ID:

dsi-mir-2581

 Coordinate:

chrXh_Mrandom_005:3780-3852 +

 Confidence:

Known

 Type:

Unknown

 [View on miRBase]
[View on UCSC Genome Browser {Cornell Mirror}]
Legend: mature star mismatch in alignment mismatch in read

Predicted structure

-37.0 -36.9

Flybase annnotation

intergenic

No Repeatable elements found

Sense Strand Reads

ATTTGTCTGCACTAATAAGTTTTTCCCTTGATACAAATATTTATATATCTGTTGAACCGAAGACACTAGAATAACTAGTTACAAGTTATAGTTAACTAGTTGTTCTAGTGTCTTTGTTTGAACCACATCGTTTTCTTTTTTTTTCATTCTGCAAAATGAGTTTTATTCAGTGT
***********************************...............(((.((.(((((((((((((((((((((((((........)).))))))))))))))))))))))).)).)))...............*********************************** 		Read size # Mismatch Hit Count Total Norm Total SRR553485
Ovary		 GSM343915
embryo
 SRR553487
Ovary		 SRR618934
Ovary		 SRR553488
Ovary		 SRR553486
Ovary		 M025
embryo
 M053
Female-body
....................................................TGAACCGAAGACACTAGAATAACTAGT.............................................................................................. 27 0 1 5.00 5 1 0 0 2 1 1 0 0
.........................................................CGAAGACACTAGAATAACTAGT.............................................................................................. 22 0 1 3.00 3 0 3 0 0 0 0 0 0
.................................................TGTTGAACCGAAGACACTAGAATAAC.................................................................................................. 26 0 1 2.00 2 0 0 0 0 1 0 0 1
...........................................................................TAGTTACAAGTTATAGTTAACTAGT......................................................................... 25 0 1 2.00 2 0 0 0 2 0 0 0 0
...........................................TATATCTGTTGAACCGAAGACACTAGAA...................................................................................................... 28 0 1 2.00 2 1 0 0 0 1 0 0 0
.........................................................CGAAGACACTAGAATAACTAGTT............................................................................................. 23 0 1 2.00 2 0 2 0 0 0 0 0 0
.................................................TGTTGAACCGAAGACACTAGAAT..................................................................................................... 23 0 1 1.00 1 1 0 0 0 0 0 0 0
.........................................................CGAAGACACTAGAATTACTAGT.............................................................................................. 22 1 1 1.00 1 0 1 0 0 0 0 0 0
........................................TTATATATCTGTTGAACCGAAGACA............................................................................................................ 25 0 1 1.00 1 1 0 0 0 0 0 0 0
.......................................TTTATATATCTGTTGAACCGAAGACAC........................................................................................................... 27 0 1 1.00 1 0 0 0 0 0 0 1 0
.....................................TATTTATATATCTGTTGAACCGAA................................................................................................................ 24 0 1 1.00 1 1 0 0 0 0 0 0 0
...................................................TTGAACCGAAGACACTAGAATAACTAGT.............................................................................................. 28 0 1 1.00 1 1 0 0 0 0 0 0 0
....................................................AGAACCGAAGACACTAGAATAACTAGT.............................................................................................. 27 1 1 1.00 1 1 0 0 0 0 0 0 0
.......................................TTTATATATCTGTTGAACCGAAGA.............................................................................................................. 24 0 1 1.00 1 0 0 1 0 0 0 0 0
..TTGTCTGCACTAATAAGTTTTTCCCT................................................................................................................................................. 26 0 1 1.00 1 0 0 1 0 0 0 0 0
.......................................TTTATATATCTGTTGAACCGAATACAC........................................................................................................... 27 1 1 1.00 1 0 0 0 0 0 0 1 0
.........................................................................................................TAGTGTCTTTGTTTGAACCACATCGTT......................................... 27 0 1 1.00 1 0 0 0 0 0 1 0 0
.........................................TATATATCTGTTGAACCGAAGACACTAGA....................................................................................................... 29 0 1 1.00 1 0 0 1 0 0 0 0 0
....................................................................................................TGTTCTAGTGTCTTTGTTTGAACCACA.............................................. 27 0 1 1.00 1 0 0 1 0 0 0 0 0
..........................................................................................................AGTCTCTTTGTTCGTACCA................................................ 19 3 19 0.05 1 0 0 1 0 0 0 0 0
.....................................................................................................GTTCGAGTCTCTTTGTT....................................................... 17 2 20 0.05 1 0 0 0 0 0 1 0 0
......TTGCACTAATAACTTGTTCC................................................................................................................................................... 20 3 20 0.05 1 0 0 1 0 0 0 0 0

Anti-sense strand reads

ACACTGAATAAAACTCATTTTGCAGAATGAAAAAAAAAGAAAACGATGTGGTTCAAACAAAGACACTAGAACAACTAGTTAACTATAACTTGTAACTAGTTATTCTAGTGTCTTCGGTTCAACAGATATATAAATATTTGTATCAAGGGAAAAACTTATTAGTGCAGACAAAT
***********************************...............(((.((.(((((((((((((((((((((((.((........))))))))))))))))))))))))).)).)))...............*********************************** 		Read size # Mismatch Hit Count Total Norm Total SRR553485
Ovary		 M025
embryo
 M053
Female-body		 SRR553487
Ovary		 SRR618934
Ovary
....................................................................................................TATTCTAGTGTCTTCGGTTCAACAGA............................................... 26 0 1 2.00 2 2 0 0 0 0
.........................................................CAAAGACACTAGAACAACTAGT.............................................................................................. 22 0 1 1.00 1 1 0 0 0 0
...........................TGAAAAAAAAAGAAAACGATGTGGT......................................................................................................................... 25 0 1 1.00 1 0 0 0 0 1
.......................................................AACAAAGACACTAGAACAACT................................................................................................. 21 0 1 1.00 1 0 1 0 0 0
....................TGCAGAATGAAAAAAAAAGAAAACGA............................................................................................................................... 26 0 1 1.00 1 0 0 0 1 0
....................................................TCAAACAAAGACACTAGAACAACTAGTT............................................................................................. 28 0 1 1.00 1 0 0 1 0 0
................................................................................................TAGTTATTCTAGTGTCTTTGGTTCAAC.................................................. 27 1 4 0.25 1 0 0 0 0 0
...................................................TTCAACCAAAGACACTAGAAC..................................................................................................... 21 1 5 0.20 1 0 0 0 0 0
.......................................................AACAAAGACACTAGAAAAACT................................................................................................. 21 1 6 0.17 1 1 0 0 0 0
...................TTGCAGAATGTAAAAAAAAA...................................................................................................................................... 20 2 16 0.06 1 0 0 0 0 0

Re-alignment of all predicted orthologs

Species Coordinate ID Alignment
droSim3 chrXh_Mrandom_005:3730-3902 + dsi-mir-2581 ATT-TGTCTGCACTAATAAGTTTTTCCCTTGATACAAATATTTATATATCTGTTGAACCGAAGACACTAGAATAACTAGTTACA---------AGTTATAGTTAACTAGTTGTTCTAGTGTCTTTGTTTGAACCACATCGTTTTCTTTTTTTTTCATTCTGCAAAATGAGTTTTATTCAGTGT
droSec2 scaffold_109:22174-22351 + ----TGTCTGCAATAATAAGTTTTTCCATTGATACAAATATTTATATATCTTTTGAACCAAAGACAGTAGAATTACTAGTTACTAGTTATACTAGTTATAGTTAACTAGTTATTCTAGTGTCTTTGTTTGAACCATATCGTTTT--TTTTTTTTCATTCTAAAAAATGAGTTTTATTCAGTGT
dm3 chrXHet:74898-74997 - ATTTTGTCTGCAACGATAAGTTTTTCCCTTGATACAAATATTTATATATCTTTTGAGC---------------------------------------------------------------------------CATAGCGT--------TTTTTCATTCTGCAAAATTAGTTTTATTCAATGT
droEre2 scaffold_4845:20881752-20881836 + CA-------------------------------------------------------------------------------------------GGTAACAGGTATCTGGTAGTCCGGGAA-------CTCGACCATAGCATTCTATCTTGTTTTCATTCTGCCAATAGCAATTTAATCAGTGT
droYak3 v2_chrUn_1329:26346-26446 + AAT-TATCTGCAACGATAAGTTTTTCCTCTGTTACAAATATTTTAATATCTTTTGAACCAAA---------------------------------------------------------------------------GCGT------TTTTTTTCATTCTGCAAAATGAGTTTTATTCAGCGT
droBip1 scf7180000395874:7173-7286 + ACG-CGACTGCAATATTAAATTTGCACATT-----AAATATTTGCATATTAATT-TGCAATATATATTAAAATAAAAAGCTAA------------------------------------------------------ACAA--------TATTACATTTTTTGAAATGAATTCCAATCTGTGT

Re-alignment of all predicted orthologs with sRNA reads

Species Read alignment
droSim3
ATT-TGTCTGCACTAATAAGTTTTTCCCTTGATACAAATATTTATATATCTGTTGAACCGAAGACACTAGAATAACTAGTTACA---------AGTTATAGTTAACTAGTTGTTCTAGTGTCTTTGTTTGAACCACATCGTTTTCTTTTTTTTTCATTCTGCAAAATGAGTTTTATTCAGTGTSizeHit CountTotal NormTotalV044
Embryo		M023
Head		M024
Male-body		M025
Embryo		O001
Testis		O002
Head		M053
Female-body		SRR553485
Ovary		SRR553486
Ovary		SRR553487
Ovary		SRR553488
Ovary		SRR618934
Ovary
.....................................................TGAACCGAAGACACTAGAATAACTAGT.......................................................................................................2715.005000000011012
..........................................................CGAAGACACTAGAATAACTAGT.......................................................................................................2213.003300000000000
..................................................TGTTGAACCGAAGACACTAGAATAAC...........................................................................................................2612.002000000100010
............................................................................TAGTTACA---------AGTTATAGTTAACTAGT.........................................................................2512.002000000000002
............................................TATATCTGTTGAACCGAAGACACTAGAA...............................................................................................................2812.002000000010010
..........................................................CGAAGACACTAGAATAACTAGTT......................................................................................................2312.002200000000000
..................................................TGTTGAACCGAAGACACTAGAAT..............................................................................................................2311.001000000010000
.........................................TTATATATCTGTTGAACCGAAGACA.....................................................................................................................2511.001000000010000
........................................TTTATATATCTGTTGAACCGAAGACAC....................................................................................................................2711.001000100000000
......................................TATTTATATATCTGTTGAACCGAA.........................................................................................................................2411.001000000010000
....................................................TTGAACCGAAGACACTAGAATAACTAGT.......................................................................................................2811.001000000010000
........................................TTTATATATCTGTTGAACCGAAGA.......................................................................................................................2411.001000000000100
..T-TGTCTGCACTAATAAGTTTTTCCCT..........................................................................................................................................................2611.001000000000100
...................................................................................................................TAGTGTCTTTGTTTGAACCACATCGTT.........................................2711.001000000001000
..........................................TATATATCTGTTGAACCGAAGACACTAGA................................................................................................................2911.001000000000100
..............................................................................................................TGTTCTAGTGTCTTTGTTTGAACCACA..............................................2711.001000000000100
droSec2
----TGTCTGCAATAATAAGTTTTTCCATTGATACAAATATTTATATATCTTTTGAACCAAAGACAGTAGAATTACTAGTTACTAGTTATACTAGTTATAGTTAACTAGTTATTCTAGTGTCTTTGTTTGAACCATATCGTTTT--TTTTTTTTCATTCTAAAAAATGAGTTTTATTCAGTGTSizeHit CountTotal NormTotalV113
Male-body		V114
Embryo		V115
Head		M054
Female-body
.............................................................................................................GTTATTCTAGTGTCTTTGT.......................................................19200.0510010
droEre2
CA-------------------------------------------------------------------------------------------GGTAACAGGTATCTGGTAGTCCGGGAA-------CTCGACCATAGCATTCTATCTTGTTTTCATTCTGCCAATAGCAATTTAATCAGTGTSizeHit CountTotal NormTotalV040
Embryo		V060
Head		V107
Male-body		V108
Head		M055
Female-body		M057
Embryo
No Reads
droYak3
AAT-TATCTGCAACGATAAGTTTTTCCTCTGTTACAAATATTTTAATATCTTTTGAACCAAA---------------------------------------------------------------------------GCGT------TTTTTTTCATTCTGCAAAATGAGTTTTATTCAGCGTSizeHit CountTotal NormTotalV046
Embryo		V052
Head		V120
Male-body		V058
Head		M026
Head		M043
Female-body		M056
Embryo
...........................................TAATATCTTTTGAACCAAA---------------------------------------------------------------------------GCGT------T...................................2430.3310010000
...........................................TAATATCTTTTGAACCAAA---------------------------------------------------------------------------GCGT------TTTT................................2730.3310000001
..........................................................................................................................................................CATTCTGCAAAATGAGTTTTATTCAG...2630.3310000010
droBip1
ACG-CGACTGCAATATTAAATTTGCACATT-----AAATATTTGCATATTAATT-TGCAATATATATTAAAATAAAAAGCTAA------------------------------------------------------ACAA--------TATTACATTTTTTGAAATGAATTCCAATCTGTGTSizeHit CountTotal NormTotal
No Reads

Generated: 12/06/2013 at 02:41 PM