dsi_170

ACAGGGTATACGAACCAATTCAAATATTCTTTCAGATTATATGACTTTAACGTATACTTTTCCGGGTTGGTGGTTGAACTTCGATTTTAAGCATTTAAATAAGATAAATCTGTTCATTCTTCGACCCAAAAAGTTCACGTGAAGAAGCAGCGAACATCAAATGTGTGAATGTAAACTCAATTGTCACTGGTCGTG ********************************************...((((..((((((..(((((((.((.(((((...(((((..................))))).))))).)).))))))).))))))..))))....*************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	O001 Testis	M024 male body	M023 head	O002 Head	SRR553485 Ovary	SRR553488 Ovary	SRR553487 Ovary	SRR553486 Ovary	M025 embryo	M053 Female-body	SRR618934 Ovary
............................................................................................................TCTGTTCATTCTTCGACCCAA..................................................................	21	0	1	898.00	898	678	200	7	8	0	2	1	2	0	0	0
............................................................................................................TCTGTTCATTCTTCGACCCAAA.................................................................	22	0	1	467.00	467	182	272	3	1	4	3	1	0	1	0	0
...........................................................................................................ATCTGTTCATTCTTCGACCCA...................................................................	21	0	1	302.00	302	281	15	0	4	0	0	0	0	0	1	1
...........................................................................................................ATCTGTTCATTCTTCGACCCAA..................................................................	22	0	1	273.00	273	258	7	0	6	0	1	1	0	0	0	0
............................................................................................................TCTGTTCATTCTTCGACCC....................................................................	19	0	1	223.00	223	208	9	1	5	0	0	0	0	0	0	0
............................................................................................................TCTGTTCATTCTTCGACCCA...................................................................	20	0	1	216.00	216	132	66	17	1	0	0	0	0	0	0	0
...........................................................................................................ATCTGTTCATTCTTCGACCC....................................................................	20	0	1	108.00	108	102	3	0	3	0	0	0	0	0	0	0
...........................................................................................................ATCTGTTCATTCTTCGACCCAAA.................................................................	23	0	1	95.00	95	88	5	0	1	1	0	0	0	0	0	0
..........................................................................................................AATCTGTTCATTCTTCGACCCA...................................................................	22	0	1	60.00	60	45	12	0	0	1	0	1	1	0	0	0
............................................................................................................TCTGTTCATTCTTCGACC.....................................................................	18	0	1	44.00	44	35	6	3	0	0	0	0	0	0	0	0
...........................................................................................................ATCTGTTCATTCTTCGACC.....................................................................	19	0	1	33.00	33	30	2	1	0	0	0	0	0	0	0	0
............................................................................................................TCTGTTCATTCTTCGACCCAAAA................................................................	23	0	1	26.00	26	14	12	0	0	0	0	0	0	0	0	0
..........................................................................................................AATCTGTTCATTCTTCGACCC....................................................................	21	0	1	24.00	24	12	11	1	0	0	0	0	0	0	0	0
...........................................................................................................ATCTGTTCATTCTTCGAC......................................................................	18	0	1	9.00	9	9	0	0	0	0	0	0	0	0	0	0
.............................................................................................................CTGTTCATTCTTCGACCCAA..................................................................	20	0	1	7.00	7	6	1	0	0	0	0	0	0	0	0	0
..........................................................................................................AATCTGTTCATTCTTCGACC.....................................................................	20	0	1	7.00	7	6	1	0	0	0	0	0	0	0	0	0
..........................................................................................................AATCTGTTCATTCTTCGACCCAA..................................................................	23	0	1	6.00	6	4	2	0	0	0	0	0	0	0	0	0
.............................................................................................................CTGTTCATTCTTCGACCCA...................................................................	19	0	1	6.00	6	6	0	0	0	0	0	0	0	0	0	0
.............................................................CCGGGTTGGTGGTTGAACTTCGATTT............................................................................................................	26	0	1	6.00	6	6	0	0	0	0	0	0	0	0	0	0
.............................................................CCGGGTTGGTGGTTGAACTTCGAT..............................................................................................................	24	0	1	6.00	6	6	0	0	0	0	0	0	0	0	0	0
..........................................................................................................AATCTGTTCATTCTTCGAC......................................................................	19	0	1	4.00	4	2	2	0	0	0	0	0	0	0	0	0
...........................................................................................................ATCTGTTCATTCTTCGACCCAAAA................................................................	24	0	1	4.00	4	4	0	0	0	0	0	0	0	0	0	0
..........................................................................................................AATCTGTTCATTCTTCGACCCT...................................................................	22	1	1	4.00	4	0	2	0	0	0	0	0	0	2	0	0
............................................................................................................TCTGTTCATTCTTCGACCCAG..................................................................	21	1	1	4.00	4	0	4	0	0	0	0	0	0	0	0	0
.............................................................CCGGGTTGGTGGTTGAACTTC.................................................................................................................	21	0	1	3.00	3	3	0	0	0	0	0	0	0	0	0	0
............................................................................................................TCTGTTCATTCTTCGACCCG...................................................................	20	1	1	3.00	3	0	3	0	0	0	0	0	0	0	0	0
.............................................................................................................CTGTTCATTCTTCGACCCAAA.................................................................	21	0	1	2.00	2	2	0	0	0	0	0	0	0	0	0	0
.............................................................CCGGGTTGGTGGTTGAACTTCG................................................................................................................	22	0	1	2.00	2	1	0	0	0	1	0	0	0	0	0	0
..........................................................................................................AATCTGTTCATTCTTCGA.......................................................................	18	0	1	2.00	2	2	0	0	0	0	0	0	0	0	0	0
............................................................................................................TCTGTTCATTCTTCGACCCACC.................................................................	22	2	1	2.00	2	0	2	0	0	0	0	0	0	0	0	0
............................................................................................................TCTGTTCATTCTTCGACCCT...................................................................	20	1	1	2.00	2	0	2	0	0	0	0	0	0	0	0	0
.............................................................................................................CTGTTCATTCTTCGACCC....................................................................	18	0	1	2.00	2	2	0	0	0	0	0	0	0	0	0	0
.......................................................................................TAAGCATTTAAATAAGATAA........................................................................................	20	0	1	2.00	2	2	0	0	0	0	0	0	0	0	0	0
............................................................................................................TCTGTTCATTCTTCGACCCAAT.................................................................	22	1	1	2.00	2	0	2	0	0	0	0	0	0	0	0	0
..........................................................................................................AATCTGTTCATTCTTCGACTC....................................................................	21	1	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
............................................................................................................TCTGTTCATTCTTCGACCCAC..................................................................	21	1	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
............................................................................................................TCTGTTCATTATTCGACCC....................................................................	19	1	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
...............................................................................................................GTTCATTCTTCGACCCAA..................................................................	18	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0
............................................................................................................TCTGTTCATTCTTCTACCCAAA.................................................................	22	1	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
...........................................................................................................ATCCGTTCATTCTTCGACCCA...................................................................	21	1	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
............................................................................................................TATGTTCATTCTTCGACCCAAA.................................................................	22	1	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
.............................................................CCGGGTTGGTGGTTGAACTTCGATT.............................................................................................................	25	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0
...........................................................................................................ATCTGTTCATTCTTCGACCCT...................................................................	21	1	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
............................................................................................................TCCGTTCATTCTTCGACCCAAA.................................................................	22	1	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
............................................................................................................TTTGTTCATTCTTCGACCCAA..................................................................	21	1	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
............................................................................................................TCTGATCATTCTTCGACCCAAA.................................................................	22	1	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
............................................................TCCGGGTTGGTGGTTGAACTTCGAT..............................................................................................................	25	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0
............................................................................................................TCTGTTCATTCTTCCACCCAAA.................................................................	22	1	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
............................................................................................................TCTGTTCATTCTTCGACCCAAG.................................................................	22	1	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
................................................................................................................TTCATTCTTCGACCCAAA.................................................................	18	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0
............................................................................................................TCTGTTCATTCTTCGGCCCAAA.................................................................	22	1	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
............................................................................................................TCTTTTCATTCTTCGACCCAAA.................................................................	22	1	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
............................................................................................................TCTGTTGATTCTTCGACCCAAA.................................................................	22	1	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
...........................................................................................................ATCTGTTCATTCTTCAACCCA...................................................................	21	1	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
...............................................................GGGTTGGTGGTTGAACTTCGATT.............................................................................................................	23	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0
..........................................................................................................AGTCTGTTCATTCTTCGACCC....................................................................	21	1	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
...........................................................................................................ATCTGTTCATTCTTCGACCAAA..................................................................	22	1	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
............................................................................................................TCGGTTCATTCTTCGACCCA...................................................................	20	1	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
............................................................................................................TCTGTTCATTCTTCGACCCAAAGA...............................................................	24	1	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
............................................................................................................TCTGTTCATTCTTCGACCCCAA.................................................................	22	1	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
.............................................................CCGGGTTGGTGGTTGAAC....................................................................................................................	18	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0
............................................................................................................TCTGTTCATTCTTCCACCCA...................................................................	20	1	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
............................................................................................................TCTGTTCATTCTTCGACCCATTT................................................................	23	3	2	0.50	1	0	1	0	0	0	0	0	0	0	0	0
.......................................................................GGTTGAACTTTGATGCTAAGC.......................................................................................................	21	3	5	0.20	1	0	1	0	0	0	0	0	0	0	0	0
...................................................................................................................ATTCTTCAACCCAAGAAG..............................................................	18	2	7	0.14	1	0	0	0	0	0	0	0	0	0	1	0
............................................................................................................................................................ACAGATGTGTGAATGTA......................	17	2	20	0.05	1	0	0	0	0	0	0	0	0	0	0	1
......................................................................................................................................TTACGTGCAGAAGGAGCGA..........................................	19	3	20	0.05	1	0	1	0	0	0	0	0	0	0	0	0

CACGACCAGTGACAATTGAGTTTACATTCACACATTTGATGTTCGCTGCTTCTTCACGTGAACTTTTTGGGTCGAAGAATGAACAGATTTATCTTATTTAAATGCTTAAAATCGAAGTTCAACCACCAACCCGGAAAAGTATACGTTAAAGTCATATAATCTGAAAGAATATTTGAATTGGTTCGTATACCCTGT *************************************************....((((..((((((.(((((((.((.(((((.(((((..................)))))...))))).)).)))))))..))))))..))))...********************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	SRR553487 Ovary	SRR553488 Ovary
.............................................................................................................................................TACGTTAAAGTCATATAATCTGAAAGAA..........................	28	0	1	2.00	2	2	0
...............................................................................TGAACAGATTTATCTTATTTAAATGCT.........................................................................................	27	0	1	1.00	1	0	1
..................................................................................................................................................TAAAGTCATATAATCTGAAAGAATATT......................	27	0	1	1.00	1	0	1
.............................................................................................................................................TACGTTAAAGTCATATAATCTGAAAGA...........................	27	0	1	1.00	1	1	0
...................................................................TGGGTCGAAGAATGAACAGATTTATC......................................................................................................	26	0	1	1.00	1	0	1
...............................................................................................................................................CGTTAAAGTCATATAATCTGAAAGA...........................	25	0	1	1.00	1	1	0
...............................................................................................................................................................TCTGAAAGAATATTCGAATTGGTT............	24	1	1	1.00	1	0	0
...............................................................................................................TCGAAGTTCAACCACCAACCCGGAAAA.........................................................	27	0	1	1.00	1	0	1
...........................................CGCTGATTCTTGACATGAAC....................................................................................................................................	20	3	4	0.75	3	0	0
...............................................................................TGGACAGAATTATCTTTTTTA...............................................................................................	21	3	12	0.08	1	0	1
.......................................................ACGTGAACTTTTTGGGGA..........................................................................................................................	18	2	13	0.08	1	0	0

Species	Coordinate	ID	Alignment
droSim3	X:15011254-15011448 +	dsi_170	ACA-GGGTATACG--------AACCAATTCAAATATTCTTTC-AGATTATATGACT----------------------------------------------------------------------TT---AACGTATACTTTTCCGGGTTGGTGGTTGAACTTCGATTTTAA--GCATTTAAATAAG---------A-TAAATCTGTTCATTCTTCGACCCAAAAAGTTCAC-GTGAAGAAGCAGCGAACAT------------------------CAAATGTGTGA------------------AT---------------G------------------------------------------TA-AA---CTC-AATTGTCACTGGTCGTG
droSec2	scaffold_8:1727924-1728129 +	dse_159	ACA-GGGTATACGTTTAAACAAACCAATTCGAATATTCTTTC-AGATTATATGACT----------------------------------------------------------------------TC---AACGTATACTTTTCGGGGTTGGTGGTTGAACTTCGATTTTAG--GTATTTAAATAAG---------A-TAAATCTGTTCATTCTTCGACCCAAAAAGTTCAC-GTGAAGAAGCAGCGAGCAT------------------------CAAATGAGTGA------------------AT----C--------AGG------------------------------------------TA-AA---CTC-GATTGTCACTGGTCGTG
dm3	chrX:19445471-19445728 +	dme-mir-973	ACA-GGGTATACGTTTGAACAAACCAATTCGAATATTCTTTG-TGATTTTGCG-CT------------------------AAACATCTGCTTT-----------TAACATCAGAATATACTTCACTTTACCGTCGTCGACTTTTCGTGGTTGGTGGTTGAACTTCGATTTTAA--GTATTTAAATAAA---------ATGAAATCTGTTCATTCTCCGACACAAGAAGTTCAC-GCAAAGGAGCTGCAGCTGC------------------GAACATCAAATGCGTGA------------------AT----CCCAGGTCCAGG------------------------------------------TA-AG---CTC-AATTGTCACCGGTCATG
droEre2	scaffold_4690:9682208-9682469 +	der_78	ACA-GGGTACCAGATTCAACAAACCAAAGCGAGG-----------CC----TAACTAATTTATGTGTAATATTTTACACAG------TACTTTCCTTAATCTATATAAATCAGACTATAC--CATTTT---AACGTCGACTTTTCGTGGTTGGTGGCTGAACTTAGATTTTGA--GTATTCAAATAAA---------A-TAAATCTGTTCATTCCTCGACACAAGAAGTTGAC-GTGCAGGAGCGGCAAACAT------------------------GAATCGAGTGA------------------AT----C--------AGGAAAACA-------GA---------------------------AC-CA---CTA-ACTCAGCACTGGGCATG
droEug1	scf7180000409109:6796-6991 -		ACTTTTATAAATC----------------------A---------TT--AAAGATT----------------------------------------------------------------------CC---AACGACGACTTCTCGTGTTTGGTGACTGAACTTGGATTTTGA--ATATTTAAATAAA---------A-TAAATCTGTTCAATCCTCAACACGAGAAGTCGAC-GTAAATGACCAGCAAACAT------------------------AAATAAGGTGATAGTGCTTAA--------AA----CTAAAACA---------------------------------------TAATAATTA-AG---ATT-AAGAATAATTGGATATG
droBia1	scf7180000302069:1157414-1157584 -		ATT------------------------------------------------TGATG----------------------------------------------------------------------TT---AACGCCGACTTTAGGTGTTTGGTGGCTGAACTTGGATTTTTA--ACAGTTGAATGTA---------T-TAAATCTGTTCATTCCTCAACACAAAAAGTTGAC-GTTAAGGCCCAGCATAAAA-------------------------AACAGAGTGA------------------TG----A--------GAC------AAGGCAATACAAA----------------------T-TTAG------AATTTAGAAAAGGTCATC
droTak1	scf7180000415169:850687-850874 -		ACT-AAGCATAT---TGAGAA----------AATATTATTAA-AGTT----TAATT----------------------------------------------------------------------TT---AACGTCGACTTTTCGTGTTTGGGGGCTGAACTCTGATTTTTA--ATAATTGAATTAA---------G-TAAATCTGTTCATTCCTCGACACAAGAAGTTGGC-GTAAAGTAACAGCGCATAC------------------------AAATTGAGTGA------------------TAATA------------A-----------------------------------------T-TTGG------AACTTCGAATAGGTCATC
droEle1	scf7180000490751:1106866-1107019 +		-------------------------------------------------------------------------------------------------------------------------------T---GACATCGACTTTTTGTGTTTGGCGGATGAACTTAGATTTTTC--TTATTTGTA-AAA---------A-CAAATCTGTTCATTCCTCGACGCAAAAAGTTGATTGTTAAAAACCAGCACACTC------------------------AAATGGAGCAA------------------AAAAA-----------------AA-------TA---------------------------CG-GG---CTG-ACTCGGAAAAGGTCATT
droRho1	scf7180000761121:198679-198868 -		TAT-CTA--------------AATAAATTCAA-AATGCTTAATAATT--CAACATT----------------------------------------------------------------------AA---AACATCGACTTCTCGTGTTTGGCGTCTGAACTCAGATTCTTA--ACAGTTGTGTTAA---------ATAAAATCTGTTCATTCCTCGACACAAGAAGTTGAC-GTTAAG-ACCAGCATACTC------------------------AACTGAAGAGA------------------AAACA------------A------------------------------------------CG-AG---CTT-TCTCGAAATAGGTCATC
droFic1	scf7180000454073:1723908-1724095 +		ATCAGTTTAAACA----------------------ATTTGGA-AAAATACAAAACG----------------------------------------------------------------------GT---GACATTGACATCTCGTGTTTGGCGAATGAGCTCGGATTTTTGTAGTATTTGAATAAG---------A-TAAATCTGTTCATTCCTCGACACAAGAAGTTGAA-GTTGATAATCAACTAAAACAAAGTGCTAAGGATACGC------A---------------------------------------------------------------------------------------A-TTAA------AAACAAAAAAGGAGCATT
droKik1	scf7180000302517:1176220-1176378 +		A------------------------------------------------------------------------------------------------------------------------------------------CTTTTCGTGCTTGGCGATTGAAGGTGGATTTGTG--ATTTTTAAG-CAA---------T-TAAATCTGTTCAATCGTCAACACTAAAAGTTGAT-GTTATTTAGCTTCGAACCC------------------------CAAGGAT-----CCTGCCTAGAACGGTGAGTATAGC-----------------------------------------------------CG-GGACATCT-CCGCCAAGCTGGTCTTT
droAna3	scaffold_13417:2478569-2478743 -	dan_104	A------------------------------------------------------------------------------------------------------------------------------------------CTCCTCGTGTTTGGCGATTGAACCTGGATTTTTA--ATTTTCGAGTACG---------A-GAAATCTGTTCATTCCTCGACACTAGTAGTTGGC-GCGACA-CTCAGCGCACCA-------------------AACATGAACCGCG--G------------------CT----C-----------------AAATCAATCCAATCCGAATCCAATCGCGGTTGCAAT----C---CTG-GCTTGGGGTAGGTCATC
droWil2	scf2_1100000004909:7946469-7946631 +	dwi_138	TC---------------------------------------------------------------------------------------------------------------------------------GACATGGCCTTTTCGTGTTTGGTGATTGAACTTGGATTTTGT-TCTATATACATATATAAAAATATG-TAAATCTGTTCATTCCTCGACACTAGAAGTTCAT-GTCAATAAC-TTCATTTCC----TGC-----------ACACAAGAAAAGCG--A------------------AT----C---------------------------------------------------------A---TTCATTTTTCCAATGGCCATA
droVir3	scaffold_13042:918331-918433 -	dvi_50	A------------------------------------------------------------------------------------------------------------------------------------CATTCACTTTTCGTGTTCGATGTTTGAACTTGGATTTTTT--TTATAT-----AC---------A-CAAATCTGTTCATTCCTCGACACTAGAAGTTTGC-GTCAATAAGCTGCCAATAT----------------------------------------------------------------------------------------------------------------------------------------------

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droSim3

ACA-GGGTATACG--------AACCAATTCAAATATTCTTTC-AGATTATATGACT----------------------------------------------------------------------TT---AACGTATACTTTTCCGGGTTGGTGGTTGAACTTCGATTTTAA--GCATTTAAATAAG---------A-TAAATCTGTTCATTCTTCGACCCAAAAAGTTCAC-GTGAAGAAGCAGCGAACAT------------------------CAAATGTGTGA------------------AT---------------G------------------------------------------TA-AA---CTC-AATTGTCACTGGTCGTG	Size	Hit Count	Total Norm	Total	V044 Embryo	M023 Head	M024 Male-body	M025 Embryo	O001 Testis	O002 Head	M053 Female-body	SRR553485 Ovary	SRR553486 Ovary	SRR553487 Ovary	SRR553488 Ovary	SRR618934 Ovary
...........................................................................................................................................................................................................TCTGTTCATTCTTCGACCCAA...........................................................................................................................................................................	21	1	898.00	898	0	7	200	0	678	8	0	0	2	1	2	0
...........................................................................................................................................................................................................TCTGTTCATTCTTCGACCCAAA..........................................................................................................................................................................	22	1	467.00	467	0	3	272	1	182	1	0	4	0	1	3	0
..........................................................................................................................................................................................................ATCTGTTCATTCTTCGACCCA............................................................................................................................................................................	21	1	302.00	302	0	0	15	0	281	4	1	0	0	0	0	1
..........................................................................................................................................................................................................ATCTGTTCATTCTTCGACCCAA...........................................................................................................................................................................	22	1	273.00	273	0	0	7	0	258	6	0	0	0	1	1	0
...........................................................................................................................................................................................................TCTGTTCATTCTTCGACCC.............................................................................................................................................................................	19	1	223.00	223	0	1	9	0	208	5	0	0	0	0	0	0
...........................................................................................................................................................................................................TCTGTTCATTCTTCGACCCA............................................................................................................................................................................	20	1	216.00	216	0	17	66	0	132	1	0	0	0	0	0	0
..........................................................................................................................................................................................................ATCTGTTCATTCTTCGACCC.............................................................................................................................................................................	20	1	108.00	108	0	0	3	0	102	3	0	0	0	0	0	0
..........................................................................................................................................................................................................ATCTGTTCATTCTTCGACCCAAA..........................................................................................................................................................................	23	1	95.00	95	0	0	5	0	88	1	0	1	0	0	0	0
.........................................................................................................................................................................................................AATCTGTTCATTCTTCGACCCA............................................................................................................................................................................	22	1	60.00	60	0	0	12	0	45	0	0	1	1	1	0	0
...........................................................................................................................................................................................................TCTGTTCATTCTTCGACC..............................................................................................................................................................................	18	1	44.00	44	0	3	6	0	35	0	0	0	0	0	0	0
..........................................................................................................................................................................................................ATCTGTTCATTCTTCGACC..............................................................................................................................................................................	19	1	33.00	33	0	1	2	0	30	0	0	0	0	0	0	0
...........................................................................................................................................................................................................TCTGTTCATTCTTCGACCCAAAA.........................................................................................................................................................................	23	1	26.00	26	0	0	12	0	14	0	0	0	0	0	0	0
.........................................................................................................................................................................................................AATCTGTTCATTCTTCGACCC.............................................................................................................................................................................	21	1	24.00	24	0	1	11	0	12	0	0	0	0	0	0	0
..........................................................................................................................................................................................................ATCTGTTCATTCTTCGAC...............................................................................................................................................................................	18	1	9.00	9	0	0	0	0	9	0	0	0	0	0	0	0
............................................................................................................................................................................................................CTGTTCATTCTTCGACCCAA...........................................................................................................................................................................	20	1	7.00	7	0	0	1	0	6	0	0	0	0	0	0	0
.........................................................................................................................................................................................................AATCTGTTCATTCTTCGACC..............................................................................................................................................................................	20	1	7.00	7	0	0	1	0	6	0	0	0	0	0	0	0
.........................................................................................................................................................................................................AATCTGTTCATTCTTCGACCCAA...........................................................................................................................................................................	23	1	6.00	6	0	0	2	0	4	0	0	0	0	0	0	0
............................................................................................................................................................................................................CTGTTCATTCTTCGACCCA............................................................................................................................................................................	19	1	6.00	6	0	0	0	0	6	0	0	0	0	0	0	0
................................................................................................................................................CCGGGTTGGTGGTTGAACTTCGATTT.................................................................................................................................................................................................................................	26	1	6.00	6	0	0	0	0	6	0	0	0	0	0	0	0
................................................................................................................................................CCGGGTTGGTGGTTGAACTTCGAT...................................................................................................................................................................................................................................	24	1	6.00	6	0	0	0	0	6	0	0	0	0	0	0	0
.........................................................................................................................................................................................................AATCTGTTCATTCTTCGAC...............................................................................................................................................................................	19	1	4.00	4	0	0	2	0	2	0	0	0	0	0	0	0
..........................................................................................................................................................................................................ATCTGTTCATTCTTCGACCCAAAA.........................................................................................................................................................................	24	1	4.00	4	0	0	0	0	4	0	0	0	0	0	0	0
................................................................................................................................................CCGGGTTGGTGGTTGAACTTC......................................................................................................................................................................................................................................	21	1	3.00	3	0	0	0	0	3	0	0	0	0	0	0	0
............................................................................................................................................................................................................CTGTTCATTCTTCGACCCAAA..........................................................................................................................................................................	21	1	2.00	2	0	0	0	0	2	0	0	0	0	0	0	0
................................................................................................................................................CCGGGTTGGTGGTTGAACTTCG.....................................................................................................................................................................................................................................	22	1	2.00	2	0	0	0	0	1	0	0	1	0	0	0	0
.........................................................................................................................................................................................................AATCTGTTCATTCTTCGA................................................................................................................................................................................	18	1	2.00	2	0	0	0	0	2	0	0	0	0	0	0	0
............................................................................................................................................................................................................CTGTTCATTCTTCGACCC.............................................................................................................................................................................	18	1	2.00	2	0	0	0	0	2	0	0	0	0	0	0	0
..........................................................................................................................................................................TAA--GCATTTAAATAAG---------A-TAA.................................................................................................................................................................................................	20	1	2.00	2	0	0	0	0	2	0	0	0	0	0	0	0
..............................................................................................................................................................................................................GTTCATTCTTCGACCCAA...........................................................................................................................................................................	18	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
................................................................................................................................................CCGGGTTGGTGGTTGAACTTCGATT..................................................................................................................................................................................................................................	25	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
...............................................................................................................................................TCCGGGTTGGTGGTTGAACTTCGAT...................................................................................................................................................................................................................................	25	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
...............................................................................................................................................................................................................TTCATTCTTCGACCCAAA..........................................................................................................................................................................	18	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
..................................................................................................................................................GGGTTGGTGGTTGAACTTCGATT..................................................................................................................................................................................................................................	23	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
................................................................................................................................................CCGGGTTGGTGGTTGAAC.........................................................................................................................................................................................................................................	18	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0

droSec2

ACA-GGGTATACGTTTAAACAAACCAATTCGAATATTCTTTC-AGATTATATGACT----------------------------------------------------------------------TC---AACGTATACTTTTCGGGGTTGGTGGTTGAACTTCGATTTTAG--GTATTTAAATAAG---------A-TAAATCTGTTCATTCTTCGACCCAAAAAGTTCAC-GTGAAGAAGCAGCGAGCAT------------------------CAAATGAGTGA------------------AT----C--------AGG------------------------------------------TA-AA---CTC-GATTGTCACTGGTCGTG	Size	Hit Count	Total Norm	Total	V113 Male-body	V114 Embryo	V115 Head	M054 Female-body
..........................................................................................................................................................................................................ATCTGTTCATTCTTCGACCCA............................................................................................................................................................................	21	1	6.00	6	1	1	0	4
..........................................................................................................................................................................................................ATCTGTTCATTCTTCGACCCAA...........................................................................................................................................................................	22	1	6.00	6	3	1	2	0
...........................................................................................................................................................................................................TCTGTTCATTCTTCGACCCAA...........................................................................................................................................................................	21	1	3.00	3	2	1	0	0
...........................................................................................................................................................................................................TCTGTTCATTCTTCGACCC.............................................................................................................................................................................	19	1	2.00	2	0	0	0	2
...........................................................................................................................................................................................................TCTGTTCATTCTTCGACCCAAA..........................................................................................................................................................................	22	1	2.00	2	0	1	0	1
..........................................................................................................................................................................................................ATCTGTTCATTCTTCGACCCAAA..........................................................................................................................................................................	23	1	2.00	2	1	0	0	1
.................................................................................................................................................GGGGTTGGTGGTTGAACTTCG.....................................................................................................................................................................................................................................	21	1	1.00	1	1	0	0	0
...............................................................................................................................................................................................................TTCATTCTTCGACCCAAAAAGT......................................................................................................................................................................	22	1	1.00	1	1	0	0	0
.................................................................................................................................................GGGGTTGGTGGTTGAACTTCGA....................................................................................................................................................................................................................................	22	1	1.00	1	1	0	0	0
................................................................................................................................................CGGGGTTGGTGGTTGAACTTCGATT..................................................................................................................................................................................................................................	25	1	1.00	1	0	1	0	0

droEre2

ACA-GGGTACCAGATTCAACAAACCAAAGCGAGG-----------CC----TAACTAATTTATGTGTAATATTTTACACAG------TACTTTCCTTAATCTATATAAATCAGACTATAC--CATTTT---AACGTCGACTTTTCGTGGTTGGTGGCTGAACTTAGATTTTGA--GTATTCAAATAAA---------A-TAAATCTGTTCATTCCTCGACACAAGAAGTTGAC-GTGCAGGAGCGGCAAACAT------------------------GAATCGAGTGA------------------AT----C--------AGGAAAACA-------GA---------------------------AC-CA---CTA-ACTCAGCACTGGGCATG	Size	Hit Count	Total Norm	Total	V040 Embryo	V060 Head	V107 Male-body	V108 Head	M055 Female-body	M057 Embryo
...........................................................................................................................................................................................................TCTGTTCATTCCTCGACACAAG..........................................................................................................................................................................	22	1	34.00	34	0	1	13	4	16	0
...........................................................................................................................................................................................................TCTGTTCATTCCTCGACACAA...........................................................................................................................................................................	21	1	13.00	13	0	0	6	2	5	0
...........................................................................................................................................................................................................TCTGTTCATTCCTCGACACA............................................................................................................................................................................	20	1	5.00	5	0	1	3	1	0	0
..........................................................................................................................................................................................................ATCTGTTCATTCCTCGACACAAG..........................................................................................................................................................................	23	1	3.00	3	0	0	0	0	2	1
................................................................................................................................................CGTGGTTGGTGGCTGAACTTAG.....................................................................................................................................................................................................................................	22	1	2.00	2	0	0	2	0	0	0
................................................................................................................................................CGTGGTTGGTGGCTGAACTTAGA....................................................................................................................................................................................................................................	23	1	1.00	1	0	0	0	1	0	0
................................................................................................................................................CGTGGTTGGTGGCTGAACTTAGATT..................................................................................................................................................................................................................................	25	1	1.00	1	0	0	1	0	0	0
................................................................................................................................................CGTGGTTGGTGGCTGAACTTAGAT...................................................................................................................................................................................................................................	24	1	1.00	1	0	0	0	0	1	0

droEug1

ACTTTTATAAATC----------------------A---------TT--AAAGATT----------------------------------------------------------------------CC---AACGACGACTTCTCGTGTTTGGTGACTGAACTTGGATTTTGA--ATATTTAAATAAA---------A-TAAATCTGTTCAATCCTCAACACGAGAAGTCGAC-GTAAATGACCAGCAAACAT------------------------AAATAAGGTGATAGTGCTTAA--------AA----CTAAAACA---------------------------------------TAATAATTA-AG---ATT-AAGAATAATTGGATATG	Size	Hit Count	Total Norm	Total
No Reads

droBia1

ATT------------------------------------------------TGATG----------------------------------------------------------------------TT---AACGCCGACTTTAGGTGTTTGGTGGCTGAACTTGGATTTTTA--ACAGTTGAATGTA---------T-TAAATCTGTTCATTCCTCAACACAAAAAGTTGAC-GTTAAGGCCCAGCATAAAA-------------------------AACAGAGTGA------------------TG----A--------GAC------AAGGCAATACAAA----------------------T-TTAG------AATTTAGAAAAGGTCATC	Size	Hit Count	Total Norm	Total
No Reads

droTak1

ACT-AAGCATAT---TGAGAA----------AATATTATTAA-AGTT----TAATT----------------------------------------------------------------------TT---AACGTCGACTTTTCGTGTTTGGGGGCTGAACTCTGATTTTTA--ATAATTGAATTAA---------G-TAAATCTGTTCATTCCTCGACACAAGAAGTTGGC-GTAAAGTAACAGCGCATAC------------------------AAATTGAGTGA------------------TAATA------------A-----------------------------------------T-TTGG------AACTTCGAATAGGTCATC	Size	Hit Count	Total Norm	Total
No Reads

droEle1

-------------------------------------------------------------------------------------------------------------------------------T---GACATCGACTTTTTGTGTTTGGCGGATGAACTTAGATTTTTC--TTATTTGTA-AAA---------A-CAAATCTGTTCATTCCTCGACGCAAAAAGTTGATTGTTAAAAACCAGCACACTC------------------------AAATGGAGCAA------------------AAAAA-----------------AA-------TA---------------------------CG-GG---CTG-ACTCGGAAAAGGTCATT	Size	Hit Count	Total Norm	Total
No Reads

droRho1

TAT-CTA--------------AATAAATTCAA-AATGCTTAATAATT--CAACATT----------------------------------------------------------------------AA---AACATCGACTTCTCGTGTTTGGCGTCTGAACTCAGATTCTTA--ACAGTTGTGTTAA---------ATAAAATCTGTTCATTCCTCGACACAAGAAGTTGAC-GTTAAG-ACCAGCATACTC------------------------AACTGAAGAGA------------------AAACA------------A------------------------------------------CG-AG---CTT-TCTCGAAATAGGTCATC	Size	Hit Count	Total Norm	Total
No Reads

droFic1

ATCAGTTTAAACA----------------------ATTTGGA-AAAATACAAAACG----------------------------------------------------------------------GT---GACATTGACATCTCGTGTTTGGCGAATGAGCTCGGATTTTTGTAGTATTTGAATAAG---------A-TAAATCTGTTCATTCCTCGACACAAGAAGTTGAA-GTTGATAATCAACTAAAACAAAGTGCTAAGGATACGC------A---------------------------------------------------------------------------------------A-TTAA------AAACAAAAAAGGAGCATT	Size	Hit Count	Total Norm	Total
No Reads

droKik1

A------------------------------------------------------------------------------------------------------------------------------------------CTTTTCGTGCTTGGCGATTGAAGGTGGATTTGTG--ATTTTTAAG-CAA---------T-TAAATCTGTTCAATCGTCAACACTAAAAGTTGAT-GTTATTTAGCTTCGAACCC------------------------CAAGGAT-----CCTGCCTAGAACGGTGAGTATAGC-----------------------------------------------------CG-GGACATCT-CCGCCAAGCTGGTCTTT	Size	Hit Count	Total Norm	Total
No Reads

droAna3

A------------------------------------------------------------------------------------------------------------------------------------------CTCCTCGTGTTTGGCGATTGAACCTGGATTTTTA--ATTTTCGAGTACG---------A-GAAATCTGTTCATTCCTCGACACTAGTAGTTGGC-GCGACA-CTCAGCGCACCA-------------------AACATGAACCGCG--G------------------CT----C-----------------AAATCAATCCAATCCGAATCCAATCGCGGTTGCAAT----C---CTG-GCTTGGGGTAGGTCATC	Size	Hit Count	Total Norm	Total	V105 Male-body	V106 Head	V055 Head	V039 Embryo	M044 Female-body	M058 Embryo
...........................................................................................................................................................................................................TCTGTTCATTCCTCGACACTAG..........................................................................................................................................................................	22	1	70.00	70	9	2	2	0	57	0
...........................................................................................................................................................................................................TCTGTTCATTCCTCGACACTA...........................................................................................................................................................................	21	1	8.00	8	4	0	0	0	4	0
............................................................................................................................................................................................................CTGTTCATTCCTCGACACTAG..........................................................................................................................................................................	21	1	8.00	8	0	0	1	0	7	0
...........................................................................................................................................................................................................TCTGTTCATTCCTCGACACTAGT.........................................................................................................................................................................	23	1	6.00	6	4	2	0	0	0	0
................................................................................................................................................CGTGTTTGGCGATTGAACCTGG.....................................................................................................................................................................................................................................	22	1	5.00	5	5	0	0	0	0	0
................................................................................................................................................CGTGTTTGGCGATTGAACCTGGATT..................................................................................................................................................................................................................................	25	1	2.00	2	0	0	1	0	1	0
................................................................................................................................................CGTGTTTGGCGATTGAACCTGGAT...................................................................................................................................................................................................................................	24	1	2.00	2	2	0	0	0	0	0
...........................................................................................................................................................................................................TCTGTTCATTCCTCGACACT............................................................................................................................................................................	20	1	2.00	2	0	1	0	0	1	0
..................................................................................................................................................TGTTTGGCGATTGAACCTGGATT..................................................................................................................................................................................................................................	23	1	1.00	1	1	0	0	0	0	0
................................................................................................................................................CGTGTTTGGCGATTGAACCTG......................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	1	0
.............................................................................................................................................................................................................TGTTCATTCCTCGACACTAG..........................................................................................................................................................................	20	1	1.00	1	0	0	0	0	1	0
............................................................................................................................................................................................................CTGTTCATTCCTCGACACTAGT.........................................................................................................................................................................	22	1	1.00	1	1	0	0	0	0	0
..............................................................................................................................................................................................................GTTCATTCCTCGACACTAG..........................................................................................................................................................................	19	1	1.00	1	0	0	0	0	1	0
..................................................................................................................................................TGTTTGGCGATTGAACCTGGA....................................................................................................................................................................................................................................	21	1	1.00	1	1	0	0	0	0	0

droWil2

TC---------------------------------------------------------------------------------------------------------------------------------GACATGGCCTTTTCGTGTTTGGTGATTGAACTTGGATTTTGT-TCTATATACATATATAAAAATATG-TAAATCTGTTCATTCCTCGACACTAGAAGTTCAT-GTCAATAAC-TTCATTTCC----TGC-----------ACACAAGAAAAGCG--A------------------AT----C---------------------------------------------------------A---TTCATTTTTCCAATGGCCATA	Size	Hit Count	Total Norm	Total	V117 Male-body	V118 Embryo	V119 Head	M020 Head	M045 Female-body
............................................................................................................................................TTTTCGTGTTTGGTGATTGAACT........................................................................................................................................................................................................................................	23	1	64.00	64	30	0	1	33	0
............................................................................................................................................TTTTCGTGTTTGGTGATTGAAC.........................................................................................................................................................................................................................................	22	1	30.00	30	29	0	0	1	0
...........................................................................................................................................................................................................TCTGTTCATTCCTCGACACTAG..........................................................................................................................................................................	22	1	23.00	23	0	0	0	23	0
...............................................................................................................................................................................................................TTCATTCCTCGACACTAGAAGT......................................................................................................................................................................	22	1	20.00	20	20	0	0	0	0
...........................................................................................................................................................................................................TCTGTTCATTCCTCGACACTAGA.........................................................................................................................................................................	23	1	13.00	13	0	0	0	13	0
............................................................................................................................................TTTTCGTGTTTGGTGATTGAA..........................................................................................................................................................................................................................................	21	1	9.00	9	6	0	0	3	0
...........................................................................................................................................................................................................TCTGTTCATTCCTCGACACT............................................................................................................................................................................	20	1	5.00	5	0	0	0	5	0
............................................................................................................................................TTTTCGTGTTTGGTGATTGA...........................................................................................................................................................................................................................................	20	1	5.00	5	5	0	0	0	0
...........................................................................................................................................................................................................TCTGTTCATTCCTCGACACTA...........................................................................................................................................................................	21	1	2.00	2	1	0	0	1	0
.............................................................................................................................................TTTCGTGTTTGGTGATTGAACT........................................................................................................................................................................................................................................	22	1	2.00	2	1	0	0	1	0
............................................................................................................................................TTTTCGTGTTTGGTGATTGAACTT.......................................................................................................................................................................................................................................	24	1	2.00	2	0	0	0	2	0
................................................................................................................................................CGTGTTTGGTGATTGAACTTGGATT..................................................................................................................................................................................................................................	25	1	2.00	2	0	1	1	0	0
...............................................................................................................................................................................................................TTCATTCCTCGACACTAGAAG.......................................................................................................................................................................	21	1	1.00	1	0	0	0	1	0
...........................................................................................................................................................................................................TCTGTTCATTCCTCGACAC.............................................................................................................................................................................	19	1	1.00	1	0	0	0	1	0
...............................................................................................................................................................................................................TTCATTCCTCGACACTAGAAGTT.....................................................................................................................................................................	23	1	1.00	1	1	0	0	0	0

droVir3

A------------------------------------------------------------------------------------------------------------------------------------CATTCACTTTTCGTGTTCGATGTTTGAACTTGGATTTTTT--TTATAT-----AC---------A-CAAATCTGTTCATTCCTCGACACTAGAAGTTTGC-GTCAATAAGCTGCCAATAT----------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	V116 Male-body	V053 Head	V047 Embryo	M027 Male-body	M028 Head	M047 Female-body	M061 Embryo	SRR060654 Ovary	SRR060655 Testis	SRR060656 Ovary	SRR060657 Testis	SRR060658 Ovary	SRR060659 Testis	SRR060660 Ovary	SRR060661 Embryo	SRR060662 Embryo	SRR060663 Embryo	SRR060664 Male-body	SRR060665 Female-body	SRR060666 Male-body	SRR060667 Female-body	SRR060668 Male-body	SRR060669 Female-body	SRR060670 Testis	SRR060671 Male-body	SRR060672 Female-body	SRR060673 Ovary	SRR060674 Ovary	SRR060675 Ovary	SRR060676 Ovary	SRR060677 Ovary	SRR060678 Testis	SRR060679 Testis	SRR060680 Testis	SRR060681 Testis	SRR060682 Embryo	SRR060683 Testis	SRR060684 Embryo	SRR060685 Embryo	SRR060686 Embryo	SRR060687 Embryo	SRR060688 Ovary	SRR060689 Testis
...........................................................................................................................................................................................................TCTGTTCATTCCTCGACACTAG..........................................................................................................................................................................	22	1	17810.00	17810	12	1	1	276	3	237	5	0	72	0	1	0	2	0	0	0	0	1	0	0	0	1	0	174	0	0	0	13	6	8	7	6206	3637	1525	5156	3	148	3	1	1	3	2	305
..........................................................................................................................................................................................................ATCTGTTCATTCCTCGACACTAG..........................................................................................................................................................................	23	1	9286.00	9286	7	0	0	13	0	53	6	0	37	0	0	0	0	0	0	0	0	1	0	0	1	0	0	73	1	0	0	5	0	3	4	3430	1814	838	2833	0	63	0	2	1	0	0	101
..........................................................................................................................................................................................................ATCTGTTCATTCCTCGACACT............................................................................................................................................................................	21	1	5832.00	5832	9	0	0	123	2	24	5	0	54	0	5	0	1	0	0	0	0	0	0	0	0	1	0	70	2	1	4	5	1	2	5	1716	1193	596	1722	1	99	1	0	0	0	0	190
...........................................................................................................................................................................................................TCTGTTCATTCCTCGACACTA...........................................................................................................................................................................	21	1	2692.00	2692	2	0	0	24	1	36	1	1	21	0	1	0	0	0	0	0	0	3	0	0	0	0	0	25	0	0	1	2	1	1	0	849	540	299	799	0	18	0	0	0	0	0	67
...........................................................................................................................................................................................................TCTGTTCATTCCTCGACACT............................................................................................................................................................................	20	1	2532.00	2532	1	0	0	247	9	32	1	0	28	0	1	0	1	0	0	0	0	1	0	1	0	1	0	59	1	0	2	1	2	0	1	538	512	251	694	1	54	0	1	0	0	0	92
..........................................................................................................................................................................................................ATCTGTTCATTCCTCGACACTA...........................................................................................................................................................................	22	1	1915.00	1915	1	0	0	1	0	15	1	0	3	0	0	0	0	0	0	0	0	0	0	0	0	2	0	18	0	0	0	0	0	0	1	684	396	191	551	0	20	0	0	2	1	0	28
..........................................................................................................................................................................................................ATCTGTTCATTCCTCGACAC.............................................................................................................................................................................	20	1	391.00	391	0	0	0	2	0	9	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	3	0	0	0	0	0	0	0	127	86	41	113	0	3	0	0	0	0	0	5
............................................................................................................................................................................................................CTGTTCATTCCTCGACACTAG..........................................................................................................................................................................	21	1	284.00	284	0	0	0	0	0	27	0	0	4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	84	66	14	85	0	2	0	0	0	0	0	1
..........................................................................................................................................................................................................ATCTGTTCATTCCTCGACA..............................................................................................................................................................................	19	1	204.00	204	0	0	0	2	0	7	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	3	0	0	0	2	7	0	1	57	58	17	43	0	5	0	0	0	0	1	0
................................................................................................................................................CGTGTTCGATGTTTGAACTTGGATTT.................................................................................................................................................................................................................................	26	1	137.00	137	1	0	0	0	0	1	0	0	7	0	2	0	0	0	0	0	0	0	0	0	0	0	0	4	0	0	0	0	0	0	0	47	22	12	35	0	3	0	0	0	0	0	3
...........................................................................................................................................................................................................TCTGTTCATTCCTCGACACTAGA.........................................................................................................................................................................	23	1	128.00	128	0	0	0	52	1	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	23	16	5	26	0	0	0	0	1	0	0	0
................................................................................................................................................CGTGTTCGATGTTTGAACTTGGATT..................................................................................................................................................................................................................................	25	1	108.00	108	0	0	0	0	0	0	0	0	15	1	5	0	3	0	0	0	0	0	0	0	0	0	0	11	0	0	0	0	0	0	0	24	9	8	15	0	8	0	0	0	0	1	8
................................................................................................................................................CGTGTTCGATGTTTGAACTTGG.....................................................................................................................................................................................................................................	22	1	89.00	89	0	0	0	0	0	0	0	2	3	0	3	0	1	0	0	0	0	0	0	0	0	0	0	4	0	0	0	0	0	0	0	33	15	2	17	0	3	0	0	0	0	0	6
...........................................................................................................................................................................................................TCTGTTCATTCCTCGACAC.............................................................................................................................................................................	19	1	73.00	73	0	0	0	7	0	5	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	22	14	7	13	0	3	0	0	0	0	0	2
.............................................................................................................................................................................................................TGTTCATTCCTCGACACTAG..........................................................................................................................................................................	20	1	69.00	69	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	22	12	8	26	0	1	0	0	0	0	0	0
................................................................................................................................................CGTGTTCGATGTTTGAACTTGGA....................................................................................................................................................................................................................................	23	1	52.00	52	0	0	0	0	0	0	0	0	5	0	1	0	1	0	0	0	0	0	0	0	0	0	0	5	0	0	0	0	1	0	0	9	10	4	8	0	3	0	0	0	0	0	5
................................................................................................................................................CGTGTTCGATGTTTGAACTTGGAT...................................................................................................................................................................................................................................	24	1	45.00	45	0	0	0	0	0	0	0	0	2	0	0	0	2	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	1	0	7	9	5	9	0	3	0	0	0	0	0	5
...........................................................................................................................................................................................................TCTGTTCATTCCTCGACA..............................................................................................................................................................................	18	1	41.00	41	0	0	0	4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	5	12	6	9	0	2	0	0	0	0	0	2
..........................................................................................................................................................................................................ATCTGTTCATTCCTCGACACTAGA.........................................................................................................................................................................	24	1	36.00	36	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	12	10	4	10	0	0	0	0	0	0	0	0
............................................................................................................................................................................................................CTGTTCATTCCTCGACACTA...........................................................................................................................................................................	20	1	32.00	32	0	0	0	0	0	6	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	9	7	2	8	0	0	0	0	0	0	0	0
............................................................................................................................................................................................................CTGTTCATTCCTCGACACT............................................................................................................................................................................	19	1	30.00	30	0	0	0	1	0	5	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	9	4	3	8	0	0	0	0	0	0	0	0
................................................................................................................................................CGTGTTCGATGTTTGAACTTG......................................................................................................................................................................................................................................	21	1	16.00	16	0	0	0	0	0	0	0	0	1	0	2	0	2	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	3	4	0	2	0	0	0	0	0	0	0	0
.............................................................................................................................................................................................................TGTTCATTCCTCGACACT............................................................................................................................................................................	18	1	16.00	16	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	6	6	2	1	0	0	0	0	0	0	0	1
..............................................................................................................................................................................................................GTTCATTCCTCGACACTAG..........................................................................................................................................................................	19	1	14.00	14	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	6	2	1	3	0	0	0	0	0	0	0	0
.............................................................................................................................................................................................................TGTTCATTCCTCGACACTA...........................................................................................................................................................................	19	1	12.00	12	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	3	1	3	3	0	1	0	0	0	0	0	0
...............................................................................................................................................................................................................TTCATTCCTCGACACTAG..........................................................................................................................................................................	18	1	10.00	10	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	1	1	1	0	2	0	0	0	0	0	1
..........................................................................................................................................................................................................ATCTGTTCATTCCTCGAC...............................................................................................................................................................................	18	1	7.00	7	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	4	0	0	0	0	0	0	0	1
................................................................................................................................................CGTGTTCGATGTTTGAACT........................................................................................................................................................................................................................................	19	1	4.00	4	0	0	0	1	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	1
................................................................................................................................................CGTGTTCGATGTTTGAACTT.......................................................................................................................................................................................................................................	20	1	3.00	3	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	0	0	0	0	0	0	0	0	0	0
................................................................................................................................................CGTGTTCGATGTTTGAAC.........................................................................................................................................................................................................................................	18	1	2.00	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	0	0	0	0	0	0	0	0
............................................................................................................................................................................................................CTGTTCATTCCTCGACACTAGA.........................................................................................................................................................................	22	1	2.00	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	1	0	0	0	0	0	0	0	0
.................................................................................................................................................GTGTTCGATGTTTGAACTTGG.....................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1

dsi_170

X:15011304-15011398 +

Known Ortholog

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-25.2	-25.0	-24.8