ID:

dsi_131

 Coordinate:

3L:11184573-11184640 +

 Confidence:

Candidate

 Type:

Unknown

 [View on UCSC Genome Browser {Cornell Mirror}]
Legend: mature star mismatch in alignment mismatch in read

Predicted structure

-31.6 -31.6 -31.5

Flybase annnotation

intergenic

No Repeatable elements found

Sense Strand Reads

ATTTGAGCTCCTAGTCGAGCTCAGTAAATATTGGTGTTGCAAATCCAGGAAGGCTGAATCCAACTCTTCCCTATCCACCACGCTCTGCATGAGGCGAAAAGTCGCCTTCAGCCATTCCTGGAACGACATGGTGGGGCTGATAGGGTCTGTGTCCACTGGCGGGGATTC
***********************************((((....((((((((((((((.....................((.((...)).))((((((....)))))))))))).)))))))).))))......*********************************** 		Read size # Mismatch Hit Count Total Norm Total M023
head
 O002
Head
 SRR553486
Ovary		 M025
embryo
 SRR553487
Ovary		 SRR618934
Ovary
..................................................AGGCTGAATCCAACTCTTCCCT................................................................................................ 22 0 1 20.00 20 19 0 0 0 1 0
.................................................AAGGCTGAATCCAACTCTTCCC................................................................................................. 22 0 1 11.00 11 10 0 0 1 0 0
..................................................AGGCTGAATCCAACTCTTCCC................................................................................................. 21 0 1 5.00 5 5 0 0 0 0 0
....................................TTGCAAATCCAGGAAGGCTG................................................................................................................ 20 0 1 3.00 3 3 0 0 0 0 0
.................................................AAGGCTGAATCCAACTCTTCC.................................................................................................. 21 0 1 3.00 3 3 0 0 0 0 0
..................................................AGGCTGAATCCAACTCTTCCCC................................................................................................ 22 1 1 2.00 2 2 0 0 0 0 0
.................................................................................................AAAGTCGCCTTCAGCCATTCCT................................................. 22 0 1 2.00 2 0 2 0 0 0 0
........................................................................................................CCTTCAGCCATTCCTGGAACGA.......................................... 22 0 1 1.00 1 0 0 1 0 0 0
.................................GTGTTGCAAATCCAGGAAGGCTGAAT............................................................................................................. 26 0 1 1.00 1 1 0 0 0 0 0
.........................................AATCCAGGAAGGCTGAATCCAACTCT..................................................................................................... 26 0 1 1.00 1 0 0 1 0 0 0
.........................AAACATTGGTGTTGTAAATC........................................................................................................................... 20 2 1 1.00 1 0 0 0 0 0 0
................................................GAAGGCTGAATCCAACTCTTCC.................................................................................................. 22 0 1 1.00 1 1 0 0 0 0 0
..........................AACATTGGTGTTGTAAATC........................................................................................................................... 19 2 1 1.00 1 0 0 0 0 0 0
..................................................AGGCTGAATCCAACTCTTCCCG................................................................................................ 22 1 1 1.00 1 1 0 0 0 0 0
..................................................AGGCTGAATCCAACCCTTCCCT................................................................................................ 22 1 1 1.00 1 1 0 0 0 0 0
............................................................................................................CAGCCATTCCTTGAAAGA.......................................... 18 2 2 0.50 1 0 0 0 0 0 0
..................................................................................................................TTCCTGGAACGACAT....................................... 15 0 3 0.33 1 0 0 0 0 0 1
...........................ACATTGGTGTTGTAAATC........................................................................................................................... 18 2 6 0.17 1 0 0 0 0 0 0
.........................AAACATTGGTCTTGTAAATC........................................................................................................................... 20 3 13 0.15 2 0 0 0 0 0 0
.......................................................................................................................................................TCCACTGGCGTGGGTTC 17 2 15 0.07 1 0 0 0 0 0 1
...................................................................................................................................................TGTGTGCATTGGCGGGAATT. 20 3 18 0.06 1 0 0 1 0 0 0

Anti-sense strand reads

GAATCCCCGCCAGTGGACACAGACCCTATCAGCCCCACCATGTCGTTCCAGGAATGGCTGAAGGCGACTTTTCGCCTCATGCAGAGCGTGGTGGATAGGGAAGAGTTGGATTCAGCCTTCCTGGATTTGCAACACCAATATTTACTGAGCTCGACTAGGAGCTCAAAT
***********************************......((((.((((((((.((((((((((((....))))))((.((...)).)).....................))))))))))))))....))))*********************************** 		Read size # Mismatch Hit Count Total Norm Total SRR553486
Ovary		 SRR618934
Ovary
....................................................................TTTTCGCCTCATGCAGAGCGTGGTGG.......................................................................... 26 0 1 1.00 1 0 1
.........................................................................................................................................ATATTTACTGAGCTCGACTAG.......... 21 0 1 1.00 1 1 0
.............................................................................................................................TCTGGACCACCAATATTTACT...................... 21 3 6 0.50 3 0 0
..................................................CGAGTGGCTGAAGGCGAC.................................................................................................... 18 2 4 0.50 2 0 0
...........................................................................................TGGAAATGGAAGAGTTGGATC........................................................ 21 3 4 0.25 1 0 0
.................................................................................................................AGCCTTCCTGGCTTTGA...................................... 17 2 12 0.08 1 0 0
..........................................................................................GTGTATAGGGAAGAATT............................................................. 17 2 14 0.07 1 1 0
...........................................CGTCCCGGGAATGGCTGGA.......................................................................................................... 19 3 14 0.07 1 1 0
.................................................CCGAGTGGCTGAAGGCGAC.................................................................................................... 19 3 20 0.05 1 0 0

Re-alignment of all predicted orthologs

Species Coordinate ID Alignment
droSim3 3L:11184523-11184690 + dsi_131 ATTTGAGCT----CCTAGTCGAGCTCAGTAAATATTGGTGTTGCAAATCCAGGAAGGCTGAATCCAACTCTTCCCTATCCACCACGCTCTGCATGAGGCGAAAAGTCGCCTTCAGCCATTCCTGGAACGACATGGTGGGGCTGATAGGGTCTGTGTCCACTGGC-GGGGATTC
droSec2 scaffold_0:4004472-4004615 + ATTTGAGCT----CCTATCCGAGC------------------------TCAGGAAGGATGAATCCAACTCTTCCCTATCCACCACGCTCTGCATGAGGCGAAAAGTCGCCTTCAGCCATTCCTGGAACGACATAGTGGGGCTGATAGGGTCTGTGTCCACTGGC-GGGGATTC
dm3 chr3L:11819064-11819226 + TTTTGCGCTTTTTCCT----AATCTCAGTATATATTGTTGTTCCAAACCCAGCAAGGCCGAATCCAACTCTTTCTTATACACCGTGCTCTGCAAGAAGCGAAAAGTCGCCCTCAGGCATTCCTGGAACGACGTAGCGGGGCTTATATGGT------CCACTGGCAGGGGATTC

Re-alignment of all predicted orthologs with sRNA reads

Species Read alignment
droSim3
ATTTGAGCT----CCTAGTCGAGCTCAGTAAATATTGGTGTTGCAAATCCAGGAAGGCTGAATCCAACTCTTCCCTATCCACCACGCTCTGCATGAGGCGAAAAGTCGCCTTCAGCCATTCCTGGAACGACATGGTGGGGCTGATAGGGTCTGTGTCCACTGGC-GGGGATTCSizeHit CountTotal NormTotalV044
Embryo		M023
Head		M024
Male-body		M025
Embryo		O001
Testis		O002
Head		M053
Female-body		SRR553485
Ovary		SRR553486
Ovary		SRR553487
Ovary		SRR553488
Ovary		SRR618934
Ovary
......................................................AGGCTGAATCCAACTCTTCCCT.................................................................................................22120.00200190000000100
.....................................................AAGGCTGAATCCAACTCTTCCC..................................................................................................22111.00110100100000000
......................................................AGGCTGAATCCAACTCTTCCC..................................................................................................2115.005050000000000
........................................TTGCAAATCCAGGAAGGCTG.................................................................................................................2013.003030000000000
.....................................................AAGGCTGAATCCAACTCTTCC...................................................................................................2113.003030000000000
.....................................................................................................AAAGTCGCCTTCAGCCATTCCT..................................................2212.002000002000000
............................................................................................................CCTTCAGCCATTCCTGGAACGA...........................................2211.001000000001000
.....................................GTGTTGCAAATCCAGGAAGGCTGAAT..............................................................................................................2611.001010000000000
.............................................AATCCAGGAAGGCTGAATCCAACTCT......................................................................................................2611.001000000001000
....................................................GAAGGCTGAATCCAACTCTTCC...................................................................................................2211.001010000000000
......................................................................................................................TTCCTGGAACGACAT........................................1530.331000000000001
droSec2
ATTTGAGCT----CCTATCCGAGC------------------------TCAGGAAGGATGAATCCAACTCTTCCCTATCCACCACGCTCTGCATGAGGCGAAAAGTCGCCTTCAGCCATTCCTGGAACGACATAGTGGGGCTGATAGGGTCTGTGTCCACTGGC-GGGGATTCSizeHit CountTotal NormTotalV113
Male-body		V114
Embryo		V115
Head		M054
Female-body
No Reads

Generated: 12/06/2013 at 02:32 PM