dsi_14207

CTGGCTAATCCGATCAGCTAAGCTCACTTAGAATGAAATCAAGGGCATGAGTACGCAGCTTAAAATAAACCGAATTGACGTAGGAAAAGTCACAGTCGCTCTCACACTCTAACTGACTAAGTACACACGCACGTGTGTTTATAAATAAATTCGGTTAAATAGTGTTTAATTTATAAATTTCCAGCTTGGACTCGCTGTGTGTGAGTGCGCATATAAATGTGTGTTTGTGTGTGTGCTGCAGTTCCAGTGA *********************************************************************...((.....((....(((...((.....))...)))....)).....))*******************************************************************************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M023 head	M025 embryo	M053 female body	O002 Head	SRR553485 Chicharo_3 day-old ovaries	SRR618934 dsim w501 ovaries	SRR553486 Makindu_3 day-old ovaries	M024 male body	GSM343915 embryo	SRR553487 NRT_0-2 hours eggs
....................................................................................................CTCACACTCTAACTGACTAAG.................................................................................................................................	21	0	1	2.00	2	1	0	0	1	0	0	0	0	0	0
...............................AATGAAATCAAGGGCATGAGTACGC..................................................................................................................................................................................................	25	0	1	1.00	1	0	1	0	0	0	0	0	0	0	0
................................................................................................CGCTCTCACACTCTAACTGAC.....................................................................................................................................	21	0	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.......................................................................GAATTGACGTAGGAAAAGT................................................................................................................................................................	19	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0
..................................................................................................................................................................................................................................GTGTGTGTGCTGCAGTTCCAGT..	22	0	1	1.00	1	0	0	1	0	0	0	0	0	0	0
.................................................................................................................................................................................................................................TGTGTGTGTGCTGTAGTTCCA....	21	1	2	0.50	1	0	0	0	0	1	0	0	0	0	0
.......................................................................................................................................................................................GCTTGGACTCGTTGTCTG.................................................	18	2	3	0.33	1	0	0	0	0	0	1	0	0	0	0
..............................................................................................................................................................................ATACTTCCAGCTTGGACT..........................................................	18	2	7	0.14	1	0	0	0	0	0	0	0	1	0	0
...............................................................................................................................................................................CATTTCCAGATTGAACTCGC.......................................................	20	3	9	0.11	1	0	0	0	0	0	0	1	0	0	0
......................................................................................................................................................................................TGCTTGGACTCCCTGTGGGT................................................	20	3	12	0.08	1	0	0	0	0	0	0	0	0	1	0
......................................................................................................................................................................................GGCTTGGACTCGTTGTGT..................................................	18	2	16	0.06	1	0	0	0	0	0	1	0	0	0	0
.............................................................................................................................................................................................................................TGATTGTGTGTGTTCTGGAGT........	21	3	17	0.06	1	0	0	0	0	0	0	0	0	0	1
...........................................................................................................TCTAACGGACTAAGGA...............................................................................................................................	16	2	20	0.05	1	0	0	0	0	0	0	1	0	0	0
...........................................................................................................................................................................................................................TGTGTTTGTGTGTGTGC..............	17	0	20	0.05	1	0	0	0	0	0	1	0	0	0	0
..............................................................................................................................................AAATAAATTCGGGTA.............................................................................................	15	1	20	0.05	1	0	0	0	0	1	0	0	0	0	0

GACCGATTAGGCTAGTCGATTCGAGTGAATCTTACTTTAGTTCCCGTACTCATGCGTCGAATTTTATTTGGCTTAACTGCATCCTTTTCAGTGTCAGCGAGAGTGTGAGATTGACTGATTCATGTGTGCGTGCACACAAATATTTATTTAAGCCAATTTATCACAAATTAAATATTTAAAGGTCGAACCTGAGCGACACACACTCACGCGTATATTTACACACAAACACACACACGACGTCAAGGTCACT *******************************************************************************************************************************...((.....((....(((...((.....))...)))....)).....))*********************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M024 male body	O002 Head	M023 head	SRR618934 dsim w501 ovaries	SRR553488 RT_0-2 hours eggs	SRR553485 Chicharo_3 day-old ovaries	SRR553486 Makindu_3 day-old ovaries
.................................................................................................CGAGAGTGTGAGATTGACT......................................................................................................................................	19	0	1	1.00	1	0	1	0	0	0	0	0
..........................................................................................................................................................................................................................CACACAAACACACACACGACGTCAAGG.....	27	0	1	1.00	1	0	1	0	0	0	0	0
...............................................................................................AGCGAGAGTGTGAGATTG.........................................................................................................................................	18	0	1	1.00	1	0	0	1	0	0	0	0
..............................................................................................CAGCGAGAGTGTGAGATTGAC.......................................................................................................................................	21	0	1	1.00	1	1	0	0	0	0	0	0
...................................................................................CTTTTCAGTGTCAACGAGAGT..................................................................................................................................................	21	1	1	1.00	1	1	0	0	0	0	0	0
............................................................................................................................................................................TATTTAAAGGTCGAAC..............................................................	16	0	4	0.25	1	0	0	0	1	0	0	0
.........................................................................................................TGAGATTGGCTGATTCG................................................................................................................................	17	2	7	0.14	1	0	0	0	0	1	0	0
.....................................................................................................................................................................................................................................ACACAGGACGTCAAG......	15	1	18	0.06	1	0	0	0	0	0	1	0
...............TCAATTCGAGTGAAT............................................................................................................................................................................................................................	15	1	20	0.05	1	0	0	0	0	0	0	1

Species	Coordinate	ID	Alignment
droSim2	2r:16001611-16001860 +	dsi_14207	CTG--------GCTAATCCGA-----TCAGCT---AAGC-TCACTTAGA--ATGAAATCAAGGGCATGAGTAC-GCAGCTTAAAATAAAC-----CGAATTGACG-TA------------G---------------------G--------------------------------------AAAA---------------------GTCACAGTCGCTCT--------------------------------------------------C----------------ACACTCTAACTG---ACTAAGTACACACGCACGTG-----------------TG------------------------------------TTTATAAATAAATTCGGTTAAATAGTGT---------------------TTAATTTATAA----ATTTCCAGCTTGGACTCG--CTGTGTGTGAGTGCGCAT--------AT-AAAT---------------------------GTG--------------------------TGTTTGTGTGTGTG-----------------------------------------------------CTGCAGTTCCAGTGA
droSec2	scaffold_376:5527-5741 +		CTG--------GCTAATCCGA-----TCAGCT---AAGC-TCACTTAGA--ATGAAATCAAGGGCATGAGTAC-GCAGCTTAAAATAAAC-----CGAATTGACG-TA------------G---------------------G--------------------------------------AAAA---------------------GTCACAGTCGCTCT--------------------------------------------------C----------------ACACTCTAACTG---ACTAAGTACACACGCACGTG-----------------TG------------------------------------TTTATAAATAAATTCGGTTAAATAGTGT---------------------TTAAGT-----------------------------------------GCGCAT--------AT-AAAT-G--------------------------TG--------------------------TGTTTGTGTGTGTG-----------------------------------------------------CTGCAGTTCCAGTGA
dm3	chr2R:15350387-15350650 +		CTG--------GCTAATCCGA-----TCAGCT---AAGC-TCACTTAGA--ATGAAATCAAGGGCATGAGTAC-GCAGCTTAAAATAAAC-----CGAATTGACG-TA------------G---------------------G--------------------------------------AAAA---------------------GTCACAGTCGCTCT--------------------------------------------------C----------------ACACTCTAACTG---ACTAAGTACACACGCACGTG-----------------TG------------------------------------TTTATAAATAAATTCGGTTAAATAGTGT---------------------TTAATTTATAA----ATTTCCAGCTTGGACTCG--CTGTGTATGAGTGCGCAT--------AT-AAAT-GTG----TGT--TTG--TGTGT--GTTTG--------------------------TGTG--TGTGTGTG-----------------------------------------------------TTGCAGTTCCAGTGA
droEre2	scaffold_4845:9548971-9549236 +		CTG--------GCTAATCCGA-----TCAGCT---AAGC-TCACTTAGA--ATGAAATCAAGGGCATGAGTAC-GCAGCTTAAAATAAAC-----CGAATTGACG-TA------------G---------------------A--------------------------------------AAAA---------------------GTCACAGTCGCTCT--------------------------------------------------C----------------ACACTCTAACTA---ACTAAGTACACACGCACGTG-----------------TG------------------------------------CTTATAAATAAATTCGGTTAAATAGTGT---------------------TTAATTTATAA----ATTTCCAGCTTGGACTCTCGTTGTGTGTTAGTGAGCAT--------AT-A---TGTA----TATATCTA--TGTGT--GGTTG--------------------------TGTG--CGTGTGTG-----------------------------------------------------TTGCAGTTCCAGTGA
droYak3	2R:13825644-13825907 -		CTG--------ACTAATCCGA-----TCAGCT---AAGC-TCACTTAGA--ATGAAATCAAGGGCATGAGTAC-GCAGCTTAAAATAAAC-----CAAATTGACG-TA------------G---------------------G--------------------------------------AAAA---------------------GTCACAGTCGCTCT--------------------------------------------------C--------------ACACTCTCCAACTG---ACTAAGTACACACGCACGTG-----------------TG------------------------------------TTTATAAATAAATTCGGTTAAATAGTGT---------------------TTAATTTATAA----ATTTCCAGCTTGGACTCTCGCTGTATGTTAGTGCCCAT--------CT-ACAT-ATA------T--CTGTGTGTA------TG--------------------------TGTG--CGTGTGTG-----------------------------------------------------TTGCAGTTCCAGTGA
droEug1	scf7180000409474:2605278-2605520 -		C------------TAATCTGA-----TCAGCG---G--AG-AGCTTAGA--ATGAAATCAAGGGCATGAGTAC-GCAGCTTAAAATAATT-----CAAATTGACG-TA------------G---------------------G--------------------------------------AAAA---------------------GTCACAGTCACAGT------T--ACA------AACGT-------A--------------T----C----------------ACTCTCTAAATA---ACTAAGTACACACGCACGTG-----------------TG------------------------------------TTTATAAATAAATTTGGTTAACTAGTGT---------------------TTAATTTATAA----ATTTCCATTCTGAT---------------------------------------TGTA----TGT---GGTGTGTGT--GTCTG--------------------------TGTT--TGTGTGTG-----------------------------------------------------TTGCAGTTCCAGTGA
droBia1	scf7180000302143:2839631-2839864 +		CTG--------GCTAATCTGA-----TCAGCAGCG---CG-AGCTTAGA--ATGA-ATCAAGGGCATGAGTAC-GCAGCTTAAAATAAGC-----CAAATTGACG-TA------------G---------------------G--------------------------------------AAAA---------------------GTCACAGTCGCAGT------C--ACAGTTCCACTCGCA----------------------------------------GGCCCTCTCTAAGTA---GCTGGGTACACAGGCACGTG-----------------TG------------------------------------TTTGTAAATAAATTCGGTTAAATAGTGT---------------------TTAATTTGCGA----ATCTCCAGCCA-------------------------------------------------------C--------------TG--------------------------TGCC--TGTGTGTG-----------------------------------------------------TTGCAGTTCCAGTGA
droTak1	scf7180000415910:157492-157745 -		CTG--------GCTAATCTGA-----TCAG---CG---AG-AGCTTAGA--ATGAAATCAAGGGCATGAGTAC-GCAGCTTAAAATAAGC-----CAAATTGACG-TA------------G---------------------G--------------------------------------AAAA---------------------GTCACAGTTACGGTCACAGTT--ACA------GTCGCAGCTCGCA----------------------------------GCACTCTCTAAATA---ACTAAGTACACACGCACGTG-----------------TG------------------------------------TTTATAAATAAATTCGGTTAAATAGTGT---------------------TTAATTTATAA----ATTTCCAGCTTG-------------------------------------------TG------T--CTGTGTCTGT--GCCTG--------------------------TGTT--TGTGTGTG-----------------------------------------------------TTGCAGTTCCAGTGA
droEle1	scf7180000491201:1798396-1798624 +		CTG--------GCTAATCTGA-----TCAA---CG---GG-AGCTTAGA--ATGAAATCAAGGGCATGAGTAC-GCAGCTTAAAATAAAC-----CGAATTGACG-TA------------G------------------------------------------------------------AAAA---------------------GGCACAGTCACAAT------C--ACA------GTCG----------------------------C----------------ACTCTCTAAAT----ACTAAGTACACACGCACGTG-----------------TG------------------------------------TTTATAAATAAATTCGGTTAAATAGTGT---------------------TTAATTTATAA----ATTTCCAGCCTGGTTTGG------------------------------------------------CTGTGCATA----------------------------------------TGTGTGTG-----------------------------------------------------TTGCAGTTCCAGTGA
droRho1	scf7180000780066:293630-293859 -		CTA--------GCTAATCTGA-----TCAA---CG---AG-AGCTTAGA--ATGAAATCAAGGGCATGAGTAC-GCAGCTTAAAATAAAC-----CAAATTGACG-TA------------G---------------------A--------------------------------------AAAA---------------------GTCACAGTCACAAT------C--ACA------GTCG----------------------------C----------------ACTCTCTAAAT----ACTTAGTACACACGCACGTG-----------------TG------------------------------------TTTATAAATAAATTCGGTTAAATAGTGT---------------------TTAATTTAAAA----ATTTCCAGCCTGGTTTGG------------------------------------------------CTGTGTATA----------------------------------------TGTGTGTG-----------------------------------------------------TTGCAGTTCCAGTGA
droFic1	scf7180000454039:640228-640470 +		CTG--------GCTAATCTGA-----TCAA--GCG---AG-AGCTTAGA--ATGAAATCAAGGGCATGAGTAC-GCAGCTTAAAATAAAC-----CAAATTGACG-TA------------G---------------------G--------------------------------------AAAA---------------------GTCACAGTCACAGT------TAAACA------GTCG----------------------------C----------------ACTCTCTAAGT----ACTAAGTACACACGCACGTG-----------------TG------------------------------------TTTATAAATAAATTCGGTTAACTAGTGT---------------------TTAATTTATAA----ATTTCCAGCCTGGGCTGGCA------------------------------------G------T--CTGTGTATG----TGTG--------------------------T--G--TTTGTGTA-----------------------------------------------------TTGCAATTCCAGTGA
droKik1	scf7180000302471:1609073-1609384 +		CTG--------GTTAATCTGATCTGATCAG---CGA--GC-AGCTCAGA--ATGAAATCAAGGGCATGAGTAC-GCAGCTTAAAATAAAC-----CAAATTGACG-CG------------G---------------------G--------------------------------------GAAAGGTCACGGGAACCGCGGAGCAGTCACAGTCACAAT------TT-AC--------------------------------------------------------AGTCACTAAATATGTACAAAGTACACACGCACGTG-----------------TGCGGCGGGT-------------------T---CTAT-TTTCGAAGTAAATTCGGTTAAATAGTGT---------------------TTAATTTATAA---AATTTCCAGAGTGAGCTT------------------------GGCTTATGAAAT-GTG--TGT------GTATTTGT--GTATGGGT------TTACGTACTC--A-ATATGCG--TGTGTGTG-----------------------------------------------------TTGCTGTTCCAGTGA
droAna3	scaffold_13266:9930538-9930909 -		CTGCCTTCCGAGCTAATCTGA-----TCAGC--------G-AGCTTAGA--ATGAAATCAAGGGCATGAGTAC-GCAGCTTAGAATAAGCAGGCCCCAATTGAG--T------GTGCCACAAAG------------------GGGGCGGGGAAGGGGTG-------------GTGGTGTCCAGGG---------------------GTCACGGGA-ATTT--------------------------------------------------C----------------AGTCTCTAAAC----GCAAAACACACACGCACGTG-----------------TCAGCTGGGTCTCGGGATCGGGTCTCTGGTCTGCTCGATTTAAAAATAAATTCGATTAAATAGTGTTTCTTTTCCTTTATTTATTTTTTAATTTATGGATGAATTTCAAAG------------------------CCCAGCTGAGCT--------TGTGTGTGT------GTGTGTGT--GTGTGTGTTTGTTGTTCCGCGTATAAGTGTATCTGTGTGAGTGTG-----------------------------------------------------TTGCAGTTCCAG--A
droBip1	scf7180000396427:1072424-1072750 -		CAA--------GCTAATCTGA-----TCAG---TG---CG-AGCTTAGA--ATGAAATCAAGGGCATGAGTAC-GCAGCTTAGAATAAGC----CCCAATTGAGC-TC------------CAAGTGTGTGGTGGTGGCTCGGAGGGCGGTGGTGGGGTGTGCAACTGTGTGTGTGGCGGCCAGGG---------------------GTCACGGG-------------------------------------------------------------------------AATTTCTAAAC----GCAAAATACACACGCACGTGTGTGCCGTCG---------------TT-------------------T---CTCG-ATTAAAAATAAATTCATTTAAATAGTGTTGCTTT-----------TTTTTTAATTTATGG----ATAA--A-------------------------GCCCAG--------CT-GAGT-GTG----T------------GT--GTGTGTGTTTGTTGTTCCGTGTTT--GTGTATGTG--TGTGTGTG-----------------------------------------------------TTGCAGTTCCAGT--
dp5	3:6503962-6504233 -		ATA--------GGTATTACGACTGTGCCGG--ACA---GG-GGCTTGAA--ATGACATCAAGGGCAACAGGACAGCGGCCAGGAGAGGGT-----GGGATTGAGGGTG------------G---------------------G-------------------------------------------------------------------CCGTCACAAA------T--------------------------------------------------------------------------------ACACACACGCACGTG-----------------CGG--------------------------A---CTTG-TTTAAAAATAAATTCGGTTAAATAGTGT---------------------TTAATTTATAA----ATTTTAAA---------------------------------------------TGTA----TGC--CAGTGTGTGT--GTGTG--------------------------CGTG--CGTGTGTGCGAGAGAGAAAGAGATTAGAGCAGAGCAAAAAAACCAACAGGGCAATAACAAGCAACAATTCCAGAGG
droPer2	scaffold_2:6723562-6723833 -		ATA--------GGTATTACGACTGTGCCGG--ACA---GG-GGCTTGAA--ATGACATCAAGGGCAACAGGACAGCGGCCAGGAGAGGGT-----GGGATTGAGGGTG------------G---------------------G-------------------------------------------------------------------CCGTCACAAA------T--------------------------------------------------------------------------------ACACACACGCACGTG-----------------CGG--------------------------A---CTTG-TTTAAAAATAAATTCGGTTAAATAGTGT---------------------TTAATTTATAA----ATTTTAAA---------------------------------------------TGTA----TGC--CAGTGTGTGT--GTGTG--------------------------CGTT--CGTGTGTGCGAGAGAGAAAGAGATTAGAGCAGAGCAAAAAAACCAACAGGGCAATAACAAGCAACAATTCCAGAGG
droWil2	scf2_1100000004514:1315997-1316261 +		ATC--------TCTTTTTCGCATA--TTAG--ACA---AATAATTTAAACCTTAAAATCAAGTGCATGCGTACGCTTATATAGAGGGAGT-----GGAAAAGTGTGTGTGAGAGAGAGACACAG------------------AGAGTGAGCAACGG--------------------------------------------------------------------------------------------GCAGGGCTGACACACACACACATACACACAAACACAAAACCTG-----------------------------TGTATAACATTGATTCGTCTA------------------------------------TTTAAAAATAAATTCAGTTAAATAGTGT---------------------TTAATTTATAA----ATTTTGTA---------------------------------------------TGTG----TCT--ATG--TATGTGTGTGTG--------------------------TGTG--CCTGAGTG------------------------------------------------------------------GG

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droSim2

CTG--------GCTAATCCGA-----TCAGCT---AAGC-TCACTTAGA--ATGAAATCAAGGGCATGAGTAC-GCAGCTTAAAATAAAC-----CGAATTGACG-TA------------G---------------------G--------------------------------------AAAA---------------------GTCACAGTCGCTCT--------------------------------------------------C----------------ACACTCTAACTG---ACTAAGTACACACGCACGTG-----------------TG------------------------------------TTTATAAATAAATTCGGTTAAATAGTGT---------------------TTAATTTATAA----ATTTCCAGCTTGGACTCG--CTGTGTGTGAGTGCGCAT--------AT-AAAT---------------------------GTG--------------------------TGTTTGTGTGTGTG-----------------------------------------------------CTGCAGTTCCAGTGA	Size	Hit Count	Total Norm	Total	M023 Head	M024 Male-body	M025 Embryo	M053 Female-body	O001 Testis	O002 Head	SRR553485 Ovary	SRR553486 Ovary	SRR553487 Ovary	SRR553488 Ovary	SRR618934 Ovary	V044 Embryo	SRR902008 Ovary	SRR902009 Testis	SRR1275487 Male larvae	SRR1275485 Male prepupae	SRR1275483 Male prepupae
..........................................................................................................................................................................................................................CT--------------------------------------------------C----------------ACACTCTAACTG---ACTAAG....................................................................................................................................................................................................................................................................................................................................	21	1	2.00	2	1	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0
................................................A--ATGAAATCAAGGGCATGAGTAC-GC............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	25	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0
......................................................................................................................................................................................................................CGCTCT--------------------------------------------------C----------------ACACTCTAACTG---AC........................................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0
................................................................................................GAATTGACG-TA------------G---------------------G--------------------------------------AAAA---------------------GT........................................................................................................................................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GTGTGTGTG-----------------------------------------------------CTGCAGTTCCAGT..	22	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0
..........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TG--------------------------TGTTTGTGTGTGTG-----------------------------------------------------C..............	17	20	0.05	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0

droSec2

CTG--------GCTAATCCGA-----TCAGCT---AAGC-TCACTTAGA--ATGAAATCAAGGGCATGAGTAC-GCAGCTTAAAATAAAC-----CGAATTGACG-TA------------G---------------------G--------------------------------------AAAA---------------------GTCACAGTCGCTCT--------------------------------------------------C----------------ACACTCTAACTG---ACTAAGTACACACGCACGTG-----------------TG------------------------------------TTTATAAATAAATTCGGTTAAATAGTGT---------------------TTAAGT-----------------------------------------GCGCAT--------AT-AAAT-G--------------------------TG--------------------------TGTTTGTGTGTGTG-----------------------------------------------------CTGCAGTTCCAGTGA	Size	Hit Count	Total Norm	Total	M054 Female-body	V113 Male-body	V114 Embryo	V115 Head
..............................................................................................................................................................................................................................................................................................................................................................................................AAATAAATTCGGTTAAATAGTGT---------------------..............................................................................................................................................................................................................	23	1	1.00	1	0	0	1	0
.............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................AT--------AT-AAAT-G--------------------------TG--------------------------TGTTTGTGT.........................................................................	20	1	1.00	1	0	0	1	0
..........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TG--------------------------TGTTTGTGTGTGTG-----------------------------------------------------CT.............	18	4	0.25	1	0	0	1	0
...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................G--------------------------TG--------------------------TGTTTGTGTGTGTG-----------------------------------------------------C..............	18	20	0.05	1	0	0	1	0

dm3

CTG--------GCTAATCCGA-----TCAGCT---AAGC-TCACTTAGA--ATGAAATCAAGGGCATGAGTAC-GCAGCTTAAAATAAAC-----CGAATTGACG-TA------------G---------------------G--------------------------------------AAAA---------------------GTCACAGTCGCTCT--------------------------------------------------C----------------ACACTCTAACTG---ACTAAGTACACACGCACGTG-----------------TG------------------------------------TTTATAAATAAATTCGGTTAAATAGTGT---------------------TTAATTTATAA----ATTTCCAGCTTGGACTCG--CTGTGTATGAGTGCGCAT--------AT-AAAT-GTG----TGT--TTG--TGTGT--GTTTG--------------------------TGTG--TGTGTGTG-----------------------------------------------------TTGCAGTTCCAGTGA	Size	Hit Count	Total Norm	Total	GSM154618 Ovary	GSM154620 Ovary	GSM154621 Ovary	GSM154622 Ovary	GSM180328 Head	GSM180329 Mixed-body	GSM180330 Embryo	GSM180331 Embryo	GSM180332 Embryo	GSM180333 Embryo	GSM180334 Larvae	GSM180335 Disc	GSM180336 Pupae	GSM180337 S2	GSM231091 Ovary	GSM239041 Head	GSM239050 Head	GSM239051 S2	GSM239052 S2	GSM246084 Head	GSM266765 S2	GSM280082 Ovary	GSM280083 Ovary	GSM280084 Ovary	GSM280085 Testis	GSM280086 Ovary	GSM280087 S2-NP	GSM280088 S2-NP	GSM312995 Head	GSM313160 Head	GSM313161 Head	GSM313162 Head	GSM313163 Head	GSM313164 Head	GSM313165 Head	GSM343287 Embryo	GSM379050 Ovary	GSM379051 Ovary	GSM379052 Ovary	GSM379053 Ovary	GSM379054 Ovary	GSM379055 Ovary	GSM379056 Ovary	GSM379057 Ovary	GSM379058 Ovary	GSM379059 Ovary	GSM379060 Ovary	GSM379061 Ovary	GSM379062 Ovary	GSM379063 Ovary	GSM379064 Ovary	GSM379065 Ovary	GSM379066 Ovary	GSM379067 Ovary	GSM424739 S2	GSM424740 S2	GSM424741 S2	GSM467729 Female-body	GSM467730 Female-body	GSM467731 Female-body	M002 CS	M003 Male-body	M004 Male-body	M005 ML-DmD32	M006 ML-DmD32	M029 OSC	M030 OSC	M031 OSC	M032 S2-R+	M033 S2-R+	M034 S2-R+	M035 S2-R+	M036 S2-R+	M037 S2-R+	M038 S2-R+	M039 S2-R+	M048 S2-R+	OSS2 OSS	OSS6 OSS	OSS7 OSS	OSS8 OSS	S1 Head	S10 Embryo	S11 Embryo	S12 Embryo	S13 Embryo	S14 Embryo	S15 S2-GMR	S16 Kc	S17 Larvae	S18 Larvae	S19 Pupae	S2 Head	S20 Pupae	S21 Disc	S22 Head	S23 Female-body	S24 Male-body	S25 Larvae	S26 Larvae	S27 Pupae	S28 Pupae	S29 Head	S3 Male-body	S30 S2-GMR	S31 S2	S32 S2	S33 Kc	S34 Kc	S35 Kc	S4 Female-body	S5 Disc	S6 Embryo	S7 Embryo	S8 S2	S9 Embryo	SRR001337 Head	SRR001338 Head	SRR001339 Head	SRR001340 Head	SRR001341 Head	SRR001342 Head	SRR001343 Head	SRR001344 Head	SRR001345 Head	SRR001346 Head	SRR001347 Head	SRR001348 Head	SRR001349 Head	SRR001664 Head	SRR010951 Ovary	SRR010952 Ovary	SRR010953 Ovary	SRR010954 Ovary	SRR010955 Ovary	SRR010956 Ovary	SRR010957 Ovary	SRR010958 Ovary	SRR010959 Ovary	SRR010960 Ovary	SRR014268 Embryo	SRR014269 Embryo	SRR014270 Embryo	SRR014271 Embryo	SRR014272 Embryo	SRR014273 Ovary	SRR014274 Ovary	SRR014275 Ovary	SRR014276 Ovary	SRR014277 Ovary	SRR014278 Ovary	SRR014279 Ovary	SRR014280 Ovary	SRR014281 Ovary	SRR014282 Ovary	SRR023197 S2	SRR023399 S2	SRR023400 S2	SRR023402 S2	SRR023407 S2	SRR029028 S2	SRR029029 S2	SRR029030 S2	SRR029031 S2	SRR029032 S2	SRR029033 S2	SRR029608 Head	SRR029633 Head	SRR030145 gb3	SRR031692 Head	SRR031693 Head	SRR031694 Head	SRR031696 Head	SRR031697 Head	SRR031698 Head	SRR031699 Head	SRR031700 Head	SRR031701 Head	SRR031702 Head	SRR031703 Head	SRR031704 Head	SRR032092 S2	SRR032093 S2	SRR032094 S2	SRR032095 S2-NP	SRR032096 S2-NP	SRR032152 Head	SRR060643 Testis	SRR060644 Ovary	SRR060645 Testis	SRR060646 Ovary	SRR060647 Ovary	SRR060648 Ovary	SRR060649 Ovary	SRR060650 Testis	SRR060651 Ovary	SRR060652 Testis	SRR060653 Ovary	SRR065151 Ovary	SRR065152 Ovary	SRR065153 Ovary	SRR065154 Ovary	SRR065155 Ovary	SRR065800 Ovary	SRR065801 Ovary	SRR065802 Ovary	SRR065803 Ovary	SRR065804 Ovary	SRR065805 Ovary	SRR065806 Ovary	SRR065807 Ovary	SRR097865 S2-NP	SRR097866 S2-NP	SRR097867 S2-NP	SRR298536 Ovary	SRR298537 Ovary	SRR298711 Ovary	SRR298712 Ovary	SRR317110 Ovary	SRR317111 Ovary	SRR317112 Ovary	SRR317113 Ovary	SRR317114 Ovary	SRR317115 Ovary	SRR317116 Ovary	SRR317117 Ovary	SRR341115 S2	SRR341116 S2	SRR341117 S2	SRR341118 S2	SRR351332 Mixed-body	SRR351333 Mixed-body	T004 OSC	T005 CME-L1	V001 Embryo	V002 Embryo	V003 S2	V006 Female-body	V007 Head	V008 S2	V009 CME-L1	V010 ML-DmD20-C5	V011 Sg4	V012 Male-body	V013 Head	V014 DTT	V015 DreRFHV	V018 Kc167	V019 GM2	V020 S2-R+	V021 ML-DmD21	V022 ML-DmD32	V023 Head	V024 Kc167	V025 S2-R+	V026 1182-2H	V027 ML-DmD21	V028 CME-W1-Cl.8+	V029 ML-DmD9	V030 ML-DmD8	V031 GM2	V032 S1	V033 CME-W1-Cl.8+	V034 ML-DmD16-C3	V035 ML-DmD32	V036 ML-DmD20-C5	V037 S2	V038 S2	V063 Ovary	V0632 Ovary	V064 Ovary	V0642 Ovary	V065 Ovary	V0652 Ovary	V066 Ovary	V0662 Ovary	V067 S2-NP	V073 mbn2	V074 S3	V075 ML-DmBG1-C1	V076 ML-DmBG3-C2	V077 Head	V078 Head	V079 Head	V080 Head	V081 Embryo	V082 Embryo	V083 Male-body	V084 Female-body	V085 CME-W2	V086 Female-body	V088 Ovary	V0882 Ovary	V089 Ovary	V0892 Ovary	V090 Male-body	V091 fGS/OSS	V092 Male-body	V093 Male-body	V094 S2-R+	V095 S2-R+	V096 Ovary	V097 Ovary	V098 Ovary	V125 ML-DmD9	V126 CME-L1	V127 G2	V128 S3	V129 ML-DmBG1-C1	V130 ML-DmBG3-C2	V131 ML-DmD16-C3	V132 ML-DmD32	V133 Sg4	V134 ML-DmD8	V135 CME-W2	V136 Male-body	V137 Head	V138 Male-body	V139 Female-body	V140 Female-body	V141 Female-body	V142 Female-body	V144 OSC	V145 S2	V146 S1	V147 1182-2H	V148 mbn2	GSM1528798 follicle cells
.............TAATCCGA-----TCAGCT---AAGC-TCACTTA.........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	25	1	5.00	5	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...............................................GA--ATGAAATCAAGGGCATGAGTAC-GCA...........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	27	1	2.00	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.....................................................................................................................................................................................AAAA---------------------GTCACAGTCGCTCT--------------------------------------------------C----------------ACA......................................................................................................................................................................................................................................................................................................................................................	22	1	2.00	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0
...............................................................................................................................................................................................................................................................................................................................................................................................................................................................AGCTTGGACTCG--CTGTGTATG..................................................................................................................................................................	21	1	2.00	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0
...................................................................................................TTGACG-TA------------G---------------------G--------------------------------------AAAA---------------------GTCACAGTCGC...............................................................................................................................................................................................................................................................................................................................................................................................................................	25	1	2.00	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..................................................................................AAATAAAC-----CGAATTGACG-TA------------G---------------------..........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	21	1	2.00	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0
............................................................................................................................................................................................................................................................................................................................................................................................................................................................TCCAGCTTGGACTCG--CTGTGTATGAGT...............................................................................................................................................................	27	1	2.00	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0
........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TGCGCAT--------AT-AAAT-GTG----TGT--TTG--TG......................................................................................................................	24	1	2.00	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
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..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TGT--GTTTG--------------------------TGTG--TGTGTGTG-----------------------------------------------------TT.............	22	6	0.17	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0
...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GT--GTTTG--------------------------TGTG--TGTGTGTG-----------------------------------------------------TT.............	21	7	0.14	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0
...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GTG----TGT--TTG--TGTGT--GTTTG--------------------------T.................................................................................	20	9	0.11	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TG----TGT--TTG--TGTGT--GTTTG--------------------------TG................................................................................	20	9	0.11	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0
................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TG----TGT--TTG--TGTGT--GTTTG--------------------------T.................................................................................	19	10	0.10	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GT--TTG--TGTGT--GTTTG--------------------------TGTG--............................................................................	19	10	0.10	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0
.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GTGT--GTTTG--------------------------TGTG--TGTGTGTG-----------------------------------------------------...............	21	20	0.10	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TGT--GTTTG--------------------------TGTG--TGTGTGTG-----------------------------------------------------T..............	21	20	0.10	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0
...........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TTG--TGTGT--GTTTG--------------------------TGTG--TG..........................................................................	19	13	0.08	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0

droEre2

CTG--------GCTAATCCGA-----TCAGCT---AAGC-TCACTTAGA--ATGAAATCAAGGGCATGAGTAC-GCAGCTTAAAATAAAC-----CGAATTGACG-TA------------G---------------------A--------------------------------------AAAA---------------------GTCACAGTCGCTCT--------------------------------------------------C----------------ACACTCTAACTA---ACTAAGTACACACGCACGTG-----------------TG------------------------------------CTTATAAATAAATTCGGTTAAATAGTGT---------------------TTAATTTATAA----ATTTCCAGCTTGGACTCTCGTTGTGTGTTAGTGAGCAT--------AT-A---TGTA----TATATCTA--TGTGT--GGTTG--------------------------TGTG--CGTGTGTG-----------------------------------------------------TTGCAGTTCCAGTGA	Size	Hit Count	Total Norm	Total	M055 Female-body	M057 Embryo	V060 Head	V040 Embryo	V107 Male-body	V108 Head	GSM1528801 follicle cells
......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................AGTGAGCAT--------AT-A---TGTA----TATAT.............................................................................................................................	21	1	1.00	1	0	0	0	0	1	0	0
...............................................................................................................................................................................................................................................................................................................CTAAGTACACACGCACGTG-----------------T....................................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	0	0	1	0	0	0
..........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TG--------------------------TGTG--CGTGTGTG-----------------------------------------------------TTGC...........	18	3	0.33	1	0	0	0	0	0	1	0
...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GT--GGTTG--------------------------TGTG--CGTGTGT.....................................................................	18	5	0.20	1	0	0	0	0	0	1	0

droYak3

CTG--------ACTAATCCGA-----TCAGCT---AAGC-TCACTTAGA--ATGAAATCAAGGGCATGAGTAC-GCAGCTTAAAATAAAC-----CAAATTGACG-TA------------G---------------------G--------------------------------------AAAA---------------------GTCACAGTCGCTCT--------------------------------------------------C--------------ACACTCTCCAACTG---ACTAAGTACACACGCACGTG-----------------TG------------------------------------TTTATAAATAAATTCGGTTAAATAGTGT---------------------TTAATTTATAA----ATTTCCAGCTTGGACTCTCGCTGTATGTTAGTGCCCAT--------CT-ACAT-ATA------T--CTGTGTGTA------TG--------------------------TGTG--CGTGTGTG-----------------------------------------------------TTGCAGTTCCAGTGA	Size	Hit Count	Total Norm	Total	M026 Head	M043 Female-body	M056 Embryo	V046 Embryo	V052 Head	V058 Head	V120 Male-body	SRR1275488 Male larvae	SRR1275486 Male prepupae	SRR1275484 Male prepupae	GSM1528802 follicle cells
...................GA-----TCAGCT---AAGC-TCACTTAGA--AT...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	23	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0
...............................................................................................................................................................................................................TCACAGTCGCTCT--------------------------------------------------C--------------ACACTCTCCAA................................................................................................................................................................................................................................................................................................................................................	25	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
........................................................ATCAAGGGCATGAGTAC-GC............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
................................................................................................................................................................................................................................................................................................................................................................................................................................................................GCTTGGACTCTCGCTGTATGTTA.................................................................................................................................................................	23	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................A------TG--------------------------TGTG--CGTGTGTG-----------------------------------------------------TTGCAGTTCCAGT..	28	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
..................................................................................................................................................................................................................................................................................................................................................................................................AAATTCGGTTAAATAGTGT---------------------..............................................................................................................................................................................................................	19	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
.....................................................GAAATCAAGGGCATGAGTAC-GC............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
.....................................................GAAATCAAGGGCATGAGTAC-..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
............CTAATCCGA-----TCAGCT---AAGC-T...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ATA------T--CTGTGTGTA------TG--------------------------TGTG--CGTGT.......................................................................	24	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0

droEug1

C------------TAATCTGA-----TCAGCG---G--AG-AGCTTAGA--ATGAAATCAAGGGCATGAGTAC-GCAGCTTAAAATAATT-----CAAATTGACG-TA------------G---------------------G--------------------------------------AAAA---------------------GTCACAGTCACAGT------T--ACA------AACGT-------A--------------T----C----------------ACTCTCTAAATA---ACTAAGTACACACGCACGTG-----------------TG------------------------------------TTTATAAATAAATTTGGTTAACTAGTGT---------------------TTAATTTATAA----ATTTCCATTCTGAT---------------------------------------TGTA----TGT---GGTGTGTGT--GTCTG--------------------------TGTT--TGTGTGTG-----------------------------------------------------TTGCAGTTCCAGTGA	Size	Hit Count	Total Norm	Total
No Reads

droBia1

CTG--------GCTAATCTGA-----TCAGCAGCG---CG-AGCTTAGA--ATGA-ATCAAGGGCATGAGTAC-GCAGCTTAAAATAAGC-----CAAATTGACG-TA------------G---------------------G--------------------------------------AAAA---------------------GTCACAGTCGCAGT------C--ACAGTTCCACTCGCA----------------------------------------GGCCCTCTCTAAGTA---GCTGGGTACACAGGCACGTG-----------------TG------------------------------------TTTGTAAATAAATTCGGTTAAATAGTGT---------------------TTAATTTGCGA----ATCTCCAGCCA-------------------------------------------------------C--------------TG--------------------------TGCC--TGTGTGTG-----------------------------------------------------TTGCAGTTCCAGTGA	Size	Hit Count	Total Norm	Total
No Reads

droTak1

CTG--------GCTAATCTGA-----TCAG---CG---AG-AGCTTAGA--ATGAAATCAAGGGCATGAGTAC-GCAGCTTAAAATAAGC-----CAAATTGACG-TA------------G---------------------G--------------------------------------AAAA---------------------GTCACAGTTACGGTCACAGTT--ACA------GTCGCAGCTCGCA----------------------------------GCACTCTCTAAATA---ACTAAGTACACACGCACGTG-----------------TG------------------------------------TTTATAAATAAATTCGGTTAAATAGTGT---------------------TTAATTTATAA----ATTTCCAGCTTG-------------------------------------------TG------T--CTGTGTCTGT--GCCTG--------------------------TGTT--TGTGTGTG-----------------------------------------------------TTGCAGTTCCAGTGA	Size	Hit Count	Total Norm	Total
No Reads

droEle1

CTG--------GCTAATCTGA-----TCAA---CG---GG-AGCTTAGA--ATGAAATCAAGGGCATGAGTAC-GCAGCTTAAAATAAAC-----CGAATTGACG-TA------------G------------------------------------------------------------AAAA---------------------GGCACAGTCACAAT------C--ACA------GTCG----------------------------C----------------ACTCTCTAAAT----ACTAAGTACACACGCACGTG-----------------TG------------------------------------TTTATAAATAAATTCGGTTAAATAGTGT---------------------TTAATTTATAA----ATTTCCAGCCTGGTTTGG------------------------------------------------CTGTGCATA----------------------------------------TGTGTGTG-----------------------------------------------------TTGCAGTTCCAGTGA	Size	Hit Count	Total Norm	Total
No Reads

droRho1

CTA--------GCTAATCTGA-----TCAA---CG---AG-AGCTTAGA--ATGAAATCAAGGGCATGAGTAC-GCAGCTTAAAATAAAC-----CAAATTGACG-TA------------G---------------------A--------------------------------------AAAA---------------------GTCACAGTCACAAT------C--ACA------GTCG----------------------------C----------------ACTCTCTAAAT----ACTTAGTACACACGCACGTG-----------------TG------------------------------------TTTATAAATAAATTCGGTTAAATAGTGT---------------------TTAATTTAAAA----ATTTCCAGCCTGGTTTGG------------------------------------------------CTGTGTATA----------------------------------------TGTGTGTG-----------------------------------------------------TTGCAGTTCCAGTGA	Size	Hit Count	Total Norm	Total
No Reads

droFic1

CTG--------GCTAATCTGA-----TCAA--GCG---AG-AGCTTAGA--ATGAAATCAAGGGCATGAGTAC-GCAGCTTAAAATAAAC-----CAAATTGACG-TA------------G---------------------G--------------------------------------AAAA---------------------GTCACAGTCACAGT------TAAACA------GTCG----------------------------C----------------ACTCTCTAAGT----ACTAAGTACACACGCACGTG-----------------TG------------------------------------TTTATAAATAAATTCGGTTAACTAGTGT---------------------TTAATTTATAA----ATTTCCAGCCTGGGCTGGCA------------------------------------G------T--CTGTGTATG----TGTG--------------------------T--G--TTTGTGTA-----------------------------------------------------TTGCAATTCCAGTGA	Size	Hit Count	Total Norm	Total
No Reads

droKik1

CTG--------GTTAATCTGATCTGATCAG---CGA--GC-AGCTCAGA--ATGAAATCAAGGGCATGAGTAC-GCAGCTTAAAATAAAC-----CAAATTGACG-CG------------G---------------------G--------------------------------------GAAAGGTCACGGGAACCGCGGAGCAGTCACAGTCACAAT------TT-AC--------------------------------------------------------AGTCACTAAATATGTACAAAGTACACACGCACGTG-----------------TGCGGCGGGT-------------------T---CTAT-TTTCGAAGTAAATTCGGTTAAATAGTGT---------------------TTAATTTATAA---AATTTCCAGAGTGAGCTT------------------------GGCTTATGAAAT-GTG--TGT------GTATTTGT--GTATGGGT------TTACGTACTC--A-ATATGCG--TGTGTGTG-----------------------------------------------------TTGCTGTTCCAGTGA	Size	Hit Count	Total Norm	Total
No Reads

droAna3

CTGCCTTCCGAGCTAATCTGA-----TCAGC--------G-AGCTTAGA--ATGAAATCAAGGGCATGAGTAC-GCAGCTTAGAATAAGCAGGCCCCAATTGAG--T------GTGCCACAAAG------------------GGGGCGGGGAAGGGGTG-------------GTGGTGTCCAGGG---------------------GTCACGGGA-ATTT--------------------------------------------------C----------------AGTCTCTAAAC----GCAAAACACACACGCACGTG-----------------TCAGCTGGGTCTCGGGATCGGGTCTCTGGTCTGCTCGATTTAAAAATAAATTCGATTAAATAGTGTTTCTTTTCCTTTATTTATTTTTTAATTTATGGATGAATTTCAAAG------------------------CCCAGCTGAGCT--------TGTGTGTGT------GTGTGTGT--GTGTGTGTTTGTTGTTCCGCGTATAAGTGTATCTGTGTGAGTGTG-----------------------------------------------------TTGCAGTTCCAG--A	Size	Hit Count	Total Norm	Total	M044 Female-body	M058 Embryo	V039 Embryo	V055 Head	V105 Male-body	V106 Head
..............................................................................................................................................GGGGCGGGGAAGGGGTG-------------GTGG........................................................................................................................................................................................................................................................................................................................................................................................................................................................................	21	1	2.00	2	0	0	0	0	0	2
..............................................................................................................................................GGGGCGGGGAAGGGGTG-------------GTGGT.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................	22	1	2.00	2	0	0	0	0	0	2
.....................................................................................................................................................................................................................................................................................................................................................AGCTGGGTCTCGGGATCGGGTT.............................................................................................................................................................................................................................................................................	22	1	1.00	1	1	0	0	0	0	0
...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GT--GTGTGTGTTTGTTGTTCCGC.............................................................................................	22	1	1.00	1	1	0	0	0	0	0
..............................................................................................................................................GGGGCGGGGAAGGGGTG-------------GTGGTG......................................................................................................................................................................................................................................................................................................................................................................................................................................................................	23	1	1.00	1	0	0	0	0	0	1
.........................................................................................................................AAG------------------GGGGCGGGGAAGGGGTG-------------GTGG........................................................................................................................................................................................................................................................................................................................................................................................................................................................................	24	1	1.00	1	0	0	0	0	0	1
..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GCTGAGCT--------TGTGTGTGT------G..........................................................................................................................	18	1	1.00	1	0	0	0	0	1	0
..............................................................................................................................................GGGGCGGGGAAGGGGTG-------------GTGC........................................................................................................................................................................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	1
.........................................................TCAAGGGCATGAGTAC-GCAGC.........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	1	0	0
........................................................................................................................................................................................................................................................................................................................CACGCACGTG-----------------TCAGCTGGGTCC.........................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	0	0	0	1
................................................................................................................................................................................................................................................................................................................................................................GGGATCGGGTCTCTGGTC......................................................................................................................................................................................................................................................................	18	1	1.00	1	1	0	0	0	0	0
..............................................................................................................................................GGGGCGGGGAAGGGGTG-------------GTGGA.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	0	0	0	1
...........................................................................................................................G------------------GGGGCGGGGAAGGGGTG-------------GTGG........................................................................................................................................................................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	0	0	0	1
...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GCT--------TGTGTGTGT------GTGTGTGT--GTGTGT...........................................................................................................	26	4	0.25	1	0	0	0	0	0	1
.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GTGTGT------GTGTGTGT--GTGTGTGTTTGT.....................................................................................................	26	20	0.05	1	0	0	0	0	0	1

droBip1

CAA--------GCTAATCTGA-----TCAG---TG---CG-AGCTTAGA--ATGAAATCAAGGGCATGAGTAC-GCAGCTTAGAATAAGC----CCCAATTGAGC-TC------------CAAGTGTGTGGTGGTGGCTCGGAGGGCGGTGGTGGGGTGTGCAACTGTGTGTGTGGCGGCCAGGG---------------------GTCACGGG-------------------------------------------------------------------------AATTTCTAAAC----GCAAAATACACACGCACGTGTGTGCCGTCG---------------TT-------------------T---CTCG-ATTAAAAATAAATTCATTTAAATAGTGTTGCTTT-----------TTTTTTAATTTATGG----ATAA--A-------------------------GCCCAG--------CT-GAGT-GTG----T------------GT--GTGTGTGTTTGTTGTTCCGTGTTT--GTGTATGTG--TGTGTGTG-----------------------------------------------------TTGCAGTTCCAGT--	Size	Hit Count	Total Norm	Total
No Reads

dp5

ATA--------GGTATTACGACTGTGCCGG--ACA---GG-GGCTTGAA--ATGACATCAAGGGCAACAGGACAGCGGCCAGGAGAGGGT-----GGGATTGAGGGTG------------G---------------------G-------------------------------------------------------------------CCGTCACAAA------T--------------------------------------------------------------------------------ACACACACGCACGTG-----------------CGG--------------------------A---CTTG-TTTAAAAATAAATTCGGTTAAATAGTGT---------------------TTAATTTATAA----ATTTTAAA---------------------------------------------TGTA----TGC--CAGTGTGTGT--GTGTG--------------------------CGTG--CGTGTGTGCGAGAGAGAAAGAGATTAGAGCAGAGCAAAAAAACCAACAGGGCAATAACAAGCAACAATTCCAGAGG	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
........................................................................................GT-----GGGATTGAGGGTG------------G---------------------G-------------------------------------------------------------------CC....................................................................................................................................................................................................................................................................................................................................................................................................................................	19	1	4.00	4	0	2	0	0	0	0	1	1	0	0
........................................................................................GT-----GGGATTGAGGGTG------------G---------------------G-------------------------------------------------------------------C.....................................................................................................................................................................................................................................................................................................................................................................................................................................	18	1	4.00	4	0	2	0	1	0	0	0	0	1	0
....................................................................................GAGGGT-----GGGATTGAGGGTG------------G---------------------G-------------------------------------------------------------------......................................................................................................................................................................................................................................................................................................................................................................................................................................	21	1	2.00	2	0	1	0	0	0	0	1	0	0	0
..........................................................................GCGGCCAGGAGAGGGT-----GGG......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	19	1	2.00	2	0	0	0	0	0	1	1	0	0	0
...............................................................................CAGGAGAGGGT-----GGGATTGAGGGTG------------................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	24	1	1.00	1	0	1	0	0	0	0	0	0	0	0
....................................................................................GAGGGT-----GGGATTGAGGGT.............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	18	1	1.00	1	0	1	0	0	0	0	0	0	0	0
......................................................ACATCAAGGGCAACAGGACAG.............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	1	0	0	0	0	0	0	0	0
...........................................................AAGGGCAACAGGACAGCGG..........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	0	0	0	0	0	0	1	0	0	0
....................................................................................GAGGGT-----GGGATTGAGGGTG------------................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	0	1	0	0	0	0	0	0	0	0
....................................................................................GAGGGT-----GGGATTGAGGGTG------------G---------------------G-------------------------------------------------------------------C.....................................................................................................................................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	1	0	0	0	0	0	0	0
...............................................................................................GGGATTGAGGGTG------------G---------------------G-------------------------------------------------------------------CCGTCAC...............................................................................................................................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	0	0	0	0	1	0	0	0
................................................................................AGGAGAGGGT-----GGGATTGAGGGTG------------................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	23	1	1.00	1	0	1	0	0	0	0	0	0	0	0
..........................................................................GCGGCCAGGAGAGGGT-----GGGA.....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	0	0	0	0	0	1	0	0	0
.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GTGTG--------------------------CGTG--CGTGTGTGCG..................................................................	19	5	0.20	1	0	1	0	0	0	0	0	0	0	0

droPer2

ATA--------GGTATTACGACTGTGCCGG--ACA---GG-GGCTTGAA--ATGACATCAAGGGCAACAGGACAGCGGCCAGGAGAGGGT-----GGGATTGAGGGTG------------G---------------------G-------------------------------------------------------------------CCGTCACAAA------T--------------------------------------------------------------------------------ACACACACGCACGTG-----------------CGG--------------------------A---CTTG-TTTAAAAATAAATTCGGTTAAATAGTGT---------------------TTAATTTATAA----ATTTTAAA---------------------------------------------TGTA----TGC--CAGTGTGTGT--GTGTG--------------------------CGTT--CGTGTGTGCGAGAGAGAAAGAGATTAGAGCAGAGCAAAAAAACCAACAGGGCAATAACAAGCAACAATTCCAGAGG	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TGTGTGT--GTGTG--------------------------CGTT--CGTGT.......................................................................	21	2	3.00	6	0	1	0	0	0	5
....................................................................................GAGGGT-----GGGATTGAGGGTG------------G---------------------..........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	20	1	2.00	2	0	0	0	0	0	2
.......................................................CATCAAGGGCAACAGGACAG.............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	1	0	0	0	0
....................................................................................GAGGGT-----GGGATTGAGGGT.............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	18	1	1.00	1	0	0	0	0	0	1
........................................................................................GT-----GGGATTGAGGGTG------------G---------------------G-------------------------------------------------------------------CCGTCACAA.............................................................................................................................................................................................................................................................................................................................................................................................................................	26	1	1.00	1	0	0	0	0	0	1
..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TGTGTGT--GTGTG--------------------------CGTT--CGTG........................................................................	20	2	1.00	2	0	0	0	0	0	2
...................................................................................AGAGGGT-----GGGATTGAGGGT.............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	0	0	0	0	0	1
..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TGTGTGT--GTGTG--------------------------CGTT--CGT.........................................................................	19	2	0.50	1	0	0	0	0	0	1
...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GTGTGT--GTGTG--------------------------CGTT--CGTG........................................................................	19	4	0.25	1	0	0	0	0	0	1
..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TGTGTGT--GTGTG--------------------------CGTT--CG..........................................................................	18	5	0.20	1	0	1	0	0	0	0

droWil2

ATC--------TCTTTTTCGCATA--TTAG--ACA---AATAATTTAAACCTTAAAATCAAGTGCATGCGTACGCTTATATAGAGGGAGT-----GGAAAAGTGTGTGTGAGAGAGAGACACAG------------------AGAGTGAGCAACGG--------------------------------------------------------------------------------------------GCAGGGCTGACACACACACACATACACACAAACACAAAACCTG-----------------------------TGTATAACATTGATTCGTCTA------------------------------------TTTAAAAATAAATTCAGTTAAATAGTGT---------------------TTAATTTATAA----ATTTTGTA---------------------------------------------TGTG----TCT--ATG--TATGTGTGTGTG--------------------------TGTG--CCTGAGTG------------------------------------------------------------------GG	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
...............................................................................................................................................................................................................................................................................................................................................................................................AATAAATTCAGTTAAATAGTGT---------------------TTA...........................................................................................................................................................................................................	25	1	1.00	1	0	0	0	1	0
........................................................................................GT-----GGAAAAGTGTGTGTGA.........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	18	1	1.00	1	0	0	1	0	0
.......................................................................................................................................................AACGG--------------------------------------------------------------------------------------------GCAGGGCTGACAC...................................................................................................................................................................................................................................................................................................................................................................................	18	1	1.00	1	0	0	0	1	0
............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TG--TATGTGTGTGTG--------------------------TGTG--............................................................................	18	20	0.20	4	0	0	0	0	4

-4.2	-3.9	-3.9	-3.5

dsi_14207

2r:16001661-16001810 +

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads