dsi_29545

TATAGAACCTAAAAAATCAAATTATGACCACCAATCTTTGGGTCGAGCAGGTACGTAATTACACATTGTAAAATGTTTTACAAATAATAATGGCATGTTTTCATAATAGACATAAGTATAAATTTTAAGCTGTCTGCTTAAATATACTGAAAAACAAATTAAAATTTGTTCAAAAGTGTGGGTAGGTGAAAGGAGTGGCATGCCCTCAGTGTTTCAAGTATACAGATAAAAATTGGCAAGGCAAACAATA ************************************************************************************************************************************........((((((....(((((((....)))))))....))))))...*****************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	SRR553488 RT_0-2 hours eggs	SRR553487 NRT_0-2 hours eggs	SRR553486 Makindu_3 day-old ovaries	SRR618934 dsim w501 ovaries	M024 male body	SRR553485 Chicharo_3 day-old ovaries	SRR1275485 Male prepupae
.........................................GTCGAGCAGGTACGAAA................................................................................................................................................................................................	17	1	4	1.00	4	2	2	0	0	0	0	0
.........................................GTCGAGCAGGTACGA..................................................................................................................................................................................................	15	1	7	0.86	6	4	0	2	0	0	0	0
.........................................GTCGAGCAGGTACGAA.................................................................................................................................................................................................	16	1	4	0.75	3	0	2	1	0	0	0	0
.........................................GTCGAGCAGGTACGAAAGTAG............................................................................................................................................................................................	21	3	3	0.67	2	0	1	0	1	0	0	0
.........................................GTCGAGCAGGTACGAAAGT..............................................................................................................................................................................................	19	2	3	0.67	2	1	1	0	0	0	0	0
..........................................TCGAGCAGGTACGAA.................................................................................................................................................................................................	15	1	9	0.56	5	2	2	0	0	0	1	0
.........................................GTCGAGCAGGTACGAAAG...............................................................................................................................................................................................	18	2	5	0.40	2	2	0	0	0	0	0	0
......................................................................................................................................TGCTTAAATATACTGAAAAACAAAT...........................................................................................	25	0	3	0.33	1	0	1	0	0	0	0	0
..........................................TCGAGCAGGTACGAAAGT..............................................................................................................................................................................................	18	2	7	0.29	2	1	1	0	0	0	0	0
..........................................TCGAGCAGGTACGAAA................................................................................................................................................................................................	16	1	7	0.29	2	1	1	0	0	0	0	0
.......................................GGGACGAGCAGGTACGA..................................................................................................................................................................................................	17	2	7	0.29	2	2	0	0	0	0	0	0
.........................................GTCGAGCAGGTACGAAAC...............................................................................................................................................................................................	18	2	5	0.20	1	1	0	0	0	0	0	0
...........................................CGAGCAGGTACGAAAGT..............................................................................................................................................................................................	17	2	13	0.15	2	0	1	0	1	0	0	0
......................................TGGGACGAGCAGGGACG...................................................................................................................................................................................................	17	2	7	0.14	1	1	0	0	0	0	0	0
........................................GGTCGAGCAGGGACGAAA................................................................................................................................................................................................	18	2	8	0.13	1	0	0	0	0	1	0	0
........................................GGTCGATCAGGTACG...................................................................................................................................................................................................	15	1	9	0.11	1	0	1	0	0	0	0	0
...........................................CGAGCAGGTACGAAA................................................................................................................................................................................................	15	1	9	0.11	1	1	0	0	0	0	0	0
......................................TGGGACGAGCAGGGAC....................................................................................................................................................................................................	16	2	20	0.10	2	2	0	0	0	0	0	0
........................................CGTCGAGCAGGTACGAA.................................................................................................................................................................................................	17	2	10	0.10	1	1	0	0	0	0	0	0
.......................................................................................................................................................................GTTCAAAAGTGTGGGT...................................................................	16	0	12	0.08	1	0	0	0	1	0	0	0
.........................................GTCGATCAGGTACGAAA................................................................................................................................................................................................	17	2	13	0.08	1	1	0	0	0	0	0	0
......................................CGGCTCGAGCAGGTACGAA.................................................................................................................................................................................................	19	3	13	0.08	1	0	0	1	0	0	0	0
.....................................ATGGGTCGAGCAGGGACGA..................................................................................................................................................................................................	19	3	14	0.07	1	1	0	0	0	0	0	0
.........................................GTCGACCAGGTACGAAAGT..............................................................................................................................................................................................	19	3	14	0.07	1	1	0	0	0	0	0	0
..........................................TCGAGCAGGTACGAAAG...............................................................................................................................................................................................	17	2	18	0.06	1	0	0	1	0	0	0	0
............................................................................................................................................................................................AAGGGAAGGGCATGCCCTC...........................................	19	3	18	0.06	1	0	0	0	1	0	0	0
...............................................................................................................................................................................GTGTGGGTAGGGGAA............................................................	15	1	19	0.05	1	0	0	0	0	0	0	1
......................................TGGGACGAGCAGGGACGAA.................................................................................................................................................................................................	19	3	19	0.05	1	1	0	0	0	0	0	0
.........................................TTCGAGCAGGTACGAA.................................................................................................................................................................................................	16	2	20	0.05	1	1	0	0	0	0	0	0

ATATCTTGGATTTTTTAGTTTAATACTGGTGGTTAGAAACCCAGCTCGTCCATGCATTAATGTGTAACATTTTACAAAATGTTTATTATTACCGTACAAAAGTATTATCTGTATTCATATTTAAAATTCGACAGACGAATTTATATGACTTTTTGTTTAATTTTAAACAAGTTTTCACACCCATCCACTTTCCTCACCGTACGGGAGTCACAAAGTTCATATGTCTATTTTTAACCGTTCCGTTTGTTAT *****************************************************************........((((((....(((((((....)))))))....))))))...************************************************************************************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	SRR553486 Makindu_3 day-old ovaries	SRR553487 NRT_0-2 hours eggs	SRR553485 Chicharo_3 day-old ovaries
.........................................................................................................................................................................................................CGGGAGTCACAAAGTTCATATGTCTATT.....................	28	0	3	0.33	1	0	1	0
.........................................................................................................................................................................................................CGGAAGTCACAAAGTTCATATGTCTATTT....................	29	1	3	0.33	1	1	0	0
........................................................................................................................................................................................................ACGGGAGTCACAAAGTTCATATGTCTAT......................	28	0	3	0.33	1	0	0	1
..................................................................................................................................................................TTAAACAAGTTTTCACACCCATCCAC..............................................................	26	0	3	0.33	1	1	0	0
...................................GAAGCCCAGGTCGTCCAT.....................................................................................................................................................................................................	18	2	7	0.14	1	0	1	0
.................................................................................................................................................................GTTAAACAAGTTTTCACAGCC....................................................................	21	2	11	0.09	1	0	0	1

Species	Coordinate	ID	Alignment
droSim2	3l:22720169-22720418 +	dsi_29545	TATAGAACCTAAAAAATCAAATTATGACCACCAATCTTTGG---------GTCGAGCAGG-----TACGTAATTACACATTGTAAAATGT-----TTTA--CAAATA-ATAA------------------TGGCATGTTTT--------------------CATAATAGACATAAG---------TATAAA-------------------------------------------------------------------------------------------------TTTTAAG----------------------------------------------------------------CTGTCTGCTTAAATATACTGAAAAACAAATTAAAATTTGTTCAAAAGTGTGG----------------------GTAGGTGAAAGGAGTGGCATGCCCTCAGTGTTTCAAGTATAC---------------------------------------------AGATAAAAATTGGCAA-G-------------------------------------------------------------------------------------------------------GCAAACAATA
droSec2	scaffold_41:134337-134585 +		TATAGAACCTAAAAAATCAAATTATGACCACCAATCTTTGG---------GTTGAGCAGG-----TACGTAATTACACATTGTAAAATGT-----TTCA--CAAATA-ATAA------------------TGGCATGTTTT--------------------CGTAATAGACATAAG---------TATAAA-------------------------------------------------------------------------------------------------TTCTAAG----------------------------------------------------------------CTGTCTGCTTAAATAAAATGAAAAA-AAAATAAAATTTGTTCAAAAGTGTGG----------------------GTTGGTGAAAGGAGTGGCATGCCCTCAGTGTTTCAAGTATAG---------------------------------------------AGATAAAAATTGGCAA-G-------------------------------------------------------------------------------------------------------GCAAACAATA
dm3	chr3L:23209159-23209507 +		T-TAGAACCTAAAAAATCAAATTATGACCACCAATCTTTGG---------GTCGAGCAGG-----TACGTAATTACACATTGTAAAATGT-----TTAA--CAAATA-ATAA------------------TGGCATAATTT--------------------ATTGATAGACATAAG---------TAGAAATTGT-ACTAA---------------------------------------------GG------------------------------------------CAAAG----------------------------------------------------------------ATGTCTGCTTAAATAAAATGAATAAAAACTAAAAAATTGTTCAAAAGTGTGCGCGTGACCGGGTTGGGCGTTTTGTAGATGAAAGGAGTGGCATGCCCTCAGTGTTTCTGGTATACAGAAAAAGCTAA-AAATTTTTCAAAGGTTTGGACTTGGCAGCTTGGGAT-----G--------------------TTTTGTGAGCGTGGCAAAAAGTTTT---TG------------------------------------------------AGAAATTTAGAGGACT---AATA
droEre2	scaffold_4845:22290224-22290478 +		CTTGAGATATGGAG-------TTTTGAACTTCAGTCTTTGG---------ACATTTCTGAATTACGACAACATCATACATCAGA----------------------------------------------------------------------------------------------------------------------------------------------------------------AAATTGACAAAAAAATTTAAAA------AAAATATATTTCTAAATTTGAAT--------------GCACATTTGTAGAGAATTTCGGCAGAAACGTTTAG---------------AGGTATGCCACTTT-----------------------------------------------------------------------------------------------------------------------------AAAATCCGATCAAGATATCTTGAGATATGGAG--------------------TTTTGAGGGCGTGGCAAAATGTTTT---TC-------------------TGCAAGTC------------------TATAGAAATTTACAAGACTAATAAAA
droYak3	v2_chr2h_random_005:83043-83215 -		CGAATGGTAA---------------------------------------------------------CGTAAATGCTTATA----------------------------------------------------------------------------------------------------------TAAA----------------------------------------------------TGCGTTGATAA-----------------------------------TTAAC------------------------------------------------------------------------------AATATAACAAGAGAAATTGCTT----------------------------------------------------------AA---------------------------GC-TTATAAGCTATTCGTA-GT---------------------GAAAATGA-AAA-GGAGAAATTTCAACACTGTGGGCGTGCCAAAACCTTAT---TC-------------------TGCAAATC------------------GATAAAAATTTAAAAAACTAATACAA
droEug1	scf7180000409551:270135-270356 -		TATACAACTTATTATAGCGAAGTTTTAATACAG--TTTTGG---------GACAACCTCA-----TTATTCATGGTGTTTCTAAAAGTAT-----GCTA--CAAA-----------------------------------------------------------------------AATATCGGT---------------------------------------------------AACTGTG---------AAGTAG-AATTGCCATGTTGTAGAAAAAAGTCGACAATTTAAA--------------TC--------------------------------------------------AAATTTTACAATGCTTT---------------------CCAAAA-----------------------------------------------------------------------------------------------------------------------ATAAATCA-AATTGCAC--------------------------TTAATTGT---CTAACTAGTTTAGAAAAATGT-------TATCT---------------------------------TAATGTT
droBia1	scf7180000300279:7450-7588 +		ACAGGTTTA------------------------------------------------------------------------------------AA------------------------------------------------------------------------------------------TCTAGA-------------------------------------------------------------------------------------------------TACT--G----------------------------------------------------------------ATGGTCACTTAAATGTTCAAAAAAAAGAATTAAAAACTCTTCAAAACCA-------------------------ATAAATG---GAAATGATTTG---------------------------GC-TAATAAATTTTATGTAG-------------------ATGAGAAAATTAACAA-AGCGAA---------------------------------------------------------------------------------------------------ATCACAACA
droTak1	scf7180000415956:54770-54942 -		TATAGAACCTGAAAAATCAAATAATGACTACAAATCTTTGG---------GTTGAGCAGG-----TAAGTAATGTA---TTGTTTTAAATGAAATTTTTGGTGTACAATTTAA--------------------CTCGTTTTCGTAAGTCAAATTTGGTACTCT---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GATTTG---------------------------AGCTTA-AAATTTTTTATAGGT---------------------GCAAATAA-AAAGAGTG----------------------------------------------------------------------------------------------------------TCAT
droEle1	scf7180000491238:888866-889019 +		TTGAAAAAA------------------------------------------------------------------------------------ATTTTA--CGTATA-ATTAA--------------------TATACATACACAGATTCAATCAAGTTCTTTGTATCGGTAAAGA---------AGTTAAAATTCACTAAAAAAATTGCTATTAAAAAAAAAGGAATATCAATTC----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AGTCTAG---------------------------------------------AGATAAAGTGTCTTAA-G-------------------------------------------------------------------------------------------------------CCAAATAAGA
droRho1	scf7180000779479:473832-473988 +		TAAAAAATTAAAATAAATAT--------TTTCAGAGGCTGG---------ATCAA----A-----TTTGTAATTTTATTTAAAAAAATCA-----TTCA--T--T-----------------------------------------------------------------------ATTATAGAT---------------------------------------------------TATTGTG---------ATATAT-TTTTTAA------TTAACACATTTGTATATTTTAAA--------------AC--------------------------------------------------ATATTCTTAGATATATTTTGAAA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AGATTTGAAA
droFic1	scf7180000453798:35783-35935 -		TACAGAACCTTAAAAATCAAATAATGACTACCAATCTTTGG---------GTTGAGCAGG-----TAAGTGAC-GTATATAGTTTTATGT------------TAACA-TTTA------------------CATTTAGTTTT--------------------TATTACTTTCTAAAA---------ATTTAAT------------------------------------------------------------------------------------------------TTATACG----------------------------------------------------------------CAGTAAACT--------ATAAAAAATACAATA---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AAATT
droKik1	scf7180000302387:140863-140995 -		TGCAGAATCTGAAGAATCAAATCATGACAACCAATGTCTGG---------GTGGAGCAGG-----TAAGCTT---------------------------------------------------------------------------------------------------------------------------CACTTTA-AATATA-------------------------------------------------------------------------------AAGCATGATATATAGTATGGTGCATATA-------------------------TATAAGTATATATTTGTGCGGTTTGCTCA------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ACTGT
droAna3	scaffold_13266:19334488-19334684 +		AAATACGTA------------------------------------------------------------------------------------ATTTTG--TAAATA-TATATGTGAAGCATTTTACTGCTGCCATCTAAT------------CGT-----ATTCCTAAA--TATG---------TATAAA-------------------------------------------------------------------------------------------------AATTATG----------------------------------------------------------------ATATATAAAAATATATAATATAAAATATAATAAAATTTTTTCACAGGTATAT----------------------AATAATGATA---------------------------------------------------------------------------------------------------ACTCCCATTTTCTTAGGGTA------------------------------------------------GCATGCCTGTAAAGTTACAAAA---------ATCAAAAATT
droBip1	scf7180000396535:1210452-1210560 -		TGTATATTTT-------------------------------------------------------------------------------------TTCA--CAAATT-GTTA------------------AATCCAGTTCA--------------------TGTGATTGTCACAAT---------AATAAACTTTTAC----------------------------------------------ACGTTGAG----------------------------------------------------------------------------------------------------------------AACG---AAATACAAATAAAAAAATTGAAAATTATTCAAA------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------A
dp5	Unknown_group_7:135359-135411 +		TATAGAACTTAAAAAATCAAATAATGACTACCAATCTTTGG---------GTGGAGCAGG-----TA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droPer2	scaffold_3:1293033-1293100 +		TGCAGAACTTGAAGAATCAAATTATGACCACCAATGTGTGG---------GTGGAGCAGG-----TAAGCTT---------------------------------------------------------------------------------------------------------------------------AACTAT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AAAT
droWil2	scf2_1100000004921:4461596-4461729 +		TTCAGAATTTAAAAAATCAAATTATGACTACCAATGTATGG---------GTGGAGCAGG-----TAAGTGTT---------------------------------------------------------------------------------------------------------------------------ATCAATCAATACACAAACACAAATAAAATAATAATAATAAATTTAAG---------AAA---------------------------------TTTAAAT----------------------------------------------------------------GAATTTGCTCA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ACTGTAC
droVir3	scaffold_13047:3591774-3591853 -		TGCAGAATCTAAAAAATCAAATTATGACTACCAACGTTTGG---------GTGGAGCAGG-----TGAGTCA---------------------------------------------------------------------------------------------------------------------------AACTT-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TCCCATTTT-------------------TGTA--------------------------------------------AAAT
droMoj3	scaffold_6540:4512837-4512936 +		CACGCTGCCACAAAAAACATATA------------------------------------------------------------------------------------------------------------------------------------------------------------------CATAAG----------------------------------------------------TGCGATGGG----------------------------------------------------------------------------------------------------------------TGCGTA--TACACAGAAAAACAAATTGCCTTTTGTTGCAAAATGTG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GCAAGACGGA----------------------GGA-GCATG
droGri2	scaffold_15126:840340-840448 +		ACCAATTTT-----------------------------TCGCAAAAGGGAAACGAGGAAG-----CAAATAATTACACATTTTTGTATGT-----TTGA--CAAAT---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTAAAT----------------------------------------------------------------------------------------AAATATTGCTTTTCTATGAAAA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AGA-------TGTGTA--------------------------------AATATTT

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droSim2

TATAGAACCTAAAAAATCAAATTATGACCACCAATCTTTGG---------GTCGAGCAGG-----TACGTAATTACACATTGTAAAATGT-----TTTA--CAAATA-ATAA------------------TGGCATGTTTT--------------------CATAATAGACATAAG---------TATAAA-------------------------------------------------------------------------------------------------TTTTAAG----------------------------------------------------------------CTGTCTGCTTAAATATACTGAAAAACAAATTAAAATTTGTTCAAAAGTGTGG----------------------GTAGGTGAAAGGAGTGGCATGCCCTCAGTGTTTCAAGTATAC---------------------------------------------AGATAAAAATTGGCAA-G-------------------------------------------------------------------------------------------------------GCAAACAATA	Size	Hit Count	Total Norm	Total	M023 Head	M024 Male-body	M025 Embryo	M053 Female-body	O001 Testis	O002 Head	SRR553485 Ovary	SRR553486 Ovary	SRR553487 Ovary	SRR553488 Ovary	SRR618934 Ovary	V044 Embryo	SRR902008 Ovary	SRR902009 Testis	SRR1275487 Male larvae	SRR1275485 Male prepupae	SRR1275483 Male prepupae
..................................................GTCGAGCAGG-----TACGA.....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	15	7	0.86	6	0	0	0	0	0	0	0	2	0	4	0	0	0	0	0	0	0
............................................................................................................................................................................................................................................................................................................................................................................TGCTTAAATATACTGAAAAACAAAT......................................................................................................................................................................................................................................................................	25	3	0.33	1	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0
.............................................................................................................................................................................................................................................................................................................................................................................................................GTTCAAAAGTGTGG----------------------GT........................................................................................................................................................................................................................	16	12	0.08	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0

droSec2

TATAGAACCTAAAAAATCAAATTATGACCACCAATCTTTGG---------GTTGAGCAGG-----TACGTAATTACACATTGTAAAATGT-----TTCA--CAAATA-ATAA------------------TGGCATGTTTT--------------------CGTAATAGACATAAG---------TATAAA-------------------------------------------------------------------------------------------------TTCTAAG----------------------------------------------------------------CTGTCTGCTTAAATAAAATGAAAAA-AAAATAAAATTTGTTCAAAAGTGTGG----------------------GTTGGTGAAAGGAGTGGCATGCCCTCAGTGTTTCAAGTATAG---------------------------------------------AGATAAAAATTGGCAA-G-------------------------------------------------------------------------------------------------------GCAAACAATA	Size	Hit Count	Total Norm	Total	M054 Female-body	V113 Male-body	V114 Embryo	V115 Head
No Reads

dm3

T-TAGAACCTAAAAAATCAAATTATGACCACCAATCTTTGG---------GTCGAGCAGG-----TACGTAATTACACATTGTAAAATGT-----TTAA--CAAATA-ATAA------------------TGGCATAATTT--------------------ATTGATAGACATAAG---------TAGAAATTGT-ACTAA---------------------------------------------GG------------------------------------------CAAAG----------------------------------------------------------------ATGTCTGCTTAAATAAAATGAATAAAAACTAAAAAATTGTTCAAAAGTGTGCGCGTGACCGGGTTGGGCGTTTTGTAGATGAAAGGAGTGGCATGCCCTCAGTGTTTCTGGTATACAGAAAAAGCTAA-AAATTTTTCAAAGGTTTGGACTTGGCAGCTTGGGAT-----G--------------------TTTTGTGAGCGTGGCAAAAAGTTTT---TG------------------------------------------------AGAAATTTAGAGGACT---AATA	Size	Hit Count	Total Norm	Total	GSM154618 Ovary	GSM154620 Ovary	GSM154621 Ovary	GSM154622 Ovary	GSM180328 Head	GSM180329 Mixed-body	GSM180330 Embryo	GSM180331 Embryo	GSM180332 Embryo	GSM180333 Embryo	GSM180334 Larvae	GSM180335 Disc	GSM180336 Pupae	GSM180337 S2	GSM231091 Ovary	GSM239041 Head	GSM239050 Head	GSM239051 S2	GSM239052 S2	GSM246084 Head	GSM266765 S2	GSM280082 Ovary	GSM280083 Ovary	GSM280084 Ovary	GSM280085 Testis	GSM280086 Ovary	GSM280087 S2-NP	GSM280088 S2-NP	GSM312995 Head	GSM313160 Head	GSM313161 Head	GSM313162 Head	GSM313163 Head	GSM313164 Head	GSM313165 Head	GSM343287 Embryo	GSM379050 Ovary	GSM379051 Ovary	GSM379052 Ovary	GSM379053 Ovary	GSM379054 Ovary	GSM379055 Ovary	GSM379056 Ovary	GSM379057 Ovary	GSM379058 Ovary	GSM379059 Ovary	GSM379060 Ovary	GSM379061 Ovary	GSM379062 Ovary	GSM379063 Ovary	GSM379064 Ovary	GSM379065 Ovary	GSM379066 Ovary	GSM379067 Ovary	GSM424739 S2	GSM424740 S2	GSM424741 S2	GSM467729 Female-body	GSM467730 Female-body	GSM467731 Female-body	M002 CS	M003 Male-body	M004 Male-body	M005 ML-DmD32	M006 ML-DmD32	M029 OSC	M030 OSC	M031 OSC	M032 S2-R+	M033 S2-R+	M034 S2-R+	M035 S2-R+	M036 S2-R+	M037 S2-R+	M038 S2-R+	M039 S2-R+	M048 S2-R+	OSS2 OSS	OSS6 OSS	OSS7 OSS	OSS8 OSS	S1 Head	S10 Embryo	S11 Embryo	S12 Embryo	S13 Embryo	S14 Embryo	S15 S2-GMR	S16 Kc	S17 Larvae	S18 Larvae	S19 Pupae	S2 Head	S20 Pupae	S21 Disc	S22 Head	S23 Female-body	S24 Male-body	S25 Larvae	S26 Larvae	S27 Pupae	S28 Pupae	S29 Head	S3 Male-body	S30 S2-GMR	S31 S2	S32 S2	S33 Kc	S34 Kc	S35 Kc	S4 Female-body	S5 Disc	S6 Embryo	S7 Embryo	S8 S2	S9 Embryo	SRR001337 Head	SRR001338 Head	SRR001339 Head	SRR001340 Head	SRR001341 Head	SRR001342 Head	SRR001343 Head	SRR001344 Head	SRR001345 Head	SRR001346 Head	SRR001347 Head	SRR001348 Head	SRR001349 Head	SRR001664 Head	SRR010951 Ovary	SRR010952 Ovary	SRR010953 Ovary	SRR010954 Ovary	SRR010955 Ovary	SRR010956 Ovary	SRR010957 Ovary	SRR010958 Ovary	SRR010959 Ovary	SRR010960 Ovary	SRR014268 Embryo	SRR014269 Embryo	SRR014270 Embryo	SRR014271 Embryo	SRR014272 Embryo	SRR014273 Ovary	SRR014274 Ovary	SRR014275 Ovary	SRR014276 Ovary	SRR014277 Ovary	SRR014278 Ovary	SRR014279 Ovary	SRR014280 Ovary	SRR014281 Ovary	SRR014282 Ovary	SRR023197 S2	SRR023399 S2	SRR023400 S2	SRR023402 S2	SRR023407 S2	SRR029028 S2	SRR029029 S2	SRR029030 S2	SRR029031 S2	SRR029032 S2	SRR029033 S2	SRR029608 Head	SRR029633 Head	SRR030145 gb3	SRR031692 Head	SRR031693 Head	SRR031694 Head	SRR031696 Head	SRR031697 Head	SRR031698 Head	SRR031699 Head	SRR031700 Head	SRR031701 Head	SRR031702 Head	SRR031703 Head	SRR031704 Head	SRR032092 S2	SRR032093 S2	SRR032094 S2	SRR032095 S2-NP	SRR032096 S2-NP	SRR032152 Head	SRR060643 Testis	SRR060644 Ovary	SRR060645 Testis	SRR060646 Ovary	SRR060647 Ovary	SRR060648 Ovary	SRR060649 Ovary	SRR060650 Testis	SRR060651 Ovary	SRR060652 Testis	SRR060653 Ovary	SRR065151 Ovary	SRR065152 Ovary	SRR065153 Ovary	SRR065154 Ovary	SRR065155 Ovary	SRR065800 Ovary	SRR065801 Ovary	SRR065802 Ovary	SRR065803 Ovary	SRR065804 Ovary	SRR065805 Ovary	SRR065806 Ovary	SRR065807 Ovary	SRR097865 S2-NP	SRR097866 S2-NP	SRR097867 S2-NP	SRR298536 Ovary	SRR298537 Ovary	SRR298711 Ovary	SRR298712 Ovary	SRR317110 Ovary	SRR317111 Ovary	SRR317112 Ovary	SRR317113 Ovary	SRR317114 Ovary	SRR317115 Ovary	SRR317116 Ovary	SRR317117 Ovary	SRR341115 S2	SRR341116 S2	SRR341117 S2	SRR341118 S2	SRR351332 Mixed-body	SRR351333 Mixed-body	T004 OSC	T005 CME-L1	V001 Embryo	V002 Embryo	V003 S2	V006 Female-body	V007 Head	V008 S2	V009 CME-L1	V010 ML-DmD20-C5	V011 Sg4	V012 Male-body	V013 Head	V014 DTT	V015 DreRFHV	V018 Kc167	V019 GM2	V020 S2-R+	V021 ML-DmD21	V022 ML-DmD32	V023 Head	V024 Kc167	V025 S2-R+	V026 1182-2H	V027 ML-DmD21	V028 CME-W1-Cl.8+	V029 ML-DmD9	V030 ML-DmD8	V031 GM2	V032 S1	V033 CME-W1-Cl.8+	V034 ML-DmD16-C3	V035 ML-DmD32	V036 ML-DmD20-C5	V037 S2	V038 S2	V063 Ovary	V0632 Ovary	V064 Ovary	V0642 Ovary	V065 Ovary	V0652 Ovary	V066 Ovary	V0662 Ovary	V067 S2-NP	V073 mbn2	V074 S3	V075 ML-DmBG1-C1	V076 ML-DmBG3-C2	V077 Head	V078 Head	V079 Head	V080 Head	V081 Embryo	V082 Embryo	V083 Male-body	V084 Female-body	V085 CME-W2	V086 Female-body	V088 Ovary	V0882 Ovary	V089 Ovary	V0892 Ovary	V090 Male-body	V091 fGS/OSS	V092 Male-body	V093 Male-body	V094 S2-R+	V095 S2-R+	V096 Ovary	V097 Ovary	V098 Ovary	V125 ML-DmD9	V126 CME-L1	V127 G2	V128 S3	V129 ML-DmBG1-C1	V130 ML-DmBG3-C2	V131 ML-DmD16-C3	V132 ML-DmD32	V133 Sg4	V134 ML-DmD8	V135 CME-W2	V136 Male-body	V137 Head	V138 Male-body	V139 Female-body	V140 Female-body	V141 Female-body	V142 Female-body	V144 OSC	V145 S2	V146 S1	V147 1182-2H	V148 mbn2	GSM1528798 follicle cells
..................................TCTTTGG---------GTCGAGCAGG-----TACGTAAT..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	25	1	5.00	5	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	5	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...............................................................................................................................................................................................TTGT-ACTAA---------------------------------------------GG------------------------------------------CAAAG----------------------------------------------------------------ATGTCTGC............................................................................................................................................................................................................................................................................................	24	1	2.00	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TTGGGAT-----G--------------------TTTTGTGAGCGTGGCAAA...................................................................................	26	1	2.00	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GAT-----G--------------------TTTTGTGAGCGTGGCAAAAAGT...............................................................................	26	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..............................................................................................................................................................................................................................................................................................................................................................................................................................................TTTGTAGATGAAAGGAGTGGCATGCCC..................................................................................................................................................................................................	27	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................................................................................................................................ATTT--------------------ATTGATAGACATAA............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	18	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
........................................................CAGG-----TACGTAATTACACATTGTAAAA....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	26	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
......................................................................................................................................................................................................................................................................................................................................................................................................................................TTGGGCGTTTTGTAGATGAAAGGAGT...........................................................................................................................................................................................................	26	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
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..................................................................................................................................................................................................................................................................................................................................................................................................................................................TAGATGAAAGGAGTGGCATGC....................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ATTTTTCAAAGGTTTGGACT.............................................................................................................................................	20	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ACTTGGCAGCTTGGGAT-----G--------------------TTT..................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
......................................................................................................................................................................................................................................................................................................................................................................................................................................TTGGGCGTTTTGTAGATGAAAGGA.............................................................................................................................................................................................................	24	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
............................................................................................................................................................................TAAG---------TAGAAATTGT-ACTAA---------------------------------------------GG------------------------------------------C........................................................................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...............................................................................................................................................................................................................................................................................................................................................................................................................................................TTGTAGATGAAAGGAGTGGCATGCCCTC................................................................................................................................................................................................	28	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0
.............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TTTCAAAGGTTTGGACTTGGCAGC......................................................................................................................................	24	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0
........................................................................................................................................................................................................A---------------------------------------------GG------------------------------------------CAAAG----------------------------------------------------------------ATGTCTGCTT..........................................................................................................................................................................................................................................................................................	18	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

droEre2

CTTGAGATATGGAG-------TTTTGAACTTCAGTCTTTGG---------ACATTTCTGAATTACGACAACATCATACATCAGA----------------------------------------------------------------------------------------------------------------------------------------------------------------AAATTGACAAAAAAATTTAAAA------AAAATATATTTCTAAATTTGAAT--------------GCACATTTGTAGAGAATTTCGGCAGAAACGTTTAG---------------AGGTATGCCACTTT-----------------------------------------------------------------------------------------------------------------------------AAAATCCGATCAAGATATCTTGAGATATGGAG--------------------TTTTGAGGGCGTGGCAAAATGTTTT---TC-------------------TGCAAGTC------------------TATAGAAATTTACAAGACTAATAAAA	Size	Hit Count	Total Norm	Total	M055 Female-body	M057 Embryo	V060 Head	V040 Embryo	V107 Male-body	V108 Head	GSM1528801 follicle cells
No Reads

droYak3

CGAATGGTAA---------------------------------------------------------CGTAAATGCTTATA----------------------------------------------------------------------------------------------------------TAAA----------------------------------------------------TGCGTTGATAA-----------------------------------TTAAC------------------------------------------------------------------------------AATATAACAAGAGAAATTGCTT----------------------------------------------------------AA---------------------------GC-TTATAAGCTATTCGTA-GT---------------------GAAAATGA-AAA-GGAGAAATTTCAACACTGTGGGCGTGCCAAAACCTTAT---TC-------------------TGCAAATC------------------GATAAAAATTTAAAAAACTAATACAA

Size

Hit Count

Total Norm

Total

M026
Head

M043
Female-body

M056
Embryo

V046
Embryo

V052
Head

V058
Head

V120
Male-body

SRR1275488
Male larvae

SRR1275486
Male prepupae

SRR1275484
Male prepupae

GSM1528802
follicle cells

No Reads

droEug1

TATACAACTTATTATAGCGAAGTTTTAATACAG--TTTTGG---------GACAACCTCA-----TTATTCATGGTGTTTCTAAAAGTAT-----GCTA--CAAA-----------------------------------------------------------------------AATATCGGT---------------------------------------------------AACTGTG---------AAGTAG-AATTGCCATGTTGTAGAAAAAAGTCGACAATTTAAA--------------TC--------------------------------------------------AAATTTTACAATGCTTT---------------------CCAAAA-----------------------------------------------------------------------------------------------------------------------ATAAATCA-AATTGCAC--------------------------TTAATTGT---CTAACTAGTTTAGAAAAATGT-------TATCT---------------------------------TAATGTT	Size	Hit Count	Total Norm	Total
No Reads

droBia1

ACAGGTTTA------------------------------------------------------------------------------------AA------------------------------------------------------------------------------------------TCTAGA-------------------------------------------------------------------------------------------------TACT--G----------------------------------------------------------------ATGGTCACTTAAATGTTCAAAAAAAAGAATTAAAAACTCTTCAAAACCA-------------------------ATAAATG---GAAATGATTTG---------------------------GC-TAATAAATTTTATGTAG-------------------ATGAGAAAATTAACAA-AGCGAA---------------------------------------------------------------------------------------------------ATCACAACA	Size	Hit Count	Total Norm	Total
No Reads

droTak1

TATAGAACCTGAAAAATCAAATAATGACTACAAATCTTTGG---------GTTGAGCAGG-----TAAGTAATGTA---TTGTTTTAAATGAAATTTTTGGTGTACAATTTAA--------------------CTCGTTTTCGTAAGTCAAATTTGGTACTCT---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GATTTG---------------------------AGCTTA-AAATTTTTTATAGGT---------------------GCAAATAA-AAAGAGTG----------------------------------------------------------------------------------------------------------TCAT	Size	Hit Count	Total Norm	Total
No Reads

droEle1

TTGAAAAAA------------------------------------------------------------------------------------ATTTTA--CGTATA-ATTAA--------------------TATACATACACAGATTCAATCAAGTTCTTTGTATCGGTAAAGA---------AGTTAAAATTCACTAAAAAAATTGCTATTAAAAAAAAAGGAATATCAATTC----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AGTCTAG---------------------------------------------AGATAAAGTGTCTTAA-G-------------------------------------------------------------------------------------------------------CCAAATAAGA	Size	Hit Count	Total Norm	Total
No Reads

droRho1

TAAAAAATTAAAATAAATAT--------TTTCAGAGGCTGG---------ATCAA----A-----TTTGTAATTTTATTTAAAAAAATCA-----TTCA--T--T-----------------------------------------------------------------------ATTATAGAT---------------------------------------------------TATTGTG---------ATATAT-TTTTTAA------TTAACACATTTGTATATTTTAAA--------------AC--------------------------------------------------ATATTCTTAGATATATTTTGAAA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AGATTTGAAA	Size	Hit Count	Total Norm	Total
No Reads

droFic1

TACAGAACCTTAAAAATCAAATAATGACTACCAATCTTTGG---------GTTGAGCAGG-----TAAGTGAC-GTATATAGTTTTATGT------------TAACA-TTTA------------------CATTTAGTTTT--------------------TATTACTTTCTAAAA---------ATTTAAT------------------------------------------------------------------------------------------------TTATACG----------------------------------------------------------------CAGTAAACT--------ATAAAAAATACAATA---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AAATT	Size	Hit Count	Total Norm	Total
No Reads

droKik1

TGCAGAATCTGAAGAATCAAATCATGACAACCAATGTCTGG---------GTGGAGCAGG-----TAAGCTT---------------------------------------------------------------------------------------------------------------------------CACTTTA-AATATA-------------------------------------------------------------------------------AAGCATGATATATAGTATGGTGCATATA-------------------------TATAAGTATATATTTGTGCGGTTTGCTCA------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ACTGT	Size	Hit Count	Total Norm	Total
No Reads

droAna3

AAATACGTA------------------------------------------------------------------------------------ATTTTG--TAAATA-TATATGTGAAGCATTTTACTGCTGCCATCTAAT------------CGT-----ATTCCTAAA--TATG---------TATAAA-------------------------------------------------------------------------------------------------AATTATG----------------------------------------------------------------ATATATAAAAATATATAATATAAAATATAATAAAATTTTTTCACAGGTATAT----------------------AATAATGATA---------------------------------------------------------------------------------------------------ACTCCCATTTTCTTAGGGTA------------------------------------------------GCATGCCTGTAAAGTTACAAAA---------ATCAAAAATT	Size	Hit Count	Total Norm	Total	M044 Female-body	M058 Embryo	V039 Embryo	V055 Head	V105 Male-body	V106 Head
....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ATTTTCTTAGGGTA------------------------------------------------GCATGCC..................................	21	20	22.35	447	0	1	0	0	0	446
...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CATTTTCTTAGGGTA------------------------------------------------GCATGCC..................................	22	20	4.75	95	55	0	0	0	6	34
...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CATTTTCTTAGGGTA------------------------------------------------GCATGC...................................	21	20	4.75	95	63	2	0	0	30	0
.........................................................................................................................TTTTACTGCTGCCATCTAAT------------C.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	21	20	3.05	61	61	0	0	0	0	0
...........................................................................................................................TTACTGCTGCCATCTAAT------------CG................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	20	20	1.85	37	30	0	0	1	0	6
...........................................................................................................................TTACTGCTGCCATCTAAT------------CGT-----..........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	21	20	1.55	31	0	0	0	2	0	29
..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CCATTTTCTTAGGGTA------------------------------------------------GCATG....................................	21	20	1.50	30	0	0	0	0	19	11
..........................................................................................................................TTTACTGCTGCCATCTAAT------------CG................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	21	20	1.35	27	26	0	0	0	0	1
...........................................................................................................................TTACTGCTGCCATCTAAT------------CGT-----A.........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	22	20	1.30	26	24	0	0	0	0	2
.........................................................................................................................TTTTACTGCTGCCATCTAAT------------CG................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	22	20	0.65	13	12	0	0	0	0	1
...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CATTTTCTTAGGGTA------------------------------------------------GCATG....................................	20	20	0.30	6	6	0	0	0	0	0
......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TTTCTTAGGGTA------------------------------------------------GCATGCCTG................................	21	20	0.25	5	0	0	0	4	0	1
........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TCTTAGGGTA------------------------------------------------GCATGCCTGTA..............................	21	20	0.25	5	5	0	0	0	0	0
...........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TAGGGTA------------------------------------------------GCATGCCTGTAAAG...........................	21	20	0.20	4	3	0	0	1	0	0
...............................................................................................................................TGCTGCCATCTAAT------------CGT-----ATTCC.....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	22	20	0.20	4	2	0	0	0	0	2
................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TA------------------------------------------------GCATGCCTGTAAAGTTACA......................	21	20	0.15	3	0	1	0	0	0	2
...............................................................................................................................TGCTGCCATCTAAT------------CGT-----ATT.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	20	20	0.15	3	3	0	0	0	0	0
.........................................................................................................................TTTTACTGCTGCCATCTAAT------------..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	20	20	0.15	3	3	0	0	0	0	0
............................................................................................................................TACTGCTGCCATCTAAT------------CGT-----ATTCC.....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	25	20	0.10	2	1	1	0	0	0	0
.......................................................................................................................................TCTAAT------------CGT-----ATTCCTAAA--TATG---------TATA..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................	26	20	0.10	2	2	0	0	0	0	0
........................................................................................................................................CTAAT------------CGT-----ATTCCTAAA--TATG---------TATA..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................	25	20	0.10	2	2	0	0	0	0	0
..............................................................................................................................CTGCTGCCATCTAAT------------CGT-----ATTC......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	22	20	0.10	2	2	0	0	0	0	0
...............................................................................................................................TGCTGCCATCTAAT------------CGT-----ATTCT.....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	22	20	0.10	2	1	0	0	1	0	0
..............................................................................................................................CTGCTGCCATCTAAT------------CGT-----ATT.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	21	20	0.10	2	2	0	0	0	0	0
......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TTTCTTAGGGTA------------------------------------------------GCATGC...................................	18	20	0.10	2	2	0	0	0	0	0
..............................................................................................................................CTGCTGCCATCTAAT------------CGT-----AT........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	20	20	0.10	2	2	0	0	0	0	0
.........................................................................................................................TTTTACTGCTGCCATCTAAT------------CGT-----ATTCC.....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	28	20	0.05	1	1	0	0	0	0	0
....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ATTTTCTTAGGGTA------------------------------------------------GCATGCCT.................................	22	20	0.05	1	0	0	0	1	0	0
.........................................................................................................TA-TATATGTGAAGCATTTTACTGCTG.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	26	20	0.05	1	1	0	0	0	0	0
......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TTTCTTAGGGTA------------------------------------------------GCATGCCTGT...............................	22	20	0.05	1	0	0	0	0	1	0
.......................................................................................................AATA-TATATGTGAAGCATTTTACTGC.........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	26	20	0.05	1	0	1	0	0	0	0
...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CATTTTCTTAGGGTA------------------------------------------------GCATGCA..................................	22	20	0.05	1	1	0	0	0	0	0
...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GTA------------------------------------------------GCATGCCTGTAAAGTTAC.......................	21	20	0.05	1	0	0	0	0	0	1
...........................................................................................................................TTACTGCTGCCATCTAAT------------CGA-----..........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	21	20	0.05	1	0	0	0	0	0	1
................................................................................................................................GCTGCCATCTAAT------------CGT-----ATTCC.....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	21	20	0.05	1	0	0	0	0	0	1
........................................................................................................................ATTTTACTGCTGCCATCTAAT------------CGT-----A.........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	25	20	0.05	1	1	0	0	0	0	0
..................................................................................................................................TGCCATCTAAT------------CGT-----ATTCCTA...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	21	20	0.05	1	1	0	0	0	0	0
...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GTA------------------------------------------------GCATGCCTGTAAAGTT.........................	19	20	0.05	1	1	0	0	0	0	0
..............................................................................................................................CTGCTGCCATCTAAT------------CGT-----A.........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	19	20	0.05	1	1	0	0	0	0	0
..............................................................................................................TATGTGAAGCATTTTACTGCT........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	21	20	0.05	1	0	0	0	0	1	0
........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TCTTAGGGTA------------------------------------------------GCATGCCTGT...............................	20	20	0.05	1	1	0	0	0	0	0
..............................................................................................................TATGTGAAGCATTTTACTGCTGCCATC..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	27	20	0.05	1	0	1	0	0	0	0
..................................................................................................................TGAAGCATTTTACTGCTGCCA....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	21	20	0.05	1	1	0	0	0	0	0
...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CATTTTCTTAGGGTA------------------------------------------------GCATGT...................................	21	20	0.05	1	0	0	0	0	1	0
.....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TTTTCTTAGGGTA------------------------------------------------GCATGCCTGTAA.............................	25	20	0.05	1	1	0	0	0	0	0
............................................................................................................TATATGTGAAGCATTTTACTG..........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	21	20	0.05	1	0	0	0	0	1	0
.....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TGCCTGTAAAGTTACAAAA---------..........	19	20	0.05	1	1	0	0	0	0	0
.............................................................................................................ATATGTGAAGCATTTTACTGCTGCCA....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	26	20	0.05	1	1	0	0	0	0	0
...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CATGCCTGTAAAGTTACAAA....................	20	20	0.05	1	0	0	0	0	0	1
...........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TAAAGTTACAAAA---------ATCAAAAA..	21	20	0.05	1	0	0	0	0	0	1
..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CCATTTTCTTAGGGTA------------------------------------------------GCATT....................................	21	20	0.05	1	0	0	0	0	1	0
.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CCCATTTTCTTAGGGTA------------------------------------------------GCATG....................................	22	20	0.05	1	0	0	0	0	0	1
.......................................................................................................................................TCTAAT------------CGT-----ATTCCTAAA--TATG---------TA................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	24	20	0.05	1	1	0	0	0	0	0
................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TCCCATTTTCTTAGGGTA------------------------------------------------GCATGCCTG................................	27	20	0.05	1	0	1	0	0	0	0
..............................................................................................................TATGTGAAGCATTTTACTGCTGCCA....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	25	20	0.05	1	1	0	0	0	0	0
............................................................................................................TATATGTGAAGCATTTTACTGCTGCC.....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	26	20	0.05	1	0	1	0	0	0	0
....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ATTTTCTTAGGGTA------------------------------------------------GCATGC...................................	20	20	0.05	1	0	0	0	0	1	0
.............................................................................................................................ACTGCTGCCATCTAAT------------CGT-----AT........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	21	20	0.05	1	1	0	0	0	0	0
............................................................................................................................TACTGCTGCCATCTAAT------------CGT-----A.........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	21	20	0.05	1	0	0	0	0	0	1
...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CATTTTCTTAGGGTA------------------------------------------------GCATGCCTGTA..............................	26	20	0.05	1	1	0	0	0	0	0
...............................................................................................................ATGTGAAGCATTTTACTGCTGCC.....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	23	20	0.05	1	0	1	0	0	0	0
................................................................................................................................GCTGCCATCTAAT------------CGT-----ATTC......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	20	20	0.05	1	1	0	0	0	0	0
................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TA------------------------------------------------GCATGCCTGTAAAGTTACAA.....................	22	20	0.05	1	1	0	0	0	0	0
.........................................................................................................TA-TATATGTGAAGCATTTTACTGCTGCC.....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	28	20	0.05	1	0	1	0	0	0	0
..........................................................................................................................TTTACTGCTGCCATCTAAT------------CGT-----..........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	22	20	0.05	1	0	0	0	0	0	1
...........................................................................................................................TTACTGCTGCCATCTAAT------------CGG-----..........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	21	20	0.05	1	0	0	0	0	1	0
...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CATTTTCTTAGGGTA------------------------------------------------GCATGCCTG................................	24	20	0.05	1	0	0	0	0	1	0
..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GGTA------------------------------------------------GCATGCCTGTAAAGTTA........................	21	20	0.05	1	0	0	0	0	0	1
........................................................................................................................ATTTTACTGCTGCCATCTAAT------------..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	21	20	0.05	1	1	0	0	0	0	0
..........................................................................................................................TTTACTGCTGCCATCTAAT------------CGT-----ATTC......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	26	20	0.05	1	1	0	0	0	0	0

droBip1

TGTATATTTT-------------------------------------------------------------------------------------TTCA--CAAATT-GTTA------------------AATCCAGTTCA--------------------TGTGATTGTCACAAT---------AATAAACTTTTAC----------------------------------------------ACGTTGAG----------------------------------------------------------------------------------------------------------------AACG---AAATACAAATAAAAAAATTGAAAATTATTCAAA------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------A	Size	Hit Count	Total Norm	Total
No Reads

dp5

TATAGAACTTAAAAAATCAAATAATGACTACCAATCTTTGG---------GTGGAGCAGG-----TA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
........................TGACTACCAATCTTTGG---------GTGGAG...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	23	1	2.00	2	0	0	0	0	0	0	0	2	0	0

droPer2

TGCAGAACTTGAAGAATCAAATTATGACCACCAATGTGTGG---------GTGGAGCAGG-----TAAGCTT---------------------------------------------------------------------------------------------------------------------------AACTAT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AAAT	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
No Reads

droWil2

TTCAGAATTTAAAAAATCAAATTATGACTACCAATGTATGG---------GTGGAGCAGG-----TAAGTGTT---------------------------------------------------------------------------------------------------------------------------ATCAATCAATACACAAACACAAATAAAATAATAATAATAAATTTAAG---------AAA---------------------------------TTTAAAT----------------------------------------------------------------GAATTTGCTCA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ACTGTAC	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
No Reads

droVir3

TGCAGAATCTAAAAAATCAAATTATGACTACCAACGTTTGG---------GTGGAGCAGG-----TGAGTCA---------------------------------------------------------------------------------------------------------------------------AACTT-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TCCCATTTT-------------------TGTA--------------------------------------------AAAT	Size	Hit Count	Total Norm	Total	M027 Male-body	M028 Head	M047 Female-body	M061 Embryo	SRR060654 Ovary	SRR060655 Testis	SRR060656 Ovary	SRR060657 Testis	SRR060658 Ovary	SRR060659 Testis	SRR060660 Ovary	SRR060661 Embryo	SRR060662 Embryo	SRR060663 Embryo	SRR060664 Male-body	SRR060665 Female-body	SRR060666 Male-body	SRR060667 Female-body	SRR060668 Male-body	SRR060669 Female-body	SRR060670 Testis	SRR060671 Male-body	SRR060672 Female-body	SRR060673 Ovary	SRR060674 Ovary	SRR060675 Ovary	SRR060676 Ovary	SRR060677 Ovary	SRR060678 Testis	SRR060679 Testis	SRR060680 Testis	SRR060681 Testis	SRR060682 Embryo	SRR060683 Testis	SRR060684 Embryo	SRR060685 Embryo	SRR060686 Embryo	SRR060687 Embryo	SRR060688 Ovary	SRR060689 Testis	SRR1106712 embryo_0-2h	SRR1106713 embryo_0-2h	SRR1106714 embryo_2-4h	SRR1106715 embryo_4-6h	SRR1106716 embryo_4-6h	SRR1106717 embryo_6-8h	SRR1106718 embryo_6-8h	SRR1106719 embryo_8-10h	SRR1106720 embryo_8-10h	SRR1106721 embryo_10-12h	SRR1106722 embryo_10-12h	SRR1106723 embryo_12-14h	SRR1106724 embryo_12-14h	SRR1106725 embryo_14-16h	SRR1106726 embryo_14-16h	SRR1106727 larvae	SRR1106728 larvae	SRR1106729 mixed whole adult body	SRR1106730 embryo_16-30h	V047 Embryo	V053 Head	V116 Male-body	GSM1528803 follicle cells
............................TACCAACGTTTGG---------GTGGAG...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0

droMoj3

CACGCTGCCACAAAAAACATATA------------------------------------------------------------------------------------------------------------------------------------------------------------------CATAAG----------------------------------------------------TGCGATGGG----------------------------------------------------------------------------------------------------------------TGCGTA--TACACAGAAAAACAAATTGCCTTTTGTTGCAAAATGTG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GCAAGACGGA----------------------GGA-GCATG	Size	Hit Count	Total Norm	Total	M046 Female-body	V041 Embryo	V049 Head	V056 Head	M060 Embryo	V110 Male-body
.................................................................................................................................................................................................................................................................................................................................................................................................................CAAAATGTG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GCAAGACGGA----------------------GGA-GC...	24	1	20.00	20	13	0	0	0	6	1
.................................................................................................................................................................................................................................................................................................................................................................................................................CAAAATGTG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GCAAGACGGA----------------------GG.......	21	1	13.00	13	10	0	0	0	3	0
.................................................................................................................................................................................................................................................................................................................................................................................................................CAAAATGTG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GCAAGACGGA----------------------GGA-G....	23	1	5.00	5	4	0	0	0	1	0
.................................................................................................................................................................................................................................................................................................................................................................................................................CAAAATGTG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GCAAGACGGA----------------------G........	20	1	3.00	3	2	0	0	0	0	1
.................................................................................................................................................................................................................................................................................................................................................................................................................CAAAATGTG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GCAAGACGGA----------------------GGA-GCA..	25	1	3.00	3	3	0	0	0	0	0
.................................................................................................................................................................................................................................................................................................................................................................................................................CAAAATGTG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GCAAGACGGA----------------------GGA-.....	22	1	2.00	2	2	0	0	0	0	0
..................................................................................................................................................................................................................................................................................................................................................................................................................AAAATGTG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GCAAGACGGA----------------------GGA-GC...	23	1	2.00	2	2	0	0	0	0	0
.................................................................................................................................................................................................................................................................................................................................................................................................................CAAAATGTG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GCAAGACGGA----------------------.........	19	1	1.00	1	1	0	0	0	0	0
..............AAACATATA------------------------------------------------------------------------------------------------------------------------------------------------------------------CATAAG----------------------------------------------------TGCGATG.................................................................................................................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	1	0	0	0	0	0
..................................................................................................................................................................................................................................................................................................................................................................................................................AAAATGTG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GCAAGACGGA----------------------G........	19	1	1.00	1	1	0	0	0	0	0
................................................................................................................................................................................................................................................................................................................................................................................TA--TACACAGAAAAACAAATTGCCTT................................................................................................................................................................................................................................................................	25	1	1.00	1	1	0	0	0	0	0
.................................................................................................................................................................................................................................................................................................................................................................................................................CAAAATGTG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GCAAGACGGA----------------------GGA-A....	23	1	1.00	1	0	0	0	0	0	1
................................................................................................................................................................................................................................................................................................................................................................................................................GCAAAATGTG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GCAAGACGGA----------------------GGA-GC...	25	1	1.00	1	1	0	0	0	0	0
...............................................................................................................................................................................................................................................................................................................................................................................GTA--TACACAGAAAAACAAATTGCC..................................................................................................................................................................................................................................................................	24	1	1.00	1	1	0	0	0	0	0

droGri2

ACCAATTTT-----------------------------TCGCAAAAGGGAAACGAGGAAG-----CAAATAATTACACATTTTTGTATGT-----TTGA--CAAAT---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTAAAT----------------------------------------------------------------------------------------AAATATTGCTTTTCTATGAAAA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AGA-------TGTGTA--------------------------------AATATTT	Size	Hit Count	Total Norm	Total	M041 Female-body	V109 Male-body
No Reads

dsi_29545

3l:22720219-22720368 +

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-7.4	-7.3