dsi_23971

----------------------------------------------------------------------------------------------------------################################################################################################################################################ GGAAAGAAAATACTTACGCAGCACTCCCACGCAAATACAGCAAAACATATGTTTCGGTATTTTTTATGTATTGGCACGCAGATTTCAGCGCTTAGTGTAGTTAGGTCACACACCAACACTATTCGTGCGTAGTTTTCATTTATTTTGCTCCGCTTTCCTTCGCTGTTTTCACACCCACGATTCGCGAAAAAAACACCCAGGAATTGTGTCCAAACATTGGCAAACATTTTGTGTACCCCCGCGGGCATAA *******************************************************************************************************.....................(((.(((.............))).)))............***********************************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	SRR618934 dsim w501 ovaries	M023 head	M025 embryo	M053 female body	O002 Head	O001 Testis	SRR553486 Makindu_3 day-old ovaries	SRR553488 RT_0-2 hours eggs	SRR902009 testis	SRR553487 NRT_0-2 hours eggs
...................................................................................................................................................CTCCGCTTTCCTTCGCTG.....................................................................................	18	0	1	4.00	4	0	2	2	0	0	0	0	0	0	0
...........................................................................................................................................TTATTTTGCTCCGCTTTCCTTCGCTGTT...................................................................................	28	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0
...................................................................................................................................................CTCCGCTTTCCTTCGCTGT....................................................................................	19	0	1	1.00	1	0	0	0	1	0	0	0	0	0	0
.........................................................................................................TCACACACCAACACTATTCGTGC..........................................................................................................................	23	0	1	1.00	1	0	0	0	0	0	1	0	0	0	0
................................................................................................................................................................................ACGATTCGCGAAAAAA..........................................................	16	0	1	1.00	1	0	0	0	0	0	0	1	0	0	0
...........................................................................ACGCAGATTTCAGCGCTT.............................................................................................................................................................	18	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0
..............................................................................................................CACCAACACTATTCGTGCGT........................................................................................................................	20	0	1	1.00	1	0	0	0	1	0	0	0	0	0	0
....................................................................................................TTAGGTCACACACCAACAC...................................................................................................................................	19	0	1	1.00	1	0	0	0	0	1	0	0	0	0	0
..........................................................................................................................TCGTGCGTAGTTTTCATTTATTTTGC......................................................................................................	26	0	1	1.00	1	0	0	0	0	0	0	0	1	0	0
..................................................................................................................................................................................................................GAAGCATTGGCAAACATTTTG...................	21	2	4	0.25	1	0	0	0	0	0	0	0	0	1	0
........................................................................................................................................................................................................................TAGGGAAACATTTTGTGGACC.............	21	3	9	0.11	1	1	0	0	0	0	0	0	0	0	0
...................................................................................................................................................................................................................AAACATTGGAAAACATTT.....................	18	1	12	0.08	1	0	0	0	0	1	0	0	0	0	0
......................................................................................................................CTATTCGAGCGTCGTT....................................................................................................................	16	2	20	0.05	1	0	0	0	0	0	0	0	0	0	1
.................................................................................................................................................................................................CACCCCGGAATTGTTT.........................................	16	2	20	0.05	1	0	0	0	0	0	0	0	0	0	1

CCTTTCTTTTATGAATGCGTCGTGAGGGTGCGTTTATGTCGTTTTGTATACAAAGCCATAAAAAATACATAACCGTGCGTCTAAAGTCGCGAATCACATCAATCCAGTGTGTGGTTGTGATAAGCACGCATCAAAAGTAAATAAAACGAGGCGAAAGGAAGCGACAAAAGTGTGGGTGCTAAGCGCTTTTTTTGTGGGTCCTTAACACAGGTTTGTAACCGTTTGTAAAACACATGGGGGCGCCCGTATT ***********************************************************************************.....................(((.(((.............))).)))............*******************************************************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	SRR902009 testis	M023 head	O001 Testis	SRR618934 dsim w501 ovaries	GSM343915 embryo	SRR553488 RT_0-2 hours eggs	SRR902008 ovaries	SRR553485 Chicharo_3 day-old ovaries
...............................................................................................................................................................................GTGCTAAGCGCTTTTTTTGTG......................................................	21	0	1	1.00	1	1	0	0	0	0	0	0	0
......................................................................................................................................................................TTTGTGTGGGTGCTAAGCGCTTT.............................................................	23	3	1	1.00	1	1	0	0	0	0	0	0	0
..................CTCGTGAGGATGCGTTTATG....................................................................................................................................................................................................................	20	2	1	1.00	1	0	1	0	0	0	0	0	0
...................................................................................................CAATCCAGTGTGTGGTTGTGAT.................................................................................................................................	22	0	1	1.00	1	0	0	1	0	0	0	0	0
...............................................................................................................................................................................................TTGTGGGGCCGTAACACA.........................................	18	2	7	0.14	1	0	0	0	0	1	0	0	0
...................................................................................................................................................................................TAAGCGCTTTTTTCGTG......................................................	17	1	7	0.14	1	0	0	0	1	0	0	0	0
...................................................................................................................TGAGATAAGCGCGGATCAA....................................................................................................................	19	3	10	0.10	1	0	0	0	0	0	1	0	0
...........................................................................................................................................AAAAAAACGAGGCGAATGGAT..........................................................................................	21	3	11	0.09	1	0	0	0	0	0	0	1	0
.....................GTGAAGGTGCGTTTA......................................................................................................................................................................................................................	15	1	18	0.06	1	0	0	0	0	0	0	0	1

Species	Coordinate	ID	Alignment
droSim2	3l:1479224-1479473 +	dsi_23971	G--GAA--AGAAAATACT------TACGCAGCACTCCCACGCAAA-TACAGCAAAACATATGTTTCGGTATTTTTTATGTATTGGCACGCAGATTTCAG------------------------------------------------C----------------------GCTTAGTGTAGTTAGGTCACACACCAACACTA--------T---TCGTGCGTAGTTTTCATTTATTTTGCTCC-GCTTTCCTTCGCTGTTTTCACACCCACGATTCGCGAAAAAAAC--ACCCAGGAATTGTGT-CCAAACATTGGC-AAA--------------------------------CATT-TTG-TGTAC-----------C-CCCGC--GGGC--ATAA-
droSec2	scaffold_2:1595544-1595794 +		G--GAA--AGAAAATACA------TACGCAGCACTCCCACGCAAA-TACTGCAAAACATATGTTTCGGTATTTTTTATGTATTGGCACGCAGATTTCAG------------------------------------------------C----------------------GCTTAGTGTAGTTAGGTCACACACCAACACGA--------T---TCGTGCGTAGTTTTCATTTATTTTGCTCC-GCTTTCCTTCGCTGTTTTCAAACCCACGATTCGCGAAAAAAAC--ACCCAGGAATTGTGT-CCAAACATTGGC-AAA--------------------------------CATT-TTG-TGTAC-----------CCCCCGC--GGGC--ATAA-
dm3	chr3L:1569094-1569343 +		G--AAA--AGAAAATAAC------TACGCAGCACTCCCACGCAAA-TACTGTAAAACATATGTTTCGGTATTTTTTATGTATTGGCACGCAGATTTTAG------------------------------------------------C----------------------GCTTAGTGTAGTTAGGTCACACACCAACACGA--------T---TCGTGCGTAGTTTTCATTTATTTTGCTCC-GCTTTCCTTCGCTGTTTTCACACCCACGATCCGCGAAAAAAAC--ATCCAGGAATCGTGT-TCAAACATTGGC-AAA--------------------------------CATA-TTG-CGTAC-----------C-GCCGC--GGCC--ATAA-
droEre2	scaffold_4784:1547797-1548054 +		T--AAAAAAGACATT-CCAAACACTACGCAACACTCCCCCGCAAA-TACCGCAAAACAGATGTTTCGGTATTTTTTATGTATTGGCACGCAGATTTCAG------------------------------------------------C----------------------ATTTAGTGTAGTTAGGTCACACACCAACACGA--------T---TTGTGCGTAGTTTTCATTTATTTTGCTCC-GCTTTCCTTCGCTGTTTTCACACCCACGATCCGCGAAAAA-AAACACCAAGGAATTGTGT-CTAAACATTGGC-AAA--------------------------------CATA-TTG-CGTAC-----------C-CCCGC--GGGC--ATAA-
droYak3	3L:1519286-1519542 +		T--GAACAAGACATTCC-GCATACTACGCAGCACTCCCCCGCAAA-TACTGCAAAACAGATGTTTCGGTATTTTTTATGTATTGGCACGCAGATTTCAG------------------------------------------------T----------------------GCTTAGTGTAGTTAGGTCACACACCAACACGA--------T---TCGTGCGTAGTTTTCATTTATTTTGCTCC-GCTTTCCTTCGCTGTTTTCACACCCACGATCCGCGAAAAAAAC--AGCCAGGAATTGTGT-CCAAACATTAGC-AAA--------------------------------CATA-TTG-CGTAC-----------C-CCCGC--GGGC--ATAA-
droEug1	scf7180000409007:385702-386006 +		T--AAA-----AAATACC--ATTCGACGCAGGACTGCCCTGCACA-TACCTTAAAACATATGGTTCGGTATTTCTTGTGTATTGGTATGCAGATTCCCGTGTATGTT---AAAATGTC--------TGTTAAAAATAAGTGTGACCGCGCTGGTGCATATTTCTAATAACCTGTGTTGCCGTTTGGTCACA--CGGACCCGA--------A---TCGTGCGTAATTTCCATTTATTTTGCTCC-GCTTTC-TTCGCTGTTTTCACACCCACGATCCGCGAAAAA-A---TCCCAGGAATTG-GT-ACAAACATCGGC-AAA--------------------------------CATA-TTGGTGTTC-----------G-TTGGC--GGGC--ATAA-
droBia1	scf7180000300910:1681137-1681385 +		-----------------------------------------CACA-TACCGCAAAACATATGTTTCGGTATTTTTTGTGTATTGGCATTCAGTTTAT---------TACAAAAATATCGAAATATATT---------------------TTGGT--ATATTTTTC-----AC-TACAGTAGTTAGGTCACACCCCACGTATA--------T---TCGTGCGTAATTTTCATTTATTTTGCTCC-GCTTTCCTCCGCTGTTTTCTCACCCACGGTTCGCGAAAAA-----CCCCAGGAATCGTTGGCAAAACAGTGGC-AAA--------------------------------CATA-TTG-TGCGC-----------G-TTCGC--GGAC--ATAA-
droTak1	scf7180000415095:231716-231959 -		GCCAAA--AGAAAATACC-----------AACACGCCCCCGCACA-TACCGCA-AACATATGTTTCGGTATTTTTTGTGTATTGGCACGCAGAATTCTC------------------------------------------------G----------------------GCTTGTTGTAGTTAGGTCACACACCGAAACAA--------A---TCGTTCGTAATTTTCATTTATTTTGCTCC-GCTTTCCT-CGCTGTTTTCACACCCACGGTTCGCGAAAAA-AC---CCCTGGAATCGTTTGCCAAACATTGGC-AAA--------------------------------CATA-TTG-TGTGC-----------A-TTCGC--GGGC--ATAA-
droEle1	scf7180000491249:4022607-4022845 -		C--TAA--GGAAAATACC------AAGTATACACTCC----------ACCGCAAAACATATGTTTCGGTATTTTTTATGTATTGGCATGCAGATTTCCG------------------------------------------------C----------------------AGTTATTGTAGTTAGGTCACACACCGACACAA--------A---TCGTGCGTAATTTTCATTTATTTTGCTCC-GCTTTCCTTCGCTGTTTTCACACCCACAATCGGGAAAAAT-AC--ACCCAGGAGTTGGGA-CCAAACATTGGA-CAA--------------------------------CATA-TTG-TGTAC-----------G-TTCGC--GGGC--ATA--
droRho1	scf7180000776850:386392-386642 -		CCCAGA--CGAAAATACC------AAGTGTTCACACCCCCGCACA-TACCGCAAAACATATGTTTTGGTATTTTTTGTGTATTGGCATGCATATTTCCG------------------------------------------------C----------------------GCTCAGTGTAGTTAGGTCACACACCGACACAA--------A---TCGTGCGTAATTTTCATTTATTTTGCTCC-GCTTTCCTTCGCTGTTTTCACACCCACTATCGGCAAAAAT-AA--ATCCAGGAATTGGGC-CCAAACATTGGC-CAA--------------------------------CACA-TTG-TGTGC-----------G-TTCGC--GGGC--ATAG-
droFic1	scf7180000454113:516582-516822 -		C--ACA--AGAACGTACT------TGTC-----C-ACCCTGTACTCTCCCGCA-AACATATGGTTAGGTATTTTTTGTGTATTGGTATGCTGATTTCCG------------------------------------------------C----------------------GCTTAGCGCAGTTTGGTCACACACCGCCACAA--------A---TCGTGCGTAATTTTCATTTATTTTGTTCC-GCTTTCCTTCGCTGTTTTCACACCCACGGTCCGCGAAAAC-AC---TTCAGAAATTGTGC-CCAAACATTGGC-ATA--------------------------------CATA-TTG-AGTGC-----------A-TTTGT--GGTT--ATA--
droKik1	scf7180000302510:932716-932914 -		----------------------------------------------------------------TTGGTATTTTTTC------AAAAAGAAGGGTTTAT------------------------------------------------C----------------------CCTGGGTTGGGTACGGTCACATTGCAACGAAAACAACAACTCCGTAACTCGTAATTTCGATTTATTTTGC------TTTCATTCGCTATTTTCACACCCACAATCTGCGAAAAT-AA--GGC-------------CATAACCGTGGC-ACG--------------------------------CATA-TTG-TGCGTTCTATCAGTGTG-TTCGCGCGGTCCAACAA-
droAna3	scaffold_13337:12438196-12438328 +		---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CGTAATTTCCATTTATTTTGCTCT-GCTTTCTTTCGCCGTTTTTCCACCCACAATCCGCAGAAAA-AT--ATCCCGAATTTGTG--CGAAACGGTGGCCCCA--------------------------------CATA-TTG-TGCGTATAATTTCTTAG-TTCAC--GGTG--TTAA-
droBip1	scf7180000396589:1549324-1549457 -		---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CGTAATTTCCATTTATTTTGCTCT-GCTTTCTTTCGCCGTTTTTCCACCCACAATCCGCAGAAAT-AT--ATCCCGATTTTGTG--CGAAACGGTGGCCCCA--------------------------------CATA-TTG-TGCGTGTAGTTTCTTAG-TTCGC--GATA--ATAAA
dp5	XR_group6:6349977-6350137 -		----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TCGTGCGTAATTTCCATTTATTTTGCTCCTGCTTTCATCAAATGTTTTCACACACTTGAACTGCATAAAT-AA--TTACAGAAATTGTGT-AAAGACCTTG-CAACGAGTGCACTAATCACGTATTAAATATTTGTCTTGGTGTTCG-CTTTC-----------G-TTTTC--GGGC--ATAA-
droPer2	scaffold_82:248431-248591 +		----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TCGTGCGTAATTTCCATTTATTTTGCTCCTGCTTTCATCAAATGTTTTCACACACTTGAACTGCATAAAT-AA--TTACAGAAATTGTGT-AAAGACCTTG-CAACGAGTGCACTAATCACGTATTAAATATTTGCCTTGGTGTTCG-CTTTC-----------G-TTTTC--GGGC--ATAA-

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droSim2

G--GAA--AGAAAATACT------TACGCAGCACTCCCACGCAAA-TACAGCAAAACATATGTTTCGGTATTTTTTATGTATTGGCACGCAGATTTCAG------------------------------------------------C----------------------GCTTAGTGTAGTTAGGTCACACACCAACACTA--------T---TCGTGCGTAGTTTTCATTTATTTTGCTCC-GCTTTCCTTCGCTGTTTTCACACCCACGATTCGCGAAAAAAAC--ACCCAGGAATTGTGT-CCAAACATTGGC-AAA--------------------------------CATT-TTG-TGTAC-----------C-CCCGC--GGGC--ATAA-	Size	Hit Count	Total Norm	Total	M023 Head	M024 Male-body	M025 Embryo	M053 Female-body	O001 Testis	O002 Head	SRR553485 Ovary	SRR553486 Ovary	SRR553487 Ovary	SRR553488 Ovary	SRR618934 Ovary	V044 Embryo	SRR902008 Ovary	SRR902009 Testis	SRR1275487 Male larvae	SRR1275485 Male prepupae	SRR1275483 Male prepupae
...............................................................................................................................................................................................................................................CTCC-GCTTTCCTTCGCTG............................................................................................................................................	18	1	4.00	4	2	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.......................................................................................................................................................................................................................................TTATTTTGCTCC-GCTTTCCTTCGCTGTT..........................................................................................................................................	28	1	1.00	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
...............................................................................................................................................................................................................................................CTCC-GCTTTCCTTCGCTGT...........................................................................................................................................	19	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0
..........................................................................................................................................................................................TCACACACCAACACTA--------T---TCGTGC..................................................................................................................................................................................	23	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0
.............................................................................................................................................................................................................................................................................ACGATTCGCGAAAAAA.................................................................................................................	16	1	1.00	1	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0
......................................................................................ACGCAGATTTCAG------------------------------------------------C----------------------GCTT................................................................................................................................................................................................................................	18	1	1.00	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
...............................................................................................................................................................................................CACCAACACTA--------T---TCGTGCGT................................................................................................................................................................................	20	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0
.....................................................................................................................................................................................TTAGGTCACACACCAACAC......................................................................................................................................................................................................	19	1	1.00	1	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0
......................................................................................................................................................................................................................TCGTGCGTAGTTTTCATTTATTTTGC..............................................................................................................................................................	26	1	1.00	1	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0

droSec2

G--GAA--AGAAAATACA------TACGCAGCACTCCCACGCAAA-TACTGCAAAACATATGTTTCGGTATTTTTTATGTATTGGCACGCAGATTTCAG------------------------------------------------C----------------------GCTTAGTGTAGTTAGGTCACACACCAACACGA--------T---TCGTGCGTAGTTTTCATTTATTTTGCTCC-GCTTTCCTTCGCTGTTTTCAAACCCACGATTCGCGAAAAAAAC--ACCCAGGAATTGTGT-CCAAACATTGGC-AAA--------------------------------CATT-TTG-TGTAC-----------CCCCCGC--GGGC--ATAA-	Size	Hit Count	Total Norm	Total	M054 Female-body	V113 Male-body	V114 Embryo	V115 Head
........................................................................................................................................................................................................GA--------T---TCGTGCGTAGTTTTCATTTATT..................................................................................................................................................................	25	1	1.00	1	0	1	0	0
........................................................................................................................................................................................................GA--------T---TCGTGCGTAGTTTTC.........................................................................................................................................................................	18	1	1.00	1	0	0	0	1
.......................................................................................................................................................................................................CGA--------T---TCGTGCGTAGTTTTCATTTAT...................................................................................................................................................................	25	1	1.00	1	0	0	0	1
..................................................................................................................................................................................................................................TTCATTTATTTTGCTCC-G.........................................................................................................................................................	18	1	1.00	1	0	0	0	1
..................................................................................................................................................................................................................................TTCATTTATTTTGCTCC-GCTTTCCTT.................................................................................................................................................	26	1	1.00	1	1	0	0	0
..............................................................................................................................................................................AGTGTAGTTAGGTCACACACCAACACG.....................................................................................................................................................................................................	27	1	1.00	1	0	0	1	0
...................................................................................................................................................................................................................................................................TTTCAAACCCACGATTCGC........................................................................................................................	19	1	1.00	1	0	0	1	0
........................................................................................................................................................................................................GA--------T---TCGTGCGTAGTTTTCA........................................................................................................................................................................	19	1	1.00	1	0	0	1	0
.......................................................................................................................................................................................................................................................................AAACCCACGATTCGCGAAA....................................................................................................................	19	1	1.00	1	1	0	0	0
..............................................................................................................................................................................AGTGTAGTTAGGTCACACACC...........................................................................................................................................................................................................	21	1	1.00	1	0	0	1	0
............................................................................................................................................................................................ACACACCAACACGA--------T---TCGTGCGTAGTTT...........................................................................................................................................................................	28	1	1.00	1	0	0	1	0
..........................................................................................................................................................................................TCACACACCAACACGA--------T---TCGTGCG.................................................................................................................................................................................	24	1	1.00	1	0	0	0	1
..................................................................................................................................................................................................................................TTCATTTATTTTGCTCC-GCTTT.....................................................................................................................................................	22	1	1.00	1	0	0	0	1
.........................................................................................................................................................................................................A--------T---TCGTGCGTAGTTTTCA........................................................................................................................................................................	18	2	0.50	1	0	0	0	1

dm3

G--AAA--AGAAAATAAC------TACGCAGCACTCCCACGCAAA-TACTGTAAAACATATGTTTCGGTATTTTTTATGTATTGGCACGCAGATTTTAG------------------------------------------------C----------------------GCTTAGTGTAGTTAGGTCACACACCAACACGA--------T---TCGTGCGTAGTTTTCATTTATTTTGCTCC-GCTTTCCTTCGCTGTTTTCACACCCACGATCCGCGAAAAAAAC--ATCCAGGAATCGTGT-TCAAACATTGGC-AAA--------------------------------CATA-TTG-CGTAC-----------C-GCCGC--GGCC--ATAA-	Size	Hit Count	Total Norm	Total	GSM154618 Ovary	GSM154620 Ovary	GSM154621 Ovary	GSM154622 Ovary	GSM180328 Head	GSM180329 Mixed-body	GSM180330 Embryo	GSM180331 Embryo	GSM180332 Embryo	GSM180333 Embryo	GSM180334 Larvae	GSM180335 Disc	GSM180336 Pupae	GSM180337 S2	GSM231091 Ovary	GSM239041 Head	GSM239050 Head	GSM239051 S2	GSM239052 S2	GSM246084 Head	GSM266765 S2	GSM280082 Ovary	GSM280083 Ovary	GSM280084 Ovary	GSM280085 Testis	GSM280086 Ovary	GSM280087 S2-NP	GSM280088 S2-NP	GSM312995 Head	GSM313160 Head	GSM313161 Head	GSM313162 Head	GSM313163 Head	GSM313164 Head	GSM313165 Head	GSM343287 Embryo	GSM379050 Ovary	GSM379051 Ovary	GSM379052 Ovary	GSM379053 Ovary	GSM379054 Ovary	GSM379055 Ovary	GSM379056 Ovary	GSM379057 Ovary	GSM379058 Ovary	GSM379059 Ovary	GSM379060 Ovary	GSM379061 Ovary	GSM379062 Ovary	GSM379063 Ovary	GSM379064 Ovary	GSM379065 Ovary	GSM379066 Ovary	GSM379067 Ovary	GSM424739 S2	GSM424740 S2	GSM424741 S2	GSM467729 Female-body	GSM467730 Female-body	GSM467731 Female-body	M002 CS	M003 Male-body	M004 Male-body	M005 ML-DmD32	M006 ML-DmD32	M029 OSC	M030 OSC	M031 OSC	M032 S2-R+	M033 S2-R+	M034 S2-R+	M035 S2-R+	M036 S2-R+	M037 S2-R+	M038 S2-R+	M039 S2-R+	M048 S2-R+	OSS2 OSS	OSS6 OSS	OSS7 OSS	OSS8 OSS	S1 Head	S10 Embryo	S11 Embryo	S12 Embryo	S13 Embryo	S14 Embryo	S15 S2-GMR	S16 Kc	S17 Larvae	S18 Larvae	S19 Pupae	S2 Head	S20 Pupae	S21 Disc	S22 Head	S23 Female-body	S24 Male-body	S25 Larvae	S26 Larvae	S27 Pupae	S28 Pupae	S29 Head	S3 Male-body	S30 S2-GMR	S31 S2	S32 S2	S33 Kc	S34 Kc	S35 Kc	S4 Female-body	S5 Disc	S6 Embryo	S7 Embryo	S8 S2	S9 Embryo	SRR001337 Head	SRR001338 Head	SRR001339 Head	SRR001340 Head	SRR001341 Head	SRR001342 Head	SRR001343 Head	SRR001344 Head	SRR001345 Head	SRR001346 Head	SRR001347 Head	SRR001348 Head	SRR001349 Head	SRR001664 Head	SRR010951 Ovary	SRR010952 Ovary	SRR010953 Ovary	SRR010954 Ovary	SRR010955 Ovary	SRR010956 Ovary	SRR010957 Ovary	SRR010958 Ovary	SRR010959 Ovary	SRR010960 Ovary	SRR014268 Embryo	SRR014269 Embryo	SRR014270 Embryo	SRR014271 Embryo	SRR014272 Embryo	SRR014273 Ovary	SRR014274 Ovary	SRR014275 Ovary	SRR014276 Ovary	SRR014277 Ovary	SRR014278 Ovary	SRR014279 Ovary	SRR014280 Ovary	SRR014281 Ovary	SRR014282 Ovary	SRR023197 S2	SRR023399 S2	SRR023400 S2	SRR023402 S2	SRR023407 S2	SRR029028 S2	SRR029029 S2	SRR029030 S2	SRR029031 S2	SRR029032 S2	SRR029033 S2	SRR029608 Head	SRR029633 Head	SRR030145 gb3	SRR031692 Head	SRR031693 Head	SRR031694 Head	SRR031696 Head	SRR031697 Head	SRR031698 Head	SRR031699 Head	SRR031700 Head	SRR031701 Head	SRR031702 Head	SRR031703 Head	SRR031704 Head	SRR032092 S2	SRR032093 S2	SRR032094 S2	SRR032095 S2-NP	SRR032096 S2-NP	SRR032152 Head	SRR060643 Testis	SRR060644 Ovary	SRR060645 Testis	SRR060646 Ovary	SRR060647 Ovary	SRR060648 Ovary	SRR060649 Ovary	SRR060650 Testis	SRR060651 Ovary	SRR060652 Testis	SRR060653 Ovary	SRR065151 Ovary	SRR065152 Ovary	SRR065153 Ovary	SRR065154 Ovary	SRR065155 Ovary	SRR065800 Ovary	SRR065801 Ovary	SRR065802 Ovary	SRR065803 Ovary	SRR065804 Ovary	SRR065805 Ovary	SRR065806 Ovary	SRR065807 Ovary	SRR097865 S2-NP	SRR097866 S2-NP	SRR097867 S2-NP	SRR298536 Ovary	SRR298537 Ovary	SRR298711 Ovary	SRR298712 Ovary	SRR317110 Ovary	SRR317111 Ovary	SRR317112 Ovary	SRR317113 Ovary	SRR317114 Ovary	SRR317115 Ovary	SRR317116 Ovary	SRR317117 Ovary	SRR341115 S2	SRR341116 S2	SRR341117 S2	SRR341118 S2	SRR351332 Mixed-body	SRR351333 Mixed-body	T004 OSC	T005 CME-L1	V001 Embryo	V002 Embryo	V003 S2	V006 Female-body	V007 Head	V008 S2	V009 CME-L1	V010 ML-DmD20-C5	V011 Sg4	V012 Male-body	V013 Head	V014 DTT	V015 DreRFHV	V018 Kc167	V019 GM2	V020 S2-R+	V021 ML-DmD21	V022 ML-DmD32	V023 Head	V024 Kc167	V025 S2-R+	V026 1182-2H	V027 ML-DmD21	V028 CME-W1-Cl.8+	V029 ML-DmD9	V030 ML-DmD8	V031 GM2	V032 S1	V033 CME-W1-Cl.8+	V034 ML-DmD16-C3	V035 ML-DmD32	V036 ML-DmD20-C5	V037 S2	V038 S2	V063 Ovary	V0632 Ovary	V064 Ovary	V0642 Ovary	V065 Ovary	V0652 Ovary	V066 Ovary	V0662 Ovary	V067 S2-NP	V073 mbn2	V074 S3	V075 ML-DmBG1-C1	V076 ML-DmBG3-C2	V077 Head	V078 Head	V079 Head	V080 Head	V081 Embryo	V082 Embryo	V083 Male-body	V084 Female-body	V085 CME-W2	V086 Female-body	V088 Ovary	V0882 Ovary	V089 Ovary	V0892 Ovary	V090 Male-body	V091 fGS/OSS	V092 Male-body	V093 Male-body	V094 S2-R+	V095 S2-R+	V096 Ovary	V097 Ovary	V098 Ovary	V125 ML-DmD9	V126 CME-L1	V127 G2	V128 S3	V129 ML-DmBG1-C1	V130 ML-DmBG3-C2	V131 ML-DmD16-C3	V132 ML-DmD32	V133 Sg4	V134 ML-DmD8	V135 CME-W2	V136 Male-body	V137 Head	V138 Male-body	V139 Female-body	V140 Female-body	V141 Female-body	V142 Female-body	V144 OSC	V145 S2	V146 S1	V147 1182-2H	V148 mbn2	GSM1528798 follicle cells
...................................................................................................................................................................................................................................................................................................................AAACATTGGC-AAA--------------------------------CATA-TTG-CGT.................................	23	1	6.00	6	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	6	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...............................................................................................................................................................................................................................................CTCC-GCTTTCCTTCGCTGTTTTCGT.....................................................................................................................................	25	1	6.00	6	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	6
.......................................................................................................................................................................................AGGTCACACACCAACACGA--------T---TCGTGCG.................................................................................................................................................................................	27	1	4.00	4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.....................................................................................................................................................................................................................................ATTTATTTTGCTCC-GCTTTCCT..................................................................................................................................................	22	1	4.00	4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0
.................................................................................................................................................................................................................................................................................................ATCCAGGAATCGTGT-TCAAAC.......................................................................................	21	1	4.00	4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
................................................................................................................................................................................................ACCAACACGA--------T---TCGTGCG.................................................................................................................................................................................	18	1	4.00	4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
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..............................................................................................................................................................................AGTGTAGTTAGGTCACACAC............................................................................................................................................................................................................	20	1	3.00	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
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...............................................................................................................................................................................................................................................CTCC-GCTTTCCTTCGCTGTTTTCACAC...................................................................................................................................	27	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...........................................................................................................................................................................................................................................TTTGCTCC-GCTTTCCTTCGCTGT...........................................................................................................................................	23	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.....................................................................................................................................................................................................................................................................................................................ACATTGGC-AAA--------------------------------CATA-TTG-....................................	18	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
........................................................................................................................................................................................................................................................................................AAAAAAC--ATCCAGGAATCGTGT-TCA..........................................................................................	25	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...................................................................................................................................................................................................................................................................TTTCACACCCACGATCCGCGAA.....................................................................................................................	22	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
....................................................................................................................................................................................................................................................GCTTTCCTTCGCTGTTTTTT......................................................................................................................................	20	4	0.25	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

droEre2

T--AAAAAAGACATT-CCAAACACTACGCAACACTCCCCCGCAAA-TACCGCAAAACAGATGTTTCGGTATTTTTTATGTATTGGCACGCAGATTTCAG------------------------------------------------C----------------------ATTTAGTGTAGTTAGGTCACACACCAACACGA--------T---TTGTGCGTAGTTTTCATTTATTTTGCTCC-GCTTTCCTTCGCTGTTTTCACACCCACGATCCGCGAAAAA-AAACACCAAGGAATTGTGT-CTAAACATTGGC-AAA--------------------------------CATA-TTG-CGTAC-----------C-CCCGC--GGGC--ATAA-	Size	Hit Count	Total Norm	Total	M055 Female-body	M057 Embryo	V060 Head	V040 Embryo	V107 Male-body	V108 Head	GSM1528801 follicle cells
................................................................................................................................................................................................ACCAACACGA--------T---TTGTGCG.................................................................................................................................................................................	18	1	2.00	2	0	0	0	0	0	2	0
......................................................................................................................................................................................................................TTGTGCGTAGTTTTCATTTA....................................................................................................................................................................	20	1	1.00	1	0	0	0	0	0	1	0
............................................................................................................................................................................................ACACACCAACACGA--------T---TTGTGCGT................................................................................................................................................................................	23	1	1.00	1	0	0	0	0	1	0	0
..................................................................................................................................................................................................................T---TTGTGCGTAGTTTTCATT......................................................................................................................................................................	19	1	1.00	1	0	0	0	0	0	1	0
.........................................................................................................................................................................................GTCACACACCAACACGA--------T---TTGTGCGTA...............................................................................................................................................................................	27	1	1.00	1	0	0	0	0	0	1	0
....................................................................................................................................................................................................................................................................................................................................................................A-TTG-CGTAC-----------C-CCCGC--GGGC--A....	20	1	1.00	1	1	0	0	0	0	0	0
................................................................................................................................................................................................................................TTTTCATTTATTTTGCTC............................................................................................................................................................	18	2	0.50	1	0	0	0	0	0	1	0

droYak3

T--GAACAAGACATTCC-GCATACTACGCAGCACTCCCCCGCAAA-TACTGCAAAACAGATGTTTCGGTATTTTTTATGTATTGGCACGCAGATTTCAG------------------------------------------------T----------------------GCTTAGTGTAGTTAGGTCACACACCAACACGA--------T---TCGTGCGTAGTTTTCATTTATTTTGCTCC-GCTTTCCTTCGCTGTTTTCACACCCACGATCCGCGAAAAAAAC--AGCCAGGAATTGTGT-CCAAACATTAGC-AAA--------------------------------CATA-TTG-CGTAC-----------C-CCCGC--GGGC--ATAA-	Size	Hit Count	Total Norm	Total	M026 Head	M043 Female-body	M056 Embryo	V046 Embryo	V052 Head	V058 Head	V120 Male-body	SRR1275488 Male larvae	SRR1275486 Male prepupae	SRR1275484 Male prepupae	GSM1528802 follicle cells
..............................................................................................................................................................................................................................................................................................................................AAA--------------------------------CATA-TTG-CGTAC-----------C-CCCGC--GGGC--.....	25	1	3.00	3	0	0	2	0	0	0	1	0	0	0	0
...........................................................................................................................................................................................................................................................................................AAAC--AGCCAGGAATTGTGT-CC...........................................................................................	21	1	2.00	2	0	0	0	0	0	0	2	0	0	0	0
..............................................................................................................................................................................................ACACCAACACGA--------T---TCGTGCG.................................................................................................................................................................................	20	1	2.00	2	0	0	1	1	0	0	0	0	0	0	0
..........................................................................................................................................................................................................................................................CTTCGCTGTTTTCACACCCA................................................................................................................................	20	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0
......................................................................................................................................................................................................................................................................................GAAAAAAAC--AGCCAGGAATTGTGT-.............................................................................................	24	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
............................................................................................................................................................................................................................................TTGCTCC-GCTTTCCTTCGCT.............................................................................................................................................	20	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0
..............................................................................................................................................................................................................................................GCTCC-GCTTTCCTTCGCTGT...........................................................................................................................................	20	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0
.................................................................................................................................................................................................................................................................................................AGCCAGGAATTGTGT-CCAAACATT....................................................................................	24	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
.................................................................................................................................................................................................................................................................GTTTTCACACCCACGATCCGC........................................................................................................................	21	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
.........................................................................................................................................................................................................................................................CCTTCGCTGTTTTCACACCCACG..............................................................................................................................	23	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0
......................................................................................................................................................................................................................................................TTTCCTTCGCTGTTTTCAC.....................................................................................................................................	19	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
...............................................................................................................................................................................................................................................................................................................................AA--------------------------------CATA-TTG-CGTAC-----------C-CCCGC--GGGC--.....	24	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
............................................................................................................................................................................................................................................................................................AAC--AGCCAGGAATTGTGT-C............................................................................................	19	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
.......................................................................................................................................................................................................................................................................................AAAAAAAC--AGCCAGGAATTGTGT-CC...........................................................................................	25	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
.........................................................................................................................................................................................................A--------T---TCGTGCGTAGTTTTCATTT.....................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
...........................................................................................................................................................................................................................................................................................AAAC--AGCCAGGAATTGTGT-.............................................................................................	19	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
............................................................................................................................................................................................ACACACCAACACGA--------T---TCGTGCGT................................................................................................................................................................................	23	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
............................................................................................................................................................................................ACACACCAACACGA--------T---TCGTGCG.................................................................................................................................................................................	22	1	1.00	1	0	0	0	0	0	1	0	0	0	0	0
.........................................................................................................................................................................................GTCACACACCAACACGA--------T---TCGTGCG.................................................................................................................................................................................	25	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0
..........................................................................................................................................................................................TCACACACCAACACGA--------T---TCGTGCG.................................................................................................................................................................................	24	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
.................................................................................................................................................................................................................................................................................................AGCCAGGAATTGTGT-CCAAACATTAGC-A...............................................................................	28	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
...............................................................................................................................................................................................CACCAACACGA--------T---TCGTGCGT................................................................................................................................................................................	20	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
............................................................................................................................................................................................................................................................TCGCTGTTTTCACACCCACG..............................................................................................................................	20	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0
............................................................................................................................................................................................................................................................................................AAC--AGCCAGGAATTGTGT-CCAA.........................................................................................	22	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
...........................................................................................GATTTCAG------------------------------------------------T----------------------GCTTAGTGTAGTT.......................................................................................................................................................................................................................	22	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
.............................................................................................................................................................................................................................................TGCTCC-GCTTTCCTTCGCTGT...........................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
..........................................................................................................................................................................................................................................................................................AAAAC--AGCCAGGAATTGTGT-CC...........................................................................................	22	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0

droEug1

T--AAA-----AAATACC--ATTCGACGCAGGACTGCCCTGCACA-TACCTTAAAACATATGGTTCGGTATTTCTTGTGTATTGGTATGCAGATTCCCGTGTATGTT---AAAATGTC--------TGTTAAAAATAAGTGTGACCGCGCTGGTGCATATTTCTAATAACCTGTGTTGCCGTTTGGTCACA--CGGACCCGA--------A---TCGTGCGTAATTTCCATTTATTTTGCTCC-GCTTTC-TTCGCTGTTTTCACACCCACGATCCGCGAAAAA-A---TCCCAGGAATTG-GT-ACAAACATCGGC-AAA--------------------------------CATA-TTGGTGTTC-----------G-TTGGC--GGGC--ATAA-	Size	Hit Count	Total Norm	Total
No Reads

droBia1

-----------------------------------------CACA-TACCGCAAAACATATGTTTCGGTATTTTTTGTGTATTGGCATTCAGTTTAT---------TACAAAAATATCGAAATATATT---------------------TTGGT--ATATTTTTC-----AC-TACAGTAGTTAGGTCACACCCCACGTATA--------T---TCGTGCGTAATTTTCATTTATTTTGCTCC-GCTTTCCTCCGCTGTTTTCTCACCCACGGTTCGCGAAAAA-----CCCCAGGAATCGTTGGCAAAACAGTGGC-AAA--------------------------------CATA-TTG-TGCGC-----------G-TTCGC--GGAC--ATAA-	Size	Hit Count	Total Norm	Total
No Reads

droTak1

GCCAAA--AGAAAATACC-----------AACACGCCCCCGCACA-TACCGCA-AACATATGTTTCGGTATTTTTTGTGTATTGGCACGCAGAATTCTC------------------------------------------------G----------------------GCTTGTTGTAGTTAGGTCACACACCGAAACAA--------A---TCGTTCGTAATTTTCATTTATTTTGCTCC-GCTTTCCT-CGCTGTTTTCACACCCACGGTTCGCGAAAAA-AC---CCCTGGAATCGTTTGCCAAACATTGGC-AAA--------------------------------CATA-TTG-TGTGC-----------A-TTCGC--GGGC--ATAA-	Size	Hit Count	Total Norm	Total
No Reads

droEle1

C--TAA--GGAAAATACC------AAGTATACACTCC----------ACCGCAAAACATATGTTTCGGTATTTTTTATGTATTGGCATGCAGATTTCCG------------------------------------------------C----------------------AGTTATTGTAGTTAGGTCACACACCGACACAA--------A---TCGTGCGTAATTTTCATTTATTTTGCTCC-GCTTTCCTTCGCTGTTTTCACACCCACAATCGGGAAAAAT-AC--ACCCAGGAGTTGGGA-CCAAACATTGGA-CAA--------------------------------CATA-TTG-TGTAC-----------G-TTCGC--GGGC--ATA--	Size	Hit Count	Total Norm	Total
No Reads

droRho1

CCCAGA--CGAAAATACC------AAGTGTTCACACCCCCGCACA-TACCGCAAAACATATGTTTTGGTATTTTTTGTGTATTGGCATGCATATTTCCG------------------------------------------------C----------------------GCTCAGTGTAGTTAGGTCACACACCGACACAA--------A---TCGTGCGTAATTTTCATTTATTTTGCTCC-GCTTTCCTTCGCTGTTTTCACACCCACTATCGGCAAAAAT-AA--ATCCAGGAATTGGGC-CCAAACATTGGC-CAA--------------------------------CACA-TTG-TGTGC-----------G-TTCGC--GGGC--ATAG-	Size	Hit Count	Total Norm	Total
No Reads

droFic1

C--ACA--AGAACGTACT------TGTC-----C-ACCCTGTACTCTCCCGCA-AACATATGGTTAGGTATTTTTTGTGTATTGGTATGCTGATTTCCG------------------------------------------------C----------------------GCTTAGCGCAGTTTGGTCACACACCGCCACAA--------A---TCGTGCGTAATTTTCATTTATTTTGTTCC-GCTTTCCTTCGCTGTTTTCACACCCACGGTCCGCGAAAAC-AC---TTCAGAAATTGTGC-CCAAACATTGGC-ATA--------------------------------CATA-TTG-AGTGC-----------A-TTTGT--GGTT--ATA--	Size	Hit Count	Total Norm	Total
No Reads

droKik1

----------------------------------------------------------------TTGGTATTTTTTC------AAAAAGAAGGGTTTAT------------------------------------------------C----------------------CCTGGGTTGGGTACGGTCACATTGCAACGAAAACAACAACTCCGTAACTCGTAATTTCGATTTATTTTGC------TTTCATTCGCTATTTTCACACCCACAATCTGCGAAAAT-AA--GGC-------------CATAACCGTGGC-ACG--------------------------------CATA-TTG-TGCGTTCTATCAGTGTG-TTCGCGCGGTCCAACAA-	Size	Hit Count	Total Norm	Total
No Reads

droAna3

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CGTAATTTCCATTTATTTTGCTCT-GCTTTCTTTCGCCGTTTTTCCACCCACAATCCGCAGAAAA-AT--ATCCCGAATTTGTG--CGAAACGGTGGCCCCA--------------------------------CATA-TTG-TGCGTATAATTTCTTAG-TTCAC--GGTG--TTAA-	Size	Hit Count	Total Norm	Total	M044 Female-body	M058 Embryo	V039 Embryo	V055 Head	V105 Male-body	V106 Head
.............................................................................................................................................................................................................................................................................................................................................................................GTATAATTTCTTAG-TTCAC--GGTG--TT...	25	1	2.00	2	0	0	0	0	0	2
...........................................................................................................................................................................................................................................................................................................................................................................GCGTATAATTTCTTAG-TTCAC--GGT........	24	1	1.00	1	0	1	0	0	0	0
.......................................................................................................................................................................................................................................................................................................................GGTGGCCCCA--------------------------------CATA-TTG-TGCGT...............................	22	1	1.00	1	0	0	0	0	1	0
...............................................................................................................................................................................................................................................................................................................................................................................ATAATTTCTTAG-TTCAC--GGTG--AC...	23	1	1.00	1	0	1	0	0	0	0
.......................................................................................................................................................................................................................................................................................................AATTTGTG--CGAAACGGTGGCCCCA--------------------------------CA...........................................	26	1	1.00	1	1	0	0	0	0	0
....................................................................................................................................................................................................................................................................TTTCCACCCACAATCCGCAGA.....................................................................................................................	21	1	1.00	1	0	0	1	0	0	0
............................................................................................................................................................................................................................................................................................................GTG--CGAAACGGTGGCCCCA--------------------------------.............................................	19	1	1.00	1	0	0	0	0	0	1
.........................................................................................................................................................................................................................................................................................AAA-AT--ATCCCGAATTTGTG--CGA..........................................................................................	22	1	1.00	1	0	1	0	0	0	0
.......................................................................................................................................................................................................................................TTATTTTGCTCT-GCTTTCTTT.................................................................................................................................................	21	1	1.00	1	1	0	0	0	0	0

droBip1

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CGTAATTTCCATTTATTTTGCTCT-GCTTTCTTTCGCCGTTTTTCCACCCACAATCCGCAGAAAT-AT--ATCCCGATTTTGTG--CGAAACGGTGGCCCCA--------------------------------CATA-TTG-TGCGTGTAGTTTCTTAG-TTCGC--GATA--ATAAA	Size	Hit Count	Total Norm	Total
No Reads

dp5

----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TCGTGCGTAATTTCCATTTATTTTGCTCCTGCTTTCATCAAATGTTTTCACACACTTGAACTGCATAAAT-AA--TTACAGAAATTGTGT-AAAGACCTTG-CAACGAGTGCACTAATCACGTATTAAATATTTGTCTTGGTGTTCG-CTTTC-----------G-TTTTC--GGGC--ATAA-	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
............................................................................................................................................................................................................................................TTGCTCCTGCTTTCATCAAATGC...........................................................................................................................................	23	1	1.00	1	0	0	1	0	0	0	0	0	0	0
.....................................................................................................................................................................................................................................................................TCACACACTTGAACTGCATAAAT-A................................................................................................................	24	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.................................................................................................................................................................................................................................................................GTTTTCACACACTTGAACTGC........................................................................................................................	21	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.................................................................................................................................................................................................................................................................................................................AAAGACCTTG-CAACGAGTGCACTAATCAC...............................................................	29	1	1.00	1	0	0	0	0	0	0	1	0	0	0
.................................................................................................................................................................................................................................................................GTTTTCACACACTTGAACTGCATAAAT-.................................................................................................................	27	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.................................................................................................................................................................................................................................................................................................................................................................GGTGTTCG-CTTTC-----------G-TTTTC--GGGC--.....	23	1	1.00	1	0	0	0	0	1	0	0	0	0	0
................................................................................................................................................................................................................................................................TGTTTTCACACACTTGAACTGCATAAAT-.................................................................................................................	28	1	1.00	1	0	1	0	0	0	0	0	0	0	0
....................................................................................................................................................................................................................................................................................................................GACCTTG-CAACGAGTGCACT.....................................................................	20	1	1.00	1	0	0	0	0	0	0	1	0	0	0
.................................................................................................................................................................................................................................................................................................................................................................GGTGTTCG-CTTTC-----------G-TTTTC--...........	19	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.......................................................................................................................................................................................................................................................................................TAAAT-AA--TTACAGAAATTGTGT-.............................................................................................	22	1	1.00	1	0	1	0	0	0	0	0	0	0	0

droPer2

----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TCGTGCGTAATTTCCATTTATTTTGCTCCTGCTTTCATCAAATGTTTTCACACACTTGAACTGCATAAAT-AA--TTACAGAAATTGTGT-AAAGACCTTG-CAACGAGTGCACTAATCACGTATTAAATATTTGCCTTGGTGTTCG-CTTTC-----------G-TTTTC--GGGC--ATAA-	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
....................................................................................................................................................................................................................................................................................................................................................................GTTCG-CTTTC-----------G-TTTTC--GGGC--.....	20	1	3.00	3	3	0	0	0	0	0
...............................................................................................................................................................................................................................................................................................................................................GTATTAAATATTTGCCTTGGT..........................................	21	1	1.00	1	0	0	1	0	0	0
........................................................................................................................................................................................................................................................TCATCAAATGTTTTCACACAC.................................................................................................................................	21	1	1.00	1	0	1	0	0	0	0
.................................................................................................................................................................................................................................................................GTTTTCACACACTTGAACTGCATAAAT-A................................................................................................................	28	1	1.00	1	0	1	0	0	0	0
.....................................................................................................................................................................................................................................................................................................................................................................TTCG-CTTTC-----------G-TTTTC--GGGC--.....	19	1	1.00	1	1	0	0	0	0	0
........................................................................................................................................................................................................................................................................................................................................................ATTTGCCTTGGTGTTCG-CTTT................................	21	1	1.00	1	0	0	1	0	0	0

dsi_23971

3l:1479274-1479423 +

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads