ID:

dsi_124

 Coordinate:

X:942239-942299 -

 Confidence:

Candidate

 Type:

Unknown

 [View on UCSC Genome Browser {Cornell Mirror}]
Legend: mature star mismatch in alignment mismatch in read

Predicted structure

-37.8 -37.7 -37.7

Flybase annnotation

CDS [Dsim\GD16428-cds]; exon [dsim_GLEANR_16642:1]

No Repeatable elements found

Sense Strand Reads

-----------------------------------------------------------------------------------------------------------------------------------------------------############
 ATAATGAGCAACCTTTATACTCAAATGTTACATGTATGTATATATTCATGTCTTTTAGTACTTTGATCAGTAAATGTGTATTAATGTGCAGATCAAAGTACTAAAAGTCATGAATTCATACGATTTTTTTTTTAATATAAGTATGGAACATGCTTGCCATG
***********************************.(((((..(((((((.(((((((((((((((((.(((.((........)).))).))))))))))))))))).)))))))..)))))...************************************ 		Read size # Mismatch Hit Count Total Norm Total M023
head
 M025
embryo
 SRR553485
Ovary		 SRR618934
Ovary		 M024
male body
 O002
Head
 SRR553486
Ovary		 SRR553487
Ovary
..................................................TCTTTTAGTACTTTGATCAGTA......................................................................................... 22 0 1 11.00 11 3 5 0 2 0 0 0 1
..................................................TCTTTTAGTACTTCGATCAGTA......................................................................................... 22 1 1 6.00 6 0 0 0 0 0 0 2 1
..................................................TCTTTTAGTACTTTGATCAGT.......................................................................................... 21 0 1 3.00 3 2 0 0 0 1 0 0 0
........................................................................................CAGATCAAAGTACTAAAAGTCA................................................... 22 0 1 2.00 2 0 0 1 0 0 1 0 0
.....................................................................................TTGCAGATCAAAGTACTAAAA....................................................... 21 1 1 1.00 1 0 0 1 0 0 0 0 0
....................................................TTTTAGTACTTTGATCAGTAAATGTGTA................................................................................. 28 0 1 1.00 1 0 0 1 0 0 0 0 0
................................................TGTCTTTTAGTACTTTGATCAGT.......................................................................................... 23 0 1 1.00 1 1 0 0 0 0 0 0 0
................................................TGTCTTTTAGTACTTTGATCAGTAAA....................................................................................... 26 0 1 1.00 1 0 0 1 0 0 0 0 0
..................................................TCTTTTAGTACTTCGATCAGT.......................................................................................... 21 1 1 1.00 1 0 0 0 0 0 0 1 0
......................................................................................TGCAGATCAAAGTACTAAAAGTCATG................................................. 26 0 1 1.00 1 0 0 1 0 0 0 0 0
.....................................................................................................................ATACGATTTTTTTTTT............................ 16 0 2 1.00 2 0 0 0 0 0 0 2 0
.....................................................................................................................ATACGATTTTTTTTT............................. 15 0 9 0.33 3 0 0 3 0 0 0 0 0
..................................................GCTTTTAGTACTTTGATG............................................................................................. 18 2 11 0.09 1 0 0 0 0 0 0 0 0
.....................................................................................GTGCAGATCAAATTA............................................................. 15 1 19 0.05 1 0 0 0 0 0 0 1 0

Anti-sense strand reads

-----------------------------------------------------------------------------------------------------------------------------------------------------############
 CATGGCAAGCATGTTCCATACTTATATTAAAAAAAAAATCGTATGAATTCATGACTTTTAGTACTTTGATCTGCACATTAATACACATTTACTGATCAAAGTACTAAAAGACATGAATATATACATACATGTAACATTTGAGTATAAAGGTTGCTCATTAT
************************************...(((((..(((((((.(((((((((((((((((.(((.((........)).))).))))))))))))))))).)))))))..))))).*********************************** 		Read size # Mismatch Hit Count Total Norm Total SRR553485
Ovary		 M023
head
 GSM343915
embryo
.....................................................ACTTTTAGTACTTTGATCTGC....................................................................................... 21 0 1 3.00 3 3 0 0
...........TGTTACATACTTATAAAAAAAAAAAAA........................................................................................................................... 27 3 1 1.00 1 0 0 0
.........................................................................................TACTGATCAAAGTACTAAAAGACA................................................ 24 0 1 1.00 1 1 0 0
............................................GAATTCATGACTTTTAGTACTT............................................................................................... 22 0 1 1.00 1 0 1 0
............................................................................................TGATCAAAGTACTAAAAGACATGA............................................. 24 0 1 1.00 1 0 0 1
........................................................................................................................................TTTGGGTATAAGGGTGGCTC..... 20 3 4 0.50 2 0 0 0
..........................................ATGAACTCATAACTTTTAGTA.................................................................................................. 21 2 2 0.50 1 0 0 0
.....................................................GCTTTTAGTACTTTGATG.......................................................................................... 18 2 11 0.09 1 0 0 0

Re-alignment of all predicted orthologs

Species Coordinate ID Alignment
droSim3 X:942189-942349 - dsi_124 ATAATGAGCAACCTTTATACTCAAATGTTACATG--TATGTATA--TATTCATGTCTTTTAGTACTTTGATCAGTAAATGTGTATTAATGTGCAGAT-----------------------------------CAAAGTACTAAAAGTCATGAATTCATACGATT-TTTTTTTTAATATAAGTATGGAACATGCTTGCCATG
droSec2 scaffold_10:1042292-1042455 - ATAATGAGTAACCTTTATACTCAAATGTTATATG--TATGTATATATATTCATGTCTTTTAGTACTTTGATCAGTAAATGTGTATTAATTTGCAGAT-----------------------------------CAAAGTACTAAAAGTCATGAATTCATACGTTTTTTTTTTTTAATATAAGTATGGAACATGCTTGCCATG
dm3 chrX:1209202-1209287 - TTTGCA--------------------------------------------------------------------------------CATTTGCTGAT-----------------------------------CAAAATACTAAACGACATAATTTCATACTTTT-TTTTTTTTAATATAAGTATGGAACATGCTTACCATG
droEre2 scaffold_4644:1200979-1201094 - AAAATGTGTCACACTTGTATTCAAATATTGTGGAAATCATAATAAGCA-------CT------------------------GC----------------------------TGATATTTTGTTAGACCACGTCCATGTACTAAATGCGATGAACTCCCT------CTTGTTTGGGAATGAA--------------------
droYak3 X:1126910-1127058 - ATAGTATGCCACAT-------AACATGTCACAAA--TAGTGATAAGCA-------TT------------------------GTGGTAATGTGCTGGCCAAGCCATTTCAAATACTCTTTTGCTAGACGATGTCAATGTACCAAAGGAGCTGCATTCCCT------CTTGTTTGGGAATACATTCGAAACATG------ATG

Re-alignment of all predicted orthologs with sRNA reads

Species Read alignment
droSim3
ATAATGAGCAACCTTTATACTCAAATGTTACATG--TATGTATA--TATTCATGTCTTTTAGTACTTTGATCAGTAAATGTGTATTAATGTGCAGAT-----------------------------------CAAAGTACTAAAAGTCATGAATTCATACGATT-TTTTTTTTAATATAAGTATGGAACATGCTTGCCATGSizeHit CountTotal NormTotalV044
Embryo		M023
Head		M024
Male-body		M025
Embryo		O001
Testis		O002
Head		M053
Female-body		SRR553485
Ovary		SRR553486
Ovary		SRR553487
Ovary		SRR553488
Ovary		SRR618934
Ovary
......................................................TCTTTTAGTACTTTGATCAGTA.............................................................................................................................22111.0011030500000102
......................................................TCTTTTAGTACTTTGATCAGT..............................................................................................................................2113.003021000000000
............................................................................................CAGAT-----------------------------------CAAAGTACTAAAAGTCA....................................................2212.002000001010000
........................................................TTTTAGTACTTTGATCAGTAAATGTGTA.....................................................................................................................2811.001000000010000
....................................................TGTCTTTTAGTACTTTGATCAGT..............................................................................................................................2311.001010000000000
....................................................TGTCTTTTAGTACTTTGATCAGTAAA...........................................................................................................................2611.001000000010000
..........................................................................................TGCAGAT-----------------------------------CAAAGTACTAAAAGTCATG..................................................2611.001000000010000
............................................................................................................................................................ATACGATT-TTTTTTTT............................1621.002000000002000
............................................................................................................................................................ATACGATT-TTTTTTT.............................1590.333000000030000
droSec2
ATAATGAGTAACCTTTATACTCAAATGTTATATG--TATGTATATATATTCATGTCTTTTAGTACTTTGATCAGTAAATGTGTATTAATTTGCAGAT-----------------------------------CAAAGTACTAAAAGTCATGAATTCATACGTTTTTTTTTTTTAATATAAGTATGGAACATGCTTGCCATGSizeHit CountTotal NormTotalV113
Male-body		V114
Embryo		V115
Head		M054
Female-body
No Reads
droEre2
AAAATGTGTCACACTTGTATTCAAATATTGTGGAAATCATAATAAGCA-------CT------------------------GC----------------------------TGATATTTTGTTAGACCACGTCCATGTACTAAATGCGATGAACTCCCT------CTTGTTTGGGAATGAA--------------------SizeHit CountTotal NormTotalV040
Embryo		V060
Head		V107
Male-body		V108
Head		M055
Female-body		M057
Embryo
No Reads
droYak3
ATAGTATGCCACAT-------AACATGTCACAAA--TAGTGATAAGCA-------TT------------------------GTGGTAATGTGCTGGCCAAGCCATTTCAAATACTCTTTTGCTAGACGATGTCAATGTACCAAAGGAGCTGCATTCCCT------CTTGTTTGGGAATACATTCGAAACATG------ATGSizeHit CountTotal NormTotalV046
Embryo		V052
Head		V120
Male-body		V058
Head		M026
Head		M043
Female-body		M056
Embryo
No Reads

Generated: 12/06/2013 at 02:31 PM