dsi_6021

five_prime_UTR [3r_9855171_9855233_+]; exon [3r_9855171_9855273_+]; five_prime_UTR [3r_9854895_9855101_+]; exon [3r_9854895_9855101_+]; intron [3r_9855102_9855170_+]

##################################################---------------------------------------------------------------------################################################## TGATCAGTCAGTCAGTCGAACAGCGACACTCGCAGCGACCGGAGCAAAAAGTGAGCAGCAAGTGGAAAATAATGGAAAGCCGCCTCTGGCCAAGTCGCAACTAATTGAATTATTTATAGAAAACACTTCAGCAGCTGACATTTAAGTTTTTGCTACCCCGTTTTTGCTG ************************************************((((...((.((.((.......(((....))))).)).))....)))).....................************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M024 male body	M023 head	M053 female body	SRR618934 dsim w501 ovaries	SRR553486 Makindu_3 day-old ovaries	O002 Head	SRR902009 testis	O001 Testis	SRR553485 Chicharo_3 day-old ovaries	GSM343915 embryo	SRR553488 RT_0-2 hours eggs	M025 embryo
.......................................................................................................................................................GCTACCCCGTTTTTGCTG	18	0	1	3.00	3	3	0	0	0	0	0	0	0	0	0	0	0
.......................CGACACTCGCAGCGACCGGAG.............................................................................................................................	21	0	1	2.00	2	0	0	0	0	0	0	2	0	0	0	0	0
..........................CACTCGCAGCGACCGGAGC............................................................................................................................	19	0	1	2.00	2	1	0	1	0	0	0	0	0	0	0	0	0
...................................CGACCGGAGCAAAAAGTG....................................................................................................................	18	0	1	2.00	2	0	2	0	0	0	0	0	0	0	0	0	0
..................................................................AAATAATGGAAAGCCGCCTCTGG................................................................................	23	0	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0
....................................................................................TCTGGCCAAGTCGCAACTAATTGAAT...........................................................	26	0	1	1.00	1	0	0	0	0	0	0	0	0	1	0	0	0
...TCAGTCAGTCAGTCGAACAGCGACACT...........................................................................................................................................	27	0	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
...............................................................GGAAAATAATGGAAAGCCGCCTCTG.................................................................................	25	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0
...........TCAGTCGAACAGCGACACTCG.........................................................................................................................................	21	0	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0
......................................................................AATGGAAAGCCGCCTCTG.................................................................................	18	0	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0
.................................................................................................................TTATAGAAAACACTTCAGCAGCTG................................	24	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0
.............AGTCGAACAGCGACACTCGCAGCG....................................................................................................................................	24	0	1	1.00	1	0	0	0	0	0	0	0	1	0	0	0	0
......................................................GCAGCAAGTGGAAAATAATGGAAAGC.........................................................................................	26	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0
...................................................................................................................ATAGAAAACACTTCAGCAGCTG................................	22	0	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0
................................................................GAAAATAATGGAAAGCCGCCTCTGG................................................................................	25	0	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0
...................................................................................................................AAAAAAAACACTTCAGCAGCTGAC..............................	24	2	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0
...................ACAGCGACACTCGCAGCGACCGGA..............................................................................................................................	24	0	1	1.00	1	0	0	0	0	0	1	0	0	0	0	0	0
.......................CGACACTCGCAGCGACCGGA..............................................................................................................................	20	0	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0
...........................ACTCGCAGCGACCGGAGC............................................................................................................................	18	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0
..........................................................................................................................ACACTTCAGCAGCTGACATTTAAGTT.....................	26	0	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0
TGATCAGTCAGTCAGTCGAACAGCGACACT...........................................................................................................................................	30	0	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0
.....................................................AGCAGCAAGTGGAAAATAATG...............................................................................................	21	0	1	1.00	1	0	0	0	0	0	1	0	0	0	0	0	0
........................GACACTCGCAGCGACCGGAGC............................................................................................................................	21	0	1	1.00	1	0	0	0	0	0	0	0	0	0	1	0	0
...................................................................................................TCTAATCGATTTATTTATAGAAA...............................................	23	3	3	0.33	1	0	0	0	0	0	0	0	0	0	0	1	0
.......................................................................................................ATTGAATTATTTAGTGAAAA..............................................	20	2	4	0.25	1	0	0	0	0	1	0	0	0	0	0	0	0
.................................................................................................................................AGCAGCTGACACTTAA........................	16	1	6	0.17	1	0	0	0	0	0	0	0	0	0	0	1	0
.......................CGACACTCGTAGCGAAAGGA..............................................................................................................................	20	3	6	0.17	1	0	0	0	0	1	0	0	0	0	0	0	0
......................GCAACACTCGCAGCGA...................................................................................................................................	16	1	9	0.11	1	0	0	0	1	0	0	0	0	0	0	0	0
.................................................................................................................TTATAGAACACATTTCTGCA....................................	20	3	15	0.07	1	0	0	0	0	0	0	0	0	0	0	0	1

ACTAGTCAGTCAGTCAGCTTGTCGCTGTGAGCGTCGCTGGCCTCGTTTTTCACTCGTCGTTCACCTTTTATTACCTTTCGGCGGAGACCGGTTCAGCGTTGATTAACTTAATAAATATCTTTTGTGAAGTCGTCGACTGTAAATTCAAAAACGATGGGGCAAAAACGAC ************************************************((((...((.((.((.......(((....))))).)).))....)))).....................************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	SRR553485 Chicharo_3 day-old ovaries	SRR553487 NRT_0-2 hours eggs	M025 embryo	SRR618934 dsim w501 ovaries	SRR553488 RT_0-2 hours eggs
..............................................................................AGGCGGAGACCGGTTCAGCGTTGATT.................................................................	26	1	1	2.00	2	2	0	0	0	0
.....................................................................................GACCGGTTCAGCGTTGATTAACTT............................................................	24	0	1	1.00	1	0	1	0	0	0
.........................................................................................................................TTGTTAAGTCGTCGACTGTAAATTCAA.....................	27	1	1	1.00	1	1	0	0	0	0
............................................................................................CCAGCTTTGATTGACTTAAT.........................................................	20	3	18	0.11	2	0	0	2	0	0
..............................................................................................................................AAGTCGTCGCCTGTATA..........................	17	2	10	0.10	1	0	0	0	1	0
..........................................................................................................................................GTAACTTCATAAACGATG.............	18	2	11	0.09	1	0	1	0	0	0
...............................TGTCGGTGGCCTCGTTT.........................................................................................................................	17	2	20	0.05	1	0	0	0	0	1

Species	Coordinate	ID	Alignment
droSim2	3r:9855052-9855220 +	dsi_6021	TGATCAGTCAGTCAGTCGAACAGCGACACTCGCAGCGACCGGAGC-A----------------AAAAGTGAGCAGCAA----------GTGGA-AAATAATGGAAAGCCGCCTCTGGCCAAGTCG---------------------CAACTAATTGAAT---------------------TATTTATAGAAA--ACACT-T-CAGCAGC------TGACATTTAAGTT-TTTGC-TACCCCGTTT-------------------------TTGCTG-
droSec2	scaffold_0:10562935-10563103 +		TGATAATTCAGTCAGTCGAACAGCGACACTCGCAGCGACCGGAGC-A----------------AAAAGTGAGCAGCAA----------GTGGA-AAATAATGGAAAGCCGCCTCTGGCCAAGTCG---------------------CAACTAATTTAAT---------------------TACTTGTAGAAA--ACACT-T-CAGCAGC------TGACATTTAAGTT-TTTGC-TACCCCGTTT-------------------------TTGCTG-
dm3	chr3R:11373130-11373292 -		TGATC----AGTCAGTCGAACAGCAACACTCGCAGCGACCGGAGC-A----------------AAAAGTGAGCAGCAA----------ATGGA-AAATAATGGAAAGCC---ACTGGCCAAGTCG---------------------CAACTAATTGAAT---------------------TATTTATAGAAA--ACACT-T-CAGCAGC------TGACATTTTAGTT-TTTGC-TACGCCGTTT------------------------TTTGCTG-
droEre2	scaffold_4770:10901321-10901473 -		TG--------ACCAGTCGAACAGCGACACTCGCAGCCAGCGGAGC-A----------------ACCAGTGAGC---AA----------GTGGA-AAATAATGGAAAGCC---TCTGGC-AAATCA---------------------CAGCTAATTGAAT---------------------TATTTATAGAAA--ACACT-T-GAGCAGC------TGACATTTAAGTT-CTGGT-TACCCCGTT--------------------------TTGCTG-
droYak3	3R:15053283-15053428 +		TG--------ATCAGTCGAACAGCGACACTCGCAGCAAGCGGACT-A----------------AAAAGTGAGCAGCAA----------GTGGA-AAATAATGGAAATTC---TC---------------------------------AGCTAATTGAAT---------------------TATTTATAGAAA--ATACT-T-CGGCAGC------TGACATTTAAGTG-TTTGC-TACCCCGTTT-------------------------TTGCTG-
droEug1	scf7180000409797:2218252-2218421 +		TTAG----CGATCAGTTGAGCAACGACACTCGCAGCGACCGGAGC-T-------AAGCACAAGAAAAGTGAGCAGCAA----------AGGAA-AAATAATGGAAAACC---TCT-CGAAAGTCA---------------------CAACTAATTGAAT---------------------TATTTATAGAAA--ACGCT-T-CAGCAGC------TGACATTTAAGTT-TTTGT-TGCCCTGTTA-------------------------TTGTTG-
droBia1	scf7180000302136:976333-976499 -		CG--------ATCAGTTGAGCAACGACACTCGCAGCGACCGGAGC-C----------------AAAAGTGAGCAGCAA----------ATGGA-AAATAGTGGAAACCT---TCTGCCAAAGTCACA-------------------AAACTAATTGAAT---------------------TATTTATAGCAA--ACACT-T-CAGCAGCTTGAATTGACATTTGAATT-GTTGT-TGCCCTGTTA-------------------------TTGTTGA
droTak1	scf7180000415769:1785996-1786149 +		CG--------ATCAGTTGAGCAACGGCAGTCGCAGCGACCGGAGTGA----------------AAAAGTGAGCAGCAA----------ATGGA-AAATAATGGAAAACC---TCGACCAAAGGCACT-------------------AAACTAATTGAAT---------------------TATTTATAGAAA--ACACT-A-CAGCAGC------TGACATTTGAGTT-TTTGT-TGCCCTGTT---------------------------------
droEle1	scf7180000491080:1150408-1150568 -		CG--------ATCAGTTGAGCAACGGCACTCGAAGCGACCGGAGC-C----------------AAAAGTAAGCAGCAA----------ACGGA-AAATAATGGAAAACC---CCAGCCAGTGTCA---------------------CAACTAATTGAAT---------------------TATTTATAGAAAAAACACT-T-CAGCAGC------TGACATTTGAGTT-TTTGT-TGCCCTGTTA-------------------------TTGTTGA
droRho1	scf7180000779997:83242-83400 +		TG--------ATCAGTTGAGCAACGGCACTCGGAGCGACCGGAGC-C----------------AAAAGTAAGCAACCA----------ATGGA-AAATAATGGAAAACC---TCAGCCAGTGTCA---------------------AAACTAATTGAAT---------------------TATTTATAGAAA--ACATT-T-GAAAAGC------TGACATTTCAGTC-TTTGT-TGCCTTGTTG-------------------------TTGTTGA
droFic1	scf7180000452523:735864-736022 -		CG--------ATCAGTTGAGCAACGACACTCGAAGCGACCGGAG--A----------------AAAAGTGAGCAGCCA----------GCGGA-AAATAGTGGAAAACC---TCTGCCAAAGTCA---------------------CAACTAATTGAAT---------------------TATTTATAGAAA--ACACT-TCAAGCAGC------TGACATTTAAGTC-ATTGT-TGTCCCGTTT------------------------CTTGTTG-
droKik1	scf7180000302639:1298532-1298708 +		TGATTTCGCGATCAGTTGAACAACAACACCCGCGGCGAACGGAGG-C----------------ACCAGTGAGCAGCAA----------GTGGA-AAATAATGGAAAACC---TAGGCAAAAGTCA----------------------AACTAATTGAATT----------TTTTTTTCTGTATTTATAGAAA--ACTCT-T-TCACAGC------TGACATTTAGGTA-TTTGTTTGCTTCGTTG-------------------------TTGTTG-
droAna3	scaffold_13340:15996649-15996838 -		TGATTACTCGATCAGTTGGCCTGAGAAGCTCGGAGCAAACGGAGG-C----------------AATAGCTCCCAGCAG----------GCTAT-ATATAATAGAAAACA---GAAGCAACCGTCT-T-------------------ACACCAATTGAAT---------------------TATTTATAGAAT--ATAAT-T-CAGCATC------TGACATTTAAGTTATTTGT-TGCCTTGTTAGAGCCGA---GAACAACAATAAGGTTTATTG-
droBip1	scf7180000396708:4036143-4036343 +		TGATTACTCGATCAGTTGGCCAGAGAAACTCGGAGCAACCGGAGG-C----------------AATAGCTCCCAGCAGGCT---ACATATGTACATATAATAGAAAACA---GAAGCAACCGTCT-T-------------------ACACTAATTGAAT---------------------TATTTATAGAGT--ATATT-T-CAGCATC------TGACATTTAAGTTATTTGT-TGCTTTGTTAGAGCCGAGGAGCACAACAACAAGGTTTATTG-
dp5	2:30376062-30376230 -		GGAGCAGATCGTCAGTTAAGCAACGGCAGTG------CTTGTGGC-AAGAAGCGAACCT----CGCAGTGGAA-------CACCAGAGACGGA-AAACATTGGAAAATA---TCAA-AAAAGTGG---------------------CCACCAATTGAAT---------------------TATTTATAGAAA--AAACTAT-CAGCAGC------TGACATTTGAGTG--------AATTTGTTT-------------------------GTGGTG-
droPer2	scaffold_6:5768886-5769054 -		GAATCGATCGTTCAGTTAAGCAACGGCAGTG------CTTGTGGC-AAGAAGCGAACCT----CGCAGTGGAA-------CACCAGAGACGGA-AAACATTGGAAAATA---TCAA-AAAAGTGG---------------------CCACCAATTGAAT---------------------TATTTATAGAAA--AAACTAT-CAGCAGC------TGACATTTGAGTG--------AATTTGTTT-------------------------GTGGTG-
droWil2	scf2_1100000004902:2539664-2539874 +		TCAGCG-GCGATCAGTTGTCCAAAGACAGCCG------ACGGAGA-C----------------AGAAGCAAAC---AA----------CGGGA-AACTACTAAAAAAAG---AGAGCAAAAAGCACCAAACGTATAGGAACAGCAACAACAAAT-GCATTAGTAAAGCACATTTTTTCATTATTTATAGAAACAACAAA-T-TTACAGC------TGACATTTGTGTG-TT-----GCTTTGCTGG---------GTATAGTATTTATTTTTGGAG-

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droSim2

TGATCAGTCAGTCAGTCGAACAGCGACACTCGCAGCGACCGGAGC-A----------------AAAAGTGAGCAGCAA----------GTGGA-AAATAATGGAAAGCCGCCTCTGGCCAAGTCG---------------------CAACTAATTGAAT---------------------TATTTATAGAAA--ACACT-T-CAGCAGC------TGACATTTAAGTT-TTTGC-TACCCCGTTT-------------------------TTGCTG-	Size	Hit Count	Total Norm	Total	M023 Head	M024 Male-body	M025 Embryo	M053 Female-body	O001 Testis	O002 Head	SRR553485 Ovary	SRR553486 Ovary	SRR553487 Ovary	SRR553488 Ovary	SRR618934 Ovary	V044 Embryo	SRR902008 Ovary	SRR902009 Testis	SRR1275487 Male larvae	SRR1275485 Male prepupae	SRR1275483 Male prepupae
........................................................................................................................................................................................................................................GC-TACCCCGTTT-------------------------TTGCTG-	18	1	3.00	3	0	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.......................CGACACTCGCAGCGACCGGAG.........................................................................................................................................................................................................................................	21	1	2.00	2	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0
..........................CACTCGCAGCGACCGGAGC-.......................................................................................................................................................................................................................................	19	1	2.00	2	0	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0
...................................CGACCGGAGC-A----------------AAAAGTG...............................................................................................................................................................................................................	18	1	2.00	2	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..............................................................................................AAATAATGGAAAGCCGCCTCTGG................................................................................................................................................................	23	1	1.00	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
................................................................................................................TCTGGCCAAGTCG---------------------CAACTAATTGAAT---------------------.................................................................................................	26	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0
...TCAGTCAGTCAGTCGAACAGCGACACT.......................................................................................................................................................................................................................................................	27	1	1.00	1	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0
..........................................................................................GGA-AAATAATGGAAAGCCGCCTCTG.................................................................................................................................................................	25	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...........TCAGTCGAACAGCGACACTCG.....................................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
..................................................................................................AATGGAAAGCCGCCTCTG.................................................................................................................................................................	18	1	1.00	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
.......................................................................................................................................................................................TTATAGAAA--ACACT-T-CAGCAGC------TG............................................................	24	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.............AGTCGAACAGCGACACTCGCAGCG................................................................................................................................................................................................................................................	24	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0
.......................................................................GCAGCAA----------GTGGA-AAATAATGGAAAGC.........................................................................................................................................................................	26	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................................................................................................................................................................................ATAGAAA--ACACT-T-CAGCAGC------TG............................................................	22	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...........................................................................................GA-AAATAATGGAAAGCCGCCTCTGG................................................................................................................................................................	25	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...................ACAGCGACACTCGCAGCGACCGGA..........................................................................................................................................................................................................................................	24	1	1.00	1	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0
.......................CGACACTCGCAGCGACCGGA..........................................................................................................................................................................................................................................	20	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0
...........................ACTCGCAGCGACCGGAGC-.......................................................................................................................................................................................................................................	18	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..................................................................................................................................................................................................ACACT-T-CAGCAGC------TGACATTTAAGTT-................................................	26	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0
TGATCAGTCAGTCAGTCGAACAGCGACACT.......................................................................................................................................................................................................................................................	30	1	1.00	1	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0
......................................................................AGCAGCAA----------GTGGA-AAATAATG...............................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0
........................GACACTCGCAGCGACCGGAGC-.......................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0

droSec2

TGATAATTCAGTCAGTCGAACAGCGACACTCGCAGCGACCGGAGC-A----------------AAAAGTGAGCAGCAA----------GTGGA-AAATAATGGAAAGCCGCCTCTGGCCAAGTCG---------------------CAACTAATTTAAT---------------------TACTTGTAGAAA--ACACT-T-CAGCAGC------TGACATTTAAGTT-TTTGC-TACCCCGTTT-------------------------TTGCTG-	Size	Hit Count	Total Norm	Total	M054 Female-body	V113 Male-body	V114 Embryo	V115 Head
..................................................................................................AATGGAAAGCCGCCTCTGG................................................................................................................................................................	19	1	4.00	4	0	0	0	4
..........................................................................GCAA----------GTGGA-AAATAATGG..............................................................................................................................................................................	18	1	2.00	2	0	0	1	1
...............................................................AAAAGTGAGCAGCAA----------GTG..........................................................................................................................................................................................	18	1	2.00	2	1	0	0	1
.......................................................................GCAGCAA----------GTGGA-AAATAATGGAAAGCCGC......................................................................................................................................................................	29	1	2.00	2	0	2	0	0
.........................................................................AGCAA----------GTGGA-AAATAATG...............................................................................................................................................................................	18	1	2.00	2	1	0	0	1
............................................................................................A-AAATAATGGAAAGCCGCCTCTGG................................................................................................................................................................	24	1	2.00	2	0	1	1	0
.................................................................................................TAATGGAAAGCCGCCTCTGG................................................................................................................................................................	20	1	2.00	2	0	0	0	2
...........................................................................................GA-AAATAATGGAAAGCCGCCTCTGG................................................................................................................................................................	25	1	2.00	2	0	0	0	2
...................................CGACCGGAGC-A----------------AAAAGTG...............................................................................................................................................................................................................	18	1	2.00	2	0	0	2	0
.......................................................................GCAGCAA----------GTGGA-AAATAATGGA.............................................................................................................................................................................	22	1	1.00	1	0	1	0	0
........................GACACTCGCAGCGACCGGAGC-A----------------......................................................................................................................................................................................................................	22	1	1.00	1	0	1	0	0
..............................................A----------------AAAAGTGAGCAGCAA----------GTG..........................................................................................................................................................................................	19	1	1.00	1	0	1	0	0
.....................AGCGACACTCGCAGCGACCGGAGC-.......................................................................................................................................................................................................................................	24	1	1.00	1	0	1	0	0
..................................................................................................AATGGAAAGCCGCCTCTGGCCAAGT..........................................................................................................................................................	25	1	1.00	1	0	1	0	0
...............................................................AAAAGTGAGCAGCAA----------GTGG.........................................................................................................................................................................................	19	1	1.00	1	0	0	1	0
........................GACACTCGCAGCGACCGGAGC-.......................................................................................................................................................................................................................................	21	1	1.00	1	0	0	1	0
.............................................................................A----------GTGGA-AAATAATGGAAAGCC........................................................................................................................................................................	21	1	1.00	1	0	1	0	0
.......................................................................GCAGCAA----------GTGGA-AAATAATGGAAA...........................................................................................................................................................................	24	1	1.00	1	0	0	0	1
...................................CGACCGGAGC-A----------------AAAAGTGAGCAGCAA----------G............................................................................................................................................................................................	27	1	1.00	1	0	0	1	0
.......................................................................GCAGCAA----------GTGGA-AAATAATGG..............................................................................................................................................................................	21	1	1.00	1	1	0	0	0
..........................................................................GCAA----------GTGGA-AAATAATGGAAAGCCG.......................................................................................................................................................................	25	1	1.00	1	0	1	0	0
.......................................................................................................................................................AATTTAAT---------------------TACTTGTAGA.......................................................................................	18	1	1.00	1	1	0	0	0
..........................CACTCGCAGCGACCGGAGC-.......................................................................................................................................................................................................................................	19	1	1.00	1	0	0	1	0
..........................................................................GCAA----------GTGGA-AAATAATGGAAAGCC........................................................................................................................................................................	24	1	1.00	1	0	0	1	0
..............................................................................................AAATAATGGAAAGCCGCCTCTGG................................................................................................................................................................	23	1	1.00	1	0	1	0	0
..................AACAGCGACACTCGCAGCGACCGGA..........................................................................................................................................................................................................................................	25	1	1.00	1	0	1	0	0
......................................CCGGAGC-A----------------AAAAGTGAGCAGCAA----------GTGG.........................................................................................................................................................................................	27	1	1.00	1	0	1	0	0
.......................................................................GCAGCAA----------GTGGA-AAATAATGGAAAGC.........................................................................................................................................................................	26	1	1.00	1	0	1	0	0
.......................................CGGAGC-A----------------AAAAGTGAGCAGCAA----------GTGGA-.......................................................................................................................................................................................	27	1	1.00	1	0	1	0	0
...............................................................AAAAGTGAGCAGCAA----------GTGGA-AAATA..................................................................................................................................................................................	25	1	1.00	1	1	0	0	0
.........................................................................AGCAA----------GTGGA-AAATAATGGAAAGC.........................................................................................................................................................................	24	1	1.00	1	0	1	0	0
..............................CGCAGCGACCGGAGC-A----------------AAAAGTG...............................................................................................................................................................................................................	23	1	1.00	1	0	1	0	0
..............................CGCAGCGACCGGAGC-A----------------AAAAGTGAGC............................................................................................................................................................................................................	26	1	1.00	1	0	1	0	0
...............................................................AAAAGTGAGCAGCAA----------GTGGA-A......................................................................................................................................................................................	21	1	1.00	1	0	0	0	1
.........................................................................................TGGA-AAATAATGGAAAGC.........................................................................................................................................................................	18	1	1.00	1	0	0	0	1
........................................................................................GTGGA-AAATAATGGAAAGCCG.......................................................................................................................................................................	21	1	1.00	1	0	1	0	0
..................AACAGCGACACTCGCAGCGACCGGAGC-.......................................................................................................................................................................................................................................	27	1	1.00	1	0	1	0	0
............................................................................................................CGCCTCTGGCCAAGTCG---------------------CAACT..............................................................................................................................	22	1	1.00	1	0	0	1	0
........................GACACTCGCAGCGACCGG...........................................................................................................................................................................................................................................	18	1	1.00	1	1	0	0	0
...................................CGACCGGAGC-A----------------AAAAGTGAGCAGCAA----------.............................................................................................................................................................................................	26	1	1.00	1	0	1	0	0
..............................................A----------------AAAAGTGAGCAGCAA----------GTGG.........................................................................................................................................................................................	20	1	1.00	1	0	1	0	0
..............................................A----------------AAAAGTGAGCAGCAA----------GTGGA-AA.....................................................................................................................................................................................	23	1	1.00	1	0	1	0	0
..........................................................................GCAA----------GTGGA-AAATAATGGAAAGCCGC......................................................................................................................................................................	26	1	1.00	1	1	0	0	0
....................................................................................................................................................................................................ACT-T-CAGCAGC------TGACATTTA.....................................................	20	1	1.00	1	1	0	0	0
...............................................................AAAAGTGAGCAGCAA----------GTGGA-AA.....................................................................................................................................................................................	22	1	1.00	1	0	1	0	0
............................................................................AA----------GTGGA-AAATAATGGAAAGCC........................................................................................................................................................................	22	1	1.00	1	1	0	0	0
..........................................................................GCAA----------GTGGA-AAATAATGGAAAGC.........................................................................................................................................................................	23	1	1.00	1	0	0	0	1
..................................................................................................AATGGAAAGCCGCCTCTGGCCAAGTCG---------------------C..................................................................................................................................	28	1	1.00	1	0	0	0	1
...................ACAGCGACACTCGCAGCGACCGGAGC-.......................................................................................................................................................................................................................................	26	1	1.00	1	0	0	1	0
.........................................................................AGCAA----------GTGGA-AAATAATGGAAAGCCG.......................................................................................................................................................................	26	1	1.00	1	1	0	0	0
...............................................................AAAAGTGAGCAGCAA----------GTGGA-AAA....................................................................................................................................................................................	23	1	1.00	1	0	1	0	0
.............................................................................................................GCCTCTGGCCAAGTCG---------------------CAACT..............................................................................................................................	21	1	1.00	1	1	0	0	0

dm3

TGATC----AGTCAGTCGAACAGCAACACTCGCAGCGACCGGAGC-A----------------AAAAGTGAGCAGCAA----------ATGGA-AAATAATGGAAAGCC---ACTGGCCAAGTCG---------------------CAACTAATTGAAT---------------------TATTTATAGAAA--ACACT-T-CAGCAGC------TGACATTTTAGTT-TTTGC-TACGCCGTTT------------------------TTTGCTG-	Size	Hit Count	Total Norm	Total	GSM154618 Ovary	GSM154620 Ovary	GSM154621 Ovary	GSM154622 Ovary	GSM180328 Head	GSM180329 Mixed-body	GSM180330 Embryo	GSM180331 Embryo	GSM180332 Embryo	GSM180333 Embryo	GSM180334 Larvae	GSM180335 Disc	GSM180336 Pupae	GSM180337 S2	GSM231091 Ovary	GSM239041 Head	GSM239050 Head	GSM239051 S2	GSM239052 S2	GSM246084 Head	GSM266765 S2	GSM280082 Ovary	GSM280083 Ovary	GSM280084 Ovary	GSM280085 Testis	GSM280086 Ovary	GSM280087 S2-NP	GSM280088 S2-NP	GSM312995 Head	GSM313160 Head	GSM313161 Head	GSM313162 Head	GSM313163 Head	GSM313164 Head	GSM313165 Head	GSM343287 Embryo	GSM379050 Ovary	GSM379051 Ovary	GSM379052 Ovary	GSM379053 Ovary	GSM379054 Ovary	GSM379055 Ovary	GSM379056 Ovary	GSM379057 Ovary	GSM379058 Ovary	GSM379059 Ovary	GSM379060 Ovary	GSM379061 Ovary	GSM379062 Ovary	GSM379063 Ovary	GSM379064 Ovary	GSM379065 Ovary	GSM379066 Ovary	GSM379067 Ovary	GSM424739 S2	GSM424740 S2	GSM424741 S2	GSM467729 Female-body	GSM467730 Female-body	GSM467731 Female-body	M002 CS	M003 Male-body	M004 Male-body	M005 ML-DmD32	M006 ML-DmD32	M029 OSC	M030 OSC	M031 OSC	M032 S2-R+	M033 S2-R+	M034 S2-R+	M035 S2-R+	M036 S2-R+	M037 S2-R+	M038 S2-R+	M039 S2-R+	M048 S2-R+	OSS2 OSS	OSS6 OSS	OSS7 OSS	OSS8 OSS	S1 Head	S10 Embryo	S11 Embryo	S12 Embryo	S13 Embryo	S14 Embryo	S15 S2-GMR	S16 Kc	S17 Larvae	S18 Larvae	S19 Pupae	S2 Head	S20 Pupae	S21 Disc	S22 Head	S23 Female-body	S24 Male-body	S25 Larvae	S26 Larvae	S27 Pupae	S28 Pupae	S29 Head	S3 Male-body	S30 S2-GMR	S31 S2	S32 S2	S33 Kc	S34 Kc	S35 Kc	S4 Female-body	S5 Disc	S6 Embryo	S7 Embryo	S8 S2	S9 Embryo	SRR001337 Head	SRR001338 Head	SRR001339 Head	SRR001340 Head	SRR001341 Head	SRR001342 Head	SRR001343 Head	SRR001344 Head	SRR001345 Head	SRR001346 Head	SRR001347 Head	SRR001348 Head	SRR001349 Head	SRR001664 Head	SRR010951 Ovary	SRR010952 Ovary	SRR010953 Ovary	SRR010954 Ovary	SRR010955 Ovary	SRR010956 Ovary	SRR010957 Ovary	SRR010958 Ovary	SRR010959 Ovary	SRR010960 Ovary	SRR014268 Embryo	SRR014269 Embryo	SRR014270 Embryo	SRR014271 Embryo	SRR014272 Embryo	SRR014273 Ovary	SRR014274 Ovary	SRR014275 Ovary	SRR014276 Ovary	SRR014277 Ovary	SRR014278 Ovary	SRR014279 Ovary	SRR014280 Ovary	SRR014281 Ovary	SRR014282 Ovary	SRR023197 S2	SRR023399 S2	SRR023400 S2	SRR023402 S2	SRR023407 S2	SRR029028 S2	SRR029029 S2	SRR029030 S2	SRR029031 S2	SRR029032 S2	SRR029033 S2	SRR029608 Head	SRR029633 Head	SRR030145 gb3	SRR031692 Head	SRR031693 Head	SRR031694 Head	SRR031696 Head	SRR031697 Head	SRR031698 Head	SRR031699 Head	SRR031700 Head	SRR031701 Head	SRR031702 Head	SRR031703 Head	SRR031704 Head	SRR032092 S2	SRR032093 S2	SRR032094 S2	SRR032095 S2-NP	SRR032096 S2-NP	SRR032152 Head	SRR060643 Testis	SRR060644 Ovary	SRR060645 Testis	SRR060646 Ovary	SRR060647 Ovary	SRR060648 Ovary	SRR060649 Ovary	SRR060650 Testis	SRR060651 Ovary	SRR060652 Testis	SRR060653 Ovary	SRR065151 Ovary	SRR065152 Ovary	SRR065153 Ovary	SRR065154 Ovary	SRR065155 Ovary	SRR065800 Ovary	SRR065801 Ovary	SRR065802 Ovary	SRR065803 Ovary	SRR065804 Ovary	SRR065805 Ovary	SRR065806 Ovary	SRR065807 Ovary	SRR097865 S2-NP	SRR097866 S2-NP	SRR097867 S2-NP	SRR298536 Ovary	SRR298537 Ovary	SRR298711 Ovary	SRR298712 Ovary	SRR317110 Ovary	SRR317111 Ovary	SRR317112 Ovary	SRR317113 Ovary	SRR317114 Ovary	SRR317115 Ovary	SRR317116 Ovary	SRR317117 Ovary	SRR341115 S2	SRR341116 S2	SRR341117 S2	SRR341118 S2	SRR351332 Mixed-body	SRR351333 Mixed-body	T004 OSC	T005 CME-L1	V001 Embryo	V002 Embryo	V003 S2	V006 Female-body	V007 Head	V008 S2	V009 CME-L1	V010 ML-DmD20-C5	V011 Sg4	V012 Male-body	V013 Head	V014 DTT	V015 DreRFHV	V018 Kc167	V019 GM2	V020 S2-R+	V021 ML-DmD21	V022 ML-DmD32	V023 Head	V024 Kc167	V025 S2-R+	V026 1182-2H	V027 ML-DmD21	V028 CME-W1-Cl.8+	V029 ML-DmD9	V030 ML-DmD8	V031 GM2	V032 S1	V033 CME-W1-Cl.8+	V034 ML-DmD16-C3	V035 ML-DmD32	V036 ML-DmD20-C5	V037 S2	V038 S2	V063 Ovary	V0632 Ovary	V064 Ovary	V0642 Ovary	V065 Ovary	V0652 Ovary	V066 Ovary	V0662 Ovary	V067 S2-NP	V073 mbn2	V074 S3	V075 ML-DmBG1-C1	V076 ML-DmBG3-C2	V077 Head	V078 Head	V079 Head	V080 Head	V081 Embryo	V082 Embryo	V083 Male-body	V084 Female-body	V085 CME-W2	V086 Female-body	V088 Ovary	V0882 Ovary	V089 Ovary	V0892 Ovary	V090 Male-body	V091 fGS/OSS	V092 Male-body	V093 Male-body	V094 S2-R+	V095 S2-R+	V096 Ovary	V097 Ovary	V098 Ovary	V125 ML-DmD9	V126 CME-L1	V127 G2	V128 S3	V129 ML-DmBG1-C1	V130 ML-DmBG3-C2	V131 ML-DmD16-C3	V132 ML-DmD32	V133 Sg4	V134 ML-DmD8	V135 CME-W2	V136 Male-body	V137 Head	V138 Male-body	V139 Female-body	V140 Female-body	V141 Female-body	V142 Female-body	V144 OSC	V145 S2	V146 S1	V147 1182-2H	V148 mbn2	GSM1528798 follicle cells
...................................CGACCGGAGC-A----------------AAAAGTG...............................................................................................................................................................................................................	18	1	164.00	164	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	9	6	5	4	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	2	0	0	0	0	0	0	1	1	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	6	1	0	0	0	0	0	1	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	1	0	0	5	7	2	0	0	0	0	0	6	3	5	2	6	0	5	4	1	3	0	0	0	2	6	0	4	2	0	1	0	0	0	0	0	0	0	0	0	0	2	3	0	0	0	1	0	0	0	0	1	0	4	0	1	5	0	1	0	0	0	1	0	0	1	1	0	0	3	1	4	0	0	2	0	0	3	0	0	0	0	0	0	0	0	0	6	10	1	0	0
.........................ACACTCGCAGCGACCGGA..........................................................................................................................................................................................................................................	18	1	35.00	35	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	8	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	1	0	0	0	0	0	1	0	0	0	0	0	0	0	0	1	0	0	0	0	1	0	0	1	2	0	0	0	0	0	0	0	0	0	0	1	1	2	0	0	0	0	0	0	0	0	0	0	2	0	1	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	0	0	0	1	2	0	0	0
...............................................................AAAAGTGAGCAGCAA----------ATG..........................................................................................................................................................................................	18	1	25.00	25	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	1	0	0	0	0	0	8	0	0	1	0	0	0	1	0	0	4	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	3	0	0	0
.....................................ACCGGAGC-A----------------AAAAGTGAG.............................................................................................................................................................................................................	18	1	23.00	23	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	1	1	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	1	0	0	0	1	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	1	1	0	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	4	1	0	0	1	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	1	0	0	1	0	0	0
.............AGTCGAACAGCAACACTCG.....................................................................................................................................................................................................................................................	19	1	19.00	19	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	2	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	1	0	0	0	0	1	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	3	0	0	0	0	0	0	0	0	0	0	0	1	0	2	0	1	1	0	0	0
.................................AGCGACCGGAGC-A----------------AAAAG.................................................................................................................................................................................................................	18	1	14.00	14	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	3	1	1	0	0	0	0	0	0	0	0	0	0	0	2	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................................................................................TGGA-AAATAATGGAAAGC.........................................................................................................................................................................	18	1	13.00	13	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	2	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	2	0	0	0
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............................CTCGCAGCGACCGGAGC-A----------------AAAAGTGA..............................................................................................................................................................................................................	26	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
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.................................................................................................................CTGGCCAAGTCG---------------------CAACTAAT...........................................................................................................................	20	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.......................................CGGAGC-A----------------AAAAGTGAGCAGCA........................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.............................TCGCAGCGACCGGAGC-A----------------AAAAGTG...............................................................................................................................................................................................................	24	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.....................................................................GAGCAGCAA----------ATGGA-AAATAATGGAAA...........................................................................................................................................................................	26	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..........................................AGC-A----------------AAAAGTGAGCAGCAA----------AT...........................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.................................AGCGACCGGAGC-A----------------AAAAGATC..............................................................................................................................................................................................................	21	2	0.50	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................ACACTCGCAGCGACCGGAGA-T----------------C.....................................................................................................................................................................................................................	22	2	0.50	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.....................................ACCGGAGC-A----------------AAAAGTGAA.............................................................................................................................................................................................................	18	3	0.33	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0

droEre2

TG--------ACCAGTCGAACAGCGACACTCGCAGCCAGCGGAGC-A----------------ACCAGTGAGC---AA----------GTGGA-AAATAATGGAAAGCC---TCTGGC-AAATCA---------------------CAGCTAATTGAAT---------------------TATTTATAGAAA--ACACT-T-GAGCAGC------TGACATTTAAGTT-CTGGT-TACCCCGTT--------------------------TTGCTG-	Size	Hit Count	Total Norm	Total	M055 Female-body	M057 Embryo	V060 Head	V040 Embryo	V107 Male-body	V108 Head	GSM1528801 follicle cells
......................................................................AGC---AA----------GTGGA-AAATAATG...............................................................................................................................................................................	18	1	2.00	2	0	0	0	0	0	2	0
........................................................................................................................................................................................TATAGAAA--ACACT-T-GAGC.......................................................................	18	1	1.00	1	0	0	0	0	0	1	0
..........................................................................................................................................................................................................................CATTTAAGTT-CTGGT-TACCCCGTT--------------------------TTGCT..	29	1	1.00	1	0	0	0	0	1	0	0
....................................................................................................................................................................................................ACT-T-GAGCAGC------TGACATTTAAGT..................................................	23	1	1.00	1	0	0	0	0	0	1	0
........................................GGAGC-A----------------ACCAGTGAGC---AA----------GTG..........................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	1	0	0
................................................................................................ATAATGGAAAGCC---TCTGGC-AAAT..........................................................................................................................................................	23	1	1.00	1	0	0	0	0	0	1	0
...........................................................................................................................................................................................................AGCAGC------TGACATTTAAGTT-................................................	19	1	1.00	1	0	0	0	0	1	0	0
.......................................................................................................................................................................................TTATAGAAA--ACACT-T-GAGCAGC------TGAC..........................................................	26	1	1.00	1	0	0	0	0	1	0	0
...........................ACTCGCAGCCAGCGGAGC-A----------------ACC...................................................................................................................................................................................................................	22	1	1.00	1	0	0	0	0	1	0	0
.......................................................................................................................................................................................TTATAGAAA--ACACT-T-GAGC.......................................................................	19	1	1.00	1	0	0	0	0	0	1	0
................CGAACAGCGACACTCGCAGCCAGCGGA..........................................................................................................................................................................................................................................	27	1	1.00	1	0	0	0	0	0	1	0
................................................................................................ATAATGGAAAGCC---TCTGGC-A.............................................................................................................................................................	20	1	1.00	1	0	0	0	1	0	0	0
....................................................................................................................................................................................................ACT-T-GAGCAGC------TGACATT.......................................................	18	1	1.00	1	0	0	0	0	0	1	0
.....................................AGCGGAGC-A----------------ACCAGTGAG.............................................................................................................................................................................................................	18	1	1.00	1	1	0	0	0	0	0	0
...............................................................................................AATAATGGAAAGCC---TCTGGC-A.............................................................................................................................................................	21	1	1.00	1	0	0	1	0	0	0	0
............................................................................AA----------GTGGA-AAATAATGGAAAG..........................................................................................................................................................................	20	1	1.00	1	0	0	0	0	1	0	0
................................................................................................................................................................................................................................AGTT-CTGGT-TACCCCGTT--------------------------TTGCT..	23	1	1.00	1	0	0	0	0	1	0	0
........................GACACTCGCAGCCAGCGGA..........................................................................................................................................................................................................................................	19	1	1.00	1	0	0	0	0	0	1	0
...........................................................................................................................................................................................................................ATTTAAGTT-CTGGT-TACCCCGT..................................	22	1	1.00	1	0	0	0	0	1	0	0
.................................AGCCAGCGGAGC-A----------------ACCAGT................................................................................................................................................................................................................	19	1	1.00	1	0	1	0	0	0	0	0

droYak3

TG--------ATCAGTCGAACAGCGACACTCGCAGCAAGCGGACT-A----------------AAAAGTGAGCAGCAA----------GTGGA-AAATAATGGAAATTC---TC---------------------------------AGCTAATTGAAT---------------------TATTTATAGAAA--ATACT-T-CGGCAGC------TGACATTTAAGTG-TTTGC-TACCCCGTTT-------------------------TTGCTG-	Size	Hit Count	Total Norm	Total	M026 Head	M043 Female-body	M056 Embryo	V046 Embryo	V052 Head	V058 Head	V120 Male-body	SRR1275488 Male larvae	SRR1275486 Male prepupae	SRR1275484 Male prepupae	GSM1528802 follicle cells
................CGAACAGCGACACTCGCAGCAAGCGGA..........................................................................................................................................................................................................................................	27	1	6.00	6	0	2	0	0	0	0	4	0	0	0	0
.....................AGCGACACTCGCAGCAAGCGGA..........................................................................................................................................................................................................................................	22	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
..........................................................................GCAA----------GTGGA-AAATAATGG..............................................................................................................................................................................	18	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
.....................AGCGACACTCGCAGCAAGC.............................................................................................................................................................................................................................................	19	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
.....................................................................................................................................................................................................CT-T-CGGCAGC------TGACATTTAAGT..................................................	22	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
..............................................................................................................................................................................................AA--ATACT-T-CGGCAGC------TGA...........................................................	18	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0
................CGAACAGCGACACTCGCAGCAAGCG............................................................................................................................................................................................................................................	25	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
...........................................CT-A----------------AAAAGTGAGCAGCAA----------GTGGA-.......................................................................................................................................................................................	23	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
........................................................................................................................................................................................TATAGAAA--ATACT-T-CGGCAGC------TGA...........................................................	24	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
.....................................................................................................................................................................................ATTTATAGAAA--ATACT-T-CGGCAGC------T.............................................................	25	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
..............................................................................................AAATAATGGAAATTC---TC---------------------------------AGCTAA............................................................................................................................	23	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
.............AGTCGAACAGCGACACTCGCAGCAAG..............................................................................................................................................................................................................................................	26	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
...................................................................................................................................................................................................................................G-TTTGC-TACCCCGTTT-------------------------TTGCT..	21	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
....................................................................................................................................................................................TATTTATAGAAA--ATACT-T-CGGCAGC------T.............................................................	26	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0
........................GACACTCGCAGCAAGCGGAC.........................................................................................................................................................................................................................................	20	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
................CGAACAGCGACACTCGCAGCAAGC.............................................................................................................................................................................................................................................	24	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
................................................................................................ATAATGGAAATTC---TC---------------------------------AGCTAATTGAAT---------------------.................................................................................................	27	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
........................................................................................................................................................................................TATAGAAA--ATACT-T-CGGCAGC------T.............................................................	22	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
.................................AGCAAGCGGACT-A----------------AAAAGTGAGCAGC.........................................................................................................................................................................................................	26	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0
.................GAACAGCGACACTCGCAGCAAGCGGACT-.......................................................................................................................................................................................................................................	28	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
......................................................................................................................................................................................TTTATAGAAA--ATACT-T-CGGCAGC------T.............................................................	24	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
..........................CACTCGCAGCAAGCGGACT-.......................................................................................................................................................................................................................................	19	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
................CGAACAGCGACACTCGCAGCAAGCGGACT-.......................................................................................................................................................................................................................................	29	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
................CGAACAGCGACACTCGCAGCAAGCGGAC.........................................................................................................................................................................................................................................	28	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
...............................................................................................................................................................................................A--ATACT-T-CGGCAGC------TGACATTTAAGT..................................................	26	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0

droEug1

TTAG----CGATCAGTTGAGCAACGACACTCGCAGCGACCGGAGC-T-------AAGCACAAGAAAAGTGAGCAGCAA----------AGGAA-AAATAATGGAAAACC---TCT-CGAAAGTCA---------------------CAACTAATTGAAT---------------------TATTTATAGAAA--ACGCT-T-CAGCAGC------TGACATTTAAGTT-TTTGT-TGCCCTGTTA-------------------------TTGTTG-	Size	Hit Count	Total Norm	Total
No Reads

droBia1

CG--------ATCAGTTGAGCAACGACACTCGCAGCGACCGGAGC-C----------------AAAAGTGAGCAGCAA----------ATGGA-AAATAGTGGAAACCT---TCTGCCAAAGTCACA-------------------AAACTAATTGAAT---------------------TATTTATAGCAA--ACACT-T-CAGCAGCTTGAATTGACATTTGAATT-GTTGT-TGCCCTGTTA-------------------------TTGTTGA	Size	Hit Count	Total Norm	Total
No Reads

droTak1

CG--------ATCAGTTGAGCAACGGCAGTCGCAGCGACCGGAGTGA----------------AAAAGTGAGCAGCAA----------ATGGA-AAATAATGGAAAACC---TCGACCAAAGGCACT-------------------AAACTAATTGAAT---------------------TATTTATAGAAA--ACACT-A-CAGCAGC------TGACATTTGAGTT-TTTGT-TGCCCTGTT---------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droEle1

CG--------ATCAGTTGAGCAACGGCACTCGAAGCGACCGGAGC-C----------------AAAAGTAAGCAGCAA----------ACGGA-AAATAATGGAAAACC---CCAGCCAGTGTCA---------------------CAACTAATTGAAT---------------------TATTTATAGAAAAAACACT-T-CAGCAGC------TGACATTTGAGTT-TTTGT-TGCCCTGTTA-------------------------TTGTTGA	Size	Hit Count	Total Norm	Total
No Reads

droRho1

TG--------ATCAGTTGAGCAACGGCACTCGGAGCGACCGGAGC-C----------------AAAAGTAAGCAACCA----------ATGGA-AAATAATGGAAAACC---TCAGCCAGTGTCA---------------------AAACTAATTGAAT---------------------TATTTATAGAAA--ACATT-T-GAAAAGC------TGACATTTCAGTC-TTTGT-TGCCTTGTTG-------------------------TTGTTGA	Size	Hit Count	Total Norm	Total
No Reads

droFic1

CG--------ATCAGTTGAGCAACGACACTCGAAGCGACCGGAG--A----------------AAAAGTGAGCAGCCA----------GCGGA-AAATAGTGGAAAACC---TCTGCCAAAGTCA---------------------CAACTAATTGAAT---------------------TATTTATAGAAA--ACACT-TCAAGCAGC------TGACATTTAAGTC-ATTGT-TGTCCCGTTT------------------------CTTGTTG-	Size	Hit Count	Total Norm	Total
No Reads

droKik1

TGATTTCGCGATCAGTTGAACAACAACACCCGCGGCGAACGGAGG-C----------------ACCAGTGAGCAGCAA----------GTGGA-AAATAATGGAAAACC---TAGGCAAAAGTCA----------------------AACTAATTGAATT----------TTTTTTTCTGTATTTATAGAAA--ACTCT-T-TCACAGC------TGACATTTAGGTA-TTTGTTTGCTTCGTTG-------------------------TTGTTG-	Size	Hit Count	Total Norm	Total
No Reads

droAna3

TGATTACTCGATCAGTTGGCCTGAGAAGCTCGGAGCAAACGGAGG-C----------------AATAGCTCCCAGCAG----------GCTAT-ATATAATAGAAAACA---GAAGCAACCGTCT-T-------------------ACACCAATTGAAT---------------------TATTTATAGAAT--ATAAT-T-CAGCATC------TGACATTTAAGTTATTTGT-TGCCTTGTTAGAGCCGA---GAACAACAATAAGGTTTATTG-	Size	Hit Count	Total Norm	Total	M044 Female-body	M058 Embryo	V039 Embryo	V055 Head	V105 Male-body	V106 Head
.................GGCCTGAGAAGCTCGGAGC.................................................................................................................................................................................................................................................	19	1	2.00	2	0	0	0	0	0	2
.........................AAGCTCGGAGCAAACGGAGG-C----------------AATAG.................................................................................................................................................................................................................	26	1	1.00	1	0	0	0	1	0	0
........................GAAGCTCGGAGCAAACGGAGG-C----------------AATAGC................................................................................................................................................................................................................	28	1	1.00	1	0	0	0	0	1	0
..........................AGCTCGGAGCAAACGGAGG-C----------------AAT...................................................................................................................................................................................................................	23	1	1.00	1	1	0	0	0	0	0
..............GTTGGCCTGAGAAGCTCGGAGCA................................................................................................................................................................................................................................................	23	1	1.00	1	1	0	0	0	0	0
............................CTCGGAGCAAACGGAGG-C----------------AATAG.................................................................................................................................................................................................................	23	1	1.00	1	1	0	0	0	0	0
...................................................................................................................GCAACCGTCT-T-------------------ACACCAATTGAA.......................................................................................................................	23	1	1.00	1	1	0	0	0	0	0
..............GTTGGCCTGAGAAGCTCGGAT..................................................................................................................................................................................................................................................	21	1	1.00	1	1	0	0	0	0	0

droBip1

TGATTACTCGATCAGTTGGCCAGAGAAACTCGGAGCAACCGGAGG-C----------------AATAGCTCCCAGCAGGCT---ACATATGTACATATAATAGAAAACA---GAAGCAACCGTCT-T-------------------ACACTAATTGAAT---------------------TATTTATAGAGT--ATATT-T-CAGCATC------TGACATTTAAGTTATTTGT-TGCTTTGTTAGAGCCGAGGAGCACAACAACAAGGTTTATTG-	Size	Hit Count	Total Norm	Total
No Reads

dp5

GGAGCAGATCGTCAGTTAAGCAACGGCAGTG------CTTGTGGC-AAGAAGCGAACCT----CGCAGTGGAA-------CACCAGAGACGGA-AAACATTGGAAAATA---TCAA-AAAAGTGG---------------------CCACCAATTGAAT---------------------TATTTATAGAAA--AAACTAT-CAGCAGC------TGACATTTGAGTG--------AATTTGTTT-------------------------GTGGTG-	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
.........................................................CT----CGCAGTGGAA-------CACCAGAGACGGA-AAA....................................................................................................................................................................................	28	1	4.00	4	0	0	0	0	0	0	0	1	3	0
...............................................................CGCAGTGGAA-------CACCAGAGACGGA-A......................................................................................................................................................................................	24	1	2.00	2	0	0	0	0	0	0	0	0	0	2
.................................................................CAGTGGAA-------CACCAGAGACGGA-.......................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	1	0	0	0
.........................................................CT----CGCAGTGGAA-------CACCAGAGACGGA-.......................................................................................................................................................................................	25	1	1.00	1	0	0	0	0	0	0	0	1	0	0
...............................................................CGCAGTGGAA-------CACCAGAGAC...........................................................................................................................................................................................	20	1	1.00	1	0	1	0	0	0	0	0	0	0	0
...............................................................CGCAGTGGAA-------CACCAGAGACGG.........................................................................................................................................................................................	22	1	1.00	1	0	0	0	0	0	0	0	0	1	0

droPer2

GAATCGATCGTTCAGTTAAGCAACGGCAGTG------CTTGTGGC-AAGAAGCGAACCT----CGCAGTGGAA-------CACCAGAGACGGA-AAACATTGGAAAATA---TCAA-AAAAGTGG---------------------CCACCAATTGAAT---------------------TATTTATAGAAA--AAACTAT-CAGCAGC------TGACATTTGAGTG--------AATTTGTTT-------------------------GTGGTG-	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
.......................................................................AA-------CACCAGAGACGGA-AAA....................................................................................................................................................................................	18	1	1.00	1	0	0	0	0	1	0

droWil2

TCAGCG-GCGATCAGTTGTCCAAAGACAGCCG------ACGGAGA-C----------------AGAAGCAAAC---AA----------CGGGA-AACTACTAAAAAAAG---AGAGCAAAAAGCACCAAACGTATAGGAACAGCAACAACAAAT-GCATTAGTAAAGCACATTTTTTCATTATTTATAGAAACAACAAA-T-TTACAGC------TGACATTTGTGTG-TT-----GCTTTGCTGG---------GTATAGTATTTATTTTTGGAG-	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
.......................................................................................................AAAAAG---AGAGCAAAAAGC.........................................................................................................................................................	18	1	1.00	1	0	0	0	1	0
......................................ACGGAGA-C----------------AGAAGCAAAC---AA----------CGGGA-A......................................................................................................................................................................................	26	1	1.00	1	1	0	0	0	0
.......................................................................AC---AA----------CGGGA-AACTACTAA..............................................................................................................................................................................	18	1	1.00	1	0	0	0	1	0
...........................................................................................................................................................................................................................ATTTGTGTG-TT-----GCTTTGCTGG---------GT....................	23	1	1.00	1	0	0	0	1	0
............................................................................................................................ACCAAACGTATAGGAACAGC.....................................................................................................................................	20	1	1.00	1	0	0	0	0	1
......................AAGACAGCCG------ACGGAGA-C----------------A.....................................................................................................................................................................................................................	19	1	1.00	1	0	1	0	0	0
.....................................................................................................................................................................................................AA-T-TTACAGC------TGACATTTGTGT..................................................	22	1	1.00	1	0	0	1	0	0
......................................................................................................AAAAAAG---AGAGCAAAAAGCA........................................................................................................................................................	20	1	1.00	1	0	0	0	0	1
.....................................................................................................AAAAAAAG---AGAGCAAAAA...........................................................................................................................................................	18	9	0.11	1	0	0	0	1	0

dsi_6021

3r:9855102-9855170 +

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads