dpe_832

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------################################################## GGTGCGCGCCGACCACAAAATTTTGTGCAATCGAAGATCACATTACTCGCCCACCGTCTGACAACAAGTACGACGCAAGTAAGCGTCAGCTGCTCAAAACCTTCGGCGAGTAAGAGGATTCAAAAATGAGACGACTGCTCCGGGGCGGAGACATGACTGGGAAGAAGAAATCTGAAATTCATCTTCACTTACGTCGCTTAGCACCTGCTAATAGAGTTGCCTTCGTCGATTCGTCCGCTGATTTCGATTTG ******************************************************************.........(((....(((..((((((............)))).))..)))..............)))*****************************************************************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M042 female body	M021 embryo	V042 embryo	V057 head	V111 male body
...............................................................................................................AAGAGGATTCAAAAATGAGACGACT...................................................................................................................	25	0	2	2.00	4	4	0	0	0	0
.................................................................................................................................................................................................................................TCGATTCGTCCGCTGATTTCGATT..	24	0	2	1.00	2	2	0	0	0	0
.......................................ACATTACTCGCCCACCGTCTGACAACAAG.......................................................................................................................................................................................	29	0	4	0.75	3	0	3	0	0	0
..........................................................................................TGCTCAAAACCTTCGGCGAGTAAGAG.......................................................................................................................................	26	0	2	0.50	1	1	0	0	0	0
........................................................................................................................................................................................GCACTTACGTCGCTTAGCACCTGCTA.........................................	26	1	3	0.33	1	1	0	0	0	0
................................................................................................................AGAGGATTCAAAAATGAGA........................................................................................................................	19	0	7	0.29	2	2	0	0	0	0
.......................................ACATTACTCGCCCACCGTCTGACA............................................................................................................................................................................................	24	0	4	0.25	1	1	0	0	0	0
.......................................ACATTACTCGCCCACCGTCTGACAACA.........................................................................................................................................................................................	27	0	4	0.25	1	1	0	0	0	0
.........................TGCAATCGAAGATCACATTACTCGCC........................................................................................................................................................................................................	26	0	4	0.25	1	1	0	0	0	0
....................................................................TACGACGCAAGTAAGCGTCAGCTGCTC............................................................................................................................................................	27	0	5	0.20	1	1	0	0	0	0
.................................................................................................................................................................................................................................ACGCTTCGTCCGCTGGTTTC......	20	3	5	0.20	1	0	0	1	0	0
.....................................................................................TCAGCTGCTCAAAACCTTCGGCGAG.............................................................................................................................................	25	0	6	0.17	1	1	0	0	0	0
...............................................................................................................AAGAGGATTCAAAAATGAGACGGC....................................................................................................................	24	1	6	0.17	1	1	0	0	0	0
................................................................................................................AGAGGATTCAAAAATGAGAC.......................................................................................................................	20	0	7	0.14	1	1	0	0	0	0
...........................................TACTCGCCCACCGTCTGACAACAAGTA.....................................................................................................................................................................................	27	0	7	0.14	1	1	0	0	0	0
........................................................................................................................................GCTCCGGGGCGGAGACATGACTGG...........................................................................................	24	0	7	0.14	1	1	0	0	0	0
...........................................TACTCGCCCACCGTCCGACAACAAG.......................................................................................................................................................................................	25	1	7	0.14	1	0	1	0	0	0
................................................................................................................AGAGGATTGAAAAATGAGACG......................................................................................................................	21	1	7	0.14	1	1	0	0	0	0
...........................................TACTCGCCCACCGTCTGACAACAAG.......................................................................................................................................................................................	25	0	7	0.14	1	1	0	0	0	0
................................................................................................................AGAGGATTCAAAAATGAGACG......................................................................................................................	21	0	7	0.14	1	1	0	0	0	0
.......................................................................................................................................................................................................................GTTGCCTTCGTCGATTCGTCCGCT............	24	0	8	0.13	1	1	0	0	0	0
.........................................................................................................................................................................................................................TGCCTTCGTCGATTCGTCCGC.............	21	0	9	0.11	1	0	0	1	0	0
.........................................................................................................................................................................................................................TGCCTTCGTCGATTCGTCCGT.............	21	1	9	0.11	1	0	0	0	0	1
.........................................................................................................................................................................................................................TGCCTTCGTCGATTCGTC................	18	0	9	0.11	1	0	1	0	0	0
.........................................................................................................................................................................................................................TGCCTTCGTCGATTCGTCCGCT............	22	0	9	0.11	1	0	1	0	0	0
.................................................................................................................................................................................................................................TCGATTCGTCCGCTCATTTCGAAA..	24	3	11	0.09	1	1	0	0	0	0
.............................................................................................................................................GGGGCGGAGAGCTGACCGGG..........................................................................................	20	3	18	0.06	1	0	0	0	1	0
.............................................................................................................................................TGGGCGGAGACCTGATTGGG..........................................................................................	20	3	18	0.06	1	0	0	0	1	0
...................................................................................................................................................................................................................TAGAGGTTCCTGCGTCGA......................	18	3	20	0.05	1	0	0	0	1	0
............................................................................................................................................CGGGGGGGAGAAATGTCTGG...........................................................................................	20	3	20	0.05	1	0	0	1	0	0

CCACGCGCGGCTGGTGTTTTAAAACACGTTAGCTTCTAGTGTAATGAGCGGGTGGCAGACTGTTGTTCATGCTGCGTTCATTCGCAGTCGACGAGTTTTGGAAGCCGCTCATTCTCCTAAGTTTTTACTCTGCTGACGAGGCCCCGCCTCTGTACTGACCCTTCTTCTTTAGACTTTAAGTAGAAGTGAATGCAGCGAATCGTGGACGATTATCTCAACGGAAGCAGCTAAGCAGGCGACTAAAGCTAAAC *****************************************************************************************************************.........(((....(((..((((((............)))).))..)))..............)))******************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M042 female body	V042 embryo	V057 head
...............................................................................................................................................................................................................GATTATCTCAACGGAAGCAGCTAAG...................	25	0	1	1.00	1	1	0	0
.................................................................................................TTGGAAGCCGCTCATTCTCCTAAG..................................................................................................................................	24	0	2	0.50	1	1	0	0
..................................................................................................TGGAAGCCGCTCATTCTCCTAAG..................................................................................................................................	23	0	2	0.50	1	1	0	0
.......................................................................................................................................................................................................................CAACGGAAGCAGCTAAGCAGGCGACTAA........	28	0	2	0.50	1	1	0	0
...............................GCTTCTAGTGTAATGAGCGGGT......................................................................................................................................................................................................	22	0	4	0.25	1	1	0	0
........................CACGTTAGCTTCTAGTGTAATGAGC..........................................................................................................................................................................................................	25	0	4	0.25	1	1	0	0
.........................ACGTTAGCTTCTAGTGTAATGAGC..........................................................................................................................................................................................................	24	0	4	0.25	1	1	0	0
...........TGCTGTTTTAAAACACGTTAGCTTCT......................................................................................................................................................................................................................	26	1	4	0.25	1	1	0	0
.............................TAGCTTCTAGTGTAATGAGCGGGT......................................................................................................................................................................................................	24	0	4	0.25	1	1	0	0
.........................................................................GCGTTCATTCGCAGTCGACGAGT...........................................................................................................................................................	23	0	5	0.20	1	1	0	0
......................................................GCATACTGTTGTTCATGCTGCGT..............................................................................................................................................................................	23	1	6	0.17	1	0	1	0
.................................................GGGTGGCAGACTGTTGTTCATG....................................................................................................................................................................................	22	0	7	0.14	1	1	0	0
................................................CGGGTGGCAGACTGTTGTTCATG....................................................................................................................................................................................	23	0	7	0.14	1	1	0	0
....................................................................................TAGGCGACAAGTTTTGGAAG...................................................................................................................................................	20	3	15	0.07	1	1	0	0
..............................................................................................GTCTTGGTAGCCGGTCATT..........................................................................................................................................	19	3	16	0.06	1	0	0	1
.......................................................................................................................AGGCTTGACTCTGCTGACG.................................................................................................................	19	3	20	0.05	1	0	0	1

Species	Coordinate	ID	Alignment
droPer2	scaffold_159:54073-54323 -	dpe_832	GGTGCGCGCCGACCACAAAAT-----------------------------------TTTGTGCAATCGAAGATCACATTACTCGCCCACCGTCTGACAACAAGTACGACGCAAGTAAGCGTCAGCTGCTCAAAACCTTCGGCGAGTAAGAGGATTCAAAAATGAGACGACTGCTCCGGGGCGGAGACATGACTGGGAAGAAGAAATCTGAAATTCATCTTCACTTACGTCGCTTAGCACCTGCT-----------------------------------AATAGAGTTGCCTTCGTCGATTCGTCCGCTGATTTCGATTTG
dp5	Unknown_group_42:13890-14152 +		GGTGCACGACGACCACAAATTTGACCAAAAA-----------------------ATTTCGTGCAATCGAAGATCTCATTACTCGCCCACCGTCTGACAACAAGTACGACGCAAGTAAGCGTCAGCTGCTCAAAACCTTCGGAGAGTCAGAGGATTCAGAAATGAGCCGACTGTTCCGGGGAGGAGACATGACTGGGAAGAAGCCATCTGAAATTTTGCTTCACTTACGTCGCTTAGCACCTGCT-----------------------------------AATGGAGTTGCCTTCGTCGATTTTTCCGCTCATTTCGGTTTG
droWil2	scf2_1100000004943:4099930-4100216 +		GTGC-ATATAGACGACGA---------------------------------GATCATCATTGCAGTAGAAGATTTGGTTTTACGACCACCTGCGACTGACAAATATCTTGCCATCAAGAATCGTCTGCTTGAGAAATTCGCCGAATCGGCTGAATCAAAATTTCGACGTCTACTTCACGGCGATGGAACATCTGGATCAAAACCATCCGAAATTCTGGCAAACATGCGACGCCTCGCTCCGAATCCGGCAAGTGAAAGCTTAATTCGCACACTCTTTCTTGCAGAGATGCCTCAGTCAATACGTCCAATGCTGACAGTGTG
droVir3	scaffold_13036:1056574-1056866 -		GGTG---------------------------TCGTTACACTTAAAGAAGGATGTCGCACTCACCATCGAGGATCTCATCACATATCCACCGGTGCATAACAAATACGGCACAT-AGCGAAACGGCTTTTCGAAACATTTGCGGAATCAGAGGACTCAAAATAAAAGAGACTTCTGCAAGGTCGCGACATGACCACCAAGAATCCGTCAGAAATCCTTACACAAATGCGTCGTTTAACACCCGCTAACGGATTCGAAGCCGTTAAGCGCACTATATTTATTGCCGAGCTGGCAATGTCGGTTCGTCCGCTGATTTCTATCTG
droFic1	scf7180000453899:15629-15896 +		ACTA-----------------------------------------------AGAGGTTTTGGCTGTGGAAGACTTAGTCATTCGACCACCTGACGAAAACAAGTATA------TAAAAAATCGCTTGTTACAAAAATATGCTCAGTCTCCAGAGTCGAAACTACGCCGACTTTTACAGGGTGGGGGTACATCTGGTTTGAAATTATCTGAAATCTTGAACAACATGCGACGACTTGCTCCGGATTCGGGCAGCGAGAACATTACCCTCACACTTTTCCTGGCAGAGATGCCGGCATCAATTCATCCTACCCTCACCGTCTG
droEle1	scf7180000488816:7490-7810 -		TAACAATGACGACGACAAATTTGATCACATCACCGTTAACTTGGATCCGGAGGTCGTTTTAGCCGTCGAGGATTTAGTCACGCATCCTCCAGTCGCAAACAAGTTTGACACGCTGAAAGCTCGCTTGCTCGACAAATTTGCAGAGTCAGCAGAATCAAAACTACGGCGTTTACTGCAAGGTGGAGGAACTTCTGGCCTGAAGCCCTCCCAAATTCTAGCTCACATGCGTCGGCTGGCACCGGAATCCAGCAGCGAACCAATAATCCGTACATTGTTTCTTTCTGAAATGCCTCAGAATATTCGTCCGATTTAAACCGTCTG
droRho1	scf7180000773056:5716-6016 +		CAACAACGATGACAACAAATTTGACTACATCACCGTAAATTTGGATCCAGACGTTGTTGTG--------------------ACACCCGCCAGCGGCTAACAAGTATGATACCCTAAAGTCCCGATTGCTCGATAAGTTTGCAGAGACAGCCGAATCTAAGCTACGCCGTCTACTTCAAGGAGGAGGAACTGCTGGTCTGAAGCCTTCGCAAATTCTGGCTCATATGCGTCGTTTGGCTCCTGAGACAAGCAGTGAGTCAATAATCCGCACACTGTTCCTCGCCGAGATGCCCCAGAACATTCGCCCTATTTTATCGATATG
droBia1	scf7180000301696:30489-30800 +		CAACAACGATGACGACAAATTTGACTACATCACCGTAAATTTGGATCCAAACGTTGTTTTGACTGTGGAGGATTTAGTCACTCAACCGCCAGCGGCCAACAAGAATGATACTTTAAAGTTTCGGTTGCTCGATAAGTTTGCGGAGTCAGCCGAATCTAAGCTACGCCGCCTACTTCAAGGAGGAGGAACTGCTGGTCTGAAGCCTTCGCAAATTCTGGCTCATATGCGTCGTCTGGCTCCTGAGTCGAGCAGCGAGTCGATAATCCGCACACTGTTCCTTGCCGAGATGCCCCAAAACATTCGCCCTATTTT---------
droTak1	scf7180000414658:11415-11734 +		CATCGACGACACCGACAAGTTCTACCTTGTGACTCTTCACCTGGAGGAGGGTGTAGTTCTGGCAGTGGAAGACTTAGTCATTCAACCACCCATCGAAAACAAGTACGTAGCAATTAAAAGTCGCTTACTACAAAAATTTGCCGAATCTCCAGAGTCGAAACTACGCCGACTTCTGCAGGGTGGAGGTACATCTGGTTTAAAACCATCTGAGATCCTGGCCAACATGCGTCGACTACCTCCGGACCCGGGCAGCGAGAACATCATTCTCACTCTTTTCCTGGCAGAGATACCGGCGTCGATTCGCCCCACTCTCACTGTGT-
droYak3	v2_chrUn_004:641310-641574 -		--------------------------------------------------------TTTGGGCTGTAGAGGATTTGGTCACTCGTCCACCGGCAGACAACAAGTACGCAGCAATCAAAACTCGCTTGCTGCAGAAGTTCGCTGAATCGCCCGAGTCAAAACTGCGGCGACTACTTCAGGGAGGAGGTGCCACCGGATTAAAGCCTTCGGAAATCTTGGCAAATATGCGACGCTTGGCCCCGGATCCTAGCAGCATGAGCATCATTCACACCCTATTCCTCGCTGAGATGCCAGCCTCCATTCGTCCGCTGCTCACCGTATG
droEre2	scaffold_4732:162621-162924 +		TGTCGACGACAATGGTGA--------------CACGGCATTTTGA---ATATGTGATTTTGGTTGTGGATGGTTTGGTCACTCGTCCATCGGCAGATAACAAGTAAGCAGCAATCAAAACTCGCTTGCTGCAGAAGGTCGCTGAGTCGCCCGAATCAAAACTGCGACGACTACTTCAAGGAGGAGGTGCCACCGGATTAATGCCTTCGAAAATCTTGGAAAATATGCGACGCTCGGCCCCTGATCCTGGCAGCGAAAGCATCATTCACACCCTATTCCTCGCTGAGATGTCAGCCTCCATTCGTCCGCTGCACACCGTATG

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droPer2

GGTGCGCGCCGACCACAAAAT-----------------------------------TTTGTGCAATCGAAGATCACATTACTCGCCCACCGTCTGACAACAAGTACGACGCAAGTAAGCGTCAGCTGCTCAAAACCTTCGGCGAGTAAGAGGATTCAAAAATGAGACGACTGCTCCGGGGCGGAGACATGACTGGGAAGAAGAAATCTGAAATTCATCTTCACTTACGTCGCTTAGCACCTGCT-----------------------------------AATAGAGTTGCCTTCGTCGATTCGTCCGCTGATTTCGATTTG	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
..................................................................................................................................................AAGAGGATTCAAAAATGAGACGACT......................................................................................................................................................	25	2	2.00	4	0	4	0	0	0	0
.......................................................................................................................................................................................................................................................................................................TCGATTCGTCCGCTGATTTCGATT..	24	2	1.00	2	0	2	0	0	0	0
..........................................................................ACATTACTCGCCCACCGTCTGACAACAAG..........................................................................................................................................................................................................................	29	4	0.75	3	3	0	0	0	0	0
.............................................................................................................................TGCTCAAAACCTTCGGCGAGTAAGAG..........................................................................................................................................................................	26	2	0.50	1	0	1	0	0	0	0
...................................................................................................................................................AGAGGATTCAAAAATGAGA...........................................................................................................................................................	19	7	0.29	2	0	2	0	0	0	0
..........................................................................ACATTACTCGCCCACCGTCTGACA...............................................................................................................................................................................................................................	24	4	0.25	1	0	1	0	0	0	0
..........................................................................ACATTACTCGCCCACCGTCTGACAACA............................................................................................................................................................................................................................	27	4	0.25	1	0	1	0	0	0	0
............................................................TGCAATCGAAGATCACATTACTCGCC...........................................................................................................................................................................................................................................	26	4	0.25	1	0	1	0	0	0	0
.......................................................................................................TACGACGCAAGTAAGCGTCAGCTGCTC...............................................................................................................................................................................................	27	5	0.20	1	0	1	0	0	0	0
........................................................................................................................TCAGCTGCTCAAAACCTTCGGCGAG................................................................................................................................................................................	25	6	0.17	1	0	1	0	0	0	0
...................................................................................................................................................AGAGGATTCAAAAATGAGAC..........................................................................................................................................................	20	7	0.14	1	0	1	0	0	0	0
..............................................................................TACTCGCCCACCGTCTGACAACAAGTA........................................................................................................................................................................................................................	27	7	0.14	1	0	1	0	0	0	0
...........................................................................................................................................................................GCTCCGGGGCGGAGACATGACTGG..............................................................................................................................	24	7	0.14	1	0	1	0	0	0	0
..............................................................................TACTCGCCCACCGTCTGACAACAAG..........................................................................................................................................................................................................................	25	7	0.14	1	0	1	0	0	0	0
...................................................................................................................................................AGAGGATTCAAAAATGAGACG.........................................................................................................................................................	21	7	0.14	1	0	1	0	0	0	0
.............................................................................................................................................................................................................................................................................................GTTGCCTTCGTCGATTCGTCCGCT............	24	8	0.13	1	0	1	0	0	0	0
...............................................................................................................................................................................................................................................................................................TGCCTTCGTCGATTCGTCCGC.............	21	9	0.11	1	0	0	1	0	0	0
...............................................................................................................................................................................................................................................................................................TGCCTTCGTCGATTCGTCCGT.............	21	9	0.11	1	0	0	0	0	0	1
...............................................................................................................................................................................................................................................................................................TGCCTTCGTCGATTCGTC................	18	9	0.11	1	1	0	0	0	0	0
...............................................................................................................................................................................................................................................................................................TGCCTTCGTCGATTCGTCCGCT............	22	9	0.11	1	1	0	0	0	0	0

dp5

GGTGCACGACGACCACAAATTTGACCAAAAA-----------------------ATTTCGTGCAATCGAAGATCTCATTACTCGCCCACCGTCTGACAACAAGTACGACGCAAGTAAGCGTCAGCTGCTCAAAACCTTCGGAGAGTCAGAGGATTCAGAAATGAGCCGACTGTTCCGGGGAGGAGACATGACTGGGAAGAAGCCATCTGAAATTTTGCTTCACTTACGTCGCTTAGCACCTGCT-----------------------------------AATGGAGTTGCCTTCGTCGATTTTTCCGCTCATTTCGGTTTG	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
.......................................................................................................TACGACGCAAGTAAGCGTCAGCTGC.................................................................................................................................................................................................	25	1	2.00	2	0	2	0	0	0	0	0	0	0	0
.............................................................................................................................TGCTCAAAACCTTCGGAGAGTCAGA...........................................................................................................................................................................	25	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.........................................................................................................................................TCGGAGAGTCAGAGGATTCAGAAATGA.............................................................................................................................................................	27	1	1.00	1	0	1	0	0	0	0	0	0	0	0
..........................................................................TCATTACTCGCCCACCGTCTG..................................................................................................................................................................................................................................	21	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.................................................................TCGAAGATCTCATTACTCGCCCACCGTC....................................................................................................................................................................................................................................	28	1	1.00	1	0	1	0	0	0	0	0	0	0	0
..................................................................................................................TAAGCGTCAGCTGCTCAAAACCT........................................................................................................................................................................................	23	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.....................TGACCAAAAA-----------------------ATTTCGTGCAATCGAAGA.........................................................................................................................................................................................................................................................	28	1	1.00	1	0	1	0	0	0	0	0	0	0	0
..........................................................................TCATTACTCGCCCACCGTCTGACAACAAG..........................................................................................................................................................................................................................	29	1	1.00	1	0	1	0	0	0	0	0	0	0	0
..................................................................................................................TAAGCGTCAGCTGCTCAAAACC.........................................................................................................................................................................................	22	1	1.00	1	0	1	0	0	0	0	0	0	0	0
..................................................................................................................................................................................................................................................................................................CTTCGTCGATTTTTCCGCTCATT........	23	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.................................................................................................................................................TCAGAGGATTCAGAAATGAGCCGACT......................................................................................................................................................	26	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.............................................................................................................................................................................................................TCTGAAATTTTGCTTCACTTACGTC...........................................................................................	25	1	1.00	1	0	1	0	0	0	0	0	0	0	0
..................................................................CGAAGATCTCATTACTCGCCCACCGTC....................................................................................................................................................................................................................................	27	1	1.00	1	0	1	0	0	0	0	0	0	0	0
......................................................................................................................................................................................................................TTGCTTCACTTACGTCGCTTAGCACC.................................................................................	26	1	1.00	1	0	1	0	0	0	0	0	0	0	0
..........................................................................TCATTACTCGCCCACCGTCTGA.................................................................................................................................................................................................................................	22	1	1.00	1	0	1	0	0	0	0	0	0	0	0
........................................................................................................................TCAGCTGCTCAAAACCTTCGG....................................................................................................................................................................................	21	1	1.00	1	0	1	0	0	0	0	0	0	0	0
...........................................................................................................................GCTGCTCAAAACCTTCGGAG..................................................................................................................................................................................	20	1	1.00	1	0	1	0	0	0	0	0	0	0	0
................................................................................................................................................................................................................................................TGCT-----------------------------------AATGGAGTTGCCTTCGTCGATT....................	26	1	1.00	1	0	1	0	0	0	0	0	0	0	0
...........................................................................................................................................GGAGAGTCAGAGGATTCAGAAATGAGC...........................................................................................................................................................	27	1	1.00	1	0	1	0	0	0	0	0	0	0	0
..............................................................................TACTCGCCCACCGTCTGACAACAAG..........................................................................................................................................................................................................................	25	3	0.67	2	0	2	0	0	0	0	0	0	0	0
............................................................TGCAATCGAAGATCTCATTACTCGCCC..........................................................................................................................................................................................................................................	27	4	0.50	2	0	1	1	0	0	0	0	0	0	0
............................................................TGCAATCGAAGATCTCATTACTCGCC...........................................................................................................................................................................................................................................	26	4	0.50	2	0	2	0	0	0	0	0	0	0	0
............................................................................................................................................................................................TGACTGGGAAGAAGCCATCTGAA..............................................................................................................	23	3	0.33	1	0	0	0	0	0	0	0	1	0	0
..............................................................................TACTCGCCCACCGTCTGACAACAAGTAC.......................................................................................................................................................................................................................	28	3	0.33	1	0	1	0	0	0	0	0	0	0	0
..............................................................................TACTCGCCCACCGTCTGACAACAAGTA........................................................................................................................................................................................................................	27	3	0.33	1	0	1	0	0	0	0	0	0	0	0
.............................................................................TTACTCGCCCACCGTCTGACAACAAG..........................................................................................................................................................................................................................	26	3	0.33	1	0	0	0	0	0	0	0	1	0	0
...................................................................GAAGATCTCATTACTCGCCC..........................................................................................................................................................................................................................................	20	4	0.25	1	0	1	0	0	0	0	0	0	0	0
.....................................................................................................................................................................................GGAGACATGACTGGGAAG..........................................................................................................................	18	6	0.17	1	0	1	0	0	0	0	0	0	0	0

droWil2

GTGC-ATATAGACGACGA---------------------------------GATCATCATTGCAGTAGAAGATTTGGTTTTACGACCACCTGCGACTGACAAATATCTTGCCATCAAGAATCGTCTGCTTGAGAAATTCGCCGAATCGGCTGAATCAAAATTTCGACGTCTACTTCACGGCGATGGAACATCTGGATCAAAACCATCCGAAATTCTGGCAAACATGCGACGCCTCGCTCCGAATCCGGCAAGTGAAAGCTTAATTCGCACACTCTTTCTTGCAGAGATGCCTCAGTCAATACGTCCAATGCTGACAGTGTG	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
.............................................................................................................................TGCTTGAGAAATTCGCCGAATCGG............................................................................................................................................................................	24	8	1.38	11	0	9	0	2	0
.............................................................................................................................TGCTTGAGAAATTCGCCGAATCGGC...........................................................................................................................................................................	25	8	0.25	2	0	2	0	0	0
.............................................................................................................................TGCTTGAGAAATTCGCCGAATCG.............................................................................................................................................................................	23	8	0.25	2	0	2	0	0	0
...........................................................................................................................................................................................................................AAACATGCGACGCCTCGCT...................................................................................	19	9	0.22	2	0	2	0	0	0
..........................................................................................................................................................TCAAAATTTCGACGTCTACTTCACGGCGA..........................................................................................................................................	29	5	0.20	1	0	1	0	0	0
.......................................................................................................................................................................GTCTACTTCACGGCGATGGAACATCT................................................................................................................................	26	5	0.20	1	0	0	0	1	0
......................................................................................................................................................................................................................CTGGCAAACATGCGACGCCTCGC....................................................................................	23	10	0.20	2	0	2	0	0	0
........................................................................................................................................................................TCTACTTCACGGCGATGGAACATCTGGA.............................................................................................................................	28	5	0.20	1	0	1	0	0	0
........................................................................................................................................................................................................................GGCAAACATGCGACGCCTCG.....................................................................................	20	10	0.20	2	0	2	0	0	0
..........................................................................................................................................................TCAAAATTTCGACGTCTACTTCA................................................................................................................................................	23	5	0.20	1	0	1	0	0	0
.......................................................................................................................................................................GTCTACTTCACGGCGATGGAACA...................................................................................................................................	23	5	0.20	1	0	1	0	0	0
.............................................................................................................................................................................TTCACGGCGATGGAACATCTGGAT............................................................................................................................	24	5	0.20	1	0	1	0	0	0
..........................................................................................................................................................................................AACATCTGGATCAAAACCATCCGA...............................................................................................................	24	5	0.20	1	0	1	0	0	0
................................................................................................................................................................................................................................................................................TCTTTCTTGCAGAGATGCCTCAGTCAA......................	27	11	0.18	2	0	2	0	0	0
................................................................................................................................................................................................................................................................................................GCCTCAGTCAATACGTCCAATG...........	22	12	0.17	2	0	2	0	0	0
.............................................................................................................................TGCTTGAGAAATTCGCCGAATCGGCT..........................................................................................................................................................................	26	8	0.13	1	0	0	0	1	0
............................................................................................................................CTGCTTGAGAAATTCGCCGAATC..............................................................................................................................................................................	23	8	0.13	1	0	1	0	0	0
.............................................................................................................................TGCTTGAGAAATTCGCCGAATC..............................................................................................................................................................................	22	8	0.13	1	0	1	0	0	0
.....................................................................................................................................................................................................................TCTGGCAAACATGCGACGCCTCGCT...................................................................................	25	9	0.11	1	0	1	0	0	0
.......................................................................................................................................................................................................................TGGCAAACATGCGACGCCTCGCT...................................................................................	23	9	0.11	1	0	1	0	0	0
......................................................................................................................................................................................................................CTGGCAAACATGCGACGCCTCGCTC..................................................................................	25	9	0.11	1	0	1	0	0	0
.....................................................................................................................................................................................................................................................CGGCAAGTGAAAGCTTAATTCGCA....................................................	24	10	0.10	1	0	1	0	0	0
............................................................................................................................................................................................................................................................TGAAAGCTTAATTCGCACACTCTTTCT..........................................	27	10	0.10	1	0	1	0	0	0
.................................................................................................................................................................................................................................................................GCTTAATTCGCACACTCTTTCTTGC.......................................	25	10	0.10	1	0	1	0	0	0
.....................................................................................................................GAATCGTCTGCTTGAGAAATTCGC....................................................................................................................................................................................	24	10	0.10	1	0	1	0	0	0
...................................................................................................................................................................................................................................................................TTAATTCGCACACTCTTTCTTGC.......................................	23	10	0.10	1	0	1	0	0	0
...................................................................................................................................................................................................................................................................TTAATTCGCACACTCTTTCTTGCA......................................	24	10	0.10	1	0	1	0	0	0
...............................................................................................................................................................................................................................................................AAGCTTAATTCGCACACTCTT.............................................	21	10	0.10	1	0	1	0	0	0
.......................................................................................................................................................................................................................TGGCAAACATGCGACGCCTCG.....................................................................................	21	10	0.10	1	0	1	0	0	0
......................................................................................................................AATCGTCTGCTTGAGAAATTCG.....................................................................................................................................................................................	22	10	0.10	1	0	0	1	0	0
.....................................................................................................................................................................................................................................................................AATTCGCACACTCTTTCTTGCAGAGA..................................	26	10	0.10	1	0	1	0	0	0
.......................................................................................................................................................................................................................................................................TTCGCACACTCTTTCTTGCAA.....................................	21	10	0.10	1	0	0	0	1	0
..............................................................................................................................................................................................................................................................................ACTCTTTCTTGCAGAGATGCCTCAGTCAA......................	29	11	0.09	1	0	1	0	0	0
..............................................................................................................................................................................................................................................................................ACTCTTTCTTGCAGAGATGCCTC............................	23	11	0.09	1	0	1	0	0	0
................................................................................................................................................................................................................................................................................................GCCTCAGTCAATACGTCCAATGC..........	23	12	0.08	1	0	1	0	0	0
.......................................................................................................................................................................................................................................................................................TGCAGAGATGCCTCAGTCAATACGTCC...............	27	12	0.08	1	0	0	0	1	0
...................................................................................................................................................................................................................................................................................................TCAGTCAATACGTCCAATGCTGACAG....	26	13	0.08	1	0	1	0	0	0
...................................................................................................................................................................................................................................................................................................TCAGTCAATACGTCCAATGCTGACAGT...	27	13	0.08	1	0	1	0	0	0
..............................................................................................................................................................................................................CCGAAATTCTGGCAAACATGCG.............................................................................................	22	14	0.07	1	0	1	0	0	0
....................................................................................................................................................................................................................................................................................TCTTGCAGAGATGCCTCAGTCAA......................	23	17	0.06	1	0	1	0	0	0

droVir3

GGTG---------------------------TCGTTACACTTAAAGAAGGATGTCGCACTCACCATCGAGGATCTCATCACATATCCACCGGTGCATAACAAATACGGCACAT-AGCGAAACGGCTTTTCGAAACATTTGCGGAATCAGAGGACTCAAAATAAAAGAGACTTCTGCAAGGTCGCGACATGACCACCAAGAATCCGTCAGAAATCCTTACACAAATGCGTCGTTTAACACCCGCTAACGGATTCGAAGCCGTTAAGCGCACTATATTTATTGCCGAGCTGGCAATGTCGGTTCGTCCGCTGATTTCTATCTG

Size

Hit Count

Total Norm

Total

M027
Male-body

M028
Head

M047
Female-body

M061
Embryo

SRR060654
Ovary

SRR060655
Testis

SRR060656
Ovary

SRR060657
Testis

SRR060658
Ovary

SRR060659
Testis

SRR060660
Ovary

SRR060661
Embryo

SRR060662
Embryo

SRR060663
Embryo

SRR060664
Male-body

SRR060665
Female-body

SRR060666
Male-body

SRR060667
Female-body

SRR060668
Male-body

SRR060669
Female-body

SRR060670
Testis

SRR060671
Male-body

SRR060672
Female-body

SRR060673
Ovary

SRR060674
Ovary

SRR060675
Ovary

SRR060676
Ovary

SRR060677
Ovary

SRR060678
Testis

SRR060679
Testis

SRR060680
Testis

SRR060681
Testis

SRR060682
Embryo

SRR060683
Testis

SRR060684
Embryo

SRR060685
Embryo

SRR060686
Embryo

SRR060687
Embryo

SRR060688
Ovary

SRR060689
Testis

SRR1106712
embryo_0-2h

SRR1106713
embryo_0-2h

SRR1106714
embryo_2-4h

SRR1106715
embryo_4-6h

SRR1106716
embryo_4-6h

SRR1106717
embryo_6-8h

SRR1106718
embryo_6-8h

SRR1106719
embryo_8-10h

SRR1106720
embryo_8-10h

SRR1106721
embryo_10-12h

SRR1106722
embryo_10-12h

SRR1106723
embryo_12-14h

SRR1106724
embryo_12-14h

SRR1106725
embryo_14-16h

SRR1106726
embryo_14-16h

SRR1106727
larvae

SRR1106728
larvae

SRR1106729
mixed whole adult body

SRR1106730
embryo_16-30h

V047
Embryo

V053
Head

V116
Male-body

GSM1528803
follicle cells

No Reads

droFic1

ACTA-----------------------------------------------AGAGGTTTTGGCTGTGGAAGACTTAGTCATTCGACCACCTGACGAAAACAAGTATA------TAAAAAATCGCTTGTTACAAAAATATGCTCAGTCTCCAGAGTCGAAACTACGCCGACTTTTACAGGGTGGGGGTACATCTGGTTTGAAATTATCTGAAATCTTGAACAACATGCGACGACTTGCTCCGGATTCGGGCAGCGAGAACATTACCCTCACACTTTTCCTGGCAGAGATGCCGGCATCAATTCATCCTACCCTCACCGTCTG	Size	Hit Count	Total Norm	Total
No Reads

droEle1

TAACAATGACGACGACAAATTTGATCACATCACCGTTAACTTGGATCCGGAGGTCGTTTTAGCCGTCGAGGATTTAGTCACGCATCCTCCAGTCGCAAACAAGTTTGACACGCTGAAAGCTCGCTTGCTCGACAAATTTGCAGAGTCAGCAGAATCAAAACTACGGCGTTTACTGCAAGGTGGAGGAACTTCTGGCCTGAAGCCCTCCCAAATTCTAGCTCACATGCGTCGGCTGGCACCGGAATCCAGCAGCGAACCAATAATCCGTACATTGTTTCTTTCTGAAATGCCTCAGAATATTCGTCCGATTTAAACCGTCTG	Size	Hit Count	Total Norm	Total
No Reads

droRho1

CAACAACGATGACAACAAATTTGACTACATCACCGTAAATTTGGATCCAGACGTTGTTGTG--------------------ACACCCGCCAGCGGCTAACAAGTATGATACCCTAAAGTCCCGATTGCTCGATAAGTTTGCAGAGACAGCCGAATCTAAGCTACGCCGTCTACTTCAAGGAGGAGGAACTGCTGGTCTGAAGCCTTCGCAAATTCTGGCTCATATGCGTCGTTTGGCTCCTGAGACAAGCAGTGAGTCAATAATCCGCACACTGTTCCTCGCCGAGATGCCCCAGAACATTCGCCCTATTTTATCGATATG	Size	Hit Count	Total Norm	Total
No Reads

droBia1

CAACAACGATGACGACAAATTTGACTACATCACCGTAAATTTGGATCCAAACGTTGTTTTGACTGTGGAGGATTTAGTCACTCAACCGCCAGCGGCCAACAAGAATGATACTTTAAAGTTTCGGTTGCTCGATAAGTTTGCGGAGTCAGCCGAATCTAAGCTACGCCGCCTACTTCAAGGAGGAGGAACTGCTGGTCTGAAGCCTTCGCAAATTCTGGCTCATATGCGTCGTCTGGCTCCTGAGTCGAGCAGCGAGTCGATAATCCGCACACTGTTCCTTGCCGAGATGCCCCAAAACATTCGCCCTATTTT---------	Size	Hit Count	Total Norm	Total
No Reads

droTak1

CATCGACGACACCGACAAGTTCTACCTTGTGACTCTTCACCTGGAGGAGGGTGTAGTTCTGGCAGTGGAAGACTTAGTCATTCAACCACCCATCGAAAACAAGTACGTAGCAATTAAAAGTCGCTTACTACAAAAATTTGCCGAATCTCCAGAGTCGAAACTACGCCGACTTCTGCAGGGTGGAGGTACATCTGGTTTAAAACCATCTGAGATCCTGGCCAACATGCGTCGACTACCTCCGGACCCGGGCAGCGAGAACATCATTCTCACTCTTTTCCTGGCAGAGATACCGGCGTCGATTCGCCCCACTCTCACTGTGT-	Size	Hit Count	Total Norm	Total
No Reads

droYak3

--------------------------------------------------------TTTGGGCTGTAGAGGATTTGGTCACTCGTCCACCGGCAGACAACAAGTACGCAGCAATCAAAACTCGCTTGCTGCAGAAGTTCGCTGAATCGCCCGAGTCAAAACTGCGGCGACTACTTCAGGGAGGAGGTGCCACCGGATTAAAGCCTTCGGAAATCTTGGCAAATATGCGACGCTTGGCCCCGGATCCTAGCAGCATGAGCATCATTCACACCCTATTCCTCGCTGAGATGCCAGCCTCCATTCGTCCGCTGCTCACCGTATG	Size	Hit Count	Total Norm	Total	M026 Head	M043 Female-body	M056 Embryo	V046 Embryo	V052 Head	V058 Head	V120 Male-body	SRR1275488 Male larvae	SRR1275486 Male prepupae	SRR1275484 Male prepupae	GSM1528802 follicle cells
....................................................................................................................................GAAGTTCGCTGAATCGCCCGAGT......................................................................................................................................................................	23	4	1.25	5	0	0	0	0	0	0	0	0	0	0	5
.................................................................................................................................................................TGCGGCGACTACTTCAGGGAGGAGG.......................................................................................................................................	25	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
...............................................................................................................................................................................................................................................CGGATCCTAGCAGCATGAGCATCA..........................................................	24	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
.........................................................................TTGGTCACTCGTCCACCGGCAGAC................................................................................................................................................................................................................................	24	8	0.50	4	0	4	0	0	0	0	0	0	0	0	0
............................................................................................................................................................................................................TTCGGAAATCTTGGCAAATATGCG.............................................................................................	24	7	0.43	3	0	3	0	0	0	0	0	0	0	0	0
............................................................................................................................................................................................................TTCGGAAATCTTGGCAAATATGCGA............................................................................................	25	7	0.43	3	0	3	0	0	0	0	0	0	0	0	0
............................................................................................................................................................................................................TTCGGAAATCTTGGCAAATATGC..............................................................................................	23	7	0.43	3	0	3	0	0	0	0	0	0	0	0	0
..........................................................................................................................................................................................................................................................................................TGAGATGCCAGCCTCCATTCGTCCGC.............	26	6	0.33	2	0	2	0	0	0	0	0	0	0	0	0
...............................................................................................................................................................................................................................................................................................TGCCAGCCTCCATTCGTCCGCTGC..........	24	6	0.33	2	0	2	0	0	0	0	0	0	0	0	0
.........................................................................TTGGTCACTCGTCCACCGGCAG..................................................................................................................................................................................................................................	22	11	0.27	3	0	3	0	0	0	0	0	0	0	0	0
.......................................................................................................................................GTTCGCTGAATCGCCCGAGTCAAAAC................................................................................................................................................................	26	4	0.25	1	0	1	0	0	0	0	0	0	0	0	0
..........................................................................................................................................................................................................................................................................ACACCCTATTCCTCGCTGAGATGCC..............................	25	4	0.25	1	0	1	0	0	0	0	0	0	0	0	0
........................................................................................................................................TTCGCTGAATCGCCCGAGTCAAAACT...............................................................................................................................................................	26	4	0.25	1	0	1	0	0	0	0	0	0	0	0	0
..................................................................................................................................................................................................................................................................CATCATTCACACCCTATTCCTCG........................................	23	4	0.25	1	0	1	0	0	0	0	0	0	0	0	0
.....................................................................................................................................................................................................................................................................CATTCACACCCTATTCCTCGCTGAGA..................................	26	4	0.25	1	0	1	0	0	0	0	0	0	0	0	0
.................................................................................................................................................................................................................................................................GCATCATTCACACCCTATTCCT..........................................	22	4	0.25	1	0	1	0	0	0	0	0	0	0	0	0
................................................................................................................................TGCAGAAGTTCGCTGAATCGCCCGAGT......................................................................................................................................................................	27	4	0.25	1	0	0	1	0	0	0	0	0	0	0	0
.........................................................................................................................................................................................................................................................................CACACCCTATTCCTCGCTGAGATGCC..............................	26	4	0.25	1	0	1	0	0	0	0	0	0	0	0	0
.................................................................................................................................................................................................................................................................GCATCATTCACACCCTATTCCTCGC.......................................	25	4	0.25	1	0	1	0	0	0	0	0	0	0	0	0
.......................................................................................................TACGCAGCAATCAAAACTCGCTTGC.................................................................................................................................................................................................	25	4	0.25	1	0	1	0	0	0	0	0	0	0	0	0
........................................................................................................................................TTCGCTGAATCGCCCGAGTCAAAAC................................................................................................................................................................	25	4	0.25	1	0	1	0	0	0	0	0	0	0	0	0
.......................................................................................................................................GTTCGCTGAATCGCCCGAGTC.....................................................................................................................................................................	21	4	0.25	1	0	0	1	0	0	0	0	0	0	0	0
............................................................................................................................TTGCTGCAGAAGTTCGCTGAATCGCC...........................................................................................................................................................................	26	4	0.25	1	0	1	0	0	0	0	0	0	0	0	0
.............................................................................................................GCAATCAAAACTCGCTTGCTGCAGA...........................................................................................................................................................................................	25	5	0.20	1	0	1	0	0	0	0	0	0	0	0	0
...............................................................................................................................................................................................................................................................................................TGCCAGCCTCCATTCGTCCGCTGCTT........	26	6	0.17	1	0	1	0	0	0	0	0	0	0	0	0
...............................................................................................................................................................................................................................................................................................TGCCAGCCTCCATTCGTCCGCTGCTC........	26	6	0.17	1	0	1	0	0	0	0	0	0	0	0	0
...........................................................................................................................................................................................................................................................................................GAGATGCCAGCCTCCATTCGTCCGC.............	25	6	0.17	1	0	1	0	0	0	0	0	0	0	0	0
.............................................................................................................................................................................................................TCGGAAATCTTGGCAAATATGCGACGC.........................................................................................	27	7	0.14	1	0	1	0	0	0	0	0	0	0	0	0
............................................................................................................................................................................................................TTCGGAAATCTTGGCAAATATGCGAC...........................................................................................	26	7	0.14	1	0	1	0	0	0	0	0	0	0	0	0
.............................................................................................................................................................................................................TCGGAAATCTTGGCAAATATGCGAC...........................................................................................	25	7	0.14	1	0	1	0	0	0	0	0	0	0	0	0
.............................................................................................................................................................................................................TCGGAAATCTTGGCAAATATGCG.............................................................................................	23	7	0.14	1	0	1	0	0	0	0	0	0	0	0	0
.............................................................................................................................................................................................................TCGGAAATCTTGGCAAATATGCGA............................................................................................	24	7	0.14	1	0	1	0	0	0	0	0	0	0	0	0
............................................................................................................................................................................................................TTCGGAAATCTTGGCAAATATGCGACGC.........................................................................................	28	7	0.14	1	0	1	0	0	0	0	0	0	0	0	0
....................................................................................................................................................................................................................TCTTGGCAAATATGCGACGCTTGGCC...................................................................................	26	8	0.13	1	0	0	1	0	0	0	0	0	0	0	0
.......................................................................................................................................................................................................................................................................................................TCCATTCGTCCGCTGCTCAC......	20	8	0.13	1	0	1	0	0	0	0	0	0	0	0	0
.........................................................................TTGGTCACTCGTCCACCGGCAGACA...............................................................................................................................................................................................................................	25	8	0.13	1	0	1	0	0	0	0	0	0	0	0	0
.......................................................................................................................................................................................................................................................................................................TCCATTCGTCCGCTGCTCACCGT...	23	8	0.13	1	0	1	0	0	0	0	0	0	0	0	0
......................................................................................................................................................................................................................TTGGCAAATATGCGACGCTTGGCC...................................................................................	24	8	0.13	1	0	1	0	0	0	0	0	0	0	0	0
.......................................................................................................................................................................................................................................................................................................TCCATTCGTCCGCTGCTCACC.....	21	8	0.13	1	0	1	0	0	0	0	0	0	0	0	0
.........................................................................TTGGTCACTCGTCCACCGGCAGA.................................................................................................................................................................................................................................	23	11	0.09	1	0	1	0	0	0	0	0	0	0	0	0
............................................................................................................................................................................................................................AATATGCGACGCTTGGCC...................................................................................	18	12	0.08	1	0	1	0	0	0	0	0	0	0	0	0
.........................................................................TTGGTCACTCGTCCACCG......................................................................................................................................................................................................................................	18	12	0.08	1	0	1	0	0	0	0	0	0	0	0	0
.........................................................................TTGGTCACTCGTCCACCGGC....................................................................................................................................................................................................................................	20	12	0.08	1	0	1	0	0	0	0	0	0	0	0	0

droEre2

TGTCGACGACAATGGTGA--------------CACGGCATTTTGA---ATATGTGATTTTGGTTGTGGATGGTTTGGTCACTCGTCCATCGGCAGATAACAAGTAAGCAGCAATCAAAACTCGCTTGCTGCAGAAGGTCGCTGAGTCGCCCGAATCAAAACTGCGACGACTACTTCAAGGAGGAGGTGCCACCGGATTAATGCCTTCGAAAATCTTGGAAAATATGCGACGCTCGGCCCCTGATCCTGGCAGCGAAAGCATCATTCACACCCTATTCCTCGCTGAGATGTCAGCCTCCATTCGTCCGCTGCACACCGTATG	Size	Hit Count	Total Norm	Total	M055 Female-body	M057 Embryo	V060 Head	V040 Embryo	V107 Male-body	V108 Head	GSM1528801 follicle cells
No Reads

-10.9	-10.6	-10.4	-10.4

dpe_832

scaffold_159:54123-54273 -

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads