dsi_20377

CDS [3l_22392864_22393171_+]; exon [3l_22392772_22393171_+]; intron [3l_22393172_22394315_+]

##################################################-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- GAGTACTACGATGCCATGGCCTTCCTCAAGGAGGTCATTCGAGAACGCAAGTAAGACGACGTTTGACCTGCCCCAAGATACTAGCTTATGAGATAGATACCGTTTAAGGTTAGAAGAGCTCAGAGTATAGCTAGGTTGCATTTCTCTAATGTCAATTTTGGACATTCCTGTTATTTGGCAAAGTCGTGTTTGTGGTTTTTGGAACATTTCGCGATATTTATTTCTTTTTATTTCTGTACCCGTTACTATT ************************************************..(((((.(((..((((.(((((((((((.((((((..((((......(((......)))........))))......))))))...(((((.....)))))..)))))))(((....))).....))))..))))....))).))))).************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	O002 Head	M025 embryo	GSM343915 embryo	SRR553488 RT_0-2 hours eggs	M024 male body	SRR553485 Chicharo_3 day-old ovaries	SRR553486 Makindu_3 day-old ovaries	SRR553487 NRT_0-2 hours eggs
...................................................................................................................................................................................................................CGATATTTATTTCTTTTT.....................	18	0	1	2.00	2	2	0	0	0	0	0	0	0
..GTACTACGATGCCATGGC......................................................................................................................................................................................................................................	18	0	1	1.00	1	0	1	0	0	0	0	0	0
...............................................................................................................................................................................TGGCACAGTAGTGATTGTGGTT.....................................................	22	3	2	0.50	1	0	0	1	0	0	0	0	0
..............................................................................TACTAGCTTATGAAATGGA.........................................................................................................................................................	19	2	2	0.50	1	0	0	1	0	0	0	0	0
..........................................................................................................................................................................................................................TATTTCTTGTTATTTCTGGA............	20	2	4	0.25	1	0	0	0	1	0	0	0	0
..................................................GTAAGACGACTCTTGGCCT.....................................................................................................................................................................................	19	3	10	0.10	1	0	0	0	0	1	0	0	0
...........................TAGGGGGTCATTCGA................................................................................................................................................................................................................	15	2	20	0.05	1	0	0	0	0	0	0	1	0
...................................................................................................................................................ATTGTCAATTTTGGA........................................................................................	15	1	20	0.05	1	0	0	0	0	0	1	0	0
....................................................................................................................................................................................................................................TATTTCTGTACTCGATA.....	17	2	20	0.05	1	0	0	0	0	0	0	0	1

CTCATGATGCTACGGTACCGGAAGGAGTTCCTCCAGTAAGCTCTTGCGTTCATTCTGCTGCAAACTGGACGGGGTTCTATGATCGAATACTCTATCTATGGCAAATTCCAATCTTCTCGAGTCTCATATCGATCCAACGTAAAGAGATTACAGTTAAAACCTGTAAGGACAATAAACCGTTTCAGCACAAACACCAAAAACCTTGTAAAGCGCTATAAATAAAGAAAAATAAAGACATGGGCAATGATAA ************************************************..(((((.(((..((((.(((((((((((.((((((..((((......(((......)))........))))......))))))...(((((.....)))))..)))))))(((....))).....))))..))))....))).))))).************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	SRR618934 dsim w501 ovaries	SRR553487 NRT_0-2 hours eggs	SRR553486 Makindu_3 day-old ovaries	SRR553488 RT_0-2 hours eggs
................................................TTCATTCTGCTGCAAA..........................................................................................................................................................................................	16	0	1	1.00	1	1	0	0	0
...................................................................................................................CTCGAGTCTCATATCGATCCAACGT..............................................................................................................	25	0	1	1.00	1	1	0	0	0
..AATGATGCTACGGTACCGGAAGGAGTT.............................................................................................................................................................................................................................	27	1	1	1.00	1	1	0	0	0
...............................TCCCGTCAGCTCTTGCGT.........................................................................................................................................................................................................	18	2	5	0.60	3	0	3	0	0
...................................................................................................AGCAAATACCAATCTT.......................................................................................................................................	16	2	20	0.35	7	1	0	6	0
..................................................................................................TAGCAAATACCAATCTT.......................................................................................................................................	17	2	20	0.25	5	5	0	0	0
...........................................................................................................................TCATATCGATGCAAGGT..............................................................................................................	17	2	7	0.14	1	1	0	0	0
....................................................................................................................................................................................................AAAACCTGGTAAAGAGCTA...................................	19	2	18	0.06	1	0	0	0	1
.....................AACGAGTTCCTCCATTAT...................................................................................................................................................................................................................	18	3	20	0.05	1	1	0	0	0

Species	Coordinate	ID	Alignment
droSim2	3l:22393122-22393371 +	dsi_20377	GAGTACTA-CGATGCCATGGCCTT--CCTCAAGGAGGTCATTCGAGAACGCAAG----TAAGAC-GACGTTTGACCTGCCCCAAG------------ATACTAGCTTATGA--------GATAGA----TACCGTTTAAGGTTAGAAGA-------------------------GCTCAGAGT-ATAGCT-------AGGTTGCATT------------------------------------------------------------------TCTCTAATGTC------------------------------------------------------------AA-------------------------TTTTGGACATT-CCTGTTATTT------------GGCAAAGTC--GTGTTTGTGGTTTTTGGAACATTTCGC---------------------------------------------------------------GATATTT-------------------------------------------------------------------------------------------------------------------------------ATTTCTTTTTATTTC---------------TGTACCC---------GTTACTATT
droSec2	scaffold_11:2807338-2807615 +		GAATACTA-CGATGCCATGGCCTT--CCTCAAGGAGGTCATTCGAGAACGCAAG----TAAGAT-GACGTTTGACCTGCCCCAAG------------ATACTAGCTTATGA--------GATAGG----TACCGATTAAGGTTAAAAGA-------------------------GTTGAGGGT-ATAGCG-------AGGTTTCATC------------------------------------------------------------------TCTCCAATGTC------------------------------------------------------------AA-------------------------TTTTGGACATT-CCTGTTATTT------------GGCAAAGTC--GTGTTTGTGGTTTTTGCAACATTTCACGATACGAAAGATAATTAAGCTTTAGTTAGACCATACGGGC---------------------------------------------------------------------------------------------------------------------------TG------------------------------------GATTTTATTTC---------------TATACTC---------GTTA---CT
dm3	chr3L:22905284-22905436 +		GAATACTA-CGATGCCATGGCCTT--CCTCAAGGAGGTCATTCGGGAACGCAAG----TAAGAC-GACGTTTGACCTGCCCTAAA------------ATACTACCTTATGA--------TATATGTAGATCCCGTTTAAGG-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GA-------------------------TTTTGGACATT-CCAGTTATTT------------GT-----------T----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CTAGTGTCTT---------------TG---------------------GA
droEre2	scaffold_4784:22554468-22554661 +		GAATACTA-CGATGCCATGGCCTT--CCTCAAGGAGGTCATTCGGGAGCGCAAG----TAAGAC-GATGTTTGACCTGCCCTAAG------------CTATTAGCTTATCA--------GA-------------------------AGT-------------------------GCTTGGGGT-TCAACT--------GGTAACCTT------------------------------------------------------------------CCTCCAATGTC------------------------------------------------------------CA-------------------------TTTTGCACATT-CCTGTTATTC------------GACGAAATC--GTGTTTGTGTTTTATACTACACGTTGT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GATA
droYak3	v2_chrUn_3594:560-767 +		GAATACTA-CGATGCCATGGCCTT--CCTCAAGGAGGTCATTCGGGAGCGCAAG----TAAGAA-GATATTTGACCTGCCCCAAG------------CTATTAGCTTATGA--------GATA-A----TCCTGCTTAAAGTCAGA--T-------------------------GCTTAGGGT-ATAATT------------ACCTT------------------------------------------------------------------TTTCCAATGTC------------------------------------------------------------CA-------------------------TTTTGGACATT-TCTTTTATTC------------GATTAATTC--GAGTTTGTGGTTTATACAACACGTTGT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GATA
droEug1	scf7180000409589:16724-16860 -		AGCTCGAA-GCTTTT-G---------------------------------------------------------------------------------------TTGT---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TAAGCTGAATTTCTAGCATTTTCGGCGTGGTT----------------------TTGTT--------GCGAAGTT-----------------------------------------GATTTGGTTTTGATTTGAAC--TCGGAATAA-----------AAT---TCGGAAATTT-------------------------------------------------------------------------------------------------------------------------------ACCTGCCCCGGTTTT---------------TATACCC---------GTTACTCGT
droBia1	scf7180000302514:12131-12283 +		TGTGAGGG-GAATTCGG---------------------------------------------------------------------------------------------------------------------TTTCTATTTAGATGA-------------------------GTTTGG-----------------------------------------------------------------------------------------------------------------------------------------------------------C-TATATGTTGAATTATTATTATTTTTGTAATGTTA----------------------TTGTTAATAATA-------TTTTT----TATTGTTGTT---------------------------------------GTAAC--TTTGATTAC--------------------------------------------------------------------------------------------------------------------------G------------------------------------ATTTTTTTTG-----TGTATTT---TCTGAATTCCTTC---GACTATAGT
droTak1	scf7180000414699:7546-7811 -		GCGGAACA-CAATCTCTGGACCTT--TCTGAGCCATTTTGTCTAAGAAACTATGCCTTGAAAAT-CACGTTTGACCCTCCGCAATTGCAAAAGAAAAATACTGGTTTTTCG--------TTTAT---------------TTTGAGAAAA-------------------------AT-ATCA----------TAGTAG----------CCGTTACAGATATTTATCAAATTTAA----------------TCTCTCTTTTTTATTAGAAAATTTGCT-------------------------------------------------CTCCCTTTAGTTTATTTCTTCAAAT-------------------------ATGTTTT-TTTATTTCAT------------AC--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TGTTTTTT---TATTATTTCCCAGACC-----CAAATA---AGACC
droEle1	scf7180000488055:5364-5530 -		ACGCAAG---------------------------------------------------TAAATCATACTTTTAATGTTTTC-AGG------------ATATT-------------------TAGT----TT------------------TTGCAATAGCTGCAAGGGTATATGAACTTCGG-C-TTGGCG-------AAGTTT-GCT------------------------------------------------------------------TCCTTAATTT--------------------------------------------------------------T-------------------------ATTTATATATT-TATTTTATTT------------AATAATATTTATTATTTGTAA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ATTTCTTTTACTTTT---------------TATACC------------------C
droRho1	scf7180000768839:20972-21157 +		GAAATAGCACCGT---------------TTAATAAAATTATAAT-----------------------------------------------------------------------------------------------------------------------------------------GT-A-----AAG-AGG----------TATACGTGTT-------------------TCTTTA---------------ACATTTAAGACATGTTTGCTTGTTCTCTCTGATTCCATTTTGTAAAGTAATGTAAA-----------------------------------------------------------------------------------------------------------ATGTAAAT-----------------------------------------------------------------------------------TTAAATACATATTTTGTTTTATCCGTTTTTAT-----------------------------------------------------------------T------------------------------------TTTTTGGT---CATGTAATTTT---TATACCC---------GTTACTCGT
droFic1	scf7180000453830:115903-115987 -		GAGTACTA-TGACGCTATGGCCTT--TCTAAAGGAAGTCATTCGGGAACGCAAG----TAAGGAGGGCTTCTAGCCAGTTCAGAG------------ACACAAG---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droKik1	scf7180000302384:217458-217611 +		AAATACCC-TGCCA---------GGGTATAAAAATTATAGC------------------------------T-----------------------------------------------------------------------------------------------------------------------------TCGTT-----------------------------------------------------GT-TTTTCAACGTATGTTTATCTACTCTC------------------------------------------------------------CG-------------------------ATATAAGCATTTTTTATTATTC------------TAGAAATTC--GTATTAATTTTAT---GAAAATCG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GG---CGACTATATCT---TATAGCTGCCATAGGAA---CGATC
droAna3	scaffold_13417:5942538-5942735 +		ACGACGT---------------------------------------------------------------TTGATTCGCCCCATA------------TCACAATCCTATGC--------GATAGG----TA------------------TTGTGATTCCAGCATGGCTAATTGAAGTTTGG-CGGTGGC-------------------------GGATCTGTAATGGCCTTCA------------------------------------------------------GCCTTCATTAAGTTCCAAATACTGACATATAATTTTGAGTGAACTTGATTCATTTTTCC-------------------------ATTTCCATATT-TTTTTTATTT------------AA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTTTTT---------------TT-----------------TATTAT
droBip1	scf7180000394386:21990-22160 -		TATCAGTTATTGT---------------TAAAGGA-------------------------------------------------------------------------------------------------------------------------------------------GTTT------------------------TTATTTTTTCTTGT--------CTGATTTTTTGATTTTTG---------------TCATTTTGGACATTTTTGT-------------------------------------------------ATGTATTCAGT-AATATCTTGAGACAT----ACTTTT-------------------------------CCAATAATAATG-------TCTTT------------------------------------------------------TGAC--CTTGGCCAA--------------------------------------------------------------------------------------------------------------------------G-----------GTTTTTGAGATGCTTGTGTG---------------------------------ACCT---------CCTTGTATT
dp5	XR_group5:130483-130603 -		AGTATGGC---------------------------------------------------------------T-----------------------------------------------------------------------------------------------------------------------------GAGTT-----------------------------------------------------TT-TC-------------TC----------------------------------------------------------------------------------------------------------------------CCACTAATAGTA-------TTTTT----AGCTTCGGTT---------------------------------------GCGTT--GCCGGCCGG--------------------------------------------------------------------------------------------------------------------------TACTTTTGCAGAATTATCAAAATGTTTTGTTGGTTTCTTGGTTTTTT---------------TATACCC---------GATA---CT
droPer2	scaffold_51:48987-49156 +		CCAAGTTA-CGAACTAGCGGACTG--TATTAT-----------------------------------------------------------------------------------------------------CTT-----------------------------------------TAATAT-TGATTT--------TATTGTATT------------------------------------------------------------------CAGCCAGGATC------------------------------------------------------------CG-------------------------GTTTTCTCTTT-CCTATTTACT------------TTTGAAATC--ACATAAA-----------------------------------------------------------------------------------------------------TTGGTGCCCCCGTTTTCACATTCCGCAAGATTTCT-----------------------------------------------------------------------------------TCGTTTTCACGCC----------------AAAAA-----CATATATTACCATT
droWil2	scf2_1100000004958:40325-40502 +		ACATGTTC-CAAAAACCAATCTATGACATAGAAATGGTCTTTCTTTGTTTCAAA----AAA-----------------------------------------------------------------------------------------------------------------GCTGA------------TAATAT----------CTTCTACACAGACGTAGCA----------------GAATTTCCC----------------------TACTTG------------------------------------------------------------------------------------------------------------TTCTCT------------GAGCACGTC---------------------------------------------------------------GTCGATTGCTCTTTGGAAAACAC---TTTGAAATTT------------------------------------------------------------------------------------------------------------------------TCCGT-------TGTTCATTTT---------------TTTATCA---------ACA--ACTC
droVir3	scaffold_13050:2210900-2211033 -		ATACTGGCGAATGCTGG--------------------------------------------------------------------------------------------------------------------------------TAGA-------------------------TCGCAGTGT-A-----TTG-CAT----------TTACCGCGTCTATGTTGAT----------------GAGTGGCCT----------------------GATTGT------------------------------------------------------------------------------------------------------------TTTGAA------------TAGGGTGTT---------------------------------------------------------------TTTGTTTGTGAGTTTTATA-------------------------------------------------------AGGTGGCG-----------------------------------------------TA------------------------------------TTTTTTATTTG---------------TATACC-----------------CT
droMoj3	scaffold_6680:23213506-23213637 -		TTAAAAGA-GCT---AC-------AATATG----------GCGATGAAAAAA--------------------------------------------------------------------------------------------------------------------------ACTA------------TTA-----------------------------------------------------------------------------------------------------------------------------------------------------------------CAATTTTCGC-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ATGCCACTCAGTGATTTCAATTTTTTATTAGAAAAAGTACATTTTTTCAGCAATTTCGCGATAAG------------------------------------CATTTTGTTTT---------------TATACCC-----------------T
droGri2	scaffold_15245:6020970-6021109 +		TCAGAGAA-TATCTCGG---------------------------------------------------------------------------------------TTATTCATTTGGTTTTGCCGG----TG------------------CTCTGACATCTC----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TC----------------------TTGTT-------GGCAAAAGG--TTGGCTCTGGTT------------------------------------------TTAGC--CCAGATTAC-----------GTTTGCCAAGAAATTC------------------------------------------------------------------------------------------------------------------------TTTGT------TTTTCTCTTTT---------------TATACCC---------ATTA-AATA

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droSim2

GAGTACTA-CGATGCCATGGCCTT--CCTCAAGGAGGTCATTCGAGAACGCAAG----TAAGAC-GACGTTTGACCTGCCCCAAG------------ATACTAGCTTATGA--------GATAGA----TACCGTTTAAGGTTAGAAGA-------------------------GCTCAGAGT-ATAGCT-------AGGTTGCATT------------------------------------------------------------------TCTCTAATGTC------------------------------------------------------------AA-------------------------TTTTGGACATT-CCTGTTATTT------------GGCAAAGTC--GTGTTTGTGGTTTTTGGAACATTTCGC---------------------------------------------------------------GATATTT-------------------------------------------------------------------------------------------------------------------------------ATTTCTTTTTATTTC---------------TGTACCC---------GTTACTATT	Size	Hit Count	Total Norm	Total	M023 Head	M024 Male-body	M025 Embryo	M053 Female-body	O001 Testis	O002 Head	SRR553485 Ovary	SRR553486 Ovary	SRR553487 Ovary	SRR553488 Ovary	SRR618934 Ovary	V044 Embryo	SRR902008 Ovary	SRR902009 Testis	SRR1275487 Male larvae	SRR1275485 Male prepupae	SRR1275483 Male prepupae
..........................................................................................................................................................................................................................................................................................................................................................................................................................................................C---------------------------------------------------------------GATATTT-------------------------------------------------------------------------------------------------------------------------------ATTTCTTTTT.............................................	18	1	2.00	2	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0
..GTACTA-CGATGCCATGGC..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	18	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0

droSec2

GAATACTA-CGATGCCATGGCCTT--CCTCAAGGAGGTCATTCGAGAACGCAAG----TAAGAT-GACGTTTGACCTGCCCCAAG------------ATACTAGCTTATGA--------GATAGG----TACCGATTAAGGTTAAAAGA-------------------------GTTGAGGGT-ATAGCG-------AGGTTTCATC------------------------------------------------------------------TCTCCAATGTC------------------------------------------------------------AA-------------------------TTTTGGACATT-CCTGTTATTT------------GGCAAAGTC--GTGTTTGTGGTTTTTGCAACATTTCACGATACGAAAGATAATTAAGCTTTAGTTAGACCATACGGGC---------------------------------------------------------------------------------------------------------------------------TG------------------------------------GATTTTATTTC---------------TATACTC---------GTTA---CT	Size	Hit Count	Total Norm	Total	M054 Female-body	V113 Male-body	V114 Embryo	V115 Head
No Reads

dm3

GAATACTA-CGATGCCATGGCCTT--CCTCAAGGAGGTCATTCGGGAACGCAAG----TAAGAC-GACGTTTGACCTGCCCTAAA------------ATACTACCTTATGA--------TATATGTAGATCCCGTTTAAGG-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GA-------------------------TTTTGGACATT-CCAGTTATTT------------GT-----------T----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CTAGTGTCTT---------------TG---------------------GA	Size	Hit Count	Total Norm	Total	GSM154618 Ovary	GSM154620 Ovary	GSM154621 Ovary	GSM154622 Ovary	GSM180328 Head	GSM180329 Mixed-body	GSM180330 Embryo	GSM180331 Embryo	GSM180332 Embryo	GSM180333 Embryo	GSM180334 Larvae	GSM180335 Disc	GSM180336 Pupae	GSM180337 S2	GSM231091 Ovary	GSM239041 Head	GSM239050 Head	GSM239051 S2	GSM239052 S2	GSM246084 Head	GSM266765 S2	GSM280082 Ovary	GSM280083 Ovary	GSM280084 Ovary	GSM280085 Testis	GSM280086 Ovary	GSM280087 S2-NP	GSM280088 S2-NP	GSM312995 Head	GSM313160 Head	GSM313161 Head	GSM313162 Head	GSM313163 Head	GSM313164 Head	GSM313165 Head	GSM343287 Embryo	GSM379050 Ovary	GSM379051 Ovary	GSM379052 Ovary	GSM379053 Ovary	GSM379054 Ovary	GSM379055 Ovary	GSM379056 Ovary	GSM379057 Ovary	GSM379058 Ovary	GSM379059 Ovary	GSM379060 Ovary	GSM379061 Ovary	GSM379062 Ovary	GSM379063 Ovary	GSM379064 Ovary	GSM379065 Ovary	GSM379066 Ovary	GSM379067 Ovary	GSM424739 S2	GSM424740 S2	GSM424741 S2	GSM467729 Female-body	GSM467730 Female-body	GSM467731 Female-body	M002 CS	M003 Male-body	M004 Male-body	M005 ML-DmD32	M006 ML-DmD32	M029 OSC	M030 OSC	M031 OSC	M032 S2-R+	M033 S2-R+	M034 S2-R+	M035 S2-R+	M036 S2-R+	M037 S2-R+	M038 S2-R+	M039 S2-R+	M048 S2-R+	OSS2 OSS	OSS6 OSS	OSS7 OSS	OSS8 OSS	S1 Head	S10 Embryo	S11 Embryo	S12 Embryo	S13 Embryo	S14 Embryo	S15 S2-GMR	S16 Kc	S17 Larvae	S18 Larvae	S19 Pupae	S2 Head	S20 Pupae	S21 Disc	S22 Head	S23 Female-body	S24 Male-body	S25 Larvae	S26 Larvae	S27 Pupae	S28 Pupae	S29 Head	S3 Male-body	S30 S2-GMR	S31 S2	S32 S2	S33 Kc	S34 Kc	S35 Kc	S4 Female-body	S5 Disc	S6 Embryo	S7 Embryo	S8 S2	S9 Embryo	SRR001337 Head	SRR001338 Head	SRR001339 Head	SRR001340 Head	SRR001341 Head	SRR001342 Head	SRR001343 Head	SRR001344 Head	SRR001345 Head	SRR001346 Head	SRR001347 Head	SRR001348 Head	SRR001349 Head	SRR001664 Head	SRR010951 Ovary	SRR010952 Ovary	SRR010953 Ovary	SRR010954 Ovary	SRR010955 Ovary	SRR010956 Ovary	SRR010957 Ovary	SRR010958 Ovary	SRR010959 Ovary	SRR010960 Ovary	SRR014268 Embryo	SRR014269 Embryo	SRR014270 Embryo	SRR014271 Embryo	SRR014272 Embryo	SRR014273 Ovary	SRR014274 Ovary	SRR014275 Ovary	SRR014276 Ovary	SRR014277 Ovary	SRR014278 Ovary	SRR014279 Ovary	SRR014280 Ovary	SRR014281 Ovary	SRR014282 Ovary	SRR023197 S2	SRR023399 S2	SRR023400 S2	SRR023402 S2	SRR023407 S2	SRR029028 S2	SRR029029 S2	SRR029030 S2	SRR029031 S2	SRR029032 S2	SRR029033 S2	SRR029608 Head	SRR029633 Head	SRR030145 gb3	SRR031692 Head	SRR031693 Head	SRR031694 Head	SRR031696 Head	SRR031697 Head	SRR031698 Head	SRR031699 Head	SRR031700 Head	SRR031701 Head	SRR031702 Head	SRR031703 Head	SRR031704 Head	SRR032092 S2	SRR032093 S2	SRR032094 S2	SRR032095 S2-NP	SRR032096 S2-NP	SRR032152 Head	SRR060643 Testis	SRR060644 Ovary	SRR060645 Testis	SRR060646 Ovary	SRR060647 Ovary	SRR060648 Ovary	SRR060649 Ovary	SRR060650 Testis	SRR060651 Ovary	SRR060652 Testis	SRR060653 Ovary	SRR065151 Ovary	SRR065152 Ovary	SRR065153 Ovary	SRR065154 Ovary	SRR065155 Ovary	SRR065800 Ovary	SRR065801 Ovary	SRR065802 Ovary	SRR065803 Ovary	SRR065804 Ovary	SRR065805 Ovary	SRR065806 Ovary	SRR065807 Ovary	SRR097865 S2-NP	SRR097866 S2-NP	SRR097867 S2-NP	SRR298536 Ovary	SRR298537 Ovary	SRR298711 Ovary	SRR298712 Ovary	SRR317110 Ovary	SRR317111 Ovary	SRR317112 Ovary	SRR317113 Ovary	SRR317114 Ovary	SRR317115 Ovary	SRR317116 Ovary	SRR317117 Ovary	SRR341115 S2	SRR341116 S2	SRR341117 S2	SRR341118 S2	SRR351332 Mixed-body	SRR351333 Mixed-body	T004 OSC	T005 CME-L1	V001 Embryo	V002 Embryo	V003 S2	V006 Female-body	V007 Head	V008 S2	V009 CME-L1	V010 ML-DmD20-C5	V011 Sg4	V012 Male-body	V013 Head	V014 DTT	V015 DreRFHV	V018 Kc167	V019 GM2	V020 S2-R+	V021 ML-DmD21	V022 ML-DmD32	V023 Head	V024 Kc167	V025 S2-R+	V026 1182-2H	V027 ML-DmD21	V028 CME-W1-Cl.8+	V029 ML-DmD9	V030 ML-DmD8	V031 GM2	V032 S1	V033 CME-W1-Cl.8+	V034 ML-DmD16-C3	V035 ML-DmD32	V036 ML-DmD20-C5	V037 S2	V038 S2	V063 Ovary	V0632 Ovary	V064 Ovary	V0642 Ovary	V065 Ovary	V0652 Ovary	V066 Ovary	V0662 Ovary	V067 S2-NP	V073 mbn2	V074 S3	V075 ML-DmBG1-C1	V076 ML-DmBG3-C2	V077 Head	V078 Head	V079 Head	V080 Head	V081 Embryo	V082 Embryo	V083 Male-body	V084 Female-body	V085 CME-W2	V086 Female-body	V088 Ovary	V0882 Ovary	V089 Ovary	V0892 Ovary	V090 Male-body	V091 fGS/OSS	V092 Male-body	V093 Male-body	V094 S2-R+	V095 S2-R+	V096 Ovary	V097 Ovary	V098 Ovary	V125 ML-DmD9	V126 CME-L1	V127 G2	V128 S3	V129 ML-DmBG1-C1	V130 ML-DmBG3-C2	V131 ML-DmD16-C3	V132 ML-DmD32	V133 Sg4	V134 ML-DmD8	V135 CME-W2	V136 Male-body	V137 Head	V138 Male-body	V139 Female-body	V140 Female-body	V141 Female-body	V142 Female-body	V144 OSC	V145 S2	V146 S1	V147 1182-2H	V148 mbn2	GSM1528798 follicle cells
..............................AAGGAGGTCATTCGGGAACGCAAG----.............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	24	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
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................................GGAGGTCATTCGGGAACGCA...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
............TGCCATGGCCTT--CCTCAAGGAGGTC................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	25	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
GAATACTA-CGATGCCAGT....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	18	4	0.25	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

droEre2

GAATACTA-CGATGCCATGGCCTT--CCTCAAGGAGGTCATTCGGGAGCGCAAG----TAAGAC-GATGTTTGACCTGCCCTAAG------------CTATTAGCTTATCA--------GA-------------------------AGT-------------------------GCTTGGGGT-TCAACT--------GGTAACCTT------------------------------------------------------------------CCTCCAATGTC------------------------------------------------------------CA-------------------------TTTTGCACATT-CCTGTTATTC------------GACGAAATC--GTGTTTGTGTTTTATACTACACGTTGT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GATA	Size	Hit Count	Total Norm	Total	M055 Female-body	M057 Embryo	V060 Head	V040 Embryo	V107 Male-body	V108 Head	GSM1528801 follicle cells
No Reads

droYak3

GAATACTA-CGATGCCATGGCCTT--CCTCAAGGAGGTCATTCGGGAGCGCAAG----TAAGAA-GATATTTGACCTGCCCCAAG------------CTATTAGCTTATGA--------GATA-A----TCCTGCTTAAAGTCAGA--T-------------------------GCTTAGGGT-ATAATT------------ACCTT------------------------------------------------------------------TTTCCAATGTC------------------------------------------------------------CA-------------------------TTTTGGACATT-TCTTTTATTC------------GATTAATTC--GAGTTTGTGGTTTATACAACACGTTGT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GATA

Size

Hit Count

Total Norm

Total

M026
Head

M043
Female-body

M056
Embryo

V046
Embryo

V052
Head

V058
Head

V120
Male-body

SRR1275488
Male larvae

SRR1275486
Male prepupae

SRR1275484
Male prepupae

GSM1528802
follicle cells

No Reads

droEug1

AGCTCGAA-GCTTTT-G---------------------------------------------------------------------------------------TTGT---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TAAGCTGAATTTCTAGCATTTTCGGCGTGGTT----------------------TTGTT--------GCGAAGTT-----------------------------------------GATTTGGTTTTGATTTGAAC--TCGGAATAA-----------AAT---TCGGAAATTT-------------------------------------------------------------------------------------------------------------------------------ACCTGCCCCGGTTTT---------------TATACCC---------GTTACTCGT	Size	Hit Count	Total Norm	Total
No Reads

droBia1

TGTGAGGG-GAATTCGG---------------------------------------------------------------------------------------------------------------------TTTCTATTTAGATGA-------------------------GTTTGG-----------------------------------------------------------------------------------------------------------------------------------------------------------C-TATATGTTGAATTATTATTATTTTTGTAATGTTA----------------------TTGTTAATAATA-------TTTTT----TATTGTTGTT---------------------------------------GTAAC--TTTGATTAC--------------------------------------------------------------------------------------------------------------------------G------------------------------------ATTTTTTTTG-----TGTATTT---TCTGAATTCCTTC---GACTATAGT	Size	Hit Count	Total Norm	Total
No Reads

droTak1

GCGGAACA-CAATCTCTGGACCTT--TCTGAGCCATTTTGTCTAAGAAACTATGCCTTGAAAAT-CACGTTTGACCCTCCGCAATTGCAAAAGAAAAATACTGGTTTTTCG--------TTTAT---------------TTTGAGAAAA-------------------------AT-ATCA----------TAGTAG----------CCGTTACAGATATTTATCAAATTTAA----------------TCTCTCTTTTTTATTAGAAAATTTGCT-------------------------------------------------CTCCCTTTAGTTTATTTCTTCAAAT-------------------------ATGTTTT-TTTATTTCAT------------AC--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TGTTTTTT---TATTATTTCCCAGACC-----CAAATA---AGACC	Size	Hit Count	Total Norm	Total
No Reads

droEle1

ACGCAAG---------------------------------------------------TAAATCATACTTTTAATGTTTTC-AGG------------ATATT-------------------TAGT----TT------------------TTGCAATAGCTGCAAGGGTATATGAACTTCGG-C-TTGGCG-------AAGTTT-GCT------------------------------------------------------------------TCCTTAATTT--------------------------------------------------------------T-------------------------ATTTATATATT-TATTTTATTT------------AATAATATTTATTATTTGTAA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ATTTCTTTTACTTTT---------------TATACC------------------C	Size	Hit Count	Total Norm	Total
No Reads

droRho1

GAAATAGCACCGT---------------TTAATAAAATTATAAT-----------------------------------------------------------------------------------------------------------------------------------------GT-A-----AAG-AGG----------TATACGTGTT-------------------TCTTTA---------------ACATTTAAGACATGTTTGCTTGTTCTCTCTGATTCCATTTTGTAAAGTAATGTAAA-----------------------------------------------------------------------------------------------------------ATGTAAAT-----------------------------------------------------------------------------------TTAAATACATATTTTGTTTTATCCGTTTTTAT-----------------------------------------------------------------T------------------------------------TTTTTGGT---CATGTAATTTT---TATACCC---------GTTACTCGT	Size	Hit Count	Total Norm	Total
No Reads

droFic1

GAGTACTA-TGACGCTATGGCCTT--TCTAAAGGAAGTCATTCGGGAACGCAAG----TAAGGAGGGCTTCTAGCCAGTTCAGAG------------ACACAAG---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droKik1

AAATACCC-TGCCA---------GGGTATAAAAATTATAGC------------------------------T-----------------------------------------------------------------------------------------------------------------------------TCGTT-----------------------------------------------------GT-TTTTCAACGTATGTTTATCTACTCTC------------------------------------------------------------CG-------------------------ATATAAGCATTTTTTATTATTC------------TAGAAATTC--GTATTAATTTTAT---GAAAATCG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GG---CGACTATATCT---TATAGCTGCCATAGGAA---CGATC	Size	Hit Count	Total Norm	Total
No Reads

droAna3

ACGACGT---------------------------------------------------------------TTGATTCGCCCCATA------------TCACAATCCTATGC--------GATAGG----TA------------------TTGTGATTCCAGCATGGCTAATTGAAGTTTGG-CGGTGGC-------------------------GGATCTGTAATGGCCTTCA------------------------------------------------------GCCTTCATTAAGTTCCAAATACTGACATATAATTTTGAGTGAACTTGATTCATTTTTCC-------------------------ATTTCCATATT-TTTTTTATTT------------AA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTTTTT---------------TT-----------------TATTAT	Size	Hit Count	Total Norm	Total	M044 Female-body	M058 Embryo	V039 Embryo	V055 Head	V105 Male-body	V106 Head
................................................................................................................................................................GCATGGCTAATTGAAGTTTGG-CGG..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	24	2	0.50	1	0	0	1	0	0	0
................................................................................................................................................................GCATGGCTAATTGAAGTTTGG-C................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	22	2	0.50	1	0	1	0	0	0	0
........................................................................................................................................................................................................................................................................................................AAGTTCCAAATACTGACATATAATT......................................................................................................................................................................................................................................................................................................................................................................................	25	2	0.50	1	0	1	0	0	0	0
....CGT---------------------------------------------------------------TTGATTCGCCCCATA------------TCACAATCC.............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	27	20	0.30	6	6	0	0	0	0	0
.CGACGT---------------------------------------------------------------TTGATTCGCCCCATA------------TCAC..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	25	20	0.15	3	3	0	0	0	0	0
.......................................................................TGATTCGCCCCATA------------TCACAATCCTATG.........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	27	20	0.15	3	3	0	0	0	0	0
.......................................................................TGATTCGCCCCATA------------TCACAATCCTATGC--------................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	28	7	0.14	1	0	1	0	0	0	0
......................................................................TTGATTCGCCCCATA------------TCACAATCCTATG.........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	28	20	0.10	2	0	2	0	0	0	0
......T---------------------------------------------------------------TTGATTCGCCCCATA------------TCACAATCC.............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	25	20	0.10	2	2	0	0	0	0	0
........................................................................GATTCGCCCCATA------------TCACAATCCTATG.........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	26	20	0.10	2	2	0	0	0	0	0
.CGACGT---------------------------------------------------------------TTGATTCGCCCCATA------------TCACA.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	26	20	0.10	2	2	0	0	0	0	0
......T---------------------------------------------------------------TTGATTCGCCCCATA------------TCACAATCCTATG.........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	29	20	0.10	2	2	0	0	0	0	0
......T---------------------------------------------------------------TTGATTCGCCCCATA------------TCACAATCCTA...........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	27	20	0.05	1	1	0	0	0	0	0
..........................................................................TTCGCCCCATA------------TCACAATCCT............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	21	20	0.05	1	1	0	0	0	0	0
.....GT---------------------------------------------------------------TTGATTCGCCCCATA------------TCACAATCC.............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	26	20	0.05	1	1	0	0	0	0	0
.CGACGT---------------------------------------------------------------TTGATTCGCCCCATA------------TCACAAT...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	28	20	0.05	1	1	0	0	0	0	0
....CGT---------------------------------------------------------------TTGATTCGCCCCATA------------TCACAATCCTA...........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	29	20	0.05	1	1	0	0	0	0	0
ACGACGT---------------------------------------------------------------TTGATTCGCCCCATA------------TCACA.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	27	20	0.05	1	1	0	0	0	0	0

droBip1

TATCAGTTATTGT---------------TAAAGGA-------------------------------------------------------------------------------------------------------------------------------------------GTTT------------------------TTATTTTTTCTTGT--------CTGATTTTTTGATTTTTG---------------TCATTTTGGACATTTTTGT-------------------------------------------------ATGTATTCAGT-AATATCTTGAGACAT----ACTTTT-------------------------------CCAATAATAATG-------TCTTT------------------------------------------------------TGAC--CTTGGCCAA--------------------------------------------------------------------------------------------------------------------------G-----------GTTTTTGAGATGCTTGTGTG---------------------------------ACCT---------CCTTGTATT	Size	Hit Count	Total Norm	Total
No Reads

dp5

AGTATGGC---------------------------------------------------------------T-----------------------------------------------------------------------------------------------------------------------------GAGTT-----------------------------------------------------TT-TC-------------TC----------------------------------------------------------------------------------------------------------------------CCACTAATAGTA-------TTTTT----AGCTTCGGTT---------------------------------------GCGTT--GCCGGCCGG--------------------------------------------------------------------------------------------------------------------------TACTTTTGCAGAATTATCAAAATGTTTTGTTGGTTTCTTGGTTTTTT---------------TATACCC---------GATA---CT	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
.....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TTGCAGAATTATCAAAATGTTTT...........................................................	23	1	2.00	2	0	0	0	0	2	0	0	0	0	0
................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TCAAAATGTTTTGTTGGTTTCTTGG..............................................	25	1	1.00	1	0	0	0	0	0	0	0	1	0	0
......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TGGTTTTTT---------------TATACCC---------GATA---CC	22	2	0.50	1	0	1	0	0	0	0	0	0	0	0

droPer2

CCAAGTTA-CGAACTAGCGGACTG--TATTAT-----------------------------------------------------------------------------------------------------CTT-----------------------------------------TAATAT-TGATTT--------TATTGTATT------------------------------------------------------------------CAGCCAGGATC------------------------------------------------------------CG-------------------------GTTTTCTCTTT-CCTATTTACT------------TTTGAAATC--ACATAAA-----------------------------------------------------------------------------------------------------TTGGTGCCCCCGTTTTCACATTCCGCAAGATTTCT-----------------------------------------------------------------------------------TCGTTTTCACGCC----------------AAAAA-----CATATATTACCATT	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TTCCGCAAGATTTCT-----------------------------------------------------------------------------------TCGTTTTCA............................................	24	20	0.70	14	0	14	0	0	0	0
.....................................................................................................................................................................................................................................................................................................................................................................................................TACT------------TTTGAAATC--ACATAAA-----------------------------------------------------------------------------------------------------TTGG.......................................................................................................................................................................	24	20	0.20	4	0	4	0	0	0	0
................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TTCCGCAAGATTTCT-----------------------------------------------------------------------------------TCGTTTTC.............................................	23	20	0.15	3	0	3	0	0	0	0
...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ATTCCGCAAGATTTCT-----------------------------------------------------------------------------------TCGT.................................................	20	20	0.10	2	2	0	0	0	0	0
...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ATTCCGCAAGATTTCT-----------------------------------------------------------------------------------TCGTTTTC.............................................	24	20	0.05	1	1	0	0	0	0	0
...................................................................................................................................................................................................................................................................................................................................................................................................TTTACT------------TTTGAAATC--ACATAAA-----------------------------------------------------------------------------------------------------TT.........................................................................................................................................................................	24	20	0.05	1	0	1	0	0	0	0
....................................................................................................................................................................................................................................................................................................................................................................................................TTACT------------TTTGAAATC--ACATAAA-----------------------------------------------------------------------------------------------------TTG........................................................................................................................................................................	24	20	0.05	1	0	1	0	0	0	0
................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TTCCGCAAGATTTCT-----------------------------------------------------------------------------------TCG..................................................	18	20	0.05	1	0	1	0	0	0	0
..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CATTCCGCAAGATTTCT-----------------------------------------------------------------------------------TCGTTT...............................................	23	20	0.05	1	0	1	0	0	0	0

droWil2

ACATGTTC-CAAAAACCAATCTATGACATAGAAATGGTCTTTCTTTGTTTCAAA----AAA-----------------------------------------------------------------------------------------------------------------GCTGA------------TAATAT----------CTTCTACACAGACGTAGCA----------------GAATTTCCC----------------------TACTTG------------------------------------------------------------------------------------------------------------TTCTCT------------GAGCACGTC---------------------------------------------------------------GTCGATTGCTCTTTGGAAAACAC---TTTGAAATTT------------------------------------------------------------------------------------------------------------------------TCCGT-------TGTTCATTTT---------------TTTATCA---------ACA--ACTC	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
No Reads

droVir3

ATACTGGCGAATGCTGG--------------------------------------------------------------------------------------------------------------------------------TAGA-------------------------TCGCAGTGT-A-----TTG-CAT----------TTACCGCGTCTATGTTGAT----------------GAGTGGCCT----------------------GATTGT------------------------------------------------------------------------------------------------------------TTTGAA------------TAGGGTGTT---------------------------------------------------------------TTTGTTTGTGAGTTTTATA-------------------------------------------------------AGGTGGCG-----------------------------------------------TA------------------------------------TTTTTTATTTG---------------TATACC-----------------CT	Size	Hit Count	Total Norm	Total	M027 Male-body	M028 Head	M047 Female-body	M061 Embryo	SRR060654 Ovary	SRR060655 Testis	SRR060656 Ovary	SRR060657 Testis	SRR060658 Ovary	SRR060659 Testis	SRR060660 Ovary	SRR060661 Embryo	SRR060662 Embryo	SRR060663 Embryo	SRR060664 Male-body	SRR060665 Female-body	SRR060666 Male-body	SRR060667 Female-body	SRR060668 Male-body	SRR060669 Female-body	SRR060670 Testis	SRR060671 Male-body	SRR060672 Female-body	SRR060673 Ovary	SRR060674 Ovary	SRR060675 Ovary	SRR060676 Ovary	SRR060677 Ovary	SRR060678 Testis	SRR060679 Testis	SRR060680 Testis	SRR060681 Testis	SRR060682 Embryo	SRR060683 Testis	SRR060684 Embryo	SRR060685 Embryo	SRR060686 Embryo	SRR060687 Embryo	SRR060688 Ovary	SRR060689 Testis	SRR1106712 embryo_0-2h	SRR1106713 embryo_0-2h	SRR1106714 embryo_2-4h	SRR1106715 embryo_4-6h	SRR1106716 embryo_4-6h	SRR1106717 embryo_6-8h	SRR1106718 embryo_6-8h	SRR1106719 embryo_8-10h	SRR1106720 embryo_8-10h	SRR1106721 embryo_10-12h	SRR1106722 embryo_10-12h	SRR1106723 embryo_12-14h	SRR1106724 embryo_12-14h	SRR1106725 embryo_14-16h	SRR1106726 embryo_14-16h	SRR1106727 larvae	SRR1106728 larvae	SRR1106729 mixed whole adult body	SRR1106730 embryo_16-30h	V047 Embryo	V053 Head	V116 Male-body	GSM1528803 follicle cells
...............................................................................................................................................................................CGCAGTGT-A-----TTG-CAT----------TTACCGCGTCT.............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	26	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...................................................................................................................................................................................................................CGCGTCTATGTTGAT----------------GAGTGG...............................................................................................................................................................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..............................................................................................................................................................................................................................TGAT----------------GAGTGGCCT----------------------GATTGT------------------------------------------------------------------------------------------------------------TTTGA...............................................................................................................................................................................................................................................................................................................	24	2	1.00	2	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TATA-------------------------------------------------------AGGTGGCG-----------------------------------------------TA------------------------------------TTTTTTATTTG---------------TA.......................	27	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..............................................................................................................................................................................................................................TGAT----------------GAGTGGCCT----------------------GATTGT------------------------------------------------------------------------------------------------------------TTTGAA------------..................................................................................................................................................................................................................................................................................................	25	2	1.00	2	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...............................................................................................................................................................................................................................GAT----------------GAGTGGCCT----------------------GATTGT------------------------------------------------------------------------------------------------------------TTTGAA------------T.................................................................................................................................................................................................................................................................................................	25	2	1.00	2	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..............................................................................................................................................................................................................................TGAT----------------GAGTGGCCT----------------------GATTGT------------------------------------------------------------------------------------------------------------TTTGAA------------T.................................................................................................................................................................................................................................................................................................	26	2	1.00	2	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TATA-------------------------------------------------------AGGTGGCG-----------------------------------------------TA------------------------------------TTTTTTATTTG---------------TC.......................	27	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.................................................................................................................................................................................................................ACCGCGTCTATGTTGAT----------------GAGTGGCC.............................................................................................................................................................................................................................................................................................................................................................................................................................................................	25	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...........................................................................................................................................................................................................................TGTTGAT----------------GAGTGGCCT----------------------GATTG.................................................................................................................................................................................................................................................................................................................................................................................................................................	21	6	0.33	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..............................................................................................................................................................................................................................TGAT----------------GAGTGGCCT----------------------GATTGT------------------------------------------------------------------------------------------------------------TT..................................................................................................................................................................................................................................................................................................................	21	6	0.17	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

droMoj3

TTAAAAGA-GCT---AC-------AATATG----------GCGATGAAAAAA--------------------------------------------------------------------------------------------------------------------------ACTA------------TTA-----------------------------------------------------------------------------------------------------------------------------------------------------------------CAATTTTCGC-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ATGCCACTCAGTGATTTCAATTTTTTATTAGAAAAAGTACATTTTTTCAGCAATTTCGCGATAAG------------------------------------CATTTTGTTTT---------------TATACCC-----------------T	Size	Hit Count	Total Norm	Total	M046 Female-body	V041 Embryo	V049 Head	V056 Head	M060 Embryo	V110 Male-body
.....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CTCAGTGATTTCAATTTTTTATTA..........................................................................................................................	24	1	1.00	1	1	0	0	0	0	0
.....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TTCGCGATAAG------------------------------------CATTTTGTTTT---------------TAT......................	25	1	1.00	1	1	0	0	0	0	0
...........T---AC-------AATATG----------GCGATGAAAAAA--------------------------------------------------------------------------------------------------------------------------ACTA------------AA.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	27	1	1.00	1	1	0	0	0	0	0
.......................................................................................................................................................................................................................................................................................................................................................................TTCGC-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ATGCCACTCAGTGATTTCA.....................................................................................................................................	24	1	1.00	1	1	0	0	0	0	0
.TAAAAGA-GCT---AC-------AATATG----------GCGATG.........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	24	5	0.80	4	3	0	0	1	0	0
....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TACATTTTTTCAGCAATTTCGCGATA.........................................................................................	26	3	0.67	2	2	0	0	0	0	0
.TAAAAGA-GCT---AC-------AATATG----------GCGATGA........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	25	5	0.40	2	2	0	0	0	0	0
.TAAAAGA-GCT---AC-------AATATG----------GC.............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	20	6	0.33	2	1	0	0	0	0	1
....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TACATTTTTTCAGCAATTTCGCGAT..........................................................................................	25	3	0.33	1	0	0	0	0	1	0
......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TCAGTGATTTCAATTTTTTATTAGAA.......................................................................................................................	26	12	0.25	3	3	0	0	0	0	0
.TAAAAGA-GCT---AC-------AATATG----------GCGATGAAA......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	27	4	0.25	1	1	0	0	0	0	0
............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TTCAGCAATTTCGCGATAAG------------------------------------CATTT..............................................	25	4	0.25	1	1	0	0	0	0	0
.....AGA-GCT---AC-------AATATG----------GCGATG.........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	20	5	0.20	1	1	0	0	0	0	0
...AAAGA-GCT---AC-------AATATG----------GCGATG.........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	22	5	0.20	1	0	1	0	0	0	0
......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TCAGTGATTTCAATTTTTTATTAG.........................................................................................................................	24	12	0.17	2	1	1	0	0	0	0

droGri2

TCAGAGAA-TATCTCGG---------------------------------------------------------------------------------------TTATTCATTTGGTTTTGCCGG----TG------------------CTCTGACATCTC----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TC----------------------TTGTT-------GGCAAAAGG--TTGGCTCTGGTT------------------------------------------TTAGC--CCAGATTAC-----------GTTTGCCAAGAAATTC------------------------------------------------------------------------------------------------------------------------TTTGT------TTTTCTCTTTT---------------TATACCC---------ATTA-AATA	Size	Hit Count	Total Norm	Total	M041 Female-body	V109 Male-body
No Reads

dsi_20377

3l:22393172-22393321 +

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads