dsi-mir-975

AACGTATTTAGCACACGAGTACTGAAAAATTAACAACGAAACGGTTGAACTCGGAAGTGCAATATTGAGTTTTTGATTTTAAACACTGCCTACATCCTGTATGTGTTTTGCATCCGATACAGTTGTAGGAGTCGTTTGCAGTCAGAGATTTCACAGACTGCGAGGATCGAAGGAAGCACTGGAACCTCCGCATGCAGTGTCGTGGCATTGGTGAGGACAT **************************************************************..((((((((((((.((((((((.((((((..((((((((((...))))...)))))).))))))))).))))).))))))))))))...****************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	O001 Testis	O002 Head	M024 male body	M023 head	M025 embryo	SRR553485 Ovary	SRR553486 Ovary	SRR553487 Ovary	SRR553488 Ovary	SRR618934 Ovary
...............................................................................TAAACACTGCCTACATCCTGTAT......................................................................................................................	23	0	1	1752.00	1752	1706	32	12	1	0	0	0	0	1	0
...............................................................................TAAACACTGCCTACATCC...........................................................................................................................	18	0	1	834.00	834	822	12	0	0	0	0	0	0	0	0
...............................................................................TAAACACTGCCTACATCCTG.........................................................................................................................	20	0	1	168.00	168	161	6	1	0	0	0	0	0	0	0
...............................................................................TAAACACTGCCTACATCCT..........................................................................................................................	19	0	1	160.00	160	153	3	2	0	2	0	0	0	0	0
...............................................................................TAAACACTGCCTACATCCTGTA.......................................................................................................................	22	0	1	57.00	57	56	0	1	0	0	0	0	0	0	0
...............................................................................TAAACACTGCCTACATCCTGT........................................................................................................................	21	0	1	49.00	49	48	0	1	0	0	0	0	0	0	0
................................................................................AAACACTGCCTACATCCTGTAT......................................................................................................................	22	0	1	17.00	17	17	0	0	0	0	0	0	0	0	0
...............................................................................TAAACACTGCCTACATCCTGTATG.....................................................................................................................	24	0	1	11.00	11	11	0	0	0	0	0	0	0	0	0
.....................................................................................................................TACAGTTGTAGGAGTCGTTTGC.................................................................................	22	0	1	9.00	9	2	0	5	1	0	0	0	0	0	1
................................................................................AAACACTGCCTACATCCTG.........................................................................................................................	19	0	1	7.00	7	7	0	0	0	0	0	0	0	0	0
.....................................................................................................................TACAGTTGTAGGAGTCGTTTGCA................................................................................	23	0	1	5.00	5	1	0	3	0	0	0	1	0	0	0
...............................................................................TAAACACTGCCTACATCCTGTATGT....................................................................................................................	25	0	1	4.00	4	4	0	0	0	0	0	0	0	0	0
..............................................................................TTAAACACTGCCTACATCCTG.........................................................................................................................	21	0	1	3.00	3	2	0	1	0	0	0	0	0	0	0
................................................................................AAACACTGCCTACATCCT..........................................................................................................................	18	0	1	3.00	3	3	0	0	0	0	0	0	0	0	0
..............................................................................TTAAACACTGCCTACATCC...........................................................................................................................	19	0	1	3.00	3	3	0	0	0	0	0	0	0	0	0
......................................................................................................................ACAGTTGTAGGAGTCGTTTGCA................................................................................	22	0	1	2.00	2	1	0	1	0	0	0	0	0	0	0
.....................................................................................................................TACAGTTGTAGGAGTCGT.....................................................................................	18	0	1	2.00	2	2	0	0	0	0	0	0	0	0	0
......................................................................................................................ACAGTTGTAGGAGTCGTTTGC.................................................................................	21	0	1	2.00	2	0	0	2	0	0	0	0	0	0	0
..............................................................................TTAAACACTGCCTACATCCTGTAT......................................................................................................................	24	0	1	2.00	2	2	0	0	0	0	0	0	0	0	0
................................................................................AAACACTGCCTACATCCTGTA.......................................................................................................................	21	0	1	2.00	2	2	0	0	0	0	0	0	0	0	0
..............................................................................TTAAACACTGCCTACATCCT..........................................................................................................................	20	0	1	2.00	2	2	0	0	0	0	0	0	0	0	0
.....................................................................................................................TACAGTTGTAGGAGTCGTTT...................................................................................	20	0	1	2.00	2	1	0	0	0	0	1	0	0	0	0
...............................................................................TAAACACTGCCTACATCCTGTAC......................................................................................................................	23	1	1	2.00	2	0	0	2	0	0	0	0	0	0	0
..............................................................................TTAAACACTGCCTACATCCTGT........................................................................................................................	22	0	1	2.00	2	1	1	0	0	0	0	0	0	0	0
................................................................................AAACACTGCCTACATCCTGT........................................................................................................................	20	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0
..............................................................................TTAAACACTGCCTACATC............................................................................................................................	18	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0
.................................................................................AACACTGCCTACATCCTG.........................................................................................................................	18	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0
.....................................................................................................................TACAGTTGTAGGAGTCGTTTGCT................................................................................	23	1	1	1.00	1	0	0	1	0	0	0	0	0	0	0
...............................................................................CAAACACTGCCTACATCCTGTAT......................................................................................................................	23	1	1	1.00	1	0	0	0	0	0	1	0	0	0	0
....................................................................................ACTGCCTACATCCTGTATG.....................................................................................................................	19	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0
.................................................................................AACACTGCCTACATCCTGTAT......................................................................................................................	21	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0
.....................................................................................................................TACAGTTGTAGGAGTCGTTTG..................................................................................	21	0	1	1.00	1	0	0	1	0	0	0	0	0	0	0
............................................................................................................TGCATCCGATACAGTTGTAGGAGT........................................................................................	24	0	1	1.00	1	0	0	0	0	0	0	0	1	0	0
...............................................................................................................ATCAGATACCGTTGTAG............................................................................................	17	2	5	0.20	1	0	0	0	0	0	0	0	0	1	0
....................................CGGAACCGTAGAACTCGGAA....................................................................................................................................................................	20	3	7	0.14	1	0	0	1	0	0	0	0	0	0	0
.................................................................................................................................................................GAGGATCGAAGGCATCA..........................................	17	2	17	0.06	1	0	0	0	0	0	0	1	0	0	0
..............................................................................................................................................AAGAGATTTGACAGACT.............................................................	17	2	20	0.05	1	0	0	0	0	0	0	0	1	0	0

ATGTCCTCACCAATGCCACGACACTGCATGCGGAGGTTCCAGTGCTTCCTTCGATCCTCGCAGTCTGTGAAATCTCTGACTGCAAACGACTCCTACAACTGTATCGGATGCAAAACACATACAGGATGTAGGCAGTGTTTAAAATCAAAAACTCAATATTGCACTTCCGAGTTCAACCGTTTCGTTGTTAATTTTTCAGTACTCGTGTGCTAAATACGTT ****************************************************************...((((((((((((.(((((.(((((((((.((((((...((((...))))))))))..)))))).)))))))).))))))))))))..**************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total
................................................................................TGGAAACGACTCCTAATACTG.......................................................................................................................	21	3	1	1.00	1
.........................................................................................................................................................GAATATAGCACTTCCGAGGT...............................................	20	3	2	0.50	1
...........................ATGCGGAGGTTCCAGG.................................................................................................................................................................................	16	1	2	0.50	1
.............................................................................................................................AGGTAGGCAGTGGTTAAA.............................................................................	18	2	6	0.50	3
..........................CATGCGGAGGTTCGAATCCT..............................................................................................................................................................................	20	3	3	0.33	1
.........................................................................................................................................................................AGTTCCACAGTTTCGTTGCTA..............................	21	3	4	0.25	1
...........................ATGCGGAGGTTCGAATCCT..............................................................................................................................................................................	19	3	20	0.10	2
............................TGCGGAGGTTCCAGG.................................................................................................................................................................................	15	1	10	0.10	1
...........................................................................................................................TGATGTAGGTCGTGTTTAAAAT...........................................................................	22	3	13	0.08	1
.............................................TTCCTGCGGTCCTCGCA..............................................................................................................................................................	17	2	15	0.07	1
............................................TTTCCTTCGCTCCTCGCA..............................................................................................................................................................	18	2	17	0.06	1
..........................CATGCGGAGGTTCGAATCC...............................................................................................................................................................................	19	3	18	0.06	1
.............................................TTCCTGCGTTCCTCGCA..............................................................................................................................................................	17	2	20	0.05	1

Species	Coordinate	ID	Alignment
droSim3	X:15018320-15018539 +	dsi-mir-975	AACGTATTTAGCACACGAGTACTGAAAAATTAACAACGAAA-CGGTTGAACTCGGAAGT--------------------------GCAATATTGAGTTTTTGATTTTAAACACTGCCTACATCCTGTATGTGTTTTGC----ATCCGATAC-AGTTGTAGGAGTCGTTT-GCAGTCAGAGATTTCACAGACTGCGAGG-ATCGAAG----------------GAAGCACTGGAAC----------------------------------CTCC-G---CATGCAGTGTCGTGGCATTGGTGAGGACAT
droSec2	scaffold_8:1735062-1735273 +	dse_168	AACATATTTAGCACACGAGTACCGAAAAATTAACAAC--------------------------ATCAACAAATTAAAATT---AATTAATATTGAGTTTTTGATTTTAAACACTCCCTACATCCTGTATGTGTTTTGC----ATCCGATAC-AGTTGTAGGAGTCGTTT-GCAGTCAGAGATTTCACAGACTGCGAGG-ATCGCAG----------------GAAGCAC----------------------------------------CTCC-G---CATGCAGTGCCGTGGCATTGGTGAGGACAT
dm3	chrX:19452697-19452963 +	dme-mir-4966-2	AACATATTTAGCACTCAAGTACCGAAAAATGAACAACTAAAAGCGGAGAATACGAAAGTGCGGACCACCAAGTTAT--CCAATTGGCAATATTGAATTTTTGATTTTAAACACTTCCTACATCCTGTATGTGTTTTGC----ATCCGGTAC-AGATGTGGGAGTCGTTT-GCACTCAGAGATTTCACAGAATTCGAGG-ATCGACG----------------GATGCACTGGAATGTCTGCAACGGCGG----------TGGTGGGC--ATCG-C---CATGCAGTGCCGCGGCATTGGTGAGGCCAT
droEre2	scaffold_4690:9688699-9688942 +	der_56	----------GCACTCAAGTACCAAAAAGT-ACCAACGCAA-CCGGACAACCAGGGACTACGTCCAACC-----AT--CCAACCATCAACATTGAATTCTTGACTTTAAACACTTCCTACATCCTGTATGTGTTTTGC----GCCCTATAC-AGATGTGGGGGTCGTTT-GCACTCAGGGATTCCACAGGCTGCAAGG-ATCGGAG----------------GAAGCCTTGAAGCTCC------AGAGGTGT---------GCCAGAA---TC-G---CATGCAGTGCCTCGACATTGGTGAGGGCAT
droYak3	X:11885873-11886066 -	dya_50	G-------------------------------------------------------------------------------------CAATATTGAATTCTTGACTTTAAACACTGCCTACATACTGTATGTGTTTTGC----ACCCGATAC-AGATGTGGGGGTCGTTT-GCAATCAGAGATTCCGCAGGCTACGAGG-ATCGGGAGATCGGGAGTTTGGAGGAGGCATTGAAGTTCC------AGAGGTGA---------GTGAGAA---TC-G---CATGCAGTGCCACGACATTGGTGAGGGCAT
droEug1	scf7180000409230:879734-879890 -		------------------------------------------------------------------------------------------ATTGAATACTTGAATTTAAACACTGCCTACATCCCGTATGTGATATGA----TCCCGATAC-GGATGTGGGGATCGTTT-ACATTTAGAGATTCCGGAAACTTCGAATAATCGAGG----------------AAAGCATCGAAAC-----------------------------------ATC-T---GATGCAGCGCCTTGACATTGGCGAGGCCAA
droBia1	scf7180000302069:1151092-1151272 -		CAGA------------------------------------------------------------------------------AAGTCAATGGTGAGTTTCTGAATTTAAACACTGCCTACATCCCGTATGTGTCCTAA----TCCCGATAC-GGATGTGGGGGTCGTTT-GCACTCAGAGATTCCGGAGACTTCGAAA-GCGTGAGGGGGGGGA---------------------AA---------------TCAATGCTGGTGGGG--ATGA-G---GAAGCACCGCCTCGGCATTGGTGAGGGCAC
droTak1	scf7180000415169:844894-845069 -		--------------------------------------------------------------------------------------CGACATTGAATTCTTGAATTTAAACACTGCCTACATCCAGTATGTGATTTCA----TCCCGATAC-GGATGTGGGGGTCGTTT-GCACTCAGAGATTCCGGAGACTTCGAAGAATCTATT----------------CTAAAGCTGAATCTGG------AGAGGAGG------------AGA--AAGA-G---GAAGCATCGCCTCGACATTATTGAGGGCAC
droRho1	scf7180000761121:190177-190340 -		--------------------------------------------------------------------------------------------TGAATTCTTGAATTTAAACACTGCCTACATCCAGTATGTAAACTGA----TTCCGATAC-GGATGTGGGGGTTGTTT-GCACTCAGAGATTCCGGAGTCCTCGAAC-GTTGGAGGACCAGGACCTCGG--G-------------------------------------------AACCGCCTGCAAACAGCATCACCTCGCCATTGGTGAGGGCAT
droFic1	scf7180000454073:1733780-1733942 +		--------------------------------------------------------------------------------------------TGAATTCTTGAATCTAAACACTGCCTACATCCAGTATGTTTTTCGA----TTGCAATAC-GGATGTGGGGGTCGTTT-GCACTCAGAGATTCCGGAGTCTTCAGCC-GGCGAAGGACATCGAAGGAAT--G-------------------------------------------AATCACC-GCAGACAGCGGCGCCCCGCCATTAATAAGGACCT
droKik1	scf7180000302517:1183602-1183711 +		-------------------------------------------------------------------------------------------------------TCCTGAACACTGGCCATATTTAGTATGTGATTTGA----ACTTGATAC-GAATGTGATTGGCGTTTGGCACTTGGAGATTGCAG---------------------------------------------------------------------------------------C---GAGGCATTGCCTCGCCATTGGTGAGAGCAT
droAna3	scaffold_13417:2467634-2467801 -	dan_128	--------------------------------------------------------------------------------------CGACAATGAATATTTGATTTTAAACACTACCTACATCCAGTATGTTACTTGA---CTCCCAATAC-GGATGTGGAGGGCGTTT-ACACTCAGAGATTCTGGAGCCACTGCCG-TCCAGAGGGTCGAGC-------------------------------------------GCTACCGATG--CTCCCC---GAAGGAGCGCCTCGACATTGGTGAGTTCAT
droBip1	scf7180000395874:39999-40175 -		T-----------------------------------------------------------------------------CGAAAAGTCGACAATGAATACTTGATTTTAAACACTACCTACATCCAGTATGTTACTTGA---CTCCCAATAC-GGATGTGGAGGGCGTTT-ACACTCAGAGATTCTAGAGCCACTTCCG-TCCAGAGGGTCGAGC-------------------------------------------GCTAGCGATG--CTCCGT---GAAGGAGCGCCTCGCCATTGGTGGGGGCGT
droMoj3	scaffold_6328:2901044-2901225 -	dmo_205	-----------------------------------------------------------------------------------------------GTCTTTGATTGTAGATACTCCCTATATCCCGTATGTGCGATACTTTGATAAAATACAAGATGTATGGGGTGTCT-GCAATCACAAACCACATAGCCGCAATCA-AACAACTGGCCCAA--------AGTGTTATCGTTATCG---------------TTATCGCTAGCGACG--CTTC-G---GGTGCAGCCCCTCGCGATTGATGAAAGCAT

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droSim3

AACGTATTTAGCACACGAGTACTGAAAAATTAACAACGAAA-CGGTTGAACTCGGAAGT--------------------------GCAATATTGAGTTTTTGATTTTAAACACTGCCTACATCCTGTATGTGTTTTGC----ATCCGATAC-AGTTGTAGGAGTCGTTT-GCAGTCAGAGATTTCACAGACTGCGAGG-ATCGAAG----------------GAAGCACTGGAAC----------------------------------CTCC-G---CATGCAGTGTCGTGGCATTGGTGAGGACAT	Size	Hit Count	Total Norm	Total	V044 Embryo	M023 Head	M024 Male-body	M025 Embryo	O001 Testis	O002 Head	M053 Female-body	SRR553485 Ovary	SRR553486 Ovary	SRR553487 Ovary	SRR553488 Ovary	SRR618934 Ovary
..........................................................................................................TAAACACTGCCTACATCCTGTAT...................................................................................................................................................................................	23	1	1752.00	1752	0	1	12	0	1706	32	0	0	0	0	1	0
..........................................................................................................TAAACACTGCCTACATCC........................................................................................................................................................................................	18	1	834.00	834	0	0	0	0	822	12	0	0	0	0	0	0
..........................................................................................................TAAACACTGCCTACATCCTG......................................................................................................................................................................................	20	1	168.00	168	0	0	1	0	161	6	0	0	0	0	0	0
..........................................................................................................TAAACACTGCCTACATCCT.......................................................................................................................................................................................	19	1	160.00	160	0	0	2	2	153	3	0	0	0	0	0	0
..........................................................................................................TAAACACTGCCTACATCCTGTA....................................................................................................................................................................................	22	1	57.00	57	0	0	1	0	56	0	0	0	0	0	0	0
..........................................................................................................TAAACACTGCCTACATCCTGT.....................................................................................................................................................................................	21	1	49.00	49	0	0	1	0	48	0	0	0	0	0	0	0
...........................................................................................................AAACACTGCCTACATCCTGTAT...................................................................................................................................................................................	22	1	17.00	17	0	0	0	0	17	0	0	0	0	0	0	0
..........................................................................................................TAAACACTGCCTACATCCTGTATG..................................................................................................................................................................................	24	1	11.00	11	0	0	0	0	11	0	0	0	0	0	0	0
....................................................................................................................................................TAC-AGTTGTAGGAGTCGTTT-GC........................................................................................................................................	22	1	9.00	9	0	1	5	0	2	0	0	0	0	0	0	1
...........................................................................................................AAACACTGCCTACATCCTG......................................................................................................................................................................................	19	1	7.00	7	0	0	0	0	7	0	0	0	0	0	0	0
....................................................................................................................................................TAC-AGTTGTAGGAGTCGTTT-GCA.......................................................................................................................................	23	1	5.00	5	0	0	3	0	1	0	0	0	1	0	0	0
..........................................................................................................TAAACACTGCCTACATCCTGTATGT.................................................................................................................................................................................	25	1	4.00	4	0	0	0	0	4	0	0	0	0	0	0	0
.........................................................................................................TTAAACACTGCCTACATCCTG......................................................................................................................................................................................	21	1	3.00	3	0	0	1	0	2	0	0	0	0	0	0	0
...........................................................................................................AAACACTGCCTACATCCT.......................................................................................................................................................................................	18	1	3.00	3	0	0	0	0	3	0	0	0	0	0	0	0
.........................................................................................................TTAAACACTGCCTACATCC........................................................................................................................................................................................	19	1	3.00	3	0	0	0	0	3	0	0	0	0	0	0	0
.....................................................................................................................................................AC-AGTTGTAGGAGTCGTTT-GCA.......................................................................................................................................	22	1	2.00	2	0	0	1	0	1	0	0	0	0	0	0	0
....................................................................................................................................................TAC-AGTTGTAGGAGTCGT.............................................................................................................................................	18	1	2.00	2	0	0	0	0	2	0	0	0	0	0	0	0
.....................................................................................................................................................AC-AGTTGTAGGAGTCGTTT-GC........................................................................................................................................	21	1	2.00	2	0	0	2	0	0	0	0	0	0	0	0	0
.........................................................................................................TTAAACACTGCCTACATCCTGTAT...................................................................................................................................................................................	24	1	2.00	2	0	0	0	0	2	0	0	0	0	0	0	0
...........................................................................................................AAACACTGCCTACATCCTGTA....................................................................................................................................................................................	21	1	2.00	2	0	0	0	0	2	0	0	0	0	0	0	0
.........................................................................................................TTAAACACTGCCTACATCCT.......................................................................................................................................................................................	20	1	2.00	2	0	0	0	0	2	0	0	0	0	0	0	0
....................................................................................................................................................TAC-AGTTGTAGGAGTCGTTT-..........................................................................................................................................	20	1	2.00	2	0	0	0	0	1	0	0	1	0	0	0	0
.........................................................................................................TTAAACACTGCCTACATCCTGT.....................................................................................................................................................................................	22	1	2.00	2	0	0	0	0	1	1	0	0	0	0	0	0
...........................................................................................................AAACACTGCCTACATCCTGT.....................................................................................................................................................................................	20	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
.........................................................................................................TTAAACACTGCCTACATC.........................................................................................................................................................................................	18	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
............................................................................................................AACACTGCCTACATCCTG......................................................................................................................................................................................	18	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
...............................................................................................................ACTGCCTACATCCTGTATG..................................................................................................................................................................................	19	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
............................................................................................................AACACTGCCTACATCCTGTAT...................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
....................................................................................................................................................TAC-AGTTGTAGGAGTCGTTT-G.........................................................................................................................................	21	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0
.......................................................................................................................................TGC----ATCCGATAC-AGTTGTAGGAGT................................................................................................................................................	24	1	1.00	1	0	0	0	0	0	0	0	0	0	1	0	0

droSec2

AACATATTTAGCACACGAGTACCGAAAAATTAACAAC--------------------------ATCAACAAATTAAAATT---AATTAATATTGAGTTTTTGATTTTAAACACTCCCTACATCCTGTATGTGTTTTGC----ATCCGATAC-AGTTGTAGGAGTCGTTT-GCAGTCAGAGATTTCACAGACTGCGAGG-ATCGCAG----------------GAAGCAC----------------------------------------CTCC-G---CATGCAGTGCCGTGGCATTGGTGAGGACAT	Size	Hit Count	Total Norm	Total	V113 Male-body	V114 Embryo	V115 Head	M054 Female-body
.........................................................................................................TTAAACACTCCCTACATCCTGT.....................................................................................................................................................................................	22	1	5.00	5	3	0	2	0
..........................................................................................................TAAACACTCCCTACATCCTGTAT...................................................................................................................................................................................	23	1	5.00	5	2	0	0	3
....................................................................................................................................................TAC-AGTTGTAGGAGTCGTTT-GCA.......................................................................................................................................	23	1	3.00	3	3	0	0	0
.....................................................................................................................................................AC-AGTTGTAGGAGTCGTTT-GCA.......................................................................................................................................	22	1	3.00	3	3	0	0	0
....................................................................................................................................................................................................................................C----------------------------------------CTCC-G---CATGCAGTGCCGTGG...............	21	1	2.00	2	1	0	1	0
.........................................................................................................TTAAACACTCCCTACATCCTGTA....................................................................................................................................................................................	23	1	2.00	2	0	0	0	2
......................................................................................................................................................C-AGTTGTAGGAGTCGTTT-GCAG......................................................................................................................................	22	1	2.00	2	1	0	1	0
..........................................................................................................TAAACACTCCCTACATCCTGT.....................................................................................................................................................................................	21	1	1.00	1	1	0	0	0
....................................................................................................................................................TAC-AGTTGTAGGAGTCGTTT-..........................................................................................................................................	20	1	1.00	1	1	0	0	0
..................................................................................................................................................................................................................................CAC----------------------------------------CTCC-G---CATGCAGTGCCGTGG...............	23	1	1.00	1	1	0	0	0
......................................................................................................................................................C-AGTTGTAGGAGTCGTTT-GCA.......................................................................................................................................	21	1	1.00	1	1	0	0	0
...................................................................................................................................................................................................................................AC----------------------------------------CTCC-G---CATGCAGTGCCGTGG...............	22	1	1.00	1	1	0	0	0
.............................................................................................................................................................................................................................................................................CTCC-G---CATGCAGTGCCGTGG...............	20	1	1.00	1	1	0	0	0
....................................................................................................................................................TAC-AGTTGTAGGAGTCGTTT-GC........................................................................................................................................	22	1	1.00	1	1	0	0	0

droEre2

----------GCACTCAAGTACCAAAAAGT-ACCAACGCAA-CCGGACAACCAGGGACTACGTCCAACC-----AT--CCAACCATCAACATTGAATTCTTGACTTTAAACACTTCCTACATCCTGTATGTGTTTTGC----GCCCTATAC-AGATGTGGGGGTCGTTT-GCACTCAGGGATTCCACAGGCTGCAAGG-ATCGGAG----------------GAAGCCTTGAAGCTCC------AGAGGTGT---------GCCAGAA---TC-G---CATGCAGTGCCTCGACATTGGTGAGGGCAT	Size	Hit Count	Total Norm	Total	V040 Embryo	V060 Head	V107 Male-body	V108 Head	M055 Female-body	M057 Embryo
..........................................................................................................TAAACACTTCCTACATCCTGTAT...................................................................................................................................................................................	23	1	10.00	10	0	0	4	0	6	0
.........................................................................................................TTAAACACTTCCTACATCCTGT.....................................................................................................................................................................................	22	1	10.00	10	0	0	4	6	0	0
.........................................................................................................TTAAACACTTCCTACATC.........................................................................................................................................................................................	18	1	3.00	3	0	0	2	1	0	0
.........................................................................................................TTAAACACTTCCTACATCCTGTA....................................................................................................................................................................................	23	1	3.00	3	0	0	0	1	2	0
.......................................................................................AACATTGAATTCTTGACTT..........................................................................................................................................................................................................	19	1	1.00	1	0	0	0	1	0	0
..........................................................................................................TAAACACTTCCTACATCCTGT.....................................................................................................................................................................................	21	1	1.00	1	0	0	1	0	0	0
.....................................................................................................................................................AC-AGATGTGGGGGTCGTTT-GC........................................................................................................................................	21	1	1.00	1	0	0	1	0	0	0
...........................................................................................................................................................................................................................................................................A---TC-G---CATGCAGTGCCTCGAC..............	20	1	1.00	1	0	0	1	0	0	0
...........................................................................................................AAACACTTCCTACATCCTGTAT...................................................................................................................................................................................	22	1	1.00	1	0	0	0	0	1	0
.....................................................................................................................................................AC-AGATGTGGGGGTCGTTT-G.........................................................................................................................................	20	1	1.00	1	0	0	1	0	0	0

droYak3

G-------------------------------------------------------------------------------------CAATATTGAATTCTTGACTTTAAACACTGCCTACATACTGTATGTGTTTTGC----ACCCGATAC-AGATGTGGGGGTCGTTT-GCAATCAGAGATTCCGCAGGCTACGAGG-ATCGGGAGATCGGGAGTTTGGAGGAGGCATTGAAGTTCC------AGAGGTGA---------GTGAGAA---TC-G---CATGCAGTGCCACGACATTGGTGAGGGCAT	Size	Hit Count	Total Norm	Total	V046 Embryo	V052 Head	V120 Male-body	V058 Head	M026 Head	M043 Female-body	M056 Embryo
.........................................................................................................TTAAACACTGCCTACATACTG......................................................................................................................................................................................	21	1	121.00	121	0	0	1	0	118	2	0
.........................................................................................................TTAAACACTGCCTACATACTGT.....................................................................................................................................................................................	22	1	23.00	23	0	0	0	0	22	1	0
.........................................................................................................TTAAACACTGCCTACATACTGTA....................................................................................................................................................................................	23	1	22.00	22	0	0	1	0	20	1	0
..........................................................................................................TAAACACTGCCTACATACTGTAT...................................................................................................................................................................................	23	1	11.00	11	0	1	3	0	3	4	0
..........................................................................................................TAAACACTGCCTACATACTGTA....................................................................................................................................................................................	22	1	5.00	5	0	0	2	0	1	2	0
..........................................................................................................TAAACACTGCCTACATACTG......................................................................................................................................................................................	20	1	3.00	3	0	0	0	0	3	0	0
...............................................................................................................................................................................................................................................................................TC-G---CATGCAGTGCCACGAC..............	19	1	2.00	2	0	0	0	0	2	0	0
.....................................................................................................................................................AC-AGATGTGGGGGTCGTTT-GC........................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	1
.....................................................................................................................................................AC-AGATGTGGGGGTCGTTT-G.........................................................................................................................................	20	1	1.00	1	0	0	1	0	0	0	0
......................................................................................................................................................C-AGATGTGGGGGTCGTTT-GCA.......................................................................................................................................	21	1	1.00	1	0	0	1	0	0	0	0
.....................................................................................................................................................AC-AGATGTGGGGGTCGTTT-GCA.......................................................................................................................................	22	1	1.00	1	0	0	1	0	0	0	0
.........................................................................................................TTAAACACTGCCTACATAC........................................................................................................................................................................................	19	1	1.00	1	0	0	0	0	1	0	0
......................................................................................................................................................C-AGATGTGGGGGTCGTTT-G.........................................................................................................................................	19	1	1.00	1	0	0	1	0	0	0	0

droEug1

------------------------------------------------------------------------------------------ATTGAATACTTGAATTTAAACACTGCCTACATCCCGTATGTGATATGA----TCCCGATAC-GGATGTGGGGATCGTTT-ACATTTAGAGATTCCGGAAACTTCGAATAATCGAGG----------------AAAGCATCGAAAC-----------------------------------ATC-T---GATGCAGCGCCTTGACATTGGCGAGGCCAA	Size	Hit Count	Total Norm	Total
No Reads

droBia1

CAGA------------------------------------------------------------------------------AAGTCAATGGTGAGTTTCTGAATTTAAACACTGCCTACATCCCGTATGTGTCCTAA----TCCCGATAC-GGATGTGGGGGTCGTTT-GCACTCAGAGATTCCGGAGACTTCGAAA-GCGTGAGGGGGGGGA---------------------AA---------------TCAATGCTGGTGGGG--ATGA-G---GAAGCACCGCCTCGGCATTGGTGAGGGCAC	Size	Hit Count	Total Norm	Total
No Reads

droTak1

--------------------------------------------------------------------------------------CGACATTGAATTCTTGAATTTAAACACTGCCTACATCCAGTATGTGATTTCA----TCCCGATAC-GGATGTGGGGGTCGTTT-GCACTCAGAGATTCCGGAGACTTCGAAGAATCTATT----------------CTAAAGCTGAATCTGG------AGAGGAGG------------AGA--AAGA-G---GAAGCATCGCCTCGACATTATTGAGGGCAC	Size	Hit Count	Total Norm	Total
No Reads

droRho1

--------------------------------------------------------------------------------------------TGAATTCTTGAATTTAAACACTGCCTACATCCAGTATGTAAACTGA----TTCCGATAC-GGATGTGGGGGTTGTTT-GCACTCAGAGATTCCGGAGTCCTCGAAC-GTTGGAGGACCAGGACCTCGG--G-------------------------------------------AACCGCCTGCAAACAGCATCACCTCGCCATTGGTGAGGGCAT	Size	Hit Count	Total Norm	Total
No Reads

droFic1

--------------------------------------------------------------------------------------------TGAATTCTTGAATCTAAACACTGCCTACATCCAGTATGTTTTTCGA----TTGCAATAC-GGATGTGGGGGTCGTTT-GCACTCAGAGATTCCGGAGTCTTCAGCC-GGCGAAGGACATCGAAGGAAT--G-------------------------------------------AATCACC-GCAGACAGCGGCGCCCCGCCATTAATAAGGACCT	Size	Hit Count	Total Norm	Total
No Reads

droKik1

-------------------------------------------------------------------------------------------------------TCCTGAACACTGGCCATATTTAGTATGTGATTTGA----ACTTGATAC-GAATGTGATTGGCGTTTGGCACTTGGAGATTGCAG---------------------------------------------------------------------------------------C---GAGGCATTGCCTCGCCATTGGTGAGAGCAT	Size	Hit Count	Total Norm	Total
No Reads

droAna3

--------------------------------------------------------------------------------------CGACAATGAATATTTGATTTTAAACACTACCTACATCCAGTATGTTACTTGA---CTCCCAATAC-GGATGTGGAGGGCGTTT-ACACTCAGAGATTCTGGAGCCACTGCCG-TCCAGAGGGTCGAGC-------------------------------------------GCTACCGATG--CTCCCC---GAAGGAGCGCCTCGACATTGGTGAGTTCAT	Size	Hit Count	Total Norm	Total	V105 Male-body	V106 Head	V055 Head	V039 Embryo	M044 Female-body	M058 Embryo
.....................................................................................................................................................AC-GGATGTGGAGGGCGTTT-ACA.......................................................................................................................................	22	1	52.00	52	22	4	0	0	26	0
.....................................................................................................................................................AC-GGATGTGGAGGGCGTTT-..........................................................................................................................................	19	1	35.00	35	16	0	2	0	17	0
.....................................................................................................................................................AC-GGATGTGGAGGGCGTTT-AC........................................................................................................................................	21	1	32.00	32	17	3	5	0	7	0
..........................................................................................................TAAACACTACCTACATCCAGTAT...................................................................................................................................................................................	23	1	18.00	18	2	1	1	0	13	1
.....................................................................................................................................................AC-GGATGTGGAGGGCGTTT-A.........................................................................................................................................	20	1	7.00	7	3	0	1	0	3	0
................................................................................................................................................................................................................................................................................CCC---GAAGGAGCGCCTCGAC..............	19	1	2.00	2	0	0	0	0	2	0
.....................................................................................................................................................AC-GGATGTGGAGGGCGTT............................................................................................................................................	18	1	2.00	2	1	0	1	0	0	0
......................................................................................................................................................C-GGATGTGGAGGGCGTTT-ACA.......................................................................................................................................	21	1	2.00	2	0	0	0	0	2	0
..........................................................................................................TAAACACTACCTACATCCAGTA....................................................................................................................................................................................	22	1	1.00	1	0	0	0	0	1	0
......................................................................................................................................................C-GGATGTGGAGGGCGTTT-AC........................................................................................................................................	20	1	1.00	1	0	0	0	0	1	0
..............................................................................................................................................................................................................................................................................TCCCC---GAAGGAGCGCCTCGAC..............	21	1	1.00	1	0	0	0	0	1	0
....................................................................................................................................................TAC-GGATGTGGAGGGCGTTT-..........................................................................................................................................	20	1	1.00	1	1	0	0	0	0	0

droBip1

T-----------------------------------------------------------------------------CGAAAAGTCGACAATGAATACTTGATTTTAAACACTACCTACATCCAGTATGTTACTTGA---CTCCCAATAC-GGATGTGGAGGGCGTTT-ACACTCAGAGATTCTAGAGCCACTTCCG-TCCAGAGGGTCGAGC-------------------------------------------GCTAGCGATG--CTCCGT---GAAGGAGCGCCTCGCCATTGGTGGGGGCGT	Size	Hit Count	Total Norm	Total
No Reads

droMoj3

-----------------------------------------------------------------------------------------------GTCTTTGATTGTAGATACTCCCTATATCCCGTATGTGCGATACTTTGATAAAATACAAGATGTATGGGGTGTCT-GCAATCACAAACCACATAGCCGCAATCA-AACAACTGGCCCAA--------AGTGTTATCGTTATCG---------------TTATCGCTAGCGACG--CTTC-G---GGTGCAGCCCCTCGCGATTGATGAAAGCAT	Size	Hit Count	Total Norm	Total	V110 Male-body	V056 Head	V049 Head	V041 Embryo	M046 Female-body	M060 Embryo
.....................................................................................................................................................ACAAGATGTATGGGGTGTCT-GC........................................................................................................................................	22	1	1189.00	1189	1156	12	10	1	5	5
.....................................................................................................................................................ACAAGATGTATGGGGTGTCT-G.........................................................................................................................................	21	1	1137.00	1137	1117	4	6	0	10	0
.....................................................................................................................................................ACAAGATGTATGGGGTGTCT-..........................................................................................................................................	20	1	1014.00	1014	1004	4	3	0	3	0
......................................................................................................................................................CAAGATGTATGGGGTGTCT-GC........................................................................................................................................	21	1	126.00	126	120	1	0	0	5	0
.......................................................................................................................................................AAGATGTATGGGGTGTCT-GC........................................................................................................................................	20	1	61.00	61	59	0	1	0	1	0
.......................................................................................................................................................AAGATGTATGGGGTGTCT-G.........................................................................................................................................	19	1	37.00	37	37	0	0	0	0	0
......................................................................................................................................................CAAGATGTATGGGGTGTCT-G.........................................................................................................................................	20	1	25.00	25	20	1	0	0	4	0
.....................................................................................................................................................ACAAGATGTATGGGGTGTC............................................................................................................................................	19	1	20.00	20	19	0	0	0	1	0
..........................................................................................................TAGATACTCCCTATATCCCGT.....................................................................................................................................................................................	21	1	12.00	12	12	0	0	0	0	0
.......................................................................................................................................................AAGATGTATGGGGTGTCT-GCA.......................................................................................................................................	21	1	8.00	8	7	0	0	0	1	0
.......................................................................................................................................................AAGATGTATGGGGTGTCT-..........................................................................................................................................	18	1	8.00	8	8	0	0	0	0	0
..........................................................................................................TAGATACTCCCTATATCCCGTA....................................................................................................................................................................................	22	1	5.00	5	2	0	0	0	2	1
......................................................................................................................................................CAAGATGTATGGGGTGTCT-..........................................................................................................................................	19	1	5.00	5	4	0	0	0	1	0
.....................................................................................................................................................ACAAGATGTATGGGGTGT.............................................................................................................................................	18	1	4.00	4	4	0	0	0	0	0
........................................................................................................................................................AGATGTATGGGGTGTCT-GC........................................................................................................................................	19	1	2.00	2	2	0	0	0	0	0
.....................................................................................................................................................ACAAGATGTATGGGGTGTCT-GCA.......................................................................................................................................	23	1	2.00	2	2	0	0	0	0	0
.......................................................................................................................................................AAGATGTATGGGGTGTCT-GCAA......................................................................................................................................	22	1	2.00	2	2	0	0	0	0	0
...........................................................................................................AGATACTCCCTATATCCCGTA....................................................................................................................................................................................	21	1	1.00	1	1	0	0	0	0	0
........................................................................................................................................................AGATGTATGGGGTGTCT-GCAAT.....................................................................................................................................	22	1	1.00	1	0	0	0	0	1	0
......................................................................................................................................................CAAGATGTATGGGGTGTC............................................................................................................................................	18	1	1.00	1	1	0	0	0	0	0
........................................................................................................................................................AGATGTATGGGGTGTCT-G.........................................................................................................................................	18	1	1.00	1	1	0	0	0	0	0
....................................................................................................................................................TACAAGATGTATGGGGTGTCT-..........................................................................................................................................	21	1	1.00	1	1	0	0	0	0	0

dsi-mir-975

X:15018370-15018489 +

Known

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-30.9	-30.9	-30.9