dpe_97

TTACAAAATAATCAATAAAACAATCAATATCGATAATTTTATAATCGTCGATATAAAATCGTTGGTATTAATTTTTAATCAATAACACAATCAATATCGATAATTTTATAATCGTCGATATAAAATCGTTTGTATTAATTTTTAATCAATAACACAATCAATATC *********************************((((((((.(((.(((((((((((.(((((((((.(((...............))).))))))))).))))))).)))).)))))))))))...**********************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M021 embryo	M042 female body	V050 head	V057 head	V042 embryo	V111 male body
..................................................ATATAAAATCGTTGGTATTAAT.............................................................................................	22	0	1	105.00	105	84	17	0	3	1	0
..................................................ATATAAAATCGTTGGTATT................................................................................................	19	0	1	27.00	27	18	1	6	0	1	1
..................................................ATATAAAATCGTTGGTATTAA..............................................................................................	21	0	1	18.00	18	18	0	0	0	0	0
..................................................ATATAAAATCGTTGGTATTAAA.............................................................................................	22	1	1	10.00	10	10	0	0	0	0	0
..................................................ATATAAAATCGTTGGTATTA...............................................................................................	20	0	1	10.00	10	5	3	2	0	0	0
..................................................ATATAAAATCGTTGGTATTAAAA............................................................................................	23	2	1	8.00	8	5	3	0	0	0	0
..................................................ATATAAAATCGTTGGTATTAATT............................................................................................	23	0	1	7.00	7	7	0	0	0	0	0
..................................................ATATAAAATCGTTGGTATTAATAAA..........................................................................................	25	3	1	5.00	5	5	0	0	0	0	0
..................................................ATATAAAATCGTTGGTATTAATAA...........................................................................................	24	2	1	4.00	4	4	0	0	0	0	0
..................................................ATATAAAATCGTTGTTATT................................................................................................	19	1	1	3.00	3	3	0	0	0	0	0
...................................................TATAAAATCGTTGGTATTAAT.............................................................................................	21	0	1	3.00	3	0	3	0	0	0	0
..................................................ATATAAAATCGTTGGTATTAATA............................................................................................	23	1	1	3.00	3	3	0	0	0	0	0
........................CAATATCGATAATTTTATAATCG......................................................................................................................	23	0	3	2.67	8	0	5	1	1	1	0
...........................................................................................CAATATCGATAATTTTATAATCG...................................................	23	0	3	2.67	8	0	5	1	1	1	0
.................................................GATATAAAATCGTTGGTATT................................................................................................	20	0	1	2.00	2	2	0	0	0	0	0
..................................................ATATAAAATCGTTGTTATTAAT.............................................................................................	22	1	1	2.00	2	2	0	0	0	0	0
..................................................ATATAAAAGCGTTGGTATTAAT.............................................................................................	22	1	1	1.00	1	0	1	0	0	0	0
..................................................ATATAAAATCGTTGGTATTAATTAA..........................................................................................	25	2	1	1.00	1	1	0	0	0	0	0
..................................................ATATAAAAGCGTTGGTATTAATAA...........................................................................................	24	3	1	1.00	1	0	1	0	0	0	0
..................................................ATATAAAATTGTTGGTATT................................................................................................	19	1	1	1.00	1	0	0	0	1	0	0
..................................................ATATAAAATCGCTGGTATT................................................................................................	19	1	1	1.00	1	0	0	0	1	0	0
..................................................ATATAAAATCGTTGGTAT.................................................................................................	18	0	1	1.00	1	0	0	0	1	0	0
..................................................ATATAAAATCGTTGGGATT................................................................................................	19	1	1	1.00	1	0	0	0	1	0	0
..................................................ATATAAAATCGTGGGTATTAA..............................................................................................	21	1	1	1.00	1	1	0	0	0	0	0
..................................................ATATAAAATCGTTGGAATTAAT.............................................................................................	22	1	1	1.00	1	1	0	0	0	0	0
.....................................................................................................................ATATAAAATCGTTTGTATTAAT..........................	22	0	1	1.00	1	1	0	0	0	0	0
..................................................ATATAAAATCGTTGGAATTAAA.............................................................................................	22	2	1	1.00	1	1	0	0	0	0	0
.................................................TATATAAAATCGTTGGTATTAAT.............................................................................................	23	1	1	1.00	1	0	1	0	0	0	0
.................................................CATATAAAATCGTTGGTATTAA..............................................................................................	22	1	1	1.00	1	1	0	0	0	0	0
.....................................................................................................................ATATAAAATCGTTTGTATT.............................	19	0	1	1.00	1	0	0	0	1	0	0
..................................................CTATAAAATCGTTGGTATTAAT.............................................................................................	22	1	1	1.00	1	1	0	0	0	0	0
..........................................................................................TCAATATCGATAATTTTATAATCG...................................................	24	0	3	0.67	2	0	1	0	0	1	0
.......................TCAATATCGATAATTTTATAATCG......................................................................................................................	24	0	3	0.67	2	0	1	0	0	1	0
........................CAATATCGATAATTTTATAATC.......................................................................................................................	22	0	3	0.33	1	0	0	1	0	0	0
...........................................................................................CAATATCGATAATTTTATAATTG...................................................	23	1	3	0.33	1	0	1	0	0	0	0
............................................................................................AATATCGATAATTTTATAATCG...................................................	22	0	3	0.33	1	0	1	0	0	0	0
...........................................................................................CAATATCGATAATTTTATAATC....................................................	22	0	3	0.33	1	0	0	1	0	0	0
........................CAATATCGATAATTTTATAATTG......................................................................................................................	23	1	3	0.33	1	0	1	0	0	0	0
.........................AATATCGATAATTTTATAATCG......................................................................................................................	22	0	3	0.33	1	0	1	0	0	0	0
..................................................ATATAGAATCGTTGGTTTT................................................................................................	19	2	4	0.25	1	0	0	0	1	0	0
.....................................................................................................................ATATAAAATCGTGTGGATT.............................	19	2	9	0.11	1	0	0	0	1	0	0

TTACAAAATAATCAATAAAACAATCAATATCGATAATTTTATAATCGTCGATATAAAATCGTTGGTATTAATTTTTAATCAATAACACAATCAATATCGATAATTTTATAATCGTCGATATAAAATCGTTTGTATTAATTTTTAATCAATAACACAATCAATATC

***********************************((((((((.(((.(((((((((((.(((((((((.(((...............))).))))))))).))))))).)))).)))))))))))...************************************

Read size

# Mismatch

Hit Count

Total Norm

Total

M021

embryo

M042

female body

V050

head

V057

head

V042

embryo

V111

male body

..................................................ATATAAAATCGTTGGTATTAAT.............................................................................................

105.00

105

..................................................ATATAAAATCGTTGGTATT................................................................................................

27.00

..................................................ATATAAAATCGTTGGTATTAA..............................................................................................

18.00

..................................................ATATAAAATCGTTGGTATTAAA.............................................................................................

10.00

..................................................ATATAAAATCGTTGGTATTA...............................................................................................

10.00

..................................................ATATAAAATCGTTGGTATTAAAA............................................................................................

8.00

..................................................ATATAAAATCGTTGGTATTAATT............................................................................................

7.00

..................................................ATATAAAATCGTTGGTATTAATAAA..........................................................................................

5.00

..................................................ATATAAAATCGTTGGTATTAATAA...........................................................................................

4.00

..................................................ATATAAAATCGTTGTTATT................................................................................................

3.00

...................................................TATAAAATCGTTGGTATTAAT.............................................................................................

3.00

..................................................ATATAAAATCGTTGGTATTAATA............................................................................................

3.00

........................CAATATCGATAATTTTATAATCG......................................................................................................................

2.67

...........................................................................................CAATATCGATAATTTTATAATCG...................................................

2.67

.................................................GATATAAAATCGTTGGTATT................................................................................................

2.00

..................................................ATATAAAATCGTTGTTATTAAT.............................................................................................

2.00

..................................................ATATAAAAGCGTTGGTATTAAT.............................................................................................

1.00

..................................................ATATAAAATCGTTGGTATTAATTAA..........................................................................................

1.00

..................................................ATATAAAAGCGTTGGTATTAATAA...........................................................................................

1.00

..................................................ATATAAAATTGTTGGTATT................................................................................................

1.00

..................................................ATATAAAATCGCTGGTATT................................................................................................

1.00

..................................................ATATAAAATCGTTGGTAT.................................................................................................

1.00

..................................................ATATAAAATCGTTGGGATT................................................................................................

1.00

..................................................ATATAAAATCGTGGGTATTAA..............................................................................................

1.00

..................................................ATATAAAATCGTTGGAATTAAT.............................................................................................

1.00

.....................................................................................................................ATATAAAATCGTTTGTATTAAT..........................

1.00

..................................................ATATAAAATCGTTGGAATTAAA.............................................................................................

1.00

.................................................TATATAAAATCGTTGGTATTAAT.............................................................................................

1.00

.................................................CATATAAAATCGTTGGTATTAA..............................................................................................

1.00

.....................................................................................................................ATATAAAATCGTTTGTATT.............................

1.00

..................................................CTATAAAATCGTTGGTATTAAT.............................................................................................

1.00

..........................................................................................TCAATATCGATAATTTTATAATCG...................................................

0.67

.......................TCAATATCGATAATTTTATAATCG......................................................................................................................

0.67

........................CAATATCGATAATTTTATAATC.......................................................................................................................

0.33

...........................................................................................CAATATCGATAATTTTATAATTG...................................................

0.33

............................................................................................AATATCGATAATTTTATAATCG...................................................

0.33

...........................................................................................CAATATCGATAATTTTATAATC....................................................

0.33

........................CAATATCGATAATTTTATAATTG......................................................................................................................

0.33

.........................AATATCGATAATTTTATAATCG......................................................................................................................

0.33

..................................................ATATAGAATCGTTGGTTTT................................................................................................

0.25

.....................................................................................................................ATATAAAATCGTGTGGATT.............................

0.11

Species	Coordinate	ID	Type	Alignment
droPer2	scaffold_3:6409860-6410024 -	dpe_97	confident	TTACAAAATAATCAATAAAACAATCAATATCGATAATTTTATAATCGTCGATATAAAATCGTTGGTATTAATTTTTAATCAATAACACAATCAATATCGATAATTTTATAATCGTCGATATAAAATCGTTTGTATTAATTTTTAATCAATAACACAATCAATATC
dp5	2:23634820-23634984 -			TTACAAAATAATCAATAAAACAATCAATATCGATAATTTTATAATCATCGATATAAAATCGTTGGTAATAATTTTTAATCAATAACACAATCAATATCGATAATTGTATAATCATCGATATAAAATCGATGGTATTAATTTTTAATCAATAACACAATCAATATC

Species

Coordinate

Type

Alignment

droPer2

scaffold_3:6409860-6410024 -

dpe_97

confident

TTACAAAATAATCAATAAAACAATCAATATCGATAATTTTATAATCGTCGATATAAAATCGTTGGTATTAATTTTTAATCAATAACACAATCAATATCGATAATTTTATAATCGTCGATATAAAATCGTTTGTATTAATTTTTAATCAATAACACAATCAATATC

dp5

2:23634820-23634984 -

TTACAAAATAATCAATAAAACAATCAATATCGATAATTTTATAATCATCGATATAAAATCGTTGGTAATAATTTTTAATCAATAACACAATCAATATCGATAATTGTATAATCATCGATATAAAATCGATGGTATTAATTTTTAATCAATAACACAATCAATATC

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droPer2

TTACAAAATAATCAATAAAACAATCAATATCGATAATTTTATAATCGTCGATATAAAATCGTTGGTATTAATTTTTAATCAATAACACAATCAATATCGATAATTTTATAATCGTCGATATAAAATCGTTTGTATTAATTTTTAATCAATAACACAATCAATATC	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
..................................................ATATAAAATCGTTGGTATTAAT.............................................................................................	22	1	105.00	105	84	17	1	0	3	0
..................................................ATATAAAATCGTTGGTATT................................................................................................	19	1	27.00	27	18	1	1	6	0	1
..................................................ATATAAAATCGTTGGTATTAA..............................................................................................	21	1	18.00	18	18	0	0	0	0	0
..................................................ATATAAAATCGTTGGTATTAAA.............................................................................................	22	1	10.00	10	10	0	0	0	0	0
..................................................ATATAAAATCGTTGGTATTA...............................................................................................	20	1	10.00	10	5	3	0	2	0	0
..................................................ATATAAAATCGTTGGTATTAAAA............................................................................................	23	1	8.00	8	5	3	0	0	0	0
..................................................ATATAAAATCGTTGGTATTAATT............................................................................................	23	1	7.00	7	7	0	0	0	0	0
..................................................ATATAAAATCGTTGGTATTAATAAA..........................................................................................	25	1	5.00	5	5	0	0	0	0	0
..................................................ATATAAAATCGTTGGTATTAATAA...........................................................................................	24	1	4.00	4	4	0	0	0	0	0
...................................................TATAAAATCGTTGGTATTAAT.............................................................................................	21	1	3.00	3	0	3	0	0	0	0
..................................................ATATAAAATCGTTGGTATTAATA............................................................................................	23	1	3.00	3	3	0	0	0	0	0
........................CAATATCGATAATTTTATAATCG......................................................................................................................	23	3	2.67	8	0	5	1	1	1	0
...........................................................................................CAATATCGATAATTTTATAATCG...................................................	23	3	2.67	8	0	5	1	1	1	0
.................................................GATATAAAATCGTTGGTATT................................................................................................	20	1	2.00	2	2	0	0	0	0	0
..................................................ATATAAAATCGTTGGTATTAATTAA..........................................................................................	25	1	1.00	1	1	0	0	0	0	0
..................................................ATATAAAATCGTTGGTAT.................................................................................................	18	1	1.00	1	0	0	0	0	1	0
.....................................................................................................................ATATAAAATCGTTTGTATTAAT..........................	22	1	1.00	1	1	0	0	0	0	0
.....................................................................................................................ATATAAAATCGTTTGTATT.............................	19	1	1.00	1	0	0	0	0	1	0
..........................................................................................TCAATATCGATAATTTTATAATCG...................................................	24	3	0.67	2	0	1	1	0	0	0
.......................TCAATATCGATAATTTTATAATCG......................................................................................................................	24	3	0.67	2	0	1	1	0	0	0
........................CAATATCGATAATTTTATAATC.......................................................................................................................	22	3	0.33	1	0	0	0	1	0	0
............................................................................................AATATCGATAATTTTATAATCG...................................................	22	3	0.33	1	0	1	0	0	0	0
...........................................................................................CAATATCGATAATTTTATAATC....................................................	22	3	0.33	1	0	0	0	1	0	0
.........................AATATCGATAATTTTATAATCG......................................................................................................................	22	3	0.33	1	0	1	0	0	0	0

dp5

TTACAAAATAATCAATAAAACAATCAATATCGATAATTTTATAATCATCGATATAAAATCGTTGGTAATAATTTTTAATCAATAACACAATCAATATCGATAATTGTATAATCATCGATATAAAATCGATGGTATTAATTTTTAATCAATAACACAATCAATATC	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
..............................................ATCGATATAAAATCGTTGGTATA................................................................................................	23	2	0.50	1	0	1	0	0	0	0	0	0	0	0

dpe_97

scaffold_3:6409910-6409974 -

confident

Canonical miRNA

intergenic

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-21.5	-21.5	-21.5