dsi_109

########################################################################################################################################################################### CATGATGAGCCCGGAGATCGAGCAGTTCAGCAACTCAGTGCCAAAATCGCCCATTCGGGAGCTGCTCGAGCTGGAGCCGGAAACGGCCAAGTTCGGCAAGCCCGAGAAGCTGGCGGATGGGCGACGGGTGCGCGTTACCGTGGATGTCTTCTGCAAAGGAACCTTCCGTGG *********************************..(((((....(((((((((((..((((.((((.(((...(((((..((......))))))).))).)))).))))..)))))))))))..))))).....*********************************	Read size	# Mismatch	Hit Count	Total Norm	Total	SRR618934 Ovary	M023 head	SRR553487 Ovary	SRR553485 Ovary	M024 male body	SRR553486 Ovary	SRR553488 Ovary	M025 embryo	M053 Female-body	O001 Testis	O002 Head	GSM343915 embryo
....................................................................................................CCCGAGAAGCTGGCGGATGGGC.................................................	22	0	1	13.00	13	8	2	0	3	0	0	0	0	0	0	0	0
....................................................................................................CCCGAGAAGCTGGCGGATGGG..................................................	21	0	1	11.00	11	4	2	4	1	0	0	0	0	0	0	0	0
....................................................................................................CCCGAGAAGCTGGCGGATGG...................................................	20	0	1	9.00	9	1	4	1	0	3	0	0	0	0	0	0	0
....................................................................................................CCCGAGAAGCTGGCGGATGGA..................................................	21	1	1	7.00	7	0	0	3	0	0	2	2	0	0	0	0	0
....................................................................................................CCCGAGAAGCTGGCGGATGGGG.................................................	22	1	1	5.00	5	0	0	2	0	0	0	0	0	0	0	0	3
.................................................CCCATTCGGGAGCTGCTCGAG.....................................................................................................	21	0	1	4.00	4	0	0	1	0	0	2	1	0	0	0	0	0
.....................................................................................................CCGAGAAGCTGGCGGATG....................................................	18	0	1	2.00	2	0	1	0	0	0	0	0	0	0	1	0	0
....................................................................................................CCCGAGAAGCTGGCGGATG....................................................	19	0	1	2.00	2	0	0	0	0	1	0	0	0	0	0	1	0
.................................................CCCATTCGGGAGCTGCTCGAGC....................................................................................................	22	0	1	2.00	2	0	0	1	0	0	0	1	0	0	0	0	0
..................................................CCATTCGGGAGCTGCTCGAGCTT..................................................................................................	23	1	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0
....................................................................................................CCCGAGAAGCTGGCGGATGGGT.................................................	22	1	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
..................................................CCATTCGGGAGCTGCTCGAGCT...................................................................................................	22	0	1	1.00	1	0	0	0	0	0	0	0	1	0	0	0	0
.................................................CCCATTCGGGAGCTGCTCGA......................................................................................................	20	0	1	1.00	1	0	0	0	0	0	1	0	0	0	0	0	0
....................................................................................................CCCGAGAAGCTGGCGGATGGGCTT...............................................	24	2	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
....................................................................................................CCCGAGAAGCGGGCGGATGGGG.................................................	22	2	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	1
..................................................CCATTCGGGAGCTGCTCTAGA....................................................................................................	21	2	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0
....................................................................................................CCCGAGAAGCTGGCGGATGGTT.................................................	22	2	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	1
.....................................................................................................CCGAGAAGCTGGCGGATGGG..................................................	20	0	1	1.00	1	0	0	0	0	0	0	0	0	1	0	0	0
.........................TTCAGCAACTCAGTGCCAAAATCGCCCATT....................................................................................................................	30	0	1	1.00	1	0	0	0	0	0	1	0	0	0	0	0	0
.....................................................................................................CCGAGAAGCTGGCGGATGGGG.................................................	21	1	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0
..................................................CCATTCGGGAGCTGCTCGAGC....................................................................................................	21	0	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	1
....................................................................................................CCCGAGAAGCTGGCGGATGGGCCA...............................................	24	1	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	1
.....................................................................................................CCGAGAAGCTGGCGGATGG...................................................	19	0	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0
..................................................CCATTCGGGAGCTGCTCGA......................................................................................................	19	0	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0
....................................................................................................CCCGAGAAGCTGGCGGATGGAC.................................................	22	1	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0
....................................................................................................CCCGAGAAGCTGTCGGATGG...................................................	20	1	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0
......................................................................................................CGAGAAGCTGGCGGATGGGCG................................................	21	0	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
..........................................................................AGCCGGAAACGGCCAAGTTCGGCAAGCC.....................................................................	28	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0
.....................................................................................................................................GTTACCGTGGATGTCTTCTGCAAAGGA...........	27	0	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0
......................................................................................................................GGGCGACGGGTGCGCGT....................................	17	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0
.....................................................................................................CCGAGAAGCTGGCGGATGGGC.................................................	21	0	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0
....................................................................................................CCCGAGAAGCTGGCGGTTGGGC.................................................	22	1	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0
.....................................................................................................CCGAGAAGCTGGCGGATGT...................................................	19	1	2	0.50	1	0	1	0	0	0	0	0	0	0	0	0	0
.....................................................................................................................................GTTCCCGTGGATGTCGGCT...................	19	3	13	0.15	2	0	0	0	0	0	0	0	2	0	0	0	0
...................................................CCTTCGGGAGCAGCTCGGG.....................................................................................................	19	3	15	0.13	2	0	0	2	0	0	0	0	0	0	0	0	0
........................................................................GGAGCCGGAAACGGT....................................................................................	15	1	19	0.05	1	0	0	0	0	0	0	1	0	0	0	0	0
...........................................................CGCGGCTCGAGCTGGAG...............................................................................................	17	2	20	0.05	1	1	0	0	0	0	0	0	0	0	0	0	0
........................................................................GGAGCCGGAAACGGTAA..................................................................................	17	2	20	0.05	1	0	0	0	0	0	0	1	0	0	0	0	0

########################################################################################################################################################################### CCACGGAAGGTTCCTTTGCAGAAGACATCCACGGTAACGCGCACCCGTCGCCCATCCGCCAGCTTCTCGGGCTTGCCGAACTTGGCCGTTTCCGGCTCCAGCTCGAGCAGCTCCCGAATGGGCGATTTTGGCACTGAGTTGCTGAACTGCTCGATCTCCGGGCTCATCATG *********************************.....(((((..(((((((((((..((((.((((.(((.(((((((......))..)))))...))).)))).))))..)))))))))))....)))))..*********************************	Read size	# Mismatch	Hit Count	Total Norm	Total
..................................................................TCGGGCTTGCCGAACTTGGCCT...................................................................................	22	1	1	1.00	1
.....GAAGAATCCTTTGCAGACGA..................................................................................................................................................	20	3	12	0.33	4
...............................CAGTAACGCGCACCA.............................................................................................................................	15	2	20	0.25	5
..............................................ATCGCCCATGCGCCAGCT...........................................................................................................	18	2	8	0.13	1
.......................................................................CATCCCGAACTCGGCCGTT.................................................................................	19	3	9	0.11	1
.....GAAGAATCCTTTGCAGA.....................................................................................................................................................	17	2	20	0.10	2
......................................GTGCACCGGTCGTCCATCC..................................................................................................................	19	3	11	0.09	1
........................................................................................................................................AGCTGCTGAACTACTTGATC...............	20	3	17	0.06	1
.....................................................................................CCGTTTCCCGCTCCA.......................................................................	15	1	20	0.05	1

Re-alignment of all predicted orthologs

Species	Coordinate	ID	Alignment
droSim3	3R:18370815-18370985 -	dsi_109	CATGATGAGCCCGGAGATCGAGCAGTTCAGCAACTCAGTGCCAAAATCGCCCATTCGGGAGCTGCTCGAGCTGGAGCCGGAAACGGCCAAGTTCGGCAAGCCCGAGAAGCTGGCGGATGGGCGACGGGTGCGCGTTACCGTGGATGTCTTCTGCAAAGGAACCTTCCGTGG
droSec2	scaffold_0:18916384-18916554 -		CATGATGAGCCCGGAGATCGAGCAGTTCAGCAACTCAGTGCCAAAATCGCCTATTCGGGAGCTGCTCGAGCTGGAGCCGGAAACGGCCAAGTTCGGCAAACCCGAGAAGCTGGCGGATGGGCGACGGGTGCGCGTTACCGTGGATGTCTTCTGCAAAGGAACCTTCCGTGG
dm3	chr3R:18559925-18560095 -		CATGATGAGCCCGGAGATCGAGCAGTTCAGCAACTCAGTGCCAAAATCGCCCATTCGGGAGCTCCTCGAGCTGGAGCCGGAAACCGCCAAGTTCGGCAAGCCCGAGAAGCTGGCGGATGGGCGACGGGTGCGCGTTACCGTGGATGTCTTCTGCAAAGGAACCTTCCGTGG
droEre2	scaffold_4820:9513201-9513371 +		CATGATGAGCCCGGAGATCGAACAGTTCAGCAACTCAGTGCCAAAATCGCCCATTCGGGAGCTGCTCGAGCTGGAGCCGGAAACAGCCAAATTCGGCAAGCCCGAGAAGCTGGCGGATGGGCGACGGGTGCGCGTTACCGTGGATGTCTTCTGCAAAGGAACCTTCCGTGG
droYak3	3R:19409947-19410117 -		CATGATGAGCCCGGAGATCGAGCAGTTCAGCAACTCAGTGCCAAAATCGCCCATTCGGGAGCTGCTCGAGCTGGAGCCGGAGACAGCCAAATTCGGCAAGCCCGAGAAGCTGGCGGATGGGCGACGGGTGCGCGTTACCGTGGATGTCTTCTGCAAGGGAACCTTCCGTGG
droEug1	scf7180000409768:704122-704292 -		TATGATGAGTCCGGAAATTGAGCAATTTAGCAACTCAGTGCCAAAGTCGCCCATACGGGAACTCCTCGAGCTAGAACCGGAGACAGCCAAGTTCGGTAAGCCCGAGAAGCTGGCGGATGGGCGACGGGTGCGCGTTACCGTGGATGTCTTCTGCAAAGGAACCTTCCGTGG
droBia1	scf7180000302402:5151775-5151945 +		CATGATGAGCCCGGAGATAGAGCAGTTCAGTAACTCAGTGCCAAAGTCGCCCATCCGGGAGCTCCTCGAGCTGGAGCCGGAGACAGCCAAGTTCGGCAAGCCCGAGAAGCTGGCGGACGGGCGGCGGGTGCGCGTCACCGTGGATGTGTTCTGCAAAGGAACCTTCCGTGG
droTak1	scf7180000415417:96871-97041 +		CATGATGAGCCCGGAGATTGAACAGTTTAGCAACTCAGTGCCAAAGTCGCCCATACGGGAGCTGCTCGAGCTGGAACCGGAGACAGCCAAGTTCGGGAAGCCCGAGAAGCTGGCGGATGGGCGGCGGGTGCGCGTTACCGTGGATGTCTTCTGCAAAGGAACCTTCCGTGG
droEle1	scf7180000491017:254771-254941 -		CATGATGAGCCCGGAGATCGAGCAGTTTAGCAACTCAGTGCCAAAGTCGCCCATAAGGGAGCTCCTCGAGCTGGAGCCGGAGACGGCCAAGTTCGGTAAGCCCGAGAAGCTGGCGGACGGGCGGCGGGTTCGCGTCACCGTGGATGTCTTCTGCAAGGGCACCTTCCGGGG
droRho1	scf7180000777285:53295-53465 +		CATGATGAGCCCAGAGATTGAACAATTTAGCAACTCAGTGCCAAAGTCGCCCATACGAGAGCTCCTCGAGCTGGAGCCGGAGACGGCCAAGTTCGGCAAGCCCGAGAAGCTGGCGGATGGGCGGCGTGTGCGCGTCACCGTGGATGTATTTTGCAAAGGCACCTTCCGTGG
droFic1	scf7180000454055:259477-259647 +		TATGATGAGTCCGGAGATCGAACAATTCAGCAACTCAGTGCCAAAGTCGCCCATCCGGGAGCTCCTCGAACTGGAGCCGGAGACGGCCAAATTCGGCAAGCCTGAGAAGCTGGCTGATGGGCGACGGGTGCGCGTCACCGTGGATGTCTTCTGCAAGGGCACCTTCCGCGG
droKik1	scf7180000302697:1118492-1118662 -		TATGATGAGCCCGGAAATTGAACAGTTTAGCAATTCAGTGCCAAAGTCGCCCATTCGGGAGCTGCTCGAGCTGGAGCCGGAGACGGCCAAGTTTGGCAAGCCGGAGAAACTGGCCGATGGACGTCGCGTGCGCGTCACCGTCGATGTCTTCTGCAAGGGAACCTTCCGTGG
droAna3	scaffold_13340:12755642-12755812 +		TATGATGAGTCCGGAGATAGAGCAATTCAGCAACTCTGTGCCAAAGTCGCCCATACGGGAGCTCCTTGAACTGGAACCGGAGACGGCCAAGTTCGGCAAGCCGGAGAAACTGGCCGACGGACGCCGGGTTCGCGTCACCGTCGACGTGTTCTGCAAGGGCACCTTCCGCGG
droBip1	scf7180000396691:1641758-1641928 +		TATGATGAGTCCGGAGATTGAGCAGTTTAGTAACTCAGTGCCAAAGTCGCCGATACGGGAGCTCCTTGAACTGGAACCGGAGACCGCCAAGTTCGGCAAGCCGGAGAAGCTGGCCGACGGTCGCCGGGTTCGCGTCACCGTCGATGTGTTCTGCAAGGGCACCTTCCGCGG
dp5	2:6273971-6274141 +		TATGATGAGCCCGGAAATCGAGCAGTTCAGCAACTCAGTGCCAAAGTCCCCTATACGGGAACTGCTCGAACTGGAGCCGGAGACGGCCAAATTTGGGAAGCCGGAGAAGCTGGCCGACGGACGCCGGGTGCGCGTCACCGTCGATGTCTTCTGCAAGGGCACCTTCCGTGG
droPer2	scaffold_0:98164-98334 +		TATGATGAGCCCGGAAATCGAGCAGTTCAGCAACTCAGTGCCAAAGTCCCCCATACGGGAACTGCTCGAACTGGAGCCGGAGACGGCCAAATTTGGGAAGCCGGAGAAGCTGGCCGACGGACGCCGGGTGCGCGTCACCGTCGATGTCTTCTGCAAGGGCACCTTCCGTGG
droWil2	scf2_1100000004902:1472426-1472596 +		CATGATGAAGCCTGAAATCGAACAATTCAGCAACTCAGTGCCAAAATCACCCATACGGGAACTTCTCGAACTGGAGCCCGAGACGGCCAAGTTTGGTAAACCCGAGAAATTGGCCGATGGTCGGCGGGTCCGTGTCACAGTCGATGTCTTCTGCAAGGGCACTTTCCGTGG
droVir3	scaffold_13047:13210686-13210856 +		TATGATGAAGCCCGAGATCGAGCAATTTAGTAATTCTGTGCCAAAATCACCCATACGAGAGCTGCTCGAGCTGGAACCGGAGACGGCCAAGTTTGGTAAGCCCGAGAAGTTGGCCGATGGTCGTCGTGTACGCGTCACTGTCGATGTCTTCTGCAAGGGCACATTCCGTGG
droMoj3	scaffold_6540:10445719-10445889 +		TATGATGAAGCCCGAGATAGAGCAGTTTAGCAATTCTGTGCCAAAGTCACCCATACGAGAGCTGCTCGAGCTGGAGCCGGAAACGGCCAAATTTGGCAAGCCAGAGAAACTGGCGGACGGACGTCGCGTTCGCGTCACTGTTGATGTCTTTTGCAAGGGCACGTTCCGCGG
droGri2	scaffold_14906:4481380-4481550 -		CATGATGAAGCCGGAGATAGAACAATTTAGCAATTCTGTGCCAAAGTCACCCATTCGCGAGCTGCTCGAGCTCGAACCAGAAACGGCCAAGTTCGGCAAGCCAGAGAAGCTGGCAGACGGTCGCCGCGTACGCGTCACTGTGGATGTCTTCTGCAAGGGCACGTTCCGTGG

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droSim3

CATGATGAGCCCGGAGATCGAGCAGTTCAGCAACTCAGTGCCAAAATCGCCCATTCGGGAGCTGCTCGAGCTGGAGCCGGAAACGGCCAAGTTCGGCAAGCCCGAGAAGCTGGCGGATGGGCGACGGGTGCGCGTTACCGTGGATGTCTTCTGCAAAGGAACCTTCCGTGG	Size	Hit Count	Total Norm	Total	V044 Embryo	M023 Head	M024 Male-body	M025 Embryo	O001 Testis	O002 Head	M053 Female-body	SRR553485 Ovary	SRR553486 Ovary	SRR553487 Ovary	SRR553488 Ovary	SRR618934 Ovary
....................................................................................................CCCGAGAAGCTGGCGGATGGGC.................................................	22	1	13.00	13	0	2	0	0	0	0	0	3	0	0	0	8
....................................................................................................CCCGAGAAGCTGGCGGATGGG..................................................	21	1	11.00	11	0	2	0	0	0	0	0	1	0	4	0	4
....................................................................................................CCCGAGAAGCTGGCGGATGG...................................................	20	1	9.00	9	0	4	3	0	0	0	0	0	0	1	0	1
.................................................CCCATTCGGGAGCTGCTCGAG.....................................................................................................	21	1	4.00	4	0	0	0	0	0	0	0	0	2	1	1	0
.....................................................................................................CCGAGAAGCTGGCGGATG....................................................	18	1	2.00	2	0	1	0	0	1	0	0	0	0	0	0	0
....................................................................................................CCCGAGAAGCTGGCGGATG....................................................	19	1	2.00	2	0	0	1	0	0	1	0	0	0	0	0	0
.................................................CCCATTCGGGAGCTGCTCGAGC....................................................................................................	22	1	2.00	2	0	0	0	0	0	0	0	0	0	1	1	0
..................................................CCATTCGGGAGCTGCTCGAGCT...................................................................................................	22	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0
.................................................CCCATTCGGGAGCTGCTCGA......................................................................................................	20	1	1.00	1	0	0	0	0	0	0	0	0	1	0	0	0
.....................................................................................................CCGAGAAGCTGGCGGATGGG..................................................	20	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0	0
.........................TTCAGCAACTCAGTGCCAAAATCGCCCATT....................................................................................................................	30	1	1.00	1	0	0	0	0	0	0	0	0	1	0	0	0
..................................................CCATTCGGGAGCTGCTCGAGC....................................................................................................	21	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0
.....................................................................................................CCGAGAAGCTGGCGGATGG...................................................	19	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0
..................................................CCATTCGGGAGCTGCTCGA......................................................................................................	19	1	1.00	1	0	0	0	0	0	0	0	1	0	0	0	0
......................................................................................................CGAGAAGCTGGCGGATGGGCG................................................	21	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0
..........................................................................AGCCGGAAACGGCCAAGTTCGGCAAGCC.....................................................................	28	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	1
.....................................................................................................................................GTTACCGTGGATGTCTTCTGCAAAGGA...........	27	1	1.00	1	0	0	0	0	0	0	0	1	0	0	0	0
......................................................................................................................GGGCGACGGGTGCGCGT....................................	17	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	1
.....................................................................................................CCGAGAAGCTGGCGGATGGGC.................................................	21	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0

droSec2

CATGATGAGCCCGGAGATCGAGCAGTTCAGCAACTCAGTGCCAAAATCGCCTATTCGGGAGCTGCTCGAGCTGGAGCCGGAAACGGCCAAGTTCGGCAAACCCGAGAAGCTGGCGGATGGGCGACGGGTGCGCGTTACCGTGGATGTCTTCTGCAAAGGAACCTTCCGTGG	Size	Hit Count	Total Norm	Total	V113 Male-body	V114 Embryo	V115 Head	M054 Female-body
..TGATGAGCCCGGAGATCGAG.....................................................................................................................................................	20	1	1.00	1	0	0	0	1
.ATGATGAGCCCGGAGATCGAGC....................................................................................................................................................	22	1	1.00	1	1	0	0	0
.............................................ATCGCCTATTCGGGAGCTGCTCGA......................................................................................................	24	1	1.00	1	1	0	0	0
........................................................................................AAGTTCGGCAAACCCGAGAAGCTGG..........................................................	25	1	1.00	1	1	0	0	0
..........................................................................................GTTCGGCAAACCCGAGAAGCTGGC.........................................................	24	1	1.00	1	1	0	0	0
..................................................................................................AACCCGAGAAGCTGGCGGATGGG..................................................	23	1	1.00	1	0	1	0	0
......................................................................................................CGAGAAGCTGGCGGATGG...................................................	18	1	1.00	1	0	0	0	1
................................................................................................CAAACCCGAGAAGCTGGCGG.......................................................	20	1	1.00	1	1	0	0	0
.......................................GCCAAAATCGCCTATTCGGGAGC.............................................................................................................	23	1	1.00	1	0	1	0	0

droEre2

CATGATGAGCCCGGAGATCGAACAGTTCAGCAACTCAGTGCCAAAATCGCCCATTCGGGAGCTGCTCGAGCTGGAGCCGGAAACAGCCAAATTCGGCAAGCCCGAGAAGCTGGCGGATGGGCGACGGGTGCGCGTTACCGTGGATGTCTTCTGCAAAGGAACCTTCCGTGG	Size	Hit Count	Total Norm	Total	V040 Embryo	V060 Head	V107 Male-body	V108 Head	M055 Female-body	M057 Embryo
...............................................CGCCCATTCGGGAGCTGCTCGAGCTGG.................................................................................................	27	1	1.00	1	0	0	1	0	0	0
...............................AACTCAGTGCCAAAATCGCCCATTCGGGA...............................................................................................................	29	1	1.00	1	0	0	0	0	0	1
.ATGATGAGCCCGGAGATCGAACA...................................................................................................................................................	23	1	1.00	1	0	0	0	0	1	0
......................................................TCGGGAGCTGCTCGAGCTGGAGCC.............................................................................................	24	1	1.00	1	0	0	1	0	0	0
...................................................................................................................................................CTTCTGCAAAGGAACCTTCCG...	21	1	1.00	1	0	0	0	0	1	0

droYak3

CATGATGAGCCCGGAGATCGAGCAGTTCAGCAACTCAGTGCCAAAATCGCCCATTCGGGAGCTGCTCGAGCTGGAGCCGGAGACAGCCAAATTCGGCAAGCCCGAGAAGCTGGCGGATGGGCGACGGGTGCGCGTTACCGTGGATGTCTTCTGCAAGGGAACCTTCCGTGG	Size	Hit Count	Total Norm	Total	V046 Embryo	V052 Head	V120 Male-body	V058 Head	M026 Head	M043 Female-body	M056 Embryo
.................................................................................GACAGCCAAATTCGGCAAGC......................................................................	20	1	1.00	1	0	0	0	0	0	0	1
........................................................................................................................................................GCAAGGGAACCTTCCGTGG	19	1	1.00	1	0	0	1	0	0	0	0
...GATGAGCCCGGAGATCGAGC....................................................................................................................................................	20	1	1.00	1	1	0	0	0	0	0	0
.ATGATGAGCCCGGAGATCGAGCAGTT................................................................................................................................................	26	1	1.00	1	0	0	1	0	0	0	0
..............................................................TGCTCGAGCTGGAGCCGGAGACAGC....................................................................................	25	1	1.00	1	1	0	0	0	0	0	0

droEug1

TATGATGAGTCCGGAAATTGAGCAATTTAGCAACTCAGTGCCAAAGTCGCCCATACGGGAACTCCTCGAGCTAGAACCGGAGACAGCCAAGTTCGGTAAGCCCGAGAAGCTGGCGGATGGGCGACGGGTGCGCGTTACCGTGGATGTCTTCTGCAAAGGAACCTTCCGTGG	Size	Hit Count	Total Norm	Total
No Reads

droBia1

CATGATGAGCCCGGAGATAGAGCAGTTCAGTAACTCAGTGCCAAAGTCGCCCATCCGGGAGCTCCTCGAGCTGGAGCCGGAGACAGCCAAGTTCGGCAAGCCCGAGAAGCTGGCGGACGGGCGGCGGGTGCGCGTCACCGTGGATGTGTTCTGCAAAGGAACCTTCCGTGG	Size	Hit Count	Total Norm	Total
No Reads

droTak1

CATGATGAGCCCGGAGATTGAACAGTTTAGCAACTCAGTGCCAAAGTCGCCCATACGGGAGCTGCTCGAGCTGGAACCGGAGACAGCCAAGTTCGGGAAGCCCGAGAAGCTGGCGGATGGGCGGCGGGTGCGCGTTACCGTGGATGTCTTCTGCAAAGGAACCTTCCGTGG	Size	Hit Count	Total Norm	Total
No Reads

droEle1

CATGATGAGCCCGGAGATCGAGCAGTTTAGCAACTCAGTGCCAAAGTCGCCCATAAGGGAGCTCCTCGAGCTGGAGCCGGAGACGGCCAAGTTCGGTAAGCCCGAGAAGCTGGCGGACGGGCGGCGGGTTCGCGTCACCGTGGATGTCTTCTGCAAGGGCACCTTCCGGGG	Size	Hit Count	Total Norm	Total
No Reads

droRho1

CATGATGAGCCCAGAGATTGAACAATTTAGCAACTCAGTGCCAAAGTCGCCCATACGAGAGCTCCTCGAGCTGGAGCCGGAGACGGCCAAGTTCGGCAAGCCCGAGAAGCTGGCGGATGGGCGGCGTGTGCGCGTCACCGTGGATGTATTTTGCAAAGGCACCTTCCGTGG	Size	Hit Count	Total Norm	Total
No Reads

droFic1

TATGATGAGTCCGGAGATCGAACAATTCAGCAACTCAGTGCCAAAGTCGCCCATCCGGGAGCTCCTCGAACTGGAGCCGGAGACGGCCAAATTCGGCAAGCCTGAGAAGCTGGCTGATGGGCGACGGGTGCGCGTCACCGTGGATGTCTTCTGCAAGGGCACCTTCCGCGG	Size	Hit Count	Total Norm	Total
No Reads

droKik1

TATGATGAGCCCGGAAATTGAACAGTTTAGCAATTCAGTGCCAAAGTCGCCCATTCGGGAGCTGCTCGAGCTGGAGCCGGAGACGGCCAAGTTTGGCAAGCCGGAGAAACTGGCCGATGGACGTCGCGTGCGCGTCACCGTCGATGTCTTCTGCAAGGGAACCTTCCGTGG	Size	Hit Count	Total Norm	Total
No Reads

droAna3

TATGATGAGTCCGGAGATAGAGCAATTCAGCAACTCTGTGCCAAAGTCGCCCATACGGGAGCTCCTTGAACTGGAACCGGAGACGGCCAAGTTCGGCAAGCCGGAGAAACTGGCCGACGGACGCCGGGTTCGCGTCACCGTCGACGTGTTCTGCAAGGGCACCTTCCGCGG	Size	Hit Count	Total Norm	Total	V105 Male-body	V106 Head	V055 Head	V039 Embryo	M044 Female-body	M058 Embryo
.....................................................................................................................................................TCTGCAAGGGCACCTTCCGCG.	21	1	2.00	2	0	2	0	0	0	0
..........................................................................................................AAACTGGCCGACGGACGCCGG............................................	21	1	1.00	1	0	0	0	0	1	0
...................................................................................................GCCGGAGAAACTGGCCGACGGACGC...............................................	25	1	1.00	1	0	0	0	0	1	0
...GATGAGTCCGGAGATAGAGCA...................................................................................................................................................	21	1	1.00	1	0	0	0	0	1	0
............................................................................................................................................TCGACGTGTTCTGCAAGGGCA..........	21	1	1.00	1	1	0	0	0	0	0
.......................................................................................................GAGAAACTGGCCGACGGACGCCGGGTT.........................................	27	1	1.00	1	1	0	0	0	0	0

droBip1

TATGATGAGTCCGGAGATTGAGCAGTTTAGTAACTCAGTGCCAAAGTCGCCGATACGGGAGCTCCTTGAACTGGAACCGGAGACCGCCAAGTTCGGCAAGCCGGAGAAGCTGGCCGACGGTCGCCGGGTTCGCGTCACCGTCGATGTGTTCTGCAAGGGCACCTTCCGCGG	Size	Hit Count	Total Norm	Total
No Reads

dp5

TATGATGAGCCCGGAAATCGAGCAGTTCAGCAACTCAGTGCCAAAGTCCCCTATACGGGAACTGCTCGAACTGGAGCCGGAGACGGCCAAATTTGGGAAGCCGGAGAAGCTGGCCGACGGACGCCGGGTGCGCGTCACCGTCGATGTCTTCTGCAAGGGCACCTTCCGTGG	Size	Hit Count	Total Norm	Total	V112 Male-body	V043 Embryo	V051 Head	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head
..............................................................................................................................................GATGTCTTCTGCAAGGGCACC........	21	1	1.00	1	1	0	0	0	0	0	0

droPer2

TATGATGAGCCCGGAAATCGAGCAGTTCAGCAACTCAGTGCCAAAGTCCCCCATACGGGAACTGCTCGAACTGGAGCCGGAGACGGCCAAATTTGGGAAGCCGGAGAAGCTGGCCGACGGACGCCGGGTGCGCGTCACCGTCGATGTCTTCTGCAAGGGCACCTTCCGTGG	Size	Hit Count	Total Norm	Total	V111 Male-body	V042 Embryo	V050 Head	V057 Head	M021 Embryo	M042 Female-body
.......................................................................................................................................CACCGTCGATGTCTTCTGCAAG..............	22	1	1.00	1	0	0	0	0	1	0
.............................................................................................................................................CGATGTCTTCTGCAAGGGC...........	19	1	1.00	1	0	0	0	0	1	0

droWil2

CATGATGAAGCCTGAAATCGAACAATTCAGCAACTCAGTGCCAAAATCACCCATACGGGAACTTCTCGAACTGGAGCCCGAGACGGCCAAGTTTGGTAAACCCGAGAAATTGGCCGATGGTCGGCGGGTCCGTGTCACAGTCGATGTCTTCTGCAAGGGCACTTTCCGTGG	Size	Hit Count	Total Norm	Total	V117 Male-body	V118 Embryo	V119 Head	M020 Head	M045 Female-body
CATGATGAAGCCTGAAATCGAAC....................................................................................................................................................	23	1	1.00	1	0	1	0	0	0
......................................................................................................CGAGAAATTGGCCGATGGT..................................................	19	1	1.00	1	0	1	0	0	0
.................................................................................................................................................GTCTTCTGCAAGGGCACTTTCCGTG.	25	1	1.00	1	0	1	0	0	0
..................................................................................................................CGATGGTCGGCGGGTCCGTGTC...................................	22	1	1.00	1	1	0	0	0	0

droVir3

TATGATGAAGCCCGAGATCGAGCAATTTAGTAATTCTGTGCCAAAATCACCCATACGAGAGCTGCTCGAGCTGGAACCGGAGACGGCCAAGTTTGGTAAGCCCGAGAAGTTGGCCGATGGTCGTCGTGTACGCGTCACTGTCGATGTCTTCTGCAAGGGCACATTCCGTGG	Size	Hit Count	Total Norm	Total	V116 Male-body	V053 Head	V047 Embryo	M027 Male-body	M028 Head	M047 Female-body	M061 Embryo	SRR060654 Ovary	SRR060655 Testis	SRR060656 Ovary	SRR060657 Testis	SRR060658 Ovary	SRR060659 Testis	SRR060660 Ovary	SRR060661 Embryo	SRR060662 Embryo	SRR060663 Embryo	SRR060664 Male-body	SRR060665 Female-body	SRR060666 Male-body	SRR060667 Female-body	SRR060668 Male-body	SRR060669 Female-body	SRR060670 Testis	SRR060671 Male-body	SRR060672 Female-body	SRR060673 Ovary	SRR060674 Ovary	SRR060675 Ovary	SRR060676 Ovary	SRR060677 Ovary	SRR060678 Testis	SRR060679 Testis	SRR060680 Testis	SRR060681 Testis	SRR060682 Embryo	SRR060683 Testis	SRR060684 Embryo	SRR060685 Embryo	SRR060686 Embryo	SRR060687 Embryo	SRR060688 Ovary	SRR060689 Testis
TATGATGAAGCCCGAGATCG.......................................................................................................................................................	20	1	2.00	2	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.................................................................................................................CCGATGGTCGTCGTGTACGC......................................	20	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
....................................................................AGCTGGAACCGGAGACGGCCAAG................................................................................	23	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
..............................................................................................................TGGCCGATGGTCGTCGTGTACGCGT....................................	25	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.ATGATGAAGCCCGAGATCGAGC....................................................................................................................................................	22	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0
........................................................................................................................................ACTGTCGATGTCTTCTGCAAGGG............	23	1	1.00	1	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
............................................................GCTGCTCGAGCTGGAACCGGA..........................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..............................................................TGCTCGAGCTGGAACCGGAGAC.......................................................................................	22	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
................................................................................................................GCCGATGGTCGTCGTGTACGCGT....................................	23	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................................................................................................GAAGTTGGCCGATGGTCGTC..............................................	20	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..............................................................................................................TGGCCGATGGTCGTCGTGTACGC......................................	23	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
.................TCGAGCAATTTAGTAATTCT......................................................................................................................................	20	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

droMoj3

TATGATGAAGCCCGAGATAGAGCAGTTTAGCAATTCTGTGCCAAAGTCACCCATACGAGAGCTGCTCGAGCTGGAGCCGGAAACGGCCAAATTTGGCAAGCCAGAGAAACTGGCGGACGGACGTCGCGTTCGCGTCACTGTTGATGTCTTTTGCAAGGGCACGTTCCGCGG	Size	Hit Count	Total Norm	Total	V110 Male-body	V056 Head	V049 Head	V041 Embryo	M046 Female-body	M060 Embryo
No Reads

droGri2

CATGATGAAGCCGGAGATAGAACAATTTAGCAATTCTGTGCCAAAGTCACCCATTCGCGAGCTGCTCGAGCTCGAACCAGAAACGGCCAAGTTCGGCAAGCCAGAGAAGCTGGCAGACGGTCGCCGCGTACGCGTCACTGTGGATGTCTTCTGCAAGGGCACGTTCCGTGG	Size	Hit Count	Total Norm	Total	V109 Male-body	M041 Female-body
No Reads

dsi_109

3R:18370865-18370935 -

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-49.2	-49.1	-48.6