dsi_23638

CTTTTGGTAGGAAGCTAACTTTATCATTTCGCAGCAGCAGATTTGCTTTCGTAAGTTTTATAAGATTCTTTTAAAGGTAATACGGGCCCGACTAGAAAGGGTATTCCTTTTTTTTAGTTTCTATTGCGTTTCTATATACAATTTTATACACATTTCTACGATATCAAACAACGACACGAGTGTAGATATTCCGAAAAATTGTATTACCAATTAATTAGGAGTTTATTCTTCGAGTTACTAATTTCTTTCA *******************************************************************...(((.((((((.((....((((((((((((....)))..))))))))))))))))).))).......................((((((...((............)).))))))**************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	SRR553485 Chicharo_3 day-old ovaries	M025 embryo	SRR618934 dsim w501 ovaries	SRR553486 Makindu_3 day-old ovaries	SRR553487 NRT_0-2 hours eggs	M023 head	SRR553488 RT_0-2 hours eggs	M053 female body
.................................................................................................................................................................TATCAAACAACGACACGAGTGTAGA................................................................	25	0	1	4.00	4	4	0	0	0	0	0	0	0
.....................................................................TTTAAAGGTAATACGGGCCCGACTAGA..........................................................................................................................................................	27	0	1	2.00	2	1	0	0	0	1	0	0	0
.....................................................................................................................................................................................GTAGATATTCCGAAAAATTGT................................................	21	0	1	2.00	2	0	2	0	0	0	0	0	0
.........................................................................................................................................................................AACGACACGAGTGTAGATATTCC..........................................................	23	0	1	1.00	1	0	1	0	0	0	0	0	0
.....................................................................................................................................................................................................ATTGTATTCCCAATTAATTAGGA..............................	23	1	1	1.00	1	0	0	0	0	0	0	0	1
....................................................................................................................................................................................TGTAGATATTCCGAAAAATTGTATTACC..........................................	28	0	1	1.00	1	1	0	0	0	0	0	0	0
.........................................................................................................................................................................AACGACACGAGTGTAGATATTCCGAA.......................................................	26	0	1	1.00	1	0	1	0	0	0	0	0	0
.......................................................................................................................................................................................AGATATTCCGAAAAATTGTA...............................................	20	0	1	1.00	1	0	0	1	0	0	0	0	0
..........................................................................................................................................................................................TATTCCGAAAAATTGTATTACCAAT.......................................	25	0	1	1.00	1	1	0	0	0	0	0	0	0
...........................................................................................................................................................................CGACACGAGTGTAGATATTCCGAAA......................................................	25	0	1	1.00	1	0	1	0	0	0	0	0	0
.................................................................................................................................................................................GAGTGTAGATATTCCGAAAAATTG.................................................	24	0	1	1.00	1	1	0	0	0	0	0	0	0
............................................................................................................................................................TACGATATCAAACAACGACACGAGTGTAGA................................................................	30	0	1	1.00	1	1	0	0	0	0	0	0	0
....................................................................TTTTAAAGGTAATACGGGCC..................................................................................................................................................................	20	0	1	1.00	1	0	0	0	0	0	1	0	0
....................................................................................................................................................................................TGTAGATATTCCGAAA......................................................	16	0	1	1.00	1	0	0	0	1	0	0	0	0
................................................................................................................................................................................CGAGTGTAGATATTCCGAAAAATTGTA...............................................	27	0	1	1.00	1	0	1	0	0	0	0	0	0
.........................................................................................................................................................................AACGACACGAGTGTAGATATTCCGAG.......................................................	26	1	1	1.00	1	0	1	0	0	0	0	0	0
......................................................................................................................................TATACAATTTTATACACATTTCTACGA.........................................................................................	27	0	1	1.00	1	1	0	0	0	0	0	0	0
.....................TATCATTTCGCAGCAGCAGATTTGCGTT.........................................................................................................................................................................................................	28	1	1	1.00	1	0	0	0	1	0	0	0	0
.....................................................................TCTAGAGGTAATACGTGCCC.................................................................................................................................................................	20	3	1	1.00	1	0	0	0	1	0	0	0	0
...................................................................................................................................................................TCAAACAACGACACGAGT.....................................................................	18	0	1	1.00	1	0	0	1	0	0	0	0	0
................................................................................TACGGGCCCGACTAGAAAGGGTAT..................................................................................................................................................	24	0	1	1.00	1	0	0	1	0	0	0	0	0
.................................GCAGCAGATTTGCTTTCGTA.....................................................................................................................................................................................................	20	0	1	1.00	1	0	0	1	0	0	0	0	0
.............................................................................................................................................................ACGATATCAAACAACAACAAGAGTGTAGA................................................................	29	2	1	1.00	1	1	0	0	0	0	0	0	0
...................................................................................................................................................................TCAAACAACGACACGAGTGTAGATATT............................................................	27	0	1	1.00	1	1	0	0	0	0	0	0	0
....................................................................................................................................................................................TGTAGATATTCCAAAAAATTGTATTA............................................	26	1	1	1.00	1	1	0	0	0	0	0	0	0
............................................................................................................................................................TACGATATCAAACAACGACACGAGT.....................................................................	25	0	1	1.00	1	0	0	1	0	0	0	0	0
.................ACTTTATCATTTCGCAGCAGCAGA.................................................................................................................................................................................................................	24	0	1	1.00	1	1	0	0	0	0	0	0	0
.........................................TTTGCTTTCGTAAGTTTTATAAGATTCT.....................................................................................................................................................................................	28	0	1	1.00	1	1	0	0	0	0	0	0	0
.....................................................................................................................................................................AAACAACGACACGAGTGT...................................................................	18	0	1	1.00	1	0	0	1	0	0	0	0	0
.........................................................GTATGAGATTCTTTTAAA...............................................................................................................................................................................	18	2	17	0.47	8	0	0	0	0	4	0	4	0
..........................................................TATGAGATTCTTTTAAA...............................................................................................................................................................................	17	1	3	0.33	1	0	0	0	0	0	0	1	0
..........................................................................................................................................................................................AATTCCGAAAAATTGTATT.............................................	19	1	3	0.33	1	1	0	0	0	0	0	0	0
...........................................................................................................................................................................................ATTCCGGAGAATTGTATT.............................................	18	2	4	0.25	1	0	0	1	0	0	0	0	0
.......................................................................TAGAGCTAATACGTGCCC.................................................................................................................................................................	18	3	20	0.20	4	0	0	0	4	0	0	0	0
....................................................................................................................................................................................TGTAGATATTCGGAAGAA....................................................	18	2	6	0.17	1	0	0	0	0	0	0	1	0
............................................................................................................TTTTTTTAGTTTCTA...............................................................................................................................	15	0	6	0.17	1	0	0	0	0	0	0	1	0
......................................................................TTAGAGGTACGACGGGCC..................................................................................................................................................................	18	3	13	0.15	2	0	0	0	0	0	2	0	0
........................................................................................................................................................................................................................AGGTGTTTATTGTTCGA.................	17	2	20	0.10	2	0	0	0	2	0	0	0	0
.....................................................................TTTAAAGGTAGTACGGG....................................................................................................................................................................	17	1	11	0.09	1	0	0	1	0	0	0	0	0
.................................................................................ACGGGCCCGGCTAGC..........................................................................................................................................................	15	2	20	0.05	1	0	0	1	0	0	0	0	0
....................................................................................................................................................................................TGTAGATATTCGGAAACAC...................................................	19	3	20	0.05	1	0	0	0	0	1	0	0	0
....................................................................................................................................................................................TGTAGATATTCGGAAGA.....................................................	17	2	20	0.05	1	0	0	0	0	0	0	1	0
....................................................................................................................................................................................TGTAGATATTCGGAATAAC...................................................	19	3	20	0.05	1	0	0	0	0	0	0	1	0

GAAAACCATCCTTCGATTGAAATAGTAAAGCGTCGTCGTCTAAACGAAAGCATTCAAAATATTCTAAGAAAATTTCCATTATGCCCGGGCTGATCTTTCCCATAAGGAAAAAAAATCAAAGATAACGCAAAGATATATGTTAAAATATGTGTAAAGATGCTATAGTTTGTTGCTGTGCTCACATCTATAAGGCTTTTTAACATAATGGTTAATTAATCCTCAAATAAGAAGCTCAATGATTAAAGAAAGT **************************************************************...(((.((((((.((....((((((((((((....)))..))))))))))))))))).))).......................((((((...((............)).))))))*******************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	SRR902009 testis	GSM343915 embryo	SRR553488 RT_0-2 hours eggs	SRR553487 NRT_0-2 hours eggs	SRR618934 dsim w501 ovaries
........................................................................................................................................................AAAGATGCTATAGTTTGTTGC.............................................................................	21	0	1	1.00	1	1	0	0	0	0
......................................................................................................................ACGATAACGTAAGGATATATG...............................................................................................................	21	3	1	1.00	1	0	1	0	0	0
.....................................................................................................................................................TGTGAAGATGCTATGGT....................................................................................	17	2	11	0.09	1	0	0	1	0	0
...................................................................................................................................................................................................................................GATGCTCTATGATTTAAGA....	19	3	19	0.05	1	0	0	0	1	0
.GAAACCATGCTTCGAATG.......................................................................................................................................................................................................................................	18	3	20	0.05	1	0	0	0	1	0
..........................................................................................................................................................................................ATAAGGCTTTCTAGCA................................................	16	2	20	0.05	1	0	0	0	0	1
........................................................................................................................................................................................ATATAAGGCTTTTTAA..................................................	16	1	20	0.05	1	0	0	1	0	0

Species	Coordinate	ID	Alignment
droSim2	3l:22531127-22531376 -	dsi_23638	CTTT--TGGTAG------GAAGCTA---------------------------------AC------TTTATCATTTCGCAGCAGCAGATTTGCTTTCGTAAGTTTT------ATAAGA-----------------------TTCTTTTA--------------------------------------AAGGTAATACGGGCCCGACTAGAAAGGGTATTCCTTTTTTTTAGTTTCTATTGCGTTTCTATATAC------------AATTTTATACACATTTCTACGATATCAAACAACGACACGAGTGTAGAT----------AT-TCCGAAAAATTGTATT------------------------------------------------------------------------ACCAATTAATTAGGAGTTTATTCT--TCGAGTTACTAATTTCTTTCA----
droSec2	scaffold_41:67594-67876 -		CTTTT-TGGTAG------GAAGCTAAGAATGCCTTTAAAATTATTATGTATTGTTTTAAC------TTTATCATTTCGCAGCAGCAGATTTGCTTTCGTAAGTTTT------AAAAGA-----------------------TTCTTTTA--------------------------------------TAGGTAATACGGGCCCGACAAGAAAGGGTATTCCTT-TTCTTAGTTTCTATTGCGTTTCTATATAC------------AATTTTATACACATTTCTACGATATCAAACAACAACACGAGTGTAGAT----------AT-TCCGAAAAATTGTATT------------------------------------------------------------------------ACCAATTAATTAGGAGTTTATTCT--TCGAGTTACTAATTTCTTTCA----
dm3	chr3L:23086383-23086720 -		TTTT--TGGTAG------GAAGCTCAGAATGCCCTT----------TGTATTGTTTTAAC------TTTATTATTTCGCAGCAGCAGATTTGCTTTCGTAGAAAGAAAAAAAGAAAGA-----------------------TTGTTTTAAGCGTGTAGAAAAGGTTAATTAGATTAAAAGCAAATTGAAGGTAATACGGGTCCGACTAGAATGGGTACTCCTT-T-TCTCGTTTTTATTGCGTTTCTATATACATAATTATATACAATTTTATACATCTTTTTACAATCTCAAGCAACAACACGATTGTAGGTATTCTCTGTCGATTCGGAAACATTTTATT------------------------------------------------------------------------ACCCAGTAATTTGGAATTTATTTT--TTGAGTCTCTAATTTCTTTGG----
droEre2	scaffold_4784:22725533-22725910 +		TTTG--TGGATT------TAAGCTCAGTATGCTCTTTAAATT----TGTTTTGTTCTAATAAGTGGTTTATAATTTCACAGCAGCAGATCAGCCTTCGTTAACTGA------GGTCGACATGCCATGTAAAATACAAAAGATTC----------------------------------------------------------GGAATACCCTCGGGACTTTGATTTCTGTGTGGCTATTGAGCTGATTTATATGTATATGTACATCAGGTGAT-TGTCTTTCTACGAAATCAAACAGTATCATAACA-----TTCTAGCAGTTAA-TCCGGAAAAATGTCTTGTTAAATATGAAATACGTGAGCAAAGAAAATTTGTATTATCTATGTAATATAATTGGTAGTCGTCCTAGTCAACCTATCGCTAAGAAATACAGTCTTATTGCGTCGCTAATTGCCCGAA----
droYak3	3L:23689314-23689414 -		TTTA--TGGTACTATTACTATGTTCAGTATGATCATTAAATT----AGTTTTGTTCCAGT------T---TTTTTTCGCAACAGCAGATCAACCTTCGTTGATTGT------GGTAGA-----------------------TTTT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droEug1	scf7180000406959:25858-25890 +		TTGA--TGGTAC------ACAACTT----------TAAAG------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTTTTTATCAG
droEle1	scf7180000490161:31249-31283 -		TTCTTACGGTAC------ACAG------ATGTTCTTAAAG------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTTCCCA----
droPer2	scaffold_122:62718-62759 +		TTCT---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GAACACATTTCTACCATGGTAAATAAGGACATGGGCGT------------------------------------------------------------------------------------------------------------------------------------------------------------

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droSim2

CTTT--TGGTAG------GAAGCTA---------------------------------AC------TTTATCATTTCGCAGCAGCAGATTTGCTTTCGTAAGTTTT------ATAAGA-----------------------TTCTTTTA--------------------------------------AAGGTAATACGGGCCCGACTAGAAAGGGTATTCCTTTTTTTTAGTTTCTATTGCGTTTCTATATAC------------AATTTTATACACATTTCTACGATATCAAACAACGACACGAGTGTAGAT----------AT-TCCGAAAAATTGTATT------------------------------------------------------------------------ACCAATTAATTAGGAGTTTATTCT--TCGAGTTACTAATTTCTTTCA----	Size	Hit Count	Total Norm	Total	M023 Head	M024 Male-body	M025 Embryo	M053 Female-body	O001 Testis	O002 Head	SRR553485 Ovary	SRR553486 Ovary	SRR553487 Ovary	SRR553488 Ovary	SRR618934 Ovary	V044 Embryo	SRR902008 Ovary	SRR902009 Testis	SRR1275487 Male larvae	SRR1275485 Male prepupae	SRR1275483 Male prepupae
...............................................................................................................................................................................................................................................................................................TATCAAACAACGACACGAGTGTAGA.........................................................................................................................................................	25	1	4.00	4	0	0	0	0	0	0	4	0	0	0	0	0	0	0	0	0	0
.................................................................................................................................................TTTA--------------------------------------AAGGTAATACGGGCCCGACTAGA...............................................................................................................................................................................................................................................................	27	1	2.00	2	0	0	0	0	0	0	1	0	1	0	0	0	0	0	0	0	0
...................................................................................................................................................................................................................................................................................................................GTAGAT----------AT-TCCGAAAAATTGT..............................................................................................................................	21	1	2.00	2	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.......................................................................................................................................................................................................................................................................................................AACGACACGAGTGTAGAT----------AT-TCC........................................................................................................................................	23	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..................................................................................................................................................................................................................................................................................................................TGTAGAT----------AT-TCCGAAAAATTGTATT------------------------------------------------------------------------ACC................................................	28	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0
.......................................................................................................................................................................................................................................................................................................AACGACACGAGTGTAGAT----------AT-TCCGAA.....................................................................................................................................	26	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.....................................................................................................................................................................................................................................................................................................................AGAT----------AT-TCCGAAAAATTGTA.............................................................................................................................	20	1	1.00	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
........................................................................................................................................................................................................................................................................................................................T----------AT-TCCGAAAAATTGTATT------------------------------------------------------------------------ACCAAT.............................................	25	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0
.........................................................................................................................................................................................................................................................................................................CGACACGAGTGTAGAT----------AT-TCCGAAA....................................................................................................................................	25	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...............................................................................................................................................................................................................................................................................................................GAGTGTAGAT----------AT-TCCGAAAAATTG...............................................................................................................................	24	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0
..........................................................................................................................................................................................................................................................................................TACGATATCAAACAACGACACGAGTGTAGA.........................................................................................................................................................	30	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0
................................................................................................................................................TTTTA--------------------------------------AAGGTAATACGGGCC.......................................................................................................................................................................................................................................................................	20	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..................................................................................................................................................................................................................................................................................................................TGTAGAT----------AT-TCCGAAA....................................................................................................................................	16	1	1.00	1	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0
..............................................................................................................................................................................................................................................................................................................CGAGTGTAGAT----------AT-TCCGAAAAATTGTA.............................................................................................................................	27	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.......................................................................................................................................................................................................................................................................................................AACGACACGAGTGTAGAT----------AT-TCCGAG.....................................................................................................................................	26	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0
........................................................................................................................................................................................................................................................TATAC------------AATTTTATACACATTTCTACGA..................................................................................................................................................................................	27	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0
.................................................................................................................................................................................................................................................................................................TCAAACAACGACACGAGT..............................................................................................................................................................	18	1	1.00	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
..................................................................................................................................................................................................TACGGGCCCGACTAGAAAGGGTAT.......................................................................................................................................................................................................................................................	24	1	1.00	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
................................................................................GCAGCAGATTTGCTTTCGTA.............................................................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
.................................................................................................................................................................................................................................................................................................TCAAACAACGACACGAGTGTAGAT----------AT-T..........................................................................................................................................	27	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0
..........................................................................................................................................................................................................................................................................................TACGATATCAAACAACGACACGAGT..............................................................................................................................................................	25	1	1.00	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
..........................................................AC------TTTATCATTTCGCAGCAGCAGA.........................................................................................................................................................................................................................................................................................................................................................................................	24	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0
........................................................................................TTTGCTTTCGTAAGTTTT------ATAAGA-----------------------TTCT................................................................................................................................................................................................................................................................................................................................	28	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0
...................................................................................................................................................................................................................................................................................................AAACAACGACACGAGTGT............................................................................................................................................................	18	1	1.00	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
..............................................................................................................................................................................................................................TTTTTTTAGTTTCTA....................................................................................................................................................................................................................................	15	6	0.17	1	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0

droSec2

CTTTT-TGGTAG------GAAGCTAAGAATGCCTTTAAAATTATTATGTATTGTTTTAAC------TTTATCATTTCGCAGCAGCAGATTTGCTTTCGTAAGTTTT------AAAAGA-----------------------TTCTTTTA--------------------------------------TAGGTAATACGGGCCCGACAAGAAAGGGTATTCCTT-TTCTTAGTTTCTATTGCGTTTCTATATAC------------AATTTTATACACATTTCTACGATATCAAACAACAACACGAGTGTAGAT----------AT-TCCGAAAAATTGTATT------------------------------------------------------------------------ACCAATTAATTAGGAGTTTATTCT--TCGAGTTACTAATTTCTTTCA----	Size	Hit Count	Total Norm	Total	M054 Female-body	V113 Male-body	V114 Embryo	V115 Head
.....................................................................................AGATTTGCTTTCGTAAGT..........................................................................................................................................................................................................................................................................................................................................................................	18	1	2.00	2	0	0	2	0
.................................................................................................................................................................................................................................................................................................................................................................................................................................AATTAATTAGGAGTTTATT.............................	19	1	1.00	1	0	0	1	0
....................................................................................................................................................A--------------------------------------TAGGTAATACGGGCCCGACAAGAAAG............................................................................................................................................................................................................................................................	27	1	1.00	1	0	0	1	0
................................................................................................................................................................................................................................................................................................ATCAAACAACAACACGAGTGTAG..........................................................................................................................................................	23	1	1.00	1	0	0	1	0
....T-TGGTAG------GAAGCTAAGAATGCC................................................................................................................................................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	1	0
...............................................................................................................................................................................................................................................................................................TATCAAACAACAACACGAGTGTAG..........................................................................................................................................................	24	1	1.00	1	1	0	0	0
...................................................................TTATCATTTCGCAGCAGC............................................................................................................................................................................................................................................................................................................................................................................................	18	1	1.00	1	0	0	1	0

dm3

TTTT--TGGTAG------GAAGCTCAGAATGCCCTT----------TGTATTGTTTTAAC------TTTATTATTTCGCAGCAGCAGATTTGCTTTCGTAGAAAGAAAAAAAGAAAGA-----------------------TTGTTTTAAGCGTGTAGAAAAGGTTAATTAGATTAAAAGCAAATTGAAGGTAATACGGGTCCGACTAGAATGGGTACTCCTT-T-TCTCGTTTTTATTGCGTTTCTATATACATAATTATATACAATTTTATACATCTTTTTACAATCTCAAGCAACAACACGATTGTAGGTATTCTCTGTCGATTCGGAAACATTTTATT------------------------------------------------------------------------ACCCAGTAATTTGGAATTTATTTT--TTGAGTCTCTAATTTCTTTGG----	Size	Hit Count	Total Norm	Total	GSM154618 Ovary	GSM154620 Ovary	GSM154621 Ovary	GSM154622 Ovary	GSM180328 Head	GSM180329 Mixed-body	GSM180330 Embryo	GSM180331 Embryo	GSM180332 Embryo	GSM180333 Embryo	GSM180334 Larvae	GSM180335 Disc	GSM180336 Pupae	GSM180337 S2	GSM231091 Ovary	GSM239041 Head	GSM239050 Head	GSM239051 S2	GSM239052 S2	GSM246084 Head	GSM266765 S2	GSM280082 Ovary	GSM280083 Ovary	GSM280084 Ovary	GSM280085 Testis	GSM280086 Ovary	GSM280087 S2-NP	GSM280088 S2-NP	GSM312995 Head	GSM313160 Head	GSM313161 Head	GSM313162 Head	GSM313163 Head	GSM313164 Head	GSM313165 Head	GSM343287 Embryo	GSM379050 Ovary	GSM379051 Ovary	GSM379052 Ovary	GSM379053 Ovary	GSM379054 Ovary	GSM379055 Ovary	GSM379056 Ovary	GSM379057 Ovary	GSM379058 Ovary	GSM379059 Ovary	GSM379060 Ovary	GSM379061 Ovary	GSM379062 Ovary	GSM379063 Ovary	GSM379064 Ovary	GSM379065 Ovary	GSM379066 Ovary	GSM379067 Ovary	GSM424739 S2	GSM424740 S2	GSM424741 S2	GSM467729 Female-body	GSM467730 Female-body	GSM467731 Female-body	M002 CS	M003 Male-body	M004 Male-body	M005 ML-DmD32	M006 ML-DmD32	M029 OSC	M030 OSC	M031 OSC	M032 S2-R+	M033 S2-R+	M034 S2-R+	M035 S2-R+	M036 S2-R+	M037 S2-R+	M038 S2-R+	M039 S2-R+	M048 S2-R+	OSS2 OSS	OSS6 OSS	OSS7 OSS	OSS8 OSS	S1 Head	S10 Embryo	S11 Embryo	S12 Embryo	S13 Embryo	S14 Embryo	S15 S2-GMR	S16 Kc	S17 Larvae	S18 Larvae	S19 Pupae	S2 Head	S20 Pupae	S21 Disc	S22 Head	S23 Female-body	S24 Male-body	S25 Larvae	S26 Larvae	S27 Pupae	S28 Pupae	S29 Head	S3 Male-body	S30 S2-GMR	S31 S2	S32 S2	S33 Kc	S34 Kc	S35 Kc	S4 Female-body	S5 Disc	S6 Embryo	S7 Embryo	S8 S2	S9 Embryo	SRR001337 Head	SRR001338 Head	SRR001339 Head	SRR001340 Head	SRR001341 Head	SRR001342 Head	SRR001343 Head	SRR001344 Head	SRR001345 Head	SRR001346 Head	SRR001347 Head	SRR001348 Head	SRR001349 Head	SRR001664 Head	SRR010951 Ovary	SRR010952 Ovary	SRR010953 Ovary	SRR010954 Ovary	SRR010955 Ovary	SRR010956 Ovary	SRR010957 Ovary	SRR010958 Ovary	SRR010959 Ovary	SRR010960 Ovary	SRR014268 Embryo	SRR014269 Embryo	SRR014270 Embryo	SRR014271 Embryo	SRR014272 Embryo	SRR014273 Ovary	SRR014274 Ovary	SRR014275 Ovary	SRR014276 Ovary	SRR014277 Ovary	SRR014278 Ovary	SRR014279 Ovary	SRR014280 Ovary	SRR014281 Ovary	SRR014282 Ovary	SRR023197 S2	SRR023399 S2	SRR023400 S2	SRR023402 S2	SRR023407 S2	SRR029028 S2	SRR029029 S2	SRR029030 S2	SRR029031 S2	SRR029032 S2	SRR029033 S2	SRR029608 Head	SRR029633 Head	SRR030145 gb3	SRR031692 Head	SRR031693 Head	SRR031694 Head	SRR031696 Head	SRR031697 Head	SRR031698 Head	SRR031699 Head	SRR031700 Head	SRR031701 Head	SRR031702 Head	SRR031703 Head	SRR031704 Head	SRR032092 S2	SRR032093 S2	SRR032094 S2	SRR032095 S2-NP	SRR032096 S2-NP	SRR032152 Head	SRR060643 Testis	SRR060644 Ovary	SRR060645 Testis	SRR060646 Ovary	SRR060647 Ovary	SRR060648 Ovary	SRR060649 Ovary	SRR060650 Testis	SRR060651 Ovary	SRR060652 Testis	SRR060653 Ovary	SRR065151 Ovary	SRR065152 Ovary	SRR065153 Ovary	SRR065154 Ovary	SRR065155 Ovary	SRR065800 Ovary	SRR065801 Ovary	SRR065802 Ovary	SRR065803 Ovary	SRR065804 Ovary	SRR065805 Ovary	SRR065806 Ovary	SRR065807 Ovary	SRR097865 S2-NP	SRR097866 S2-NP	SRR097867 S2-NP	SRR298536 Ovary	SRR298537 Ovary	SRR298711 Ovary	SRR298712 Ovary	SRR317110 Ovary	SRR317111 Ovary	SRR317112 Ovary	SRR317113 Ovary	SRR317114 Ovary	SRR317115 Ovary	SRR317116 Ovary	SRR317117 Ovary	SRR341115 S2	SRR341116 S2	SRR341117 S2	SRR341118 S2	SRR351332 Mixed-body	SRR351333 Mixed-body	T004 OSC	T005 CME-L1	V001 Embryo	V002 Embryo	V003 S2	V006 Female-body	V007 Head	V008 S2	V009 CME-L1	V010 ML-DmD20-C5	V011 Sg4	V012 Male-body	V013 Head	V014 DTT	V015 DreRFHV	V018 Kc167	V019 GM2	V020 S2-R+	V021 ML-DmD21	V022 ML-DmD32	V023 Head	V024 Kc167	V025 S2-R+	V026 1182-2H	V027 ML-DmD21	V028 CME-W1-Cl.8+	V029 ML-DmD9	V030 ML-DmD8	V031 GM2	V032 S1	V033 CME-W1-Cl.8+	V034 ML-DmD16-C3	V035 ML-DmD32	V036 ML-DmD20-C5	V037 S2	V038 S2	V063 Ovary	V0632 Ovary	V064 Ovary	V0642 Ovary	V065 Ovary	V0652 Ovary	V066 Ovary	V0662 Ovary	V067 S2-NP	V073 mbn2	V074 S3	V075 ML-DmBG1-C1	V076 ML-DmBG3-C2	V077 Head	V078 Head	V079 Head	V080 Head	V081 Embryo	V082 Embryo	V083 Male-body	V084 Female-body	V085 CME-W2	V086 Female-body	V088 Ovary	V0882 Ovary	V089 Ovary	V0892 Ovary	V090 Male-body	V091 fGS/OSS	V092 Male-body	V093 Male-body	V094 S2-R+	V095 S2-R+	V096 Ovary	V097 Ovary	V098 Ovary	V125 ML-DmD9	V126 CME-L1	V127 G2	V128 S3	V129 ML-DmBG1-C1	V130 ML-DmBG3-C2	V131 ML-DmD16-C3	V132 ML-DmD32	V133 Sg4	V134 ML-DmD8	V135 CME-W2	V136 Male-body	V137 Head	V138 Male-body	V139 Female-body	V140 Female-body	V141 Female-body	V142 Female-body	V144 OSC	V145 S2	V146 S1	V147 1182-2H	V148 mbn2	GSM1528798 follicle cells
..................................................................................................................................................................................................................................................................................................................TGTAGGTATTCTCTGTCGATTCGGA......................................................................................................................................	25	1	74.00	74	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	2	2	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	1	0	0	0	0	0	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	2	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	2	0	0	0	4	0	10	1	8	0	5	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	2	0	0	0	0	2	0	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	18	0	0	0	0	0
..TT--TGGTAG------GAAGCTCAGAATGCCCTT----------...................................................................................................................................................................................................................................................................................................................................................................................................................................	26	1	36.00	36	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	4	1	0	1	0	0	0	0	0	0	0	0	1	0	0	3	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	3	0	0	0	0	0	0	1	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	17	0	0	0	0	0
.................................................................................................................................................................................................................................................................................................................TTGTAGGTATTCTCTGTCGATTCGGA......................................................................................................................................	26	1	36.00	36	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	1	0	2	0	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	21	0	0	0	0	0
..................................................................................................................................................................................................TACGGGTCCGACTAGAATGGGTAC.......................................................................................................................................................................................................................................................	24	1	16.00	16	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	7	0	4	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..................................................................................................................................................................................................TACGGGTCCGACTAGAATGGGTACT......................................................................................................................................................................................................................................................	25	1	16.00	16	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	1	0	0	0	1	0	3	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0
..............................................................................................................................................TGTTTTAAGCGTGTAGAAAAGGTTAAT........................................................................................................................................................................................................................................................................................................	27	1	15.00	15	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	4	1	4	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0
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.......................................................................TATTTCGCAGCAGCAGATTTGCTTT.................................................................................................................................................................................................................................................................................................................................................................................	25	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
......................................................................................................................................................................................................................................................................................................................................TCGGAAACATTTTATT------------------------------------------------------------------------ACC................................................	19	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...................................................................................................................................................................................................ACGGGTCCGACTAGAATGGGTACTCCTT-.................................................................................................................................................................................................................................................	28	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
....................................................................................................................................................................................................................................................................TATACAATTTTATACATCTTTT.......................................................................................................................................................................................	22	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
........................................................................................................................................................................................................................................................................................................................TATTCTCTGTCGATTCGGAAA....................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
....................................................................................................................................................................................................................................TCGTTTTTATTGCGTTTCTATATACA...................................................................................................................................................................................................................	26	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
............................................................................................................................................................................................................................................................................................................CACGATTGTAGGTATTCTCTGTCGA............................................................................................................................................	25	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

droEre2

TTTG--TGGATT------TAAGCTCAGTATGCTCTTTAAATT----TGTTTTGTTCTAATAAGTGGTTTATAATTTCACAGCAGCAGATCAGCCTTCGTTAACTGA------GGTCGACATGCCATGTAAAATACAAAAGATTC----------------------------------------------------------GGAATACCCTCGGGACTTTGATTTCTGTGTGGCTATTGAGCTGATTTATATGTATATGTACATCAGGTGAT-TGTCTTTCTACGAAATCAAACAGTATCATAACA-----TTCTAGCAGTTAA-TCCGGAAAAATGTCTTGTTAAATATGAAATACGTGAGCAAAGAAAATTTGTATTATCTATGTAATATAATTGGTAGTCGTCCTAGTCAACCTATCGCTAAGAAATACAGTCTTATTGCGTCGCTAATTGCCCGAA----	Size	Hit Count	Total Norm	Total	M055 Female-body	M057 Embryo	V060 Head	V040 Embryo	V107 Male-body	V108 Head	GSM1528801 follicle cells
..TG--TGGATT------TAAGCTCAGTATG..................................................................................................................................................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	1	0	0	0	0	0	0
.....................................................................................AGATCAGCCTTCGTTAACTGA------GGTC.............................................................................................................................................................................................................................................................................................................................................................	25	1	1.00	1	0	0	0	0	0	1	0
...................................................................................GCAGATCAGCCTTCGTTAACTGA------GGTC.............................................................................................................................................................................................................................................................................................................................................................	27	1	1.00	1	0	0	0	0	1	0	0
......................................................................................................................................................................................................................................................................................................................................................................................................................CCTAGTCAACCTATCGCTAAGA.....................................	22	1	1.00	1	1	0	0	0	0	0	0
...................................................................................................................................ATACAAAAGATTC----------------------------------------------------------GGAATACCCTCGGGA........................................................................................................................................................................................................................................................	28	1	1.00	1	0	0	0	0	0	1	0
......TGGATT------TAAGCTCAGTATGCTCTT.............................................................................................................................................................................................................................................................................................................................................................................................................................................	24	1	1.00	1	1	0	0	0	0	0	0
...............................................................................................................................................................................................................................................................ATATGTACATCAGGTGAT-TGT............................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	1	0
..................................................................................................................................................................................................................................................................................................................A-----TTCTAGCAGTTAA-TCCGGAA....................................................................................................................................	21	1	1.00	1	0	0	0	0	1	0	0
............................................................................................................................................................................................................................TGATTTCTGTGTGGCTATTGAGCTGATT.........................................................................................................................................................................................................................	28	1	1.00	1	1	0	0	0	0	0	0
.........................................................................................................................................................................................................................CTTTGATTTCTGTGTGGCTATT..................................................................................................................................................................................................................................	22	1	1.00	1	0	1	0	0	0	0	0
...............................................................................................................................................................................................................................................................................................................................................................................................................................................ACAGTCTTATTGCGTCGCT...............	19	1	1.00	1	0	0	0	1	0	0	0
....................................................................................................................................TACAAAAGATTC----------------------------------------------------------GGAATACCCTCGGGAC.......................................................................................................................................................................................................................................................	28	1	1.00	1	1	0	0	0	0	0	0
...G--TGGATT------TAAGCTCAGTATGCT................................................................................................................................................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	0	0	1	0	0
.........................................................................................................................................................................................................................................................................................................TATCATAACA-----TTCTAGCAGTTAA-TCCGGA.....................................................................................................................................	29	1	1.00	1	1	0	0	0	0	0	0
...................................................................................................................................................................................................................................................................................................................................................................TACGTGAGCAAAGAAAATTTGTATTATCT.................................................................................	29	1	1.00	1	0	0	0	0	0	0	1
................................................................................................................................................................................................................................TTCTGTGTGGCTATTGAGCTGATT.........................................................................................................................................................................................................................	24	1	1.00	1	0	0	0	0	1	0	0
...G--TGGATT------TAAGCTCAGTATGCC................................................................................................................................................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	1	0	0	0	0	0
......................................................................................................................................................................................................................................................................CATCAGGTGAT-TGTCTTTCTACGAAA................................................................................................................................................................................	26	1	1.00	1	1	0	0	0	0	0	0
.TTG--TGGATT------TAAGCTCAGTATGCT................................................................................................................................................................................................................................................................................................................................................................................................................................................	24	1	1.00	1	0	0	0	1	0	0	0
...................................................................................................TAACTGA------GGTCGACATGCCAT...................................................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	1	0	0
..............................................................................................................................................................................................................................................................................................................................................................................................................................................TACAGTCTTATTGCGTCGCTAATTGCC........	27	1	1.00	1	0	0	0	0	1	0	0
......................CTCAGTATGCTCTTTAAAAA----...................................................................................................................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	0	0	0	0	1	0
...........................................................................................................................CATGTAAAATACAAAAGATTC----------------------------------------------------------GGAA...................................................................................................................................................................................................................................................................	25	1	1.00	1	1	0	0	0	0	0	0
...................................................................................................................................................................................................................................TGTGTGGCTATTGAGCTGATTTATA.....................................................................................................................................................................................................................	25	1	1.00	1	1	0	0	0	0	0	0
..TG--TGGATT------TAAGCTCAGTATGCT................................................................................................................................................................................................................................................................................................................................................................................................................................................	23	1	1.00	1	0	0	0	0	0	1	0
.................................................................................................GTTAACTGA------GGTCGACATGCC.....................................................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	1	0	0

droYak3

TTTA--TGGTACTATTACTATGTTCAGTATGATCATTAAATT----AGTTTTGTTCCAGT------T---TTTTTTCGCAACAGCAGATCAACCTTCGTTGATTGT------GGTAGA-----------------------TTTT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M026 Head	M043 Female-body	M056 Embryo	V046 Embryo	V052 Head	V058 Head	V120 Male-body	SRR1275488 Male larvae	SRR1275486 Male prepupae	SRR1275484 Male prepupae	GSM1528802 follicle cells
....................................................GTTCCAGT------T---TTTTTTCGCAACAGCAGA.........................................................................................................................................................................................................................................................................................................................................................................................	27	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0

droEug1

TTGA--TGGTAC------ACAACTT----------TAAAG------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTTTTTATCAG	Size	Hit Count	Total Norm	Total
No Reads

droEle1

TTCTTACGGTAC------ACAG------ATGTTCTTAAAG------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTTCCCA----	Size	Hit Count	Total Norm	Total
No Reads

droPer2

TTCT---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GAACACATTTCTACCATGGTAAATAAGGACATGGGCGT------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
.TCT---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GAACACATTTCTACCATGGT..............................................................................................................................................................................	23	20	1.20	24	15	9	0	0	0	0
.TCT---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GAACACATTTCTACCATGGTA.............................................................................................................................................................................	24	20	0.65	13	0	13	0	0	0	0
.TCT---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GAACACATTTCTACCATGG...............................................................................................................................................................................	22	20	0.35	7	1	6	0	0	0	0
TTCT---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GAACACATTTCTACCAT.................................................................................................................................................................................	21	20	0.05	1	0	1	0	0	0	0
..CT---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GAACACATTTCTACCATGGT..............................................................................................................................................................................	22	20	0.05	1	1	0	0	0	0	0
TTCT---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GAACACATTTCTACCATGGT..............................................................................................................................................................................	24	20	0.05	1	0	1	0	0	0	0
.TCT---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GAACACATTTCTACCAT.................................................................................................................................................................................	20	20	0.05	1	0	1	0	0	0	0
TTCT---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GAACACATTTCTACCATGG...............................................................................................................................................................................	23	20	0.05	1	0	1	0	0	0	0

dsi_23638

3l:22531177-22531326 -

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-16.3	-16.0	-16.0