ID:

dsi-mir-962

 Coordinate:

2L:5434384-5434477 +

 Confidence:

Known

 Type:

Unknown

 [View on miRBase]
[View on UCSC Genome Browser {Cornell Mirror}]
Legend: mature star mismatch in alignment mismatch in read

Predicted structure

-31.0 -30.9

Flybase annnotation

Antisense to intron [Dsim\GD22639-in]

No Repeatable elements found

Sense Strand Reads

GTTTTCTGGCAATCAAAGGAACTCGACTCGACTTGAATCTACAACTACCTCGATGGGGCGCTCAGGCTATAAGGTAGAGAAATTGATGTTGTCTACACTATTCAGACTTCAGTTTCATTGCCTTTCAATTTGTTTGCCCCCCTGCAACAGTGATAGTGATAACATCGTTGGACCAGACAATGCACTACAAAGGA
*****************************************************.((((((..((((....(((((((.((((((((.(((..............))).)))))))).)))))))....))))..))))))..**************************************************** 		Read size # Mismatch Hit Count Total Norm Total SRR618934
Ovary		 O001
Testis
 M024
male body
 SRR553486
Ovary		 SRR553485
Ovary		 M025
embryo
 M053
Female-body		 O002
Head
 GSM343915
embryo
 SRR553487
Ovary		 M023
head
....................................................................ATAAGGTAGAGAAATTGATGTTGTC..................................................................................................... 25 0 1 522.00 522 122 65 243 39 22 19 5 1 6 0 0
....................................................................ATAAGGTAGAGAAATTGATGTT........................................................................................................ 22 0 1 404.00 404 168 97 22 72 36 0 3 1 3 1 1
....................................................................ATAAGGTAGAGAAATTGATGTTGT...................................................................................................... 24 0 1 244.00 244 134 2 40 37 28 0 3 0 0 0 0
....................................................................ATAAGGTAGAGAAATTGATGTTGTCT.................................................................................................... 26 0 1 206.00 206 95 1 52 37 16 2 1 0 1 1 0
....................................................................ATAAGGTAGAGAAATTGATGTTG....................................................................................................... 23 0 1 148.00 148 57 64 2 13 10 0 1 1 0 0 0
....................................................................ATAAGGTAGAGAAATTGATG.......................................................................................................... 20 0 1 128.00 128 10 114 3 0 0 0 0 1 0 0 0
....................................................................ATAAGGTAGAGAAATTGA............................................................................................................ 18 0 1 97.00 97 2 95 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGTAGAGAAATTGAT........................................................................................................... 19 0 1 77.00 77 1 74 0 0 0 0 0 2 0 0 0
....................................................................ATAAGGTAGAGAAATTGATGT......................................................................................................... 21 0 1 75.00 75 17 47 2 3 3 0 0 3 0 0 0
.........................................................................................................ACTTCAGTTTCATTGCCTTTCA................................................................... 22 0 1 40.00 40 0 38 1 0 1 0 0 0 0 0 0
....................................................................ATAAGGTAGAGAAATT.............................................................................................................. 16 0 1 38.00 38 38 0 0 0 0 0 0 0 0 0 0
.........................................................................................................ACTTCAGTTTCATTGCCTTTC.................................................................... 21 0 1 18.00 18 0 18 0 0 0 0 0 0 0 0 0
.........................................................................................................ACTTCAGTTTCATTGCCT....................................................................... 18 0 1 17.00 17 0 16 0 0 0 0 0 1 0 0 0
..........................................................................................................CTTCAGTTTCATTGCCTTTCA................................................................... 21 0 1 8.00 8 0 4 3 1 0 0 0 0 0 0 0
....................................................................ATAAGGTAGAGAAAT............................................................................................................... 15 0 1 8.00 8 8 0 0 0 0 0 0 0 0 0 0
..........................................................................................................CTTCAGTTTCATTGCCTTTCAA.................................................................. 22 0 1 7.00 7 0 5 1 1 0 0 0 0 0 0 0
....................................................................ATAAGGTAGAGAAATTGATGTTGTCC.................................................................................................... 26 1 1 6.00 6 0 0 5 0 0 1 0 0 0 0 0
.........................................................................................................ACTTCAGTTTCATTGCCTT...................................................................... 19 0 1 5.00 5 0 5 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGTAGAGAAATTGATGCTGTC..................................................................................................... 25 1 1 5.00 5 3 0 0 1 0 0 0 0 0 1 0
..........................................................................................................CTTCAGTTTCATTGCCTT...................................................................... 18 0 1 5.00 5 0 5 0 0 0 0 0 0 0 0 0
.....................................................................TAAGGTAGAGAAATTGATGTTGTCT.................................................................................................... 25 0 1 4.00 4 2 0 2 0 0 0 0 0 0 0 0
....................................................................ATAAGGTAGAGAAATTGATGTTT....................................................................................................... 23 1 1 4.00 4 2 0 0 1 1 0 0 0 0 0 0
.........................................................................................................ACTTCAGTTTCATTGCCTTT..................................................................... 20 0 1 3.00 3 0 3 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGTAGAGAAATTGATGTTGC...................................................................................................... 24 1 1 3.00 3 1 0 2 0 0 0 0 0 0 0 0
....................................................................ATAATGTAGAGAAATTGATGTTGTC..................................................................................................... 25 1 1 2.00 2 0 0 1 1 0 0 0 0 0 0 0
....................................................................ATAAGGTAGAGAAATTGATATT........................................................................................................ 22 1 1 2.00 2 2 0 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGTAGAGAAATTG............................................................................................................. 17 0 1 2.00 2 2 0 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGTAGAGAAATTGATGTTGTCG.................................................................................................... 26 1 1 2.00 2 0 0 2 0 0 0 0 0 0 0 0
......................................................................AAGGTAGAGAAATTGATG.......................................................................................................... 18 0 1 2.00 2 0 2 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGTAGAAAAATTGATGTTGT...................................................................................................... 24 1 1 2.00 2 2 0 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGTAGAGAAATTGATATTGTC..................................................................................................... 25 1 1 2.00 2 2 0 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGGAGAGAAATTGATGTTGTC..................................................................................................... 25 1 1 2.00 2 0 0 2 0 0 0 0 0 0 0 0
.....................................................................TAAGGTAGAGAAATTGATGTTGT...................................................................................................... 23 0 1 2.00 2 1 0 0 1 0 0 0 0 0 0 0
.........................................................................................................ACTTCAGTTTCATTGCCTTTCAA.................................................................. 23 0 1 1.00 1 0 0 0 1 0 0 0 0 0 0 0
....................................................................ATCAGGTTGAGAAATTGATGTTGT...................................................................................................... 24 2 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
...........................................................................AGAGAAATTGATGTTGTC..................................................................................................... 18 0 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGTAGAGATATTGATGTTGTT..................................................................................................... 25 2 1 1.00 1 0 0 0 0 0 0 0 0 1 0 0
....................................................................ATAAGGTAGAGAAATTGATGTTGTT..................................................................................................... 25 1 1 1.00 1 0 0 1 0 0 0 0 0 0 0 0
....................................................................ATAAGGTAGAGAAATTGATTTTGT...................................................................................................... 24 1 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGAAGAGAAATTGATGTTGTC..................................................................................................... 25 1 1 1.00 1 0 0 1 0 0 0 0 0 0 0 0
....................................................................ATAAGGTAGAGAAATTGATGTCGTCT.................................................................................................... 26 1 1 1.00 1 0 0 1 0 0 0 0 0 0 0 0
....................................................................ATAAGGGAGAGAAATTGATGTTGTCT.................................................................................................... 26 1 1 1.00 1 0 0 1 0 0 0 0 0 0 0 0
....................................................................ACAAGGTAGAGAAATTGATGTTGT...................................................................................................... 24 1 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
..........................................................................TAGAGAAATTGATGTTGT...................................................................................................... 18 0 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGTAGGGAAATTGATGTTGT...................................................................................................... 24 1 1 1.00 1 0 0 1 0 0 0 0 0 0 0 0
....................................................................AGAAGGTAGAGAAATTGATGTTGT...................................................................................................... 24 1 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
......................................................................AAGGTAGAGAAATTGATGTTG....................................................................................................... 21 0 1 1.00 1 0 1 0 0 0 0 0 0 0 0 0
....................................................................ATAATGTAGAGAAATTGATGTT........................................................................................................ 22 1 1 1.00 1 0 0 0 1 0 0 0 0 0 0 0
....................................................................ATGAGGTAGAGAAATTGATGTTGTT..................................................................................................... 25 2 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGTAGAGTAATTGATGTT........................................................................................................ 22 1 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGTAGAGAAATTGATGTTC....................................................................................................... 23 1 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGTAGAAAAATTGATGTTGTCT.................................................................................................... 26 1 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGTAGAGAAATTGATGTGT....................................................................................................... 23 2 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGCAGAGAAATTGATGTTGTC..................................................................................................... 25 1 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGTAGAGAAATTGATTTGT....................................................................................................... 23 3 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGTAGAGAAATTGATGTTATCT.................................................................................................... 26 1 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
GTTTTCTGGCAATCAAAGG............................................................................................................................................................................... 19 0 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
..........................................................................TAGAGAAATTGATGTTGTC..................................................................................................... 19 0 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGTAGAGAGATTGATGTTGTCT.................................................................................................... 26 1 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
...........................................................................AGAGAAATTGATGTTGT...................................................................................................... 17 0 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
.....................................................................TAAGGTAGAGAAATTGATGTTGTCC.................................................................................................... 25 1 1 1.00 1 0 0 1 0 0 0 0 0 0 0 0
....................................................................GTAAGGTAGAGAAATTGATGTTGTCT.................................................................................................... 26 1 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
.....................................................................................................TCAGACTTCAGTTTCATTGCCT....................................................................... 22 0 1 1.00 1 0 0 0 0 0 1 0 0 0 0 0
..........................................................................................................CTTCAGTTTCATTGCCTTTC.................................................................... 20 0 1 1.00 1 0 1 0 0 0 0 0 0 0 0 0
....................................................................ATTAGGTAGAGAAATTGATGTTGTC..................................................................................................... 25 1 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
..TTTCTGGCAATCAAAGG............................................................................................................................................................................... 17 0 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGTAGAGAAGTTGATGTTGTCT.................................................................................................... 26 1 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGTAGGGAAATTGATGTTGCCT.................................................................................................... 26 2 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGTAGAGAAACTGATGTTGT...................................................................................................... 24 1 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
....................................................................ATAAAGTAGAGAAATTGATGTTGTC..................................................................................................... 25 1 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
....................................................................ATAACGTAGAGAAATTGATGTTGTC..................................................................................................... 25 1 1 1.00 1 0 0 1 0 0 0 0 0 0 0 0
....................................................................AGAAGGTAGAGAAATTGATGTTGTCT.................................................................................................... 26 1 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGAAGAGAAGTTGATGTTGTC..................................................................................................... 25 2 1 1.00 1 0 0 1 0 0 0 0 0 0 0 0
.....................................................................AAAGGTAGAGAAATTGATGTTGTCT.................................................................................................... 25 1 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGTAGAAAAATTGATGTT........................................................................................................ 22 1 1 1.00 1 0 0 0 1 0 0 0 0 0 0 0
....................................................................ATAATGTAGAGAAATTGATGTTGT...................................................................................................... 24 1 1 1.00 1 0 0 1 0 0 0 0 0 0 0 0
....................................................................ATAAGTTAGAGAAATTGATGTTGTC..................................................................................................... 25 1 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGTAGAGAAATTGATGTTATCTCA.................................................................................................. 28 3 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
..........................................................................................................CTTCAGTTTCATTGCCTTT..................................................................... 19 0 1 1.00 1 0 1 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGTAGAGAAATTGATGTGGTC..................................................................................................... 25 1 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
GTTTTCTGGCAATCAAAGGAAC............................................................................................................................................................................ 22 0 1 1.00 1 0 0 1 0 0 0 0 0 0 0 0
.....................................................................TAAGGTAGAGAAATTGATG.......................................................................................................... 19 0 1 1.00 1 0 1 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGGAGAGAAATTGATGTT........................................................................................................ 22 1 1 1.00 1 0 0 0 1 0 0 0 0 0 0 0
......................................................GGGGCGCTCAGGCTATAAGGTA...................................................................................................................... 22 0 1 1.00 1 0 1 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGTAGAGAAATTGATGTTGTCA.................................................................................................... 26 1 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGTAGAGAAATTGATGTTGG...................................................................................................... 24 1 1 1.00 1 0 0 1 0 0 0 0 0 0 0 0
....................................................................ATAAGGTTGAGAAATTGATGT......................................................................................................... 21 1 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGTAGAGAAATCGATGTTGTC..................................................................................................... 25 1 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGTAGAGAAATTGATGCT........................................................................................................ 22 1 1 1.00 1 0 0 0 1 0 0 0 0 0 0 0
.TTTTCTGGCAATCAAAGG............................................................................................................................................................................... 18 0 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
....................................................................CTAAGGTAGAGAAATTGATGTTGT...................................................................................................... 24 1 1 1.00 1 0 0 0 1 0 0 0 0 0 0 0
....................................................................ATAAGGTAGAGAAATTGATGTG........................................................................................................ 22 1 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
.....................................................................TAAGGTAGAGAAATTGATGTTGTC..................................................................................................... 24 0 1 1.00 1 0 0 0 0 1 0 0 0 0 0 0
....................................................................ATAAGGTAGGGAAATTGATGTT........................................................................................................ 22 1 1 1.00 1 0 0 0 0 1 0 0 0 0 0 0
.......................................................................AGGTAGAGAAATTGATGTTGTC..................................................................................................... 22 0 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
...................................................................TATAAGGTAGAGAAATTGATGT......................................................................................................... 22 0 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
....................................................................ATAGGGTAGAGAAATTGATGTTGTC..................................................................................................... 25 1 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGTAGAGAAATTGATGTTGTCTT................................................................................................... 27 1 1 1.00 1 0 0 1 0 0 0 0 0 0 0 0
....................................................................ATAAGGTAGAGAAAGTGATGTTGT...................................................................................................... 24 1 1 1.00 1 0 0 0 1 0 0 0 0 0 0 0
....................................................................AGAAGGTAGAGAAATTGATGTTG....................................................................................................... 23 1 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
..........................................................................TAGAGAAATTGATGTT........................................................................................................ 16 0 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
.....................................................................TAAGGTAGAGAAATTGATGTTG....................................................................................................... 22 0 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
............................................................................GAGAAATTGATGTTGTC..................................................................................................... 17 0 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
..........................................................................TAGAGAAATTGATGTTGTCT.................................................................................................... 20 0 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGTACAGAAATTGATGTT........................................................................................................ 22 1 1 1.00 1 0 0 0 0 1 0 0 0 0 0 0
....................................................................ATAAGGTAGAGCTATTGATGTT........................................................................................................ 22 2 1 1.00 1 0 0 0 0 0 0 0 0 1 0 0
...........................................................................AGAGAAATTGATGTTGTCT.................................................................................................... 19 0 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGTAGAGAAATTGATGCTGT...................................................................................................... 24 1 1 1.00 1 0 0 0 0 0 0 0 0 0 1 0
...........................................................................AGAGAAATTGATGTTG....................................................................................................... 16 0 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGGAGAGATCTTGATGTTGTC..................................................................................................... 25 3 1 1.00 1 0 0 0 0 0 0 0 0 1 0 0
....................................................................ATAAGGTAGAGAAATTGATGTTGTCTA................................................................................................... 27 0 1 1.00 1 0 1 0 0 0 0 0 0 0 0 0
.....................................................................TAAGGTAGCGAAATT.............................................................................................................. 15 1 9 0.78 7 4 0 0 1 0 0 0 0 0 0 0
....................................................................ATAAGGTAGAGAAATGGATGGC........................................................................................................ 22 3 2 0.50 1 1 0 0 0 0 0 0 0 0 0 0
.....................................................................ATAAGTAGAGAAATTGATGTTGT...................................................................................................... 23 3 2 0.50 1 1 0 0 0 0 0 0 0 0 0 0
..............................................................................GAAATTGATGTTGTC..................................................................................................... 15 0 2 0.50 1 1 0 0 0 0 0 0 0 0 0 0
................................................................GGCCCTAAGGTAGCGAAATT.............................................................................................................. 20 3 5 0.20 1 1 0 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGTAGAGAAATA.............................................................................................................. 16 1 6 0.17 1 1 0 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGTGGAGAAATT.............................................................................................................. 16 1 7 0.14 1 1 0 0 0 0 0 0 0 0 0 0
....................................................................ATAAGGTAGATAAATT.............................................................................................................. 16 1 8 0.13 1 1 0 0 0 0 0 0 0 0 0 0
................................................CGCGATGGTGGGCTCAGGC............................................................................................................................... 19 3 10 0.10 1 0 0 1 0 0 0 0 0 0 0 0
..................................................................................................................................................ACCATGGTAGTGATAACAT............................. 19 3 14 0.07 1 1 0 0 0 0 0 0 0 0 0 0
.....................................................................TAAGGTAGTGAAATT.............................................................................................................. 15 1 18 0.06 1 1 0 0 0 0 0 0 0 0 0 0
.......................................................................................................................................GCACCCCCGTAACAGTGA......................................... 18 3 20 0.05 1 0 0 0 1 0 0 0 0 0 0 0
..............................................................CAGGCGGTCAGGTAGAGAA................................................................................................................. 19 3 20 0.05 1 1 0 0 0 0 0 0 0 0 0 0

Anti-sense strand reads

TCCTTTGTAGTGCATTGTCTGGTCCAACGATGTTATCACTATCACTGTTGCAGGGGGGCAAACAAATTGAAAGGCAATGAAACTGAAGTCTGAATAGTGTAGACAACATCAATTTCTCTACCTTATAGCCTGAGCGCCCCATCGAGGTAGTTGTAGATTCAAGTCGAGTCGAGTTCCTTTGATTGCCAGAAAAC
****************************************************..((((((..((((....(((((((.((((((((.(((..............))).)))))))).)))))))....))))..)))))).***************************************************** 		Read size # Mismatch Hit Count Total Norm Total
................................................................................................CTGTAGGCAACATCAA.................................................................................. 16 2 20 5.50 110
................................................................................................GTGTAGGCACCATCAAT................................................................................. 17 2 10 1.60 16
................................................................................................CTGTAGACACCATCAAT................................................................................. 17 2 16 1.56 25
................................................................................................CTGTAGGCAACATCAAT................................................................................. 17 2 17 0.59 10
................................................................................................CTGTAGACACCATCAATT................................................................................ 18 2 6 0.33 2
............................................................................................AATACTGTAGGCACCATCAA.................................................................................. 20 3 5 0.20 1
.................................................................................................TGTAGACACCATCAA.................................................................................. 15 1 11 0.18 2
...........................................................AAAGAAATGGAAAGGCACTGAAA................................................................................................................ 23 3 6 0.17 1
............................................................AAGAAATGGAAAGGCACTGAAA................................................................................................................ 22 3 8 0.13 1
.......................................TATCAATGTTGCAGG............................................................................................................................................ 15 1 8 0.13 1

Re-alignment of all predicted orthologs

Species Coordinate ID Alignment
droSim3 2L:5434334-5434527 + dsi-mir-961 GTTTTCTGGCAATC-AAAGGAACTCG-ACTCG---ACT-TGAATC--------TACAACTACCTCGAT-GGGGCGCTCAGGCTATAAGGTAGAGAAATTGATGTTGTCTACACTA--TTCAGACTTCAGTTTCATTGCCTTTCAATTTGTTTGCCCCCCT--GCAACAGTGAT---AGTGATA-------------------A---CAT----CG--TTGGACC-------AGACAATGCACTACAAAGGA
droSec2 scaffold_5:3718989-3719182 + dse_179 GTTTTCTGGCAATC-AAAGGAACTCG-ACTCG---ACT-TGAATC--------TACAACTACCTCGAT-GGGGCATTCAGGCTATAAGGTAGAGAAATTGATGTTGTCTACACTA--TTCAGACTTCAGTTTCATTGCCTTTCAATTTGTTTGCCCCCAT--GCAACAGTGGT---AGTGATA-------------------A---CAT----CG--TTGGACC-------AGACAATGCACTACAAAGGA
dm3 chr2L:5641248-5641443 + dme-mir-961 GTTTTCTGGCAATC-AAAAGAACTTGGACTCG---ACT-TGAATT----------CAACTACTTCGAT-GGGGCACTCAGGCTATAAGGTAGAGAAATTGATGCTGTCTACACTA--TTCAGACTTCAGTTTCATTACCTTTCAATTTGTTTGCCCCCAT--GCAACAGTGAT---AGTAGTG----------------ATAA---AAT----CG--TTGGACC-------AGACAATACACTACAAAGGA
droEre2 scaffold_4929:5729710-5729900 + der_35 GTTTCCTGGCGATC-AAAAGAACTCG-ACTCG---GCT-CGAATC----------CCAGTACCTCCTT-GGGGCACTCAAGCTATAAGGTAGAAAAATTGTTGTCTTCCACACTA--TTCGGACTGCAGTTTAACTATCTTTCAATTTGTTTGCCCCCAC--GCAACAGTGAC---AACGATA-------------------A---CAT----CG--TTGGGCC-------ACACAATACACTACAA-GGG
droYak3 2L:13551875-13552066 - dya_33 GTTTTCTGGCGATC-AAAAGAACTCG-ACTCG---ACT-CGAATC----------CAACCAACTTCAT-GGGGCACACAAGCTATAAGGTAGAGAAATTGTTGTCTTCCACACTA--TTCAGACATCAGTTTAACTATCTTTCAATTTGTTTGCCCCCAC--GCAACAGTGAT---AATGATA-------------------A---CAT----CG--TTGGGCC-------ACACAATGCACTACAAAGGA
droEug1 scf7180000409063:317561-317751 + GTTTTCTGGCGATC-AAACAAACTCG-ACTTCAAAACC-CAAACC----------CAACTTCCTCGCC-GGGGCACACAAGCTCTAAGGTAGAGAAAATGTTGTCTTTGACGCGT--TCTAGACATCAATTTCCCTAGCTTTCAATTTGTCTGCCCCCAC--GCAACCGGGAT---ACCGATA-------------------A---CG----------TGGACA-------TTACAATCCTGCACAAAGGA
droBia1 scf7180000302422:3830803-3830989 + GTTTTCTGGCGATC-AAACAAACTCG-ACCCAAAG--------GC----------CAACCAACCCG-T-CGGGCACTCAAGCTATAAGGTAGAGAAATTGTTGTCCCACACTCTA--TTCAGACATCAATTTCACTAGCTTTCAATTTGACTGCCCCCAT--GCATCAGTGAT---AATGATA-------------------A---CAT----CG--CTGGACA-------TAACAATTCGGTACAAAGGG
droTak1 scf7180000415871:385540-385726 + GTTTTCTGGCGATC-AAATAAACTCG-ACTTAAAA--------CC----------CAACCAACTCGAC-GGGGCACCCAAGCTATAAGGTAGAGAAACTGTTGTCTGGCACTCAA--TTCAGACATCAATTTCACTAGCTTTCAATTTGTCTGCCCCCAC--GCAACAGTGAT---AATGATA-------------------A---GAT----CG--TTGGACG--------TACAATATGGCACAAAGGG
droEle1 scf7180000490753:30444-30620 + GTTTTCTGGAGATC-AAATAAACTCG-AA--------------CC----------CAATTACCTC----GGGGCACCCAAGCTATAAGGTAGAGAAAATGTTGTCTTCC-CACTA--CTCAGACATCAATTTCACTAGCTTTCAATTTGTCTGCCC-CAC--GCAACAGTGAT---AATGATA-------------------G---CAT----CG--TTTGGCA-------TTACAATCCAGCACAATGGG
droFic1 scf7180000453932:197404-197586 - GTTTTCTGGCGATC-AAACAAACTCG-GCTCGGAA--------GC----------CATCC--CCCCAC-TGGGCAGTCAAGCTATAAGGTAGAGAAACTCTTGTCTTCCCCTACA--TCCAGACATCAGTTTCACTAGCTTTCAATTTGTCTGCCCCCAC--GCAACAA-GAT---AGTGATT-------------------A---C-C----CG--ATGGACA-------TTTCCATTCAGCGCTACG-G
droKik1 scf7180000302389:430798-430990 - GTTTTCTGGCGATC-AAACAAACTCG-TCTCGGAA-CT-CCAA-----CATCCTGGAACTCCCTGCCC-AAGGCACTCAAGCTATAAGGTAGAGAAATGGCTGTCTT-GACTCTAAGCTCGGACATCAATTTCACTAGCTTTCAATTTGTCTGCCCCCAC--GCAACAGTGAT---AATGTTA-------------------A---CATGGCCCGAACTG--------------------GACACAATGGG
droBip1 scf7180000396572:742807-742977 + ATTTTCTGGCTATC-AAATACACACG-ACTTTGGG--------CC----------CAACCACCTGGTCGGGGGCACCCAAGCTATAAGGTAGAGAAAGTAATGTCTTCGTCTCCA--AATAGACATCAATTTCCCTAGCTTGCAGTTTGCTTGCCCTCAA--GCAACCATGAT---AATAATA-------------------AGCTCAT----CA--GTGAAC----------------------------
dp5 4_group4:3182159-3182379 - dps-mir-2509 TTATTCTAGTGATTGAAATGAACTCG-ACTCGAGTGCCACAAATGCCACATGATGCA--------AAT-GGAGCAGTCGTGTTTTAAGGTACAGACTTT---------GAGTCTAT-TCTAGGCATCAATTTTCGTATCTTTTAATTCGTCTGGCGCCAACAGCAACAGCTACAAAACGAGTAACGAGGAGAGATAATGATCA---TGT----CG--TTTCATTTTGGTTAGTATTAT-TAACACAAAGGG
droPer2 scaffold_10:2201090-2201310 - dpe_318 TTATTCTAGTGATTGAAATGAACTCG-ACTCGAGTGCCACAAATGCCACATGATGCA--------AAT-GGAGCAGTCGTGTTTTAAGGTACAGACTTT---------GAGTCTAT-TCTAGGCATCAATTTTCGTATCTTTTAATTCGTCTGCCGCCAACAGCAACAGCTACAAAACGAGTAACGAGGAGAGATAATGACCA---TGT----CG--TTTCATTTTGGTTAGTATTAT-TAACACAAAGGG

Re-alignment of all predicted orthologs with sRNA reads

Species Read alignment
droSim3
GTTTTCTGGCAATC-AAAGGAACTCG-ACTCG---ACT-TGAATC--------TACAACTACCTCGAT-GGGGCGCTCAGGCTATAAGGTAGAGAAATTGATGTTGTCTACACTA--TTCAGACTTCAGTTTCATTGCCTTTCAATTTGTTTGCCCCCCT--GCAACAGTGAT---AGTGATA-------------------A---CAT----CG--TTGGACC-------AGACAATGCACTACAAAGGASizeHit CountTotal NormTotalV044
Embryo		M023
Head		M024
Male-body		M025
Embryo		O001
Testis		O002
Head		M053
Female-body		SRR553485
Ovary		SRR553486
Ovary		SRR553487
Ovary		SRR553488
Ovary		SRR618934
Ovary
...................................................................................ATAAGGTAGAGAAATTGATGTTGTC...............................................................................................................................................251522.0052260243196515223900122
...................................................................................ATAAGGTAGAGAAATTGATGTT..................................................................................................................................................221404.00404312209713367210168
...................................................................................ATAAGGTAGAGAAATTGATGTTGT................................................................................................................................................241244.0024400400203283700134
...................................................................................ATAAGGTAGAGAAATTGATGTTGTCT..............................................................................................................................................261206.002061052210116371095
...................................................................................ATAAGGTAGAGAAATTGATGTTG.................................................................................................................................................231148.001480020641110130057
...................................................................................ATAAGGTAGAGAAATTGATG....................................................................................................................................................201128.00128003011410000010
...................................................................................ATAAGGTAGAGAAATTGA......................................................................................................................................................18197.00970000950000002
...................................................................................ATAAGGTAGAGAAATTGAT.....................................................................................................................................................19177.00770000742000001
...................................................................................ATAAGGTAGAGAAATTGATGT...................................................................................................................................................21175.007500204730330017
..........................................................................................................................ACTTCAGTTTCATTGCCTTTCA...........................................................................................................22140.00400010380010000
...................................................................................ATAAGGTAGAGAAATT........................................................................................................................................................16138.00380000000000038
..........................................................................................................................ACTTCAGTTTCATTGCCTTTC............................................................................................................21118.00180000180000000
..........................................................................................................................ACTTCAGTTTCATTGCCT...............................................................................................................18117.00170000161000000
...........................................................................................................................CTTCAGTTTCATTGCCTTTCA...........................................................................................................2118.008003040001000
...................................................................................ATAAGGTAGAGAAAT.........................................................................................................................................................1518.008000000000008
...........................................................................................................................CTTCAGTTTCATTGCCTTTCAA..........................................................................................................2217.007001050001000
..........................................................................................................................ACTTCAGTTTCATTGCCTT..............................................................................................................1915.005000050000000
...........................................................................................................................CTTCAGTTTCATTGCCTT..............................................................................................................1815.005000050000000
....................................................................................TAAGGTAGAGAAATTGATGTTGTCT..............................................................................................................................................2514.004002000000002
..........................................................................................................................ACTTCAGTTTCATTGCCTTT.............................................................................................................2013.003000030000000
...................................................................................ATAAGGTAGAGAAATTG.......................................................................................................................................................1712.002000000000002
.....................................................................................AAGGTAGAGAAATTGATG....................................................................................................................................................1812.002000020000000
....................................................................................TAAGGTAGAGAAATTGATGTTGT................................................................................................................................................2312.002000000001001
..........................................................................................................................ACTTCAGTTTCATTGCCTTTCAA..........................................................................................................2311.001000000001000
..........................................................................................AGAGAAATTGATGTTGTC...............................................................................................................................................1811.001000000000001
.........................................................................................TAGAGAAATTGATGTTGT................................................................................................................................................1811.001000000000001
.....................................................................................AAGGTAGAGAAATTGATGTTG.................................................................................................................................................2111.001000010000000
GTTTTCTGGCAATC-AAAGG.......................................................................................................................................................................................................................................1911.001000000000001
.........................................................................................TAGAGAAATTGATGTTGTC...............................................................................................................................................1911.001000000000001
..........................................................................................AGAGAAATTGATGTTGT................................................................................................................................................1711.001000000000001
......................................................................................................................TCAGACTTCAGTTTCATTGCCT...............................................................................................................2211.001000100000000
...........................................................................................................................CTTCAGTTTCATTGCCTTTC............................................................................................................2011.001000010000000
..TTTCTGGCAATC-AAAGG.......................................................................................................................................................................................................................................1711.001000000000001
...........................................................................................................................CTTCAGTTTCATTGCCTTT.............................................................................................................1911.001000010000000
GTTTTCTGGCAATC-AAAGGAAC....................................................................................................................................................................................................................................2211.001001000000000
....................................................................................TAAGGTAGAGAAATTGATG....................................................................................................................................................1911.001000010000000
.....................................................................GGGGCGCTCAGGCTATAAGGTA................................................................................................................................................................2211.001000010000000
.TTTTCTGGCAATC-AAAGG.......................................................................................................................................................................................................................................1811.001000000000001
....................................................................................TAAGGTAGAGAAATTGATGTTGTC...............................................................................................................................................2411.001000000010000
......................................................................................AGGTAGAGAAATTGATGTTGTC...............................................................................................................................................2211.001000000000001
..................................................................................TATAAGGTAGAGAAATTGATGT...................................................................................................................................................2211.001000000000001
.........................................................................................TAGAGAAATTGATGTT..................................................................................................................................................1611.001000000000001
....................................................................................TAAGGTAGAGAAATTGATGTTG.................................................................................................................................................2211.001000000000001
...........................................................................................GAGAAATTGATGTTGTC...............................................................................................................................................1711.001000000000001
.........................................................................................TAGAGAAATTGATGTTGTCT..............................................................................................................................................2011.001000000000001
..........................................................................................AGAGAAATTGATGTTGTCT..............................................................................................................................................1911.001000000000001
..........................................................................................AGAGAAATTGATGTTG.................................................................................................................................................1611.001000000000001
...................................................................................ATAAGGTAGAGAAATTGATGTTGTCTA.............................................................................................................................................2711.001000010000000
.............................................................................................GAAATTGATGTTGTC...............................................................................................................................................1520.501000000000001
droSec2
GTTTTCTGGCAATC-AAAGGAACTCG-ACTCG---ACT-TGAATC--------TACAACTACCTCGAT-GGGGCATTCAGGCTATAAGGTAGAGAAATTGATGTTGTCTACACTA--TTCAGACTTCAGTTTCATTGCCTTTCAATTTGTTTGCCCCCAT--GCAACAGTGGT---AGTGATA-------------------A---CAT----CG--TTGGACC-------AGACAATGCACTACAAAGGASizeHit CountTotal NormTotalV113
Male-body		V114
Embryo		V115
Head		M054
Female-body
...................................................................................ATAAGGTAGAGAAATTGATGTT..................................................................................................................................................221819.0081964915767
...................................................................................ATAAGGTAGAGAAATTGATGTTGT................................................................................................................................................241764.007646976142
...................................................................................ATAAGGTAGAGAAATTGATGTTGTC...............................................................................................................................................251754.0075454220093
...................................................................................ATAAGGTAGAGAAATTGATGTTGTCT..............................................................................................................................................261486.0048635213040
...................................................................................ATAAGGTAGAGAAATTGATGTTG.................................................................................................................................................231393.003933761034
...................................................................................ATAAGGTAGAGAAATTGATGT...................................................................................................................................................21128.002822402
....................................................................................TAAGGTAGAGAAATTGATGTTGTCT..............................................................................................................................................2519.0096201
...................................................................................ATAAGGTAGAGAAATTGATG....................................................................................................................................................2017.0077000
....................................................................................TAAGGTAGAGAAATTGATGTTGTC...............................................................................................................................................2414.0044000
....................................................................................TAAGGTAGAGAAATTGATGTTGT................................................................................................................................................2314.0043100
....................................................................................TAAGGTAGAGAAATTGATGTTG.................................................................................................................................................2213.0033000
...................................................................................ATAAGGTAGAGAAATTGA......................................................................................................................................................1811.0010100
....................................................................................TAAGGTAGAGAAATTGATGTT..................................................................................................................................................2111.0010100
......................................................................................AGGTAGAGAAATTGATGTT..................................................................................................................................................1911.0011000
...................................................................................ATAAGGTAGAGAAATTGATGTTGTCTA.............................................................................................................................................2711.0011000
droEre2
GTTTCCTGGCGATC-AAAAGAACTCG-ACTCG---GCT-CGAATC----------CCAGTACCTCCTT-GGGGCACTCAAGCTATAAGGTAGAAAAATTGTTGTCTTCCACACTA--TTCGGACTGCAGTTTAACTATCTTTCAATTTGTTTGCCCCCAC--GCAACAGTGAC---AACGATA-------------------A---CAT----CG--TTGGGCC-------ACACAATACACTACAA-GGGSizeHit CountTotal NormTotalV040
Embryo		V060
Head		V107
Male-body		V108
Head		M055
Female-body		M057
Embryo
...................................................................................ATAAGGTAGAAAAATTGTTGTC..................................................................................................................................................2217500.00750033567007292211
...................................................................................ATAAGGTAGAAAAATTGTTGT...................................................................................................................................................211605.00605015495131
...................................................................................ATAAGGTAGAAAAATTGTTGTCT.................................................................................................................................................231300.00300042583170
...................................................................................ATAAGGTAGAAAAATTGTTG....................................................................................................................................................20182.00820171910
....................................................................................TAAGGTAGAAAAATTGTTGTCT.................................................................................................................................................22168.00680065210
....................................................................................TAAGGTAGAAAAATTGTTGTC..................................................................................................................................................2117.007005110
.....................................................................................AAGGTAGAAAAATTGTTGTC..................................................................................................................................................2016.006006000
....................................................................................TAAGGTAGAAAAATTGTTGTCTTC...............................................................................................................................................2411.001001000
...........................................................................................................................CTGCAGTTTAACTATCTTTCA...........................................................................................................2111.001001000
....................................................................................TAAGGTAGAAAAATTGTTGT...................................................................................................................................................2011.001001000
....................................................................................TAAGGTAGAAAAATTGTTGTCTT................................................................................................................................................2311.001000100
droYak3
GTTTTCTGGCGATC-AAAAGAACTCG-ACTCG---ACT-CGAATC----------CAACCAACTTCAT-GGGGCACACAAGCTATAAGGTAGAGAAATTGTTGTCTTCCACACTA--TTCAGACATCAGTTTAACTATCTTTCAATTTGTTTGCCCCCAC--GCAACAGTGAT---AATGATA-------------------A---CAT----CG--TTGGGCC-------ACACAATGCACTACAAAGGASizeHit CountTotal NormTotalV046
Embryo		V052
Head		V120
Male-body		V058
Head		M026
Head		M043
Female-body		M056
Embryo
...................................................................................ATAAGGTAGAGAAATTGTTGTC..................................................................................................................................................2211280.00128011115816305123
...................................................................................ATAAGGTAGAGAAATTGTTGTCT.................................................................................................................................................231633.0063300600213144
...................................................................................ATAAGGTAGAGAAATTGTTGT...................................................................................................................................................211129.001290010721181
....................................................................................TAAGGTAGAGAAATTGTTGTCT.................................................................................................................................................22145.004500430020
...................................................................................ATAAGGTAGAGAAATTGTTG....................................................................................................................................................20123.002300201020
....................................................................................TAAGGTAGAGAAATTGTTGTC..................................................................................................................................................2112.0020000020
.....................................................................................AAGGTAGAGAAATTGTTGTCT.................................................................................................................................................2111.0010010000
...........................................................................................................................CATCAGTTTAACTATCTTTCA...........................................................................................................2111.0010000010
..................................................................................TATAAGGTAGAGAAATTGTTGTCT.................................................................................................................................................2411.0010010000
......................................................................................AGGTAGAGAAATTGTTGTC..................................................................................................................................................1911.0010010000
...................................................................................ATAAGGTAGAGAAATTGT......................................................................................................................................................1811.0010100000
...................................................................................ATAAGGTAGAGAAATTGTT.....................................................................................................................................................1911.0010010000
.....................................................................................AAGGTAGAGAAATTGTTGTC..................................................................................................................................................2011.0010000001
...................................................................................ATAAGGTAGAGAAATTGTTGTCTT................................................................................................................................................2411.0010010000
droEug1
GTTTTCTGGCGATC-AAACAAACTCG-ACTTCAAAACC-CAAACC----------CAACTTCCTCGCC-GGGGCACACAAGCTCTAAGGTAGAGAAAATGTTGTCTTTGACGCGT--TCTAGACATCAATTTCCCTAGCTTTCAATTTGTCTGCCCCCAC--GCAACCGGGAT---ACCGATA-------------------A---CG----------TGGACA-------TTACAATCCTGCACAAAGGASizeHit CountTotal NormTotal
No Reads
droBia1
GTTTTCTGGCGATC-AAACAAACTCG-ACCCAAAG--------GC----------CAACCAACCCG-T-CGGGCACTCAAGCTATAAGGTAGAGAAATTGTTGTCCCACACTCTA--TTCAGACATCAATTTCACTAGCTTTCAATTTGACTGCCCCCAT--GCATCAGTGAT---AATGATA-------------------A---CAT----CG--CTGGACA-------TAACAATTCGGTACAAAGGGSizeHit CountTotal NormTotal
No Reads
droTak1
GTTTTCTGGCGATC-AAATAAACTCG-ACTTAAAA--------CC----------CAACCAACTCGAC-GGGGCACCCAAGCTATAAGGTAGAGAAACTGTTGTCTGGCACTCAA--TTCAGACATCAATTTCACTAGCTTTCAATTTGTCTGCCCCCAC--GCAACAGTGAT---AATGATA-------------------A---GAT----CG--TTGGACG--------TACAATATGGCACAAAGGGSizeHit CountTotal NormTotal
No Reads
droEle1
GTTTTCTGGAGATC-AAATAAACTCG-AA--------------CC----------CAATTACCTC----GGGGCACCCAAGCTATAAGGTAGAGAAAATGTTGTCTTCC-CACTA--CTCAGACATCAATTTCACTAGCTTTCAATTTGTCTGCCC-CAC--GCAACAGTGAT---AATGATA-------------------G---CAT----CG--TTTGGCA-------TTACAATCCAGCACAATGGGSizeHit CountTotal NormTotal
No Reads
droFic1
GTTTTCTGGCGATC-AAACAAACTCG-GCTCGGAA--------GC----------CATCC--CCCCAC-TGGGCAGTCAAGCTATAAGGTAGAGAAACTCTTGTCTTCCCCTACA--TCCAGACATCAGTTTCACTAGCTTTCAATTTGTCTGCCCCCAC--GCAACAA-GAT---AGTGATT-------------------A---C-C----CG--ATGGACA-------TTTCCATTCAGCGCTACG-GSizeHit CountTotal NormTotal
No Reads
droKik1
GTTTTCTGGCGATC-AAACAAACTCG-TCTCGGAA-CT-CCAA-----CATCCTGGAACTCCCTGCCC-AAGGCACTCAAGCTATAAGGTAGAGAAATGGCTGTCTT-GACTCTAAGCTCGGACATCAATTTCACTAGCTTTCAATTTGTCTGCCCCCAC--GCAACAGTGAT---AATGTTA-------------------A---CATGGCCCGAACTG--------------------GACACAATGGGSizeHit CountTotal NormTotal
No Reads
droBip1
ATTTTCTGGCTATC-AAATACACACG-ACTTTGGG--------CC----------CAACCACCTGGTCGGGGGCACCCAAGCTATAAGGTAGAGAAAGTAATGTCTTCGTCTCCA--AATAGACATCAATTTCCCTAGCTTGCAGTTTGCTTGCCCTCAA--GCAACCATGAT---AATAATA-------------------AGCTCAT----CA--GTGAAC----------------------------SizeHit CountTotal NormTotal
No Reads
dp5
TTATTCTAGTGATTGAAATGAACTCG-ACTCGAGTGCCACAAATGCCACATGATGCA--------AAT-GGAGCAGTCGTGTTTTAAGGTACAGACTTT---------GAGTCTAT-TCTAGGCATCAATTTTCGTATCTTTTAATTCGTCTGGCGCCAACAGCAACAGCTACAAAACGAGTAACGAGGAGAGATAATGATCA---TGT----CG--TTTCATTTTGGTTAGTATTAT-TAACACAAAGGGSizeHit CountTotal NormTotalV112
Male-body		V043
Embryo		V051
Head		M022
Male-body		M040
Female-body		M059
Embryo		M062
Head
..................................................................................TTTAAGGTACAGACTTT---------GAGTC..........................................................................................................................................221234.002342010886110
..................................................................................TTTAAGGTACAGACTTT---------GAG............................................................................................................................................20135.003526024300
..................................................................................TTTAAGGTACAGACTTT---------GAGTCT.........................................................................................................................................23133.003380215800
..................................................................................TTTAAGGTACAGACTTT---------GAGT...........................................................................................................................................21113.001310110100
............................................................................................................................ATCAATTTTCGTATCTTTTAA..........................................................................................................2113.0030000300
...................................................................................TTAAGGTACAGACTTT---------GAGTC..........................................................................................................................................2113.0033000000
..................................................................................TTTAAGGTACAGACTTT---------GA.............................................................................................................................................1912.0020010100
...........................................................................................................................CATCAATTTTCGTATCTTTTAAT.........................................................................................................2311.0010000001
.......................................................................AGCAGTCGTGTTTTAAGGTAC...............................................................................................................................................................2111.0010001000
...................................................................................TTAAGGTACAGACTTT---------GAGTCT.........................................................................................................................................2211.0010000100
droPer2
TTATTCTAGTGATTGAAATGAACTCG-ACTCGAGTGCCACAAATGCCACATGATGCA--------AAT-GGAGCAGTCGTGTTTTAAGGTACAGACTTT---------GAGTCTAT-TCTAGGCATCAATTTTCGTATCTTTTAATTCGTCTGCCGCCAACAGCAACAGCTACAAAACGAGTAACGAGGAGAGATAATGACCA---TGT----CG--TTTCATTTTGGTTAGTATTAT-TAACACAAAGGGSizeHit CountTotal NormTotalV111
Male-body		V042
Embryo		V050
Head		V057
Head		M021
Embryo		M042
Female-body
..................................................................................TTTAAGGTACAGACTTT---------GAGTC..........................................................................................................................................221234.0023423201001
..................................................................................TTTAAGGTACAGACTTT---------GAGTCT.........................................................................................................................................23137.00373700000
..................................................................................TTTAAGGTACAGACTTT---------GAG............................................................................................................................................20132.00323200000
..................................................................................TTTAAGGTACAGACTTT---------GAGT...........................................................................................................................................21115.00151400001
..................................................................................TTTAAGGTACAGACTTT---------GA.............................................................................................................................................1915.005500000

Generated: 12/06/2013 at 02:36 PM