dsi_13210

CTCGCCGCACACGGGAGCCGGAGGATCTAGCTCGTCTAGCTCTGGATCGGGTGGACCGTCCTCCTCGGACCGACATCATGGCCCACCACCGCCGACCATGTCGATGAAGCACATTGTACGCTCTGGTGGCATGTATCGCGGTGACACCGTAACCGTTCCTTCGCTGGCTGCACCCAGTTCGTATCTGTATCCCACACCGCCGCACGGAGTGGTCAAGGCCATCGGCGGCGGTGGCGTGGTGCCCGGTGTG **********************************************************************************************(((((((....(((.........)))....)))))))............*******************************************************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M053 female body	SRR618934 dsim w501 ovaries	SRR553486 Makindu_3 day-old ovaries	M023 head	M025 embryo	M024 male body	GSM343915 embryo	SRR553488 RT_0-2 hours eggs	SRR553485 Chicharo_3 day-old ovaries	SRR553487 NRT_0-2 hours eggs	SRR902009 testis
............................................................................................................................................................................................................CGGAGTTGTCAAGGACATC...........................	19	2	2	2.50	5	0	0	0	4	1	0	0	0	0	0	0
...........................................................................................................................................................................................................ACGGAGTGGTCAAGGCCATCG..........................	21	0	1	2.00	2	0	2	0	0	0	0	0	0	0	0	0
................................................................................................CATGTCGATGAAGCACATTGTACG..................................................................................................................................	24	0	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0
.....................................................................................................................................TATCGCGGTGACACCGTAACTGT..............................................................................................	23	1	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
...............................................................................................................................GGCATGTATCGCGGTGAC.........................................................................................................	18	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0
...................................................................................................................................................................................................................GTCAAGGCCATCGGCGGCGGT..................	21	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0
............................AGCTCGTCTAGCTCTGGAT...........................................................................................................................................................................................................	19	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0
..............................................................................................................................TGGCATGTATCGCGGTGACACCGTA...................................................................................................	25	0	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0
.................................................................................................................................................ACCGTAACCGTTCCTTCGCTG....................................................................................	21	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0
.......CACACGGGAGCCGGAGGATCTAGCT..........................................................................................................................................................................................................................	25	0	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0
.........................................................................................................................................................................................................GCACGGAGTGGTCAAGGCCATCGGCGGC.....................	28	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0
............................AGCTCGTCTAGCTCTGGATCGGGTGGACC.................................................................................................................................................................................................	29	0	1	1.00	1	0	0	0	0	0	1	0	0	0	0	0
..CGCCGCACACGGGAGCCGGAG...................................................................................................................................................................................................................................	21	0	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
......................................................................................................................................................................................................................................GTCGCGTGGTGTCCGGTG..	18	2	2	0.50	1	0	0	1	0	0	0	0	0	0	0	0
...........................................................................................................................................................................................................GCGGAGTTGTCAAGGACATC...........................	20	3	10	0.50	5	0	0	0	0	5	0	0	0	0	0	0
..........................................................TTCTCCTCGGACCGATATCG............................................................................................................................................................................	20	3	2	0.50	1	0	1	0	0	0	0	0	0	0	0	0
..........................................................................................................................................................................................................CGCGGAGTTGTCAAGGACATC...........................	21	3	4	0.50	2	0	0	0	0	2	0	0	0	0	0	0
.......................................CTATGGATCGGGTGG....................................................................................................................................................................................................	15	1	5	0.40	2	0	0	0	0	0	0	0	1	1	0	0
.......CACGCGCGAGCCGGAGGA.................................................................................................................................................................................................................................	18	2	6	0.17	1	0	0	1	0	0	0	0	0	0	0	0
.....................................................................................................................................................................................................................CAAGGCCCTCGGCGG......................	15	1	7	0.14	1	0	0	0	0	0	0	0	1	0	0	0
............................................................................CATGGGCCATCACCGCTGA...........................................................................................................................................................	19	3	12	0.08	1	0	1	0	0	0	0	0	0	0	0	0
.............................................................................................................................................................................................................................TCGGCGGCGTTGGCGTGTTT.........	20	3	13	0.08	1	0	0	0	0	0	0	0	0	0	0	1
......................................................................................CACCGCCGGCCAAGTCGA..................................................................................................................................................	18	2	14	0.07	1	0	0	0	0	0	0	0	1	0	0	0
.....................................................................................................................................................................................................................................CGTCGCGTGGTGTCCGGTG..	19	3	15	0.07	1	0	1	0	0	0	0	0	0	0	0	0
............................................................................................................................................................................................................CGGAGTTGTCAAGGACA.............................	17	2	16	0.06	1	0	0	0	0	0	0	0	0	0	1	0
...........................................................................................................................................................................................................ACGGAATAGTCAAGGC...............................	16	2	20	0.05	1	0	0	0	0	0	0	0	0	0	1	0
.....................................................................................................................................................................................................................................GGTAGCGTGGCGCCCG.....	16	2	20	0.05	1	0	1	0	0	0	0	0	0	0	0	0
....................GGGGATGTAGCTCGTC......................................................................................................................................................................................................................	16	2	20	0.05	1	0	1	0	0	0	0	0	0	0	0	0
.............................GCTGGTCTAGCTCAGG.............................................................................................................................................................................................................	16	2	20	0.05	1	0	0	0	0	0	0	0	1	0	0	0
.............................................GTCGGGTCGAACGTCCTC...........................................................................................................................................................................................	18	3	20	0.05	1	0	0	0	0	0	1	0	0	0	0	0

GAGCGGCGTGTGCCCTCGGCCTCCTAGATCGAGCAGATCGAGACCTAGCCCACCTGGCAGGAGGAGCCTGGCTGTAGTACCGGGTGGTGGCGGCTGGTACAGCTACTTCGTGTAACATGCGAGACCACCGTACATAGCGCCACTGTGGCATTGGCAAGGAAGCGACCGACGTGGGTCAAGCATAGACATAGGGTGTGGCGGCGTGCCTCACCAGTTCCGGTAGCCGCCGCCACCGCACCACGGGCCACAC *******************************************************************************************************(((((((....(((.........)))....)))))))............**********************************************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	SRR618934 dsim w501 ovaries	SRR553485 Chicharo_3 day-old ovaries	SRR553486 Makindu_3 day-old ovaries	SRR553487 NRT_0-2 hours eggs	SRR553488 RT_0-2 hours eggs
....................................................................TGGCTGTAGTACCGGGTGGTG.................................................................................................................................................................	21	0	1	1.00	1	0	1	0	0	0
............................................................................................................................................................................GGGTCAAGCATAGACATAGGGT........................................................	22	0	1	1.00	1	1	0	0	0	0
...........................................................................................................TCGTGTAGCAGGCGACACCAC..........................................................................................................................	21	3	5	0.20	1	0	0	1	0	0
..................................................................................................................................................................................................GCGGCGGCGTTCCTTACCA.....................................	19	3	15	0.20	3	3	0	0	0	0
...................................................................................................CAGCTACTACGTGGAAC......................................................................................................................................	17	2	9	0.11	1	0	0	0	1	0
................................................................................................................................................................AGCGACCGACGTGCGT..........................................................................	16	1	11	0.09	1	0	1	0	0	0
...........................................................................AGTGCCGGGCGGTGGCG..............................................................................................................................................................	17	2	11	0.09	1	0	0	0	0	1
..............................................................GGCGCATGGCAGTAGTAC..........................................................................................................................................................................	18	3	20	0.05	1	1	0	0	0	0

Species	Coordinate	ID	Alignment
droSim2	x:11941120-11941369 -	dsi_13210	CTCGCCGCACACGGGAG---------CCGGAGGATC------------------TAGCTCGTCTAGCTCTGG------------------ATCGGGTGGACCG---TCCT------CCTCGGACCGACATCATGGCCCACCACCGCCGACCATGT------CGATGAAGCACATTGTACGCTCTGGTGGCATGTATCGCGGTGACACCGTAACCGTTCCTTCGCTGGC---------------------TGCA---CCCAGTTC---GTATCTGTATCCCA---CACCGCCGCACGGAGTGGTCAAGGCCATCGGCGGCGGTGGCGTGGTGCCCGGTG------TG
droSec2	scaffold_21:339714-339963 -		CTCGCCGCACACGGGAG---------CCGGAGGATC------------------TAGCTCGTCTAGCTCTGG------------------ATCGGGTGGACCG---TCCT------CCTCGGACCGACATCATGGCCCACCACCGCCGACCATAT------CGATGAAGCACATTGTACGCTCTGGTGGCATGTATCGCGGTGACACCGTAACCGTTCCTTCGCTGGC---------------------TGCA---CCAAGTTC---GTATCTGTATCCCA---CACCGCCGCACGGAGTGGTCAAGGCCATCGGCGGCGGTGGCGTGGTGCCCGGTG------TG
dm3	chrX:12585336-12585576 -		TTCGCCGCACACGGGAG---------CCGGAGGATC------------------TAGCTCGTCTGGCTCTGG------------------ATCGGGTGGACCG---TCCT------CATCGGATCGACATCATGGCCCACCACCGCCGACCATGT------CGATGAAGCACATTGTACGCTCTGGTGGCATGTATCGCGGTGACACCGTAACTGTTCCATCGCTGGC---------------------TGCA---CCCAGTTC---GTATCTGTATCCCA-----------CACGGAGTG-TCAAGACCATTGGCGGCGGTGGCGTGGTGCCCGGTG------TG
droEre2	scaffold_4690:14274107-14274353 +		CTCGCCGCACACGGGCG---------CCGGCGGATC------------------TG---------GCTCCGT------------------GTCGGGCGGACCG---TCCT------CCGCGGACCGACACCATGGCCCGCCGCCGCCGACCATGC------CGATGAAGCACATCGTCCGCTCTGGCGGCATGTATCGCGGTGACACCGTGAGCGTGCCCTCGCTGGC---------------------TGCG---CCCAGCTC---CTATCTGTATCCCA---CACCGCCGCACGGCGTGCTCAAGGCCACCGGCGGCGTTGGCGGCGTCGGCGGCGTCGGCGTC
droYak3	X:6862183-6862435 -		CTCACCGCACACGGGTG---------CCGGTGGCTC------------------GGGCT---CCGGCTCCGG------------------CTCGATTGGAGCA---TCGTCCTCCGCCGCAGAACGACATCATGGCCCCCCGCCGCCGACCATGT------CGATGAAGCACATTGTACGCTCGGGCGGCATGTATCGCGGCGACACCGTCACCGTTCCATCGCTGGC---------------------CGCG---CCCAGTTC---GTATCTGTATCCCA---CACCGCCGCACGGCGTGCTCAAGGCCACCGGCGGCGTTGGCGTGGTGCCCGGCG------TG
droEug1	scf7180000409110:974977-975203 -		TTCCCCGCATACGGGTG---------CCGGTGGTTT------------------GAGC------AGTTCTGG------------------ATCGGGTGGTCCACCCACAT------CATCGGATCGACATCATGGTCCACCACCACCGACCATGT------CAATGAAGCACATTGTCCGCTCGACGGGCATGTATCGTGGCGATGCCGTAACCGTTCCTTCACTCGC---------------------AGCG---CCCAGTTC---GTATCTGTATCCGA---CACCACCGCATGGAGTGCTTAAGGCCACCGGTGGTG--------------------------
droBia1	scf7180000298389:164285-164534 -		CTCACCGCACACGGGGG---------CAGGAGGCTCGGC---------------GGGC------GGCTCCGG------------------AGCCGGAGGATCCTCCTCTT------CGGCGGATCGCCATCACGGGCCACCGCCGCCGACCATGT------CGATGAAGCATATTGTCCGCTCCACGGGCCTGTATCGCGGCGATACCGTCAATGTACCCTCGTTGGC---------------------GGCG---CCCAATTC---GTATCTGTATCCCA---CACCGCCGCACGGCGTGCTGAAGGCCCCCGGCGGCGTGGGCGTGGTGCCCGGCG------TC
droTak1	scf7180000413903:109089-109347 +		CTCGCCGCACACGGGTG---GAT---------CATCGGCAGCAGCAGGGGGATCCAGC------AGCTCCTC------------------CTCCACTTCATCC---TCTT------CAGCGGATCGCCATCACGGACCGCCGCCGCCAACCATGT------CAATGAAGCATATTGTACGCTCAGCCAACATGTATCGCGGCGAAACAGTGAGTGTTCCCTCGCTGGC---------------------GGCG---CCCAGTTC---GTATCTGTATCCCA---CTCCACCGCATGGAGTTCTCAAGGCCACCGGTGGCGTGGGTGTGGTGCCCGGTG------TG
droEle1	scf7180000491023:271209-271473 -		TTCACCGCACACCGG---------------TGGTTC------------------GAGTGGATCCGGATCGGGATCGGGATCGGGTGCGGG---TGCGGGCGTT---TCAT------CCACCGATCGCCATCATGGCCCACCACCGCCCAGCATGT------CGATGAAGCATATTGTACGCTCCACGAACATGTATCGCGGCGACACCGTTGGCGTCCCATCGCTGGC---------------------GGCACCACCCAACTC---CTATCTGTATCCCA---ATCCGCCGCATGGCGTGCTCAAGGCCGCCGGCGGTGTGGGCGTCGTGCCGGGCGT---TGTG
droRho1	scf7180000779985:87014-87263 +		TTCGCCGCACACCGGCG---GAG---CCGGCGGATC------------------GGGTGGATCGGG---GGG------------------ATCTGTTGGACCC---TCGT------CCTCGGATCGTCACCATATCCCACAGCCACCGAGCATGT------CGATGAAGCACATGGTTCGCTCCACGAGCATGTATCGTGGCGATACCGTGACCGTGCCTTCGCTGGC---------------------AGTG---CCCAACTC---GTATCTGTATCCCA---CGACGACGCACGGCGTCCTCAAGACGACCGGTGGCGTGGGCGTGGTGCCCGGCG------TG
droFic1	scf7180000454050:489417-489687 -		CTCACCGCACACGGGCG---GCTCCTCCGGCGGATC------------------GAGTGGATCCGGCTCGGTCACCGGATCGAGTG---GCTCGGGCGGTGCC---TCCT------CGTCGGATCGCCATCATGGTCCGCCGCCGCCCGGCATGT------CGATGAAGCACATCGTCCGCTCCACGACCATGTATCGTGGCGATGCCGTGAGTGTGCCCACGCTGGC---------------------GGGT---CCCAGTTC---GTATCTGTACTCCA---CTCCGCCGCACGGCGTGCTCAAGGCCACCGGCGGAGTGGGCGTGGTGCCCGGCG------TC
droKik1	scf7180000302621:127049-127252 +		CTCACCGCACACGGCCG---------GAGGAGGA---------GGAGGA---GGCAGTTCCT---------------------------------------CA---ACCT------CCACAGAACGTCATCATGGACCGCCGCCACCCAGCATGT------CCATGAAGCACATAGTGCGCTCCACGGCCATGTATCGAGGGGATACCGTAACAGTGCCCTCGCTGAC---------------------AGCT---CCTAGCTC---GTATCTATACGCCC---AGCCGCCTCATGGCGTGCTCAAAGG-------------------------------------
droAna3	scaffold_13117:3503452-3503692 -		TTCCCCGCACACGGGCG---GTC---CGGGCGGAGG------------------AG---------GCTCATC------------------GTCGAGTGTCTCG---TCGG---TGTCGTCGGACCGCCACCATGTGACGCC---ACCTATCATGTCCAGTGCCGTGAAGCACATTGTCCGCTCTACGGGCATGTATCGCGGGGAGG------------------TGCC---------------------GCCC---CCCAACTC---GTATCTGTATCCCCAGCCGCCGCCCCACGGCGTCCTGAAGGCCACCGGCGGAGTGGGCGTCGTTCCCGGAGTGGGTGTG
droBip1	scf7180000395895:51773-52004 +		TTCGCCGCACACGGGTC---------CGGGTGGTGG------------------CG---------GCTCCTC------------------GTCGATTGCCTCG---TCTG---TCTCCTCGGACCGCCACCATGTGGCGCC---ACCGACCATGTCCAGTGCCATGAAGCACATCGTCCGCTCCACGGGCATGTATCGCGGCGAAG---------TTC---------C---------------------GCCC---CCCAACTC---GTATCTGTATCCCCAGCCGCCGCCCCATGGCGTCCTGAAGGCCACCGGCGGTGTGGGCGTGGTCCCGGGAG------TG
dp5	XL_group1a:3383045-3383273 -		CTCGCCCCACACGGGAC---------CTGGCGGATC------------------GGGT------GGATCTGG------------------TCCCGGGGG------------------ATCCGAGCGGCACCATGTGCCACCGCCCT---CAATGTCCAGCGCCATGAAGCACATCGTCCGATCCACGGCCATGTATCGCGGAGAGGCGGTCTCCGTGCCGACGCTGGC---------------------GACG---GCCGGCTC---CTACATGTACC------AGCAGCCGCACGGCCCGCTCAAGGTGACGGGCGGACCCGAAGTGGGACCC------------
droWil2	scf2_1100000004909:3774937-3775126 -		TTCGCCGG-CATCGTCG---------CA-----GTC------------------AGC------------CTC------------------ATCGAGTTCCTCC---TCCG------TACCAGATCGTCATCACAATC------------------------CAATCAAGCACATTGTACGCTCCTCGCCCATCTATCGCAGCGAGGCTGTGGCCATGACCAGCCTGCC---------------------AGGA---CCTGGCCCAGGATATATATATCCAA---------CGCATGGAGTGCTGAAGGCCTCCTCCGGT---------------------------
droVir3	scaffold_12928:2239479-2239689 -		CTCGCCGCATACGGGCG---------CCGGC------------------------------------------------------------------------------G------CCGTTGATCGACATCATGGACCGCCGCCGA---CATTGGGCGGCGCCATGAAGCACATTGTGCGCACGGCGCCCATGTATAGATCCGAGGCGGGACCCGCATTGTCGGTGCC------CGGCGGACCGCCCAATGCG---CCCAGCTA---TTTGATGTACC------AGCAGCTTCATGGCGTG---------------------GGCGTGGGCGTAGGCGTGGTCAAG
droMoj3	scaffold_6473:14016006-14016219 -		CTCGCCGCACACGCATC---------CGCATGCGCA------------------CA---------GCTCGGT------------------GGCGA------------GCT------CTGTGGAGCGGCACCATGGCCCGCCGCCGCCAAGCATGGGCGGTGCGATGAAGCATATTGTGCGCACCTCGTCCATGTACCGGGGCGATGCCTCGA---TGCCCAGTTTGTCGGTGTCGGGTGCTCCGCCGCCCGCC---TCCAGCTA---TTTGACCTACC------AGCAGCTGCACGGCG---------------------------------------------TG
droGri2	scaffold_15203:5107764-5108010 +		TTCGCCGCACCGGGGCGTCAGTC---CGGCCGCAGCGGCAGCAGCAGCA---GCCGCCGCCG---------------------------------------TT---GGCA------TCGTTGAGCGTCATCATGGACCACCGCCGCCGATGACCAGCAGCTCCATGAAGCACATTGTGCGAACGGCGCCCATGTACCGTGGCGAGACGGTG---GTGCCAGCATTGTCTGTGCAGGGTGCACCGCCTCCGACA---TCCAATTA---TCTAACGTATC------AGCAGCTGCATGGCG---------------------TTGGTGTGGT---CAAGG------CG

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droSim2

CTCGCCGCACACGGGAG---------CCGGAGGATC------------------TAGCTCGTCTAGCTCTGG------------------ATCGGGTGGACCG---TCCT------CCTCGGACCGACATCATGGCCCACCACCGCCGACCATGT------CGATGAAGCACATTGTACGCTCTGGTGGCATGTATCGCGGTGACACCGTAACCGTTCCTTCGCTGGC---------------------TGCA---CCCAGTTC---GTATCTGTATCCCA---CACCGCCGCACGGAGTGGTCAAGGCCATCGGCGGCGGTGGCGTGGTGCCCGGTG------TG	Size	Hit Count	Total Norm	Total	M023 Head	M024 Male-body	M025 Embryo	M053 Female-body	O001 Testis	O002 Head	SRR553485 Ovary	SRR553486 Ovary	SRR553487 Ovary	SRR553488 Ovary	SRR618934 Ovary	V044 Embryo	SRR902008 Ovary	SRR902009 Testis	SRR1275487 Male larvae	SRR1275485 Male prepupae	SRR1275483 Male prepupae
.....................................................................................................................................................................................................................................................................................................ACGGAGTGGTCAAGGCCATCG................................	21	1	2.00	2	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0
......................................................................................................................................................CATGT------CGATGAAGCACATTGTACG......................................................................................................................................................................	24	1	1.00	1	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0
...........................................................................................................................................................................................GGCATGTATCGCGGTGAC.............................................................................................................................................	18	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0
.............................................................................................................................................................................................................................................................................................................GTCAAGGCCATCGGCGGCGGT........................	21	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0
.......................................................AGCTCGTCTAGCTCTGG------------------AT..............................................................................................................................................................................................................................................................	19	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0
..........................................................................................................................................................................................TGGCATGTATCGCGGTGACACCGTA.......................................................................................................................................	25	1	1.00	1	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0
.............................................................................................................................................................................................................ACCGTAACCGTTCCTTCGCTG........................................................................................................................	21	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0
.......CACACGGGAG---------CCGGAGGATC------------------TAGCT...............................................................................................................................................................................................................................................................................................	25	1	1.00	1	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0
...................................................................................................................................................................................................................................................................................................GCACGGAGTGGTCAAGGCCATCGGCGGC...........................	28	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0
.......................................................AGCTCGTCTAGCTCTGG------------------ATCGGGTGGACC....................................................................................................................................................................................................................................................	29	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..CGCCGCACACGGGAG---------CCGGAG..........................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0

droSec2

CTCGCCGCACACGGGAG---------CCGGAGGATC------------------TAGCTCGTCTAGCTCTGG------------------ATCGGGTGGACCG---TCCT------CCTCGGACCGACATCATGGCCCACCACCGCCGACCATAT------CGATGAAGCACATTGTACGCTCTGGTGGCATGTATCGCGGTGACACCGTAACCGTTCCTTCGCTGGC---------------------TGCA---CCAAGTTC---GTATCTGTATCCCA---CACCGCCGCACGGAGTGGTCAAGGCCATCGGCGGCGGTGGCGTGGTGCCCGGTG------TG	Size	Hit Count	Total Norm	Total	M054 Female-body	V113 Male-body	V114 Embryo	V115 Head
...................................C------------------TAGCTCGTCTAGCTCTGG------------------ATCGGGT.........................................................................................................................................................................................................................................................	26	1	3.00	3	0	2	1	0
.......................................................AGCTCGTCTAGCTCTGG------------------ATCGGGT.........................................................................................................................................................................................................................................................	24	1	1.00	1	0	0	1	0
..............................................................................................................................................................................................................CCGTAACCGTTCCTTCGCTGGC---------------------T................................................................................................	23	1	1.00	1	0	1	0	0
.............................................................................................................................................................................................................................................................................ATCTGTATCCCA---CACCGCC.......................................................	19	1	1.00	1	0	0	1	0

dm3

TTCGCCGCACACGGGAG---------CCGGAGGATC------------------TAGCTCGTCTGGCTCTGG------------------ATCGGGTGGACCG---TCCT------CATCGGATCGACATCATGGCCCACCACCGCCGACCATGT------CGATGAAGCACATTGTACGCTCTGGTGGCATGTATCGCGGTGACACCGTAACTGTTCCATCGCTGGC---------------------TGCA---CCCAGTTC---GTATCTGTATCCCA-----------CACGGAGTG-TCAAGACCATTGGCGGCGGTGGCGTGGTGCCCGGTG------TG	Size	Hit Count	Total Norm	Total	GSM154618 Ovary	GSM154620 Ovary	GSM154621 Ovary	GSM154622 Ovary	GSM180328 Head	GSM180329 Mixed-body	GSM180330 Embryo	GSM180331 Embryo	GSM180332 Embryo	GSM180333 Embryo	GSM180334 Larvae	GSM180335 Disc	GSM180336 Pupae	GSM180337 S2	GSM231091 Ovary	GSM239041 Head	GSM239050 Head	GSM239051 S2	GSM239052 S2	GSM246084 Head	GSM266765 S2	GSM280082 Ovary	GSM280083 Ovary	GSM280084 Ovary	GSM280085 Testis	GSM280086 Ovary	GSM280087 S2-NP	GSM280088 S2-NP	GSM312995 Head	GSM313160 Head	GSM313161 Head	GSM313162 Head	GSM313163 Head	GSM313164 Head	GSM313165 Head	GSM343287 Embryo	GSM379050 Ovary	GSM379051 Ovary	GSM379052 Ovary	GSM379053 Ovary	GSM379054 Ovary	GSM379055 Ovary	GSM379056 Ovary	GSM379057 Ovary	GSM379058 Ovary	GSM379059 Ovary	GSM379060 Ovary	GSM379061 Ovary	GSM379062 Ovary	GSM379063 Ovary	GSM379064 Ovary	GSM379065 Ovary	GSM379066 Ovary	GSM379067 Ovary	GSM424739 S2	GSM424740 S2	GSM424741 S2	GSM467729 Female-body	GSM467730 Female-body	GSM467731 Female-body	M002 CS	M003 Male-body	M004 Male-body	M005 ML-DmD32	M006 ML-DmD32	M029 OSC	M030 OSC	M031 OSC	M032 S2-R+	M033 S2-R+	M034 S2-R+	M035 S2-R+	M036 S2-R+	M037 S2-R+	M038 S2-R+	M039 S2-R+	M048 S2-R+	OSS2 OSS	OSS6 OSS	OSS7 OSS	OSS8 OSS	S1 Head	S10 Embryo	S11 Embryo	S12 Embryo	S13 Embryo	S14 Embryo	S15 S2-GMR	S16 Kc	S17 Larvae	S18 Larvae	S19 Pupae	S2 Head	S20 Pupae	S21 Disc	S22 Head	S23 Female-body	S24 Male-body	S25 Larvae	S26 Larvae	S27 Pupae	S28 Pupae	S29 Head	S3 Male-body	S30 S2-GMR	S31 S2	S32 S2	S33 Kc	S34 Kc	S35 Kc	S4 Female-body	S5 Disc	S6 Embryo	S7 Embryo	S8 S2	S9 Embryo	SRR001337 Head	SRR001338 Head	SRR001339 Head	SRR001340 Head	SRR001341 Head	SRR001342 Head	SRR001343 Head	SRR001344 Head	SRR001345 Head	SRR001346 Head	SRR001347 Head	SRR001348 Head	SRR001349 Head	SRR001664 Head	SRR010951 Ovary	SRR010952 Ovary	SRR010953 Ovary	SRR010954 Ovary	SRR010955 Ovary	SRR010956 Ovary	SRR010957 Ovary	SRR010958 Ovary	SRR010959 Ovary	SRR010960 Ovary	SRR014268 Embryo	SRR014269 Embryo	SRR014270 Embryo	SRR014271 Embryo	SRR014272 Embryo	SRR014273 Ovary	SRR014274 Ovary	SRR014275 Ovary	SRR014276 Ovary	SRR014277 Ovary	SRR014278 Ovary	SRR014279 Ovary	SRR014280 Ovary	SRR014281 Ovary	SRR014282 Ovary	SRR023197 S2	SRR023399 S2	SRR023400 S2	SRR023402 S2	SRR023407 S2	SRR029028 S2	SRR029029 S2	SRR029030 S2	SRR029031 S2	SRR029032 S2	SRR029033 S2	SRR029608 Head	SRR029633 Head	SRR030145 gb3	SRR031692 Head	SRR031693 Head	SRR031694 Head	SRR031696 Head	SRR031697 Head	SRR031698 Head	SRR031699 Head	SRR031700 Head	SRR031701 Head	SRR031702 Head	SRR031703 Head	SRR031704 Head	SRR032092 S2	SRR032093 S2	SRR032094 S2	SRR032095 S2-NP	SRR032096 S2-NP	SRR032152 Head	SRR060643 Testis	SRR060644 Ovary	SRR060645 Testis	SRR060646 Ovary	SRR060647 Ovary	SRR060648 Ovary	SRR060649 Ovary	SRR060650 Testis	SRR060651 Ovary	SRR060652 Testis	SRR060653 Ovary	SRR065151 Ovary	SRR065152 Ovary	SRR065153 Ovary	SRR065154 Ovary	SRR065155 Ovary	SRR065800 Ovary	SRR065801 Ovary	SRR065802 Ovary	SRR065803 Ovary	SRR065804 Ovary	SRR065805 Ovary	SRR065806 Ovary	SRR065807 Ovary	SRR097865 S2-NP	SRR097866 S2-NP	SRR097867 S2-NP	SRR298536 Ovary	SRR298537 Ovary	SRR298711 Ovary	SRR298712 Ovary	SRR317110 Ovary	SRR317111 Ovary	SRR317112 Ovary	SRR317113 Ovary	SRR317114 Ovary	SRR317115 Ovary	SRR317116 Ovary	SRR317117 Ovary	SRR341115 S2	SRR341116 S2	SRR341117 S2	SRR341118 S2	SRR351332 Mixed-body	SRR351333 Mixed-body	T004 OSC	T005 CME-L1	V001 Embryo	V002 Embryo	V003 S2	V006 Female-body	V007 Head	V008 S2	V009 CME-L1	V010 ML-DmD20-C5	V011 Sg4	V012 Male-body	V013 Head	V014 DTT	V015 DreRFHV	V018 Kc167	V019 GM2	V020 S2-R+	V021 ML-DmD21	V022 ML-DmD32	V023 Head	V024 Kc167	V025 S2-R+	V026 1182-2H	V027 ML-DmD21	V028 CME-W1-Cl.8+	V029 ML-DmD9	V030 ML-DmD8	V031 GM2	V032 S1	V033 CME-W1-Cl.8+	V034 ML-DmD16-C3	V035 ML-DmD32	V036 ML-DmD20-C5	V037 S2	V038 S2	V063 Ovary	V0632 Ovary	V064 Ovary	V0642 Ovary	V065 Ovary	V0652 Ovary	V066 Ovary	V0662 Ovary	V067 S2-NP	V073 mbn2	V074 S3	V075 ML-DmBG1-C1	V076 ML-DmBG3-C2	V077 Head	V078 Head	V079 Head	V080 Head	V081 Embryo	V082 Embryo	V083 Male-body	V084 Female-body	V085 CME-W2	V086 Female-body	V088 Ovary	V0882 Ovary	V089 Ovary	V0892 Ovary	V090 Male-body	V091 fGS/OSS	V092 Male-body	V093 Male-body	V094 S2-R+	V095 S2-R+	V096 Ovary	V097 Ovary	V098 Ovary	V125 ML-DmD9	V126 CME-L1	V127 G2	V128 S3	V129 ML-DmBG1-C1	V130 ML-DmBG3-C2	V131 ML-DmD16-C3	V132 ML-DmD32	V133 Sg4	V134 ML-DmD8	V135 CME-W2	V136 Male-body	V137 Head	V138 Male-body	V139 Female-body	V140 Female-body	V141 Female-body	V142 Female-body	V144 OSC	V145 S2	V146 S1	V147 1182-2H	V148 mbn2	GSM1528798 follicle cells
.....CGCACACGGGAG---------CCGGAGGA........................................................................................................................................................................................................................................................................................................................	20	1	7.00	7	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	7	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.............................................................................................................T------CATCGGATCGACATCATGGC..................................................................................................................................................................................................................	21	1	5.00	5	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
......................................................................................................................................................................................................................GTTCCATCGCTGGC---------------------TGCA---CCC.......................................................................................	21	1	4.00	4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
............................................................................................................................................................................................GCATGTATCGCGGTGACACCG.........................................................................................................................................	21	1	4.00	4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.TCGCCGCACACGGGAG---------CCGGAGGATC------------------T...................................................................................................................................................................................................................................................................................................	27	1	4.00	4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.....................................................................................................................ATCGGATCGACATCATGGCCC................................................................................................................................................................................................................	21	1	3.00	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..................................................................................................................................................................GATGAAGCACATTGTACGCTCTGGTGGCAT..........................................................................................................................................................	30	1	3.00	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...............................................................................................................................................................................................TGTATCGCGGTGACACCGT........................................................................................................................................	19	1	3.00	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
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.................................................................GCTCTGG------------------ATCGGGTGGACCG---TCCT------......................................................................................................................................................................................................................................	24	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..........................................................................................................................................................................................................................................................GCA---CCCAGTTC---GTATCTGTATCCC..................................................................	24	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
............................................................................................................................................................................ATTGTACGCTCTGGTGGCATG.........................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................................................................................................................................CACCACCGCCGACCATGT------CGATGAAG.................................................................................................................................................................................	26	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..............................................................................................................................................................................................................................................................................................................................CGGTGGCGTGGTGCCCGG..........	18	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
............................................................................................................CT------CATCGGATCGACATCATGG...................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
................................................................................................................................................................................................................................................................CCCAGTTC---GTATCTGTATCCCA-----------CACG..................................................	26	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.....................................................................................................................................................................................................................TGTTCCATCGCTGGC---------------------TGCA---CCC.......................................................................................	22	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

droEre2

CTCGCCGCACACGGGCG---------CCGGCGGATC------------------TG---------GCTCCGT------------------GTCGGGCGGACCG---TCCT------CCGCGGACCGACACCATGGCCCGCCGCCGCCGACCATGC------CGATGAAGCACATCGTCCGCTCTGGCGGCATGTATCGCGGTGACACCGTGAGCGTGCCCTCGCTGGC---------------------TGCG---CCCAGCTC---CTATCTGTATCCCA---CACCGCCGCACGGCGTGCTCAAGGCCACCGGCGGCGTTGGCGGCGTCGGCGGCGTCGGCGTC	Size	Hit Count	Total Norm	Total	M055 Female-body	M057 Embryo	V060 Head	V040 Embryo	V107 Male-body	V108 Head	GSM1528801 follicle cells
..........................................................................................GTCGGGCGGACCG---TCCT------CC....................................................................................................................................................................................................................................	19	1	1.00	1	1	0	0	0	0	0	0
..............................................................................................................................................................................................................................................................................................................TCAAGGCCACCGGCGGCGTTG.......................	21	1	1.00	1	1	0	0	0	0	0	0
..........................................................................................................TCCT------CCGCGGACCGACACCATGGCC.................................................................................................................................................................................................................	25	1	1.00	1	1	0	0	0	0	0	0
.................................ATC------------------TG---------GCTCCGT------------------GTCGGGCGGACCG---TCC.............................................................................................................................................................................................................................................	28	1	1.00	1	0	1	0	0	0	0	0
............................................................................................................................................................................ATCGTCCGCTCTGGCGGCATGTATCGCG..................................................................................................................................................	28	1	1.00	1	1	0	0	0	0	0	0
...................................................................................................................................................GACCATGC------CGATGAAGCACATCGTC........................................................................................................................................................................	25	1	1.00	1	0	0	0	0	1	0	0
......................................................................................................................................................................AAGCACATCGTCCGCTCTGGCGG.............................................................................................................................................................	23	1	1.00	1	1	0	0	0	0	0	0
...............................................................................................................................CACCATGGCCCGCCGCCGCC.......................................................................................................................................................................................................	20	1	1.00	1	0	1	0	0	0	0	0
..............................................................................................................................................................................................................................................................................................................TCAAGGCCACCGGCGGCGTTGGCGG...................	25	1	1.00	1	0	0	0	0	0	0	1
...............................................................................................................................................................................................................................................................................................................................GTTGGCGGCGTCGGCGGCGTCGGC...	24	1	1.00	1	1	0	0	0	0	0	0
...............................................................................................................................................................................................TGTATCGCGGTGACACCGTG.......................................................................................................................................	20	1	1.00	1	1	0	0	0	0	0	0
................................GATC------------------TG---------GCTCCGT------------------GTCGGGC.........................................................................................................................................................................................................................................................	20	1	1.00	1	0	1	0	0	0	0	0
.............................................................................................................T------CCGCGGACCGACACCATGGCCCG...............................................................................................................................................................................................................	24	1	1.00	1	1	0	0	0	0	0	0

droYak3

CTCACCGCACACGGGTG---------CCGGTGGCTC------------------GGGCT---CCGGCTCCGG------------------CTCGATTGGAGCA---TCGTCCTCCGCCGCAGAACGACATCATGGCCCCCCGCCGCCGACCATGT------CGATGAAGCACATTGTACGCTCGGGCGGCATGTATCGCGGCGACACCGTCACCGTTCCATCGCTGGC---------------------CGCG---CCCAGTTC---GTATCTGTATCCCA---CACCGCCGCACGGCGTGCTCAAGGCCACCGGCGGCGTTGGCGTGGTGCCCGGCG------TG	Size	Hit Count	Total Norm	Total	M026 Head	M043 Female-body	M056 Embryo	V046 Embryo	V052 Head	V058 Head	V120 Male-body	SRR1275488 Male larvae	SRR1275486 Male prepupae	SRR1275484 Male prepupae	GSM1528802 follicle cells
........................................................................................................................................................................GCACATTGTACGCTCGGGC...............................................................................................................................................................	19	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0
.................................................................................................................................TCATGGCCCCCCGCCGCCGACCATGT------C........................................................................................................................................................................................	27	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0
........................................................................................................................................................................GCACATTGTACGCTCGGGCG..............................................................................................................................................................	20	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
.......................................................................................................................................................ATGT------CGATGAAGCACATTGTACGCTCG..................................................................................................................................................................	27	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
........................................................................................................................................................................GCACATTGTACGCTCGGGCGGC............................................................................................................................................................	22	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
.TCACCGCACACGGGTG---------CCGGTGGCTC------------------....................................................................................................................................................................................................................................................................................................	26	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0
.........................................................................................................................................................................................................CGACACCGTCACCGTTCCATCGCTGGC---------------------.................................................................................................	27	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
...................................................................................................................................ATGGCCCCCCGCCGCCGAC....................................................................................................................................................................................................	19	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
.............................................................................................................................................................................................................................................................................ATCTGTATCCCA---CACCGCCG......................................................	20	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
.................................................................................................................................................................................................................................................................................GTATCCCA---CACCGCCGCACGGCGTGC............................................	26	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
...................................................................................................................................................................................GCTCGGGCGGCATGTATCGC...................................................................................................................................................	20	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
................................................................................................................................................................................................GTATCGCGGCGACACCGTC.......................................................................................................................................	19	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
............................................................................................................................CGACATCATGGCCCCCCGCCGCC.......................................................................................................................................................................................................	23	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0
.......................................................................................................................................................................................................................TTCCATCGCTGGC---------------------CGCG---CCCAGTTC---G..............................................................................	26	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
.................................................................................................................................................................CGATGAAGCACATTGTACGCT....................................................................................................................................................................	21	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0

droEug1

TTCCCCGCATACGGGTG---------CCGGTGGTTT------------------GAGC------AGTTCTGG------------------ATCGGGTGGTCCACCCACAT------CATCGGATCGACATCATGGTCCACCACCACCGACCATGT------CAATGAAGCACATTGTCCGCTCGACGGGCATGTATCGTGGCGATGCCGTAACCGTTCCTTCACTCGC---------------------AGCG---CCCAGTTC---GTATCTGTATCCGA---CACCACCGCATGGAGTGCTTAAGGCCACCGGTGGTG--------------------------	Size	Hit Count	Total Norm	Total
No Reads

droBia1

CTCACCGCACACGGGGG---------CAGGAGGCTCGGC---------------GGGC------GGCTCCGG------------------AGCCGGAGGATCCTCCTCTT------CGGCGGATCGCCATCACGGGCCACCGCCGCCGACCATGT------CGATGAAGCATATTGTCCGCTCCACGGGCCTGTATCGCGGCGATACCGTCAATGTACCCTCGTTGGC---------------------GGCG---CCCAATTC---GTATCTGTATCCCA---CACCGCCGCACGGCGTGCTGAAGGCCCCCGGCGGCGTGGGCGTGGTGCCCGGCG------TC	Size	Hit Count	Total Norm	Total
No Reads

droTak1

CTCGCCGCACACGGGTG---GAT---------CATCGGCAGCAGCAGGGGGATCCAGC------AGCTCCTC------------------CTCCACTTCATCC---TCTT------CAGCGGATCGCCATCACGGACCGCCGCCGCCAACCATGT------CAATGAAGCATATTGTACGCTCAGCCAACATGTATCGCGGCGAAACAGTGAGTGTTCCCTCGCTGGC---------------------GGCG---CCCAGTTC---GTATCTGTATCCCA---CTCCACCGCATGGAGTTCTCAAGGCCACCGGTGGCGTGGGTGTGGTGCCCGGTG------TG	Size	Hit Count	Total Norm	Total
No Reads

droEle1

TTCACCGCACACCGG---------------TGGTTC------------------GAGTGGATCCGGATCGGGATCGGGATCGGGTGCGGG---TGCGGGCGTT---TCAT------CCACCGATCGCCATCATGGCCCACCACCGCCCAGCATGT------CGATGAAGCATATTGTACGCTCCACGAACATGTATCGCGGCGACACCGTTGGCGTCCCATCGCTGGC---------------------GGCACCACCCAACTC---CTATCTGTATCCCA---ATCCGCCGCATGGCGTGCTCAAGGCCGCCGGCGGTGTGGGCGTCGTGCCGGGCGT---TGTG	Size	Hit Count	Total Norm	Total
No Reads

droRho1

TTCGCCGCACACCGGCG---GAG---CCGGCGGATC------------------GGGTGGATCGGG---GGG------------------ATCTGTTGGACCC---TCGT------CCTCGGATCGTCACCATATCCCACAGCCACCGAGCATGT------CGATGAAGCACATGGTTCGCTCCACGAGCATGTATCGTGGCGATACCGTGACCGTGCCTTCGCTGGC---------------------AGTG---CCCAACTC---GTATCTGTATCCCA---CGACGACGCACGGCGTCCTCAAGACGACCGGTGGCGTGGGCGTGGTGCCCGGCG------TG	Size	Hit Count	Total Norm	Total
No Reads

droFic1

CTCACCGCACACGGGCG---GCTCCTCCGGCGGATC------------------GAGTGGATCCGGCTCGGTCACCGGATCGAGTG---GCTCGGGCGGTGCC---TCCT------CGTCGGATCGCCATCATGGTCCGCCGCCGCCCGGCATGT------CGATGAAGCACATCGTCCGCTCCACGACCATGTATCGTGGCGATGCCGTGAGTGTGCCCACGCTGGC---------------------GGGT---CCCAGTTC---GTATCTGTACTCCA---CTCCGCCGCACGGCGTGCTCAAGGCCACCGGCGGAGTGGGCGTGGTGCCCGGCG------TC	Size	Hit Count	Total Norm	Total
No Reads

droKik1

CTCACCGCACACGGCCG---------GAGGAGGA---------GGAGGA---GGCAGTTCCT---------------------------------------CA---ACCT------CCACAGAACGTCATCATGGACCGCCGCCACCCAGCATGT------CCATGAAGCACATAGTGCGCTCCACGGCCATGTATCGAGGGGATACCGTAACAGTGCCCTCGCTGAC---------------------AGCT---CCTAGCTC---GTATCTATACGCCC---AGCCGCCTCATGGCGTGCTCAAAGG-------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droAna3

TTCCCCGCACACGGGCG---GTC---CGGGCGGAGG------------------AG---------GCTCATC------------------GTCGAGTGTCTCG---TCGG---TGTCGTCGGACCGCCACCATGTGACGCC---ACCTATCATGTCCAGTGCCGTGAAGCACATTGTCCGCTCTACGGGCATGTATCGCGGGGAGG------------------TGCC---------------------GCCC---CCCAACTC---GTATCTGTATCCCCAGCCGCCGCCCCACGGCGTCCTGAAGGCCACCGGCGGAGTGGGCGTCGTTCCCGGAGTGGGTGTG	Size	Hit Count	Total Norm	Total	M044 Female-body	M058 Embryo	V039 Embryo	V055 Head	V105 Male-body	V106 Head
......................C---CGGGCGGAGG------------------AG---------GCTCATC------------------GTC.............................................................................................................................................................................................................................................................	23	1	2.00	2	0	2	0	0	0	0
..........................................................................................................................................................................................GGGCATGTATCGCGGGGA..............................................................................................................................................	18	1	2.00	2	0	0	0	0	0	2
..............................................................................................................................................................................................................................................................................................................TGAAGGCCACCGGCGGAGTGG.......................	21	1	1.00	1	0	0	0	0	0	1
......GCACACGGGCG---GTC---CGGGC...........................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	0	1	0	0	0	0
.................................................................................................................................................................................................................................................................................GTATCCCCAGCCGCCGCCCCA....................................................	21	1	1.00	1	0	0	0	0	0	1
.....................................................................................................................................................................................................GCGGGGAGG------------------TGCC---------------------GCCC---CCCAACTC---...............................................................................	25	1	1.00	1	0	1	0	0	0	0
....................................................................................................................................................ATCATGTCCAGTGCCGTGAAGCACATT...........................................................................................................................................................................	27	1	1.00	1	0	1	0	0	0	0
................G---GTC---CGGGCGGAGG------------------AG---------GCTC.....................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	1	0	0	0	0
...............................................................................................................................................................................................................................................................................................CGCCCCACGGCGTCCTGAAGGCCACC.................................	26	1	1.00	1	1	0	0	0	0	0

droBip1

TTCGCCGCACACGGGTC---------CGGGTGGTGG------------------CG---------GCTCCTC------------------GTCGATTGCCTCG---TCTG---TCTCCTCGGACCGCCACCATGTGGCGCC---ACCGACCATGTCCAGTGCCATGAAGCACATCGTCCGCTCCACGGGCATGTATCGCGGCGAAG---------TTC---------C---------------------GCCC---CCCAACTC---GTATCTGTATCCCCAGCCGCCGCCCCATGGCGTCCTGAAGGCCACCGGCGGTGTGGGCGTGGTCCCGGGAG------TG	Size	Hit Count	Total Norm	Total
No Reads

dp5

CTCGCCCCACACGGGAC---------CTGGCGGATC------------------GGGT------GGATCTGG------------------TCCCGGGGG------------------ATCCGAGCGGCACCATGTGCCACCGCCCT---CAATGTCCAGCGCCATGAAGCACATCGTCCGATCCACGGCCATGTATCGCGGAGAGGCGGTCTCCGTGCCGACGCTGGC---------------------GACG---GCCGGCTC---CTACATGTACC------AGCAGCCGCACGGCCCGCTCAAGGTGACGGGCGGACCCGAAGTGGGACCC------------	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
..........................................................................................................................................................................................................................................................................................................CCGCTCAAGGTGACGGGCGGACCCG.......................	25	1	2.00	2	0	0	0	0	0	0	2	0	0	0
....................................................................................................................................................................TGAAGCACATCGTCCGATCCACGGCCA...........................................................................................................................................................	27	1	2.00	2	0	0	0	0	0	0	0	2	0	0
...............................................................................................................................................................................................................................................................................ATGTACC------AGCAGCCGCACGGCCCGCTC..........................................	27	1	1.00	1	0	1	0	0	0	0	0	0	0	0
...................................................................................................................................................................ATGAAGCACATCGTCCGATCCACGGC.............................................................................................................................................................	26	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.......................................................................................................................................................................................CACGGCCATGTATCGCGGAGAGGC...........................................................................................................................................	24	1	1.00	1	0	0	0	0	0	0	0	0	0	1
............................................................................................................................................................................................................GGCGGTCTCCGTGCCGACGC..........................................................................................................................	20	1	1.00	1	0	1	0	0	0	0	0	0	0	0
................................GATC------------------GGGT------GGATCTGG------------------TCCCGGGGG------------------ATC..................................................................................................................................................................................................................................	28	1	1.00	1	0	0	1	0	0	0	0	0	0	0
..............GAC---------CTGGCGGATC------------------GGGT------G.........................................................................................................................................................................................................................................................................................	18	1	1.00	1	0	1	0	0	0	0	0	0	0	0
........................................................GT------GGATCTGG------------------TCCCGGGGG------------------.....................................................................................................................................................................................................................................	19	1	1.00	1	0	0	1	0	0	0	0	0	0	0
...............AC---------CTGGCGGATC------------------GGGT------GGAT......................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.............................GCGGATC------------------GGGT------GGATCTG...................................................................................................................................................................................................................................................................................	18	1	1.00	1	1	0	0	0	0	0	0	0	0	0
..........................................................................................................................................................................................GGCCATGTATCGCGGAGAGGCG..........................................................................................................................................	22	1	1.00	1	0	1	0	0	0	0	0	0	0	0

droWil2

TTCGCCGG-CATCGTCG---------CA-----GTC------------------AGC------------CTC------------------ATCGAGTTCCTCC---TCCG------TACCAGATCGTCATCACAATC------------------------CAATCAAGCACATTGTACGCTCCTCGCCCATCTATCGCAGCGAGGCTGTGGCCATGACCAGCCTGCC---------------------AGGA---CCTGGCCCAGGATATATATATCCAA---------CGCATGGAGTGCTGAAGGCCTCCTCCGGT---------------------------	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
...................................................................................................CTCC---TCCG------TACCAGATCGTCATCAC.....................................................................................................................................................................................................................	25	1	3.00	3	3	0	0	0	0
...GCCGG-CATCGTCG---------CA-----GTC------------------AGC------------CTC------------------ATC.............................................................................................................................................................................................................................................................	27	1	1.00	1	1	0	0	0	0
..........................................................................................................................................................................................GCCCATCTATCGCAGCGAGG............................................................................................................................................	20	1	1.00	1	0	0	0	1	0
.....................................................................................................................ACCAGATCGTCATCACAATC------------------------CAATCAAG.................................................................................................................................................................................	28	1	1.00	1	1	0	0	0	0
...................................................................................................................................................................................................................................................................................................GCATGGAGTGCTGAAGGC.....................................	18	1	1.00	1	0	0	0	1	0
.TCGCCGG-CATCGTCG---------CA-----GTC------------------AG..................................................................................................................................................................................................................................................................................................	22	1	1.00	1	1	0	0	0	0
............................................................................................................................................................................................CCATCTATCGCAGCGAGGCTGT........................................................................................................................................	22	1	1.00	1	0	1	0	0	0
...........................................................................................................CCG------TACCAGATCGTCATCACAATC------------------------C........................................................................................................................................................................................	25	1	1.00	1	0	0	0	0	1
.............GTCG---------CA-----GTC------------------AGC------------CTC------------------ATCGAGT.........................................................................................................................................................................................................................................................	22	1	1.00	1	0	1	0	0	0
.....................................................................................................CC---TCCG------TACCAGATCGTCATCACA....................................................................................................................................................................................................................	24	1	1.00	1	1	0	0	0	0

droVir3

CTCGCCGCATACGGGCG---------CCGGC------------------------------------------------------------------------------G------CCGTTGATCGACATCATGGACCGCCGCCGA---CATTGGGCGGCGCCATGAAGCACATTGTGCGCACGGCGCCCATGTATAGATCCGAGGCGGGACCCGCATTGTCGGTGCC------CGGCGGACCGCCCAATGCG---CCCAGCTA---TTTGATGTACC------AGCAGCTTCATGGCGTG---------------------GGCGTGGGCGTAGGCGTGGTCAAG	Size	Hit Count	Total Norm	Total	M027 Male-body	M028 Head	M047 Female-body	M061 Embryo	SRR060654 Ovary	SRR060655 Testis	SRR060656 Ovary	SRR060657 Testis	SRR060658 Ovary	SRR060659 Testis	SRR060660 Ovary	SRR060661 Embryo	SRR060662 Embryo	SRR060663 Embryo	SRR060664 Male-body	SRR060665 Female-body	SRR060666 Male-body	SRR060667 Female-body	SRR060668 Male-body	SRR060669 Female-body	SRR060670 Testis	SRR060671 Male-body	SRR060672 Female-body	SRR060673 Ovary	SRR060674 Ovary	SRR060675 Ovary	SRR060676 Ovary	SRR060677 Ovary	SRR060678 Testis	SRR060679 Testis	SRR060680 Testis	SRR060681 Testis	SRR060682 Embryo	SRR060683 Testis	SRR060684 Embryo	SRR060685 Embryo	SRR060686 Embryo	SRR060687 Embryo	SRR060688 Ovary	SRR060689 Testis	SRR1106712 embryo_0-2h	SRR1106713 embryo_0-2h	SRR1106714 embryo_2-4h	SRR1106715 embryo_4-6h	SRR1106716 embryo_4-6h	SRR1106717 embryo_6-8h	SRR1106718 embryo_6-8h	SRR1106719 embryo_8-10h	SRR1106720 embryo_8-10h	SRR1106721 embryo_10-12h	SRR1106722 embryo_10-12h	SRR1106723 embryo_12-14h	SRR1106724 embryo_12-14h	SRR1106725 embryo_14-16h	SRR1106726 embryo_14-16h	SRR1106727 larvae	SRR1106728 larvae	SRR1106729 mixed whole adult body	SRR1106730 embryo_16-30h	V047 Embryo	V053 Head	V116 Male-body	GSM1528803 follicle cells
.........................................................................................................................GATCGACATCATGGACCGCCGCC..........................................................................................................................................................................................................	23	1	2.00	2	0	0	1	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...................................................................................................................................................................................................................................................GCCCAATGCG---CCCAGCTA---TTTGATGT.......................................................................	26	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
......................................................................................................................................................................................................................GCATTGTCGGTGCC------CGGCG...........................................................................................................	19	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...............................................................................................................................................................................................................................................................................................AGCTTCATGGCGTG---------------------GGCGTGGGC...............	23	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.............................................................................................................G------CCGTTGATCGACATCAT.....................................................................................................................................................................................................................	18	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.....................................................................................................................CGTTGATCGACATCATGGAC.................................................................................................................................................................................................................	20	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
....................................................................................................................................................................................................................................................................................................CATGGCGTG---------------------GGCGTGGGC...............	18	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.................................................................................................................................................................................................................GACCCGCATTGTCGGTGCC------C...............................................................................................................	20	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.................................................................................................................................................................................................................................................................................GTACC------AGCAGCTTCATGGC................................................	19	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.......................................................................................................................................................................................................................................................................A---TTTGATGTACC------AGCAGCTTCAT...................................................	23	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.....................................................................................................................................................................................................................................................................CTA---TTTGATGTACC------AGCAGCTTCAT...................................................	25	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...................................................................................................................................................................................................................................................................................................TCATGGCGTG---------------------GGCGTGGGC...............	19	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
...............................................................................................................................................CGA---CATTGGGCGGCGCCATGA...................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
......................................................................................................................GTTGATCGACATCATGGACCGCCGC...........................................................................................................................................................................................................	25	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..........................................................................................................................................................................................GCCCATGTATAGATCCGAGGCGGGA.......................................................................................................................................	25	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
............................................................................................................................................................................................CCATGTATAGATCCGAGGCGGGACC.....................................................................................................................................	25	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.......................................................................................................................................................................................................................................................AATGCG---CCCAGCTA---TTTGAT.........................................................................	20	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.....................................................................................................................................................................GAAGCACATTGTGCGCACGGC................................................................................................................................................................	21	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.....................................................................................................................................................................................................................CGCATTGTCGGTGCC------CGGCG...........................................................................................................	20	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
....................................................................................................................................................................................................................................................................................................CATGGCGTG---------------------GGCGTGGGCGTAGGCGTGGT....	29	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1
...........................................................................................................................................................................................CCCATGTATAGATCCGAGGCGGGACC.....................................................................................................................................	26	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..................................................................................................................................................................................................ATAGATCCGAGGCGGGACC.....................................................................................................................................	19	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..........................................................................................................................................................................................................................................................................................................GTG---------------------GGCGTGGGCGTAGGC.........	18	3	0.33	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0

droMoj3

CTCGCCGCACACGCATC---------CGCATGCGCA------------------CA---------GCTCGGT------------------GGCGA------------GCT------CTGTGGAGCGGCACCATGGCCCGCCGCCGCCAAGCATGGGCGGTGCGATGAAGCATATTGTGCGCACCTCGTCCATGTACCGGGGCGATGCCTCGA---TGCCCAGTTTGTCGGTGTCGGGTGCTCCGCCGCCCGCC---TCCAGCTA---TTTGACCTACC------AGCAGCTGCACGGCG---------------------------------------------TG	Size	Hit Count	Total Norm	Total	M046 Female-body	V041 Embryo	V049 Head	V056 Head	M060 Embryo	V110 Male-body
.............................................................................................................................................................................................CATGTACCGGGGCGATGCCTC........................................................................................................................................	21	1	1.00	1	1	0	0	0	0	0
....................................................................................................................CTGTGGAGCGGCACCATGGCC.................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	1
............................................................................................................................................................................ATTGTGCGCACCTCGTCCAT..........................................................................................................................................................	20	1	1.00	1	1	0	0	0	0	0
...................................................................................................................................................................ATGAAGCATATTGTGCGCACCTCGTCCAT..........................................................................................................................................................	29	1	1.00	1	1	0	0	0	0	0
...............................................................................................................................................................................................................CTCGA---TGCCCAGTTTGTCGGTGTCG...............................................................................................................	25	1	1.00	1	1	0	0	0	0	0
...................................................................TCGGT------------------GGCGA------------GCT------CTGTGG................................................................................................................................................................................................................................	19	1	1.00	1	1	0	0	0	0	0
...................................................................................................................................................................ATGAAGCATATTGTGCGCA....................................................................................................................................................................	19	1	1.00	1	1	0	0	0	0	0
..................................................................................................................................................................................................................GA---TGCCCAGTTTGTCGGTGTCGG..............................................................................................................	23	1	1.00	1	1	0	0	0	0	0
..................................................................................................................................................................................................................GA---TGCCCAGTTTGTCGGTGTCGGGTGCT.........................................................................................................	28	1	1.00	1	1	0	0	0	0	0

droGri2

TTCGCCGCACCGGGGCGTCAGTC---CGGCCGCAGCGGCAGCAGCAGCA---GCCGCCGCCG---------------------------------------TT---GGCA------TCGTTGAGCGTCATCATGGACCACCGCCGCCGATGACCAGCAGCTCCATGAAGCACATTGTGCGAACGGCGCCCATGTACCGTGGCGAGACGGTG---GTGCCAGCATTGTCTGTGCAGGGTGCACCGCCTCCGACA---TCCAATTA---TCTAACGTATC------AGCAGCTGCATGGCG---------------------TTGGTGTGGT---CAAGG------CG	Size	Hit Count	Total Norm	Total	M041 Female-body	V109 Male-body
............................................................................................................................................CGCCGCCGATGACCAGCAGCTCC.......................................................................................................................................................................................	23	1	1.00	1	1	0
.............................................................................................................A------TCGTTGAGCGTCATCATGGACCACCGCC..........................................................................................................................................................................................................	29	1	1.00	1	0	1
.............................................................................................................................................................................................................................................................................................GCAGCTGCATGGCG---------------------TTGGTGTGG.................	23	1	1.00	1	0	1
.........................................................................................................................................................................................................................CCAGCATTGTCTGTGCAGGGTGCA.........................................................................................................	24	1	1.00	1	0	1

dsi_13210

x:11941170-11941319 -

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-9.3	-9.1	-9.0