dpe_318

CTCGACTCGAGTGCCACAAATGCCACATGATGCAAATGGAGCAGTCGTGTTTTAAGGTACAGACTTTGAGTCTATTCTAGGCATCAATTTTCGTATCTTTTAATTCGTCTGCCGCCAACAGCAACAGCTACAAAACGAGTAACGAGGAGAGATAA *********************************.(((.((((.((.(((..(((((((.((..(((((((((...))))).))))..)).)))))))..))).)).))))..)))...*********************************	Read size	# Mismatch	Hit Count	Total Norm	Total	V111 male body	M042 female body	V050 head	V042 embryo
..................................................TTTAAGGTACAGACTTTGAGTC...................................................................................	22	0	1	234.00	234	232	1	1	0
..................................................TTTAAGGTACAGACTTTGAGTCT..................................................................................	23	0	1	37.00	37	37	0	0	0
..................................................TTTAAGGTACAGACTTTGAG.....................................................................................	20	0	1	32.00	32	32	0	0	0
..................................................TTTAAGGTACAGACTTTGAGT....................................................................................	21	0	1	15.00	15	14	1	0	0
..................................................TTTAAGGTACAGACTTTGA......................................................................................	19	0	1	5.00	5	5	0	0	0
..................................................TTTAAGGTACAGACTTTGAGTT...................................................................................	22	1	1	5.00	5	5	0	0	0
..................................................TTTAAGGTACAGACTTTGAGTTT..................................................................................	23	1	1	5.00	5	5	0	0	0
..................................................TTTAAGGTACGGACTTTGAGTC...................................................................................	22	1	1	3.00	3	3	0	0	0
..................................................TTTAAGGTACAGACTTTGGG.....................................................................................	20	1	1	2.00	2	2	0	0	0
..................................................TTTAAGGTACATACTTTGAGTC...................................................................................	22	1	1	1.00	1	1	0	0	0
..................................................TTTAAGGTACAGACTTCGAGTC...................................................................................	22	1	1	1.00	1	1	0	0	0
..................................................TTTAAGGTACAGACGTTGAGTCT..................................................................................	23	1	1	1.00	1	1	0	0	0
..................................................CTTAAGGTACAGACTTTGAGTC...................................................................................	22	1	1	1.00	1	1	0	0	0
...................................................TTAAGGTACAGGCTTTGAGTC...................................................................................	21	1	1	1.00	1	1	0	0	0
..................................................TTTAAGGTACAGACTTTGAGTA...................................................................................	22	1	1	1.00	1	1	0	0	0
..................................................TTTAAGGTAAAGACTTTGAGTC...................................................................................	22	1	1	1.00	1	1	0	0	0
..................................................TTTAAGGTACAGGCTTTGAGTC...................................................................................	22	1	1	1.00	1	1	0	0	0
..................................................TTTAAGGTACAGACTTTGAGAC...................................................................................	22	1	1	1.00	1	1	0	0	0
..................................................TTTAAGGTACAGACTTTGAGTCA..................................................................................	23	1	1	1.00	1	1	0	0	0
..................................................TTTAAGGAACAGACTTTGAG.....................................................................................	20	1	2	0.50	1	1	0	0	0
.................................ACATGGAGCCGTCGTATTT.......................................................................................................	19	3	19	0.05	1	0	0	0	1

Species	Coordinate	ID	Type	Alignment
droPer2	scaffold_10:2201134-2201288 -	dpe_318	candidate-rescued	CTCGACTCGA-------GTGCCACAAATGCCACATGATGC----------AAAT-------------GGAGCAGTCGTGTTTTAAGGTACAGACTTTG---------AG---------TCTATT-CTAGGCATCAATTTTCGTATCTTTTAATTCGTCTGCCGC-CAACAGCAACAGC-TACAAAA---------------------------CGAGTAACGAGGAGAGATAA
dp5	4_group4:3182203-3182357 -	dps-mir-2509	Known_mirbase	CTCGACTCGA-------GTGCCACAAATGCCACATGATGC----------AAAT-------------GGAGCAGTCGTGTTTTAAGGTACAGACTTTG---------AG---------TCTATT-CTAGGCATCAATTTTCGTATCTTTTAATTCGTCTGGCGC-CAACAGCAACAGC-TACAAAA---------------------------CGAGTAACGAGGAGAGATAA
droWil2	scf2_1100000004521:8625841-8626037 -	dwi_150	confident	CCTGGCTTAC-----AAAAGCTATAATTGCT-------GAAATGGATAC-ACGTTTTCTATTTAATAAGGACCCTCAAGTTATAAGGTAGAGAAAATGTTGTCTTTATATTTGTAACAATTGT--TAAGACATCACTTTCACTAGCTTTCAATTTGTCTGCTCCACAT--GCAACAACATATAATACAATCAAACAAAATGATAAACATCA-------------------TAA
droAna3	scaffold_12916:11085079-11085208 +	dan_24	confident	CTCGACTTTG-------GG--------CCC----------AACC--ACCTGGCC--------G----AGGGCACTCAAGCTATAAGGTAGAGAAAATAATGTCTTCGTC---------TCCAA--ATAGACATCAATTTCCCTAGCTTGCATTTTGCCTGCCCC-CAA--GCAACAA-------------------------------TGA-------------------TAA
droBip1	scf7180000396572:742828-742949 +			CACGACTTTG-------GG--------CCC----------AACC--ACCTGGTC--------G----GGGGCACCCAAGCTATAAGGTAGAGAAAGTAATGTCTTCGTC---------TCCAA--ATAGACATCAATTTCCCTAGCTTGCAGTTTGCTTGCCCT-CAA--GCAAC----------------------------------------------------------
droKik1	scf7180000302389:430829-430969 -			CTCGTCTCGGAA-C-------------TCCAACATCCTGGAACT--CCCTGCCC-------------AAGGCACTCAAGCTATAAGGTAGAGAAATGGCTGTCTTG-AC---------TCTAAGCTCGGACATCAATTTCACTAGCTTTCAATTTGTCTGCCCC-CAC--GCAACAG-------------------------------TGA-------------------TAA
droFic1	scf7180000453932:197440-197565 -			CTCGGCTCGG-------AA--------GCC----------ATC----CCCCCAC-------------TGGGCAGTCAAGCTATAAGGTAGAGAAACTCTTGTCTTCCCC---------TACAT--CCAGACATCAGTTTCACTAGCTTTCAATTTGTCTGCCCC-CAC--GCAACAA--------------------------------GA-------------------TAG
droEle1	scf7180000490753:30465-30582 +			CTCGA-------------A--------CCC----------AATT--ACCTC----------------GGGGCACCCAAGCTATAAGGTAGAGAAAATGTTGTCTTCC-C---------ACTAC--TCAGACATCAATTTCACTAGCTTTCAATTTGTCTGCCC--CAC--GCAACAG-------------------------------TGA-------------------TAA
droRho1	scf7180000778253:33734-33860 -			CTCGGCTTTG-------AG--------TTC----------AACT--ACCTCCAC-------------GGGGCACTCAAGCTATAAGGTAGAGAAACTGTTGTCTTCCAT---------ACTAC---TGGACATCACTTTCACTAGCTTTCAGCTTGTTTGCCC--CAC--GCAACAG-------------------------------TGA-------------------TAA
droBia1	scf7180000302422:3830824-3830951 +			CTCGACCCAA-------AG--------GCC----------AACC--AACCCGTC--------------GGGCACTCAAGCTATAAGGTAGAGAAATTGTTGTCCCACAC---------TCTAT--TCAGACATCAATTTCACTAGCTTTCAATTTGACTGCCCC-CAT--GCATCAG-------------------------------TGA-------------------TAA
droTak1	scf7180000415871:385561-385689 +			CTCGACTTAA-------AA--------CCC----------AACC--AACTCGAC-------------GGGGCACCCAAGCTATAAGGTAGAGAAACTGTTGTCTGGCAC---------TCAAT--TCAGACATCAATTTCACTAGCTTTCAATTTGTCTGCCCC-CAC--GCAACAG-------------------------------TGA-------------------TAA
droEug1	scf7180000409063:317582-317729 +			CTCGACTTCAAAACCCAAA--------CCC----------AACT--TCCTCGCC-------------GGGGCACACAAGCTCTAAGGTAGAGAAAATGTTGTCTTTGAC---------GCGTT--CTAGACATCAATTTCCCTAGCTTTCAATTTGTCTGCCCC-CAC--GCAACCG-------------------------------GGATACCGATAACGTGG-------A
dm3	chr2L:5641275-5641405 +	dme-mir-962	Known_mirbase	CTCGACTTGA--------A--------TTC----------AACT--ACTTCGAT-------------GGGGCACTCAGGCTATAAGGTAGAGAAATTGATGCTGTCTAC---------ACTAT--TCAGACTTCAGTTTCATTACCTTTCAATTTGTTTGCCCC-CAT--GCAACAG-------------------------------TGATA--------G--------TAG
droSim2	2l:5446022-5446156 +	dsi-mir-962	Known_mirbase	CTCGACTCGA---CTTGAATCTA------C----------AACT--ACCTCGAT-------------GGGGCGCTCAGGCTATAAGGTAGAGAAATTGATGTTGTCTAC---------ACTAT--TCAGACTTCAGTTTCATTGCCTTTCAATTTGTTTGCCCC-CCT--GCAACAG-------------------------------TGA-------------------TAG
droSec2	scaffold_5:3719010-3719144 +	dse_179	candidate-rescued	CTCGACTCGA---CTTGAATCTA------C----------AACT--ACCTCGAT-------------GGGGCATTCAGGCTATAAGGTAGAGAAATTGATGTTGTCTAC---------ACTAT--TCAGACTTCAGTTTCATTGCCTTTCAATTTGTTTGCCCC-CAT--GCAACAG-------------------------------TGG-------------------TAG
droYak3	2L:13551913-13552045 -	dya_92	candidate-rescued	CTCGACTCGA---CTCGAA--------TCC----------AACC--AACTTCAT-------------GGGGCACACAAGCTATAAGGTAGAGAAATTGTTGTCTTCCAC---------ACTAT--TCAGACATCAGTTTAACTATCTTTCAATTTGTTTGCCCC-CAC--GCAACAG-------------------------------TGA-------------------TAA
droEre2	scaffold_4929:5729736-5729863 +	der_24	candidate-rescued	CTCGGCTCGA-------AT--------C-C----------CAGT--ACCTCCTT-------------GGGGCACTCAAGCTATAAGGTAGAAAAATTGTTGTCTTCCAC---------ACTAT--TCGGACTGCAGTTTAACTATCTTTCAATTTGTTTGCCCC-CAC--GCAACAG-------------------------------TGA-------------------CAA

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droPer2

CTCGACTCGA-------GTGCCACAAATGCCACATGATGC----------AAAT-------------GGAGCAGTCGTGTTTTAAGGTACAGACTTTG---------AG---------TCTATT-CTAGGCATCAATTTTCGTATCTTTTAATTCGTCTGCCGC-CAACAGCAACAGC-TACAAAA---------------------------CGAGTAACGAGGAGAGATAA	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
................................................................................TTTAAGGTACAGACTTTG---------AG---------TC.................................................................................................................	22	1	234.00	234	0	1	0	1	0	232
................................................................................TTTAAGGTACAGACTTTG---------AG---------TCT................................................................................................................	23	1	37.00	37	0	0	0	0	0	37
................................................................................TTTAAGGTACAGACTTTG---------AG---------...................................................................................................................	20	1	32.00	32	0	0	0	0	0	32
................................................................................TTTAAGGTACAGACTTTG---------AG---------T..................................................................................................................	21	1	15.00	15	0	1	0	0	0	14
................................................................................TTTAAGGTACAGACTTTG---------A.............................................................................................................................	19	1	5.00	5	0	0	0	0	0	5
................................................................................TTTAAGGTACAGACTTTG---------AG---------TT.................................................................................................................	22	1	5.00	5	0	0	0	0	0	5
................................................................................TTTAAGGTACAGACTTTG---------AG---------TA.................................................................................................................	22	1	1.00	1	0	0	0	0	0	1
................................................................................TTTAAGGTACAGACTTTG---------AG---------TCA................................................................................................................	23	1	1.00	1	0	0	0	0	0	1

dp5

CTCGACTCGA-------GTGCCACAAATGCCACATGATGC----------AAAT-------------GGAGCAGTCGTGTTTTAAGGTACAGACTTTG---------AG---------TCTATT-CTAGGCATCAATTTTCGTATCTTTTAATTCGTCTGGCGC-CAACAGCAACAGC-TACAAAA---------------------------CGAGTAACGAGGAGAGATAA	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
................................................................................TTTAAGGTACAGACTTTG---------AG---------TC.................................................................................................................	22	1	720.00	720	8	6	1	10	0	8	201	0	486	0
................................................................................TTTAAGGTACAGACTTTG---------AG---------...................................................................................................................	20	1	381.00	381	4	3	0	0	0	2	26	0	346	0
................................................................................TTTAAGGTACAGACTTTG---------AG---------T..................................................................................................................	21	1	292.00	292	10	1	0	0	0	1	1	0	278	1
................................................................................TTTAAGGTACAGACTTTG---------AG---------TCT................................................................................................................	23	1	125.00	125	15	8	0	0	0	2	8	0	92	0
...................................................................................................................................ATCAATTTTCGTATCTTTTAAT................................................................................	22	1	25.00	25	0	0	0	0	0	0	0	0	25	0
................................................................................TTTAAGGTACAGACTTTG---------A.............................................................................................................................	19	1	23.00	23	0	1	0	0	0	1	0	0	21	0
................................................................................TTTAAGGTACAGACTTTG---------AG---------TT.................................................................................................................	22	1	15.00	15	0	1	0	0	0	0	4	0	10	0
...................................................................................................................................ATCAATTTTCGTATCTTTTAA.................................................................................	21	1	13.00	13	0	3	0	0	0	0	0	0	10	0
..................................................................................................................................CATCAATTTTCGTATCTTTTAAT................................................................................	23	1	9.00	9	0	0	0	1	0	0	0	0	8	0
................................................................................TTTAAGGTACAGACTTTG---------AG---------TCA................................................................................................................	23	1	7.00	7	0	0	0	0	0	0	0	0	7	0
.............................................................................TGTTTTAAGGTACAGACTTTG---------A.............................................................................................................................	22	1	6.00	6	0	0	0	0	0	0	0	0	6	0
.................................................................................TTAAGGTACAGACTTTG---------AG---------TC.................................................................................................................	21	1	5.00	5	0	0	0	0	0	0	3	0	2	0
................................................................................TTTAAGGTACAGACTTTG---------AG---------A..................................................................................................................	21	1	4.00	4	0	0	0	0	0	0	1	0	3	0
.............................................................................TGTTTTAAGGTACAGACTTTG---------..............................................................................................................................	21	1	2.00	2	0	0	0	0	0	0	0	0	2	0
.................................................................................TTAAGGTACAGACTTTG---------AG---------...................................................................................................................	19	1	2.00	2	0	0	0	0	0	0	0	0	2	0
................................................................................TTTAAGGTACAGACTTTG---------AA---------...................................................................................................................	20	1	2.00	2	0	0	0	0	0	0	0	0	2	0
................................................................................TTTAAGGTACAGACTTTG---------AG---------TA.................................................................................................................	22	1	2.00	2	0	0	0	0	0	0	0	0	2	0
...................................................................................AAGGTACAGACTTTG---------AG---------TCA................................................................................................................	20	1	2.00	2	0	0	0	0	0	0	0	0	2	0
...................................................................................AAGGTACAGACTTTG---------AG---------TC.................................................................................................................	19	1	2.00	2	0	0	0	0	0	0	0	0	2	0
................................................................................TTTAAGGTACAGACTTTG---------AG---------TCCTA..............................................................................................................	25	1	1.00	1	0	0	0	0	0	0	0	0	1	0
................................................................................TTTAAGGTACAGACTTTA---------..............................................................................................................................	18	1	1.00	1	0	0	0	0	0	0	0	0	1	0
.................................................................................TTAAGGTACAGACTTTG---------AG---------T..................................................................................................................	20	1	1.00	1	0	0	0	0	0	0	0	0	1	0
................................................................................TTTAAGGTACAGACTTTG---------..............................................................................................................................	18	1	1.00	1	0	0	0	0	0	0	0	0	1	0
................................................................................TTTAAGGTACAGACTTTG---------AG---------TCC................................................................................................................	23	1	1.00	1	0	0	0	0	0	0	0	0	1	0
................................................................................TTTAAGGTACAGACTTTG---------AG---------TCTA...............................................................................................................	24	1	1.00	1	0	0	0	0	0	0	0	0	1	0
................................................................................TTTAAGGTACAGACTTTG---------AG---------TCTATT-C...........................................................................................................	27	1	1.00	1	0	0	0	0	0	0	0	0	1	0
...................................................................................................................................ATCAATTTTCGTATCTTTTA..................................................................................	20	1	1.00	1	0	0	0	0	0	0	0	0	1	0
.................................................................................TTAAGGTACAGACTTTG---------AG---------TCT................................................................................................................	22	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.....................................................................AGCAGTCGTGTTTTAAGGTAC...............................................................................................................................................	21	1	1.00	1	1	0	0	0	0	0	0	0	0	0
..................................................................................................................................CATCAATTTTCGTATCTTTTAA.................................................................................	22	1	1.00	1	0	0	0	0	0	0	0	0	1	0

droWil2

CCTGGCTTAC-----AAAAGCTATAATTGCT-------GAAATGGATAC-ACGTTTTCTATTTAATAAGGACCCTCAAGTTATAAGGTAGAGAAAATGTTGTCTTTATATTTGTAACAATTGT--TAAGACATCACTTTCACTAGCTTTCAATTTGTCTGCTCCACAT--GCAACAACATATAATACAATCAAACAAAATGATAAACATCA-------------------TAA	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
.................................................................................ATAAGGTAGAGAAAATGTTGTCT.................................................................................................................................	23	1	1876.00	1876	26	11	1762	14	63
.................................................................................ATAAGGTAGAGAAAATGTTGTC..................................................................................................................................	22	1	1235.00	1235	12	4	1179	13	27
.................................................................................ATAAGGTAGAGAAAATGTTGTCC.................................................................................................................................	23	1	53.00	53	7	1	43	0	2
..................................................................................TAAGGTAGAGAAAATGTTGTCT.................................................................................................................................	22	1	32.00	32	0	1	28	1	2
.................................................................................ATAAGGTAGAGAAAATGTTGT...................................................................................................................................	21	6	26.67	160	0	1	156	0	3
.................................................................................ATAAGGTAGAGAAAATGTTGTCA.................................................................................................................................	23	1	23.00	23	0	1	22	0	0
..........................................................................................................................T--TAAGACATCACTTTCACTAGC.......................................................................................	22	1	11.00	11	1	2	4	1	3
.................................................................................ATAAGGTAGAGAAAATGTTG....................................................................................................................................	20	6	6.50	39	0	0	39	0	0
.................................................................................ATAAGGTAGAGAAAATGTTGTCTT................................................................................................................................	24	1	5.00	5	0	0	5	0	0
..........................................................................................................................T--TAAGACATCACTTTCACTAG........................................................................................	21	1	5.00	5	1	1	3	0	0
.................................................................................ATAAGGTAGAGAAAATGTTGTCTA................................................................................................................................	24	1	4.00	4	0	0	3	0	1
.................................................................................ATAAGGTAGAGAAAATGTTGTCTC................................................................................................................................	24	1	3.00	3	0	0	2	0	1
................................................................................TATAAGGTAGAGAAAATGTTGTCT.................................................................................................................................	24	1	3.00	3	0	0	2	0	1
..................................................................................................................................CATCACTTTCACTAGCTTTCA..................................................................................	21	1	3.00	3	0	0	2	0	1
................................................................................TATAAGGTAGAGAAAATGTTGTC..................................................................................................................................	23	1	3.00	3	0	0	3	0	0
.................................................................................ATAAGGTAGAGAAAATGTTGTCG.................................................................................................................................	23	1	3.00	3	1	0	2	0	0
.................................................................................ATAAGGTAGAGAAAATGTTGTCCC................................................................................................................................	24	1	2.00	2	0	0	2	0	0
..................................................................................................................................CATCACTTTCACTAGCTTTCAA.................................................................................	22	1	2.00	2	0	0	2	0	0
..........................................................................................................................T--TAAGACATCACTTTCACTA.........................................................................................	20	1	1.00	1	0	0	0	0	1
..........................................................................................................................T--TAAGACATCACTTTCACTAGCA......................................................................................	23	1	1.00	1	0	0	1	0	0
..................................................................................................................................CATCACTTTCACTAGCTTTC...................................................................................	20	1	1.00	1	0	0	1	0	0
..............................................................TAATAAGGACCCTCAAGTT........................................................................................................................................................	19	1	1.00	1	1	0	0	0	0
.............................................................................................................................TAAGACATCACTTTCACTAGCT......................................................................................	22	1	1.00	1	0	0	0	0	1
..........................................................................................................................T--TAAGACATCACTTTCACTAGCG......................................................................................	23	1	1.00	1	0	0	1	0	0
..........................................................................................................................T--TAAGACATCACTTTCACTAGCT......................................................................................	23	1	1.00	1	1	0	0	0	0
...................................................................................AAGGTAGAGAAAATGTTGTC..................................................................................................................................	20	1	1.00	1	0	0	1	0	0
.................................................................................ATAAGGTAGAGAAAATGTTGTT..................................................................................................................................	22	6	0.83	5	0	0	5	0	0
.................................................................................ATAAGGTAGAGAAAATGTT.....................................................................................................................................	19	6	0.67	4	0	0	4	0	0
.................................................................................ATAAGGTAGAGAAAATGTTGTA..................................................................................................................................	22	6	0.33	2	0	0	2	0	0
.................................................................................ATAAGGTAGAGAAAATGT......................................................................................................................................	18	6	0.17	1	0	0	0	0	1
................................................................................TATAAGGTAGAGAAAATGTTGT...................................................................................................................................	22	6	0.17	1	0	0	1	0	0
..................................................................................TAAGGTAGAGAAAATGTTGT...................................................................................................................................	20	6	0.17	1	0	1	0	0	0
.................................................................................ATAAGGTAGAGAAAATGTCT....................................................................................................................................	20	10	0.10	1	0	0	1	0	0

droAna3

CTCGACTTTG-------GG--------CCC----------AACC--ACCTGGCC--------G----AGGGCACTCAAGCTATAAGGTAGAGAAAATAATGTCTTCGTC---------TCCAA--ATAGACATCAATTTCCCTAGCTTGCATTTTGCCTGCCCC-CAA--GCAACAA-------------------------------TGA-------------------TAA	Size	Hit Count	Total Norm	Total	M044 Female-body	M058 Embryo	V039 Embryo	V055 Head	V105 Male-body	V106 Head
.................................................................................ATAAGGTAGAGAAAATAATGTCT.................................................................................................................................	23	1	1274.00	1274	8	0	0	26	1118	122
.................................................................................ATAAGGTAGAGAAAATAATGTC..................................................................................................................................	22	1	1238.00	1238	26	1	0	57	1110	44
.................................................................................ATAAGGTAGAGAAAATAATGT...................................................................................................................................	21	1	170.00	170	4	0	0	8	152	6
.................................................................................ATAAGGTAGAGAAAATAATG....................................................................................................................................	20	1	79.00	79	4	0	0	3	68	4
..................................................................................................................................CATCAATTTCCCTAGCTTGCAT.................................................................................	22	1	11.00	11	0	0	0	0	8	3
.................................................................................ATAAGGTAGAGAAAATAAT.....................................................................................................................................	19	1	6.00	6	0	0	0	0	4	2
..................................................................................TAAGGTAGAGAAAATAATGTC..................................................................................................................................	21	1	4.00	4	4	0	0	0	0	0
.................................................................................ATAAGGTAGAGAAAATAATGTCC.................................................................................................................................	23	1	3.00	3	0	1	0	0	2	0
.................................................................................ATAAGGTAGAGAAAATAATGTCA.................................................................................................................................	23	1	3.00	3	0	0	0	1	1	1
.................................................................................ATAAGGTAGAGAAAATAATGTT..................................................................................................................................	22	1	3.00	3	0	0	0	0	3	0
................................................................................TATAAGGTAGAGAAAATAATGT...................................................................................................................................	22	1	2.00	2	0	0	0	0	2	0
..................................................................................TAAGGTAGAGAAAATAATGTCT.................................................................................................................................	22	1	2.00	2	1	0	0	0	1	0
.................................................................................ATAAGGTAGAGAAAATAATGTCTTTT..............................................................................................................................	26	1	2.00	2	1	0	0	0	1	0
.................................................................................ATAAGGTAGAGAAAATAATGA...................................................................................................................................	21	1	2.00	2	0	0	0	0	2	0
.................................................................................ATAAGGTAGAGAAAATAATGTCTT................................................................................................................................	24	1	1.00	1	0	0	0	0	1	0
.................................................................................ATAAGGTAGAGAAAATAATGTAA.................................................................................................................................	23	1	1.00	1	0	0	0	0	1	0
..................................................................................................................................CATCAATTTCCCTAGCTTGCATT................................................................................	23	1	1.00	1	0	0	0	1	0	0
.................................................................................ATAAGGTAGAGAAAATAATGTCTTT...............................................................................................................................	25	1	1.00	1	0	0	0	0	1	0
..................................................................................................................................CATCAATTTCCCTAGCTTGCA..................................................................................	21	1	1.00	1	0	0	0	0	1	0
...................................................................................................................................ATCAATTTCCCTAGCTTGCAT.................................................................................	21	1	1.00	1	0	0	0	0	1	0
.................................................................................................................................ACATCAATTTCCCTAGCTTGCAT.................................................................................	23	1	1.00	1	0	0	0	0	1	0
.................................................................................ATAAGGTAGAGAAAATAATGG...................................................................................................................................	21	1	1.00	1	0	0	0	0	1	0
.................................................................................................................................ACATCAATTTCCCTAGCTTGCA..................................................................................	22	1	1.00	1	1	0	0	0	0	0
.................................................................................ATAAGGTAGAGAAAATA.......................................................................................................................................	17	1	1.00	1	0	0	0	1	0	0
.................................................................................ATAAGGTAGAGAAAATAATGC...................................................................................................................................	21	1	1.00	1	0	0	0	0	1	0
...................................................................................AAGGTAGAGAAAATAATGTC..................................................................................................................................	20	1	1.00	1	0	0	0	0	1	0
..................................................................................TAAGGTAGAGAAAATAATGT...................................................................................................................................	20	1	1.00	1	1	0	0	0	0	0
..................................................................................................................................CATCAATTTCCCTAGCTTGC...................................................................................	20	1	1.00	1	0	0	0	1	0	0

droBip1

CACGACTTTG-------GG--------CCC----------AACC--ACCTGGTC--------G----GGGGCACCCAAGCTATAAGGTAGAGAAAGTAATGTCTTCGTC---------TCCAA--ATAGACATCAATTTCCCTAGCTTGCAGTTTGCTTGCCCT-CAA--GCAAC----------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droKik1

CTCGTCTCGGAA-C-------------TCCAACATCCTGGAACT--CCCTGCCC-------------AAGGCACTCAAGCTATAAGGTAGAGAAATGGCTGTCTTG-AC---------TCTAAGCTCGGACATCAATTTCACTAGCTTTCAATTTGTCTGCCCC-CAC--GCAACAG-------------------------------TGA-------------------TAA	Size	Hit Count	Total Norm	Total
No Reads

droFic1

CTCGGCTCGG-------AA--------GCC----------ATC----CCCCCAC-------------TGGGCAGTCAAGCTATAAGGTAGAGAAACTCTTGTCTTCCCC---------TACAT--CCAGACATCAGTTTCACTAGCTTTCAATTTGTCTGCCCC-CAC--GCAACAA--------------------------------GA-------------------TAG	Size	Hit Count	Total Norm	Total
No Reads

droEle1

CTCGA-------------A--------CCC----------AATT--ACCTC----------------GGGGCACCCAAGCTATAAGGTAGAGAAAATGTTGTCTTCC-C---------ACTAC--TCAGACATCAATTTCACTAGCTTTCAATTTGTCTGCCC--CAC--GCAACAG-------------------------------TGA-------------------TAA	Size	Hit Count	Total Norm	Total
No Reads

droRho1

CTCGGCTTTG-------AG--------TTC----------AACT--ACCTCCAC-------------GGGGCACTCAAGCTATAAGGTAGAGAAACTGTTGTCTTCCAT---------ACTAC---TGGACATCACTTTCACTAGCTTTCAGCTTGTTTGCCC--CAC--GCAACAG-------------------------------TGA-------------------TAA	Size	Hit Count	Total Norm	Total
No Reads

droBia1

CTCGACCCAA-------AG--------GCC----------AACC--AACCCGTC--------------GGGCACTCAAGCTATAAGGTAGAGAAATTGTTGTCCCACAC---------TCTAT--TCAGACATCAATTTCACTAGCTTTCAATTTGACTGCCCC-CAT--GCATCAG-------------------------------TGA-------------------TAA	Size	Hit Count	Total Norm	Total
No Reads

droTak1

CTCGACTTAA-------AA--------CCC----------AACC--AACTCGAC-------------GGGGCACCCAAGCTATAAGGTAGAGAAACTGTTGTCTGGCAC---------TCAAT--TCAGACATCAATTTCACTAGCTTTCAATTTGTCTGCCCC-CAC--GCAACAG-------------------------------TGA-------------------TAA	Size	Hit Count	Total Norm	Total
No Reads

droEug1

CTCGACTTCAAAACCCAAA--------CCC----------AACT--TCCTCGCC-------------GGGGCACACAAGCTCTAAGGTAGAGAAAATGTTGTCTTTGAC---------GCGTT--CTAGACATCAATTTCCCTAGCTTTCAATTTGTCTGCCCC-CAC--GCAACCG-------------------------------GGATACCGATAACGTGG-------A	Size	Hit Count	Total Norm	Total
No Reads

dm3

CTCGACTTGA--------A--------TTC----------AACT--ACTTCGAT-------------GGGGCACTCAGGCTATAAGGTAGAGAAATTGATGCTGTCTAC---------ACTAT--TCAGACTTCAGTTTCATTACCTTTCAATTTGTTTGCCCC-CAT--GCAACAG-------------------------------TGATA--------G--------TAG	Size	Hit Count	Total Norm	Total	GSM154618 Ovary	GSM154620 Ovary	GSM154621 Ovary	GSM154622 Ovary	GSM180328 Head	GSM180329 Mixed-body	GSM180330 Embryo	GSM180331 Embryo	GSM180332 Embryo	GSM180333 Embryo	GSM180334 Larvae	GSM180335 Disc	GSM180336 Pupae	GSM180337 S2	GSM239041 Head	GSM239050 Head	GSM239051 S2	GSM239052 S2	GSM246084 Head	GSM266765 S2	GSM280082 Ovary	GSM280083 Ovary	GSM280084 Ovary	GSM280085 Testis	GSM280086 Ovary	GSM280087 S2-NP	GSM280088 S2-NP	GSM312995 Head	GSM313160 Head	GSM313161 Head	GSM313162 Head	GSM313163 Head	GSM313164 Head	GSM313165 Head	GSM343287 Embryo	GSM379050 Ovary	GSM379051 Ovary	GSM379052 Ovary	GSM379053 Ovary	GSM379054 Ovary	GSM379055 Ovary	GSM379056 Ovary	GSM379057 Ovary	GSM379058 Ovary	GSM379059 Ovary	GSM379060 Ovary	GSM379061 Ovary	GSM379062 Ovary	GSM379063 Ovary	GSM379064 Ovary	GSM379065 Ovary	GSM379066 Ovary	GSM379067 Ovary	GSM424739 S2	GSM424740 S2	GSM424741 S2	GSM467729 Female-body	GSM467730 Female-body	GSM467731 Female-body	M002 CS	M003 Male-body	M004 Male-body	M005 ML-DmD32	M006 ML-DmD32	M029 OSC	M030 OSC	M031 OSC	M032 S2-R+	M033 S2-R+	M034 S2-R+	M035 S2-R+	M036 S2-R+	M037 S2-R+	M038 S2-R+	M039 S2-R+	M048 S2-R+	OSS2 OSS	OSS6 OSS	OSS7 OSS	OSS8 OSS	S1 Head	S10 Embryo	S11 Embryo	S12 Embryo	S13 Embryo	S14 Embryo	S15 S2-GMR	S16 Kc	S17 Larvae	S18 Larvae	S19 Pupae	S2 Head	S20 Pupae	S21 Disc	S22 Head	S23 Female-body	S24 Male-body	S25 Larvae	S26 Larvae	S27 Pupae	S28 Pupae	S29 Head	S3 Male-body	S30 S2-GMR	S31 S2	S32 S2	S33 Kc	S34 Kc	S35 Kc	S4 Female-body	S5 Disc	S6 Embryo	S7 Embryo	S8 S2	S9 Embryo	SRR001337 Head	SRR001338 Head	SRR001339 Head	SRR001340 Head	SRR001341 Head	SRR001342 Head	SRR001343 Head	SRR001344 Head	SRR001345 Head	SRR001346 Head	SRR001347 Head	SRR001348 Head	SRR001349 Head	SRR001664 Head	SRR010951 Ovary	SRR010952 Ovary	SRR010953 Ovary	SRR010954 Ovary	SRR010955 Ovary	SRR010956 Ovary	SRR010957 Ovary	SRR010958 Ovary	SRR010959 Ovary	SRR010960 Ovary	SRR014268 Embryo	SRR014269 Embryo	SRR014270 Embryo	SRR014271 Embryo	SRR014272 Embryo	SRR014273 Ovary	SRR014274 Ovary	SRR014275 Ovary	SRR014276 Ovary	SRR014277 Ovary	SRR014278 Ovary	SRR014279 Ovary	SRR014280 Ovary	SRR014281 Ovary	SRR014282 Ovary	SRR023197 S2	SRR023399 S2	SRR023400 S2	SRR023402 S2	SRR023407 S2	SRR029028 S2	SRR029029 S2	SRR029030 S2	SRR029031 S2	SRR029032 S2	SRR029033 S2	SRR029608 Head	SRR029633 Head	SRR030145 gb3	SRR031692 Head	SRR031693 Head	SRR031694 Head	SRR031696 Head	SRR031697 Head	SRR031698 Head	SRR031699 Head	SRR031700 Head	SRR031701 Head	SRR031702 Head	SRR031703 Head	SRR031704 Head	SRR032092 S2	SRR032093 S2	SRR032094 S2	SRR032095 S2-NP	SRR032096 S2-NP	SRR032152 Head	SRR060643 Testis	SRR060644 Ovary	SRR060645 Testis	SRR060646 Ovary	SRR060647 Ovary	SRR060648 Ovary	SRR060649 Ovary	SRR060650 Testis	SRR060651 Ovary	SRR060652 Testis	SRR060653 Ovary	SRR065151 Ovary	SRR065152 Ovary	SRR065153 Ovary	SRR065154 Ovary	SRR065155 Ovary	SRR065800 Ovary	SRR065801 Ovary	SRR065802 Ovary	SRR065803 Ovary	SRR065804 Ovary	SRR065805 Ovary	SRR065806 Ovary	SRR065807 Ovary	SRR097865 S2-NP	SRR097866 S2-NP	SRR097867 S2-NP	SRR298536 Ovary	SRR298537 Ovary	SRR298711 Ovary	SRR298712 Ovary	SRR317110 Ovary	SRR317111 Ovary	SRR317112 Ovary	SRR317113 Ovary	SRR317114 Ovary	SRR317115 Ovary	SRR317116 Ovary	SRR317117 Ovary	SRR341115 S2	SRR341116 S2	SRR341117 S2	SRR341118 S2	SRR351332 Mixed-body	SRR351333 Mixed-body	T004 OSC	T005 CME-L1	V001 Embryo	V002 Embryo	V003 S2	V006 Female-body	V007 Head	V008 S2	V009 CME-L1	V010 ML-DmD20-C5	V011 Sg4	V012 Male-body	V013 Head	V014 DTT	V015 DreRFHV	V018 Kc167	V019 GM2	V020 S2-R+	V021 ML-DmD21	V022 ML-DmD32	V023 Head	V024 Kc167	V025 S2-R+	V026 1182-2H	V027 ML-DmD21	V028 CME-W1-Cl.8+	V029 ML-DmD9	V030 ML-DmD8	V031 GM2	V032 S1	V033 CME-W1-Cl.8+	V034 ML-DmD16-C3	V035 ML-DmD32	V036 ML-DmD20-C5	V037 S2	V038 S2	V063 Ovary	V0632 Ovary	V064 Ovary	V0642 Ovary	V065 Ovary	V0652 Ovary	V066 Ovary	V0662 Ovary	V067 S2-NP	V073 mbn2	V074 S3	V075 ML-DmBG1-C1	V076 ML-DmBG3-C2	V077 Head	V078 Head	V079 Head	V080 Head	V081 Embryo	V082 Embryo	V083 Male-body	V084 Female-body	V085 CME-W2	V086 Female-body	V088 Ovary	V0882 Ovary	V089 Ovary	V0892 Ovary	V090 Male-body	V091 fGS/OSS	V092 Male-body	V093 Male-body	V094 S2-R+	V095 S2-R+	V096 Ovary	V097 Ovary	V098 Ovary	V125 ML-DmD9	V126 CME-L1	V127 G2	V128 S3	V129 ML-DmBG1-C1	V130 ML-DmBG3-C2	V131 ML-DmD16-C3	V132 ML-DmD32	V133 Sg4	V134 ML-DmD8	V135 CME-W2	V136 Male-body	V137 Head	V138 Male-body	V139 Female-body	V140 Female-body	V141 Female-body	V142 Female-body	V144 OSC	V145 S2	V146 S1	V147 1182-2H	V148 mbn2	GSM1528798 follicle cells
.................................................................................ATAAGGTAGAGAAATTGATGCTGTC...............................................................................................................................	25	1	41535.00	41535	0	0	0	0	0	5	0	0	0	1	1	20	1	0	0	0	0	0	0	0	22	2	3	0	0	0	0	0	0	0	0	0	0	0	0	43	170	33	30	49	60	35	158	36	20	44	279	41	93	35	115	85	25	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	2	2	1	1	3	0	3	3	0	0	0	0	0	9	0	0	138	0	2	23	0	0	4	2	9	1	0	0	8	0	5	2	2	45	0	0	2	7	0	0	0	0	0	0	1	0	0	0	0	0	0	0	2	3	2	1	0	0	0	0	5	1	7	0	0	4	4	75	206	390	203	312	1057	74	73	47	50	0	0	0	0	0	14	0	3	2	0	15	0	2	0	2	1	0	1	13	41	0	0	2	7	1	64	0	4	0	14	0	0	3908	76	5947	97	348	0	2	711	2	3005	68	3	0	0	0	1	53	100	9	5	9	10	0	4	9	9	4	357	960	15	875	261	162	466	812	254	66	408	319	0	1	3	0	0	0	0	0	9	6	0	4	0	0	1	6	0	90	1	0	70	0	0	0	0	1	2	0	0	3	0	0	1	0	0	1	1	0	0	1	0	0	155	1158	16	73	20	177	2	38	0	4	0	0	1	3	3	7	5	35	48	1132	75	6	40	28	105	37	133	563	7017	880	1045	0	0	15	1505	243	7	1	21	2	1	0	3	6	1	3	0	449	19	953	116	192	249	348	647	1	7	15	5	0
.................................................................................ATAAGGTAGAGAAATTGATGCTGTCT..............................................................................................................................	26	1	13984.00	13984	0	0	0	0	0	0	0	0	0	0	0	4	0	0	0	0	0	0	0	0	2	1	3	0	0	0	0	0	0	0	0	0	0	0	0	15	55	10	13	13	20	9	30	20	0	12	104	17	22	8	16	33	16	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	21	0	0	0	0	0	0	3	0	0	0	0	0	0	0	0	0	12	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	1	0	0	18	56	113	32	68	105	21	11	16	13	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	3	2	0	0	1	1	0	0	0	0	0	0	0	0	688	17	1942	23	26	0	0	157	1	1024	25	0	1	0	0	0	11	37	0	3	0	0	0	0	0	12	0	82	166	4	122	80	63	98	307	114	33	205	84	2	0	0	0	0	0	0	0	1	1	0	1	0	0	1	2	0	47	0	0	15	0	0	0	0	0	0	0	0	1	0	1	0	0	0	0	0	0	0	1	0	0	143	858	10	79	6	91	12	38	0	0	0	0	0	0	0	1	0	7	42	312	26	0	12	18	61	37	112	158	3632	288	343	0	2	15	641	157	0	0	5	0	1	0	0	2	1	0	0	71	2	154	38	26	50	52	151	0	3	4	0	0
.................................................................................ATAAGGTAGAGAAATTGATGCTG.................................................................................................................................	23	1	12620.00	12620	0	0	0	0	0	2	0	0	0	0	0	4	0	0	0	0	0	0	0	0	3	0	1	24	0	0	0	0	0	0	0	0	0	0	0	12	49	10	7	14	32	8	71	14	12	8	94	10	25	15	27	22	8	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	1	0	0	0	0	0	0	0	0	0	0	3	0	0	12	0	0	3	0	0	0	0	4	2	0	0	0	0	0	0	0	6	0	0	0	0	0	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	1	1	0	0	27	70	83	29	62	196	27	18	16	16	0	0	0	0	0	4	0	0	4	0	4	1	0	0	2	0	0	0	4	6	0	0	0	0	4	6	0	0	0	13	5	1	1393	21	2477	28	32	0	0	339	0	1328	49	0	0	0	0	0	9	23	0	4	5	1	0	0	17	7	5	150	321	7	254	4	8	19	112	23	11	14	15	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	27	0	0	8	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	2	0	0	59	425	11	34	7	90	4	22	0	2	0	0	0	2	9	3	4	3	14	239	22	1	12	2	19	11	58	161	1793	205	282	0	0	4	383	92	0	0	4	0	0	0	0	0	0	1	0	126	2	375	64	36	82	66	61	1	3	1	3	0
.................................................................................ATAAGGTAGAGAAATTGATGCTGT................................................................................................................................	24	1	7426.00	7426	0	0	0	0	0	0	0	0	0	0	0	10	1	0	0	0	0	0	0	0	5	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	52	7	6	11	12	4	65	13	5	10	73	9	24	5	21	16	13	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	0	0	0	5	0	0	0	0	0	0	3	0	0	90	1	3	13	0	0	2	7	2	1	0	0	2	0	1	2	1	36	0	0	2	9	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	0	0	0	0	1	0	0	0	10	24	63	22	60	53	11	1	3	4	0	0	0	0	0	0	0	5	0	2	0	0	0	0	0	0	0	0	0	1	0	0	1	1	0	7	0	3	0	4	0	0	382	21	622	27	5	0	0	54	1	391	8	0	0	0	0	0	12	27	4	1	1	0	0	0	1	0	0	109	227	1	183	25	29	78	284	49	13	56	53	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	12	0	0	8	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	77	475	13	47	24	79	5	25	0	0	0	0	0	1	1	0	1	2	17	88	21	1	19	14	29	17	56	42	1977	69	49	0	0	3	439	115	1	0	0	0	0	0	0	1	0	0	0	14	2	49	22	21	28	33	32	0	6	1	0	0
.................................................................................ATAAGGTAGAGAAATTGATGCT..................................................................................................................................	22	1	2870.00	2870	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	0	29	0	0	0	0	0	0	0	0	0	0	0	3	14	1	4	2	5	4	29	4	3	0	21	0	6	10	12	7	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	2	0	0	0	0	0	0	0	0	1	0	1	0	0	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	4	12	15	9	16	22	3	5	7	7	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	1	1	0	0	0	4	3	4	59	9	368	7	18	0	0	52	0	288	13	0	0	0	0	0	2	9	0	2	1	1	0	0	4	2	0	31	71	3	138	5	10	37	144	20	8	23	28	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	20	92	2	8	5	21	1	7	0	0	0	0	0	3	0	1	0	1	3	64	10	0	3	3	8	6	22	14	582	17	16	0	0	1	130	31	2	0	2	0	0	0	0	0	0	0	1	16	0	61	19	6	16	15	23	0	1	0	0	0
.................................................................................ATAAGGTAGAGAAATTGATG....................................................................................................................................	20	1	1202.00	1202	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	74	0	0	0	0	0	0	0	0	0	0	0	2	0	1	0	2	2	0	12	1	0	0	12	1	4	1	7	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	3	2	2	4	32	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	2	0	0	60	1	173	2	3	0	0	50	0	146	13	0	0	0	0	0	1	2	4	0	0	0	0	0	0	0	0	14	24	0	30	1	2	1	28	1	0	1	7	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	17	1	0	0	8	2	1	0	0	0	0	0	0	2	1	0	0	1	42	2	0	2	1	2	1	7	16	159	36	76	0	0	0	31	6	0	0	2	0	0	0	0	0	0	0	0	9	1	23	2	2	0	1	4	0	0	0	1	0
.................................................................................ATAAGGTAGAGAAATTGATGC...................................................................................................................................	21	1	1111.00	1111	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	41	0	0	0	0	0	0	0	0	0	0	0	1	5	0	1	1	2	1	14	0	1	0	8	1	1	0	9	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	2	0	0	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	2	6	9	3	5	25	2	0	1	0	0	0	0	0	0	2	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	0	39	4	135	3	0	0	0	26	0	101	9	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	16	40	1	59	5	0	6	25	7	0	5	7	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	5	0	0	1	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	8	34	0	4	0	10	0	0	0	0	0	0	0	0	0	0	0	0	1	34	6	1	0	0	1	5	7	7	171	6	15	0	0	0	58	12	0	0	0	0	0	0	0	0	0	0	0	12	0	28	8	4	9	6	14	1	2	0	0	0
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.................................................................................ATAAGGTAGAGAAATTGATGCTA.................................................................................................................................	23	1	10.00	10	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.................................................................................ATAAGGTAGAGAAATTGATT....................................................................................................................................	20	1	9.00	9	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	5	0	0	0	0	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.......................................................................................TAGAGAAATTGATGCTGTCT..............................................................................................................................	20	1	9.00	9	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	3	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...................................................................................AAGGTAGAGAAATTGATGCTGTC...............................................................................................................................	23	1	9.00	9	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

droSim2

CTCGACTCGA---CTTGAATCTA------C----------AACT--ACCTCGAT-------------GGGGCGCTCAGGCTATAAGGTAGAGAAATTGATGTTGTCTAC---------ACTAT--TCAGACTTCAGTTTCATTGCCTTTCAATTTGTTTGCCCC-CCT--GCAACAG-------------------------------TGA-------------------TAG	Size	Hit Count	Total Norm	Total	M023 Head	M024 Male-body	M025 Embryo	M053 Female-body	O001 Testis	O002 Head	SRR553485 Ovary	SRR553486 Ovary	SRR553487 Ovary	SRR553488 Ovary	SRR618934 Ovary	V044 Embryo	SRR902008 Ovary	SRR902009 Testis	SRR1275487 Male larvae	SRR1275485 Male prepupae	SRR1275483 Male prepupae
.................................................................................ATAAGGTAGAGAAATTGATGTTGT................................................................................................................................	24	1	5415.00	5415	0	40	0	3	2	0	28	37	0	0	134	0	26	5145	0	0	0
.................................................................................ATAAGGTAGAGAAATTGATGTT..................................................................................................................................	22	1	4495.00	4495	1	22	0	3	97	1	36	72	1	0	168	3	15	4076	0	0	0
.................................................................................ATAAGGTAGAGAAATTGATGTTG.................................................................................................................................	23	1	3519.00	3519	0	2	0	1	64	1	10	13	0	0	57	0	15	3356	0	0	0
.................................................................................ATAAGGTAGAGAAATTGATGTTGTC...............................................................................................................................	25	1	2980.00	2980	0	243	19	5	65	1	22	39	0	0	122	6	14	2444	0	0	0
.................................................................................ATAAGGTAGAGAAATTGATGTTGTCT..............................................................................................................................	26	1	663.00	663	0	52	2	1	1	0	16	37	1	0	95	1	5	452	0	0	0
.................................................................................ATAAGGTAGAGAAATTGATGT...................................................................................................................................	21	1	241.00	241	0	2	0	0	47	3	3	3	0	0	17	0	1	165	0	0	0
.................................................................................ATAAGGTAGAGAAATTGATG....................................................................................................................................	20	1	190.00	190	0	3	0	0	114	1	0	0	0	0	10	0	0	62	0	0	0
.................................................................................ATAAGGTAGAGAAATTGATGTTT.................................................................................................................................	23	1	168.00	168	0	0	0	0	0	0	1	1	0	0	2	0	0	164	0	0	0
.................................................................................ATAAGGTAGAGAAATTGA......................................................................................................................................	18	1	99.00	99	0	0	0	0	95	0	0	0	0	0	2	0	0	2	0	0	0
.................................................................................ATAAGGTAGAGAAATTGATGTTGTT...............................................................................................................................	25	1	91.00	91	0	1	0	0	0	0	0	0	0	0	0	0	0	90	0	0	0
.................................................................................ATAAGGTAGAGAAATTGATGTTA.................................................................................................................................	23	1	88.00	88	0	0	0	0	0	0	0	0	0	0	0	0	0	88	0	0	0
.................................................................................ATAAGGTAGAGAAATTGAT.....................................................................................................................................	19	1	82.00	82	0	0	0	0	74	2	0	0	0	0	1	0	0	5	0	0	0
.................................................................................................................................ACTTCAGTTTCATTGCCTTTCA..................................................................................	22	1	61.00	61	0	1	0	0	38	0	1	0	0	0	0	0	0	21	0	0	0
.................................................................................ATAAGGTAGAGAAATT........................................................................................................................................	16	1	38.00	38	0	0	0	0	0	0	0	0	0	0	38	0	0	0	0	0	0
.................................................................................ATAAGGTAGAGAAATTGATGTTGTCA..............................................................................................................................	26	1	30.00	30	0	0	0	0	0	0	0	0	0	0	1	0	0	29	0	0	0
.................................................................................................................................ACTTCAGTTTCATTGCCTTTC...................................................................................	21	1	19.00	19	0	0	0	0	18	0	0	0	0	0	0	0	0	1	0	0	0
..................................................................................................................................CTTCAGTTTCATTGCCTTTCA..................................................................................	21	1	18.00	18	0	3	0	0	4	0	0	1	0	0	0	0	0	10	0	0	0
.................................................................................................................................ACTTCAGTTTCATTGCCT......................................................................................	18	1	17.00	17	0	0	0	0	16	1	0	0	0	0	0	0	0	0	0	0	0
.................................................................................ATAAGGTAGAGAAATTGATGTTGTA...............................................................................................................................	25	1	17.00	17	0	0	0	0	0	0	0	0	0	0	0	0	0	17	0	0	0
..................................................................................TAAGGTAGAGAAATTGATGTTGTCT..............................................................................................................................	25	1	15.00	15	0	2	0	0	0	0	0	0	0	0	2	0	0	11	0	0	0
.................................................................................ATAAGGTAGAGAAATTGATGTTGA................................................................................................................................	24	1	14.00	14	0	0	0	0	0	0	0	0	0	0	0	0	0	14	0	0	0
..................................................................................................................................CTTCAGTTTCATTGCCTTTCAA.................................................................................	22	1	12.00	12	0	1	0	0	5	0	0	1	0	0	0	0	0	5	0	0	0
..................................................................................TAAGGTAGAGAAATTGATGTTG.................................................................................................................................	22	1	10.00	10	0	0	0	0	0	0	0	0	0	0	1	0	0	9	0	0	0
.................................................................................................................................ACTTCAGTTTCATTGCCTTTCAA.................................................................................	23	1	10.00	10	0	0	0	0	0	0	0	1	0	0	0	0	0	9	0	0	0
.................................................................................ATAAGGTAGAGAAATTGATGTA..................................................................................................................................	22	1	10.00	10	0	0	0	0	0	0	0	0	0	0	0	0	0	10	0	0	0
..................................................................................TAAGGTAGAGAAATTGATGTTGTC...............................................................................................................................	24	1	9.00	9	0	0	0	0	0	0	1	0	0	0	0	0	0	8	0	0	0
..................................................................................TAAGGTAGAGAAATTGATGTTGT................................................................................................................................	23	1	9.00	9	0	0	0	0	0	0	0	1	0	0	1	0	0	7	0	0	0
.................................................................................ATAAGGTAGAGAAAT.........................................................................................................................................	15	1	8.00	8	0	0	0	0	0	0	0	0	0	0	8	0	0	0	0	0	0
.................................................................................ATAAGGTAGAGAAATTGATGTTGTCC..............................................................................................................................	26	1	7.00	7	0	5	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0
.................................................................................ATAAGGTAGAGAAATTGATGTTAA................................................................................................................................	24	1	6.00	6	0	0	0	0	0	0	0	0	0	0	0	0	0	6	0	0	0
.................................................................................ATAAGGTAGAGAAATTGATGTG..................................................................................................................................	22	1	6.00	6	0	0	0	0	0	0	0	0	0	0	1	0	0	5	0	0	0
.................................................................................ATAAGGTAGAGAAATTGATGTTGC................................................................................................................................	24	1	5.00	5	0	2	0	0	0	0	0	0	0	0	1	0	0	2	0	0	0
..................................................................................................................................CTTCAGTTTCATTGCCTT.....................................................................................	18	1	5.00	5	0	0	0	0	5	0	0	0	0	0	0	0	0	0	0	0	0
.................................................................................................................................ACTTCAGTTTCATTGCCTT.....................................................................................	19	1	5.00	5	0	0	0	0	5	0	0	0	0	0	0	0	0	0	0	0	0
.................................................................................................................................ACTTCAGTTTCATTGCCTTT....................................................................................	20	1	5.00	5	0	0	0	0	3	0	0	0	0	0	0	0	0	2	0	0	0
.................................................................................ATAAGGTAGAGAAATTGATGTTGTCTT.............................................................................................................................	27	1	4.00	4	0	1	0	0	0	0	0	0	0	0	0	0	0	3	0	0	0
.................................................................................ATAAGGTAGAGAAATTGATGTTGTCTA.............................................................................................................................	27	1	4.00	4	0	0	0	0	1	0	0	0	0	0	0	0	0	3	0	0	0
.................................................................................ATAAGGTAGAGAAATTGATGTTGG................................................................................................................................	24	1	4.00	4	0	1	0	0	0	0	0	0	0	0	0	0	0	3	0	0	0
..................................................................................TAAGGTAGAGAAATTGATGTT..................................................................................................................................	21	1	3.00	3	0	0	0	0	0	0	0	0	0	0	0	0	0	3	0	0	0
.................................................................................ATAAGGTAGAGAAATTG.......................................................................................................................................	17	1	3.00	3	0	0	0	0	0	0	0	0	0	0	2	0	0	1	0	0	0
.................................................................................ATAAGGTAGAGAAATTGATGTTGTCTAA---------...................................................................................................................	28	1	3.00	3	0	0	0	0	0	0	0	0	0	0	0	0	0	3	0	0	0
.................................................................................ATAAGGTAGAGAAATTGATGTTC.................................................................................................................................	23	1	3.00	3	0	0	0	0	0	0	0	0	0	0	1	0	0	2	0	0	0
.................................................................................ATAAGGTAGAGAAATTGATGTTGTG...............................................................................................................................	25	1	3.00	3	0	0	0	0	0	0	0	0	0	0	0	0	0	3	0	0	0
.................................................................................ATAAGGTAGAGAAATTGAAA....................................................................................................................................	20	1	3.00	3	0	0	0	0	0	0	0	0	0	0	0	0	0	3	0	0	0
..................................................................................................................................CTTCAGTTTCATTGCCTTTC...................................................................................	20	1	3.00	3	0	0	0	0	1	0	0	0	0	0	0	0	0	2	0	0	0
...................................................................................AAGGTAGAGAAATTGATG....................................................................................................................................	18	1	2.00	2	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0
.................................................................................ATAAGGTAGAGAAATTGATGTTGTAA..............................................................................................................................	26	1	2.00	2	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0
.................................................................................ATAAGGTAGAGAAATTGATGTTGTCG..............................................................................................................................	26	1	2.00	2	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
....................................................................................AGGTAGAGAAATTGATGTT..................................................................................................................................	19	1	2.00	2	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0
.................................................................................ATAAGGTAGAGAAATTGATA....................................................................................................................................	20	1	2.00	2	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0
.................................................................................ATAAGGTAGAGAAATTGATT....................................................................................................................................	20	1	2.00	2	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0
....................................................................................AGGTAGAGAAATTGATGTTGT................................................................................................................................	21	1	2.00	2	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0
..................................................................................TAAGGTAGAGAAATTGATGTTGTA...............................................................................................................................	24	1	2.00	2	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0
.................................................................................ATAAGGTAGAGAAATTGATGTTGAAA..............................................................................................................................	26	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
...................................................................................AAGGTAGAGAAATTGATGTTG.................................................................................................................................	21	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0
.................................................................................ATAAGGTAGAGAAATTGATGTTGTCTTT---------...................................................................................................................	28	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
........................................................................................AGAGAAATTGATGTTGTCT..............................................................................................................................	19	1	1.00	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
..................................................................................TAAGGTAGAGAAATTGATG....................................................................................................................................	19	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0
.................................................................................ATAAGGTAGAGAAATTGATGTTAG................................................................................................................................	24	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
........................................................................................AGAGAAATTGATGTTG.................................................................................................................................	16	1	1.00	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
..................................................................................................................................CTTCAGTTTCATTGCCTTTCAAA................................................................................	23	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
...................................................................................AAGGTAGAGAAATTGATGTTGTCAT.............................................................................................................................	25	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
.................................................................................ATAAGGTAGAGAAATTGATGTTGGAC..............................................................................................................................	26	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
........................................................................................AGAGAAATTGATGTTGT................................................................................................................................	17	1	1.00	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
.................................................................................ATAAGGTAGAGAAATTGATGTGT.................................................................................................................................	23	1	1.00	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
........................................................................................AGAGAAATTGATGTTGTC...............................................................................................................................	18	1	1.00	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
...................................................................GGGGCGCTCAGGCTATAAGGTA................................................................................................................................................	22	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0
..................................................................................TAAGGTAGAGAAATTGATGTTGTCC..............................................................................................................................	25	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.............................................................................................................................TCAGACTTCAGTTTCATTGCCT......................................................................................	22	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.......................................................................................TAGAGAAATTGATGTTGTC...............................................................................................................................	19	1	1.00	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
...................................................................................................................................TTCAGTTTCATTGCCTTTCA..................................................................................	20	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
...................................................................................AAGGTAGAGAAATTGATGTTGTC...............................................................................................................................	23	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
....................................................................................AGGTAGAGAAATTGATGTTGTC...............................................................................................................................	22	1	1.00	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
..................................................................................................................................CTTCAGTTTCATTGCCTTT....................................................................................	19	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0
.......................................................................................TAGAGAAATTGATGTT..................................................................................................................................	16	1	1.00	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
.........................................................................................GAGAAATTGATGTTGTC...............................................................................................................................	17	1	1.00	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
..................................................................................................................................CTTCAGTTTCATTGCCTTTCT..................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
.......................................................................................TAGAGAAATTGATGTTGTCT..............................................................................................................................	20	1	1.00	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
....................................................................................AGGTAGAGAAATTGATGTTGTCATA---------...................................................................................................................	25	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
.......................................................................................TAGAGAAATTGATGTTGT................................................................................................................................	18	1	1.00	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
.................................................................................ATAAGGTAGAGAAATTGAA.....................................................................................................................................	19	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
................................................................................TATAAGGTAGAGAAATTGATGT...................................................................................................................................	22	1	1.00	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
...........................................................................................GAAATTGATGTTGTC...............................................................................................................................	15	2	0.50	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
.................................................................................ATAAGGTAGAGAAATA........................................................................................................................................	16	6	0.17	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0

droSec2

CTCGACTCGA---CTTGAATCTA------C----------AACT--ACCTCGAT-------------GGGGCATTCAGGCTATAAGGTAGAGAAATTGATGTTGTCTAC---------ACTAT--TCAGACTTCAGTTTCATTGCCTTTCAATTTGTTTGCCCC-CAT--GCAACAG-------------------------------TGG-------------------TAG	Size	Hit Count	Total Norm	Total	M054 Female-body	V113 Male-body	V114 Embryo	V115 Head
.................................................................................ATAAGGTAGAGAAATTGATGTT..................................................................................................................................	22	1	819.00	819	7	649	157	6
.................................................................................ATAAGGTAGAGAAATTGATGTTGT................................................................................................................................	24	1	764.00	764	2	697	61	4
.................................................................................ATAAGGTAGAGAAATTGATGTTGTC...............................................................................................................................	25	1	754.00	754	3	542	200	9
.................................................................................ATAAGGTAGAGAAATTGATGTTGTCT..............................................................................................................................	26	1	486.00	486	0	352	130	4
.................................................................................ATAAGGTAGAGAAATTGATGTTG.................................................................................................................................	23	1	393.00	393	4	376	10	3
.................................................................................ATAAGGTAGAGAAATTGATGT...................................................................................................................................	21	1	28.00	28	2	22	4	0
..................................................................................TAAGGTAGAGAAATTGATGTTGTCT..............................................................................................................................	25	1	9.00	9	1	6	2	0
.................................................................................ATAAGGTAGAGAAATTGATG....................................................................................................................................	20	1	7.00	7	0	7	0	0
.................................................................................ATAAGGTAGAGAAATTGATGTTT.................................................................................................................................	23	1	6.00	6	0	6	0	0
.................................................................................ATAAGGTAGAGAAATTGATGTTGTT...............................................................................................................................	25	1	6.00	6	0	5	1	0
..................................................................................TAAGGTAGAGAAATTGATGTTGTC...............................................................................................................................	24	1	4.00	4	0	4	0	0
.................................................................................ATAAGGTAGAGAAATTGATGTTGTCTT.............................................................................................................................	27	1	4.00	4	0	2	2	0
..................................................................................TAAGGTAGAGAAATTGATGTTGT................................................................................................................................	23	1	4.00	4	0	3	1	0
..................................................................................TAAGGTAGAGAAATTGATGTTG.................................................................................................................................	22	1	3.00	3	0	3	0	0
.................................................................................ATAAGGTAGAGAAATTGATGTTGG................................................................................................................................	24	1	1.00	1	0	1	0	0
.................................................................................ATAAGGTAGAGAAATTGATGTTGTTCT.............................................................................................................................	27	1	1.00	1	0	0	0	1
..................................................................................TAAGGTAGAGAAATTGATGTT..................................................................................................................................	21	1	1.00	1	0	0	1	0
.................................................................................ATAAGGTAGAGAAATTGA......................................................................................................................................	18	1	1.00	1	0	0	1	0
.................................................................................ATAAGGTAGAGAAATTGATGTTGTA...............................................................................................................................	25	1	1.00	1	0	1	0	0
.................................................................................ATAAGGTAGAGAAATTGATGTTGA................................................................................................................................	24	1	1.00	1	0	1	0	0
.................................................................................ATAAGGTAGAGAAATTGATGTTGTCTA.............................................................................................................................	27	1	1.00	1	0	1	0	0
.................................................................................ATAAGGTAGAGAAATTGATGTC..................................................................................................................................	22	1	1.00	1	0	1	0	0
....................................................................................AGGTAGAGAAATTGATGTT..................................................................................................................................	19	1	1.00	1	0	1	0	0

droYak3

CTCGACTCGA---CTCGAA--------TCC----------AACC--AACTTCAT-------------GGGGCACACAAGCTATAAGGTAGAGAAATTGTTGTCTTCCAC---------ACTAT--TCAGACATCAGTTTAACTATCTTTCAATTTGTTTGCCCC-CAC--GCAACAG-------------------------------TGA-------------------TAA	Size	Hit Count	Total Norm	Total	M026 Head	M043 Female-body	M056 Embryo	V046 Embryo	V052 Head	V058 Head	V120 Male-body	SRR1275488 Male larvae	SRR1275486 Male prepupae	SRR1275484 Male prepupae	GSM1528802 follicle cells
.................................................................................ATAAGGTAGAGAAATTGTTGTC..................................................................................................................................	22	1	1280.00	1280	30	51	23	1	1	16	1158	0	0	0	0
.................................................................................ATAAGGTAGAGAAATTGTTGTCT.................................................................................................................................	23	1	633.00	633	13	14	4	0	0	2	600	0	0	0	0
.................................................................................ATAAGGTAGAGAAATTGTTGT...................................................................................................................................	21	1	129.00	129	1	18	1	0	0	2	107	0	0	0	0
..................................................................................TAAGGTAGAGAAATTGTTGTCT.................................................................................................................................	22	1	45.00	45	0	2	0	0	0	0	43	0	0	0	0
.................................................................................ATAAGGTAGAGAAATTGTTG....................................................................................................................................	20	1	23.00	23	0	2	0	0	0	1	20	0	0	0	0
.................................................................................ATAAGGTAGAGAAATTGTTGTCC.................................................................................................................................	23	1	5.00	5	2	0	1	0	0	0	2	0	0	0	0
.................................................................................ATAAGGTAGAGAAATTGTTGTCA.................................................................................................................................	23	1	4.00	4	0	1	0	0	0	0	3	0	0	0	0
..................................................................................TAAGGTAGAGAAATTGTTGTC..................................................................................................................................	21	1	2.00	2	0	2	0	0	0	0	0	0	0	0	0
.................................................................................ATAAGGTAGAGAAATTGTTGTCTT................................................................................................................................	24	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
..................................................................................TAAGGTAGAGAAATTGTTGTCTA................................................................................................................................	23	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
.................................................................................ATAAGGTAGAGAAATTGT......................................................................................................................................	18	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0
................................................................................TATAAGGTAGAGAAATTGTTGTCT.................................................................................................................................	24	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
...................................................................................AAGGTAGAGAAATTGTTGTC..................................................................................................................................	20	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0
.................................................................................ATAAGGTAGAGAAATTGTTGTT..................................................................................................................................	22	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0
....................................................................................AGGTAGAGAAATTGTTGTC..................................................................................................................................	19	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
..................................................................................................................................CATCAGTTTAACTATCTTTCA..................................................................................	21	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
...................................................................................AAGGTAGAGAAATTGTTGTCT.................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
.................................................................................ATAAGGTAGAGAAATTGTT.....................................................................................................................................	19	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
.................................................................................ATAAGGTAGAGAAATTGTTGTCG.................................................................................................................................	23	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0

droEre2

CTCGGCTCGA-------AT--------C-C----------CAGT--ACCTCCTT-------------GGGGCACTCAAGCTATAAGGTAGAAAAATTGTTGTCTTCCAC---------ACTAT--TCGGACTGCAGTTTAACTATCTTTCAATTTGTTTGCCCC-CAC--GCAACAG-------------------------------TGA-------------------CAA	Size	Hit Count	Total Norm	Total	M055 Female-body	M057 Embryo	V060 Head	V040 Embryo	V107 Male-body	V108 Head	GSM1528801 follicle cells
.................................................................................ATAAGGTAGAAAAATTGTTGTC..................................................................................................................................	22	1	7500.00	7500	22	11	35	3	6700	729	0
.................................................................................ATAAGGTAGAAAAATTGTTGT...................................................................................................................................	21	1	605.00	605	3	1	1	0	549	51	0
.................................................................................ATAAGGTAGAAAAATTGTTGTCT.................................................................................................................................	23	1	300.00	300	7	0	4	0	258	31	0
.................................................................................ATAAGGTAGAAAAATTGTTG....................................................................................................................................	20	1	82.00	82	1	0	1	0	71	9	0
..................................................................................TAAGGTAGAAAAATTGTTGTCT.................................................................................................................................	22	1	68.00	68	1	0	0	0	65	2	0
.................................................................................ATAAGGTAGAAAAATTGTTGTCA.................................................................................................................................	23	1	19.00	19	0	0	0	0	18	1	0
..................................................................................TAAGGTAGAAAAATTGTTGTC..................................................................................................................................	21	1	7.00	7	1	0	0	0	5	1	0
...................................................................................AAGGTAGAAAAATTGTTGTC..................................................................................................................................	20	1	6.00	6	0	0	0	0	6	0	0
.................................................................................ATAAGGTAGAAAAATTGTC.....................................................................................................................................	19	1	6.00	6	0	0	0	0	6	0	0
.................................................................................ATAAGGTAGAAAAATTGTTGTT..................................................................................................................................	22	1	4.00	4	0	0	0	0	4	0	0
.................................................................................ATAAGGTAGAAAAATTGTTGTA..................................................................................................................................	22	1	3.00	3	0	0	0	0	1	2	0
.................................................................................ATAAGGTAGAAAAATTGTTGTCC.................................................................................................................................	23	1	3.00	3	0	0	0	0	3	0	0
..................................................................................TAAGGTAGAAAAATTGTTGTCTT................................................................................................................................	23	1	1.00	1	0	0	0	0	0	1	0
..................................................................................................................................CTGCAGTTTAACTATCTTTCA..................................................................................	21	1	1.00	1	0	0	0	0	1	0	0
.................................................................................ATAAGGTAGAAAAATTGTTGC...................................................................................................................................	21	1	1.00	1	0	0	0	0	1	0	0
..................................................................................TAAGGTAGAAAAATTGTTGT...................................................................................................................................	20	1	1.00	1	0	0	0	0	1	0	0
.................................................................................ATAAGGTAGAAAAATTGTTGTCTC................................................................................................................................	24	1	1.00	1	0	0	0	0	1	0	0
.................................................................................ATAAGGTAGAAAAATTGTTGTGA.................................................................................................................................	23	1	1.00	1	0	0	0	0	1	0	0
..................................................................................TAAGGTAGAAAAATTGTTGTCTTC...............................................................................................................................	24	1	1.00	1	0	0	0	0	1	0	0
.................................................................................ATAAGGTAGAAAAATTGTTT....................................................................................................................................	20	1	1.00	1	0	0	0	0	1	0	0
..................................................................................TAAGGTAGAAAAATTGTTGTCA.................................................................................................................................	22	1	1.00	1	1	0	0	0	0	0	0

dpe_318

scaffold_10:2201184-2201238 -

Known Ortholog

Testes-restricted

antisense_to_intron

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads