dpe_688

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------################################################## GCCGCTAATATTAAACGACACGACAAAGAGTGCGTGCGAGAGAGACAGAAAATCAGTCTGAGCGTGACGTCGGGCGCTGCGTAGCCACTGCAAATTGATTTGTTCCTTTTGGCTATAAAAATTATCTGATCTGATCCAGATTCAGCAATTTGATAGATATGGTCATTATCAATGATAATGATAATGATCTGGATGCAACAGATTTTCGTCGTATGTGGGAGCGTTTGCTTTCATCCTCGAGGAGGAAATCG ************************************************.....((((((........))))))..(((((..(((((((((((((.(((((.((....))..)))))......((((((((...)))).))))))))))).)))....(((((((((((.......)))))))))))))))))))....************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M042 female body	V111 male body
.............................................................................................................................................................................................................TCGTCGTATGTGGGAGCGTTT.........................	21	0	1	1.00	1	1	0
..............................................................................................................................................................................................................CGTCGTATGTGGGAACGTT..........................	19	1	1	1.00	1	0	1
..............................TGCGTGCGAGAGAGACAGAAA........................................................................................................................................................................................................	21	0	20	0.55	11	0	11
....................................................................................................................................................................................AAAAGGCTCTGGATGCAACAG..................................................	21	3	3	0.33	1	1	0
........................................................................................................................ATTATCTGATCTGATCCAGAT..............................................................................................................	21	0	20	0.10	2	2	0
............................................................................................................................................TTCAGCAATTTGATAGATATGGT........................................................................................	23	0	12	0.08	1	1	0
............................................................................................................................TCTGATCTGATCCAGATTCAG..........................................................................................................	21	0	20	0.05	1	1	0
............................................................................................................TTGGCTATAAAAATTATCTGATCTGATC...................................................................................................................	28	0	20	0.05	1	1	0
...................................................................................................................TAAAAATTATCTGATCTGATCCAGAT..............................................................................................................	26	0	20	0.05	1	1	0

CGGCGATTATAATTTGCTGTGCTGTTTCTCACGCACGCTCTCTCTGTCTTTTAGTCAGACTCGCACTGCAGCCCGCGACGCATCGGTGACGTTTAACTAAACAAGGAAAACCGATATTTTTAATAGACTAGACTAGGTCTAAGTCGTTAAACTATCTATACCAGTAATAGTTACTATTACTATTACTAGACCTACGTTGTCTAAAAGCAGCATACACCCTCGCAAACGAAAGTAGGAGCTCCTCCTTTAGC ************************************************.....((((((........))))))..(((((..(((((((((((((.(((((.((....))..)))))......((((((((...)))).))))))))))).)))....(((((((((((.......)))))))))))))))))))....************************************************	Read size	Hit Count	Total Norm	Total	M042 female body
............................................................................................................................................................................................................................CGCAAACGAAAGTAGGAGCTCCTCCTT....	27	1	1.00	1	1
..................................ACGCTCTCTCTGTCTTTTAGTCAGACT..............................................................................................................................................................................................	27	20	0.20	4	4
..............................................................................................................CCGATATTTTTAATAGACTAGACT.....................................................................................................................	24	20	0.10	2	2
..............................................................................................................................ACTAGACTAGGTCTAAGTCGTTAA.....................................................................................................	24	20	0.10	2	2
......................................................................................................................................AGGTCTAAGTCGTTAAACTATCTA.............................................................................................	24	10	0.10	1	1
...............................................................................................................................CTAGACTAGGTCTAAGTCGTTAA.....................................................................................................	23	20	0.05	1	1
.................................................................................................................................AGACTAGGTCTAAGTCGTTAA.....................................................................................................	21	20	0.05	1	1
............................................................................................................................AGACTAGACTAGGTCTAAGTCGTTAA.....................................................................................................	26	20	0.05	1	1

Species	Coordinate	ID	Alignment
droPer2	scaffold_1112:2811-3061 +	dpe_688	GCCGCTAATATTAAACGACACGACAAAGAGTGCGTGCGAGAGAGACAGAAAATCAGTCTGAGCGTGACGTCGGGCGCTGCGTAGCCACTGCAAATTGATTTGTTCCTTTTGGCTATAAAAATTATCTGATCTGATCCAGATTCAGCAATTTGATAGATATGGTCATTATCAATGATAATGATAATGATC---------------TGGATGCAACAGATTTTCGTCGTATGTGGGAGCGTTTGCTTTCATCCTCGAGGAGGAAATCG
dp5	Unknown_group_46:7365-7585 -		GCCGCTAATATTGAACGACACGACAAAGAGTGCGTGCGAGAGAGACAGAAAATCAGTCTGAGCGTGACGTCGGGCGCTGCGTAGCCACTGCAAATTGATTTGTTCCTTTTGGCTATAAAAATGATCTGATCTGATCCAGATTCAGCAATCTAATAGATATGGTCATTATC-----------------TCAAATCTGCAATATTGTGGATGCAACAGATTTTCGTCCTTTGTGGGGGCG----------------------------
droWil2	scf2_1100000004909:5953295-5953345 +		A-------------------------------------------------------------------------------------------------------------------------------------TATAGACACAGCTATCTGA----GATGATAATGATTAATGATGATGATAATGAT------------------------------------------------------------------------------
droGri2	scaffold_15203:4079690-4079717 -		G---------------------ACAGAGAGAGCGTGAGAGAGAGACAGA-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droKik1	scf7180000301684:8067-8103 -		-------------------------------------------------------------------------------CGAAGCCCATGCAAATTGTTTTGTTTTTTTTGGACAT------------------------------------------------------------------------------------------------------------------------------------------------------

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droPer2

GCCGCTAATATTAAACGACACGACAAAGAGTGCGTGCGAGAGAGACAGAAAATCAGTCTGAGCGTGACGTCGGGCGCTGCGTAGCCACTGCAAATTGATTTGTTCCTTTTGGCTATAAAAATTATCTGATCTGATCCAGATTCAGCAATTTGATAGATATGGTCATTATCAATGATAATGATAATGATC---------------TGGATGCAACAGATTTTCGTCGTATGTGGGAGCGTTTGCTTTCATCCTCGAGGAGGAAATCG	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
............................................................................................................................................................................................................................TCGTCGTATGTGGGAGCGTTT.........................	21	1	1.00	1	0	1	0	0	0	0
..............................TGCGTGCGAGAGAGACAGAAA.......................................................................................................................................................................................................................	21	20	0.55	11	0	0	0	0	0	11
........................................................................................................................ATTATCTGATCTGATCCAGAT.............................................................................................................................	21	20	0.10	2	0	2	0	0	0	0
............................................................................................................................................TTCAGCAATTTGATAGATATGGT.......................................................................................................	23	12	0.08	1	0	1	0	0	0	0
............................................................................................................................TCTGATCTGATCCAGATTCAG.........................................................................................................................	21	20	0.05	1	0	1	0	0	0	0
............................................................................................................TTGGCTATAAAAATTATCTGATCTGATC..................................................................................................................................	28	20	0.05	1	0	1	0	0	0	0
...................................................................................................................TAAAAATTATCTGATCTGATCCAGAT.............................................................................................................................	26	20	0.05	1	0	1	0	0	0	0

dp5

GCCGCTAATATTGAACGACACGACAAAGAGTGCGTGCGAGAGAGACAGAAAATCAGTCTGAGCGTGACGTCGGGCGCTGCGTAGCCACTGCAAATTGATTTGTTCCTTTTGGCTATAAAAATGATCTGATCTGATCCAGATTCAGCAATCTAATAGATATGGTCATTATC-----------------TCAAATCTGCAATATTGTGGATGCAACAGATTTTCGTCCTTTGTGGGGGCG----------------------------	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
...................................................ATCAGTCTGAGCGTGACGTCGGGC...............................................................................................................................................................................................	24	20	7.00	140	0	0	140	0	0	0	0	0	0	0
..........................................................TGAGCGTGACGTCGGGCGC.............................................................................................................................................................................................	19	20	2.15	43	0	43	0	0	0	0	0	0	0	0
......................................................AGTCTGAGCGTGACGTCGGG................................................................................................................................................................................................	20	20	1.10	22	0	0	0	0	0	0	22	0	0	0
.......................................................................................................................................................................ATC-----------------TCAAATCTGCAATATTGTGGAT.........................................................	25	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.....................................................................................................................................................................TTATC-----------------TCAAATCTGCAATATTGTGT...........................................................	25	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.......................................................GTCTGAGCGTGACGTCGGG................................................................................................................................................................................................	19	20	0.60	12	0	0	0	0	0	0	12	0	0	0
.....................................................................................................................................................................................................................CAGATTTTCGTCCTTTGTGG.................................	20	20	0.60	12	0	12	0	0	0	0	0	0	0	0
.......................................................................................................TCCTTTTGGCTATAAAAATGATCTG..........................................................................................................................................	25	20	0.55	11	0	11	0	0	0	0	0	0	0	0
........................................................................................................................................................................................................................ATTTTCGTCCTTTGTGGGGG..............................	20	20	0.40	8	0	0	0	0	0	0	0	0	8	0
......................................................................................................TTCCTTTTGGCTATAAAAATGATCTGA.........................................................................................................................................	27	20	0.40	8	0	8	0	0	0	0	0	0	0	0
.....................GACAAAGAGTGCGTGCGAGAGAGA.............................................................................................................................................................................................................................	24	20	0.40	8	0	8	0	0	0	0	0	0	0	0
...........................................................................................................................................................................................TCAAATCTGCAATATTGTGGATG........................................................	23	20	0.35	7	0	7	0	0	0	0	0	0	0	0
......................................................................CGGGCGCTGCGTAGCCACTGC...............................................................................................................................................................................	21	20	0.25	5	0	5	0	0	0	0	0	0	0	0
....CTAATATTGAACGACACGAC..................................................................................................................................................................................................................................................	20	20	0.25	5	0	2	0	0	0	0	0	0	3	0
........TATTGAACGACACGACAAAGAGTGC.........................................................................................................................................................................................................................................	25	20	0.25	5	0	0	1	0	0	0	0	4	0	0
...........................................................................................................TTTGGCTATAAAAATGATCTGATCTGA....................................................................................................................................	27	20	0.20	4	0	4	0	0	0	0	0	0	0	0
.....TAATATTGAACGACACGACAA................................................................................................................................................................................................................................................	21	20	0.20	4	0	4	0	0	0	0	0	0	0	0
............................................................AGCGTGACGTCGGGCGCTGCGT........................................................................................................................................................................................	22	20	0.15	3	0	3	0	0	0	0	0	0	0	0
.........ATTGAACGACACGACAAAGAG............................................................................................................................................................................................................................................	21	20	0.15	3	0	3	0	0	0	0	0	0	0	0
...................................................................................................................................TGATCCAGATTCAGCAATCTA..................................................................................................................	21	20	0.15	3	0	3	0	0	0	0	0	0	0	0
......................................AGAGAGACAGAAAATCAGTC................................................................................................................................................................................................................	20	20	0.15	3	0	0	0	0	0	0	3	0	0	0
.............................................................................................................................................................................................AAATCTGCAATATTGTGGAT.........................................................	20	20	0.15	3	0	3	0	0	0	0	0	0	0	0
..........TTGAACGACACGACAAAGAGT...........................................................................................................................................................................................................................................	21	20	0.15	3	0	3	0	0	0	0	0	0	0	0
.........................................GAGACAGAAAATCAGTCTGAGCG..........................................................................................................................................................................................................	23	20	0.15	3	0	0	0	0	0	0	0	3	0	0
................................................................................GTAGCCACTGCAAATTGATTT.....................................................................................................................................................................	21	20	0.15	3	0	3	0	0	0	0	0	0	0	0
..............................................................................................................................................................................................................GATGCAACAGATTTTCGTCC........................................	20	20	0.15	3	0	0	1	0	0	0	2	0	0	0
......................................................................................................................AAATGATCTGATCTGATCCAG...............................................................................................................................	21	20	0.10	2	0	0	0	0	0	0	0	0	2	0
...........................................................................................................TTTGGCTATAAAAATGATC............................................................................................................................................	19	20	0.10	2	0	2	0	0	0	0	0	0	0	0
...................................................................................................................................TGATCCAGATTCAGCAATC....................................................................................................................	19	20	0.10	2	0	2	0	0	0	0	0	0	0	0
....................................................................................................TGTTCCTTTTGGCTATAAA...................................................................................................................................................	19	20	0.10	2	0	2	0	0	0	0	0	0	0	0
.............................................................................TGCGTAGCCACTGCAAATTGATTT.....................................................................................................................................................................	24	20	0.10	2	0	2	0	0	0	0	0	0	0	0
......AATATTGAACGACACGACAAAGAG............................................................................................................................................................................................................................................	24	20	0.10	2	0	2	0	0	0	0	0	0	0	0
......................................................................................................................................TCCAGATTCAGCAATCTAATAGAT............................................................................................................	24	20	0.10	2	0	2	0	0	0	0	0	0	0	0
...................ACGACAAAGAGTGCGTGCGAGAGAGACA...........................................................................................................................................................................................................................	28	20	0.10	2	0	2	0	0	0	0	0	0	0	0
.......................CAAAGAGTGCGTGCGAGAG................................................................................................................................................................................................................................	19	20	0.10	2	0	0	0	0	0	2	0	0	0	0
...............................................................GTGACGTCGGGCGCTGCGTAG......................................................................................................................................................................................	21	20	0.10	2	0	0	0	0	0	0	2	0	0	0
........................................AGAGACAGAAAATCAGTCTG..............................................................................................................................................................................................................	20	20	0.10	2	0	2	0	0	0	0	0	0	0	0
.......................................................................................................................AATGATCTGATCTGATCCAGATTC...........................................................................................................................	24	20	0.10	2	0	2	0	0	0	0	0	0	0	0
........TATTGAACGACACGACAAAG..............................................................................................................................................................................................................................................	20	20	0.10	2	0	2	0	0	0	0	0	0	0	0
.........................................................CTGAGCGTGACGTCGGGCGCTGC..........................................................................................................................................................................................	23	20	0.10	2	0	2	0	0	0	0	0	0	0	0
............GAACGACACGACAAAGAGTGC.........................................................................................................................................................................................................................................	21	20	0.10	2	0	0	0	0	0	0	2	0	0	0
..............ACGACACGACAAAGAGTGCG........................................................................................................................................................................................................................................	20	20	0.10	2	0	2	0	0	0	0	0	0	0	0
.....TAATATTGAACGACACGACAAAG..............................................................................................................................................................................................................................................	23	20	0.10	2	0	0	0	0	0	0	0	2	0	0
.................................................AAATCAGTCTGAGCGTGACGTCGGG................................................................................................................................................................................................	25	20	0.10	2	0	0	0	0	2	0	0	0	0	0
...................................................ATCAGTCTGAGCGTGACGT....................................................................................................................................................................................................	19	20	0.05	1	0	0	0	0	0	0	0	1	0	0
............................................................................................................................TCTGATCTGATCCAGATTCAG.........................................................................................................................	21	20	0.05	1	0	0	0	0	1	0	0	0	0	0
........................................................................................................................................................................TC-----------------TCAAATCTGCAATATTGTGGATGA.......................................................	26	20	0.05	1	0	1	0	0	0	0	0	0	0	0
...................ACGACAAAGAGTGCGTGCGAG..................................................................................................................................................................................................................................	21	20	0.05	1	0	0	0	0	0	0	1	0	0	0
................................................................................................................................ATCTGATCCAGATTCAGCAAT.....................................................................................................................	21	20	0.05	1	0	0	0	0	0	0	1	0	0	0
...........TGAACGACACGACAAAGAGTGCGT.......................................................................................................................................................................................................................................	24	20	0.05	1	0	1	0	0	0	0	0	0	0	0
.........................................................CTGAGCGTGACGTCGG.................................................................................................................................................................................................	16	20	0.05	1	0	0	0	0	0	0	0	0	1	0
........................................................................................................CCTTTTGGCTATAAAAATGATCTGA.........................................................................................................................................	25	20	0.05	1	0	0	0	0	1	0	0	0	0	0
...................................................................................................................................TGATCCAGATTCAGCAATCTAATAGATA...........................................................................................................	28	20	0.05	1	0	0	0	0	0	0	0	0	1	0
.......................CAAAGAGTGCGTGCGAGAGAG..............................................................................................................................................................................................................................	21	20	0.05	1	0	0	0	0	1	0	0	0	0	0
...........................................................................................................TTTGGCTATAAAAATGATCTGATCTG.....................................................................................................................................	26	20	0.05	1	0	0	0	0	0	0	1	0	0	0
......................................................................................ACTGCAAATTGATTTGTTCCTTTTGGCTA.......................................................................................................................................................	29	20	0.05	1	0	1	0	0	0	0	0	0	0	0
...GCTAATATTGAACGACACGAC..................................................................................................................................................................................................................................................	21	20	0.05	1	0	1	0	0	0	0	0	0	0	0
...........................................................................................................................................ATTCAGCAATCTAATAGATAT..........................................................................................................	21	20	0.05	1	0	1	0	0	0	0	0	0	0	0
...............................................................................................................................................AGCAATCTAATAGATATGGTC......................................................................................................	21	20	0.05	1	0	1	0	0	0	0	0	0	0	0
..................................................................................................................................................................................................................CAACAGATTTTCGTCCTTTGTGGGGG..............................	26	20	0.05	1	0	0	0	0	0	0	1	0	0	0
...............................................................................................TGATTTGTTCCTTTTGGCTATA.....................................................................................................................................................	22	20	0.05	1	0	0	0	0	0	1	0	0	0	0
......AATATTGAACGACACGACAAAGAGT...........................................................................................................................................................................................................................................	25	20	0.05	1	0	1	0	0	0	0	0	0	0	0

droWil2

A-------------------------------------------------------------------------------------------------------------------------------------TATAGACACAGCTATCTGA----GATGATAATGATTAATGATGATGATAATGAT------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
No Reads

droGri2

G---------------------ACAGAGAGAGCGTGAGAGAGAGACAGA-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M041 Female-body	V109 Male-body
No Reads

droKik1

-------------------------------------------------------------------------------CGAAGCCCATGCAAATTGTTTTGTTTTTTTTGGACAT------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

dpe_688

scaffold_1112:2861-3011 +

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-37.1	-36.9	-36.9