dpe_98

CAAGTGATCGAAGACAGTTTAATAAAGAAAATATTCAATTTGCATTTGGCTTTAGAAACACAAAGCATGTGTGGTATTTTAACTCATACATATCTTGTGTTTCTAGATCAAATGTGAGTACTCGATGAATTCCGAATTGAGAAGTCCCAGATTTAAGTT *********************************..((((((((((((.(((((((((((((...(((((((((......)).)))))))...)))))))))))))))))))))))))....**********************************	Read size	# Mismatch	Hit Count	Total Norm	Total	V111 male body	M042 female body	V042 embryo	M021 embryo	V050 head
......................................................................................TACATATCTTGTGTTTCTAGAT...................................................	22	0	2	29.50	59	42	9	3	3	2
..................................................TTTAGAAACACAAAGCATGTGT.......................................................................................	22	0	8	8.88	71	70	1	0	0	0
......................................................................................TACATATCTTGTGTTTCTAGA....................................................	21	0	2	5.50	11	8	2	1	0	0
......................................................................................TACATATCTTGTGTTTCTAG.....................................................	20	0	2	4.00	8	3	5	0	0	0
......................................................................................TACATATCTTGTGTTTCTAGATT..................................................	23	1	2	1.50	3	1	2	0	0	0
..................................................TTTAGAAACACAAAGCATGTGTGT.....................................................................................	24	1	8	1.50	12	12	0	0	0	0
...................................................TTAGAAACACAAAGCATGTGT.......................................................................................	21	0	8	0.63	5	5	0	0	0	0
......................................................................................TACATATCTTGTGTTTCTAGATCCA................................................	25	1	2	0.50	1	0	1	0	0	0
......................................................................................TACATATCGTGTGTTTCTAG.....................................................	20	1	2	0.50	1	0	1	0	0	0
......................................................................................TACATATCTTGTGTTTCTAGATCT.................................................	24	1	2	0.50	1	0	1	0	0	0
......................................................................................TACATATCTTGTGTTTCTA......................................................	19	0	2	0.50	1	0	1	0	0	0
......................................................................................TACATATCTTGTGTTTCTAGAAAAA................................................	25	2	2	0.50	1	0	1	0	0	0
......................................................................................TACATATCTTGCGTTTCTAGA....................................................	21	1	2	0.50	1	0	0	0	0	1
.......................................................................................ACATATCTTGTGTTTCTAGA....................................................	20	0	2	0.50	1	0	1	0	0	0
......................................................................................AACATATCTTGTGTTTCTAGAT...................................................	22	1	2	0.50	1	0	1	0	0	0
..................................................TTTAGAAACACAAAGCATGT.........................................................................................	20	0	8	0.50	4	4	0	0	0	0
.......................................................................................ACATATCTTGTGTTTCTAGATC..................................................	22	0	2	0.50	1	0	1	0	0	0
......................................................................................TACATATCTTGTGTTTCTAGACT..................................................	23	2	2	0.50	1	1	0	0	0	0
...................................................TTAGAAACACAAAGCATGTGTGT.....................................................................................	23	1	8	0.25	2	2	0	0	0	0
..................................................TTTAGAAACACAAAGCATGTGTG......................................................................................	23	0	8	0.25	2	2	0	0	0	0
...................................................TTAGAAACACAAAGCATGTGTGTTT...................................................................................	25	2	8	0.13	1	1	0	0	0	0
..................................................TTTAGAAACACAAAGCATGTGTGGT....................................................................................	25	0	8	0.13	1	1	0	0	0	0
..................................................TTTAAAAACACAAAGCATGTGT.......................................................................................	22	1	8	0.13	1	1	0	0	0	0
..................................................TTTAGAAACACAAAGCATGTGTGG.....................................................................................	24	0	8	0.13	1	1	0	0	0	0
..................................................TCTAGAAACACAAAGCATGTGT.......................................................................................	22	1	8	0.13	1	1	0	0	0	0
..................................................TTTAGAAACACAAAGCATGTGTC......................................................................................	23	1	8	0.13	1	1	0	0	0	0
...................................................TTAGAAACACAAAGCATGTG........................................................................................	20	0	8	0.13	1	1	0	0	0	0
..................................................TTTAGAAACACAAAGCATGTGC.......................................................................................	22	1	8	0.13	1	1	0	0	0	0
..................................................TTTAGAAACACAAAGCATG..........................................................................................	19	0	8	0.13	1	1	0	0	0	0
...................................................TTAGAAACACAAAGCATGT.........................................................................................	19	0	8	0.13	1	1	0	0	0	0
..................................................TTTAGAAACACAAAGCATGTT........................................................................................	21	1	8	0.13	1	1	0	0	0	0
..................................................TTTAGAAACACAAAGCATGTGTCCT....................................................................................	25	2	8	0.13	1	1	0	0	0	0

GTTCACTAGCTTCTGTCAAATTATTTCTTTTATAAGTTAAACGTAAACCGAAATCTTTGTGTTTCGTACACACCATAAAATTGAGTATGTATAGAACACAAAGATCTAGTTTACACTCATGAGCTACTTAAGGCTTAACTCTTCAGGGTCTAAATTCAA **********************************..((((((((((((.(((((((((((((...(((((((((......)).)))))))...)))))))))))))))))))))))))....*********************************	Read size	# Mismatch	Hit Count	Total Norm	Total	V057 head
.......................................................................ACCAAAAAATTGAGTATAT.....................................................................	19	2	6	0.17	1	1
.......................................................................ACCATCAAATTCAGCATGT.....................................................................	19	3	15	0.07	1	1

Species	Coordinate	ID	Type	Alignment
droPer2	scaffold_10:2581141-2581299 +	dpe_98	confident	CAAGTGATCGAAGACAGTTTAATAAAGAAAATATTCAATTTGCATTTGGCTTTAGAAACACAAAGCATGTGTGGTATTTTAACTCATACATATCTTGTGTTTC-TAGATCAAATGTGAGTACTCGATGAATTCCGAATTGAGAAGTCCCAGATTTAAGTT
dp5	4_group4:3552282-3552441 +	dps-mir-2566a-1	Known_mirbase	CAAGTGATCGAAGACAGTTTAATAAAGAAAATATTCAATTTGCATTTGGCTTTAGAAACACAAAGCATGTGTGGTATTTTAACTCATACATAACTTGTGTCTCTTAGATCAAATGTGAGTACTCCACGAATTCCCAATTGAGAAGTCCCAGATTTAAGTT

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droPer2

CAAGTGATCGAAGACAGTTTAATAAAGAAAATATTCAATTTGCATTTGGCTTTAGAAACACAAAGCATGTGTGGTATTTTAACTCATACATATCTTGTGTTTC-TAGATCAAATGTGAGTACTCGATGAATTCCGAATTGAGAAGTCCCAGATTTAAGTT	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
......................................................................................TACATATCTTGTGTTTC-TAGAT...................................................	22	2	29.50	59	3	9	3	2	0	42
..................................................TTTAGAAACACAAAGCATGTGT........................................................................................	22	8	8.88	71	0	1	0	0	0	70
......................................................................................TACATATCTTGTGTTTC-TAGA....................................................	21	2	5.50	11	0	2	1	0	0	8
......................................................................................TACATATCTTGTGTTTC-TAG.....................................................	20	2	4.00	8	0	5	0	0	0	3
......................................................................................TACATATCTTGTGTTTC-TAGATT..................................................	23	2	1.50	3	0	2	0	0	0	1
..................................................TTTAGAAACACAAAGCATGTGTGT......................................................................................	24	8	1.50	12	0	0	0	0	0	12
...................................................TTAGAAACACAAAGCATGTGT........................................................................................	21	8	0.63	5	0	0	0	0	0	5
......................................................................................TACATATCTTGTGTTTC-TAGATCT.................................................	24	2	0.50	1	0	1	0	0	0	0
......................................................................................TACATATCTTGTGTTTC-TA......................................................	19	2	0.50	1	0	1	0	0	0	0
.......................................................................................ACATATCTTGTGTTTC-TAGA....................................................	20	2	0.50	1	0	1	0	0	0	0
..................................................TTTAGAAACACAAAGCATGT..........................................................................................	20	8	0.50	4	0	0	0	0	0	4
.......................................................................................ACATATCTTGTGTTTC-TAGATC..................................................	22	2	0.50	1	0	1	0	0	0	0
......................................................................................TACATATCTTGTGTTTC-TAGACT..................................................	23	2	0.50	1	0	0	0	0	0	1
...................................................TTAGAAACACAAAGCATGTGTGT......................................................................................	23	8	0.25	2	0	0	0	0	0	2
..................................................TTTAGAAACACAAAGCATGTGTG.......................................................................................	23	8	0.25	2	0	0	0	0	0	2
..................................................TTTAGAAACACAAAGCATGTGTGGT.....................................................................................	25	8	0.13	1	0	0	0	0	0	1
..................................................TTTAGAAACACAAAGCATGTGTGG......................................................................................	24	8	0.13	1	0	0	0	0	0	1
..................................................TTTAGAAACACAAAGCATGTGTC.......................................................................................	23	8	0.13	1	0	0	0	0	0	1
...................................................TTAGAAACACAAAGCATGTG.........................................................................................	20	8	0.13	1	0	0	0	0	0	1
..................................................TTTAGAAACACAAAGCATGTGC........................................................................................	22	8	0.13	1	0	0	0	0	0	1
..................................................TTTAGAAACACAAAGCATG...........................................................................................	19	8	0.13	1	0	0	0	0	0	1
...................................................TTAGAAACACAAAGCATGT..........................................................................................	19	8	0.13	1	0	0	0	0	0	1
..................................................TTTAGAAACACAAAGCATGTT.........................................................................................	21	8	0.13	1	0	0	0	0	0	1

dp5

CAAGTGATCGAAGACAGTTTAATAAAGAAAATATTCAATTTGCATTTGGCTTTAGAAACACAAAGCATGTGTGGTATTTTAACTCATACATAACTTGTGTCTCTTAGATCAAATGTGAGTACTCCACGAATTCCCAATTGAGAAGTCCCAGATTTAAGTT	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
......................................................................................TACATAACTTGTGTCTCTTAGA....................................................	22	2	369.50	739	0	26	0	0	0	0	4	0	707	2
......................................................................................TACATAACTTGTGTCTCTTAG.....................................................	21	2	277.00	554	0	21	0	0	0	5	16	4	508	0
......................................................................................TACATAACTTGTGTCTCTTAGAT...................................................	23	2	99.50	199	1	15	2	2	6	3	19	2	149	0
......................................................................................TACATAACTTGTGTCTCTTAGAA...................................................	23	2	75.50	151	0	1	0	0	0	1	3	0	146	0
......................................................................................TACATAACTTGTGTCTCTTAGATC..................................................	24	2	32.00	64	1	1	0	0	0	0	0	0	62	0
......................................................................................TACATAACTTGTGTCTCTTA......................................................	20	2	17.00	34	0	2	0	1	0	0	0	0	31	0
......................................................................................TACATAACTTGTGTCTCTTAGATCC.................................................	25	2	17.00	34	0	2	0	1	0	0	1	0	30	0
......................................................................................TACATAACTTGTGTCTCTTAGAAA..................................................	24	2	16.00	32	0	2	0	0	0	0	0	0	30	0
..................................................TTTAGAAACACAAAGCATGTGT........................................................................................	22	2	15.50	31	0	4	0	1	1	1	18	0	6	0
......................................................................................TACATAACTTGTGTCTCTTAGATT..................................................	24	2	14.50	29	0	3	0	0	0	1	1	0	24	0
......................................................................................TACATAACTTGTGTCTCTTAGATCCC................................................	26	2	9.00	18	1	0	0	0	0	0	1	0	16	0
......................................................................................TACATAACTTGTGTCTCTTAGATA..................................................	24	2	8.50	17	0	2	0	1	0	0	0	0	14	0
..................................................TTTAGAAACACAAAGCATGTG.........................................................................................	21	2	7.50	15	0	2	0	0	0	1	1	0	11	0
..................................................TTTAGAAACACAAAGCATGTGTGG......................................................................................	24	2	6.50	13	0	0	0	0	0	0	0	0	13	0
......................................................................................TACATAACTTGTGTCTCTTAA.....................................................	21	2	4.50	9	0	0	0	0	0	1	0	0	8	0
......................................................................................TACATAACTTGTGTCTCTTAGATTT.................................................	25	2	4.00	8	0	1	0	0	0	0	0	0	7	0
..................................................TTTAGAAACACAAAGCATGTGTG.......................................................................................	23	2	4.00	8	0	0	0	0	0	0	1	0	7	0
.....................................................................................ATACATAACTTGTGTCTCTTAGA....................................................	23	2	3.50	7	0	0	0	0	0	0	0	0	7	0
......................................................................................TACATAACTTGTGTCTCTTT......................................................	20	2	3.00	6	0	0	0	0	0	0	0	0	6	0
......................................................................................TACATAACTTGTGTCTCTTAGT....................................................	22	2	2.50	5	0	0	0	0	0	0	1	0	4	0
......................................................................................TACATAACTTGTGTCTCTTAGATCA.................................................	25	2	2.00	4	0	0	0	0	0	0	0	0	4	0
..................................................TTTAGAAACACAAAGCATGT..........................................................................................	20	2	2.00	4	0	0	0	0	0	0	2	0	2	0
.......................................................................................ACATAACTTGTGTCTCTTAGA....................................................	21	2	1.50	3	0	0	0	0	0	0	0	0	3	0
...................................................TTAGAAACACAAAGCATGTGTGGTAT...................................................................................	26	2	1.50	3	0	0	0	0	0	0	0	0	3	0
..................................................TTTAGAAACACAAAGCATGTGTT.......................................................................................	23	2	1.50	3	0	1	0	0	0	0	0	0	2	0
..................................................TTTAGAAACACAAAGCATGTGTGGTA....................................................................................	26	2	1.50	3	0	0	0	0	0	0	0	0	3	0
......................................................................................TACATAACTTGTGTCTCTTAGTCT..................................................	24	2	1.50	3	0	0	0	0	0	0	0	0	3	0
......................................................................................TACATAACTTGTGTCTCTTAGATCT.................................................	25	2	1.50	3	0	0	0	0	0	0	0	0	3	0
......................................................................................TACATAACTTGTGTCTCTTAGATTCC................................................	26	2	1.50	3	0	1	0	0	0	0	0	0	2	0
....................................................TAGAAACACAAAGCATGTGTGG......................................................................................	22	2	1.00	2	0	0	0	0	0	1	0	0	1	0
..................................................TTTAGAAACACAAAGCATGTGTGT......................................................................................	24	2	1.00	2	0	0	0	0	0	0	2	0	0	0
......................................................................................TACATAACTTGTGTCTCTTG......................................................	20	2	1.00	2	0	0	0	0	0	0	0	0	2	0
...............................................................................TAACTCATACATAACTTGTGTCTCTTAGA....................................................	29	2	1.00	2	0	0	0	0	0	0	0	0	2	0
......................................................................................TACATAACTTGTGTCTCTTAGATCCCC...............................................	27	2	1.00	2	0	0	0	0	0	0	0	0	2	0
.......................................................................................ACATAACTTGTGTCTCTTAG.....................................................	20	2	1.00	2	0	2	0	0	0	0	0	0	0	0
......................................................................................TACATAACTTGTGTCTCTTAGATCAA................................................	26	2	1.00	2	0	0	0	0	0	0	0	0	2	0
.......................................................................................ACATAACTTGTGTCTCTTAGAT...................................................	22	2	1.00	2	0	2	0	0	0	0	0	0	0	0
...............................................................................TAACTCATACATAACTTGTGTCTCT........................................................	25	2	1.00	2	0	0	0	0	0	0	0	0	2	0
..................................................TTTAGAAACACAAAGCATGTGTGTCT....................................................................................	26	2	0.50	1	0	1	0	0	0	0	0	0	0	0
......................................................................................TACATAACTTGTGTCTCTTAGATG..................................................	24	2	0.50	1	0	0	0	0	0	1	0	0	0	0
......................................................................................TACATAACTTGTGTCTCTTAGATAT.................................................	25	2	0.50	1	0	0	0	0	0	0	0	0	1	0
.........................................................................................ATAACTTGTGTCTCTTAGA....................................................	19	2	0.50	1	0	1	0	0	0	0	0	0	0	0
....................................................TAGAAACACAAAGCATGTGT........................................................................................	20	2	0.50	1	0	1	0	0	0	0	0	0	0	0
...............................................................................TAACTCATACATAACTTGTGTCT..........................................................	23	2	0.50	1	0	0	0	0	0	0	1	0	0	0
......................................................................................TACATAACTTGTGTCTCTTAGATCCCT...............................................	27	2	0.50	1	0	0	0	0	0	0	1	0	0	0
.....................................................................................ATACATAACTTGTGTCTCTTAG.....................................................	22	2	0.50	1	0	0	0	0	0	0	0	0	1	0
...................................................TTAGAAACACAAAGCATGTGTG.......................................................................................	22	2	0.50	1	0	1	0	0	0	0	0	0	0	0
......................................................................................TACATAACTTGTGTCTCTTAGG....................................................	22	2	0.50	1	0	0	0	0	0	0	0	0	1	0
..................................................TTTAGAAACACAAAGCATGTGTGTC.....................................................................................	25	2	0.50	1	0	0	0	0	0	0	0	0	1	0
.................................................CTTTAGAAACACAAAGCATGT..........................................................................................	21	2	0.50	1	0	1	0	0	0	0	0	0	0	0
..................................................TTTAGAAACACAAAGCATGTGTGGT.....................................................................................	25	2	0.50	1	0	0	0	0	0	0	0	0	1	0
......................................................................................TACATAACTTGTGTCTCTTAGATCG.................................................	25	2	0.50	1	0	1	0	0	0	0	0	0	0	0
.......................................................................................ACATAACTTGTGTCTCTTAGATA..................................................	23	2	0.50	1	0	1	0	0	0	0	0	0	0	0
...............................TATTCAATTTGCATTTGGC..............................................................................................................	19	2	0.50	1	0	1	0	0	0	0	0	0	0	0
......................................................................................TACATAACTTGTGTCTCTTAGAC...................................................	23	2	0.50	1	0	0	0	1	0	0	0	0	0	0
..................................................TTTAGAAACACAAAGCATGTGTGGTAT...................................................................................	27	2	0.50	1	0	0	0	0	0	0	0	0	1	0
...............................................................................TAACTCATACATAACTTGTGTCTCTTA......................................................	27	2	0.50	1	0	0	0	0	0	0	0	0	1	0
.............................................................................TTTAACTCATACATAACTTGTGTCTC.........................................................	26	2	0.50	1	0	0	0	0	0	0	0	0	1	0
....................................................TAGAAACACAAAGCATGTGTGGT.....................................................................................	23	2	0.50	1	0	0	0	0	0	0	1	0	0	0
...................................................TTAGAAACACAAAGCATGTGT........................................................................................	21	2	0.50	1	0	0	0	0	0	0	0	0	1	0
....................................................................................CATACATAACTTGTGTCTCTTAGAT...................................................	25	2	0.50	1	0	0	0	0	0	0	0	0	1	0
...................................................TTAGAAACACAAAGCATGTGTGG......................................................................................	23	2	0.50	1	0	1	0	0	0	0	0	0	0	0
..................................................TTTAGAAACACAAAGCATGTT.........................................................................................	21	2	0.50	1	0	0	0	0	0	0	0	0	1	0
..................................................TTTAGAAACACAAAGCATGTGTGGTATT..................................................................................	28	2	0.50	1	0	0	0	0	0	0	1	0	0	0
......................................................................................TACATAACTTGTGTCTCTTAGATCCT................................................	26	2	0.50	1	0	0	0	0	0	0	0	0	1	0
.............................................................................TTTAACTCATACATAACTTGTGTCTCT........................................................	27	2	0.50	1	0	0	0	0	0	0	0	0	1	0
..................................................TTTAGAAACACAAAGCATGTGTC.......................................................................................	23	2	0.50	1	0	0	0	0	0	0	0	0	1	0
...............................................................................................TGTGTCTCTTAGATCT.................................................	16	6	0.17	1	0	0	0	0	0	0	0	0	1	0

dpe_98

scaffold_10:2581191-2581249 +

Known Ortholog

Testes-restricted

intergenic

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads