dsi_9431

Antisense to intron [2l_1117861_1117914_+]; Antisense to five_prime_UTR [2l_1117679_1117730_+]; Antisense to CDS [2l_1117731_1117860_+]; Antisense to exon [2l_1117679_1117860_+]

TTTGAAAATTGAGCTTTTCGGGGATTTCGTGCACCAAAAGCCTTACCCTCGTAACGCTTCAAATAGTTGCTCTTTGCAATTGCGTGTGCAATGCCACCATTGTGCAATTTCCTCACATTCAGATCGTTTTTCATAAAGCAACAGCAGTAGTCGCAATAATAACTTTTGCCACCCATTTAAATCTAAACAATAAATACAATACTTTCAAGCGTAGAGATGGCAAATGTGCTCGATAGTTTTCGGGAGCATA ***************************************************************************************..(((((....)).)))...................(((((....)))))....((((.(((..........))).))))...............****************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	SRR902009 testis	O001 Testis	SRR553486 Makindu_3 day-old ovaries	GSM343915 embryo	SRR553485 Chicharo_3 day-old ovaries	M023 head
...........................................................................................................TTTCCTCACATTCCGTCCGTT..........................................................................................................................	21	3	3	3.33	10	0	0	10	0	0	0
.............................................................................................................................................................................................................CAAGCGTAGAGATGGCAATT.........................	20	1	1	1.00	1	1	0	0	0	0	0
.........................................................................................AATGCCACCATTGTGCAATTTC...........................................................................................................................................	22	0	1	1.00	1	0	1	0	0	0	0
.............................................................................................................TCCTCACATTCAGATCGTTTT........................................................................................................................	21	0	1	1.00	1	1	0	0	0	0	0
..............................................................................................................................................................................................................AAGCATAGGGATGGCAAAT.........................	19	2	1	1.00	1	0	0	0	1	0	0
..........................................................................................................ATTTCCTCACATTCAGATCGT...........................................................................................................................	21	0	1	1.00	1	1	0	0	0	0	0
..........................................................................................................ATTCATTCACATTCAGATCGTTTT........................................................................................................................	24	3	1	1.00	1	1	0	0	0	0	0
...................................................................................................................................................................TTTTGCCACCCATTTAAATCT..................................................................	21	0	1	1.00	1	0	1	0	0	0	0
...............................CACCAAAAGCCTTACCCTCGT......................................................................................................................................................................................................	21	0	1	1.00	1	0	1	0	0	0	0
.............................................................................................................TCCTCACGTTCAGATCGTTTT........................................................................................................................	21	1	1	1.00	1	1	0	0	0	0	0
................................................................................................................TCACATTCAGATCGTTTTTC......................................................................................................................	20	0	1	1.00	1	1	0	0	0	0	0
...........................................................................................................TTTCCTCACATTCAGATCGTT..........................................................................................................................	21	0	1	1.00	1	1	0	0	0	0	0
....................................................................................TGTGCAATGCCACCATTGTGC.................................................................................................................................................	21	0	1	1.00	1	0	1	0	0	0	0
...........................................................................................................................................................................................................TTCAAGCGTAGAGATGGCAAAT.........................	22	0	1	1.00	1	1	0	0	0	0	0
................................................................................................................................................................................................AATACAATACTTTCAAGCGTA.....................................	21	0	1	1.00	1	0	1	0	0	0	0
............................................................................................................TTCCTCACATTCCGTCCGTTT.........................................................................................................................	21	3	4	0.75	3	0	0	3	0	0	0
...........................................................................................................TTTCCTCACATTCCGTCCGTTT.........................................................................................................................	22	3	2	0.50	1	0	0	1	0	0	0
...........................................................................................................TTTCCTCACATTCCGTCCGT...........................................................................................................................	20	3	11	0.18	2	0	0	2	0	0	0
.........................................................................................................................................................................................................CTTTCGAGCGTAGAG..................................	15	1	6	0.17	1	0	0	1	0	0	0
...............................................................................................................................................TCAGTAGTCGCATTAATA.........................................................................................	18	2	8	0.13	1	0	0	0	0	1	0
....................................................................................................................................................................................................CAATCCTGTCAAGCTTAGA...................................	19	3	12	0.08	1	0	0	0	1	0	0
........................................................................................................................................................................................................................................ATAGTGTTCGGAAGCATG	18	3	20	0.05	1	0	0	0	0	0	1

----------------------------------------------######################################################################################################################################################################################---------------------- AAACTTTTAACTCGAAAAGCCCCTAAAGCACGTGGTTTTCGGAATGGGAGCATTGCGAAGTTTATCAACGAGAAACGTTAACGCACACGTTACGGTGGTAACACGTTAAAGGAGTGTAAGTCTAGCAAAAAGTATTTCGTTGTCGTCATCAGCGTTATTATTGAAAACGGTGGGTAAATTTAGATTTGTTATTTATGTTATGAAAGTTCGCATCTCTACCGTTTACACGAGCTATCAAAAGCCCTCGTAT ****************************************************************..(((((....)).)))...................(((((....)))))....((((.(((..........))).))))...............***************************************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	SRR553486 Makindu_3 day-old ovaries	O001 Testis	SRR618934 dsim w501 ovaries	SRR902009 testis	M023 head	SRR553487 NRT_0-2 hours eggs	M025 embryo
...........................................................................................................AAAGGAGTGTAAGGCAGGCAA..........................................................................................................................	21	3	3	1.33	4	4	0	0	0	0	0	0
............................................................................................................AAGGAGTGTAAGGCAGGCAAA.........................................................................................................................	21	3	4	1.00	4	4	0	0	0	0	0	0
.................................................................................................................GTGTAAGTCTAGCAAAAAGTA....................................................................................................................	21	0	1	1.00	1	0	1	0	0	0	0	0
..........................................AATGGGGGCGTTGCGAAGTTTG..........................................................................................................................................................................................	22	3	1	1.00	1	0	0	0	1	0	0	0
..............................................................................................................GGAGTGGAAGTCTGGCAAA.........................................................................................................................	19	2	1	1.00	1	0	0	1	0	0	0	0
.......................................................................................................................................................................CGGTGGGTAAATTTAGATTT...............................................................	20	0	1	1.00	1	0	1	0	0	0	0	0
.....................................................................................................................................................CAGCGTTATTATTGAAAA...................................................................................	18	0	1	1.00	1	0	0	0	0	1	0	0
...............................................................................................TGGTAACACGTTAAAGGAGTGTAAGTCT...............................................................................................................................	28	0	1	1.00	1	0	0	0	0	0	1	0
................................................................................................................................................................................................................CGCATCTCTACCGTTTAC........................	18	0	1	1.00	1	0	1	0	0	0	0	0
............................................................................................................AAGGAGTGTAAGGCAGGCAA..........................................................................................................................	20	3	9	0.78	7	7	0	0	0	0	0	0
...........................................................................................................AAAGGAGTGTAAGGCAGGCAAA.........................................................................................................................	22	3	2	0.50	1	1	0	0	0	0	0	0
....................................................................................................ACACGTTAAAGGAGT.......................................................................................................................................	15	0	3	0.33	1	0	0	1	0	0	0	0
..............................TATGGTTTTCGGAATGGGA.........................................................................................................................................................................................................	19	2	3	0.33	1	0	0	0	1	0	0	0
.......................................................................GGAACGTTAACGCACAGGTTT..............................................................................................................................................................	21	3	6	0.17	1	0	0	0	0	0	0	1
..................................................................................................................................AGAATTTCCTTGTCCTCATC....................................................................................................	20	3	8	0.13	1	1	0	0	0	0	0	0
...............................................................................................................................................CGTCCTCAGCGTGATTA..........................................................................................	17	2	20	0.05	1	0	0	1	0	0	0	0

Species	Coordinate	ID	Alignment
droSim2	2l:1117657-1117906 -	dsi_9431	TTTGA----AA---ATTGAGCT---------TTT-------CGGGG-------------------------ATTTCGTGC-----ACCAAAA-GC--CTTACCCTCGTAACGCTTCAAATAGTTGCTCTTTGCAATTGCGTGTGCAATGCCACCATTGTGCAATTTCCTCACATTCAGATCGTTTTTCATAAAGCAACAGCAGTAGTCGCAATAATAACTTTTGCCACCCATTT-----------------------------------AAAT--------CTAAA----------CAATA-A----------------ATACAATACTTTCAAGCGTAGAGATGGCAAATG------------TGCTC---------------------GATAGTTTTCGGGAGCATA
droSec2	scaffold_14:1130245-1130495 -		TTTGA----AA---ATTGAGCT---------TT------TTCGGGG-------------------------ATTTCGTGC-----ACCAAAA-GC--CTTACCCTCGTAACGCTTCAAAAAGTTGCTCTTTGCAATTGCGTGTGCAATGCCACCATTGTGCAATTTCCTCACATTCAGATCGTTTTTCATAAAGCAACAGCAGTAGTCGCAATAATAACTTTTGCCACCCATAT-----------------------------------AAAT--------CTAAT----------CAATA-A----------------ATACAATACTTTCAAGCGTAGAGATGACAAATG------------TGCTC---------------------GATAGTTTTCGGGAGCATA
dm3	chr2L:1170148-1170410 -		ATTGA----AA---ATTCAGCT---------TT------TTCGGGA-------------------------ATATCGTGC-----AACAAAA-GC--TTTACCCTCGTAACGCTTCAAATAGTTGCTCTTTGCAATTGCGTGTGCAATACCACCATTGTGCAATTTCCTCACATTCAGATCGTTTTTCAGAAAGCAACAGCAGTAGTCGCAATAATAACTTTTGCCACCCATTT-----------------------------------AAAT--------GTAAA----------CAAAATA----------------ATACAATACTTTTAAGCGTAGAGATGGCAAATGTTAT-CAAGCCGTTCTC---------------------GATAGTTTTCCGGAGCATC
droEre2	scaffold_4929:1214816-1215086 -		TTAAA----AAAAAATTAAGGT---------TT------TTGTAGC-------------------------ATATCTTGA-----ACCAAAT-AG--CTTACTCTCGTAACGCTTCATATAATTGGTCTTGGCGATTGTGTGTGAAATGCCATCATTGTGCAATTTCCTCACATTCAGATCGTTTTTCATAAAGCAGCAGCAGTAGTCGCAATAATAACTTTTTCCACCCATTA-CTTTT-----------------------------AAAT--------GTAAA----------CAAAACA----------------ATTCATTACTTTTAAGCATAGAGAAGGCAAATGGGAT-CAAGCCGTTCTC---------------------GATAGTTTTCTTGAATATC
droYak3	2L:1149815-1150083 -		TTTAA----AA---ATTAGATCT--------GTT-----TTGTTGG-------------------------ATATCGTGC-----ACCAAAA-AG--CTTACTCTCGTAACGCTTCATATAATTGGCCTTTGCTATTGTGTGTGAAATACCATCATTGTGCAATTTCCTTACATTCAGATCGTTTTTCATATAGCAACAGCAGTAGTCGCAATAATAACTTTTTCCCCCCATTG-CTGCT-----------------------------AAAT--------GTAAA----------CAAAATA----------------AAACAATACTTTTAAGCGTAGAGATGGCAAATGGAAT-ACAACCTTTCTC---------------------GATAGGTTTCTAG-GTATT
droEug1	scf7180000409005:36467-36748 +		TTTCG----AT---TTTTTGGTA-------AAA--------------------ATACTTGTTTA-TGG---AGTTC----------AGATTG-TC--CTTACCCTCATACCGTTTCATATAGTTGATCCTTGCAATAGTGTGGGAGAGACCTACATTGTGCAATTTTCTCACATTAACATCGTTTTTTAAGAAACAACAGCAGTAATCGCAATAATAACTTTTCCCACCCATTT-CATGA-----------------------------AAAT--------GTAAACAAACTTTGTT-TAACA----------------AAGCAAGACTTTCAGGCCTAGAGATGACAGTCAAAAATCAAAT--TGAACGATAGAGT-------------------CCTTATATTTATC
droBia1	scf7180000302188:684293-684540 +		TTTAA----AT---ATTAAATTT--------AT--------GC------------TTTGTGT-------------TGTTT-----ACAAAGG-TT--CTTACCCTCATAACGTCTCATATAGTTGGTCTTGGCAATTGTGTGAGAAATGCCGCCATTGTGCAATTTGCGCACATTTAAATCGTTTTTCATAAAACAACAACAGTAGTCGCAATAATAACTTTTTCCACCCATTT-TT-AG-----------------------------AAAT--------GTAAA----------CAAAACAATATGCTAACCATAAAATACAATACTTTTAAGCGTAGAGATGGCAAATCG--------------------------------------------------------
droTak1	scf7180000415264:189960-190236 +		TTAGA----AA---ATAT-------------------------------------ATTTTTTTA-AAG-----A----CT-----GTAAAAC-TT--GTTACCCTCATAACGCTTCATATAGTTGGTTTTTGCAATTGTGTGGGAAATACCACTATTGTGCAATTTGCGCACATTTAAATCGTTTTTCATAAAACAACAGCAGTAATCGCAATAGTAACTTTTTCCACCCATTT-CATAA-----------------------------AAAT--------GTAAA----------CAAAAGA----------------ATTAAATACTTTTTGGCATAGGGATGGCAAACTTGAT-AATAATGTTGCT--GATGACGTTTTTTAATATCGATA--TTTCG-AAGTATA
droEle1	scf7180000491273:2309189-2309428 +		TTTTT----AA---TTTTAAATT--------ATGAAGTTTTAGTGT-------------------------AG--AGTGT-----GGAGAAA-TA--ATTACCGTCATAACGGCGCATATAATTTGTTTTCGCAATTGTGTGGGAGATGCCATCATTGTGCAGTTTGCGCACATTTAAATCGTTTTTCATAAAACAACAACAATAATCACAATAATAACTTTTTCCACCCATTTGCCAAA-----------------------------AAGT--------GTAAA----------CAATGCA----------------AAACAATACTTTTGAACGTAGAGATGGTAAACCG--------------------------------------------------------
droRho1	scf7180000776759:27054-27314 -		TTTCG----AA---TTTTAAATT--------ATT-------TATT-------------TAGT------------TATTGA-AAAT-GGTAAA-TC--CTTACCATCATAACGCCGCATATAATTTGTTTTTGCAATTGTGTGGGAGATGCCATCATTGTGCAGTTTTCGCACATTTAAATCGTTTTTCATAAAACAACAACAGTAATCACAAAAGTAACTTTTTCCGCCCATTG-CT-AA-----------------------------AAAT--------ATAAA----------CAACTCA----------------AAACAATACTTTTTAGCATAGAGATGGAAGACAGGATTCAAGCCTG-------------------------------TTTCGGCAGAATG
droFic1	scf7180000454155:386560-386809 -		TTCAA----TA---CTAATAA----------AT--------------------ATACCCATTTT-----------TAACA-----ATGAGAA-GT--TTTACCTTCAAATCGCCTCATGTAATTCGTTTTTGCAATTGTGTGAGATATTCCATCGTTGTGCAGTTTTCTTACATTTAAGTCGTTTTTCATAAAACAACAACAGTAGTCACAGTAGTAACTCTTTCCACCCATTA-TTTAAAGTCAAACGTAATCGGACAAGAGTTAACAAAAT--------GTAAA----------CAGCGCA----------------AAACAAAACTTTGAGGGATAAA--------------------------------------------------------------------
droKik1	scf7180000302405:415273-415527 -		TTTAA----CA---TTTAAATCTCTGAATA--------------------------------TA-TGAATC---------TAAAT-GG-T-G-CA--TTTACCGTCGAATCTCCGCATATAATTGGCCCTTGCAATTGTGTGGGCAATGCCATTATTGTGAAGTTTTCGTACGTTCAAGTCGTTTTTCATGAAACAGCAACAATAATCGCAGTAGTAACTTTTGCCACCCATTG-CTAAA----------------------------------CAAAAAAGTAAA----------CAAAACA----------------AA-----------------AGAGATGACAATCAAGGTTGTTAT--TGAGCGATAGGAC-------------------TTTGTTAATTATC
droAna3	scaffold_12916:4643436-4643629 -		TTTAG----AA---GGCAGGCC---------T----------------TGA--------------------ATATTTTCC-----GGGGAAG-TC--CCTACCTTTCAAGCGCAGCACATAATTGGCTTTGCAAATTGTGTGGGCGATGCCACTGTTGTGCAGTTTCCGCACATTTATATCGTTTTTCATGAAACAACAACAATAGTCGCAAAAATAGCTCTTTCCACCCATTT-CCTTC------------------------------------------CAAT----------T-------------------------------------------------------------------G-------------------------------TTTCG----AATA
droBip1	scf7180000396737:121304-121538 +		TTTAG----TT---GGCAGCCT------------------CATAGG-------------------------AT--TTTAA-----GGGGAAG-TC--CCTACCTGTTAGGCGTCGCAAATAATTGGTTTTGCAAATCGTGTGGGCGATGCCACTATTGTGCAATTTGCGTACATTTAAGTCATTTTTCATAAAACAACAACAATAATCGCAAAAATAGCTCTTTCCGCCCATTG-TTTTA-----------------------------A-----TAAAATCTAAA----------CAAT--------------------------AATTATTTAATTAGAGATGGTA-------------------CC---------------------AACAAATATCG---GTATA
dp5	Unknown_group_557:2733-2975 -		GCCAA----AC---AGTGGGTT---------AGT-------TGGACAGTGGGTACGCTCTGTTG-CAAGCA---------GAGAT-AG-TAG-CT--CGTACCTTCAAAGCGCCGCATGTACCGATACTTGGCCACCGTGTGAGATATGCCATTGTTGTGCAGCTTCCGCACATTCAAGTCACTTTTCATAAAACAACCGCAGTAATCACAAAAATAACTTTGCC---CCATTT-GG-AA-----------------------------AGGGGTGAAAATGTAAA----------CAAT-------------------------AACTTTTCGTCCTAGAGATGGTTAA-----------------------------------------------------------
droPer2	scaffold_8694:352-594 +		GTCAA----AC---AGTGGGTT---------AGT-------TGGACAGTGGGTACGCTCTGTTG-CAAGCA---------GAGAT-AG-TAG-CT--CGTACCTTCAAAGCGCCGCATGTACCGATACTTGGCCACCGTGTGAGATATGCCATTGTTGTGCAGCTTCCGCACATTCAAGTCACTTTTCATAAAACAACCGCAGTAATCACAAAAATAACTTTGCC---CCATTT-GG-AA-----------------------------AGGGGTGAAAATGTAAA----------CAAT-------------------------AACTTTTCGTCCTAGAGATGGTTAA-----------------------------------------------------------
droWil2	scf2_1100000004521:2769112-2769321 -		GTTTA----CA---ATATATGT-----ACATATG------TAT-T-------------TTAT--------------TTGT-----GGGAAGT-CC--CTTACTTTCGAATCTTTGCATGTAGGTCACCTTGGCTGCTGTATGCAGCATGCCAGCGTTGTGCAATTTACGCACATTAATATCATTTTTCATAAAACAACAACAATAATCGCAATAATAACTTTTGCCACCCATTT-TTTTC-----------------------------AGAAATTA-------------------------------------------------------------AAAAATAACAAATATAA------------------------------------------------------
droVir3	scaffold_12963:7100389-7100584 -		ATACGTTAATA----TT--ATT---------TA------TTAT-----------------GCA--------TTTAAATGC-----AAGCGCT-AG--CTCACCCTCAAAGCGACGCAGGTAGGTTGCCTTCGCAGCGGTGTGCGAAGCACCCGCATTGTGCAGCTTGCGCACATTTAGGTCATTTTTCATATAGCAACAGCAGTAATCACAATAATAGCTTTTGCCGCCCATTT-AC----------------------------------AA--------ATAAA----------AAAAA-A----------------A-----------------------------------------------------------------------------------------
droMoj3	scaffold_6500:3824408-3824601 +		AAAAA----TAGAATTTAAT-AA--------AT--------AT------------TTTTTGCT---------------TA-----ACTAAATATCCCAGTACCTTCAAATCGACGCATATAGGTTGCCTTGGCAGCCGTGTGTGAAATGCCTGCATTGTGTAATTTACGCACGTTTAGATCATTTTTCATAAAGCAACAACAGTAATCACAATAATAGCTTTTTCCACCCATTT-AG-TA-----------------------------AATT--------ACAAA---------------------------------------------------------------------------------------------------------------------------
droGri2	scaffold_15252:10249183-10249370 -		ATTAT----AA---ATTATTTT---------T---------------------------AGCTTAA-----AGATAAACAA----AACTAA--AG--CCCACCCTCAAATTGCCGCATGTAAGTTGCTTTTGCAGCTGTGTGCGAAATGCCCGAATTGTGTAATTTGCGCACATTCAAATCGTTCTTCATGAAGCAACAGCAGTAATCACAGTAATAACTTTTGCCACCCATTA-TT----------------------------------AA--------CTAAA----------C----------------------------------------------------------------------------------------------------------------

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droSim2

TTTGA----AA---ATTGAGCT---------TTT-------CGGGG-------------------------ATTTCGTGC-----ACCAAAA-GC--CTTACCCTCGTAACGCTTCAAATAGTTGCTCTTTGCAATTGCGTGTGCAATGCCACCATTGTGCAATTTCCTCACATTCAGATCGTTTTTCATAAAGCAACAGCAGTAGTCGCAATAATAACTTTTGCCACCCATTT-----------------------------------AAAT--------CTAAA----------CAATA-A----------------ATACAATACTTTCAAGCGTAGAGATGGCAAATG------------TGCTC---------------------GATAGTTTTCGGGAGCATA	Size	Hit Count	Total Norm	Total	M023 Head	M024 Male-body	M025 Embryo	M053 Female-body	O001 Testis	O002 Head	SRR553485 Ovary	SRR553486 Ovary	SRR553487 Ovary	SRR553488 Ovary	SRR618934 Ovary	V044 Embryo	SRR902008 Ovary	SRR902009 Testis	SRR1275487 Male larvae	SRR1275485 Male prepupae	SRR1275483 Male prepupae
.................................................................................................................................................AATGCCACCATTGTGCAATTTC..................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0
.....................................................................................................................................................................TCCTCACATTCAGATCGTTTT...............................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
..................................................................................................................................................................ATTTCCTCACATTCAGATCGT..................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
...........................................................................................................................................................................................................................TTTTGCCACCCATTT-----------------------------------AAAT--------CT..............................................................................................................................	21	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0
...............................................................................C-----ACCAAAA-GC--CTTACCCTCGT.............................................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0
........................................................................................................................................................................TCACATTCAGATCGTTTTTC.............................................................................................................................................................................................................................	20	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
...................................................................................................................................................................TTTCCTCACATTCAGATCGTT.................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
............................................................................................................................................TGTGCAATGCCACCATTGTGC........................................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0
.........................................................................................................................................................................................................................................................................................................................................TTCAAGCGTAGAGATGGCAAAT..........................................................	22	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
..............................................................................................................................................................................................................................................................................................................A----------------ATACAATACTTTCAAGCGTA......................................................................	21	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0

droSec2

TTTGA----AA---ATTGAGCT---------TT------TTCGGGG-------------------------ATTTCGTGC-----ACCAAAA-GC--CTTACCCTCGTAACGCTTCAAAAAGTTGCTCTTTGCAATTGCGTGTGCAATGCCACCATTGTGCAATTTCCTCACATTCAGATCGTTTTTCATAAAGCAACAGCAGTAGTCGCAATAATAACTTTTGCCACCCATAT-----------------------------------AAAT--------CTAAT----------CAATA-A----------------ATACAATACTTTCAAGCGTAGAGATGACAAATG------------TGCTC---------------------GATAGTTTTCGGGAGCATA	Size	Hit Count	Total Norm	Total	M054 Female-body	V113 Male-body	V114 Embryo	V115 Head
No Reads

dm3

ATTGA----AA---ATTCAGCT---------TT------TTCGGGA-------------------------ATATCGTGC-----AACAAAA-GC--TTTACCCTCGTAACGCTTCAAATAGTTGCTCTTTGCAATTGCGTGTGCAATACCACCATTGTGCAATTTCCTCACATTCAGATCGTTTTTCAGAAAGCAACAGCAGTAGTCGCAATAATAACTTTTGCCACCCATTT-----------------------------------AAAT--------GTAAA----------CAAAATA----------------ATACAATACTTTTAAGCGTAGAGATGGCAAATGTTAT-CAAGCCGTTCTC---------------------GATAGTTTTCCGGAGCATC	Size	Hit Count	Total Norm	Total	GSM154618 Ovary	GSM154620 Ovary	GSM154621 Ovary	GSM154622 Ovary	GSM180328 Head	GSM180329 Mixed-body	GSM180330 Embryo	GSM180331 Embryo	GSM180332 Embryo	GSM180333 Embryo	GSM180334 Larvae	GSM180335 Disc	GSM180336 Pupae	GSM180337 S2	GSM231091 Ovary	GSM239041 Head	GSM239050 Head	GSM239051 S2	GSM239052 S2	GSM246084 Head	GSM266765 S2	GSM280082 Ovary	GSM280083 Ovary	GSM280084 Ovary	GSM280085 Testis	GSM280086 Ovary	GSM280087 S2-NP	GSM280088 S2-NP	GSM312995 Head	GSM313160 Head	GSM313161 Head	GSM313162 Head	GSM313163 Head	GSM313164 Head	GSM313165 Head	GSM343287 Embryo	GSM379050 Ovary	GSM379051 Ovary	GSM379052 Ovary	GSM379053 Ovary	GSM379054 Ovary	GSM379055 Ovary	GSM379056 Ovary	GSM379057 Ovary	GSM379058 Ovary	GSM379059 Ovary	GSM379060 Ovary	GSM379061 Ovary	GSM379062 Ovary	GSM379063 Ovary	GSM379064 Ovary	GSM379065 Ovary	GSM379066 Ovary	GSM379067 Ovary	GSM424739 S2	GSM424740 S2	GSM424741 S2	GSM467729 Female-body	GSM467730 Female-body	GSM467731 Female-body	M002 CS	M003 Male-body	M004 Male-body	M005 ML-DmD32	M006 ML-DmD32	M029 OSC	M030 OSC	M031 OSC	M032 S2-R+	M033 S2-R+	M034 S2-R+	M035 S2-R+	M036 S2-R+	M037 S2-R+	M038 S2-R+	M039 S2-R+	M048 S2-R+	OSS2 OSS	OSS6 OSS	OSS7 OSS	OSS8 OSS	S1 Head	S10 Embryo	S11 Embryo	S12 Embryo	S13 Embryo	S14 Embryo	S15 S2-GMR	S16 Kc	S17 Larvae	S18 Larvae	S19 Pupae	S2 Head	S20 Pupae	S21 Disc	S22 Head	S23 Female-body	S24 Male-body	S25 Larvae	S26 Larvae	S27 Pupae	S28 Pupae	S29 Head	S3 Male-body	S30 S2-GMR	S31 S2	S32 S2	S33 Kc	S34 Kc	S35 Kc	S4 Female-body	S5 Disc	S6 Embryo	S7 Embryo	S8 S2	S9 Embryo	SRR001337 Head	SRR001338 Head	SRR001339 Head	SRR001340 Head	SRR001341 Head	SRR001342 Head	SRR001343 Head	SRR001344 Head	SRR001345 Head	SRR001346 Head	SRR001347 Head	SRR001348 Head	SRR001349 Head	SRR001664 Head	SRR010951 Ovary	SRR010952 Ovary	SRR010953 Ovary	SRR010954 Ovary	SRR010955 Ovary	SRR010956 Ovary	SRR010957 Ovary	SRR010958 Ovary	SRR010959 Ovary	SRR010960 Ovary	SRR014268 Embryo	SRR014269 Embryo	SRR014270 Embryo	SRR014271 Embryo	SRR014272 Embryo	SRR014273 Ovary	SRR014274 Ovary	SRR014275 Ovary	SRR014276 Ovary	SRR014277 Ovary	SRR014278 Ovary	SRR014279 Ovary	SRR014280 Ovary	SRR014281 Ovary	SRR014282 Ovary	SRR023197 S2	SRR023399 S2	SRR023400 S2	SRR023402 S2	SRR023407 S2	SRR029028 S2	SRR029029 S2	SRR029030 S2	SRR029031 S2	SRR029032 S2	SRR029033 S2	SRR029608 Head	SRR029633 Head	SRR030145 gb3	SRR031692 Head	SRR031693 Head	SRR031694 Head	SRR031696 Head	SRR031697 Head	SRR031698 Head	SRR031699 Head	SRR031700 Head	SRR031701 Head	SRR031702 Head	SRR031703 Head	SRR031704 Head	SRR032092 S2	SRR032093 S2	SRR032094 S2	SRR032095 S2-NP	SRR032096 S2-NP	SRR032152 Head	SRR060643 Testis	SRR060644 Ovary	SRR060645 Testis	SRR060646 Ovary	SRR060647 Ovary	SRR060648 Ovary	SRR060649 Ovary	SRR060650 Testis	SRR060651 Ovary	SRR060652 Testis	SRR060653 Ovary	SRR065151 Ovary	SRR065152 Ovary	SRR065153 Ovary	SRR065154 Ovary	SRR065155 Ovary	SRR065800 Ovary	SRR065801 Ovary	SRR065802 Ovary	SRR065803 Ovary	SRR065804 Ovary	SRR065805 Ovary	SRR065806 Ovary	SRR065807 Ovary	SRR097865 S2-NP	SRR097866 S2-NP	SRR097867 S2-NP	SRR298536 Ovary	SRR298537 Ovary	SRR298711 Ovary	SRR298712 Ovary	SRR317110 Ovary	SRR317111 Ovary	SRR317112 Ovary	SRR317113 Ovary	SRR317114 Ovary	SRR317115 Ovary	SRR317116 Ovary	SRR317117 Ovary	SRR341115 S2	SRR341116 S2	SRR341117 S2	SRR341118 S2	SRR351332 Mixed-body	SRR351333 Mixed-body	T004 OSC	T005 CME-L1	V001 Embryo	V002 Embryo	V003 S2	V006 Female-body	V007 Head	V008 S2	V009 CME-L1	V010 ML-DmD20-C5	V011 Sg4	V012 Male-body	V013 Head	V014 DTT	V015 DreRFHV	V018 Kc167	V019 GM2	V020 S2-R+	V021 ML-DmD21	V022 ML-DmD32	V023 Head	V024 Kc167	V025 S2-R+	V026 1182-2H	V027 ML-DmD21	V028 CME-W1-Cl.8+	V029 ML-DmD9	V030 ML-DmD8	V031 GM2	V032 S1	V033 CME-W1-Cl.8+	V034 ML-DmD16-C3	V035 ML-DmD32	V036 ML-DmD20-C5	V037 S2	V038 S2	V063 Ovary	V0632 Ovary	V064 Ovary	V0642 Ovary	V065 Ovary	V0652 Ovary	V066 Ovary	V0662 Ovary	V067 S2-NP	V073 mbn2	V074 S3	V075 ML-DmBG1-C1	V076 ML-DmBG3-C2	V077 Head	V078 Head	V079 Head	V080 Head	V081 Embryo	V082 Embryo	V083 Male-body	V084 Female-body	V085 CME-W2	V086 Female-body	V088 Ovary	V0882 Ovary	V089 Ovary	V0892 Ovary	V090 Male-body	V091 fGS/OSS	V092 Male-body	V093 Male-body	V094 S2-R+	V095 S2-R+	V096 Ovary	V097 Ovary	V098 Ovary	V125 ML-DmD9	V126 CME-L1	V127 G2	V128 S3	V129 ML-DmBG1-C1	V130 ML-DmBG3-C2	V131 ML-DmD16-C3	V132 ML-DmD32	V133 Sg4	V134 ML-DmD8	V135 CME-W2	V136 Male-body	V137 Head	V138 Male-body	V139 Female-body	V140 Female-body	V141 Female-body	V142 Female-body	V144 OSC	V145 S2	V146 S1	V147 1182-2H	V148 mbn2	GSM1528798 follicle cells
...............................TT------TTCGGGA-------------------------ATATCGTGC-----AAC.................................................................................................................................................................................................................................................................................................................................	21	1	6.00	6	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	6	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
............................................................................................................................GCTCTTTGCAATTGCGTGTGC........................................................................................................................................................................................................................................................................	21	1	4.00	4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..................................................................................................................................................................ATTTCCTCACATTCAGATCGT..................................................................................................................................................................................................................................	21	1	2.00	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0
..........................................................................................................................................................................................TCAGAAAGCAACAGCAGTAGT..........................................................................................................................................................................................................	21	1	2.00	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
............................................................................................................................................................................................................................................................................................................................................AAGCGTAGAGATGGCAAATGT........................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..........................................................................................................................TTGCTCTTTGCAATTGCGTGT..........................................................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
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.............................................................................................................................................................................TTCAGATCGTTTTTCAGAAAG.......................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...............................TT------TTCGGGA-------------------------ATATCGTGC-----AACA................................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
......................................................................................................................................................................................TTTTTCAGAAAGCAACAGCAG..............................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.............................................................................................................................................................................TTCAGATCGTTTTTCAGAAAGCAAC...................................................................................................................................................................................................................	25	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...............................................................................................................................CTTTGCAATTGCGTGTGCAAT.....................................................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0
....................................................................................................................................................................TTCCTCACATTCAGATCGTTT................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

droEre2

TTAAA----AAAAAATTAAGGT---------TT------TTGTAGC-------------------------ATATCTTGA-----ACCAAAT-AG--CTTACTCTCGTAACGCTTCATATAATTGGTCTTGGCGATTGTGTGTGAAATGCCATCATTGTGCAATTTCCTCACATTCAGATCGTTTTTCATAAAGCAGCAGCAGTAGTCGCAATAATAACTTTTTCCACCCATTA-CTTTT-----------------------------AAAT--------GTAAA----------CAAAACA----------------ATTCATTACTTTTAAGCATAGAGAAGGCAAATGGGAT-CAAGCCGTTCTC---------------------GATAGTTTTCTTGAATATC	Size	Hit Count	Total Norm	Total	M055 Female-body	M057 Embryo	V060 Head	V040 Embryo	V107 Male-body	V108 Head	GSM1528801 follicle cells
No Reads

droYak3

TTTAA----AA---ATTAGATCT--------GTT-----TTGTTGG-------------------------ATATCGTGC-----ACCAAAA-AG--CTTACTCTCGTAACGCTTCATATAATTGGCCTTTGCTATTGTGTGTGAAATACCATCATTGTGCAATTTCCTTACATTCAGATCGTTTTTCATATAGCAACAGCAGTAGTCGCAATAATAACTTTTTCCCCCCATTG-CTGCT-----------------------------AAAT--------GTAAA----------CAAAATA----------------AAACAATACTTTTAAGCGTAGAGATGGCAAATGGAAT-ACAACCTTTCTC---------------------GATAGGTTTCTAG-GTATT

Size

Hit Count

Total Norm

Total

M026
Head

M043
Female-body

M056
Embryo

V046
Embryo

V052
Head

V058
Head

V120
Male-body

SRR1275488
Male larvae

SRR1275486
Male prepupae

SRR1275484
Male prepupae

GSM1528802
follicle cells

No Reads

droEug1

TTTCG----AT---TTTTTGGTA-------AAA--------------------ATACTTGTTTA-TGG---AGTTC----------AGATTG-TC--CTTACCCTCATACCGTTTCATATAGTTGATCCTTGCAATAGTGTGGGAGAGACCTACATTGTGCAATTTTCTCACATTAACATCGTTTTTTAAGAAACAACAGCAGTAATCGCAATAATAACTTTTCCCACCCATTT-CATGA-----------------------------AAAT--------GTAAACAAACTTTGTT-TAACA----------------AAGCAAGACTTTCAGGCCTAGAGATGACAGTCAAAAATCAAAT--TGAACGATAGAGT-------------------CCTTATATTTATC	Size	Hit Count	Total Norm	Total
No Reads

droBia1

TTTAA----AT---ATTAAATTT--------AT--------GC------------TTTGTGT-------------TGTTT-----ACAAAGG-TT--CTTACCCTCATAACGTCTCATATAGTTGGTCTTGGCAATTGTGTGAGAAATGCCGCCATTGTGCAATTTGCGCACATTTAAATCGTTTTTCATAAAACAACAACAGTAGTCGCAATAATAACTTTTTCCACCCATTT-TT-AG-----------------------------AAAT--------GTAAA----------CAAAACAATATGCTAACCATAAAATACAATACTTTTAAGCGTAGAGATGGCAAATCG--------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droTak1

TTAGA----AA---ATAT-------------------------------------ATTTTTTTA-AAG-----A----CT-----GTAAAAC-TT--GTTACCCTCATAACGCTTCATATAGTTGGTTTTTGCAATTGTGTGGGAAATACCACTATTGTGCAATTTGCGCACATTTAAATCGTTTTTCATAAAACAACAGCAGTAATCGCAATAGTAACTTTTTCCACCCATTT-CATAA-----------------------------AAAT--------GTAAA----------CAAAAGA----------------ATTAAATACTTTTTGGCATAGGGATGGCAAACTTGAT-AATAATGTTGCT--GATGACGTTTTTTAATATCGATA--TTTCG-AAGTATA	Size	Hit Count	Total Norm	Total
No Reads

droEle1

TTTTT----AA---TTTTAAATT--------ATGAAGTTTTAGTGT-------------------------AG--AGTGT-----GGAGAAA-TA--ATTACCGTCATAACGGCGCATATAATTTGTTTTCGCAATTGTGTGGGAGATGCCATCATTGTGCAGTTTGCGCACATTTAAATCGTTTTTCATAAAACAACAACAATAATCACAATAATAACTTTTTCCACCCATTTGCCAAA-----------------------------AAGT--------GTAAA----------CAATGCA----------------AAACAATACTTTTGAACGTAGAGATGGTAAACCG--------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droRho1

TTTCG----AA---TTTTAAATT--------ATT-------TATT-------------TAGT------------TATTGA-AAAT-GGTAAA-TC--CTTACCATCATAACGCCGCATATAATTTGTTTTTGCAATTGTGTGGGAGATGCCATCATTGTGCAGTTTTCGCACATTTAAATCGTTTTTCATAAAACAACAACAGTAATCACAAAAGTAACTTTTTCCGCCCATTG-CT-AA-----------------------------AAAT--------ATAAA----------CAACTCA----------------AAACAATACTTTTTAGCATAGAGATGGAAGACAGGATTCAAGCCTG-------------------------------TTTCGGCAGAATG	Size	Hit Count	Total Norm	Total
No Reads

droFic1

TTCAA----TA---CTAATAA----------AT--------------------ATACCCATTTT-----------TAACA-----ATGAGAA-GT--TTTACCTTCAAATCGCCTCATGTAATTCGTTTTTGCAATTGTGTGAGATATTCCATCGTTGTGCAGTTTTCTTACATTTAAGTCGTTTTTCATAAAACAACAACAGTAGTCACAGTAGTAACTCTTTCCACCCATTA-TTTAAAGTCAAACGTAATCGGACAAGAGTTAACAAAAT--------GTAAA----------CAGCGCA----------------AAACAAAACTTTGAGGGATAAA--------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droKik1

TTTAA----CA---TTTAAATCTCTGAATA--------------------------------TA-TGAATC---------TAAAT-GG-T-G-CA--TTTACCGTCGAATCTCCGCATATAATTGGCCCTTGCAATTGTGTGGGCAATGCCATTATTGTGAAGTTTTCGTACGTTCAAGTCGTTTTTCATGAAACAGCAACAATAATCGCAGTAGTAACTTTTGCCACCCATTG-CTAAA----------------------------------CAAAAAAGTAAA----------CAAAACA----------------AA-----------------AGAGATGACAATCAAGGTTGTTAT--TGAGCGATAGGAC-------------------TTTGTTAATTATC	Size	Hit Count	Total Norm	Total
No Reads

droAna3

TTTAG----AA---GGCAGGCC---------T----------------TGA--------------------ATATTTTCC-----GGGGAAG-TC--CCTACCTTTCAAGCGCAGCACATAATTGGCTTTGCAAATTGTGTGGGCGATGCCACTGTTGTGCAGTTTCCGCACATTTATATCGTTTTTCATGAAACAACAACAATAGTCGCAAAAATAGCTCTTTCCACCCATTT-CCTTC------------------------------------------CAAT----------T-------------------------------------------------------------------G-------------------------------TTTCG----AATA	Size	Hit Count	Total Norm	Total	M044 Female-body	M058 Embryo	V039 Embryo	V055 Head	V105 Male-body	V106 Head
................................................................................................................................TTGCAAATTGTGTGGGCGATG....................................................................................................................................................................................................................................................................	21	1	1.00	1	1	0	0	0	0	0

droBip1

TTTAG----TT---GGCAGCCT------------------CATAGG-------------------------AT--TTTAA-----GGGGAAG-TC--CCTACCTGTTAGGCGTCGCAAATAATTGGTTTTGCAAATCGTGTGGGCGATGCCACTATTGTGCAATTTGCGTACATTTAAGTCATTTTTCATAAAACAACAACAATAATCGCAAAAATAGCTCTTTCCGCCCATTG-TTTTA-----------------------------A-----TAAAATCTAAA----------CAAT--------------------------AATTATTTAATTAGAGATGGTA-------------------CC---------------------AACAAATATCG---GTATA	Size	Hit Count	Total Norm	Total
No Reads

dp5

GCCAA----AC---AGTGGGTT---------AGT-------TGGACAGTGGGTACGCTCTGTTG-CAAGCA---------GAGAT-AG-TAG-CT--CGTACCTTCAAAGCGCCGCATGTACCGATACTTGGCCACCGTGTGAGATATGCCATTGTTGTGCAGCTTCCGCACATTCAAGTCACTTTTCATAAAACAACCGCAGTAATCACAAAAATAACTTTGCC---CCATTT-GG-AA-----------------------------AGGGGTGAAAATGTAAA----------CAAT-------------------------AACTTTTCGTCCTAGAGATGGTTAA-----------------------------------------------------------	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
...........................................................TGTTG-CAAGCA---------GAGAT-AG-TAG-............................................................................................................................................................................................................................................................................................................................	21	2	5.00	10	0	2	0	0	0	0	0	0	8	0
.....................T---------AGT-------TGGACAGTGGGTACG.................................................................................................................................................................................................................................................................................................................................................................	19	2	2.50	5	0	0	0	0	0	0	0	0	5	0
...................................................................................................................CATGTACCGATACTTGGCCAC.................................................................................................................................................................................................................................................................................	21	2	2.50	5	0	5	0	0	0	0	0	0	0	0
..............................................................................................................................................................................................................TCACAAAAATAACTTTGCC---CC...................................................................................................................................................................................	21	2	1.50	3	0	3	0	0	0	0	0	0	0	0
..............................................................................................................CGCCGCATGTACCGATACTTG......................................................................................................................................................................................................................................................................................	21	2	1.50	3	0	3	0	0	0	0	0	0	0	0
.....................T---------AGT-------TGGACAGTGGGTACGCC...............................................................................................................................................................................................................................................................................................................................................................	21	2	1.50	3	0	0	0	0	0	0	0	0	3	0
....................................................TACGCTCTGTTG-CAAGCA---------G........................................................................................................................................................................................................................................................................................................................................	19	2	1.50	3	0	0	0	0	0	0	0	0	3	0
...........................................................................................G-CT--CGTACCTTCAAAGCGCCG......................................................................................................................................................................................................................................................................................................	21	2	1.50	3	0	2	0	0	0	0	1	0	0	0
...................GTT---------AGT-------TGGACAGTGGGTACG.................................................................................................................................................................................................................................................................................................................................................................	21	2	1.50	3	0	1	0	0	0	0	0	0	2	0
GCCAA----AC---AGTGGGTT---------AGT-------TGG.............................................................................................................................................................................................................................................................................................................................................................................	21	2	1.00	2	0	2	0	0	0	0	0	0	0	0
.................................T-------TGGACAGTGGGTACGCTCTG............................................................................................................................................................................................................................................................................................................................................................	21	2	1.00	2	0	1	0	0	0	0	1	0	0	0
...............................AGT-------TGGACAGTGGGTACGCTC..............................................................................................................................................................................................................................................................................................................................................................	21	2	1.00	2	0	1	0	0	0	0	0	0	1	0
.CCAA----AC---AGTGGGTT---------AGT-------TGG.............................................................................................................................................................................................................................................................................................................................................................................	20	2	1.00	2	0	0	0	0	0	0	0	0	2	0
..........................................................................................................................................................................................................................................................................................................AT-------------------------AACTTTTCGTCCTAGAGAT.................................................................	21	2	1.00	2	0	0	0	0	0	0	0	0	2	0
................TGGGTT---------AGT-------TGGACAGTGGG.....................................................................................................................................................................................................................................................................................................................................................................	20	2	1.00	2	0	2	0	0	0	0	0	0	0	0
............................................................................................................................................TGAGATATGCCATTGTTGTG.........................................................................................................................................................................................................................................................	20	2	1.00	2	0	1	0	0	0	0	1	0	0	0
..............................................................................................................................................AGATATGCCATTGTTGTG.........................................................................................................................................................................................................................................................	18	2	1.00	2	0	0	0	0	0	0	0	0	2	0
..............AGTGGGTT---------AGT-------TGGACAGTGG......................................................................................................................................................................................................................................................................................................................................................................	21	2	1.00	2	0	1	0	0	0	0	0	0	1	0
............................................................................................................................................TGAGATATGCCATTGTTGTGC........................................................................................................................................................................................................................................................	21	2	1.00	2	0	1	0	0	0	0	1	0	0	0
......................................................................A---------GAGAT-AG-TAG-CT--CGTACCTT................................................................................................................................................................................................................................................................................................................	21	2	1.00	2	0	2	0	0	0	0	0	0	0	0
.......................................................................................................................................................ATTGTTGTGCAGCTTCCGCA..............................................................................................................................................................................................................................................	20	2	1.00	2	0	2	0	0	0	0	0	0	0	0
..........................................................CTGTTG-CAAGCA---------GAGAT-AG-TA..............................................................................................................................................................................................................................................................................................................................	21	2	1.00	2	0	0	0	0	0	0	0	0	2	0
..........................................................................................................................CGATACTTGGCCACCGTGTGA..........................................................................................................................................................................................................................................................................	21	2	1.00	2	0	0	0	0	0	0	2	0	0	0
.................................................................................................................................TGGCCACCGTGTGAGATATG....................................................................................................................................................................................................................................................................	20	2	1.00	2	0	2	0	0	0	0	0	0	0	0
............................................................................................................................................................................................................AATCACAAAAATAACTTTGCC---.....................................................................................................................................................................................	21	2	1.00	2	0	2	0	0	0	0	0	0	0	0
................................................................................................................CCGCATGTACCGATACTTGGCC...................................................................................................................................................................................................................................................................................	22	2	1.00	2	0	2	0	0	0	0	0	0	0	0
...............GTGGGTT---------AGT-------TGGACAGTGGG.....................................................................................................................................................................................................................................................................................................................................................................	21	2	1.00	2	0	2	0	0	0	0	0	0	0	0
...........................................................................................................................GATACTTGGCCACCGTGTGAG.........................................................................................................................................................................................................................................................................	21	2	1.00	2	0	1	0	0	0	0	1	0	0	0
..................GGTT---------AGT-------TGGACAGTGGGTAC..................................................................................................................................................................................................................................................................................................................................................................	21	2	1.00	2	0	1	0	0	0	0	1	0	0	0
.........................................TGGACAGTGGGTACGCTCTG............................................................................................................................................................................................................................................................................................................................................................	20	2	1.00	2	0	1	0	0	0	0	0	0	1	0
......................................................................................................................................................CATTGTTGTGCAGCTTCCGC...............................................................................................................................................................................................................................................	20	2	1.00	2	0	1	0	0	0	0	1	0	0	0
.....................................................................................................................................................................................................................AATAACTTTGCC---CCATTT-GG-A..........................................................................................................................................................................	21	2	1.00	2	0	1	0	0	0	0	0	0	1	0
...............GTGGGTT---------AGT-------TGGACAGTGGGTACG.................................................................................................................................................................................................................................................................................................................................................................	25	2	0.50	1	0	0	0	0	0	0	0	0	1	0
......................................................................................................................................................................................................................................ATTT-GG-AA-----------------------------AGGGGTGAAAATG...............................................................................................................................	21	2	0.50	1	0	1	0	0	0	0	0	0	0	0
..CAA----AC---AGTGGGTT---------AGT-------TGGA............................................................................................................................................................................................................................................................................................................................................................................	20	2	0.50	1	0	0	0	0	0	0	0	0	1	0
................................................................................................................................TTGGCCACCGTGTGAGATATGT...................................................................................................................................................................................................................................................................	22	2	0.50	1	0	1	0	0	0	0	0	0	0	0
..............................................................................................................................................................................................................................................................................GGGGTGAAAATGTAAA----------CAAT-------------------------....................................................................................	20	2	0.50	1	0	1	0	0	0	0	0	0	0	0
....A----AC---AGTGGGTT---------AGT-------TGGACA..........................................................................................................................................................................................................................................................................................................................................................................	20	2	0.50	1	0	1	0	0	0	0	0	0	0	0
.........................................................................................................................................................................................................................ACTTTGCC---CCATTT-GG-AA-----------------------------AGG.........................................................................................................................................	21	2	0.50	1	0	1	0	0	0	0	0	0	0	0
.....................................................................................................................................................CCATTGTTGTGCAGCTTCCGC...............................................................................................................................................................................................................................................	21	2	0.50	1	0	1	0	0	0	0	0	0	0	0
............................................................................................................................................................................................................................TTGCC---CCATTT-GG-AA-----------------------------AGGGGTT.....................................................................................................................................	22	2	0.50	1	0	1	0	0	0	0	0	0	0	0
.....................................................................................................................................CACCGTGTGAGATATGCCATT...............................................................................................................................................................................................................................................................	21	2	0.50	1	0	1	0	0	0	0	0	0	0	0
...................................................................................................................................................................................................................AAAATAACTTTGCC---CCATTC-..............................................................................................................................................................................	20	2	0.50	1	0	0	0	0	0	0	0	0	1	0
...........................................................TGTTG-CAAGCA---------GAGAT-AG-TA..............................................................................................................................................................................................................................................................................................................................	20	2	0.50	1	0	0	0	0	0	0	0	0	1	0
..........................................................................................................................................................................................................................CTTTGCC---CCATTT-GG-AA-----------------------------AGGG........................................................................................................................................	21	2	0.50	1	0	0	0	0	0	0	1	0	0	0
.....................................................................................................................................................CCATTGTTGTGCAGCTTCC.................................................................................................................................................................................................................................................	19	2	0.50	1	0	1	0	0	0	0	0	0	0	0
.................................................................................................................CGCATGTACCGATACTTGGCCTT.................................................................................................................................................................................................................................................................................	23	2	0.50	1	0	1	0	0	0	0	0	0	0	0
.......................................................................................................................................................................................................................TAACTTTGCC---CCATTT-GG-AA-----------------------------A...........................................................................................................................................	21	2	0.50	1	0	1	0	0	0	0	0	0	0	0
.................GGGTT---------AGT-------TGGACAGTGGGTA...................................................................................................................................................................................................................................................................................................................................................................	21	2	0.50	1	0	1	0	0	0	0	0	0	0	0
.................................................................................................................CGCATGTACCGATACTTGGCC...................................................................................................................................................................................................................................................................................	21	2	0.50	1	0	1	0	0	0	0	0	0	0	0
..........................................................................................AG-CT--CGTACCTTCAAAGCGCC.......................................................................................................................................................................................................................................................................................................	21	2	0.50	1	0	1	0	0	0	0	0	0	0	0
...............GTGGGTT---------AGT-------TGGACAGTG.......................................................................................................................................................................................................................................................................................................................................................................	19	2	0.50	1	0	0	0	0	0	0	0	0	1	0
..................................................................................................................GCATGTACCGATACTTGGCCACC................................................................................................................................................................................................................................................................................	23	2	0.50	1	0	0	0	0	1	0	0	0	0	0
...............................................................G-CAAGCA---------GAGAT-AG-TAG-CT--CG......................................................................................................................................................................................................................................................................................................................	21	2	0.50	1	0	0	0	0	0	0	1	0	0	0
.......................................................................................................................TACCGATACTTGGCCACCGTG.............................................................................................................................................................................................................................................................................	21	2	0.50	1	0	1	0	0	0	0	0	0	0	0
........................................................................................................................................................TTGTTGTGCAGCTTCCGCA..............................................................................................................................................................................................................................................	19	2	0.50	1	0	1	0	0	0	0	0	0	0	0
................TGGGTT---------AGT-------TGGACAGTGG......................................................................................................................................................................................................................................................................................................................................................................	19	2	0.50	1	0	1	0	0	0	0	0	0	0	0
......................................................................................................................................................CATTGTTGTGCAGCTTCCG................................................................................................................................................................................................................................................	19	2	0.50	1	0	1	0	0	0	0	0	0	0	0
.........................................................................................................CAAAGCGCCGCATGTACCGATA..........................................................................................................................................................................................................................................................................................	22	2	0.50	1	0	1	0	0	0	0	0	0	0	0
................................................................................................................................TTGGCCACCGTGTGAGATATG....................................................................................................................................................................................................................................................................	21	2	0.50	1	0	0	0	0	0	0	1	0	0	0
.............................................................................................CT--CGTACCTTCAAAGCGCCGC.....................................................................................................................................................................................................................................................................................................	21	2	0.50	1	0	0	0	0	0	0	1	0	0	0
................................GT-------TGGACAGTGGGTACGC................................................................................................................................................................................................................................................................................................................................................................	18	2	0.50	1	0	1	0	0	0	0	0	0	0	0
............................................................................................................................ATACTTGGCCACCGTGTGAG.........................................................................................................................................................................................................................................................................	20	2	0.50	1	0	0	0	0	0	0	1	0	0	0
.................GGGTT---------AGT-------TGGACAGTGGG.....................................................................................................................................................................................................................................................................................................................................................................	19	2	0.50	1	0	0	0	0	0	0	0	0	1	0
...........................................................................................................................................GTGAGATATGCCATTGTTGTG.........................................................................................................................................................................................................................................................	21	2	0.50	1	0	1	0	0	0	0	0	0	0	0
....................................................................................................................................................................................................................AAATAACTTTGCC---CCATTT-G.............................................................................................................................................................................	20	2	0.50	1	0	1	0	0	0	0	0	0	0	0
..............................................................................................................................................................................................................TCACAAAAATAACTTTGCC---C....................................................................................................................................................................................	20	2	0.50	1	0	1	0	0	0	0	0	0	0	0
................................GT-------TGGACAGTGGGTACGCTCT.............................................................................................................................................................................................................................................................................................................................................................	21	2	0.50	1	0	1	0	0	0	0	0	0	0	0
.............................................................TTG-CAAGCA---------GAGAT-AG-TAG-............................................................................................................................................................................................................................................................................................................................	19	2	0.50	1	0	0	0	0	0	0	0	0	1	0
.................................................................................AGAT-AG-TAG-CT--CGTACCTTCA..............................................................................................................................................................................................................................................................................................................	21	2	0.50	1	0	0	0	0	0	0	1	0	0	0
........................................................................................................TCAAAGCGCCGCATGTACCGA............................................................................................................................................................................................................................................................................................	21	2	0.50	1	0	1	0	0	0	0	0	0	0	0
.............................................................................................................................TACTTGGCCACCGTGTGAGAT.......................................................................................................................................................................................................................................................................	21	2	0.50	1	0	1	0	0	0	0	0	0	0	0
...............GTGGGTT---------AGT-------TGGACAGTGGCC....................................................................................................................................................................................................................................................................................................................................................................	22	2	0.50	1	0	0	0	0	0	0	0	0	1	0
...................GTT---------AGT-------TGGACAGTGGGTACT.................................................................................................................................................................................................................................................................................................................................................................	21	2	0.50	1	0	0	0	0	0	0	0	0	1	0
....................................................................................................................................CCACCGTGTGAGATATGCCAT................................................................................................................................................................................................................................................................	21	2	0.50	1	0	1	0	0	0	0	0	0	0	0
..............AGTGGGTT---------AGT-------TGGACAGTGGGT....................................................................................................................................................................................................................................................................................................................................................................	23	2	0.50	1	0	1	0	0	0	0	0	0	0	0
.......................................................................................................................................................................................................................................TTT-GG-AA-----------------------------AGGGGTGAAAATG...............................................................................................................................	20	2	0.50	1	0	1	0	0	0	0	0	0	0	0
....................................................................................................................................................................................................................AAATAACTTTGCC---CCATTT-GG-...........................................................................................................................................................................	21	2	0.50	1	0	1	0	0	0	0	0	0	0	0
...................................................................................................................................................TGCCATTGTTGTGCAGCTTC..................................................................................................................................................................................................................................................	20	2	0.50	1	0	1	0	0	0	0	0	0	0	0
.....................T---------AGT-------TGGACAGTGGGTAA..................................................................................................................................................................................................................................................................................................................................................................	18	2	0.50	1	0	0	0	0	0	0	0	0	1	0
...............................................................................................................GCCGCATGTACCGATACTTGG.....................................................................................................................................................................................................................................................................................	21	2	0.50	1	0	1	0	0	0	0	0	0	0	0
.................................................................................................................CGCATGTACCGATACTTGGC....................................................................................................................................................................................................................................................................................	20	2	0.50	1	0	1	0	0	0	0	0	0	0	0
..............AGTGGGTT---------AGT-------TGGACAGTGGGTACG.................................................................................................................................................................................................................................................................................................................................................................	26	2	0.50	1	0	0	0	0	0	0	0	0	1	0
....................TT---------AGT-------TGGACAGTGGGTACG.................................................................................................................................................................................................................................................................................................................................................................	20	2	0.50	1	0	0	0	0	0	0	0	0	1	0
...............................................GTGGGTACGCTCTGTTG-CAAG....................................................................................................................................................................................................................................................................................................................................................	21	2	0.50	1	0	1	0	0	0	0	0	0	0	0
..............................................................TG-CAAGCA---------GAGAT-AG-TAG-A...........................................................................................................................................................................................................................................................................................................................	19	2	0.50	1	0	0	0	0	0	0	1	0	0	0
....................................................................................................................................CCACCGTGTGAGATATGCCATC...............................................................................................................................................................................................................................................................	22	2	0.50	1	0	0	0	0	0	0	1	0	0	0
.................................T-------TGGACAGTGGGTACGCTC..............................................................................................................................................................................................................................................................................................................................................................	19	2	0.50	1	0	0	0	0	0	0	1	0	0	0
...............................................................G-CAAGCA---------GAGAT-AG-TAG-CT--C.......................................................................................................................................................................................................................................................................................................................	20	2	0.50	1	0	1	0	0	0	0	0	0	0	0
...................................................................................................................................................TGCCATTGTTGTGCAGCTTCC.................................................................................................................................................................................................................................................	21	2	0.50	1	0	1	0	0	0	0	0	0	0	0
....................................................................................T-AG-TAG-CT--CGTACCTTCAAA............................................................................................................................................................................................................................................................................................................	20	2	0.50	1	0	0	0	0	0	0	0	0	1	0
...................GTT---------AGT-------TGGACAGTGGGTA...................................................................................................................................................................................................................................................................................................................................................................	19	2	0.50	1	0	0	0	0	0	0	0	0	1	0
.......................................................................................................................................................ATTGTTGTGCAGCTTCCGC...............................................................................................................................................................................................................................................	19	2	0.50	1	0	1	0	0	0	0	0	0	0	0
..............................................AGTGGGTACGCTCTGTTG-CA......................................................................................................................................................................................................................................................................................................................................................	20	2	0.50	1	0	1	0	0	0	0	0	0	0	0
...AA----AC---AGTGGGTT---------AGT-------TGGAC...........................................................................................................................................................................................................................................................................................................................................................................	20	2	0.50	1	0	0	0	0	0	0	0	0	1	0
....................................................................................................................................................................................CACTTTTCATAAAACAACCGC................................................................................................................................................................................................................	21	2	0.50	1	0	1	0	0	0	0	0	0	0	0
............................................................................................................................................................................................................................TTGCC---CCATTT-GG-AA-----------------------------AGGG........................................................................................................................................	19	2	0.50	1	0	0	0	0	0	0	1	0	0	0
..............................................AGTGGGTACGCTCTGTTG-........................................................................................................................................................................................................................................................................................................................................................	18	2	0.50	1	0	1	0	0	0	0	0	0	0	0
...............................................................................................................................CTTGGCCACCGTGTGAGATATA....................................................................................................................................................................................................................................................................	22	2	0.50	1	0	0	0	0	0	0	0	0	1	0
....................................................................................................................................................GCCATTGTTGTGCAGCTTCCG................................................................................................................................................................................................................................................	21	2	0.50	1	0	1	0	0	0	0	0	0	0	0
.............................................................................................................................................................................................................................TGCC---CCATTT-GG-AA-----------------------------AGGGGTG.....................................................................................................................................	21	2	0.50	1	0	1	0	0	0	0	0	0	0	0
...................................................................................................TACCTTCAAAGCGCCGCATG..................................................................................................................................................................................................................................................................................................	20	2	0.50	1	0	1	0	0	0	0	0	0	0	0
...............................AGT-------TGGACAGTGGGTACGCT...............................................................................................................................................................................................................................................................................................................................................................	20	2	0.50	1	0	1	0	0	0	0	0	0	0	0
..........................................................................................................................................TGTGAGATATGCCATTGTTG...........................................................................................................................................................................................................................................................	20	2	0.50	1	0	1	0	0	0	0	0	0	0	0
...................................................................................................TACCTTCAAAGCGCCGCATGT.................................................................................................................................................................................................................................................................................................	21	2	0.50	1	0	1	0	0	0	0	0	0	0	0
..........................................................................................................AAAGCGCCGCATGTACCGATA..........................................................................................................................................................................................................................................................................................	21	2	0.50	1	0	0	0	0	0	0	1	0	0	0
....................................................................................................................ATGTACCGATACTTGGCCA..................................................................................................................................................................................................................................................................................	19	2	0.50	1	0	1	0	0	0	0	0	0	0	0
................................................TGGGTACGCTCTGTTG-CAAG....................................................................................................................................................................................................................................................................................................................................................	20	2	0.50	1	0	0	0	0	0	0	0	0	1	0
...................................................................................................................................................................................................................AAAATAACTTTGCC---CCATTT-G.............................................................................................................................................................................	21	2	0.50	1	0	1	0	0	0	0	0	0	0	0

droPer2

GTCAA----AC---AGTGGGTT---------AGT-------TGGACAGTGGGTACGCTCTGTTG-CAAGCA---------GAGAT-AG-TAG-CT--CGTACCTTCAAAGCGCCGCATGTACCGATACTTGGCCACCGTGTGAGATATGCCATTGTTGTGCAGCTTCCGCACATTCAAGTCACTTTTCATAAAACAACCGCAGTAATCACAAAAATAACTTTGCC---CCATTT-GG-AA-----------------------------AGGGGTGAAAATGTAAA----------CAAT-------------------------AACTTTTCGTCCTAGAGATGGTTAA-----------------------------------------------------------	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
..............................................................................................................................................................TGCAGCTTCCGCACATTCAA.......................................................................................................................................................................................................................................	20	3	0.33	1	0	0	1	0	0	0

droWil2

GTTTA----CA---ATATATGT-----ACATATG------TAT-T-------------TTAT--------------TTGT-----GGGAAGT-CC--CTTACTTTCGAATCTTTGCATGTAGGTCACCTTGGCTGCTGTATGCAGCATGCCAGCGTTGTGCAATTTACGCACATTAATATCATTTTTCATAAAACAACAACAATAATCGCAATAATAACTTTTGCCACCCATTT-TTTTC-----------------------------AGAAATTA-------------------------------------------------------------AAAAATAACAAATATAA------------------------------------------------------	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
.................................................................................................................TGCATGTAGGTCACCTTGGC....................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	1	0	0	0

droVir3

ATACGTTAATA----TT--ATT---------TA------TTAT-----------------GCA--------TTTAAATGC-----AAGCGCT-AG--CTCACCCTCAAAGCGACGCAGGTAGGTTGCCTTCGCAGCGGTGTGCGAAGCACCCGCATTGTGCAGCTTGCGCACATTTAGGTCATTTTTCATATAGCAACAGCAGTAATCACAATAATAGCTTTTGCCGCCCATTT-AC----------------------------------AA--------ATAAA----------AAAAA-A----------------A-----------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M027 Male-body	M028 Head	M047 Female-body	M061 Embryo	SRR060654 Ovary	SRR060655 Testis	SRR060656 Ovary	SRR060657 Testis	SRR060658 Ovary	SRR060659 Testis	SRR060660 Ovary	SRR060661 Embryo	SRR060662 Embryo	SRR060663 Embryo	SRR060664 Male-body	SRR060665 Female-body	SRR060666 Male-body	SRR060667 Female-body	SRR060668 Male-body	SRR060669 Female-body	SRR060670 Testis	SRR060671 Male-body	SRR060672 Female-body	SRR060673 Ovary	SRR060674 Ovary	SRR060675 Ovary	SRR060676 Ovary	SRR060677 Ovary	SRR060678 Testis	SRR060679 Testis	SRR060680 Testis	SRR060681 Testis	SRR060682 Embryo	SRR060683 Testis	SRR060684 Embryo	SRR060685 Embryo	SRR060686 Embryo	SRR060687 Embryo	SRR060688 Ovary	SRR060689 Testis	SRR1106712 embryo_0-2h	SRR1106713 embryo_0-2h	SRR1106714 embryo_2-4h	SRR1106715 embryo_4-6h	SRR1106716 embryo_4-6h	SRR1106717 embryo_6-8h	SRR1106718 embryo_6-8h	SRR1106719 embryo_8-10h	SRR1106720 embryo_8-10h	SRR1106721 embryo_10-12h	SRR1106722 embryo_10-12h	SRR1106723 embryo_12-14h	SRR1106724 embryo_12-14h	SRR1106725 embryo_14-16h	SRR1106726 embryo_14-16h	SRR1106727 larvae	SRR1106728 larvae	SRR1106729 mixed whole adult body	SRR1106730 embryo_16-30h	V047 Embryo	V053 Head	V116 Male-body	GSM1528803 follicle cells
.......................................................................................................................................................................................TTTTCATATAGCAACAGCAG..............................................................................................................................................................................................................	20	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..................................................................................................TCACCCTCAAAGCGACGCAGG..................................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.....................................................................................................................................................CCCGCATTGTGCAGCTTGCG................................................................................................................................................................................................................................................	20	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..................................................................................................TCACCCTCAAAGCGACGC.....................................................................................................................................................................................................................................................................................................	18	1	1.00	1	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................................AT-----------------GCA--------TTTAAATGC-----AAGCGCT-AG--........................................................................................................................................................................................................................................................................................................................	23	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..................................................................................................................................CGCAGCGGTGTGCGAAGCA....................................................................................................................................................................................................................................................................	19	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

droMoj3

AAAAA----TAGAATTTAAT-AA--------AT--------AT------------TTTTTGCT---------------TA-----ACTAAATATCCCAGTACCTTCAAATCGACGCATATAGGTTGCCTTGGCAGCCGTGTGTGAAATGCCTGCATTGTGTAATTTACGCACGTTTAGATCATTTTTCATAAAGCAACAACAGTAATCACAATAATAGCTTTTTCCACCCATTT-AG-TA-----------------------------AATT--------ACAAA---------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M046 Female-body	V041 Embryo	V049 Head	V056 Head	M060 Embryo	V110 Male-body
................................................................................................CAGTACCTTCAAATCGAC.......................................................................................................................................................................................................................................................................................................	18	1	1.00	1	0	0	0	0	1	0

droGri2

ATTAT----AA---ATTATTTT---------T---------------------------AGCTTAA-----AGATAAACAA----AACTAA--AG--CCCACCCTCAAATTGCCGCATGTAAGTTGCTTTTGCAGCTGTGTGCGAAATGCCCGAATTGTGTAATTTGCGCACATTCAAATCGTTCTTCATGAAGCAACAGCAGTAATCACAGTAATAACTTTTGCCACCCATTA-TT----------------------------------AA--------CTAAA----------C----------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M041 Female-body	V109 Male-body
No Reads

dsi_9431

2l:1117707-1117856 -

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-7.2	-7.0	-7.0