dsi-mir-2583

ATTTAATTGATCCGCACAAGTTCATATCCGTGTAATTTGTGCCCGTCTCTTTTGCAGTGTGTCAAAGTTGAGTCGATAATGCGATCATCTTGTATAATGATCGTGCAATGGACTCAATTTCGACTCGGCAAACATCATCAAAGCCTAATTGATTGCAGGCACCCCTCGAATTGCCCCGCCTT **********************************************..(((((..((.(((.((((((((((.((..(((((((((........)))))))))..)).)))))))))).))).))))))).....*******************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M023 head	M024 male body	SRR553486 Ovary	M025 embryo	SRR618934 Ovary	SRR553485 Ovary	SRR553488 Ovary	O002 Head	GSM343915 embryo	O001 Testis	SRR553487 Ovary	M053 Female-body
....................................................................................................TCGTGCAATGGACTCAATTTCG............................................................	22	0	1	73.00	73	35	10	6	9	0	8	4	0	1	0	0	0
...............................................................AAAGTTGAGTCGATAATGCGAT.................................................................................................	22	0	1	37.00	37	7	1	5	4	11	0	4	0	2	0	3	0
...............................................................AAAGTTGAGTCGATAATGCGA..................................................................................................	21	0	1	26.00	26	6	3	7	0	0	4	2	2	1	0	1	0
....................................................................................................TCGTGCAATGGACTCAATTTC.............................................................	21	0	1	12.00	12	7	4	0	0	0	0	0	1	0	0	0	0
...............................................................AAAGTTGAGTCGATAATGCGATC................................................................................................	23	0	1	4.00	4	2	0	0	0	0	0	0	1	0	1	0	0
...............................................................AAAGTTGAGTCGATAATGC....................................................................................................	19	0	1	4.00	4	1	0	0	0	0	0	0	2	0	1	0	0
.....................................................................................................CGTGCAATGGACTCAATTTCGA...........................................................	22	0	1	3.00	3	1	0	0	1	0	0	0	0	1	0	0	0
..................................................................GTTGAGTCGATAATGCGATC................................................................................................	20	0	1	3.00	3	2	0	0	0	1	0	0	0	0	0	0	0
...............................................................AAAGTTGAGTCGATAATGCG...................................................................................................	20	0	1	3.00	3	2	0	0	0	0	0	0	1	0	0	0	0
.....................................................................................................CGTGCAATGGACTCAATTTCG............................................................	21	0	1	2.00	2	2	0	0	0	0	0	0	0	0	0	0	0
....................................................................................................TCGTGCAATGGACTCAATTTCGG...........................................................	23	1	1	2.00	2	0	0	0	2	0	0	0	0	0	0	0	0
....................................................................................................TCGTGCAATGGACTCAAT................................................................	18	0	1	2.00	2	1	0	0	0	0	0	0	0	0	1	0	0
...............................................................AAAGTTGAGTCGATAATGCGATA................................................................................................	23	1	1	2.00	2	0	0	0	0	0	2	0	0	0	0	0	0
................................................................AAGTTGAGTCGATAATGCGATC................................................................................................	22	0	1	2.00	2	1	0	0	0	0	0	0	0	0	0	0	1
....................................................................................................TCGTGCCATGGACTCAATTTC.............................................................	21	1	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	1
..................................................................GTTGAGTCGATAATGCGGTGCT..............................................................................................	22	3	1	1.00	1	0	0	0	0	0	0	0	0	1	0	0	0
....................................................................................................TCGTGCAATGGACTCAATTTCGA...........................................................	23	0	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0	0
................................................................CAGTTGAGTCGATAATGCGATC................................................................................................	22	1	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0
..................................................................................................GATCGTGCAATGGACTCAAT................................................................	20	0	1	1.00	1	0	0	0	0	0	0	0	0	0	1	0	0
....................................................................................................TCGTGCAATGGACTCAACTTCG............................................................	22	1	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0
....................................................TGCAGTGTGTCAAAGTTGAGT.............................................................................................................	21	0	1	1.00	1	0	0	0	0	0	0	0	0	1	0	0	0
...............................................................AAAGTTGAGTCGATAATGCGAC.................................................................................................	22	1	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0
.....................................................................................................CGTGCAATGGACTCAATTTC.............................................................	20	0	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0
...............................................................AAAGTTGAGTCGATAATGCGATT................................................................................................	23	1	1	1.00	1	0	0	0	0	0	0	0	0	1	0	0	0
....................................................................................................CCGTGCAATGGACTCAATTTCG............................................................	22	1	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0
...............................................................................TGAGATAATCTTGTATGATGAT.................................................................................	22	3	1	1.00	1	0	0	0	0	0	0	0	0	1	0	0	0
..................................................................GTTGAGTCGATAATGCGATCA...............................................................................................	21	0	1	1.00	1	0	0	0	0	0	0	0	0	1	0	0	0
...............................................................AAAGTTGAGTCGATAATGCGTAA................................................................................................	23	3	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0
...............................................................AAAGTTGAGTCGATAATGCGG..................................................................................................	21	1	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0
...............................................................AAAGTTGAGTCGATAATCCGAT.................................................................................................	22	1	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
...................................................................................................ATCGTGCAATGGACTCAATTTCG............................................................	23	0	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0
..................................................................GTTGAGTCGATAATGCGATTAT..............................................................................................	22	1	1	1.00	1	0	0	0	0	0	0	0	0	1	0	0	0
...................................................................................................ATCGTGCAATGGACTCAATTT..............................................................	21	0	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0
...................................................................TTGAGTCGATAATGCGAT.................................................................................................	18	0	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
.....................................................................................................CGTGCAATGGACTCAATTTCGT...........................................................	22	1	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0
..................................................................GTTGAGTCGATAATGCGA..................................................................................................	18	0	1	1.00	1	0	0	0	0	0	0	0	1	0	0	0	0
...................................................................................................ATCGTGCAATGGACTCAATTTC.............................................................	22	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0
...............................................................................TGAGATCATTTTGTATGATGAT.................................................................................	22	3	6	0.67	4	0	0	0	0	0	0	0	0	4	0	0	0
...............................................................................TGTGATCATTTTGTATATTGAT.................................................................................	22	3	5	0.20	1	0	0	0	0	0	0	0	0	1	0	0	0
...............................................................................TGCGATCATTTTGTATGCTGAT.................................................................................	22	3	5	0.20	1	0	0	0	0	0	0	0	0	1	0	0	0
.................................................................................................TGACCGTGGAATGAACTCA..................................................................	19	3	16	0.06	1	0	0	1	0	0	0	0	0	0	0	0	0
......................................GTGGCCGTCTCTTTT.................................................................................................................................	15	1	20	0.05	1	0	0	0	0	1	0	0	0	0	0	0	0

AAGGCGGGGCAATTCGAGGGGTGCCTGCAATCAATTAGGCTTTGATGATGTTTGCCGAGTCGAAATTGAGTCCATTGCACGATCATTATACAAGATGATCGCATTATCGACTCAACTTTGACACACTGCAAAAGAGACGGGCACAAATTACACGGATATGAACTTGTGCGGATCAATTAAAT *******************************************.....(((((((.(((.((((((((((.((..(((((((((........)))))))))..)).)))))))))).))).))..)))))..**********************************************	Read size	# Mismatch	Hit Count	Total Norm	Total
.......................................CTTTGACGAGGTTTGGCGAG...........................................................................................................................	20	3	5	0.20	1
........................................................................................TACACGATGAGCGCATTGT...........................................................................	19	3	9	0.11	1
.......................................................GGAGTCGAGACTGAGTCCA............................................................................................................	19	3	15	0.07	1
...............................................ATGTTGGACGAGTCGGAAT....................................................................................................................	19	3	17	0.06	1

Re-alignment of all predicted orthologs

Species	Coordinate	ID	Alignment
droSim3	X:5118637-5118818 +	dsi-mir-2583	ATTTAATTGATCC-----GCACAAGTTC------------------------------------------------ATATC----CGTGTAATTTGTG-------CCCGTCTCTTTT----GC-AG--TGTGTCAAAGTTGAG----------------TCGATAATGCGATCATCTTGT----------------------------------------ATAATGATCG-TGCAATGGACTCAATTTC--G--ACTCGGCA---------------AACATCATCAA-AGCCTAATTGATTGCAG--GCACCCCTCGAATTGCCCCGCCTT
droSec2	scaffold_4:290350-290531 -	dse_343	ATTTAATTGATCC-----GCACAAGTTC------------------------------------------------ATATC----CGTGTAATTTGTG-------CCCGTCTCTTTT----GC-AG--TGTGTCAAAGTTGAG----------------TCGATAATGCGATCATCTTGT----------------------------------------ATAATGATCG-TGCAATGGACTCAATTTC--G--ACTCGGCA---------------AACATCATCAA-AGCCTAATTGATTGCAG--GCACCCCTCGAATTGCCCCGCCTT
dm3	chrX:6514050-6514235 +	dme_165	ATTTAATTGATCC-----GCACAAGTTC------------------------------------------------ATATC----CGTGTAATTTGTG-------CCCGTCTCTTTTGCTTGC-AG--TGTGTCAAAGTTGAG----------------ACGATAATGCGATCATCTTGT----------------------------------------ATAATGATCG-TGCAATGGACTCAATTTC--G--ACTCGGCA---------------AACATCATCAA-AGCCTAATTGATTGCAG--GCACCCCACGAATTGCCCCGCCTT
droEre2	scaffold_4690:15441403-15441586 +	der_218	ATTTAATTGATCC-----GCACAAGTTC------------------------------------------------ATATC----CGTGTAATTTGTGCG-----CCAGTCTCTTTT----GC-AG--TGTGTCAAAGTTGAG----------------TCGATAATGCGATCATCTTGT----------------------------------------ATAATGATCG-TGCAATGGACTCAATTTC--G--ACTCGGCA---------------AACATCATCAA-AACCTAATTGATTGCAG--GCACCCTTCAAATTGCCCCGCCTT
droYak3	X:2477318-2477499 +		ATTTAATTGATCC-----GCACAAGTTC------------------------------------------------ATATC----CGTGTAATTTGTG-------CCAGTCTCTTTT----GC-AG--TGTGTCAAAGTTGAG----------------ACGATAATGCGATCATCTTGT----------------------------------------ATAATGATCG-TGCAATGGACTCAATTTC--G--ACTCGGCA---------------AACATCATCAA-AACCTAATTGATTGCAG--GCACCCCTTAAATTGCCCCGCCCT
droEug1	scf7180000409522:182177-182360 +		ATTTAATTGATCC-----GCACAAGTTC------------------------------------------------ATATC----CGTGTAATTTGTG-------CCCGTCTCTTTT----GC-AGC-TGTGTCAAAGTTGAG----------------TTGATAATGCGATCATCTTGT----------------------------------------ATAATGATCA-TGCAATGAACTCAATTTC--A--ACTCGGCA---------------AACATCATCAAAAAACTAATTGATTGCAG--GCACCCCTCAAATTGCCCCGCCTG
droBia1	scf7180000301760:2893528-2893722 -		ACCTAATTGATCC-----GCACAAGTTC------------------------------------------------ATATC----CGTGTAAT-TGCG-------CCCGTCTCCCCG----GC-AG--GGC-CCAGAGCTGAGGGCATTGGCTGTGGCGTCGATAACGCGATCATCTTGT----------------------------------------ATGGTGATCG-TGCAATGGACTCAATTCC-----CCTCGGCA---------------GACATCATCGG-AAACTAATTGATGGCGG--GCACCCCTCGCTCGGCCCCGCCCG
droTak1	scf7180000414549:120928-121109 +		ATTTAAGTGATCC-----GCACAAGTTC------------------------------------------------ATATC----CGTGTAATTTGCG-------CCCCTCTCTTTT----GC-AG--TGTGTCAAAGTTGAG----------------TTGATAATGCGATCATCTTGT----------------------------------------ATAATGATCG-TGCAATGGACTCAATTTC--G--AACCGGCA---------------AACATCATCAA-AAACTAATTGATTGCAG--GCAAGCCTCAAATTGCCCCGCCTT
droEle1	scf7180000491044:397260-397441 +		ATTTAATTGATCC-----GCACAAGTTC------------------------------------------------ATATC----CGTGTAATTTGTG-------CCAGTCTCTTTC----GC-AG--TGTGTCAAAGTTGAG----------------TTGATAATGCGATCATCTTGT----------------------------------------ATAATGATCG-TGCAATGGACTCAATTTC--G--TCTCGGCA---------------AACATCATCAA-AAACTAATTGATTGCAG--GCACCCCTCAAATTGCCCCGCCTG
droRho1	scf7180000776942:79229-79410 +		ATTTAATTGATCC-----GCACAAGTTC------------------------------------------------ATATC----CGTGTAATTTGTG-------CCAGTCTCTTTT----GC-AG--TATGTCAAAGTTAAG----------------TTGATAATGCGATCATCTTGT----------------------------------------ATAATGATCG-TGCAATGGACTCAATTTC--G--ACTCGGCA---------------AACATCATCAA-AAACTAATTGATTGTAG--GCACCCCTCAAATTGCCCCGCCTG
droFic1	scf7180000454072:901517-901699 -		ATTTAATTGATCC-----GCACAAGTTC------------------------------------------------ATATC----CGTGTAATTTGTG-------CCCGTCTCTTTC----GC-AG--TGTGTCAAAGTTGAG----------------TCGATAATGCGATCATCTTGT----------------------------------------ATAATGATCG-TGCAATGGACTCAATTTC--G--ACTCGGCC---------------AACATCATCGAAAAACTAATTGATTGCAG--GCACCCCTCGAGTTGCCCCGCCTG
droKik1	scf7180000302367:9733-9936 -		GCTTAATTGAGCCC----CCACTAGGTCCATACCATTTCCATATATATATATATATATATATATATATATATCTATATATC----CGTGTAATTTGTG-------C----------T----GCGAG--TGTGTCAAAGTTGAG----------------CTGATAATGCGATCATCTTGT----------------------------------------ATAATGATCA-TGCAATGGACTCAATTTT-------TGGGC--------------------TCATCAAGAAACTAATTGATTGCAGCGGCAC-------------AGGCCCT
droAna3	scaffold_12929:3083392-3083624 +		ACTTAATTGATCG-----CCACAAGTTC------------------------------------------------ATATC----CGTGTAATTTGTG-GCTCTGCCCGCTTTTCTTGTCAAA-AGT-TGTGTCAAAGTTGAG----------------TCGATAATGCGATCATT-TGTGGCAAAGTGGCAGGCAGAGCCTATAGTGTGGCCTAATGGCATAATGATCATTGCAATGGACTCAATTTTTTG--AGTCTGGA--GTCTTTTTTGGAAAAAA----AAA-ATACTAATTGATTGCAA--GCACCTC-----------CGATCA
droBip1	scf7180000396037:18940-19150 +		ATTTAATTGATCG-----CCACAAGTTC------------------------------------------------ATATC----CGTGTAATTTGTG-G-----CCCGTCCAAAAAGCT----GG--TGTGTCAAAGTTGAG----------------TCGATATAGCGATCATT-TGTGGCAAAG-------------------TGTGACCTAATGTCATAATGATCATTGCAATGGACTCCATTTTCAAAGACTCGGTAAAGAAAAAATCAGAAAAAA----AAT-ATACTAATTGATAGCAA--GCACCTCTG--ATCACC-------
dp5	XL_group1a:315380-315545 +		CACTAATTGCTCTCCTCTCCACAAGTTC------------------------------------------------ATGTCGCTCGGAGTAAT-----------------------C----GC-AGTGTGTGTCAAAGTTGAG----------------ATGATAATGCGATCATCTTGT----------------------------------------ATAATGATCG-TGCAATGGATTCGACTGT--G--GTCCG-CGCCGAA----------GACCTCATCAA-AACCTAATTGATTG-----------------TTTTCCTACCTT
droPer2	scaffold_26:351631-351796 -		CACTAATTGCTCTCCTCTCCACAAGCTC------------------------------------------------ATGTCGCTCGGAGTAAT-----------------------C----GC-AGTGTGTGTCAAAGTTGAG----------------ATGATAATGCGATCATCTTGT----------------------------------------ATAATGATCG-TGCAATGGATTCGACTTT--G--GTCCG-CGCCGAA----------GACCTCATCAA-AACCTAATTGATTG-----------------TTTTCCTACCTT

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droSim3

ATTTAATTGATCC-----GCACAAGTTC------------------------------------------------ATATC----CGTGTAATTTGTG-------CCCGTCTCTTTT----GC-AG--TGTGTCAAAGTTGAG----------------TCGATAATGCGATCATCTTGT----------------------------------------ATAATGATCG-TGCAATGGACTCAATTTC--G--ACTCGGCA---------------AACATCATCAA-AGCCTAATTGATTGCAG--GCACCCCTCGAATTGCCCCGCCTT	Size	Hit Count	Total Norm	Total	V044 Embryo	M023 Head	M024 Male-body	M025 Embryo	O001 Testis	O002 Head	M053 Female-body	SRR553485 Ovary	SRR553486 Ovary	SRR553487 Ovary	SRR553488 Ovary	SRR618934 Ovary
...................................................................................................................................................................................................................................TCG-TGCAATGGACTCAATTTC--G--..............................................................................	22	1	73.00	73	1	35	10	9	0	0	0	8	6	0	4	0
......................................................................................................................................AAAGTTGAG----------------TCGATAATGCGAT................................................................................................................................................................	22	1	37.00	37	2	7	1	4	0	0	0	0	5	3	4	11
......................................................................................................................................AAAGTTGAG----------------TCGATAATGCGA.................................................................................................................................................................	21	1	26.00	26	1	6	3	0	0	2	0	4	7	1	2	0
...................................................................................................................................................................................................................................TCG-TGCAATGGACTCAATTTC--.................................................................................	21	1	12.00	12	0	7	4	0	0	1	0	0	0	0	0	0
......................................................................................................................................AAAGTTGAG----------------TCGATAATGCGATC...............................................................................................................................................................	23	1	4.00	4	0	2	0	0	1	1	0	0	0	0	0	0
......................................................................................................................................AAAGTTGAG----------------TCGATAATGC...................................................................................................................................................................	19	1	4.00	4	0	1	0	0	1	2	0	0	0	0	0	0
....................................................................................................................................................................................................................................CG-TGCAATGGACTCAATTTC--G--A.............................................................................	22	1	3.00	3	1	1	0	1	0	0	0	0	0	0	0	0
.........................................................................................................................................GTTGAG----------------TCGATAATGCGATC...............................................................................................................................................................	20	1	3.00	3	0	2	0	0	0	0	0	0	0	0	0	1
......................................................................................................................................AAAGTTGAG----------------TCGATAATGCG..................................................................................................................................................................	20	1	3.00	3	0	2	0	0	0	1	0	0	0	0	0	0
....................................................................................................................................................................................................................................CG-TGCAATGGACTCAATTTC--G--..............................................................................	21	1	2.00	2	0	2	0	0	0	0	0	0	0	0	0	0
...................................................................................................................................................................................................................................TCG-TGCAATGGACTCAAT......................................................................................	18	1	2.00	2	0	1	0	0	1	0	0	0	0	0	0	0
.......................................................................................................................................AAGTTGAG----------------TCGATAATGCGATC...............................................................................................................................................................	22	1	2.00	2	0	1	0	0	0	0	1	0	0	0	0	0
...................................................................................................................................................................................................................................TCG-TGCAATGGACTCAATTTC--G--A.............................................................................	23	1	1.00	1	0	0	0	0	0	0	0	0	0	0	1	0
.................................................................................................................................................................................................................................GATCG-TGCAATGGACTCAAT......................................................................................	20	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
....................................................................................................................T----GC-AG--TGTGTCAAAGTTGAG----------------T............................................................................................................................................................................	21	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0
....................................................................................................................................................................................................................................CG-TGCAATGGACTCAATTTC--.................................................................................	20	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0
.........................................................................................................................................GTTGAG----------------TCGATAATGCGATCA..............................................................................................................................................................	21	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0
..................................................................................................................................................................................................................................ATCG-TGCAATGGACTCAATTTC--G--..............................................................................	23	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0
..................................................................................................................................................................................................................................ATCG-TGCAATGGACTCAATTT....................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	1	0	0	0
..........................................................................................................................................TTGAG----------------TCGATAATGCGAT................................................................................................................................................................	18	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	1
.........................................................................................................................................GTTGAG----------------TCGATAATGCGA.................................................................................................................................................................	18	1	1.00	1	0	0	0	0	0	1	0	0	0	0	0	0
..................................................................................................................................................................................................................................ATCG-TGCAATGGACTCAATTTC--.................................................................................	22	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0

droSec2

ATTTAATTGATCC-----GCACAAGTTC------------------------------------------------ATATC----CGTGTAATTTGTG-------CCCGTCTCTTTT----GC-AG--TGTGTCAAAGTTGAG----------------TCGATAATGCGATCATCTTGT----------------------------------------ATAATGATCG-TGCAATGGACTCAATTTC--G--ACTCGGCA---------------AACATCATCAA-AGCCTAATTGATTGCAG--GCACCCCTCGAATTGCCCCGCCTT	Size	Hit Count	Total Norm	Total	V113 Male-body	V114 Embryo	V115 Head	M054 Female-body
......................................................................................................................................AAAGTTGAG----------------TCGATAATGCGA.................................................................................................................................................................	21	1	25.00	25	9	3	11	2
......................................................................................................................................AAAGTTGAG----------------TCGATAATGCGAT................................................................................................................................................................	22	1	18.00	18	1	7	4	6
...................................................................................................................................................................................................................................TCG-TGCAATGGACTCAATTTC--G--..............................................................................	22	1	12.00	12	1	2	5	4
................................................................................................................................................................................................................................TGATCG-TGCAATGGACTCAATT.....................................................................................	22	1	3.00	3	0	1	2	0
.........................................................................................................................................GTTGAG----------------TCGATAATGCGATCA..............................................................................................................................................................	21	1	3.00	3	2	0	1	0
.......................................................................................................................................AAGTTGAG----------------TCGATAATGCGATC...............................................................................................................................................................	22	1	2.00	2	1	0	1	0
......................................................................................................................................AAAGTTGAG----------------TCGATAATGCG..................................................................................................................................................................	20	1	2.00	2	0	0	2	0
.........................................................................................................................................GTTGAG----------------TCGATAATGCGATCAT.............................................................................................................................................................	22	1	1.00	1	0	1	0	0
...........................................................................................ATTTGTG-------CCCGTCTCTTTT----GC-................................................................................................................................................................................................................	21	1	1.00	1	0	1	0	0
.......................................................................................................................................AAGTTGAG----------------TCGATAATGCGAT................................................................................................................................................................	21	1	1.00	1	0	0	0	1
................................................................................................................................................................................................................................TGATCG-TGCAATGGACTCAAT......................................................................................	21	1	1.00	1	0	0	1	0
........................................................................................................................................AGTTGAG----------------TCGATAATGCGATCA..............................................................................................................................................................	22	1	1.00	1	0	1	0	0

droEre2

ATTTAATTGATCC-----GCACAAGTTC------------------------------------------------ATATC----CGTGTAATTTGTGCG-----CCAGTCTCTTTT----GC-AG--TGTGTCAAAGTTGAG----------------TCGATAATGCGATCATCTTGT----------------------------------------ATAATGATCG-TGCAATGGACTCAATTTC--G--ACTCGGCA---------------AACATCATCAA-AACCTAATTGATTGCAG--GCACCCTTCAAATTGCCCCGCCTT	Size	Hit Count	Total Norm	Total	V040 Embryo	V060 Head	V107 Male-body	V108 Head	M055 Female-body	M057 Embryo
......................................................................................................................................AAAGTTGAG----------------TCGATAATGCGA.................................................................................................................................................................	21	1	20.00	20	0	1	6	11	2	0
......................................................................................................................................AAAGTTGAG----------------TCGATAATGCGAT................................................................................................................................................................	22	1	18.00	18	0	1	7	9	0	1
.........................................................................................................................................GTTGAG----------------TCGATAATGCGATCAT.............................................................................................................................................................	22	1	10.00	10	0	1	5	4	0	0
.........................................................................................................................................GTTGAG----------------TCGATAATGCGATCA..............................................................................................................................................................	21	1	5.00	5	0	0	1	3	1	0
...................................................................................................................................................................................................................................TCG-TGCAATGGACTCAATTTC--G--..............................................................................	22	1	5.00	5	0	0	1	4	0	0
................................................................................................................................................................................................................................TGATCG-TGCAATGGACTCAATT.....................................................................................	22	1	2.00	2	0	0	1	1	0	0
.......................................................................................................................................AAGTTGAG----------------TCGATAATGCGATC...............................................................................................................................................................	22	1	1.00	1	0	0	0	0	1	0
...................................................................................................................................................................................................................................TCG-TGCAATGGACTCAATTTC--.................................................................................	21	1	1.00	1	0	0	0	1	0	0
..................................................................................................................................................................................................................................ATCG-TGCAATGGACTCAATTT....................................................................................	21	1	1.00	1	0	0	0	1	0	0
....................................................................................................................................................................................................................................CG-TGCAATGGACTCAATTTC--G--A.............................................................................	22	1	1.00	1	0	0	0	1	0	0
.......................................................................................................................................AAGTTGAG----------------TCGATAATGCGATCA..............................................................................................................................................................	23	1	1.00	1	0	0	1	0	0	0
......................................................................................................................................AAAGTTGAG----------------TCGATAATGCG..................................................................................................................................................................	20	1	1.00	1	0	0	1	0	0	0

droYak3

ATTTAATTGATCC-----GCACAAGTTC------------------------------------------------ATATC----CGTGTAATTTGTG-------CCAGTCTCTTTT----GC-AG--TGTGTCAAAGTTGAG----------------ACGATAATGCGATCATCTTGT----------------------------------------ATAATGATCG-TGCAATGGACTCAATTTC--G--ACTCGGCA---------------AACATCATCAA-AACCTAATTGATTGCAG--GCACCCCTTAAATTGCCCCGCCCT	Size	Hit Count	Total Norm	Total	V046 Embryo	V052 Head	V120 Male-body	V058 Head	M026 Head	M043 Female-body	M056 Embryo
.............................................................................................................................................................................................................................................GGACTCAATTTC--G--ACTCGGC.......................................................................	20	1	2.00	2	0	0	0	0	2	0	0
........................GTTC------------------------------------------------ATATC----CGTGTAATTTGTG-------CCA................................................................................................................................................................................................................................	25	1	1.00	1	0	0	1	0	0	0	0
............................................................................................................................AG--TGTGTCAAAGTTGAG----------------AC...........................................................................................................................................................................	19	1	1.00	1	0	0	0	0	0	0	1

droEug1

ATTTAATTGATCC-----GCACAAGTTC------------------------------------------------ATATC----CGTGTAATTTGTG-------CCCGTCTCTTTT----GC-AGC-TGTGTCAAAGTTGAG----------------TTGATAATGCGATCATCTTGT----------------------------------------ATAATGATCA-TGCAATGAACTCAATTTC--A--ACTCGGCA---------------AACATCATCAAAAAACTAATTGATTGCAG--GCACCCCTCAAATTGCCCCGCCTG	Size	Hit Count	Total Norm	Total
No Reads

droBia1

ACCTAATTGATCC-----GCACAAGTTC------------------------------------------------ATATC----CGTGTAAT-TGCG-------CCCGTCTCCCCG----GC-AG--GGC-CCAGAGCTGAGGGCATTGGCTGTGGCGTCGATAACGCGATCATCTTGT----------------------------------------ATGGTGATCG-TGCAATGGACTCAATTCC-----CCTCGGCA---------------GACATCATCGG-AAACTAATTGATGGCGG--GCACCCCTCGCTCGGCCCCGCCCG	Size	Hit Count	Total Norm	Total
No Reads

droTak1

ATTTAAGTGATCC-----GCACAAGTTC------------------------------------------------ATATC----CGTGTAATTTGCG-------CCCCTCTCTTTT----GC-AG--TGTGTCAAAGTTGAG----------------TTGATAATGCGATCATCTTGT----------------------------------------ATAATGATCG-TGCAATGGACTCAATTTC--G--AACCGGCA---------------AACATCATCAA-AAACTAATTGATTGCAG--GCAAGCCTCAAATTGCCCCGCCTT	Size	Hit Count	Total Norm	Total
No Reads

droEle1

ATTTAATTGATCC-----GCACAAGTTC------------------------------------------------ATATC----CGTGTAATTTGTG-------CCAGTCTCTTTC----GC-AG--TGTGTCAAAGTTGAG----------------TTGATAATGCGATCATCTTGT----------------------------------------ATAATGATCG-TGCAATGGACTCAATTTC--G--TCTCGGCA---------------AACATCATCAA-AAACTAATTGATTGCAG--GCACCCCTCAAATTGCCCCGCCTG	Size	Hit Count	Total Norm	Total
No Reads

droRho1

ATTTAATTGATCC-----GCACAAGTTC------------------------------------------------ATATC----CGTGTAATTTGTG-------CCAGTCTCTTTT----GC-AG--TATGTCAAAGTTAAG----------------TTGATAATGCGATCATCTTGT----------------------------------------ATAATGATCG-TGCAATGGACTCAATTTC--G--ACTCGGCA---------------AACATCATCAA-AAACTAATTGATTGTAG--GCACCCCTCAAATTGCCCCGCCTG	Size	Hit Count	Total Norm	Total
No Reads

droFic1

ATTTAATTGATCC-----GCACAAGTTC------------------------------------------------ATATC----CGTGTAATTTGTG-------CCCGTCTCTTTC----GC-AG--TGTGTCAAAGTTGAG----------------TCGATAATGCGATCATCTTGT----------------------------------------ATAATGATCG-TGCAATGGACTCAATTTC--G--ACTCGGCC---------------AACATCATCGAAAAACTAATTGATTGCAG--GCACCCCTCGAGTTGCCCCGCCTG	Size	Hit Count	Total Norm	Total
No Reads

droKik1

GCTTAATTGAGCCC----CCACTAGGTCCATACCATTTCCATATATATATATATATATATATATATATATATCTATATATC----CGTGTAATTTGTG-------C----------T----GCGAG--TGTGTCAAAGTTGAG----------------CTGATAATGCGATCATCTTGT----------------------------------------ATAATGATCA-TGCAATGGACTCAATTTT-------TGGGC--------------------TCATCAAGAAACTAATTGATTGCAGCGGCAC-------------AGGCCCT	Size	Hit Count	Total Norm	Total
No Reads

droAna3

ACTTAATTGATCG-----CCACAAGTTC------------------------------------------------ATATC----CGTGTAATTTGTG-GCTCTGCCCGCTTTTCTTGTCAAA-AGT-TGTGTCAAAGTTGAG----------------TCGATAATGCGATCATT-TGTGGCAAAGTGGCAGGCAGAGCCTATAGTGTGGCCTAATGGCATAATGATCATTGCAATGGACTCAATTTTTTG--AGTCTGGA--GTCTTTTTTGGAAAAAA----AAA-ATACTAATTGATTGCAA--GCACCTC-----------CGATCA	Size	Hit Count	Total Norm	Total	V105 Male-body	V106 Head	V055 Head	V039 Embryo	M044 Female-body	M058 Embryo
..............................................................................................................................................................................................................................AATGATCATTGCAATGGA............................................................................................	18	1	1.00	1	0	0	0	1	0	0
..........................................................................................AATTTGTG-GCTCTGCCCGCTTTTCTTGTC....................................................................................................................................................................................................................	29	1	1.00	1	0	0	0	0	1	0

droBip1

ATTTAATTGATCG-----CCACAAGTTC------------------------------------------------ATATC----CGTGTAATTTGTG-G-----CCCGTCCAAAAAGCT----GG--TGTGTCAAAGTTGAG----------------TCGATATAGCGATCATT-TGTGGCAAAG-------------------TGTGACCTAATGTCATAATGATCATTGCAATGGACTCCATTTTCAAAGACTCGGTAAAGAAAAAATCAGAAAAAA----AAT-ATACTAATTGATAGCAA--GCACCTCTG--ATCACC-------	Size	Hit Count	Total Norm	Total
No Reads

dp5

CACTAATTGCTCTCCTCTCCACAAGTTC------------------------------------------------ATGTCGCTCGGAGTAAT-----------------------C----GC-AGTGTGTGTCAAAGTTGAG----------------ATGATAATGCGATCATCTTGT----------------------------------------ATAATGATCG-TGCAATGGATTCGACTGT--G--GTCCG-CGCCGAA----------GACCTCATCAA-AACCTAATTGATTG-----------------TTTTCCTACCTT	Size	Hit Count	Total Norm	Total	V112 Male-body	V043 Embryo	V051 Head	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head
No Reads

droPer2

CACTAATTGCTCTCCTCTCCACAAGCTC------------------------------------------------ATGTCGCTCGGAGTAAT-----------------------C----GC-AGTGTGTGTCAAAGTTGAG----------------ATGATAATGCGATCATCTTGT----------------------------------------ATAATGATCG-TGCAATGGATTCGACTTT--G--GTCCG-CGCCGAA----------GACCTCATCAA-AACCTAATTGATTG-----------------TTTTCCTACCTT	Size	Hit Count	Total Norm	Total	V111 Male-body	V042 Embryo	V050 Head	V057 Head	M021 Embryo	M042 Female-body
No Reads

dsi-mir-2583

X:5118687-5118768 +

Known

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-31.8	-31.5	-31.5