dsi_15596

exon [3l_8572982_8573697_+]; CDS [3l_8572982_8573697_+]; intron [3l_8573698_8574018_+]

##################################################-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- GATATGGCCAAGTTCAAGACCAAAGATGACGTGGCCGATGACGAGTTGAAGTGAGTTCATAAGTCTCAATTAATTAACCAATTCTTATCGGAAGACGTTTAATAATATTAACATGACATTTAGTATTTACTTTGTTGTTTCAAACATATTTGATATGATAAATCAAACCACGGGGAAATCTTATCTCTGCTAGTATGACCTGAATTTTCTGATGAACACTTGATTTACAGCTAGGAGATTGTAGTTTCCT ************************************************((((((...(((((.(((((((...(((...((((....))))...)))...)))).......))).)))))..))))))((((((((.(((((....))))).))))))))(((.((..((((((.......))))))...)).)))..************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M025 embryo	M053 female body	M024 male body	GSM343915 embryo	SRR618934 dsim w501 ovaries	SRR902008 ovaries	SRR553488 RT_0-2 hours eggs	SRR553487 NRT_0-2 hours eggs
............................................................................................................................................................................................................TTTTCTGATGAACACTTGATT.........................	21	0	1	4.00	4	3	0	0	1	0	0	0	0
............................................................................................................................................................................................................TTTTCTGATGAACACTTGATTT........................	22	0	1	2.00	2	2	0	0	0	0	0	0	0
...................CCAAAGATGACGTGGCCGATGACGAG.............................................................................................................................................................................................................	26	0	1	1.00	1	0	0	0	0	0	1	0	0
.....................................................................................................................................GTTGTTTCAAACATATTTGATATGATA..........................................................................................	27	0	1	1.00	1	0	1	0	0	0	0	0	0
.....GGCCAAGTTCAAGACCAAAGAT...............................................................................................................................................................................................................................	22	0	1	1.00	1	0	1	0	0	0	0	0	0
......................AAGATGACGTGGCCGATGACGAGT............................................................................................................................................................................................................	24	0	1	1.00	1	0	0	0	0	1	0	0	0
...........GTTCAAGACCAAAGATGACGTGGCC......................................................................................................................................................................................................................	25	0	1	1.00	1	0	0	1	0	0	0	0	0
GATATGGCCAAGTTCAAG........................................................................................................................................................................................................................................	18	0	1	1.00	1	0	1	0	0	0	0	0	0
..........................TGACGTGGCCGATGACGA..............................................................................................................................................................................................................	18	0	1	1.00	1	0	1	0	0	0	0	0	0
............................................................................................................................................................................................................TTTTCTGATGAACAATTGAAC.........................	21	3	6	0.17	1	0	0	0	0	0	0	1	0
......................................................................................................................................................................................................................AACAGTTGATTTACA.....................	15	1	20	0.05	1	0	0	0	0	0	0	0	1
......CGCAAGTACAAGACCAAAG.................................................................................................................................................................................................................................	19	3	20	0.05	1	0	0	1	0	0	0	0	0
.......................................................................................................AATATTAGCACGACATTT.................................................................................................................................	18	2	20	0.05	1	0	0	0	1	0	0	0	0

CTATACCGGTTCAAGTTCTGGTTTCTACTGCACCGGCTACTGCTCAACTTCACTCAAGTATTCAGAGTTAATTAATTGGTTAAGAATAGCCTTCTGCAAATTATTATAATTGTACTGTAAATCATAAATGAAACAACAAAGTTTGTATAAACTATACTATTTAGTTTGGTGCCCCTTTAGAATAGAGACGATCATACTGGACTTAAAAGACTACTTGTGAACTAAATGTCGATCCTCTAACATCAAAGGA ************************************************((((((...(((((.(((((((...(((...((((....))))...)))...)))).......))).)))))..))))))((((((((.(((((....))))).))))))))(((.((..((((((.......))))))...)).)))..************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	SRR553488 RT_0-2 hours eggs	SRR553487 NRT_0-2 hours eggs	SRR553485 Chicharo_3 day-old ovaries	SRR618934 dsim w501 ovaries	SRR553486 Makindu_3 day-old ovaries
.........................................................................................................................................................................AGCCCCTTTAGAATAGA................................................................	17	1	1	2.00	2	1	1	0	0	0
.......................................................................................................................................................................GGAGCCCCTTTAGAATA..................................................................	17	1	1	1.00	1	0	1	0	0	0
......................................................................................................................................................................CGGGGCCCCTTTAGAATAG.................................................................	19	2	1	1.00	1	1	0	0	0	0
........................................................................................................................................................................GAGCCCCTTTAGAATAG.................................................................	17	1	1	1.00	1	0	0	1	0	0
........................................................................................................................................................................GGGCCCCTTTAGAAT...................................................................	15	1	3	0.33	1	1	0	0	0	0
..........................................................................................................................................................................GCCCCTTTAGAATAGAA...............................................................	17	1	4	0.25	1	1	0	0	0	0
.........................................................................................................................................................................GGCCCCTTTAGAATA..................................................................	15	1	10	0.20	2	2	0	0	0	0
.........................................................................................................................................................................AGCCCCTTTAGAATAGAA...............................................................	18	2	10	0.10	1	1	0	0	0	0
....................................................................................................................................................................TTCAGAGCCCCTTTAGAATA..................................................................	20	3	14	0.07	1	1	0	0	0	0
..........................................................................................................................................................................GGCCCTTTAGAGTAGAG...............................................................	17	2	16	0.06	1	0	0	1	0	0
.........................................................................................................................................................................TAGCCCTTTAGAATAGA................................................................	17	2	17	0.06	1	1	0	0	0	0
.......................................................................................................................................................................AGAGCCCCTTTAGAATA..................................................................	17	2	17	0.06	1	0	1	0	0	0
..........................................................................................................................................................................GGCCCTTTAGAGTAGA................................................................	16	2	20	0.05	1	0	1	0	0	0
.......GGAGCAACTTCTGGTTTCT................................................................................................................................................................................................................................	19	3	20	0.05	1	1	0	0	0	0
.............................................................................................................................................................................CCCTTAGAATAGAGAAG............................................................	17	2	20	0.05	1	0	0	0	1	0
.......GGAGCAAGTTCTGGTT...................................................................................................................................................................................................................................	16	2	20	0.05	1	0	0	0	0	1
......................................................................................................................................................................CGGGGCCCCCTTAGAATA..................................................................	18	3	20	0.05	1	1	0	0	0	0
...............................................................................................................................................................................CTTAGAATAGAGACG............................................................	15	1	20	0.05	1	0	0	0	1	0

Species	Coordinate	ID	Alignment
droSim2	3l:8573648-8573897 +	dsi_15596	GATATGGCCAAGTTCAAGACCAAAGATGACGTGGCCGATGACGAGTTGAAGTGAGTTCA---TAAGT--CTC----A-ATTAATTA-ACCAATTCTTATCGGA----------AGA------------CGTTTAATAATATTAAC---ATGACATTTAGTATTT---------------------------------------------ACTTTG--TTGTTTCAAACATATTTGATATGATAAATCAAACCACGGGGAAATCTTATCTCTGCTAGTATGACCTGAATTTTCT-------------GATGAACACT-------TGAT---TTACAGCTAGGAGATT----GTAGTTTCCT-------
droSec2	scaffold_0:1061180-1061429 +		GATATGGCCAAGTTCAAGACCAAAGATGACGTGGCCGATGACGAGTTGAAGTGAGTTCA---TAAGT--CTC----A-ATTAATTA-ACCAATTCTTATCGGA----------TGA------------CGTTTAATAATATTAAC---ATGACATTTAGTATAT---------------------------------------------ATTTTG--TTGTTTCAAACATATTTGATATGATAAATCAAACCACGGGGAAATCTTATCTCTGTTAGAATGACCTGAATTTTCT-------------GATGAACACT-------TGAT---TTACAGCTGGGAGATT----GTAGTTTCCT-------
dm3	chr3L:8789783-8789979 +		TAATTAACCCA---------------------------------------------------------------------------------TTCTTATCGTA----------AGA------------CTTTTAATATTATTAAC---ATGACATTTAGTATATGTATATTGTGT----------------------------------TTTTTG--TTGTTTCAAACATATTTGATATGATAAATCAAACCACGGAGAAATCTTATCTCTGCTAGTATAACCTGAATTTTCT-------------GATGAACACTTTC-AAGTGAT---TTACAGCTGGGAGATT----ATAGTTTCCT-------
droEre2	scaffold_4784:5255459-5255695 -		GACATGGCCAAGTTCAAGACCAAAGATGACGTGGCCGATGATGAGTTGAAGTGAGCTTA---TTAGT--CTT----A-ATTCGTTG-ACCAATTCTTATCGGA----------AGA------------CGTTTAATAATATTAAC---ATGACATTTAGTATAC---------------------------------------------ATTT--------------------TGATGTGATAAATCAAACCACGGGGAAATCTTATCTCCACTAAAATGACCTGAATTT-CT-------------AATGAACATTTTG-AAGTGAT---TTAGAGCTGGAAGGTT----GTAGTTTCCT-------
droYak3	3L:3125082-3125334 -		GATATGGCCAAGTTCAAGACCAAAGATGACGTGGCCGATGATGAGTTGAAGTGAGTTTA---TTAGT--CTT----A-ATTAGTCT-A--AATTCTTATCGGT----------AGA------------CGTTTAATAGTATTAAC---ATGACATCTAGCATAC---------------------------------------------ATATTG--ATGTGTTAAACATATTTGATATGATAAATCAAACCACGGGGAAATCTTATCTCCACTCAAATGACCTGAATTT-CT-------------GATTAACACTTTA-AAGTGAT---TTACAGCTGGGAGATT----GTAGTTTTCT-------
droBia1	scf7180000300910:2421886-2422151 -		GACATGGCCAAGTTCAAGACCAAAGATGACGTGGCCGATGACGAGCTGAAGTGAGTTTC---GG--T--TTG----AGTTTGAGTA-GTGCCTTCTTATCTTG-------AAAAGA----------TTTATCTACTAATAGTAAC---ATGAC-TGTAGTACGC---------------------------------------------ATTTTGATATGTGTTAAGCACCCGTGAAATGATAAATCAAACAGCGAGGAAAGCTTATCGCTGTGGAAGTGACCTGAATTT-CTGAGAATTTTATG-----AG-------AAAGTGAT---TTACAGTTGTAAGATACGAAGTAGTTTATC-------
droTak1	scf7180000415356:433209-433452 -		GACATGGCCAAGTTCAAGACCAAAGATGACGTGGCCGATGACGAGTTGAAGTGAGTTTG---AAAGT--CTA------------CA-ACAAGTTCTTATC------------------------------------AATATTAAC---ATGACATTT-------------------------------------------------------CTG--ATATGTTCCACCCATGTGAAACTAAAAATCAAACCACAAGCAAAGCTTATCGCTATTGAAATGACCTGAATTT-CGAAGAATGTTATGTGATGAACAATTCAAAAGTGAATTTTTAAAA-TTTAAGATAAGAAATACTTTTAT-------
droEle1	scf7180000490564:9791-10054 +		GACATGGCCAAGTTCAAGACCAAAGATGACGTGGCCGACGACGAACTGAAGTGAGTATT---GA--G---GG----C-ATTACATC-ATAAATTCTTATCATTCCATTTCCAAAGATATTTTATAATCTAGTCACCAATATTAAC---ATGAC-TTAAGTACAT--------------------------------------------CATAT--------------------TGAAACGATAAGTCAAACCAAATCACCTGCTTATCGCTATTCAAATGACCCGAATTT-CCGGAAATATAATATGATAAGCTTTTTTAAAGT----------------GAGACT----AACATTTCTCAACGGCT
droRho1	scf7180000766314:17541-17879 +		GACATGGCCAAGTTCAAGACCAAAGATGACGTGGCCGATGACGAGTTGAAGTGAGTGTG---GA--TTTCTAAGCATGTTTAAGTA-ATATATTCTTATCGCACTTTTTCAAAAGCCATTTC--AATCTATGCACTAATATTGTCAAGTTGATAT-TAGTAAAT--AGATTGAATTTTCTTTTAAAATGGTATGATAAGAAGCAAGCTAGTTTTG--ATGTGTTAAATACATGTGAAATGATAAATCAAACCTTAACGCATACTTATCGCTATTGAAGTGACCTGAATTT-CTTGAAATATTATATGTTCAACCTTTTGAAAGTGAT---TTATAATTGTGAGATA----AGAATTTCTT-------
droFic1	scf7180000452451:95087-95368 -		GACATGGCCAAGTTCAAGACCAAAGACGATGTGGCCGATGACGAGTTGAAGTGAGTTTT---GG--G---TC----G-ATTAAGTA-ATAAGTTCTTATCGTAACACTCAAAAAGACACTTT--AAGCTGCTTACTAATATAAAC---ATGACATTAAGTACAT--------------------------------------------AATGTTG--ATGAGTTGAAGACACGTGAAGCGATAAATTACACCTCAGGGTATGCTTATCTTTTTAAAAGTGACCTGATTT-----GGAGTATTATGTGGTAAACATTTTAAAAATGAT---TTATTATTGTGAGATA----AGAATTT-TT-------
droKik1	scf7180000302474:172209-172265 -		GACATGGCCAAGTTCAAGACCAAAGAGGATGTGGCCGACGCCGAGTTGAAGTGAGTT----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droAna3	scaffold_13337:7857730-7857786 -		GACATGGCCAAGTTCAAGACCAAAGAGGATGTGGCTGATGCGGAGTTGAAGTGAGTT----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droBip1	scf7180000396541:12369-12424 -		GACATGGCCAAGTTCAAGACCAAAGAGGATGTGGCTGATGCGGAGTTGAAGTGAGT-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
dp5	XR_group8:8793059-8793110 -		GATATGGCCAAGTTCAAGACCAAAGAGGATGTGGCTGATGCAGAGTTGAAGT---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droPer2	scaffold_50:413300-413351 +		GATATGGCCAAGTTCAAGACCAAAGAGGATGTGGCCGATGCAGAGTTGAAGT---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droWil2	scf2_1100000004511:8047773-8047849 -		GATATGGCCAAATTCAAAACCAAAGAGGATGTTGCCGATGCCGAATTGAAGTGAGTTCA---CAAGG--ATG----A-GTGGATTG-A---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droVir3	scaffold_13049:6463248-6463340 +		GACATGGCCAAGTTCAAGACCAAAGAAGATGTGGCCGATGCCGAGCTAAAGTAAGTGTGCCCCAAGT--TTC----A-CACAGTTACATCAATTAGCATC---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droMoj3	scaffold_6680:4777516-4777571 +		GACATAGTCAAGTTCAAGACCAAAGAGGATGTGGCTGATGCCGAACTAAAGTATGT-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droGri2	scaffold_15110:6293865-6293932 -		GACATGGCCAAGTTCAAGACCAAAGAGGATGTCGCCGATGCAGAGCTGAAGTGAGTGCA---AA--T------------------------------------------------------------------------------------------------------------------------------------------------CTTG-----------------------------------------------------------------------------------------------------------------------------------------------AT-------

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droSim2

GATATGGCCAAGTTCAAGACCAAAGATGACGTGGCCGATGACGAGTTGAAGTGAGTTCA---TAAGT--CTC----A-ATTAATTA-ACCAATTCTTATCGGA----------AGA------------CGTTTAATAATATTAAC---ATGACATTTAGTATTT---------------------------------------------ACTTTG--TTGTTTCAAACATATTTGATATGATAAATCAAACCACGGGGAAATCTTATCTCTGCTAGTATGACCTGAATTTTCT-------------GATGAACACT-------TGAT---TTACAGCTAGGAGATT----GTAGTTTCCT-------	Size	Hit Count	Total Norm	Total	M023 Head	M024 Male-body	M025 Embryo	M053 Female-body	O001 Testis	O002 Head	SRR553485 Ovary	SRR553486 Ovary	SRR553487 Ovary	SRR553488 Ovary	SRR618934 Ovary	V044 Embryo	SRR902008 Ovary	SRR902009 Testis	SRR1275487 Male larvae	SRR1275485 Male prepupae	SRR1275483 Male prepupae
...............................................................................................................................................................................................................................................................................................TTTTCT-------------GATGAACACT-------TGAT---T....................................	21	1	4.00	4	0	0	3	0	0	0	0	0	0	0	0	1	0	0	0	0	0
...............................................................................................................................................................................................................................................................................................TTTTCT-------------GATGAACACT-------TGAT---TT...................................	22	1	2.00	2	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...................CCAAAGATGACGTGGCCGATGACGAG..................................................................................................................................................................................................................................................................................................................................	26	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0
......................................................................................................................................................................................................................G--TTGTTTCAAACATATTTGATATGATA............................................................................................................................	27	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0
.....GGCCAAGTTCAAGACCAAAGAT....................................................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0
......................AAGATGACGTGGCCGATGACGAGT.................................................................................................................................................................................................................................................................................................................................	24	1	1.00	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
...........GTTCAAGACCAAAGATGACGTGGCC...........................................................................................................................................................................................................................................................................................................................................	25	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
GATATGGCCAAGTTCAAG.............................................................................................................................................................................................................................................................................................................................................................	18	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0
..........................TGACGTGGCCGATGACGA...................................................................................................................................................................................................................................................................................................................................	18	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0

droSec2

GATATGGCCAAGTTCAAGACCAAAGATGACGTGGCCGATGACGAGTTGAAGTGAGTTCA---TAAGT--CTC----A-ATTAATTA-ACCAATTCTTATCGGA----------TGA------------CGTTTAATAATATTAAC---ATGACATTTAGTATAT---------------------------------------------ATTTTG--TTGTTTCAAACATATTTGATATGATAAATCAAACCACGGGGAAATCTTATCTCTGTTAGAATGACCTGAATTTTCT-------------GATGAACACT-------TGAT---TTACAGCTGGGAGATT----GTAGTTTCCT-------	Size	Hit Count	Total Norm	Total	M054 Female-body	V113 Male-body	V114 Embryo	V115 Head
.......................AGATGACGTGGCCGATGACG....................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	1	0	0	0
.....................AAAGATGACGTGGCCGAT........................................................................................................................................................................................................................................................................................................................................	18	1	1.00	1	1	0	0	0
..................ACCAAAGATGACGTGGCCGA.........................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	1	0	0	0
......................AAGATGACGTGGCCGATGA......................................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	1	0	0	0
.....................AAAGATGACGTGGCCGATG.......................................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	1	0	0	0
...............................................................................................................................................................................................................................................................................................................................................GCTGGGAGATT----GTAGTTTC.........	19	1	1.00	1	0	0	1	0
........CAAGTTCAAGACCAAAGATGACGTG..............................................................................................................................................................................................................................................................................................................................................	25	1	1.00	1	0	0	1	0
......................AAGATGACGTGGCCGATGACG....................................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	1	0	0
.........................ATGACGTGGCCGATGACGAGTTGAAAA...........................................................................................................................................................................................................................................................................................................................	27	1	1.00	1	0	1	0	0

dm3

TAATTAACCCA---------------------------------------------------------------------------------TTCTTATCGTA----------AGA------------CTTTTAATATTATTAAC---ATGACATTTAGTATATGTATATTGTGT----------------------------------TTTTTG--TTGTTTCAAACATATTTGATATGATAAATCAAACCACGGAGAAATCTTATCTCTGCTAGTATAACCTGAATTTTCT-------------GATGAACACTTTC-AAGTGAT---TTACAGCTGGGAGATT----ATAGTTTCCT-------	Size	Hit Count	Total Norm	Total	GSM154618 Ovary	GSM154620 Ovary	GSM154621 Ovary	GSM154622 Ovary	GSM180328 Head	GSM180329 Mixed-body	GSM180330 Embryo	GSM180331 Embryo	GSM180332 Embryo	GSM180333 Embryo	GSM180334 Larvae	GSM180335 Disc	GSM180336 Pupae	GSM180337 S2	GSM231091 Ovary	GSM239041 Head	GSM239050 Head	GSM239051 S2	GSM239052 S2	GSM246084 Head	GSM266765 S2	GSM280082 Ovary	GSM280083 Ovary	GSM280084 Ovary	GSM280085 Testis	GSM280086 Ovary	GSM280087 S2-NP	GSM280088 S2-NP	GSM312995 Head	GSM313160 Head	GSM313161 Head	GSM313162 Head	GSM313163 Head	GSM313164 Head	GSM313165 Head	GSM343287 Embryo	GSM379050 Ovary	GSM379051 Ovary	GSM379052 Ovary	GSM379053 Ovary	GSM379054 Ovary	GSM379055 Ovary	GSM379056 Ovary	GSM379057 Ovary	GSM379058 Ovary	GSM379059 Ovary	GSM379060 Ovary	GSM379061 Ovary	GSM379062 Ovary	GSM379063 Ovary	GSM379064 Ovary	GSM379065 Ovary	GSM379066 Ovary	GSM379067 Ovary	GSM424739 S2	GSM424740 S2	GSM424741 S2	GSM467729 Female-body	GSM467730 Female-body	GSM467731 Female-body	M002 CS	M003 Male-body	M004 Male-body	M005 ML-DmD32	M006 ML-DmD32	M029 OSC	M030 OSC	M031 OSC	M032 S2-R+	M033 S2-R+	M034 S2-R+	M035 S2-R+	M036 S2-R+	M037 S2-R+	M038 S2-R+	M039 S2-R+	M048 S2-R+	OSS2 OSS	OSS6 OSS	OSS7 OSS	OSS8 OSS	S1 Head	S10 Embryo	S11 Embryo	S12 Embryo	S13 Embryo	S14 Embryo	S15 S2-GMR	S16 Kc	S17 Larvae	S18 Larvae	S19 Pupae	S2 Head	S20 Pupae	S21 Disc	S22 Head	S23 Female-body	S24 Male-body	S25 Larvae	S26 Larvae	S27 Pupae	S28 Pupae	S29 Head	S3 Male-body	S30 S2-GMR	S31 S2	S32 S2	S33 Kc	S34 Kc	S35 Kc	S4 Female-body	S5 Disc	S6 Embryo	S7 Embryo	S8 S2	S9 Embryo	SRR001337 Head	SRR001338 Head	SRR001339 Head	SRR001340 Head	SRR001341 Head	SRR001342 Head	SRR001343 Head	SRR001344 Head	SRR001345 Head	SRR001346 Head	SRR001347 Head	SRR001348 Head	SRR001349 Head	SRR001664 Head	SRR010951 Ovary	SRR010952 Ovary	SRR010953 Ovary	SRR010954 Ovary	SRR010955 Ovary	SRR010956 Ovary	SRR010957 Ovary	SRR010958 Ovary	SRR010959 Ovary	SRR010960 Ovary	SRR014268 Embryo	SRR014269 Embryo	SRR014270 Embryo	SRR014271 Embryo	SRR014272 Embryo	SRR014273 Ovary	SRR014274 Ovary	SRR014275 Ovary	SRR014276 Ovary	SRR014277 Ovary	SRR014278 Ovary	SRR014279 Ovary	SRR014280 Ovary	SRR014281 Ovary	SRR014282 Ovary	SRR023197 S2	SRR023399 S2	SRR023400 S2	SRR023402 S2	SRR023407 S2	SRR029028 S2	SRR029029 S2	SRR029030 S2	SRR029031 S2	SRR029032 S2	SRR029033 S2	SRR029608 Head	SRR029633 Head	SRR030145 gb3	SRR031692 Head	SRR031693 Head	SRR031694 Head	SRR031696 Head	SRR031697 Head	SRR031698 Head	SRR031699 Head	SRR031700 Head	SRR031701 Head	SRR031702 Head	SRR031703 Head	SRR031704 Head	SRR032092 S2	SRR032093 S2	SRR032094 S2	SRR032095 S2-NP	SRR032096 S2-NP	SRR032152 Head	SRR060643 Testis	SRR060644 Ovary	SRR060645 Testis	SRR060646 Ovary	SRR060647 Ovary	SRR060648 Ovary	SRR060649 Ovary	SRR060650 Testis	SRR060651 Ovary	SRR060652 Testis	SRR060653 Ovary	SRR065151 Ovary	SRR065152 Ovary	SRR065153 Ovary	SRR065154 Ovary	SRR065155 Ovary	SRR065800 Ovary	SRR065801 Ovary	SRR065802 Ovary	SRR065803 Ovary	SRR065804 Ovary	SRR065805 Ovary	SRR065806 Ovary	SRR065807 Ovary	SRR097865 S2-NP	SRR097866 S2-NP	SRR097867 S2-NP	SRR298536 Ovary	SRR298537 Ovary	SRR298711 Ovary	SRR298712 Ovary	SRR317110 Ovary	SRR317111 Ovary	SRR317112 Ovary	SRR317113 Ovary	SRR317114 Ovary	SRR317115 Ovary	SRR317116 Ovary	SRR317117 Ovary	SRR341115 S2	SRR341116 S2	SRR341117 S2	SRR341118 S2	SRR351332 Mixed-body	SRR351333 Mixed-body	T004 OSC	T005 CME-L1	V001 Embryo	V002 Embryo	V003 S2	V006 Female-body	V007 Head	V008 S2	V009 CME-L1	V010 ML-DmD20-C5	V011 Sg4	V012 Male-body	V013 Head	V014 DTT	V015 DreRFHV	V018 Kc167	V019 GM2	V020 S2-R+	V021 ML-DmD21	V022 ML-DmD32	V023 Head	V024 Kc167	V025 S2-R+	V026 1182-2H	V027 ML-DmD21	V028 CME-W1-Cl.8+	V029 ML-DmD9	V030 ML-DmD8	V031 GM2	V032 S1	V033 CME-W1-Cl.8+	V034 ML-DmD16-C3	V035 ML-DmD32	V036 ML-DmD20-C5	V037 S2	V038 S2	V063 Ovary	V0632 Ovary	V064 Ovary	V0642 Ovary	V065 Ovary	V0652 Ovary	V066 Ovary	V0662 Ovary	V067 S2-NP	V073 mbn2	V074 S3	V075 ML-DmBG1-C1	V076 ML-DmBG3-C2	V077 Head	V078 Head	V079 Head	V080 Head	V081 Embryo	V082 Embryo	V083 Male-body	V084 Female-body	V085 CME-W2	V086 Female-body	V088 Ovary	V0882 Ovary	V089 Ovary	V0892 Ovary	V090 Male-body	V091 fGS/OSS	V092 Male-body	V093 Male-body	V094 S2-R+	V095 S2-R+	V096 Ovary	V097 Ovary	V098 Ovary	V125 ML-DmD9	V126 CME-L1	V127 G2	V128 S3	V129 ML-DmBG1-C1	V130 ML-DmBG3-C2	V131 ML-DmD16-C3	V132 ML-DmD32	V133 Sg4	V134 ML-DmD8	V135 CME-W2	V136 Male-body	V137 Head	V138 Male-body	V139 Female-body	V140 Female-body	V141 Female-body	V142 Female-body	V144 OSC	V145 S2	V146 S1	V147 1182-2H	V148 mbn2	GSM1528798 follicle cells
.................................................................................................................................................................................................................................................................................................................................................TGGGAGATT----ATAGTTTCG........	18	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.................................................................................................................................................................................................................................................................................................................................................TGGGAGATT----ATAGTTTCT........	18	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
............................................................................................................................................................................................................................................................................................GAATTTTCT-------------GATGAACACT...................................................	19	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

droEre2

GACATGGCCAAGTTCAAGACCAAAGATGACGTGGCCGATGATGAGTTGAAGTGAGCTTA---TTAGT--CTT----A-ATTCGTTG-ACCAATTCTTATCGGA----------AGA------------CGTTTAATAATATTAAC---ATGACATTTAGTATAC---------------------------------------------ATTT--------------------TGATGTGATAAATCAAACCACGGGGAAATCTTATCTCCACTAAAATGACCTGAATTT-CT-------------AATGAACATTTTG-AAGTGAT---TTAGAGCTGGAAGGTT----GTAGTTTCCT-------	Size	Hit Count	Total Norm	Total	M055 Female-body	M057 Embryo	V060 Head	V040 Embryo	V107 Male-body	V108 Head	GSM1528801 follicle cells
.....................AAAGATGACGTGGCCGAT........................................................................................................................................................................................................................................................................................................................................	18	1	2.00	2	1	0	0	0	0	1	0
.................GACCAAAGATGACGTGGCC...........................................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	0	0	0	0	1	0	0
........................GATGACGTGGCCGATGATG....................................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	0	0	0	0	1	0	0
.........AAGTTCAAGACCAAAGATGACGTGGCC...........................................................................................................................................................................................................................................................................................................................................	27	1	1.00	1	0	0	0	0	1	0	0
....................CAAAGATGACGTGGCCGATGATG....................................................................................................................................................................................................................................................................................................................................	23	1	1.00	1	0	0	0	0	0	1	0
.....................AAAGATGACGTGGCCGATGATGAGTT................................................................................................................................................................................................................................................................................................................................	26	1	1.00	1	0	0	0	0	0	1	0
......................AAGATGACGTGGCCGATGAT.....................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	0	0	0	0	1	0
..........................TGACGTGGCCGATGATGAGTTGAAAAA..........................................................................................................................................................................................................................................................................................................................	27	1	1.00	1	0	0	0	0	1	0	0
.........AAGTTCAAGACCAAAGAT....................................................................................................................................................................................................................................................................................................................................................	18	1	1.00	1	0	0	0	0	0	1	0
.....................AAAGATGACGTGGCCGATGATGAG..................................................................................................................................................................................................................................................................................................................................	24	1	1.00	1	0	0	0	0	1	0	0
....TGGCCAAGTTCAAGACCAA........................................................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	0	0	0	0	0	1	0
.........................ATGACGTGGCCGATGATGAGTTGAAA............................................................................................................................................................................................................................................................................................................................	26	1	1.00	1	0	0	0	0	0	1	0
.............................................................................................................................................................................................................................................................................................................................................GAGCTGGAAGGTT----GTAGTTTCCT-------	23	1	1.00	1	1	0	0	0	0	0	0
.....................AAAGATGACGTGGCCGATGATGAGT.................................................................................................................................................................................................................................................................................................................................	25	1	1.00	1	0	0	0	0	1	0	0
....................CAAAGATGACGTGGCCGAT........................................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	0	0	0	0	1	0	0
......................AAGATGACGTGGCCGATGATG....................................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	1	0	0	0	0	0	0
.....................AAAGATGACGTGGCCGATG.......................................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	1	0	0	0	0	0	0
...............................TGGCCGATGATGAGTTGAAAAA..........................................................................................................................................................................................................................................................................................................................	22	5	0.20	1	1	0	0	0	0	0	0

droYak3

GATATGGCCAAGTTCAAGACCAAAGATGACGTGGCCGATGATGAGTTGAAGTGAGTTTA---TTAGT--CTT----A-ATTAGTCT-A--AATTCTTATCGGT----------AGA------------CGTTTAATAGTATTAAC---ATGACATCTAGCATAC---------------------------------------------ATATTG--ATGTGTTAAACATATTTGATATGATAAATCAAACCACGGGGAAATCTTATCTCCACTCAAATGACCTGAATTT-CT-------------GATTAACACTTTA-AAGTGAT---TTACAGCTGGGAGATT----GTAGTTTTCT-------	Size	Hit Count	Total Norm	Total	M026 Head	M043 Female-body	M056 Embryo	V046 Embryo	V052 Head	V058 Head	V120 Male-body	SRR1275488 Male larvae	SRR1275486 Male prepupae	SRR1275484 Male prepupae	GSM1528802 follicle cells
..............................GTGGCCGATGATGAGTTGAA.............................................................................................................................................................................................................................................................................................................................	20	1	2.00	2	0	0	0	0	0	0	2	0	0	0	0
..TATGGCCAAGTTCAAGACC..........................................................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0
........................GATGACGTGGCCGATGA......................................................................................................................................................................................................................................................................................................................................	17	1	1.00	1	0	0	0	0	0	1	0	0	0	0	0
.......................AGATGACGTGGCCGATGATGAGT.................................................................................................................................................................................................................................................................................................................................	23	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
................................GGCCGATGATGAGTTGAA.............................................................................................................................................................................................................................................................................................................................	18	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
.........................ATGACGTGGCCGATGATGA...................................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
..............................GTGGCCGATGATGAGTTGAAGAA..........................................................................................................................................................................................................................................................................................................................	23	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
....................CAAAGATGACGTGGCCGATGAT.....................................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
...............................TGGCCGATGATGAGTTGAAG............................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0
.....................AAAGATGACGTGGCCGATGATG....................................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
................................GGCCGATGATGAGTTGAAG............................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
...............................TGGCCGATGATGAGTTGAAGA...........................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
...........GTTCAAGACCAAAGATGACGTG..............................................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
......................AAGATGACGTGGCCGATGATGAGTT................................................................................................................................................................................................................................................................................................................................	25	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
................................GGCCGATGATGAGTTGAAGAA..........................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0

droBia1

GACATGGCCAAGTTCAAGACCAAAGATGACGTGGCCGATGACGAGCTGAAGTGAGTTTC---GG--T--TTG----AGTTTGAGTA-GTGCCTTCTTATCTTG-------AAAAGA----------TTTATCTACTAATAGTAAC---ATGAC-TGTAGTACGC---------------------------------------------ATTTTGATATGTGTTAAGCACCCGTGAAATGATAAATCAAACAGCGAGGAAAGCTTATCGCTGTGGAAGTGACCTGAATTT-CTGAGAATTTTATG-----AG-------AAAGTGAT---TTACAGTTGTAAGATACGAAGTAGTTTATC-------	Size	Hit Count	Total Norm	Total
No Reads

droTak1

GACATGGCCAAGTTCAAGACCAAAGATGACGTGGCCGATGACGAGTTGAAGTGAGTTTG---AAAGT--CTA------------CA-ACAAGTTCTTATC------------------------------------AATATTAAC---ATGACATTT-------------------------------------------------------CTG--ATATGTTCCACCCATGTGAAACTAAAAATCAAACCACAAGCAAAGCTTATCGCTATTGAAATGACCTGAATTT-CGAAGAATGTTATGTGATGAACAATTCAAAAGTGAATTTTTAAAA-TTTAAGATAAGAAATACTTTTAT-------	Size	Hit Count	Total Norm	Total
No Reads

droEle1

GACATGGCCAAGTTCAAGACCAAAGATGACGTGGCCGACGACGAACTGAAGTGAGTATT---GA--G---GG----C-ATTACATC-ATAAATTCTTATCATTCCATTTCCAAAGATATTTTATAATCTAGTCACCAATATTAAC---ATGAC-TTAAGTACAT--------------------------------------------CATAT--------------------TGAAACGATAAGTCAAACCAAATCACCTGCTTATCGCTATTCAAATGACCCGAATTT-CCGGAAATATAATATGATAAGCTTTTTTAAAGT----------------GAGACT----AACATTTCTCAACGGCT	Size	Hit Count	Total Norm	Total
No Reads

droRho1

GACATGGCCAAGTTCAAGACCAAAGATGACGTGGCCGATGACGAGTTGAAGTGAGTGTG---GA--TTTCTAAGCATGTTTAAGTA-ATATATTCTTATCGCACTTTTTCAAAAGCCATTTC--AATCTATGCACTAATATTGTCAAGTTGATAT-TAGTAAAT--AGATTGAATTTTCTTTTAAAATGGTATGATAAGAAGCAAGCTAGTTTTG--ATGTGTTAAATACATGTGAAATGATAAATCAAACCTTAACGCATACTTATCGCTATTGAAGTGACCTGAATTT-CTTGAAATATTATATGTTCAACCTTTTGAAAGTGAT---TTATAATTGTGAGATA----AGAATTTCTT-------	Size	Hit Count	Total Norm	Total
No Reads

droFic1

GACATGGCCAAGTTCAAGACCAAAGACGATGTGGCCGATGACGAGTTGAAGTGAGTTTT---GG--G---TC----G-ATTAAGTA-ATAAGTTCTTATCGTAACACTCAAAAAGACACTTT--AAGCTGCTTACTAATATAAAC---ATGACATTAAGTACAT--------------------------------------------AATGTTG--ATGAGTTGAAGACACGTGAAGCGATAAATTACACCTCAGGGTATGCTTATCTTTTTAAAAGTGACCTGATTT-----GGAGTATTATGTGGTAAACATTTTAAAAATGAT---TTATTATTGTGAGATA----AGAATTT-TT-------	Size	Hit Count	Total Norm	Total
No Reads

droKik1

GACATGGCCAAGTTCAAGACCAAAGAGGATGTGGCCGACGCCGAGTTGAAGTGAGTT----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droAna3

GACATGGCCAAGTTCAAGACCAAAGAGGATGTGGCTGATGCGGAGTTGAAGTGAGTT----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M044 Female-body	M058 Embryo	V039 Embryo	V055 Head	V105 Male-body	V106 Head
......................AAGAGGATGTGGCTGATGCGGAGT.................................................................................................................................................................................................................................................................................................................................	24	1	3.00	3	2	0	0	0	1	0
.......................AGAGGATGTGGCTGATGCGGA...................................................................................................................................................................................................................................................................................................................................	21	1	2.00	2	1	0	0	0	1	0
..............................GTGGCTGATGCGGAGTTGAAA............................................................................................................................................................................................................................................................................................................................	21	1	2.00	2	0	0	0	0	0	2
..........................GGATGTGGCTGATGCGGAGTTGAAACA..........................................................................................................................................................................................................................................................................................................................	27	1	2.00	2	2	0	0	0	0	0
.........................AGGATGTGGCTGATGCGGAGT.................................................................................................................................................................................................................................................................................................................................	21	1	2.00	2	2	0	0	0	0	0
.................GACCAAAGAGGATGTGGCTGATGCGGA...................................................................................................................................................................................................................................................................................................................................	27	1	1.00	1	0	0	0	0	1	0
...........................GATGTGGCTGATGCGGAGTTGAAACA..........................................................................................................................................................................................................................................................................................................................	26	1	1.00	1	0	0	0	0	1	0
........................GAGGATGTGGCTGATGCGGAGTT................................................................................................................................................................................................................................................................................................................................	23	1	1.00	1	1	0	0	0	0	0
......................AAGAGGATGTGGCTGATGCGGAG..................................................................................................................................................................................................................................................................................................................................	23	1	1.00	1	1	0	0	0	0	0
........................GAGGATGTGGCTGATGCGGAGTTGA..............................................................................................................................................................................................................................................................................................................................	25	1	1.00	1	1	0	0	0	0	0
.........................AGGATGTGGCTGATGCGG....................................................................................................................................................................................................................................................................................................................................	18	1	1.00	1	1	0	0	0	0	0
.....................AAAGAGGATGTGGCTGATG.......................................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	0	0	0	0	1	0
................AGACCAAAGAGGATGTGGCTGATGCGG....................................................................................................................................................................................................................................................................................................................................	27	1	1.00	1	1	0	0	0	0	0
........................GAGGATGTGGCTGATGCGGAGT.................................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	0	0	1	0
..................ACCAAAGAGGATGTGGCTGATGCGGA...................................................................................................................................................................................................................................................................................................................................	26	1	1.00	1	0	0	0	0	1	0
............................ATGTGGCTGATGCGGAGTTGAA.............................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	1	0	0	0	0	0
.................GACCAAAGAGGATGTGGCTGATGC......................................................................................................................................................................................................................................................................................................................................	24	1	1.00	1	0	0	0	0	1	0
............TTCAAGACCAAAGAGGATGTGGCTG..........................................................................................................................................................................................................................................................................................................................................	25	1	1.00	1	1	0	0	0	0	0
.....................AAAGAGGATGTGGCTGATGC......................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	1	0	0	0	0	0
...........................GATGTGGCTGATGCGGAGTTGAAA............................................................................................................................................................................................................................................................................................................................	24	1	1.00	1	1	0	0	0	0	0
......................AAGAGGATGTGGCTGATGCGGAGTT................................................................................................................................................................................................................................................................................................................................	25	1	1.00	1	1	0	0	0	0	0
.....................AAAGAGGATGTGGCTGATGCGGA...................................................................................................................................................................................................................................................................................................................................	23	1	1.00	1	1	0	0	0	0	0
...........................GATGTGGCTGATGCGGAGTT................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	1	0	0	0	0	0

droBip1

GACATGGCCAAGTTCAAGACCAAAGAGGATGTGGCTGATGCGGAGTTGAAGTGAGT-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

dp5

GATATGGCCAAGTTCAAGACCAAAGAGGATGTGGCTGATGCAGAGTTGAAGT---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
...........GTTCAAGACCAAAGAGGATGTGGCT...........................................................................................................................................................................................................................................................................................................................................	25	1	1.00	1	0	0	0	0	0	0	1	0	0	0
...............AAGACCAAAGAGGATGTGGCT...........................................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	1	0	0	0	0	0	0	0	0
...........GTTCAAGACCAAAGAGGAC.................................................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	0	0	1	0	0	0	0	0	0	0
......GCCAAGTTCAAGACCAAAGAG....................................................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	1	0	0	0	0	0	0	0	0
...............AAGACCAAAGAGGATGTGGCC...........................................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	1	0	0	0	0	0	0	0	0
................AGACCAAAGAGGATGTGGCTGATG.......................................................................................................................................................................................................................................................................................................................................	24	1	1.00	1	0	1	0	0	0	0	0	0	0	0
..........AGTTCAAGACCAAAGAGGATGTGGCC...........................................................................................................................................................................................................................................................................................................................................	26	1	1.00	1	0	0	0	1	0	0	0	0	0	0
GATATGGCCAAGTTCAAGACC..........................................................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	1	0	0	0	0	0	0	0	0
..........AGTTCAAGACCAAAGAGGATGTGGAC...........................................................................................................................................................................................................................................................................................................................................	26	1	1.00	1	0	0	0	1	0	0	0	0	0	0

droPer2

GATATGGCCAAGTTCAAGACCAAAGAGGATGTGGCCGATGCAGAGTTGAAGT---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
...........................GATGTGGCCGATGCAGAGTTGAA.............................................................................................................................................................................................................................................................................................................................	23	1	3.00	3	0	0	0	0	0	3
........................GAGGATGTGGCCGATGCAGAG..................................................................................................................................................................................................................................................................................................................................	21	1	3.00	3	0	0	0	0	0	3
......................AAGAGGATGTGGCCGATGC......................................................................................................................................................................................................................................................................................................................................	19	1	2.00	2	0	2	0	0	0	0
.......................AGAGGATGTGGCCGATGCA.....................................................................................................................................................................................................................................................................................................................................	19	1	2.00	2	0	1	0	0	0	1
.....................AAAGAGGATGTGGCCGATGCA.....................................................................................................................................................................................................................................................................................................................................	21	1	2.00	2	0	0	0	0	0	2
......................AAGAGGATGTGGCCGATGCAG....................................................................................................................................................................................................................................................................................................................................	21	1	2.00	2	0	0	0	0	0	2
....................CAAAGAGGATGTGGCCGATGCAG....................................................................................................................................................................................................................................................................................................................................	23	1	1.00	1	0	0	0	0	0	1
........................GAGGATGTGGCCGATGCAGAGTTG...............................................................................................................................................................................................................................................................................................................................	24	1	1.00	1	0	0	0	0	0	1
..........................GGATGTGGCCGATGCAGAGTTG...............................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	0	0	0	1
......................AAGAGGATGTGGCCGATGCA.....................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	1	0	0	0	0
.............................TGTGGCCGATGCAGAGTTG...............................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	0	0	0	0	0	1
................AGACCAAAGAGGATGTGGCC...........................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	0	0	0	0	1
......................AAGAGGATGTGGCCGATGCAGAGTTGAA.............................................................................................................................................................................................................................................................................................................................	28	1	1.00	1	0	0	0	0	0	1
.....................AAAGAGGATGTGGCCGATGCAGAGTT................................................................................................................................................................................................................................................................................................................................	26	1	1.00	1	0	0	0	0	0	1
...............AAGACCAAAGAGGATGTGGCC...........................................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	1	0	0	0	0
...........................GATGTGGCCGATGCAGAGTTGAAA............................................................................................................................................................................................................................................................................................................................	24	1	1.00	1	0	0	0	0	0	1
....................CAAAGAGGATGTGGCCGATGC......................................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	1
.....................AAAGAGGATGTGGCCGATG.......................................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	0	0	0	0	0	1
.....................AAAGAGGATGTGGCCGATGCAG....................................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	0	0	0	1
......................AAGAGGATGTGGCCGATGCAGAGTTGA..............................................................................................................................................................................................................................................................................................................................	27	1	1.00	1	0	0	0	0	0	1
............................ATGTGGCCGATGCAGAGTTGAA.............................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	1	0	0	0	0
.....................AAAGAGGATGTGGCCGATGCAGAGT.................................................................................................................................................................................................................................................................................................................................	25	1	1.00	1	0	0	0	0	0	1
........................GAGGATGTGGCCGATGCAG....................................................................................................................................................................................................................................................................................................................................	19	2	0.50	1	0	0	0	0	0	1

droWil2

GATATGGCCAAATTCAAAACCAAAGAGGATGTTGCCGATGCCGAATTGAAGTGAGTTCA---CAAGG--ATG----A-GTGGATTG-A---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
......................AAGAGGATGTTGCCGATGCCGAATTG...............................................................................................................................................................................................................................................................................................................................	26	1	3.00	3	3	0	0	0	0
......................AAGAGGATGTTGCCGATGCCGAA..................................................................................................................................................................................................................................................................................................................................	23	1	3.00	3	0	1	1	0	1
......................AAGAGGATGTTGCCGATGCCG....................................................................................................................................................................................................................................................................................................................................	21	1	2.00	2	0	2	0	0	0
.....................AAAGAGGATGTTGCCGATGCCG....................................................................................................................................................................................................................................................................................................................................	22	1	2.00	2	0	0	2	0	0
.....................AAAGAGGATGTTGCCGATGCCGAAT.................................................................................................................................................................................................................................................................................................................................	25	1	1.00	1	0	0	1	0	0
......................AAGAGGATGTTGCCGATGCCGAATT................................................................................................................................................................................................................................................................................................................................	25	1	1.00	1	0	0	1	0	0
.......................AGAGGATGTTGCCGATGCCG....................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	0	1	0	0
.....................AAAGAGGATGTTGCCGATGCCGA...................................................................................................................................................................................................................................................................................................................................	23	1	1.00	1	0	0	1	0	0
............................ATGTTGCCGATGCCGAATTGAA.............................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	1	0	0	0
....................CAAAGAGGATGTTGCCGATGCC.....................................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	1	0	0
......................AAGAGGATGTTGCCGATGCCGA...................................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	1	0	0	0
..............................GTTGCCGATGCCGAATTGAAGC...........................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	1	0	0

droVir3

GACATGGCCAAGTTCAAGACCAAAGAAGATGTGGCCGATGCCGAGCTAAAGTAAGTGTGCCCCAAGT--TTC----A-CACAGTTACATCAATTAGCATC---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M027 Male-body	M028 Head	M047 Female-body	M061 Embryo	SRR060654 Ovary	SRR060655 Testis	SRR060656 Ovary	SRR060657 Testis	SRR060658 Ovary	SRR060659 Testis	SRR060660 Ovary	SRR060661 Embryo	SRR060662 Embryo	SRR060663 Embryo	SRR060664 Male-body	SRR060665 Female-body	SRR060666 Male-body	SRR060667 Female-body	SRR060668 Male-body	SRR060669 Female-body	SRR060670 Testis	SRR060671 Male-body	SRR060672 Female-body	SRR060673 Ovary	SRR060674 Ovary	SRR060675 Ovary	SRR060676 Ovary	SRR060677 Ovary	SRR060678 Testis	SRR060679 Testis	SRR060680 Testis	SRR060681 Testis	SRR060682 Embryo	SRR060683 Testis	SRR060684 Embryo	SRR060685 Embryo	SRR060686 Embryo	SRR060687 Embryo	SRR060688 Ovary	SRR060689 Testis	SRR1106712 embryo_0-2h	SRR1106713 embryo_0-2h	SRR1106714 embryo_2-4h	SRR1106715 embryo_4-6h	SRR1106716 embryo_4-6h	SRR1106717 embryo_6-8h	SRR1106718 embryo_6-8h	SRR1106719 embryo_8-10h	SRR1106720 embryo_8-10h	SRR1106721 embryo_10-12h	SRR1106722 embryo_10-12h	SRR1106723 embryo_12-14h	SRR1106724 embryo_12-14h	SRR1106725 embryo_14-16h	SRR1106726 embryo_14-16h	SRR1106727 larvae	SRR1106728 larvae	SRR1106729 mixed whole adult body	SRR1106730 embryo_16-30h	V047 Embryo	V053 Head	V116 Male-body	GSM1528803 follicle cells
..........AGTTCAAGACCAAAGAAGATGTGGCCG..........................................................................................................................................................................................................................................................................................................................................	27	1	2.00	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0
............TTCAAGACCAAAGAAGATGTGGCC...........................................................................................................................................................................................................................................................................................................................................	24	1	2.00	2	0	0	1	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.................GACCAAAGAAGATGTGGCC...........................................................................................................................................................................................................................................................................................................................................	19	1	2.00	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...........................GATGTGGCCGATGCCGAGCT................................................................................................................................................................................................................................................................................................................................	20	1	2.00	2	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
................AGACCAAAGAAGATGTGGCC...........................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
................AGACCAAAGAAGATGTGGCCGATGCC.....................................................................................................................................................................................................................................................................................................................................	26	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..............CAAGACCAAAGAAGATGTGGC............................................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
......................AAGAAGATGTGGCCGATGC......................................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...........................GATGTGGCCGATGCCGAGCTAA..............................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0
.......................AGAAGATGTGGCCGATGCCGAGC.................................................................................................................................................................................................................................................................................................................................	23	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0
..............CAAGACCAAAGAAGATGTGGCCGAT........................................................................................................................................................................................................................................................................................................................................	25	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.................GACCAAAGAAGATGTGGCCGATGC......................................................................................................................................................................................................................................................................................................................................	24	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.................GACCAAAGAAGATGTGGC............................................................................................................................................................................................................................................................................................................................................	18	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..............CAAGACCAAAGAAGATGTGGCCGATGC......................................................................................................................................................................................................................................................................................................................................	27	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...........GTTCAAGACCAAAGAAGATGTGGCCG..........................................................................................................................................................................................................................................................................................................................................	26	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.....GGCCAAGTTCAAGACCAAAGAAGA..................................................................................................................................................................................................................................................................................................................................................	24	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.................GACCAAAGAAGATGTGGCCGATGCC.....................................................................................................................................................................................................................................................................................................................................	25	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
............TTCAAGACCAAAGAAGATGTGGCCG..........................................................................................................................................................................................................................................................................................................................................	25	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.......................AGAAGATGTGGCCGATGC......................................................................................................................................................................................................................................................................................................................................	18	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................AAGATGTGGCCGATGCCGAGCTAA..............................................................................................................................................................................................................................................................................................................................	24	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...............AAGACCAAAGAAGATGTGGCCG..........................................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0
..............CAAGACCAAAGAAGATGTGGCCG..........................................................................................................................................................................................................................................................................................................................................	23	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
............................ATGTGGCCGATGCCGAGCTA...............................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
......................AAGAAGATGTGGCCGATGCC.....................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

droMoj3

GACATAGTCAAGTTCAAGACCAAAGAGGATGTGGCTGATGCCGAACTAAAGTATGT-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M046 Female-body	V041 Embryo	V049 Head	V056 Head	M060 Embryo	V110 Male-body
........................GAGGATGTGGCTGATGCCGAACT................................................................................................................................................................................................................................................................................................................................	23	1	2.00	2	2	0	0	0	0	0
.....................AAAGAGGATGTGGCTGATGCCGAACTA...............................................................................................................................................................................................................................................................................................................................	27	1	1.00	1	1	0	0	0	0	0
.......................AGAGGATGTGGCTGATGCCG....................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	1	0	0	0	0	0
...........GTTCAAGACCAAAGAGGATGT...............................................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	1
......................AAGAGGATGTGGCTGATGCCG....................................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	1
..........................GGATGTGGCTGATGCCGAA..................................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	1	0	0	0	0	0
................AGACCAAAGAGGATGTGGCT...........................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	1	0	0	0	0	0
................AGACCAAAGAGGATGTGGCTGATGC......................................................................................................................................................................................................................................................................................................................................	25	1	1.00	1	0	0	0	0	0	1

droGri2

GACATGGCCAAGTTCAAGACCAAAGAGGATGTCGCCGATGCAGAGCTGAAGTGAGTGCA---AA--T------------------------------------------------------------------------------------------------------------------------------------------------CTTG-----------------------------------------------------------------------------------------------------------------------------------------------AT-------	Size	Hit Count	Total Norm	Total	M041 Female-body	V109 Male-body
No Reads

dsi_15596

3l:8573698-8573847 +

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-19.4	-19.0	-18.7