT.............................................................	16	0	20	0.05	1	1	0	0	0	0	0	0

AGAGAGCAAAAAGGAGTACCATTTTGTACTCCTTACAATACAATACTAACATCCCCTTTCACTTTTAAACTGATTTTGTAAAATTAATTTTTGGCCAAATTTTCGAATTTTTTGTAAGGGGTACCATCATCAAAATTTGCAAAAAATTGAAACCTCTCAGAATTCCAAAACTTGAA
************************************...((((((.((....((((((.((.....(((((((.((((.................))))...)).)))))..)).)))))))).))))))........**************************************

Read size

# Mismatch

Hit Count

Total Norm

Total

SRR553485
Ovary

SRR553488
Ovary

SRR553487
Ovary

O001
Testis

SRR553486
Ovary

SRR618934
Ovary

M053
Female-body

.................................................................................................................................................ATTGAAACCTCTCAGAATTCCAAAAC.....

1.28

.....................................ATACAATACTAACATCCCCTTTTAC..................................................................................................................

1.00

..................................................................................................................................................TTGAAACCTCTCAGAATTCCAAAA......

0.67

...................................................................................................................................................TGAAACCTCTCAGAATTCCAAAA......

0.50

..................................................................................................................................................TTGAAACCTCTCAGAATTCCAAAAA.....

0.33

.........................................................................................TTTGGCCAAATTTTCGAATTTTTTGTA............................................................

0.30

..................................................................................................................................................TTGAAACCTCTCAGAATTCCAAA.......

0.28

.................................................................................................................................................ATTGAAACCTCTCAGAATTCCAAAA......

0.28

.....................................................................................................TTCGAATTTTTTGTAAGGGGTACCATC................................................

0.25

...............................................................................................................TTGTAAGGGGTACCATCAT..............................................

0.20

.....................................................................................................TTCGGATTTTTTGTAAGGGGTACCATC................................................

0.20

....................................................................................TAATTTTTGGCCAAATTTTCGAAT....................................................................

0.20

.................................................................................................................................................ATTGAAACCTCTCAGAATTCCAAA.......

0.17

............................................................................................GGCCAAATTTTCGAATTTTTTGTAAG..........................................................

0.15

..............................................................................TAAAATTAATTTTTGGCCAAATTTTCGAA.....................................................................

0.15

.....................................................................................................................GGGGTACCATCATCAAAATTTGCAAAA................................

0.15

.......................................................................................................................................TTTGCAAAAAATTGAAACCTCTCAGAA..............

0.13

...................................................................................................................................................TGAAACCTCTCAGAATTCCAAA.......

0.11

...................................................................................................................................................TGAAACCTCTCAGAATTCCAAAAA.....

0.11

....................................................................ACTGATTTTGTAAAATTAATTTTTGGC.................................................................................

0.10

..............................................................................................................TTTGTAAGGGGTACCATCA...............................................

0.10

.....................................................................................AATTTTTGGCCAAATTTTCGAATTTT.................................................................

0.10

............................................................................................GGCCAAATTTTCGAATTTTTTGTAA...........................................................

0.10

...................................................................................................TTTTCGAATTTTTTGTAAGGGGTACCATC................................................

0.10

...........................................................................................TGGCCAAATTGTCGAATTTTTTGTA............................................................

0.10

.................................................................................................................................................ATTGAAACCTCTCAGAATTCCAAAACT....

0.06

.................................................................................................................................................ATTGAAACCTATCAGAATTCCAAAACT....

0.06

...................................................................................................................................................TGAAACCTCTCAGAATTCCAA........

0.06

...................................................................................................................................................TGAAACCTCTCAGAATTCCAAAATA....

0.06

...................................................................................................................................................TGAAACCTCTCAGAATTCCAAAACT....

0.06

..................................................................................................................................................TTGAAACCTCTCAGAATTCCAAAACT....

0.06

...................................................................................................................................................TGAAACCTCTCAGAATTCCAAAAAAA...

0.05

.......................................................................................TTTTTGGCCAAATTTTCGAA.....................................................................

0.05

......................................................................TGATTTTGTAAAATTAGTTTTTGGCCAAA.............................................................................

0.05

..........................................................................................TTGGCCAAATTTTCGAATTTTTTGTAAGGGG.......................................................

0.05

.......................................................................................................................................................ACCTCGCAGAACTCAAAAAC.....

0.05

....................................................................................................................AGGGGTACCATCATCAAAATTTG.....................................

0.05

.....................................................................................................TTCGGATTTTTTGTAAGGGGTACCATCA...............................................

0.05

.......................................................................................TTGTTGGCCAAATTTTCGAATTTTTTGTA............................................................

0.05

........................................................TTTCACTTTTAAACTGATTTTGTAA...............................................................................................

0.05

...........................................................................................................TTTTTTGTAAGGGGTACCATCATCAAAA.........................................

0.05

...........................................................................................TGGCCAAATTTTCGAATTTTTTGTAAGGGG.......................................................

0.05

...............................................................TTTAAACTGATTTTGTAAAATTAATT.......................................................................................

0.05

.....................................................................................................................GGGGTACCATCATCAAAATTTGCA...................................

0.05

...................................................................................................TTTTCGAATTTTTTGTAAGGGGTACCA..................................................

0.05

...................................................................................................TTTTCGAATTTTTTGT.............................................................

0.05

Species	Coordinate	ID	Alignment
droSim3	chrU_M_2695:832-1007 -	dsi_180	TTCAAGT--TTTGGAATTCTGAG----------A-GGTT-TCAATTTTTTGCAA-ATTTTGATGATGG-----TACCCCTTACAAAAAATTCGA-AAATTTGGCCA-AAAATTAATTTTA-CAAAATCAGTTTAAAAGTGAAA------------GGGGATGTTAGTA--TTGTATTGTAAGGAGTA---CAAAATGGTACTCCTTTTTGCTCTCT
droSec2	scaffold_4622:770-944 +		TTCAAGT--TTTGGAATTCTAGG----------A-GGTT-TCAATTTTTTGCAA-ATTTTGATGATGG-----TACCCCTTACAAAAAATTCGA-AAATTTGGCCA-AAAATTAATTTTA-CAAAATCAGTTTAAAAGTGAAA------------GGGGTTGTTAGTA--TTG-GTTGTAAGGAGTA---CAAAATGGTACTCCTTTTTGCTCTCT
dm3	chrX:8721644-8721818 +		TTCAAGT--TTTGGAGTTCTAGG----------A-TTTT-TCAATTTTTTGCAA-ATTTTGATGATGT-----TACCCCTTACAAAAAATGCGA-AAATTTGGCCA-AAAATTAATTTTA-CAAAATCAGTTTAAAAGTGAAA------------GGGGTTGTTAGTA--TTG-GTTGTAAGGAGTA---TAAAATGGTACTACTTTTTGCTCTGT
droEre2	scaffold_4690:3253882-3254056 +		TTCAAAT--TTGGAAATTTTAAT----------T-TTTT-TCAATTTTTTGCAA-ATTTTGATGATGG-----TACCCCTTACAAAAAATGCGA-AAATTGACCAA-AAAATTGATTTTC-CCAACTCGGTCAAAAAGTGATA------------GGGATGTTTAGTA--TTG-GTAATTAGGAGCA---CAAAACGGTAATTACTTTATCTGTGC
droYak3	X:7880819-7880993 +		TTTTCAA--AAAAAAATTCTATG----------T-CGTT-CCAATTTTTTGCAA-ATTTTGATGATGG-----TACCCCTTACAAAAAATGCGA-AAAAATGGCCA-AAAATTATTTTAA-CACAGTCGGTTAAAAAGTGATT------------TGGTTGGTTAGTA--TTG-GTAATTAGGAGTA---AAAAACTGTAATTCTTTTCGCTCTCT
droEug1	scf7180000407756:20194-20367 +		TTCAAGT--CTGGGACACTTCAA----------T-TTTT-TCAATTTTTCGCAA-ATTTTGATGATGG-----TACCCCTTATAAA-AATGCGA-AATTTGGG-CA-AAAATTAATTTTCTCAAAATCGAAAAACGAGTGATA------------GGGGTGGTAAGTG--ATG-GTCGTTAGCAGCA---CAAAATAGTCGTTCTCTTGGCTGTAA
droBia1	scf7180000300910:2355784-2355946 -		TTCAAAT--CTAAAAAAGTTAAA----------A-TTTT-GCTATTTTTTGCAA-AAAATGATGATGG-----TACCCCTTA---AAAAATCGA-AAATTTTTCAA-AAAATTTATTTTT-CAA---------GAAAGTGATG------------GGGATAGTTAGCG--TAA-GATGTGAGCTGCT---CAAAACTGTCGGTCTTTTGTCTGTAC
droTak1	scf7180000414222:84361-84535 +		TTCAAAT--CTGGAAATTTTTAA----------T-TTTT-GCCATTTTTCGCAA-AAAATGATGATGG-----TACCCCTTATCAAAAATGCGA-AAATTGGGTCC-AAAATTAATTTTC-TCAAATCAGTCGGAAAGTGCGC------------T--GATATTAATATTTAG-GTTGTAAGCTGCA---CAAAACAGTGCGCCTTTTTGATTTGG
droEle1	scf7180000491273:2144372-2144542 -		TTCAAAT--CTAAAAAAAAAAAA----------A-ATTT-TGATTGTTTAGCAA-AAAATTATGATGT-----TACCCCTTATAAAAAAATCGA-AAAATGGGCAA-AATTTTAATTATT-CAAAATTAGCCAAAAAGTGATG------------AGGGTCGTTAGTT--TAG-CTTGTCAGCTGTG---TAGAACAGTCTTTCTTTTAGTT----
droRho1	scf7180000779175:54219-54383 +		TTCACAG--CTAAAATTTTGTAT----------T-TTTG-TCCAATTTTCGCAA-ATTTTGATGATGT-----TACCCCTTACAAAAAATGCGA-AATTTGGA-CA-AAAAATAATTTTT-CAAAATTTGA---------GCA------------CGGATCGATAGTT--TAG-GTCACCAGCTGCA---CAAAACAGTCATTCTTTTCGCTCTAC
droFic1	scf7180000454105:1936098-1936273 -		TTCAAAT--CTGAAAATTTTTAG----------T-TTTG-GCCATTTTTCGCAA-AAAATGATGATGT-----AACCCCTTACCAAAAATGCGACAAAATGGC-CAAAAAATGAATTTTT-CGAAGTCTGCCAAAAAGTGATT------------GGGGTCGTTAGCC--TAG-GTGCTAAGCTGCA---AAAAACAGTCATTCTTTTAGTTCTAT
droKik1	scf7180000302706:78081-78269 +		TACCAAA--TTTAAAATTTTAAGAAATCAAAA-TTTTTG-GCGA-TTTTTGCTA-ATTTTAATGATGT-----AACCCCTTATAAAATTTTGCA-AAAATGGCAAA-AAAATCGATTTTTTCCAGACATGTCTAGAAA-GATTCGCATGTTAATGCTGA------------TC-AAGAATATGGGTTTATGGGGTCGGAAATGATTTCTTCACTTA
droAna3	scaffold_13334:557799-557973 -		TCATTAGATTTTGAAAGTCAAACAAATCAA---A-TTTT-TCGATTTTTTTCAACATTTTTATGATGG-----TACCCCTTGCAAAATTTTCGA-AAATCGGCTCA-GATTTTAGTTTTC-TAGATTTTG----------ATA------------GGGAATGATACTA--CTC-GATGATCTAAGAT---CGGGAAGGTATGCCATTTTGAAATCG
droBip1	scf7180000396708:2849559-2849733 +		TCAAAAT--ATTGCATAAATGGATTTTGCAAATT-TTCGTTCGATTTTTGGCCA-AAA-TTGTGATGT-----TACCCCTTTGAAAAATTTCAA-AAATTGGGTCA-GATTTTAATTTAT-GAGATTTTTAGTAAAAATTAAC------------ATAC------------TC-AAAAATGTAAGTT---CGGGAAGGCATACCATTCACCATTCC
droWil2	scf2_1100000006624:643-820 +		TTCAACA--ATGGAAATTAAATT----------T-TTTG-GCCATGTTTTACAA-GTTTTAATGACTCCCCTCATCCCCTTACAAAAAATGCGT-AAATTGAC-CC-AAAACTAATTTCC-CTAAAT-ATTCAAAAAGTAATA------------GGGATCGTTAGCG--CTG-GTAATTAGTTTCT---CAAAACAGTTATTCTTACTTCTATAA

Species

Coordinate

Alignment

droSim3

chrU_M_2695:832-1007 -

dsi_180

TTCAAGT--TTTGGAATTCTGAG----------A-GGTT-TCAATTTTTTGCAA-ATTTTGATGATGG-----TACCCCTTACAAAAAATTCGA-AAATTTGGCCA-AAAATTAATTTTA-CAAAATCAGTTTAAAAGTGAAA------------GGGGATGTTAGTA--TTGTATTGTAAGGAGTA---CAAAATGGTACTCCTTTTTGCTCTCT

droSec2

scaffold_4622:770-944 +

TTCAAGT--TTTGGAATTCTAGG----------A-GGTT-TCAATTTTTTGCAA-ATTTTGATGATGG-----TACCCCTTACAAAAAATTCGA-AAATTTGGCCA-AAAATTAATTTTA-CAAAATCAGTTTAAAAGTGAAA------------GGGGTTGTTAGTA--TTG-GTTGTAAGGAGTA---CAAAATGGTACTCCTTTTTGCTCTCT

dm3

chrX:8721644-8721818 +

TTCAAGT--TTTGGAGTTCTAGG----------A-TTTT-TCAATTTTTTGCAA-ATTTTGATGATGT-----TACCCCTTACAAAAAATGCGA-AAATTTGGCCA-AAAATTAATTTTA-CAAAATCAGTTTAAAAGTGAAA------------GGGGTTGTTAGTA--TTG-GTTGTAAGGAGTA---TAAAATGGTACTACTTTTTGCTCTGT

droEre2

scaffold_4690:3253882-3254056 +

TTCAAAT--TTGGAAATTTTAAT----------T-TTTT-TCAATTTTTTGCAA-ATTTTGATGATGG-----TACCCCTTACAAAAAATGCGA-AAATTGACCAA-AAAATTGATTTTC-CCAACTCGGTCAAAAAGTGATA------------GGGATGTTTAGTA--TTG-GTAATTAGGAGCA---CAAAACGGTAATTACTTTATCTGTGC

droYak3

X:7880819-7880993 +

TTTTCAA--AAAAAAATTCTATG----------T-CGTT-CCAATTTTTTGCAA-ATTTTGATGATGG-----TACCCCTTACAAAAAATGCGA-AAAAATGGCCA-AAAATTATTTTAA-CACAGTCGGTTAAAAAGTGATT------------TGGTTGGTTAGTA--TTG-GTAATTAGGAGTA---AAAAACTGTAATTCTTTTCGCTCTCT

droEug1

scf7180000407756:20194-20367 +

TTCAAGT--CTGGGACACTTCAA----------T-TTTT-TCAATTTTTCGCAA-ATTTTGATGATGG-----TACCCCTTATAAA-AATGCGA-AATTTGGG-CA-AAAATTAATTTTCTCAAAATCGAAAAACGAGTGATA------------GGGGTGGTAAGTG--ATG-GTCGTTAGCAGCA---CAAAATAGTCGTTCTCTTGGCTGTAA

droBia1

scf7180000300910:2355784-2355946 -

TTCAAAT--CTAAAAAAGTTAAA----------A-TTTT-GCTATTTTTTGCAA-AAAATGATGATGG-----TACCCCTTA---AAAAATCGA-AAATTTTTCAA-AAAATTTATTTTT-CAA---------GAAAGTGATG------------GGGATAGTTAGCG--TAA-GATGTGAGCTGCT---CAAAACTGTCGGTCTTTTGTCTGTAC

droTak1

scf7180000414222:84361-84535 +

TTCAAAT--CTGGAAATTTTTAA----------T-TTTT-GCCATTTTTCGCAA-AAAATGATGATGG-----TACCCCTTATCAAAAATGCGA-AAATTGGGTCC-AAAATTAATTTTC-TCAAATCAGTCGGAAAGTGCGC------------T--GATATTAATATTTAG-GTTGTAAGCTGCA---CAAAACAGTGCGCCTTTTTGATTTGG

droEle1

scf7180000491273:2144372-2144542 -

TTCAAAT--CTAAAAAAAAAAAA----------A-ATTT-TGATTGTTTAGCAA-AAAATTATGATGT-----TACCCCTTATAAAAAAATCGA-AAAATGGGCAA-AATTTTAATTATT-CAAAATTAGCCAAAAAGTGATG------------AGGGTCGTTAGTT--TAG-CTTGTCAGCTGTG---TAGAACAGTCTTTCTTTTAGTT----

droRho1

scf7180000779175:54219-54383 +

TTCACAG--CTAAAATTTTGTAT----------T-TTTG-TCCAATTTTCGCAA-ATTTTGATGATGT-----TACCCCTTACAAAAAATGCGA-AATTTGGA-CA-AAAAATAATTTTT-CAAAATTTGA---------GCA------------CGGATCGATAGTT--TAG-GTCACCAGCTGCA---CAAAACAGTCATTCTTTTCGCTCTAC

droFic1

scf7180000454105:1936098-1936273 -

TTCAAAT--CTGAAAATTTTTAG----------T-TTTG-GCCATTTTTCGCAA-AAAATGATGATGT-----AACCCCTTACCAAAAATGCGACAAAATGGC-CAAAAAATGAATTTTT-CGAAGTCTGCCAAAAAGTGATT------------GGGGTCGTTAGCC--TAG-GTGCTAAGCTGCA---AAAAACAGTCATTCTTTTAGTTCTAT

droKik1

scf7180000302706:78081-78269 +

TACCAAA--TTTAAAATTTTAAGAAATCAAAA-TTTTTG-GCGA-TTTTTGCTA-ATTTTAATGATGT-----AACCCCTTATAAAATTTTGCA-AAAATGGCAAA-AAAATCGATTTTTTCCAGACATGTCTAGAAA-GATTCGCATGTTAATGCTGA------------TC-AAGAATATGGGTTTATGGGGTCGGAAATGATTTCTTCACTTA

droAna3

scaffold_13334:557799-557973 -

TCATTAGATTTTGAAAGTCAAACAAATCAA---A-TTTT-TCGATTTTTTTCAACATTTTTATGATGG-----TACCCCTTGCAAAATTTTCGA-AAATCGGCTCA-GATTTTAGTTTTC-TAGATTTTG----------ATA------------GGGAATGATACTA--CTC-GATGATCTAAGAT---CGGGAAGGTATGCCATTTTGAAATCG

droBip1

scf7180000396708:2849559-2849733 +

TCAAAAT--ATTGCATAAATGGATTTTGCAAATT-TTCGTTCGATTTTTGGCCA-AAA-TTGTGATGT-----TACCCCTTTGAAAAATTTCAA-AAATTGGGTCA-GATTTTAATTTAT-GAGATTTTTAGTAAAAATTAAC------------ATAC------------TC-AAAAATGTAAGTT---CGGGAAGGCATACCATTCACCATTCC

droWil2

scf2_1100000006624:643-820 +

TTCAACA--ATGGAAATTAAATT----------T-TTTG-GCCATGTTTTACAA-GTTTTAATGACTCCCCTCATCCCCTTACAAAAAATGCGT-AAATTGAC-CC-AAAACTAATTTCC-CTAAAT-ATTCAAAAAGTAATA------------GGGATCGTTAGCG--CTG-GTAATTAGTTTCT---CAAAACAGTTATTCTTACTTCTATAA

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droSim3

TTCAAGT--TTTGGAATTCTGAG----------A-GGTT-TCAATTTTTTGCAA-ATTTTGATGATGG-----TACCCCTTACAAAAAATTCGA-AAATTTGGCCA-AAAATTAATTTTA-CAAAATCAGTTTAAAAGTGAAA------------GGGGATGTTAGTA--TTGTATTGTAAGGAGTA---CAAAATGGTACTCCTTTTTGCTCTCT	Size	Hit Count	Total Norm	Total	V044 Embryo	M023 Head	M024 Male-body	M025 Embryo	O001 Testis	O002 Head	M053 Female-body	SRR553485 Ovary	SRR553486 Ovary	SRR553487 Ovary	SRR553488 Ovary	SRR618934 Ovary
..............................................................................................................................TCAGTTTAAAAGTGAAA------------GGGGA........................................................	22	2	17.50	35	0	0	0	0	0	0	0	3	19	6	7	0
...............................................................................................................................CAGTTTAAAAGTGAAA------------GGGGA........................................................	21	2	10.00	20	0	0	0	0	20	0	0	0	0	0	0	0
...............................................................................................................................CAGTTTAAAAGTGAAA------------GGGGATG......................................................	23	2	6.00	12	0	0	0	0	12	0	0	0	0	0	0	0
..............................................................................................................................................................GATGTTAGTA--TTGTATTGTAAGGAG...............................	25	1	2.00	2	0	0	0	0	0	0	0	0	1	1	0	0
..........................................................................................................................AAAATCAGTTTAAAAGTGAAA------------GGGGA........................................................	26	2	1.50	3	0	0	0	0	0	0	0	0	3	0	0	0
..............................................................................................................................................................GATGTTAGTA--TTGTATTGTAAGGAGT..............................	26	1	1.00	1	0	0	0	0	0	0	0	0	1	0	0	0
...........................................................................................................................AAATCAGTTTAAAAGTGAAA------------GGGGA........................................................	25	2	1.00	2	0	0	0	0	0	0	0	1	1	0	0	0
...............................................................................................................................CAGTTTAAAAGTGAAA------------GGGGAT.......................................................	22	2	1.00	2	0	0	0	0	2	0	0	0	0	0	0	0
...............................................................................................................................CAGTTTAAAAGTGAAA------------GGGGATGTTA...................................................	26	2	1.00	2	0	0	0	0	2	0	0	0	0	0	0	0
.....................................................................................................................TTA-CAAAATCAGTTTAAAAGTGAAA------------GGGGA........................................................	30	1	1.00	1	0	0	0	0	0	0	0	0	1	0	0	0
............................................................................................................................AATCAGTTTAAAAGTGAAA------------GGGGA........................................................	24	2	1.00	2	0	0	0	0	0	2	0	0	0	0	0	0
.........................................................................................................................CAAAATCAGTTTAAAAGTGAAA------------GGGGA........................................................	27	2	1.00	2	0	0	0	0	0	0	0	0	2	0	0	0
......................................................................................................................TA-CAAAATCAGTTTAAAAGTGAAA------------GGGGA........................................................	29	1	1.00	1	0	0	0	0	0	0	0	0	1	0	0	0
...................................................................................................................................TTAAAAGTGAAA------------GGGGA........................................................	17	2	0.50	1	0	0	0	0	0	0	0	0	1	0	0	0
.....................................................................................................................................AAAAGTGAAA------------GGGGATGTTAGTA--TT............................................	25	2	0.50	1	0	0	0	0	0	0	0	0	0	0	0	1
.............................................................................................................................ATCAGTTTAAAAGTGAAA------------GGGGA........................................................	23	2	0.50	1	0	0	0	0	0	0	0	0	1	0	0	0
TTCAAGT--TTTGGAATTCTGAG----------A-GGTT-................................................................................................................................................................................	26	18	0.39	7	0	0	0	0	0	0	0	7	0	0	0	0
....................................................................................................................TTTA-CAAAATCAGTTTAAAAGTGAA..........................................................................	25	20	0.35	7	0	0	0	0	0	0	0	0	0	7	0	0
.........................................................................TACCCCTTACAAAAAATTCGA-AAATT....................................................................................................................	26	20	0.30	6	0	0	0	0	0	0	0	0	0	6	0	0
..CAAGT--TTTGGAATTCTGAG----------A-GGTT-................................................................................................................................................................................	24	18	0.22	4	0	0	0	0	0	0	0	1	0	3	0	0
...........................................................................................................................................................................TGTATTGTAAGGAGTA---CA........................	18	7	0.14	1	0	0	0	0	0	0	0	0	0	0	0	1
TTCAAGT--TTTGGAATTCTGAG----------A-GGT..................................................................................................................................................................................	25	18	0.11	2	0	0	0	0	0	0	0	2	0	0	0	0
.TCAAGT--TTTGGAATTCTGAG----------A-GGTT-................................................................................................................................................................................	25	18	0.11	2	0	0	0	0	0	0	0	1	0	1	0	0
.....................................................................................................................TTA-CAAAATCAGTTTAAAAGTGAAA------------GG...........................................................	27	20	0.10	2	0	0	0	0	0	0	0	0	2	0	0	0
...............................................TTTGCAA-ATTTTGATGATGG-----TACCC..........................................................................................................................................	25	20	0.10	2	0	0	0	0	0	0	0	2	0	0	0	0
TTCAAGT--TTTGGAATTCTGAG----------A-GGTT-TCAA............................................................................................................................................................................	30	18	0.06	1	0	0	0	0	0	0	0	0	1	0	0	0
..CAAGT--TTTGGAATTCTGAG----------A-GGTT-TC..............................................................................................................................................................................	26	18	0.06	1	0	0	0	0	0	0	0	0	0	1	0	0
.TCAAGT--TTTGGAATTCTGAG----------A-GGTT-TCAA............................................................................................................................................................................	29	18	0.06	1	0	0	0	0	0	0	0	1	0	0	0	0
.TCAAGT--TTTGGAATTCTGAG----------A-GGT..................................................................................................................................................................................	24	18	0.06	1	0	0	0	0	0	0	0	1	0	0	0	0
..CAAGT--TTTGGAATTCTGAG----------A-GGTT-TCAA............................................................................................................................................................................	28	18	0.06	1	0	0	0	0	0	0	0	0	0	1	0	0
...AAGT--TTTGGAATTCTGAG----------A-GGTT-................................................................................................................................................................................	23	18	0.06	1	0	0	0	0	0	0	0	0	0	1	0	0
TTCAAGT--TTTGGAATTCTGAG----------A-GG...................................................................................................................................................................................	24	18	0.06	1	0	0	0	0	0	0	0	1	0	0	0	0
...AAGT--TTTGGAATTCTGAG----------A-.....................................................................................................................................................................................	19	19	0.05	1	0	0	0	0	0	0	0	0	0	0	0	1
.......................................................................................................................A-CAAAATCAGTTTAAAAGT.............................................................................	19	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	1
.........................................................................TACCCCTTACAAAAAAT..............................................................................................................................	17	20	0.05	1	0	0	0	0	0	0	0	0	1	0	0	0
.........................................................................TACCCCTTACAAAAAATTCGA-AAA......................................................................................................................	24	20	0.05	1	0	0	0	0	0	0	0	1	0	0	0	0
.....................................................................................................................TTA-CAAAATCAGTTTAAAAG..............................................................................	20	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	1
...............................................................................................................................CAGTTTAAAAGTGAAA------------GGG..........................................................	19	20	0.05	1	0	0	0	0	0	0	0	0	1	0	0	0
......................................T-TCAATTTTTTGCAA-ATTTTGATGA.......................................................................................................................................................	25	20	0.05	1	0	0	0	0	0	0	0	1	0	0	0	0
...........................................ATTTTTTGCAA-ATTTTGATGATGG-----...............................................................................................................................................	24	20	0.05	1	0	0	0	0	0	0	0	0	1	0	0	0

droSec2

TTCAAGT--TTTGGAATTCTAGG----------A-GGTT-TCAATTTTTTGCAA-ATTTTGATGATGG-----TACCCCTTACAAAAAATTCGA-AAATTTGGCCA-AAAATTAATTTTA-CAAAATCAGTTTAAAAGTGAAA------------GGGGTTGTTAGTA--TTG-GTTGTAAGGAGTA---CAAAATGGTACTCCTTTTTGCTCTCT	Size	Hit Count	Total Norm	Total	V113 Male-body	V114 Embryo	V115 Head	M054 Female-body
................................................................................................................................................................TGTTAGTA--TTG-GTTGTAAGGAGTA---CAA.......................	27	20	0.25	5	5	0	0	0
...........................................................TGATGATGG-----TACCCCTTACAAAAAATT.............................................................................................................................	27	20	0.20	4	0	0	0	4
...................................................................................................................TTTTA-CAAAATCAGTTTAAAAGTGAAA------------G............................................................	28	20	0.10	2	0	2	0	0
......T--TTTGGAATTCTAGG----------A-GGTT-T...............................................................................................................................................................................	21	20	0.05	1	0	1	0	0
.................................................................................................................................................................GTTAGTA--TTG-GTTGTAAGGAG...............................	21	20	0.05	1	0	0	1	0
..........................................................................................................................................TGAAA------------GGGGTTGTTAGTA--TTG-G.........................................	22	20	0.05	1	1	0	0	0
...............................................................GATGG-----TACCCCTTACAAAAAATTCG...........................................................................................................................	25	20	0.05	1	0	0	0	1
...................TAGG----------A-GGTT-TCAATTTTTTGCAA-ATT..............................................................................................................................................................	26	20	0.05	1	0	1	0	0
..............................................................................................................................TCAGTTTAAAAGTGAAA------------GGGGTTGT.....................................................	25	20	0.05	1	0	1	0	0
..........................................................................................................................................................................TTG-GTTGTAAGGAGTA---CAAAATGGTAC...............	27	20	0.05	1	1	0	0	0
...................................................................................................................TTTTA-CAAAATCAGTTTAAAAGT.............................................................................	23	20	0.05	1	0	1	0	0
...........................................................................CCCCTTACAAAAAATTCGA-AAATT....................................................................................................................	24	20	0.05	1	0	0	0	1
........................................TCAATTTTTTGCAA-ATTTTGATGATGG-----T..............................................................................................................................................	28	20	0.05	1	0	0	0	1
....................................................................................................................................TAAAAGTGAAA------------GGGGTTGTTAGTA--..............................................	24	20	0.05	1	0	0	0	1
.............................................................................CCTTACAAAAAATTCGA-AAATTTGG.................................................................................................................	25	20	0.05	1	0	0	0	1
................................................................................................................TAATTTTA-CAAAATCAGTTTAAAAG..............................................................................	25	20	0.05	1	0	0	0	1
................................................................................................................................................................TGTTAGTA--TTG-GTTGTAAGG.................................	20	20	0.05	1	1	0	0	0
................TTCTAGG----------A-GGTT-TCAATTTTTTGCAA-.................................................................................................................................................................	26	20	0.05	1	0	1	0	0

droEre2

TTCAAAT--TTGGAAATTTTAAT----------T-TTTT-TCAATTTTTTGCAA-ATTTTGATGATGG-----TACCCCTTACAAAAAATGCGA-AAATTGACCAA-AAAATTGATTTTC-CCAACTCGGTCAAAAAGTGATA------------GGGATGTTTAGTA--TTG-GTAATTAGGAGCA---CAAAACGGTAATTACTTTATCTGTGC	Size	Hit Count	Total Norm	Total	V040 Embryo	V060 Head	V107 Male-body	V108 Head	M055 Female-body	M057 Embryo
......................................................................................................CCAA-AAAATTGATTTTC-CCAA...........................................................................................	21	20	0.05	1	0	0	0	0	1	0
..................................................................................................................................................................TTAGTA--TTG-GTAATTAGGAGC..............................	21	20	0.05	1	0	0	1	0	0	0
................................................TTGCAA-ATTTTGATGATGG-----TAC............................................................................................................................................	22	20	0.05	1	0	0	0	0	1	0
............................................................................................................AAATTGATTTTC-CCAACTCGG......................................................................................	21	20	0.05	1	0	0	0	0	1	0
...................................................................................................................................................................TAGTA--TTG-GTAATTAGGA................................	18	20	0.05	1	0	0	0	1	0	0

droYak3

TTTTCAA--AAAAAAATTCTATG----------T-CGTT-CCAATTTTTTGCAA-ATTTTGATGATGG-----TACCCCTTACAAAAAATGCGA-AAAAATGGCCA-AAAATTATTTTAA-CACAGTCGGTTAAAAAGTGATT------------TGGTTGGTTAGTA--TTG-GTAATTAGGAGTA---AAAAACTGTAATTCTTTTCGCTCTCT	Size	Hit Count	Total Norm	Total	V046 Embryo	V052 Head	V120 Male-body	V058 Head	M026 Head	M043 Female-body	M056 Embryo
..............................................................................................................................................................TTGGTTAGTA--TTG-GTAATTAGGAG...............................	24	20	0.35	7	0	0	1	0	0	6	0
..........................................................TTGATGATGG-----TACCCCTTACAAAAAA...............................................................................................................................	26	20	0.10	2	0	0	0	0	0	2	0
..............................................................................................................................................................TTGGTTAGTA--TTG-GTAATTAGGA................................	23	20	0.05	1	0	0	0	0	0	0	1
.................................................TGCAA-ATTTTGATGATGG-----TACC...........................................................................................................................................	22	20	0.05	1	0	0	0	0	0	0	1
..............................................TTTTGCAA-ATTTTGATGATGG-----TACCC..........................................................................................................................................	26	20	0.05	1	0	0	0	0	0	0	1
..................................................................GG-----TACCCCTTACAAAAAATGCGA-AAA......................................................................................................................	26	20	0.05	1	0	0	0	0	0	1	0
............................................TTTTTTGCAA-ATTTTGATGATGG-----TAC............................................................................................................................................	26	20	0.05	1	0	0	0	0	0	1	0
..........................................................................................................................................................................TTG-GTAATTAGGAGTA---AAAAACTGTA................	26	20	0.05	1	0	0	0	0	0	1	0
..............AATTCTATG----------T-CGTT-CCAATTTTTTGC....................................................................................................................................................................	26	20	0.05	1	0	0	0	0	0	1	0

droEug1

TTCAAGT--CTGGGACACTTCAA----------T-TTTT-TCAATTTTTCGCAA-ATTTTGATGATGG-----TACCCCTTATAAA-AATGCGA-AATTTGGG-CA-AAAATTAATTTTCTCAAAATCGAAAAACGAGTGATA------------GGGGTGGTAAGTG--ATG-GTCGTTAGCAGCA---CAAAATAGTCGTTCTCTTGGCTGTAA	Size	Hit Count	Total Norm	Total
No Reads

droBia1

TTCAAAT--CTAAAAAAGTTAAA----------A-TTTT-GCTATTTTTTGCAA-AAAATGATGATGG-----TACCCCTTA---AAAAATCGA-AAATTTTTCAA-AAAATTTATTTTT-CAA---------GAAAGTGATG------------GGGATAGTTAGCG--TAA-GATGTGAGCTGCT---CAAAACTGTCGGTCTTTTGTCTGTAC	Size	Hit Count	Total Norm	Total
No Reads

droTak1

TTCAAAT--CTGGAAATTTTTAA----------T-TTTT-GCCATTTTTCGCAA-AAAATGATGATGG-----TACCCCTTATCAAAAATGCGA-AAATTGGGTCC-AAAATTAATTTTC-TCAAATCAGTCGGAAAGTGCGC------------T--GATATTAATATTTAG-GTTGTAAGCTGCA---CAAAACAGTGCGCCTTTTTGATTTGG	Size	Hit Count	Total Norm	Total
No Reads

droEle1

TTCAAAT--CTAAAAAAAAAAAA----------A-ATTT-TGATTGTTTAGCAA-AAAATTATGATGT-----TACCCCTTATAAAAAAATCGA-AAAATGGGCAA-AATTTTAATTATT-CAAAATTAGCCAAAAAGTGATG------------AGGGTCGTTAGTT--TAG-CTTGTCAGCTGTG---TAGAACAGTCTTTCTTTTAGTT----	Size	Hit Count	Total Norm	Total
No Reads

droRho1

TTCACAG--CTAAAATTTTGTAT----------T-TTTG-TCCAATTTTCGCAA-ATTTTGATGATGT-----TACCCCTTACAAAAAATGCGA-AATTTGGA-CA-AAAAATAATTTTT-CAAAATTTGA---------GCA------------CGGATCGATAGTT--TAG-GTCACCAGCTGCA---CAAAACAGTCATTCTTTTCGCTCTAC	Size	Hit Count	Total Norm	Total
No Reads

droFic1

TTCAAAT--CTGAAAATTTTTAG----------T-TTTG-GCCATTTTTCGCAA-AAAATGATGATGT-----AACCCCTTACCAAAAATGCGACAAAATGGC-CAAAAAATGAATTTTT-CGAAGTCTGCCAAAAAGTGATT------------GGGGTCGTTAGCC--TAG-GTGCTAAGCTGCA---AAAAACAGTCATTCTTTTAGTTCTAT	Size	Hit Count	Total Norm	Total
No Reads

droKik1

TACCAAA--TTTAAAATTTTAAGAAATCAAAA-TTTTTG-GCGA-TTTTTGCTA-ATTTTAATGATGT-----AACCCCTTATAAAATTTTGCA-AAAATGGCAAA-AAAATCGATTTTTTCCAGACATGTCTAGAAA-GATTCGCATGTTAATGCTGA------------TC-AAGAATATGGGTTTATGGGGTCGGAAATGATTTCTTCACTTA	Size	Hit Count	Total Norm	Total
No Reads

droAna3

TCATTAGATTTTGAAAGTCAAACAAATCAA---A-TTTT-TCGATTTTTTTCAACATTTTTATGATGG-----TACCCCTTGCAAAATTTTCGA-AAATCGGCTCA-GATTTTAGTTTTC-TAGATTTTG----------ATA------------GGGAATGATACTA--CTC-GATGATCTAAGAT---CGGGAAGGTATGCCATTTTGAAATCG	Size	Hit Count	Total Norm	Total	V105 Male-body	V106 Head	V055 Head	V039 Embryo	M044 Female-body	M058 Embryo
No Reads

droBip1

TCAAAAT--ATTGCATAAATGGATTTTGCAAATT-TTCGTTCGATTTTTGGCCA-AAA-TTGTGATGT-----TACCCCTTTGAAAAATTTCAA-AAATTGGGTCA-GATTTTAATTTAT-GAGATTTTTAGTAAAAATTAAC------------ATAC------------TC-AAAAATGTAAGTT---CGGGAAGGCATACCATTCACCATTCC	Size	Hit Count	Total Norm	Total
No Reads

droWil2

TTCAACA--ATGGAAATTAAATT----------T-TTTG-GCCATGTTTTACAA-GTTTTAATGACTCCCCTCATCCCCTTACAAAAAATGCGT-AAATTGAC-CC-AAAACTAATTTCC-CTAAAT-ATTCAAAAAGTAATA------------GGGATCGTTAGCG--CTG-GTAATTAGTTTCT---CAAAACAGTTATTCTTACTTCTATAA	Size	Hit Count	Total Norm	Total	V117 Male-body	V118 Embryo	V119 Head	M020 Head	M045 Female-body
..........................................................................................................................................................................CTG-GTAATTAGTTTCT---CAA.......................	19	1	1.00	1	0	1	0	0	0
..........................................................................................................................................................................CTG-GTAATTAGTTTCT---CAAAACAGT.................	25	1	1.00	1	0	1	0	0	0

dsi_180

chrU_M_2695:882-957 -

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-11.7	-11.3