dsi_25671

GGCCTTTGTGACGTATGCAACTCGCTTACACGCTTAGATCCTTATACGACTTAGAGTCATGTGGAAGAGCAGTTTAGCCTTCCACAATAGCTGCAAGAAAATCTTGCATTTAGCAGCTGCAGGTCCGATAAGAACAAAAGCGATGTAAATATAAGCCAGAATAAGTAACTATAAAATACGTTTATCGTAACGCTTATTAGGTATTTTAGTTTGAGGCAGACACAAGAAAATAATAAATTATCCGTAAAAG *******************************************************************************************************************....(((...(((...(((.......)))...)))..)))............*******************************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	O001 Testis	SRR553486 Makindu_3 day-old ovaries	SRR553488 RT_0-2 hours eggs	M053 female body	SRR553487 NRT_0-2 hours eggs	SRR618934 dsim w501 ovaries	SRR553485 Chicharo_3 day-old ovaries	GSM343915 embryo	M025 embryo
..............ATGCAACGCGCTCACACGC.........................................................................................................................................................................................................................	19	2	2	0.50	1	1	0	0	0	0	0	0	0	0
...................................................................................................................................................AATATAAGCCAGAATAAGTAAC.................................................................................	22	0	10	0.30	3	3	0	0	0	0	0	0	0	0
.....................................................................................................................TGCAGGTCCGATAAGAACAAAAGCGA...........................................................................................................	26	0	5	0.20	1	0	0	1	0	0	0	0	0	0
................................................................................................................................TAAGAACAAAAGCGATGTAAATAT..................................................................................................	24	0	5	0.20	1	0	1	0	0	0	0	0	0	0
............GTCTGCAACTCGCCTACATG..........................................................................................................................................................................................................................	20	3	5	0.20	1	0	0	0	1	0	0	0	0	0
.................................................................................................................................................................................................TTATTAGGTATTTTAGTTTGAGACAGA..............................	27	1	5	0.20	1	0	0	0	0	1	0	0	0	0
........................................................................................................TGCATTTAGCAGCTGCAGGTCCGATAAGA.....................................................................................................................	29	0	5	0.20	1	0	1	0	0	0	0	0	0	0
...........................................................................................................................................................................................................TTTTAGTTTGAGGCAGACACAAGAAAA....................	27	0	5	0.20	1	0	1	0	0	0	0	0	0	0
.................................................................................................................................................................TAAGTAACTATAAAATACGTTTATCGT..............................................................	27	0	5	0.20	1	0	0	1	0	0	0	0	0	0
.......................................................................................................................CAGGTCCGAAAAGAAGA..................................................................................................................	17	2	20	0.10	2	0	0	0	0	0	0	2	0	0
...............TGCAACTCGCCTACATGA.........................................................................................................................................................................................................................	18	3	20	0.10	2	0	0	0	2	0	0	0	0	0
.GCCTTGGTGACGTTTGC........................................................................................................................................................................................................................................	17	2	13	0.08	1	0	0	0	0	0	1	0	0	0
..................................................................................................................................................................................................TATTTGGTATTCTAGTTGGAG...................................	21	3	15	0.07	1	0	0	0	0	0	0	0	1	0
...................................................................................................................................................AATATAAGACAGAATA.......................................................................................	16	1	16	0.06	1	0	0	0	0	0	1	0	0	0
...................................................................................................................................TAACAAAACCGATGTAAA.....................................................................................................	18	2	18	0.06	1	0	0	1	0	0	0	0	0	0
.........................................................................................................................................................................................CGTAACGATTAATAGGGA...............................................	18	3	20	0.05	1	0	0	0	0	0	0	0	0	1

CCGGAAACACTGCATACGTTGAGCGAATGTGCGAATCTAGGAATATGCTGAATCTCAGTACACCTTCTCGTCAAATCGGAAGGTGTTATCGACGTTCTTTTAGAACGTAAATCGTCGACGTCCAGGCTATTCTTGTTTTCGCTACATTTATATTCGGTCTTATTCATTGATATTTTATGCAAATAGCATTGCGAATAATCCATAAAATCAAACTCCGTCTGTGTTCTTTTATTATTTAATAGGCATTTTC *******************************************************************************....(((...(((...(((.......)))...)))..)))............*******************************************************************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	SRR553486 Makindu_3 day-old ovaries	SRR553487 NRT_0-2 hours eggs	SRR553488 RT_0-2 hours eggs	SRR553485 Chicharo_3 day-old ovaries	M024 male body	SRR618934 dsim w501 ovaries	M053 female body
...............AGGATGAGCGAATGTG...........................................................................................................................................................................................................................	16	2	20	0.90	18	18	0	0	0	0	0	0
...........................................................................................................................................................................................AGTGCGAATGATCCATAA.............................................	18	2	6	0.50	3	1	1	1	0	0	0	0
...........................................................................................................................................................................................AGTGCGAATGATCCATAAG............................................	19	3	9	0.22	2	0	2	0	0	0	0	0
..............................................................................................................ACCGTCGACGTCCAGGCTATGT......................................................................................................................	22	3	8	0.13	1	0	0	0	0	1	0	0
.................................................................................................................................................................................................................AATCTCCGTCAGTGATCTTTTAT..................	23	3	8	0.13	1	0	1	0	0	0	0	0
...........................................................................................................................................................................................AGTGCGAATGATCCATA..............................................	17	2	10	0.10	1	0	1	0	0	0	0	0
...............................................................................................ACTTTTAGAACGTAAATCGTCGACGT.................................................................................................................................	26	1	11	0.09	1	0	0	0	1	0	0	0
...................................................................TCGTCAAATCGGAAGGTGT....................................................................................................................................................................	19	0	11	0.09	1	0	0	0	0	0	1	0
.........................................................................................................................................................................................TGATTGCGAATGATCCATAA.............................................	20	3	13	0.08	1	0	0	1	0	0	0	0
.............................................................................................................................................................................................TGCGAATGATCCATAA.............................................	16	1	17	0.06	1	0	1	0	0	0	0	0
.....................................................................................................................................................................................................................................TATTATTTATTAGGCATA...	18	2	20	0.05	1	0	0	0	0	0	0	1
...........................................................................................................................................................................................ATTGCGAATGATCCTT...............................................	16	2	20	0.05	1	1	0	0	0	0	0	0
..............................................................................................................................................................................................GCGAATGATCCATAA.............................................	15	1	20	0.05	1	0	0	0	1	0	0	0
...........................................................................................................................................................................................AGTGCGAATGATCCA................................................	15	2	20	0.05	1	0	0	1	0	0	0	0

Species	Coordinate	ID	Alignment
droSim2	2l:23250514-23250763 -	dsi_25671	GGCCTTTGTGACGTATGCAACTCGCTTACACGCTTAGATCCTTATACGACTTAGAGTCATGTGGAAGAGCAGTTTAGCCTTCCACAATAGCTGCAAGAAAATCTTGCATTTAGCAGCTGCAGGTCCGATAAGAACAAAAGCGATGTAAATATAAGCCAGAATAAGTAACTATAAAATACGTTTATCGTAACGCTTATTAGGTATTT-------TAGTTTGAGGCAGACACAA----GAAAATAATAAATTATCCGTAAAAG
droSec2	scaffold_76:62326-62501 +		A-----------------------------------------------------------------GAGCAGTTTAGCCTTCCACAATAGCTGCAAGAAAATCTTGCATTTAGCAGCTGCAGGTCCGATGAGAACAAAAAGGATGTTAATATAAGCCAGAATAAGTAACTATAAGATAG---------AACGCTTATTAGGTATTT-------TAGTTTGAGACAGACACAA----GAAGATAATAAATTATCCGTAAAAG
dm3	chr3L:24143449-24143576 -		A----------------------------------------------------------------------GTTCAGCATTCCACAATTGTTGCAAGAAGATCTGGCATATAGCAACTGCAGGTCCAATGAGACCAAGGTGGTCGTAGATATGAGCAATAGTCG------ATAAGATAG---------AACGCTTACTAGGTTTTT-------TAGTTTG-----------------------------------------
droEre2	scaffold_4784:25547049-25547219 -		---------------------------------------------------------------------CAGTGTAGCCTTCTACAATTGCTGCAAGAAAATCTTGCATTTAGCAACTGCAGGTCCGATAAGTCCCAGAAAGTCGTAAGTATAAGCAATATTCG------ATAAGATAG---------AGCGCTTACTAGGTTTTTT------TAGTTTAAGGCAGAGACAAAAACGAAAATAATAAATTATCCGTAGATG
droYak3	v2_chr2L_random_010:166965-167131 -		---------------------------------------------------------------------CAGTTCAGCCTTCCACAATTGCTGCAAGAAAATCTTGCAATTAGCAACTGCAGGTACGAAAAGACCAAAAGGGTCGTAAATATGAGCAATATTCG------ATAAGATAG---------AACCCTTTCTACGTTTTA--------AATTTGAGGCACACATAAGGACG--AATAATAAATTCTCCGTAGATG
droBia1	scf7180000299726:1038-1092 -		T----------------------------------------------------------------------ATT----------------------------------------------------------------------------------------------------------------------------AAGAATT--------TACTTTAAGATAAAAACAA----GAAAACAACAATATATTGACAAAAA
droTak1	scf7180000415315:8459-8615 -		---------------------------------------------------------------------CAGCTTTGCCTTCCATAGATTCTGCAAAAAAATCTTGCACTTTGCCATTACTGGTCCGATAAGGCCAAGGGGGTTGTATAAATGGGCCACAGTTG------ATAGGATGG---------AACGTTTACTGGGTTTTG-------TACTTTGGGGTAGCGATAACAATGAAAACAAGAAA-------------
droEle1	scf7180000487787:2001-2165 +		------------------------------------------------------------------------TTTAGCCTTCCATAAATTGAGCAA-AAAATCTTGCAC-TTGCAATTACCGGTCCGATAAAGCCAAGAGGGTCGTAGAAATGGGCAACATTTG------ATAGGATGG---------AACGCTTACTGGGTTTTG-------CACTTTGTGGTAGCGATAACAATGAAAATAATAAATTCTCAATAGAGG
droRho1	scf7180000779853:2344-2411 +		---------------------------------------------------------------------CAACTTCGCTCTACAGACTTGTTGCAGGAAAATCTTGGCCTTGGCAACCACAGGACCAATGAGACCAA----------------------------------------------------------------------------------------------------------------------------
droAna3	scaffold_13250:2189831-2189888 +		----------------------------------------------------------------------------------CACAGCTTCTGCAAGAATATTTTTGATTTGGTAGCGACTGGACCAACAAGGCCCAAAG-------------------------------------------------------------------------------------------------------------------------
dp5	Unknown_group_93:5478-5646 +		A----------------------------------------------------------------------GCTTTTCTTTCCACAGCTGCTGTAGAAATATTTTTGCTTTAGTAATTACTGACCCTATAAGTCCGAGTGGATCATAAAATCGTGCAATAGTTG------ACAATACAA---------TTCGCTTAGTTGGTTTCGAAGGGACT------TGATTGGGAC-AGAATGAAAATAATAGCTCATCCGAAGTGG
droPer2	scaffold_1701:1465-1633 -		A----------------------------------------------------------------------GCTTTTCTTTCCACAGCTGCTGTAGAAATATTTTTGCTTTAGTAATAACTGGCCCTATAAGTCCGAGTGGATCATAAAATCGTGCAATAGTTG------ACAATACAA---------CTCGCTTAGTTGGTTTCGAAGGGACT------TGATTGGGAC-AGAATGAAAATAATAGCTTATCCGAAGTGG
droVir3	scaffold_12891:139686-139784 -		A----------------------------------------------------------------------GCTTCTCCTTCCAGATCTGCTGCATAAAGATTTTAGCCTTGGCAATTACCAGCCCTATGAGTCCAAGCGGGTCATACAAGCGAGCTATAGTCG------ATAGC--------------------------------------------------------------------------------------
droMoj3	scaffold_6540:772839-773007 -		A----------------------------------------------------------------------GTTTATACCTCCAGAGTTGTTGCAAGAAAATTTTAGCCTTCGTTATTACTGGGCCGACCAGGCCGAGGGGATCATAAAACCGAGCAATAGACG------AGAGAACAG---------AGCGCCTGGAGGGCTTAG-------AGGCTGAATTCAGGGCTAGATATGAGAAAAGAAGTTGATCAGATGCCG
droGri2	scaffold_14940:4150-4315 +		-------------------------------------------------------------------------TTTGCCTTCCAGAGTTGCTGCAGGAATATTTTGCACCTAGCAATTGCAGGTCCAATAAGACCAAGAGGATCATAGATACACGCAATCGTGT------ACAAGATGG---------ATCGCTTGGAGAGTTTTG-------AATTTGGGCGAACAGGTGACAACAAAAACAAAAGATTATCCGAAACTG

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droSim2

GGCCTTTGTGACGTATGCAACTCGCTTACACGCTTAGATCCTTATACGACTTAGAGTCATGTGGAAGAGCAGTTTAGCCTTCCACAATAGCTGCAAGAAAATCTTGCATTTAGCAGCTGCAGGTCCGATAAGAACAAAAGCGATGTAAATATAAGCCAGAATAAGTAACTATAAAATACGTTTATCGTAACGCTTATTAGGTATTT-------TAGTTTGAGGCAGACACAA----GAAAATAATAAATTATCCGTAAAAG	Size	Hit Count	Total Norm	Total	M023 Head	M024 Male-body	M025 Embryo	M053 Female-body	O001 Testis	O002 Head	SRR553485 Ovary	SRR553486 Ovary	SRR553487 Ovary	SRR553488 Ovary	SRR618934 Ovary	V044 Embryo	SRR902008 Ovary	SRR902009 Testis	SRR1275487 Male larvae	SRR1275485 Male prepupae	SRR1275483 Male prepupae
...................................................................................................................................................AATATAAGCCAGAATAAGTAAC............................................................................................	22	10	0.30	3	0	0	0	0	3	0	0	0	0	0	0	0	0	0	0	0	0
.....................................................................................................................TGCAGGTCCGATAAGAACAAAAGCGA......................................................................................................................	26	5	0.20	1	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0
................................................................................................................................TAAGAACAAAAGCGATGTAAATAT.............................................................................................................	24	5	0.20	1	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0
........................................................................................................TGCATTTAGCAGCTGCAGGTCCGATAAGA................................................................................................................................	29	5	0.20	1	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0
...........................................................................................................................................................................................................TTT-------TAGTTTGAGGCAGACACAA----GAAAA....................	27	5	0.20	1	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0
.................................................................................................................................................................TAAGTAACTATAAAATACGTTTATCGT.........................................................................	27	5	0.20	1	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0

droSec2

A-----------------------------------------------------------------GAGCAGTTTAGCCTTCCACAATAGCTGCAAGAAAATCTTGCATTTAGCAGCTGCAGGTCCGATGAGAACAAAAAGGATGTTAATATAAGCCAGAATAAGTAACTATAAGATAG---------AACGCTTATTAGGTATTT-------TAGTTTGAGACAGACACAA----GAAGATAATAAATTATCCGTAAAAG	Size	Hit Count	Total Norm	Total	M054 Female-body	V113 Male-body	V114 Embryo	V115 Head
No Reads

dm3

A----------------------------------------------------------------------GTTCAGCATTCCACAATTGTTGCAAGAAGATCTGGCATATAGCAACTGCAGGTCCAATGAGACCAAGGTGGTCGTAGATATGAGCAATAGTCG------ATAAGATAG---------AACGCTTACTAGGTTTTT-------TAGTTTG-----------------------------------------	Size	Hit Count	Total Norm	Total	GSM154618 Ovary	GSM154620 Ovary	GSM154621 Ovary	GSM154622 Ovary	GSM180328 Head	GSM180329 Mixed-body	GSM180330 Embryo	GSM180331 Embryo	GSM180332 Embryo	GSM180333 Embryo	GSM180334 Larvae	GSM180335 Disc	GSM180336 Pupae	GSM180337 S2	GSM231091 Ovary	GSM239041 Head	GSM239050 Head	GSM239051 S2	GSM239052 S2	GSM246084 Head	GSM266765 S2	GSM280082 Ovary	GSM280083 Ovary	GSM280084 Ovary	GSM280085 Testis	GSM280086 Ovary	GSM280087 S2-NP	GSM280088 S2-NP	GSM312995 Head	GSM313160 Head	GSM313161 Head	GSM313162 Head	GSM313163 Head	GSM313164 Head	GSM313165 Head	GSM343287 Embryo	GSM379050 Ovary	GSM379051 Ovary	GSM379052 Ovary	GSM379053 Ovary	GSM379054 Ovary	GSM379055 Ovary	GSM379056 Ovary	GSM379057 Ovary	GSM379058 Ovary	GSM379059 Ovary	GSM379060 Ovary	GSM379061 Ovary	GSM379062 Ovary	GSM379063 Ovary	GSM379064 Ovary	GSM379065 Ovary	GSM379066 Ovary	GSM379067 Ovary	GSM424739 S2	GSM424740 S2	GSM424741 S2	GSM467729 Female-body	GSM467730 Female-body	GSM467731 Female-body	M002 CS	M003 Male-body	M004 Male-body	M005 ML-DmD32	M006 ML-DmD32	M029 OSC	M030 OSC	M031 OSC	M032 S2-R+	M033 S2-R+	M034 S2-R+	M035 S2-R+	M036 S2-R+	M037 S2-R+	M038 S2-R+	M039 S2-R+	M048 S2-R+	OSS2 OSS	OSS6 OSS	OSS7 OSS	OSS8 OSS	S1 Head	S10 Embryo	S11 Embryo	S12 Embryo	S13 Embryo	S14 Embryo	S15 S2-GMR	S16 Kc	S17 Larvae	S18 Larvae	S19 Pupae	S2 Head	S20 Pupae	S21 Disc	S22 Head	S23 Female-body	S24 Male-body	S25 Larvae	S26 Larvae	S27 Pupae	S28 Pupae	S29 Head	S3 Male-body	S30 S2-GMR	S31 S2	S32 S2	S33 Kc	S34 Kc	S35 Kc	S4 Female-body	S5 Disc	S6 Embryo	S7 Embryo	S8 S2	S9 Embryo	SRR001337 Head	SRR001338 Head	SRR001339 Head	SRR001340 Head	SRR001341 Head	SRR001342 Head	SRR001343 Head	SRR001344 Head	SRR001345 Head	SRR001346 Head	SRR001347 Head	SRR001348 Head	SRR001349 Head	SRR001664 Head	SRR010951 Ovary	SRR010952 Ovary	SRR010953 Ovary	SRR010954 Ovary	SRR010955 Ovary	SRR010956 Ovary	SRR010957 Ovary	SRR010958 Ovary	SRR010959 Ovary	SRR010960 Ovary	SRR014268 Embryo	SRR014269 Embryo	SRR014270 Embryo	SRR014271 Embryo	SRR014272 Embryo	SRR014273 Ovary	SRR014274 Ovary	SRR014275 Ovary	SRR014276 Ovary	SRR014277 Ovary	SRR014278 Ovary	SRR014279 Ovary	SRR014280 Ovary	SRR014281 Ovary	SRR014282 Ovary	SRR023197 S2	SRR023399 S2	SRR023400 S2	SRR023402 S2	SRR023407 S2	SRR029028 S2	SRR029029 S2	SRR029030 S2	SRR029031 S2	SRR029032 S2	SRR029033 S2	SRR029608 Head	SRR029633 Head	SRR030145 gb3	SRR031692 Head	SRR031693 Head	SRR031694 Head	SRR031696 Head	SRR031697 Head	SRR031698 Head	SRR031699 Head	SRR031700 Head	SRR031701 Head	SRR031702 Head	SRR031703 Head	SRR031704 Head	SRR032092 S2	SRR032093 S2	SRR032094 S2	SRR032095 S2-NP	SRR032096 S2-NP	SRR032152 Head	SRR060643 Testis	SRR060644 Ovary	SRR060645 Testis	SRR060646 Ovary	SRR060647 Ovary	SRR060648 Ovary	SRR060649 Ovary	SRR060650 Testis	SRR060651 Ovary	SRR060652 Testis	SRR060653 Ovary	SRR065151 Ovary	SRR065152 Ovary	SRR065153 Ovary	SRR065154 Ovary	SRR065155 Ovary	SRR065800 Ovary	SRR065801 Ovary	SRR065802 Ovary	SRR065803 Ovary	SRR065804 Ovary	SRR065805 Ovary	SRR065806 Ovary	SRR065807 Ovary	SRR097865 S2-NP	SRR097866 S2-NP	SRR097867 S2-NP	SRR298536 Ovary	SRR298537 Ovary	SRR298711 Ovary	SRR298712 Ovary	SRR317110 Ovary	SRR317111 Ovary	SRR317112 Ovary	SRR317113 Ovary	SRR317114 Ovary	SRR317115 Ovary	SRR317116 Ovary	SRR317117 Ovary	SRR341115 S2	SRR341116 S2	SRR341117 S2	SRR341118 S2	SRR351332 Mixed-body	SRR351333 Mixed-body	T004 OSC	T005 CME-L1	V001 Embryo	V002 Embryo	V003 S2	V006 Female-body	V007 Head	V008 S2	V009 CME-L1	V010 ML-DmD20-C5	V011 Sg4	V012 Male-body	V013 Head	V014 DTT	V015 DreRFHV	V018 Kc167	V019 GM2	V020 S2-R+	V021 ML-DmD21	V022 ML-DmD32	V023 Head	V024 Kc167	V025 S2-R+	V026 1182-2H	V027 ML-DmD21	V028 CME-W1-Cl.8+	V029 ML-DmD9	V030 ML-DmD8	V031 GM2	V032 S1	V033 CME-W1-Cl.8+	V034 ML-DmD16-C3	V035 ML-DmD32	V036 ML-DmD20-C5	V037 S2	V038 S2	V063 Ovary	V0632 Ovary	V064 Ovary	V0642 Ovary	V065 Ovary	V0652 Ovary	V066 Ovary	V0662 Ovary	V067 S2-NP	V073 mbn2	V074 S3	V075 ML-DmBG1-C1	V076 ML-DmBG3-C2	V077 Head	V078 Head	V079 Head	V080 Head	V081 Embryo	V082 Embryo	V083 Male-body	V084 Female-body	V085 CME-W2	V086 Female-body	V088 Ovary	V0882 Ovary	V089 Ovary	V0892 Ovary	V090 Male-body	V091 fGS/OSS	V092 Male-body	V093 Male-body	V094 S2-R+	V095 S2-R+	V096 Ovary	V097 Ovary	V098 Ovary	V125 ML-DmD9	V126 CME-L1	V127 G2	V128 S3	V129 ML-DmBG1-C1	V130 ML-DmBG3-C2	V131 ML-DmD16-C3	V132 ML-DmD32	V133 Sg4	V134 ML-DmD8	V135 CME-W2	V136 Male-body	V137 Head	V138 Male-body	V139 Female-body	V140 Female-body	V141 Female-body	V142 Female-body	V144 OSC	V145 S2	V146 S1	V147 1182-2H	V148 mbn2	GSM1528798 follicle cells
..............................................................................................................................................TCGTAGATATGAGCAATAGTCG------ATAAGA.....................................................................................	28	1	7.00	7	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...........................................................................................................................................................................TAAGATAG---------AACGCTTACTAGGTTTT........................................................	25	1	5.00	5	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
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...............................................................................TTCCACAATTGTTGCAAGAAGATC..............................................................................................................................................................	24	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
........................................................................................................................................................GAGCAATAGTCG------ATAAGATAG---------AACGCT...................................................................	27	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...................................................................................................................................GACCAAGGTGGTCGTACCC...............................................................................................................	19	8	0.13	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

droEre2

---------------------------------------------------------------------CAGTGTAGCCTTCTACAATTGCTGCAAGAAAATCTTGCATTTAGCAACTGCAGGTCCGATAAGTCCCAGAAAGTCGTAAGTATAAGCAATATTCG------ATAAGATAG---------AGCGCTTACTAGGTTTTTT------TAGTTTAAGGCAGAGACAAAAACGAAAATAATAAATTATCCGTAGATG	Size	Hit Count	Total Norm	Total	M055 Female-body	M057 Embryo	V060 Head	V040 Embryo	V107 Male-body	V108 Head	GSM1528801 follicle cells
No Reads

droYak3

---------------------------------------------------------------------CAGTTCAGCCTTCCACAATTGCTGCAAGAAAATCTTGCAATTAGCAACTGCAGGTACGAAAAGACCAAAAGGGTCGTAAATATGAGCAATATTCG------ATAAGATAG---------AACCCTTTCTACGTTTTA--------AATTTGAGGCACACATAAGGACG--AATAATAAATTCTCCGTAGATG

Size

Hit Count

Total Norm

Total

M026
Head

M043
Female-body

M056
Embryo

V046
Embryo

V052
Head

V058
Head

V120
Male-body

SRR1275488
Male larvae

SRR1275486
Male prepupae

SRR1275484
Male prepupae

GSM1528802
follicle cells

No Reads

droBia1

T----------------------------------------------------------------------ATT----------------------------------------------------------------------------------------------------------------------------AAGAATT--------TACTTTAAGATAAAAACAA----GAAAACAACAATATATTGACAAAAA	Size	Hit Count	Total Norm	Total
No Reads

droTak1

---------------------------------------------------------------------CAGCTTTGCCTTCCATAGATTCTGCAAAAAAATCTTGCACTTTGCCATTACTGGTCCGATAAGGCCAAGGGGGTTGTATAAATGGGCCACAGTTG------ATAGGATGG---------AACGTTTACTGGGTTTTG-------TACTTTGGGGTAGCGATAACAATGAAAACAAGAAA-------------	Size	Hit Count	Total Norm	Total
No Reads

droEle1

------------------------------------------------------------------------TTTAGCCTTCCATAAATTGAGCAA-AAAATCTTGCAC-TTGCAATTACCGGTCCGATAAAGCCAAGAGGGTCGTAGAAATGGGCAACATTTG------ATAGGATGG---------AACGCTTACTGGGTTTTG-------CACTTTGTGGTAGCGATAACAATGAAAATAATAAATTCTCAATAGAGG	Size	Hit Count	Total Norm	Total
No Reads

droRho1

---------------------------------------------------------------------CAACTTCGCTCTACAGACTTGTTGCAGGAAAATCTTGGCCTTGGCAACCACAGGACCAATGAGACCAA----------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droAna3

----------------------------------------------------------------------------------CACAGCTTCTGCAAGAATATTTTTGATTTGGTAGCGACTGGACCAACAAGGCCCAAAG-------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M044 Female-body	M058 Embryo	V039 Embryo	V055 Head	V105 Male-body	V106 Head
No Reads

dp5

A----------------------------------------------------------------------GCTTTTCTTTCCACAGCTGCTGTAGAAATATTTTTGCTTTAGTAATTACTGACCCTATAAGTCCGAGTGGATCATAAAATCGTGCAATAGTTG------ACAATACAA---------TTCGCTTAGTTGGTTTCGAAGGGACT------TGATTGGGAC-AGAATGAAAATAATAGCTCATCCGAAGTGG	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
...............................................................................................................................................................................................................AGGGACT------TGATTGGGAC-AGAATG........................	23	9	20.00	180	0	102	0	0	0	0	2	47	29	0
...............................................................................................................................................................................................................AGGGACT------TGATTGGGAC-AGAAT.........................	22	9	16.22	146	1	77	1	0	0	0	8	52	7	0
...............................................................................................................................................................................................................AGGGACT------TGATTGGGAC-AGAATGA.......................	24	9	15.56	140	0	53	0	0	0	0	2	51	34	0
...............................................................................................................................................................................................................AGGGACT------TGATTGGGAC-AGAATGAA......................	25	9	6.22	56	0	19	0	0	0	0	1	15	21	0
...........................................................................................TGTAGAAATATTTTTGCTTTAGTAA.................................................................................................................................................	25	1	6.00	6	0	6	0	0	0	0	0	0	0	0
...........................................................................................TGTAGAAATATTTTTGCTTTAGTA..................................................................................................................................................	24	1	6.00	6	0	2	0	0	0	0	0	4	0	0
...............................................................................................................................................................................................................AGGGACT------TGATTGGGAC-AGAA..........................	21	9	5.33	48	0	23	0	0	1	0	1	17	6	0
...............................................................................................................................................................................................................AGGGACT------TGATTGGGAC-AGAATGAAAA....................	27	9	3.11	28	0	19	0	0	0	0	4	2	3	0
...............................................................................................................................................................................................................AGGGACT------TGATTGGGAC-AGA...........................	20	9	3.11	28	0	19	0	0	0	0	0	6	3	0
...............................................................................................................................................................................................................AGGGACT------TGATTGGGAC-AGAAA.........................	22	9	3.00	27	0	13	0	0	0	0	0	13	1	0
...........................................................................................TGTAGAAATATTTTTGCTTTAGT...................................................................................................................................................	23	1	3.00	3	0	0	0	0	0	0	0	3	0	0
...............................................................................................................................................................................................................AGGGACT------TGATTGGGAC-AGAATGAAA.....................	26	9	2.89	26	0	11	0	0	0	0	2	7	6	0
..........................................................................................................................................TGGATCATAAAATCGTGCAATAGTTG------...........................................................................................	26	6	2.00	12	0	12	0	0	0	0	0	0	0	0
..........................................................................................................................................TGGATCATAAAATCGTGCAATAGT...................................................................................................	24	6	1.83	11	0	9	0	0	0	0	0	1	1	0
...............................................................................................................................................................................................................AGGGACT------TGATTGGGAC-AGAATA........................	23	9	1.78	16	0	8	0	0	0	0	0	8	0	0
...............................................................................................................................................................................................................AGGGACT------TGATTGGGAC-AG............................	19	9	1.67	15	0	12	0	0	0	0	0	2	1	0
..........................................................................................................................................TGGATCATAAAATCGTGCAATAGTTG------A..........................................................................................	27	6	1.50	9	0	5	0	0	0	1	0	0	3	0
..........................................................................................................................................TGGATCATAAAATCGTGCAATAG....................................................................................................	23	6	1.50	9	0	6	0	0	0	0	0	3	0	0
...............................................................................................................................................................................................................AGGGACT------TGATTGGGAC-AGAAAA........................	23	9	1.33	12	0	12	0	0	0	0	0	0	0	0
............................................................................................................................................................................................................................TGATTGGGAC-AGAATGAAAATAATAGC.............	27	6	1.33	8	0	0	0	0	0	0	0	3	5	0
................................................................................................................................................................................................................GGGACT------TGATTGGGAC-AGAATG........................	22	9	1.22	11	0	11	0	0	0	0	0	0	0	0
.................................................................................................................................................................................................................GGACT------TGATTGGGAC-AGAATGAAAA....................	25	9	1.22	11	0	11	0	0	0	0	0	0	0	0
.......................................................................................CTGCTGTAGAAATATTTTTGCTTTAG....................................................................................................................................................	26	1	1.00	1	0	1	0	0	0	0	0	0	0	0
..............................................................................................................................................................TAGTTG------ACAATACAA---------TTCGCTTAGT...............................................................	25	1	1.00	1	0	1	0	0	0	0	0	0	0	0
............................................................................................GTAGAAATATTTTTGCTTTAGT...................................................................................................................................................	22	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.................................................................................................................................................................................................................GGACT------TGATTGGGAC-AGAATGAAA.....................	24	9	0.89	8	0	8	0	0	0	0	0	0	0	0
...............................................................................................................................................................................................................AGGGACT------TGATTGGGAC-AGAATC........................	23	9	0.89	8	0	0	0	0	0	0	0	0	8	0
.................................................................................................................................................................................................................GGACT------TGATTGGGAC-AGAATGAA......................	23	9	0.67	6	0	5	0	0	0	0	0	1	0	0
................................................................................................................................................................................................................GGGACT------TGATTGGGAC-AGAATGAAA.....................	25	9	0.67	6	0	4	0	0	0	0	0	2	0	0
.........................................................................................................................................GTGGATCATAAAATCGTGCAATAGTTG------...........................................................................................	27	6	0.67	4	0	4	0	0	0	0	0	0	0	0
..........................................................................TTTCTTTCCACAGCTGCTGTAGA....................................................................................................................................................................	23	6	0.67	4	0	4	0	0	0	0	0	0	0	0
......................................................................................................................................................................................................TGGTTTCGAAGGGACT------TGATTGG..................................	23	8	0.63	5	0	5	0	0	0	0	0	0	0	0
...........................................................................................................................................................................................................................................TGAAAATAATAGCTCATCCGAAG...	23	5	0.60	3	0	3	0	0	0	0	0	0	0	0
................................................................................................................................................................................................................................TGGGAC-AGAATGAAAATAATAGCTCATC........	28	6	0.50	3	0	2	0	0	0	0	0	0	1	0
.......................................................................................................................................GAGTGGATCATAAAATCGTGCAATAG....................................................................................................	26	6	0.50	3	0	0	0	0	0	0	0	2	1	0
................................................................................................................................TAAGTCCGAGTGGATCATAAAATCG............................................................................................................	25	6	0.50	3	0	3	0	0	0	0	0	0	0	0
................................................................................................................................................................................................................................TGGGAC-AGAATGAAAATAATAGC.............	23	6	0.50	3	0	2	0	0	0	0	0	1	0	0
..........................................................................................................................................TGGATCATAAAATCGTGCA........................................................................................................	19	6	0.50	3	0	3	0	0	0	0	0	0	0	0
..........................................................................TTTCTTTCCACAGCTGCTGTAGAAA..................................................................................................................................................................	25	6	0.50	3	0	2	0	0	0	0	0	0	1	0
................................................................................................................................................................................................................GGGACT------TGATTGGGAC-AGAAT.........................	21	9	0.44	4	0	4	0	0	0	0	0	0	0	0
................................................................................................................................................................................................................GGGACT------TGATTGGGAC-AGAATGA.......................	23	9	0.44	4	0	3	0	0	0	0	0	0	1	0
..................................................................................................................................................................................................................GACT------TGATTGGGAC-AGAATGAAAA....................	24	9	0.44	4	0	4	0	0	0	0	0	0	0	0
............................................................................................................................................GATCATAAAATCGTGCAATAGTTG------ACAA.......................................................................................	28	6	0.33	2	0	0	0	0	0	0	0	0	2	0
..........................................................................................................................................TGGATCATAAAATCGTGCAATAGTT..................................................................................................	25	6	0.33	2	0	2	0	0	0	0	0	0	0	0
...........................................................................TTCTTTCCACAGCTGCTGTAGAAA..................................................................................................................................................................	24	6	0.33	2	0	2	0	0	0	0	0	0	0	0
............................................................................................................................................GATCATAAAATCGTGCAATAGTTG------...........................................................................................	24	6	0.33	2	0	2	0	0	0	0	0	0	0	0
.................................................................................................................................................................................................................GGACT------TGATTGGGAC-AGAATGA.......................	22	9	0.33	3	0	3	0	0	0	0	0	0	0	0
..............................................................................TTTCCACAGCTGCTGTAGA....................................................................................................................................................................	19	6	0.33	2	0	0	0	0	0	0	0	2	0	0
................................................................................................................................................................................................................GGGACT------TGATTGGGAC-AGAATGAAAA....................	26	9	0.33	3	0	3	0	0	0	0	0	0	0	0
.......................................................................................................................................GAGTGGATCATAAAATCGTGCAA.......................................................................................................	23	6	0.33	2	0	2	0	0	0	0	0	0	0	0
.........................................................................................................................................GTGGATCATAAAATCGTGCAATAGT...................................................................................................	25	6	0.33	2	0	1	0	0	1	0	0	0	0	0
................................................................................................................................................................................................................GGGACT------TGATTGGGAC-AGAA..........................	20	9	0.33	3	0	1	0	0	0	0	0	2	0	0
...............................................................................................................................................................................................................AGGGACT------TGATTGGGAC-AGAATT........................	23	9	0.33	3	0	2	0	0	0	0	0	1	0	0
.....................................................................................AGCTGCTGTAGAAATATTTTTGC.........................................................................................................................................................	23	7	0.29	2	0	1	0	0	0	0	0	1	0	0
............................................................................................................................................................................................................CGAAGGGACT------TGATTGGGAC-..............................	20	8	0.25	2	0	0	0	0	0	0	0	0	2	0
......................................................................................................................................................................................................TGGTTTCGAAGGGACT------TGATA....................................	21	8	0.25	2	0	2	0	0	0	0	0	0	0	0
...............................................................................................................................................................................................................AGGGACT------TGATTGGGAC-AA............................	19	9	0.22	2	0	2	0	0	0	0	0	0	0	0
...............................................................................................................................................................................................................AGGGACT------TGATTGGGAC-AGAATGAAAAA...................	28	9	0.22	2	0	0	0	0	0	0	0	0	2	0
...............................................................................................................................................................................................................AGGGACT------TGATTGGGAC-AGTT..........................	21	9	0.22	2	0	2	0	0	0	0	0	0	0	0
...............................................................................................................................................................................................................AGGGACT------TGATTGGGAC-..............................	17	9	0.22	2	0	0	0	0	0	0	0	1	1	0
.................................................................................................................................................................................................................GGACT------TGATTGGGAC-AGAATG........................	21	9	0.22	2	0	2	0	0	0	0	0	0	0	0
.................................................................................................................................................................................................................................................TAATAGCTCATCCGAAGTGG	20	5	0.20	1	0	1	0	0	0	0	0	0	0	0
.................................................................................................................................................................................................................................................TAATAGCTCATCCGAAGTG.	19	5	0.20	1	0	1	0	0	0	0	0	0	0	0
...........................................................................................................................................................................................................................................TGAAAATAATAGCTCATCCGAAGTGG	26	5	0.20	1	0	1	0	0	0	0	0	0	0	0
..........................................................................................................................................TGGATCATAAAATCGTGCAATAGTTT------...........................................................................................	26	6	0.17	1	0	0	0	0	0	0	0	1	0	0
................................................................................................................................................................................................................................TGGGAC-AGAATGAAAATAATAGCTC...........	25	6	0.17	1	0	1	0	0	0	0	0	0	0	0
......................................................................................................................................CGAGTGGATCATAAAATCGTG..........................................................................................................	21	6	0.17	1	0	1	0	0	0	0	0	0	0	0
..................................................................................................................................................................................................................GACT------TGATTGGGAC-AGAATGAAAATAA.................	27	6	0.17	1	0	1	0	0	0	0	0	0	0	0
...................................................................................ACAGCTGCTGTAGAAATATT..............................................................................................................................................................	20	6	0.17	1	0	1	0	0	0	0	0	0	0	0
.......................................................................................................................................GAGTGGATCATAAAATCGTGC.........................................................................................................	21	6	0.17	1	0	1	0	0	0	0	0	0	0	0
...................................................................................................................................................................................................................................GAC-AGAATGAAAATAATAGCTCATCC.......	26	6	0.17	1	0	1	0	0	0	0	0	0	0	0
..........................................................................................................................................TGGATCATAAAATCGTGCAAT......................................................................................................	21	6	0.17	1	0	0	0	0	0	0	0	1	0	0
..........................................................................TTTCTTTCCACAGCTGCTGTAGAA...................................................................................................................................................................	24	6	0.17	1	0	1	0	0	0	0	0	0	0	0
.......................................................................................................................................GAGTGGATCATAAAATCGTGCAATAGT...................................................................................................	27	6	0.17	1	0	0	0	0	0	0	1	0	0	0
.................................................................................................................................AAGTCCGAGTGGATCATAAAATCG............................................................................................................	24	6	0.17	1	0	1	0	0	0	0	0	0	0	0
................................................................................................................................................................................................................................TGGGAC-AGAATGAAAATAATAGCTCATCC.......	29	6	0.17	1	0	1	0	0	0	0	0	0	0	0
................................................................................TCCACAGCTGCTGTAGAAATATTT.............................................................................................................................................................	24	6	0.17	1	0	0	0	0	0	0	0	0	1	0
..............................................................................TTTCCACAGCTGCTGTAGAAATATT..............................................................................................................................................................	25	6	0.17	1	0	1	0	0	0	0	0	0	0	0
.............................................................................................................................................................................................................................GATTGGGAC-AGAATGAAAATAATAGC.............	26	6	0.17	1	0	1	0	0	0	0	0	0	0	0
...........................................................................................................................................GGATCATAAAATCGTGCA........................................................................................................	18	6	0.17	1	0	1	0	0	0	0	0	0	0	0
....................................................................................................................................TCCGAGTGGATCATAAAAT..............................................................................................................	19	6	0.17	1	0	1	0	0	0	0	0	0	0	0
...............................................................................................................................................................................................................................TTGGGAC-AGAATGAAAATAATAGC.............	24	6	0.17	1	0	0	0	0	0	0	0	0	1	0
...........................................................................TTCTTTCCACAGCTGCTGTAGA....................................................................................................................................................................	22	6	0.17	1	0	1	0	0	0	0	0	0	0	0
................................................................................................................................TAAGTCCGAGTGGATCATAAAAT..............................................................................................................	23	6	0.17	1	0	1	0	0	0	0	0	0	0	0
...............................................................................................................................................................................................................................TTGGGAC-AGAATGAAAATAATAGCTCATC........	29	6	0.17	1	0	0	1	0	0	0	0	0	0	0
............................................................................................................................................GATCATAAAATCGTGCAATAGTTG------A..........................................................................................	25	6	0.17	1	0	0	0	0	0	0	0	1	0	0
...............................................................................................................................................................................................................................TTGGGAC-AGAATGAAAATAAT................	21	6	0.17	1	0	1	0	0	0	0	0	0	0	0
...........................................................................................................................................GGATCATAAAATCGTGCAATAGTTG------...........................................................................................	25	6	0.17	1	0	1	0	0	0	0	0	0	0	0
........................................................................................TGCTGTAGAAATATTTTT...........................................................................................................................................................	18	7	0.14	1	0	0	0	0	0	0	0	0	1	0
..........................................................................................................................................TGGATCATAAAATCGTGCAATAGAAA------...........................................................................................	26	7	0.14	1	0	1	0	0	0	0	0	0	0	0
.............................................................................................................................................................................................TCGCTTAGTTGGTTTCGAAGGA..................................................	22	7	0.14	1	0	0	0	0	0	0	0	0	1	0
......................................................................................................................................................................................................TGGTTTCGAAGGGACT------TGATTGGG.................................	24	8	0.13	1	0	1	0	0	0	0	0	0	0	0
......................................................................................................................................................................................................TGGTTTCGAAGGGACT------TGATTGGGA................................	25	8	0.13	1	0	1	0	0	0	0	0	0	0	0
.........................................................................TTTTCTTTCCACAGCTGCTGTA......................................................................................................................................................................	22	8	0.13	1	0	1	0	0	0	0	0	0	0	0
..................................................................................................................................................................................................TAGTTGGTTTCGAAGGGACT------.........................................	20	8	0.13	1	0	0	0	0	0	0	0	0	1	0
...........................................................................................................................................................................................................TCGAAGGGACT------TGATTGGG.................................	19	8	0.13	1	0	0	0	0	0	0	0	0	1	0
..................................................................................................................................................................................................TAGTTGGTTTCGAAGGGACT------A........................................	21	8	0.13	1	0	1	0	0	0	0	0	0	0	0
.......................................................................................................................................................................................................GGTTTCGAAGGGACT------TGATTGG..................................	22	8	0.13	1	0	1	0	0	0	0	0	0	0	0
.........................................................................................................................................................................................................TTTCGAAGGGACT------TGATTGGG.................................	21	8	0.13	1	0	0	0	0	0	1	0	0	0	0
..................................................................................................................................................................................................TAGTTGGTTTCGAAGGGACT------TGA......................................	23	8	0.13	1	0	0	0	0	0	0	0	0	1	0
.....................................................................................................................................................................................................TTGGTTTCGAAGGGACT------TGATTGGG.................................	25	8	0.13	1	0	0	1	0	0	0	0	0	0	0
...........................................................................................................................................................................................................TCGAAGGGACT------TGATTGGGAC-AGAA..........................	25	8	0.13	1	0	0	1	0	0	0	0	0	0	0
........................................................................................................................................................................................................GTTTCGAAGGGACT------TGATTGGGAC-..............................	24	8	0.13	1	0	0	0	0	0	0	1	0	0	0
.........................................................................................................................................................................................................TTTCGAAGGGACT------TGATTGGGA................................	22	8	0.13	1	0	1	0	0	0	0	0	0	0	0
..................................................................................................................................................................................................TAGTTGGTTTCGAAGGGACT------TGAT.....................................	24	8	0.13	1	0	1	0	0	0	0	0	0	0	0
...........................................................................................................................................................................................................TCGAAGGGACT------TGATTGGGAC-AGA...........................	24	8	0.13	1	0	1	0	0	0	0	0	0	0	0
......................................................................................................................................................................................................TGGTTTCGAAGGGACT------TGATTGGGAC-..............................	26	8	0.13	1	0	1	0	0	0	0	0	0	0	0
......................................................................................................................................................................................................TGGTTTCGAAGGGACT------TGATTG...................................	22	8	0.13	1	0	1	0	0	0	0	0	0	0	0
............................................................................................................................................................................................................CGAAGGGACT------TGATTGGG.................................	18	8	0.13	1	0	0	0	0	0	0	0	0	1	0
...........................................................................................................................................................................................................TCGAAGGGACT------TGATTGGGAC-..............................	21	8	0.13	1	0	0	0	0	0	0	0	0	0	1
......................................................................................................................................................................................................TGGTTTCGAAGGGACT------TGATTGGGAC-A.............................	27	8	0.13	1	0	1	0	0	0	0	0	0	0	0
.............................................................................................................................................................................................................GAAGGGACT------TGATTGGGAC-..............................	19	8	0.13	1	0	0	0	0	0	0	0	0	1	0
...............................................................................................................................................................................................................AGGGACT------TGATTGGGAC-A.............................	18	9	0.11	1	0	1	0	0	0	0	0	0	0	0
...................................................................................................................................................................................................................ACT------TGATTGGGAC-AGAATGAA......................	21	9	0.11	1	0	1	0	0	0	0	0	0	0	0
...............................................................................................................................................................................................................AGGGACT------TGATTGGGAC-AGAATGT.......................	24	9	0.11	1	0	1	0	0	0	0	0	0	0	0
...............................................................................................................................................................................................................AGGGACT------TGATTGGGAC-AGAATCC.......................	24	9	0.11	1	0	0	0	0	0	0	0	0	1	0
................................................................................................................................................................................................................GGGACT------TGATTGGGAC-AG............................	18	9	0.11	1	0	1	0	0	0	0	0	0	0	0
..................................................................................................................................................................................................................GACT------TGATTGGGAC-AGAAT.........................	19	9	0.11	1	0	1	0	0	0	0	0	0	0	0
...............................................................................................................................................................................................................AGGGACT------TGATTGGGAC-AGAATGAAAAT...................	28	9	0.11	1	0	1	0	0	0	0	0	0	0	0
................................................................................................................................................................................................................GGGACT------TGATTGGGAC-AGAAAA........................	22	9	0.11	1	0	1	0	0	0	0	0	0	0	0
.................................................................................................................................................................................................................GGACT------TGATTGGGAC-AGAAT.........................	20	9	0.11	1	0	1	0	0	0	0	0	0	0	0
.....................................................................................................................................................................................................................T------TGATTGGGAC-AGAATGAAA.....................	20	9	0.11	1	0	0	0	0	0	0	1	0	0	0
................................................................................................................................................................................................................GGGACT------TGATTGGGAC-AGA...........................	19	9	0.11	1	0	1	0	0	0	0	0	0	0	0
...............................................................................................................................................................................................................AGGGACT------TGATTGGGAC-AGT...........................	20	9	0.11	1	0	0	0	0	1	0	0	0	0	0
.................................................................................................................................................................................................................GGACT------TGATTGGGAC-AGA...........................	18	9	0.11	1	0	1	0	0	0	0	0	0	0	0

droPer2

A----------------------------------------------------------------------GCTTTTCTTTCCACAGCTGCTGTAGAAATATTTTTGCTTTAGTAATAACTGGCCCTATAAGTCCGAGTGGATCATAAAATCGTGCAATAGTTG------ACAATACAA---------CTCGCTTAGTTGGTTTCGAAGGGACT------TGATTGGGAC-AGAATGAAAATAATAGCTTATCCGAAGTGG	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
...............................................................................................................................................................................................................AGGGACT------TGATTGGGAC-AGAATGA.......................	24	20	0.10	2	0	2	0	0	0	0
................................................................................................................................................................................................................GGGACT------TGATTGGGAC-AGAATGA.......................	23	20	0.10	2	0	2	0	0	0	0
...............................................................................................................................................................................................................AGGGACT------TGATTGGGAC-AGAATGAAAA....................	27	20	0.10	2	0	0	0	0	0	2
..................................................................................................................................................................................................................GACT------TGATTGGGAC-AGAATGAAA.....................	23	20	0.10	2	2	0	0	0	0	0
..........................................................................................................................................................................ACAATACAA---------CTCGCTTAGTTGGT...........................................................	23	20	0.05	1	0	1	0	0	0	0
..........................................................................................................................................TGGATCATAAAATCGTGCAATAGTTG------...........................................................................................	26	20	0.05	1	0	1	0	0	0	0
.............................................................................................................................................................................................................GAAGGGACT------TGATTGGGAC-AGAAT.........................	24	20	0.05	1	0	0	0	0	0	1
...........................................................................TTCTTTCCACAGCTGCTGTAG.....................................................................................................................................................................	21	20	0.05	1	0	1	0	0	0	0
................................................................................................................................................................................................................GGGACT------TGATTGGGAC-AGAATGAG......................	24	20	0.05	1	0	1	0	0	0	0
....................................................................................................................................................................................................GTTGGTTTCGAAGGGACT------TGA......................................	21	20	0.05	1	0	0	0	0	0	1

droVir3

A----------------------------------------------------------------------GCTTCTCCTTCCAGATCTGCTGCATAAAGATTTTAGCCTTGGCAATTACCAGCCCTATGAGTCCAAGCGGGTCATACAAGCGAGCTATAGTCG------ATAGC--------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M027 Male-body	M028 Head	M047 Female-body	M061 Embryo	SRR060654 Ovary	SRR060655 Testis	SRR060656 Ovary	SRR060657 Testis	SRR060658 Ovary	SRR060659 Testis	SRR060660 Ovary	SRR060661 Embryo	SRR060662 Embryo	SRR060663 Embryo	SRR060664 Male-body	SRR060665 Female-body	SRR060666 Male-body	SRR060667 Female-body	SRR060668 Male-body	SRR060669 Female-body	SRR060670 Testis	SRR060671 Male-body	SRR060672 Female-body	SRR060673 Ovary	SRR060674 Ovary	SRR060675 Ovary	SRR060676 Ovary	SRR060677 Ovary	SRR060678 Testis	SRR060679 Testis	SRR060680 Testis	SRR060681 Testis	SRR060682 Embryo	SRR060683 Testis	SRR060684 Embryo	SRR060685 Embryo	SRR060686 Embryo	SRR060687 Embryo	SRR060688 Ovary	SRR060689 Testis	SRR1106712 embryo_0-2h	SRR1106713 embryo_0-2h	SRR1106714 embryo_2-4h	SRR1106715 embryo_4-6h	SRR1106716 embryo_4-6h	SRR1106717 embryo_6-8h	SRR1106718 embryo_6-8h	SRR1106719 embryo_8-10h	SRR1106720 embryo_8-10h	SRR1106721 embryo_10-12h	SRR1106722 embryo_10-12h	SRR1106723 embryo_12-14h	SRR1106724 embryo_12-14h	SRR1106725 embryo_14-16h	SRR1106726 embryo_14-16h	SRR1106727 larvae	SRR1106728 larvae	SRR1106729 mixed whole adult body	SRR1106730 embryo_16-30h	V047 Embryo	V053 Head	V116 Male-body	GSM1528803 follicle cells
.........................................................................................GCTGCATAAAGATTTTAGCCTTG.....................................................................................................................................................	23	20	3.00	60	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	54	0	0	0	0	0	0	6	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.................................................................................CCAGATCTGCTGCATAAAGATTTTAGCC........................................................................................................................................................	28	20	1.70	34	0	0	23	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	10	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.................................................................................CCAGATCTGCTGCATAAAGATTTTAGC.........................................................................................................................................................	27	20	1.25	25	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	20	0	0	1	0	0	0	0	0	0	0	4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.................................................................................CCAGATCTGCTGCATAAAGATTT.............................................................................................................................................................	23	20	1.00	20	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	20	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.................................................................................CCAGATCTGCTGCATAAAGATTTTA...........................................................................................................................................................	25	20	0.90	18	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	9	0	0	5	0	0	4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................................................................................GCTGCATAAAGATTTTAGCCTTGGCAAT................................................................................................................................................	28	20	0.85	17	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	13	0	0	1	2	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.................................................................................CCAGATCTGCTGCATAAAGATTTTAGCCT.......................................................................................................................................................	29	20	0.85	17	0	0	12	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................................................................................GCTGCATAAAGATTTTAGCCTTGGC...................................................................................................................................................	25	20	0.65	13	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	10	0	0	0	0	0	0	0	0	0	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.................................................................................CCAGATCTGCTGCATAAAGATTTT............................................................................................................................................................	24	20	0.50	10	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	6	0	0	0	0	0	0	0	4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................................................................................GCTGCATAAAGATTTTAGCCTT......................................................................................................................................................	22	20	0.40	8	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	1	0	0	0	0	0	5	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.....................................................................................................................TACCAGCCCTATGAGTCCAAGCGGGT......................................................................................................................	26	3	0.33	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
................................................................................TCCAGATCTGCTGCATAAAGATTT.............................................................................................................................................................	24	20	0.30	6	0	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................................................................................GCTGCATAAAGATTTTAGCCTTGG....................................................................................................................................................	24	20	0.25	5	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................................................................................GCTGCATAAAGATTTTAGCCTTGT....................................................................................................................................................	24	20	0.25	5	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	2	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................................................................................GCTGCATAAAGATTTTAGCCTTGGCAA.................................................................................................................................................	27	20	0.20	4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
................................................................................TCCAGATCTGCTGCATAAAGATTTTAGC.........................................................................................................................................................	28	20	0.20	4	0	0	4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.................................................................................CCAGATCTGCTGCATAAAGATTTTT...........................................................................................................................................................	25	20	0.15	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...............................................................................TTCCAGATCTGCTGCATAAAGATTT.............................................................................................................................................................	25	20	0.15	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...............................................................................TTCCAGATCTGCTGCATAAAGATTTTT...........................................................................................................................................................	27	20	0.10	2	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
............................................................................TCCTTCCAGATCTGCTGCATAAAG.................................................................................................................................................................	24	20	0.10	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................................................................................GCTGCATAAAGATTTTAGCCTTT.....................................................................................................................................................	23	20	0.10	2	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................................................................................GCTGCATAAAGATTTTAGCCTTTT....................................................................................................................................................	24	20	0.10	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................................................................................GCTGCATAAAGATTTTAGCCT.......................................................................................................................................................	21	20	0.10	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................................................................................GCTGCATAAAGATTTTAGCCTTGGCAT.................................................................................................................................................	27	20	0.10	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.................................................................................CCAGATCTGCTGCATAAAGATTTTCC..........................................................................................................................................................	26	20	0.10	2	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...............................................................................TTCCAGATCTGCTGCATAAAG.................................................................................................................................................................	21	20	0.10	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................................................................TTCTCCTTCCAGATCTGCTGCATAAAGAT...............................................................................................................................................................	29	20	0.10	2	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..............................................................................................................................TATGAGTCCAAGCGGGTCATA..................................................................................................................	21	20	0.10	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
........................................................................................TGCTGCATAAAGATTTTAGCCTT......................................................................................................................................................	23	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................................................................................GCTGCATAAAGATTTTAGCCTTAT....................................................................................................................................................	24	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
................................................................................TCCAGATCTGCTGCATAAAGATT..............................................................................................................................................................	23	20	0.05	1	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
........................................................................................TGCTGCATAAAGATTTTAGCCTTGG....................................................................................................................................................	25	20	0.05	1	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..................................................................................CAGATCTGCTGCATAAAGATTTTAGCC........................................................................................................................................................	27	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1
................................................................................TCCAGATCTGCTGCATAAAGATTTTA...........................................................................................................................................................	26	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................................................................................GCTGCATAAAGATTTTAGCCTTGGCAATT...............................................................................................................................................	29	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.................................................................................CCAGATCTGCTGCATAAAGATTTTGT..........................................................................................................................................................	26	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.................................................................................CCAGATCTGCTGCATAAAGATTTTAG..........................................................................................................................................................	26	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..........................................................................................CTGCATAAAGATTTTAGCCTTGGCAAT................................................................................................................................................	27	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.................................................................................CCAGATCTGCTGCATAAAGATTC.............................................................................................................................................................	23	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.......................................................................................................................................AAGCGGGTCATACAAGCGAGCTAA......................................................................................................	24	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
........................................................................................................................................AGCGGGTCATACAAGCGAGC.........................................................................................................	20	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
............................................................................TCCTTCCAGATCTGCTGCATAAAGATTT.............................................................................................................................................................	28	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................................................................................GCTGCATAAAGATTTTAGCCTTGGCAC.................................................................................................................................................	27	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
........................................................................................TGCTGCATAAAGATTTTAGCCTTGGCA..................................................................................................................................................	27	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.....................................................................................ATCTGCTGCATAAAGATTTTAGCCTTA.....................................................................................................................................................	27	20	0.05	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.....................................................................................ATCTGCTGCATAAAGATTTTAGCC........................................................................................................................................................	24	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.................................................................................CCAGATCTGCTGCATAAAGATA..............................................................................................................................................................	22	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................................................................................GCTGCATAAAGATTTTAGCCTTGGT...................................................................................................................................................	25	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................................................................................GCTGCATAAAGATTTTAGCCTTGGCTT.................................................................................................................................................	27	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
....................................................................................................................................TCCAAGCGGGTCATACAAGCG............................................................................................................	21	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..............................................................................CTTCCAGATCTGCTGCATAAAGATTTTC...........................................................................................................................................................	28	20	0.05	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................................................................................GCTGCATAAAGATTTTAGCCTTGTT...................................................................................................................................................	25	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................................................................................GCTGCATAAAGATTTTAGCCTTTTT...................................................................................................................................................	25	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
....................................................................................GATCTGCTGCATAAAGATTT.............................................................................................................................................................	20	20	0.05	1	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
....................................................................................GATCTGCTGCATAAAGATTTTAGCCTT......................................................................................................................................................	27	20	0.05	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................................................................................GCTGCATAAAGATTTTAGCCTTGTAT..................................................................................................................................................	26	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.................................................................................CCAGATCTGCTGCATAAAGATT..............................................................................................................................................................	22	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
................................................................................TCCAGATCTGCTGCATAAAGA................................................................................................................................................................	21	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..........................................................................TCTCCTTCCAGATCTGCTGCAT.....................................................................................................................................................................	22	20	0.05	1	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

droMoj3

A----------------------------------------------------------------------GTTTATACCTCCAGAGTTGTTGCAAGAAAATTTTAGCCTTCGTTATTACTGGGCCGACCAGGCCGAGGGGATCATAAAACCGAGCAATAGACG------AGAGAACAG---------AGCGCCTGGAGGGCTTAG-------AGGCTGAATTCAGGGCTAGATATGAGAAAAGAAGTTGATCAGATGCCG	Size	Hit Count	Total Norm	Total	M046 Female-body	V041 Embryo	V049 Head	V056 Head	M060 Embryo	V110 Male-body
..............................................................................................................................................................TAGACG------AGAGAACAG---------AGCGCCTGGAG..............................................................	26	20	22.90	458	457	1	0	0	0	0
...........................................................................................................................................................CAATAGACG------AGAGAACAG---------AGCGCC...................................................................	24	20	4.20	84	0	0	0	0	84	0
..............................................................................................................................................................TAGACG------AGAGAACAG---------AGCGCCTGGAGGG............................................................	28	20	1.65	33	32	0	0	0	0	1
...........................................................................................................................................................CAATAGACG------AGAGAACAG---------AGCGCCT..................................................................	25	20	1.10	22	0	0	0	0	22	0
...............................................................................................................................................................AGACG------AGAGAACAG---------AGCGCCTGGA...............................................................	24	20	0.80	16	16	0	0	0	0	0
..........................................................................................................................................................................................................................................ATGAGAAAAGAAGTTGATCAGATG...	24	20	0.70	14	14	0	0	0	0	0
...........................................................................................................................................................CAATAGACG------AGAGAACAG---------AGCGCCTG.................................................................	26	20	0.45	9	9	0	0	0	0	0
........................................................................................................................TGGGCCGACCAGGCCGAGGGGATCA....................................................................................................................	25	20	0.35	7	7	0	0	0	0	0
...........................................................................................TGCAAGAAAATTTTAGCCTTCGTTA.................................................................................................................................................	25	13	0.31	4	4	0	0	0	0	0
...........................................................................................................................................................CAATAGACG------AGAGAACAG---------AGC......................................................................	21	20	0.30	6	0	0	0	0	6	0
........................................................................................................................TGGGCCGACCAGGCCGAGGGGATC.....................................................................................................................	24	20	0.30	6	6	0	0	0	0	0
..............................................................................................................................................................TAGACG------AGAGAACAG---------AGCGCCTGGAA..............................................................	26	20	0.25	5	2	0	0	0	3	0
..............................................................................................................................................................TAGACG------AGAGAACAG---------AGCGCCTGGAC..............................................................	26	20	0.25	5	0	0	0	0	5	0
...........................................................................................TGCAAGAAAATTTTAGCCTTCGTTAT................................................................................................................................................	26	13	0.15	2	2	0	0	0	0	0
.........................................................................................................................................................................................................................................TATGAGAAAAGAAGTTGATCAGATGC..	26	20	0.15	3	0	0	0	0	3	0
..........................................................................................................................................................................................................TTAG-------AGGCTGAATTCAGGG.................................	19	20	0.15	3	3	0	0	0	0	0
..............................................................................................................................................................TAGACG------AGAGAACAG---------AGC......................................................................	18	20	0.15	3	3	0	0	0	0	0
................................................................................................................................................................GACG------AGAGAACAG---------AGCGCCTGGAGGG............................................................	26	20	0.15	3	0	0	0	0	3	0
.......................................................................................................................................................................................................................................GATATGAGAAAAGAAGTTGATCAGATGC..	28	20	0.15	3	0	0	0	0	3	0
..............................................................................................................................................................TAGACG------AGAGAACAG---------AGCGCCTG.................................................................	23	20	0.15	3	0	0	0	0	0	3
..........................................................................................................................................................................................................TTAG-------AGGCTGAATTCAGGGCTAGATA..........................	26	20	0.10	2	2	0	0	0	0	0
.....................................................................................................................................................................................................................AGGCTGAATTCAGGGCTA..............................	18	20	0.10	2	2	0	0	0	0	0
.....................................................................................................................TACTGGGCCGACCAGGCCGAGGGG........................................................................................................................	24	20	0.10	2	2	0	0	0	0	0
.........................................................................................GTTGCAAGAAAATTTTAGCCTTCGTTA.................................................................................................................................................	27	13	0.08	1	1	0	0	0	0	0
...............................................................................................AGAAAATTTTAGCCTTCGTT..................................................................................................................................................	20	16	0.06	1	1	0	0	0	0	0
...........................................................................................................................................................................GAGAACAG---------AGCGCCTGGAGGGC...........................................................	22	20	0.05	1	0	0	0	0	1	0
........................................................................................................................................................................................................................................ATATGAGAAAAGAAGTTGATCAGATC...	26	20	0.05	1	1	0	0	0	0	0
............................................................................................................................................................AATAGACG------AGAGAACAG---------AGCGCCTGG................................................................	26	20	0.05	1	0	0	0	0	1	0
...............................................................................................................................................................AGACG------AGAGAACAG---------AGCGCCTGGAGGG............................................................	27	20	0.05	1	1	0	0	0	0	0
..................................................................................................................................................................................................................................................AGAAGTTGATCAGATGCC.	18	20	0.05	1	0	0	0	0	1	0
..........................................................................................................................................................................................................TTAG-------AGGCTGAATTCAGGGCTAG.............................	23	20	0.05	1	1	0	0	0	0	0
.....................................................................................................................TACTGGGCCGACCAGGCCGAGG..........................................................................................................................	22	20	0.05	1	1	0	0	0	0	0
.......................................................................................................................................................................................................................................GATATGAGAAAAGAAGTT............	18	20	0.05	1	1	0	0	0	0	0
...................................................................................................................................................................G------AGAGAACAG---------AGCGCCTGGAGGGC...........................................................	24	20	0.05	1	0	0	0	0	1	0
...............................................................................................................................................................AGACG------AGAGAACAG---------AGCGCCT..................................................................	21	20	0.05	1	0	0	0	1	0	0
..............................................................................................................................................................TAGACG------AGAGAACAG---------AGCGCCTGGC...............................................................	25	20	0.05	1	1	0	0	0	0	0
............................................................................................................................................................AATAGACG------AGAGAACAG---------AGCGCCT..................................................................	24	20	0.05	1	0	0	0	0	1	0
.............................................................................................................................................................................................................................TTCAGGGCTAGATATGAGAAAAGAAG..............	26	20	0.05	1	1	0	0	0	0	0
........................................................................................................................TGGGCCGACCAGGCCGAGGGGATCATAA.................................................................................................................	28	20	0.05	1	0	0	0	0	0	1
...............................................................................................................................................................................................GCCTGGAGGGCTTAG-------AGGCTG..........................................	21	20	0.05	1	0	0	0	0	0	1
....................................................................................................................................................................................................GAGGGCTTAG-------AGGCTGAATTCAGGGC................................	26	20	0.05	1	1	0	0	0	0	0
................................................................................................................................................................................................................................................AAAGAAGTTGATCAGATGCCG	21	20	0.05	1	1	0	0	0	0	0
..............................................................................................................................................................TAGACG------AGAGAACAG---------AGCGCCTGA................................................................	24	20	0.05	1	1	0	0	0	0	0
...........................................................................................................................................................................................................................................TGAGAAAAGAAGTTGATCAG......	20	20	0.05	1	1	0	0	0	0	0
...............................................................................................................................................................................................................................CAGGGCTAGATATGAGAAAAG.................	21	20	0.05	1	0	0	0	1	0	0
.......................................................................................................................................................................................................................................GATATGAGAAAAGAAGTTGATCAGAT....	26	20	0.05	1	0	0	0	0	1	0
............................................................................................................................................................AATAGACG------AGAGAACAG---------AGCGCCG..................................................................	24	20	0.05	1	0	0	0	0	1	0
.......................................................................................................................................................................................................................................GATATGAGAAAAGAAGTTGATCAGATG...	27	20	0.05	1	0	1	0	0	0	0
..............................................................................................................................................................TAGACG------AGAGAACAG---------AGCGCCC..................................................................	22	20	0.05	1	1	0	0	0	0	0
....................................................................................................................TTACTGGGCCGACCAGGCCGAGG..........................................................................................................................	23	20	0.05	1	1	0	0	0	0	0

droGri2

-------------------------------------------------------------------------TTTGCCTTCCAGAGTTGCTGCAGGAATATTTTGCACCTAGCAATTGCAGGTCCAATAAGACCAAGAGGATCATAGATACACGCAATCGTGT------ACAAGATGG---------ATCGCTTGGAGAGTTTTG-------AATTTGGGCGAACAGGTGACAACAAAAACAAAAGATTATCCGAAACTG	Size	Hit Count	Total Norm	Total	M041 Female-body	V109 Male-body
No Reads

dsi_25671

2l:23250564-23250713 -

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-0.4	-0.4	-0.1