ID:

dsi_169

 Coordinate:

X:15010940-15011042 +

 Confidence:

Known Ortholog

 Type:

Unknown

 [View on UCSC Genome Browser {Cornell Mirror}]
Legend: mature star mismatch in alignment mismatch in read

Predicted structure

-15.9

Flybase annnotation

intergenic

No Repeatable elements found

Sense Strand Reads

AACAACAATTCAAATGCAAGCAAAAAAAATAAAAAATAAAAAAGCTGAAGAGAATGATAGTGAATTGCTAAATATTTTCTTTGTATAAATGACTTTTAACATTTGTACAATATTAATATTTAAACATTTCTCGAATCAAAGTATGCCACATCAGCTTTGCAAGCCAAACGTGCGATGGACTAGTGACGAAAGACACTAAAAGT
*****************************************************........(((.((.((((((((....(((((((((((........)))))))))))....))))))))..)).))).....******************************************************************** 		Read size # Mismatch Hit Count Total Norm Total O001
Testis
 M024
male body
 M023
head
 O002
Head
 SRR553488
Ovary		 SRR618934
Ovary
....................................................................TAAATATTTTCTTTGTATAA................................................................................................................... 20 0 1 26.00 26 25 0 1 0 0 0
....................................................................TAAATATTTTCTTTGTATA.................................................................................................................... 19 0 1 6.00 6 6 0 0 0 0 0
....................................................................TAAATATTTTCTTTGTATAAA.................................................................................................................. 21 0 1 4.00 4 3 1 0 0 0 0
....................................................................TAAATATTTTCTTTGTATAAATG................................................................................................................ 23 0 1 3.00 3 2 1 0 0 0 0
......................................................................................................TTGTACAATATTAATATT................................................................................... 18 0 1 3.00 3 3 0 0 0 0 0
....................................................................TAAATATTTTCTTTGTAT..................................................................................................................... 18 0 1 3.00 3 3 0 0 0 0 0
.....................................................................................................TTTGTACAATATTAATATTTAAAC.............................................................................. 24 0 1 2.00 2 1 1 0 0 0 0
....................................................................TAAATATTTTCTTTGTATAAAT................................................................................................................. 22 0 1 2.00 2 1 0 0 0 1 0
......................................................................................................TTGTACAATATTAATATTTA................................................................................. 20 0 1 2.00 2 2 0 0 0 0 0
......................................................................................................................................................................AACGTGCGATGGACTAGTGACGAAAGA.......... 27 0 1 1.00 1 0 0 0 0 0 1
.......................................................................................................TGTACAATATTAATATTTAA................................................................................ 20 0 1 1.00 1 1 0 0 0 0 0
.....................................................................................................TTTGTACAATATTAATAT.................................................................................... 18 0 2 1.00 2 2 0 0 0 0 0
.......................................................................................................TGTACAATATTAATATTTA................................................................................. 19 0 1 1.00 1 0 0 0 1 0 0
.....................................................................................................TTTGTACAATATTAATATTT.................................................................................. 20 0 1 1.00 1 0 1 0 0 0 0
.....................................................................................................TTTGTACAATATTAATATTTA................................................................................. 21 0 1 1.00 1 1 0 0 0 0 0
....................................................................TAAATATTTTCTTTGTATAAAA................................................................................................................. 22 1 1 1.00 1 0 0 0 0 0 0
.....................................................................AAATATTTTCTTTGTATAA................................................................................................................... 19 0 1 1.00 1 1 0 0 0 0 0
..............................................................................ATTTGTATAAATGACTT............................................................................................................ 17 1 6 0.17 1 0 0 0 0 0 1
.........................................................................ATTTTCTTTGTACGTATGACT............................................................................................................. 21 3 6 0.17 1 0 1 0 0 0 0
...................................ATAAAAAAGGTGAAGA........................................................................................................................................................ 16 1 18 0.06 1 0 0 0 0 1 0
......................................................................................................................................ATCAAAGTATGTCAGAT.................................................... 17 2 20 0.05 1 0 0 0 0 0 0
.....................................................................................................................................................................................AGTGACGAATGACACCA..... 17 2 20 0.05 1 0 0 0 0 0 1

Anti-sense strand reads

ACTTTTAGTGTCTTTCGTCACTAGTCCATCGCACGTTTGGCTTGCAAAGCTGATGTGGCATACTTTGATTCGAGAAATGTTTAAATATTAATATTGTACAAATGTTAAAAGTCATTTATACAAAGAAAATATTTAGCAATTCACTATCATTCTCTTCAGCTTTTTTATTTTTTATTTTTTTTGCTTGCATTTGAATTGTTGTT
********************************************************************.....(((.((..((((((((....(((((((((((........)))))))))))....)))))))).)).)))........***************************************************** 		Read size # Mismatch Hit Count Total Norm Total SRR553487
Ovary
...............................................................TTTGATTCGAGAAATGTTTAAATATTAA................................................................................................................ 28 0 1 1.00 1 1
.............................................................................................TTGTACAAGTGTTAAAA............................................................................................. 17 1 5 0.20 1 0
............................................................................................................................................................................TATTTTTTTTAATTGCAGTTGAA........ 23 3 7 0.14 1 0
.............................................................................................................................................................AGCTGTATTATTTTTTATTT.......................... 20 2 16 0.06 1 0

Re-alignment of all predicted orthologs

Species Coordinate ID Alignment
droSim3 X:15010890-15011092 + dsi_169 AACAACA--------------ATTCAAATGCAAGCAA----AAAAAATAAAAAATAAAAAAGCTGAAGAGAATGATAGTGA-ATTGCTAAATATTTTCTTTGTATAAATGACTTTTAACATTTGTACAATATTAATATTTAAACATTTCTCGAATCAAAGTATGCCACATCAGCTTTGCAAGCCAAACGTGCGATGGACT----AGTGACGAAAGACACTAAAAGT
droSec2 scaffold_8:1727558-1727761 + AACAACA--------------ATTCAAATGCAAGCAATCTTAAAAA---AAAAATAAAAAAGCTGAAAAGAATGATAGTGA-ATTGCTAAATATTTTCTTTGTATAAATAACTTTTAATATTTGTACAATATTAATATTTAAACATTTCTCGAATCAAAGTATGCGACATCAGCTTTGCAAGCCAAACGTGCGATGGACT----AGTGACGAAAGACACTAAAAGT
dm3 chrX:19445123-19445329 + dme-mir-972 AAAAATACAGCAAATAGGGAGATTCAAACCCAT---ATTTGAAAAA---AAAATGCAAATAGCTAAAGAGAATGATAGGGAAATTGCTAAATATTTTTTTTGTATAAATAACTTTTAACTTTTGTACAATACGAATATTTAGGCATTTCTCAAATCAAA-------------GATTTGCAAGCCAAACGTGCAATGAACTACATAGTTACGAAAGACACTAAAAGT

Re-alignment of all predicted orthologs with sRNA reads

Species Read alignment
droSim3
AACAACA--------------ATTCAAATGCAAGCAA----AAAAAATAAAAAATAAAAAAGCTGAAGAGAATGATAGTGA-ATTGCTAAATATTTTCTTTGTATAAATGACTTTTAACATTTGTACAATATTAATATTTAAACATTTCTCGAATCAAAGTATGCCACATCAGCTTTGCAAGCCAAACGTGCGATGGACT----AGTGACGAAAGACACTAAAAGTSizeHit CountTotal NormTotalV044
Embryo		M023
Head		M024
Male-body		M025
Embryo		O001
Testis		O002
Head		M053
Female-body		SRR553485
Ovary		SRR553486
Ovary		SRR553487
Ovary		SRR553488
Ovary		SRR618934
Ovary
.......................................................................................TAAATATTTTCTTTGTATAA.......................................................................................................................20126.00260100250000000
.......................................................................................TAAATATTTTCTTTGTATA........................................................................................................................1916.006000060000000
.......................................................................................TAAATATTTTCTTTGTATAAA......................................................................................................................2114.004001030000000
.......................................................................................TAAATATTTTCTTTGTATAAATG....................................................................................................................2313.003001020000000
.........................................................................................................................TTGTACAATATTAATATT.......................................................................................1813.003000030000000
.......................................................................................TAAATATTTTCTTTGTAT.........................................................................................................................1813.003000030000000
........................................................................................................................TTTGTACAATATTAATATTTAAAC..................................................................................2412.002001010000000
.......................................................................................TAAATATTTTCTTTGTATAAAT.....................................................................................................................2212.002000010000010
.........................................................................................................................TTGTACAATATTAATATTTA.....................................................................................2012.002000020000000
.........................................................................................................................................................................................AACGTGCGATGGACT----AGTGACGAAAGA..........2711.001000000000001
..........................................................................................................................TGTACAATATTAATATTTAA....................................................................................2011.001000010000000
........................................................................................................................TTTGTACAATATTAATAT........................................................................................1821.002000020000000
..........................................................................................................................TGTACAATATTAATATTTA.....................................................................................1911.001000001000000
........................................................................................................................TTTGTACAATATTAATATTT......................................................................................2011.001001000000000
........................................................................................................................TTTGTACAATATTAATATTTA.....................................................................................2111.001000010000000
........................................................................................AAATATTTTCTTTGTATAA.......................................................................................................................1911.001000010000000
droSec2
AACAACA--------------ATTCAAATGCAAGCAATCTTAAAAA---AAAAATAAAAAAGCTGAAAAGAATGATAGTGA-ATTGCTAAATATTTTCTTTGTATAAATAACTTTTAATATTTGTACAATATTAATATTTAAACATTTCTCGAATCAAAGTATGCGACATCAGCTTTGCAAGCCAAACGTGCGATGGACT----AGTGACGAAAGACACTAAAAGTSizeHit CountTotal NormTotalV113
Male-body		V114
Embryo		V115
Head		M054
Female-body
.......................................................................................TAAATATTTTCTTTGTATAAAT.....................................................................................................................2212.0021001
.......................................................................................TAAATATTTTCTTTGTATAAATA....................................................................................................................2311.0011000

Generated: 12/06/2013 at 02:33 PM