ID:

dpe_304

 Coordinate:

scaffold_13:198415-198472 +

 Confidence:

Known Ortholog

 Class:

Testes-restricted

 Genomic Locale:

intergenic

 [View on UCSC Genome Browser {Cornell Mirror}]
Legend: mature star mismatch in alignment mismatch in read

Predicted structure

-33.4 -33.3 -33.2

Flybase annnotation

intergenic

No Repeatable elements found

Sense Strand Reads

GTGCCTAAAGAGTTTCGGGACTACTCCATCTTATACGATTGAAGTACTAGCGGAGAACGCGTTGCTATTGGTGTCTTTGGAAATCCAAAAGCAGCGATGTTATTCGGCAGTACCACAATCAATATTTAGTGAGTGTTCCGGGCTGTTTTCTGCAGTGA

***********************************.(((((..(((((.((.((((((((((((((.((((..((....))...)))).))))))).)))).))).)))))))..)))))..************************************ 		Read size # Mismatch Hit Count Total Norm Total V111

male body		 V057

head

 V050

head

 M042

female body

 V042

embryo

 M021

embryo
.......................................................................................AAAGCAGCGATGTTATTCGG................................................... 20 0 1 55.00 55 48 3 3 1 0 0
.......................................................................................AAAGCAGCGATGTTATTCGGCA................................................. 22 0 1 40.00 40 17 9 8 5 0 1
.......................................................................................AAAGCAGCGATGTTATTCGGC.................................................. 21 0 1 31.00 31 16 8 5 2 0 0
.......................................................................................AAAGCAGCGATGTTATTCGGCAG................................................ 23 0 1 31.00 31 8 16 6 1 0 0
......................................................................................AAAAGCAGCGATGTTATTCGG................................................... 21 0 1 30.00 30 15 7 8 0 0 0
......................................................................................AAAAGCAGCGATGTTATTCGGC.................................................. 22 0 1 20.00 20 4 12 3 1 0 0
......................................................................................AAAAGCAGCGATGTTATTCGGCA................................................. 23 0 1 9.00 9 1 5 3 0 0 0
.................................................GCGGAGAACGCGTTGCTATTGGTG..................................................................................... 24 0 1 5.00 5 5 0 0 0 0 0
.................................................GCGGAGAACGCGTTGCTATTGGT...................................................................................... 23 0 1 5.00 5 5 0 0 0 0 0
.......................................................................................AAAGCAGCGATGTTATTCGGCT................................................. 22 1 1 3.00 3 2 1 0 0 0 0
.................................................GCGGAGAACGCGTTGCTATTGG....................................................................................... 22 0 1 3.00 3 3 0 0 0 0 0
.......................................................................................AAAGCAGCGATGTTATTCGGCAGT............................................... 24 0 1 2.00 2 0 1 0 1 0 0
.......................................................................................AAAGCAGCGATGTTCTTCGGCA................................................. 22 1 1 2.00 2 0 2 0 0 0 0
................................................AGCGGAGAACGCGTTGCTATTG........................................................................................ 22 0 1 2.00 2 0 2 0 0 0 0
.......................................................................................AAAGCAGCGATGTTATTCGGT.................................................. 21 1 1 2.00 2 2 0 0 0 0 0
.......................................................................................AAAGCATCGATGTTATTCGGCAG................................................ 23 1 1 2.00 2 0 0 0 0 2 0
.................................................GCGGAGAACGCGTTGCTATTGGTGTCTT................................................................................. 28 0 1 2.00 2 2 0 0 0 0 0
......................................................................................AAAAGCAGCGATGTTATTCG.................................................... 20 0 1 2.00 2 2 0 0 0 0 0
.......................................................................................AAAGCAGCGATGTTATTAGGC.................................................. 21 1 1 1.00 1 0 1 0 0 0 0
......................................................................................AAAAGCAGCCATGTTATTCGGCAG................................................ 24 1 1 1.00 1 0 1 0 0 0 0
.................................................GCGGAGAACGCGTTGCTATTGGTGT.................................................................................... 25 0 1 1.00 1 1 0 0 0 0 0
.......................................................................................AAAGCAGCGATGTTATTCGGCATT............................................... 24 1 1 1.00 1 0 0 0 0 1 0
.......................................................................................AAAGCAGCGATGTTATTCAGCA................................................. 22 1 1 1.00 1 0 1 0 0 0 0
.......................................................................................AAAGAAGGGATGTTGTTCGGCA................................................. 22 3 1 1.00 1 0 1 0 0 0 0
.......................................................................................AAAGAAGCGATGTTATTCGGCAG................................................ 23 1 1 1.00 1 0 1 0 0 0 0
.......................................................................................AAAGCAGCGATGTTATTCGGCAGTTCCT........................................... 28 2 1 1.00 1 1 0 0 0 0 0
.......................................................................................AAAGCAGCGAGGTTATTCTGCAG................................................ 23 2 1 1.00 1 0 0 1 0 0 0
......................................................................................AAAAGCAGCGATGTTATTCGGT.................................................. 22 1 1 1.00 1 0 1 0 0 0 0
......................................................................................AAAAGCAGCGCTGTTATTCGGC.................................................. 22 1 1 1.00 1 1 0 0 0 0 0
.......................................................................................AAAGCAGCGATGTTATTCGGCATTT.............................................. 25 2 1 1.00 1 0 0 0 1 0 0
......................................................................................AAAAGCAGCGCTGTTATTCGG................................................... 21 1 1 1.00 1 0 1 0 0 0 0
......................................................................................AAAAGCAGCGATGTTATTCGC................................................... 21 1 1 1.00 1 0 1 0 0 0 0
.......................................................................................AAAGCAGCGATGTTCTTCGG................................................... 20 1 1 1.00 1 1 0 0 0 0 0
.......................................................................................AAAGCAGCGATGTTATTTGGCA................................................. 22 1 1 1.00 1 0 1 0 0 0 0
......................................................................................AAAAGCAGCGATGTTGTTCGGC.................................................. 22 1 1 1.00 1 1 0 0 0 0 0
..................................................CGGAGAACGCGTTGCTATTGG....................................................................................... 21 0 1 1.00 1 1 0 0 0 0 0
.......................................................................................AAAGTAGCGTTGTTATTCAGC.................................................. 21 3 1 1.00 1 0 1 0 0 0 0
.......................................................................................AAAGCAGCGATGTTATTCGGCAC................................................ 23 1 1 1.00 1 0 1 0 0 0 0
......................................................................................AAAAGCAGCGATGTTGTTCGG................................................... 21 1 1 1.00 1 1 0 0 0 0 0
.......................................................................................AAAGCAGCGATGTTATACGGCAG................................................ 23 1 1 1.00 1 0 0 1 0 0 0
.......................................................................................AAAGCACCGATGTTATCCGGCAT................................................ 23 3 1 1.00 1 0 1 0 0 0 0
......................................................................................AAAAGCAGCGATGGTATTCGC................................................... 21 2 1 1.00 1 0 1 0 0 0 0
.......................................................................................AAAGCAGCGATGTTATTCGGA.................................................. 21 1 1 1.00 1 1 0 0 0 0 0
................................................AGCGGAGAACGCGTTGCTA........................................................................................... 19 0 1 1.00 1 1 0 0 0 0 0
.......................................................................................AAAGCAGCGATGTCATTCGGCAG................................................ 23 1 1 1.00 1 0 0 1 0 0 0
......................................................................................AAAGGCAGCGATGTTATTCGGCA................................................. 23 1 1 1.00 1 1 0 0 0 0 0
.......................................................................................AAAGCAGCGATGTTATTCG.................................................... 19 0 1 1.00 1 1 0 0 0 0 0
......................................................................................AAAAGCAGCGATTTTATTCGGC.................................................. 22 1 1 1.00 1 0 1 0 0 0 0
......................................................................................AAAAGCAGCGATGTTATTCTGCA................................................. 23 1 1 1.00 1 0 1 0 0 0 0
......................................................................................AAAAACAGCGATGTTATTCG.................................................... 20 1 1 1.00 1 1 0 0 0 0 0
.......................................................................................AAAGCAGCGATGTTATTCGGCAT................................................ 23 1 1 1.00 1 1 0 0 0 0 0
......................................................................................AAAAGCAGCGATGTTATTCGGTT................................................. 23 2 1 1.00 1 0 1 0 0 0 0
.......................................................................................AAAGCAGCGATGTTATTCGGCAAA............................................... 24 2 1 1.00 1 1 0 0 0 0 0
.......................................................................................AAAGCAGCGATGTTATTCCGCA................................................. 22 1 1 1.00 1 0 0 0 1 0 0
.......................................................................................AAAGCATCGATGTTATTCGG................................................... 20 1 1 1.00 1 1 0 0 0 0 0
.................................................GCGGACAACGCGTTGCTATTGGTG..................................................................................... 24 1 1 1.00 1 0 0 1 0 0 0
.......................................................................................AAAGCAGCGATGTTATTCGGCAA................................................ 23 1 1 1.00 1 0 1 0 0 0 0
.......................................................................................AAAGCAGTGATGTTATTCTGCAG................................................ 23 2 1 1.00 1 0 1 0 0 0 0
.......................................................................................AAAGCAGAGATGTTATTCGGCTGA............................................... 24 3 1 1.00 1 0 1 0 0 0 0
......................................................................................AAAAGCAGCGATGTTATTCT.................................................... 20 1 1 1.00 1 1 0 0 0 0 0
.......................................................................................AAAGCAGCGATGTTATTCGACAGT............................................... 24 1 1 1.00 1 0 1 0 0 0 0
.......................................................................................AAAGCAGCGCTGTTACTCGGCAT................................................ 23 3 1 1.00 1 0 0 1 0 0 0
......................................................................................AAAAGCAGCGATGTTATTCGGCAC................................................ 24 1 1 1.00 1 0 0 0 1 0 0
..................................................CGGAGAACGCGTTGCTATTGGT...................................................................................... 22 0 1 1.00 1 1 0 0 0 0 0
.......................................................................................AAAGCAGCGATGTTATCCGGC.................................................. 21 1 1 1.00 1 0 0 1 0 0 0
.......................................................................................AAAGCAGAGATGTTATTCGGCA................................................. 22 1 1 1.00 1 0 1 0 0 0 0
......................................................................................AAAAGCCGCGATGGGATTCGG................................................... 21 3 2 0.50 1 0 1 0 0 0 0
......................................................................................AAAAGCAGCGATGTTATTGGA................................................... 21 2 2 0.50 1 0 1 0 0 0 0
......................................................................................AAAAGCAGCGATGTTGCTCCG................................................... 21 3 4 0.25 1 0 1 0 0 0 0
.......................................................................................AAAGCAGCGATGGTATTCGTG.................................................. 21 3 5 0.20 1 0 1 0 0 0 0
.............................................................................................................................TTAGGGCATGTTCCGGGCTG............. 20 3 6 0.17 1 0 1 0 0 0 0

Anti-sense strand reads

CACGGATTTCTCAAAGCCCTGATGAGGTAGAATATGCTAACTTCATGATCGCCTCTTGCGCAACGATAACCACAGAAACCTTTAGGTTTTCGTCGCTACAATAAGCCGTCATGGTGTTAGTTATAAATCACTCACAAGGCCCGACAAAAGACGTCACT

************************************.(((((..(((((.((.((((((((((((((.((((..((....))...)))).))))))).)))).))).)))))))..)))))..*********************************** 		Read size # Mismatch Hit Count Total Norm Total V057

head
.....................................................................CCACGGAAACCTTTAGGTC...................................................................... 19 2 2 0.50 1 1

Re-alignment of all predicted orthologs

Species Coordinate ID Type Alignment
droPer2 scaffold_13:198365-198522 + dpe_304 confident GTGCCTAAAGAGTTTCGGGACTACTCCATCTTATACGATTGAAGTACTAGCGGAGAACGCGTTGCTATTGGTGTCTTTGGAAATCCAAAAGCAGCGATGTTATTCGGCAGTACCACAATCAATATTTAGTGAGTGTTCCGGGCTGTTTTCTGCAGTGA
dp5 XL_group1e:8365877-8366033 - dps-mir-2522a Known_mirbase GTGCCTAGAGAGTTTCGGGACTGCTCCATTTTGTACGATTGAAGTACTAGCGGAGAATGCGTTGTTATTGGTGTCTGTGGAAATCCAAAAGCAGCGATGTTCTTCGGCAGTACCACAATCAATATTTAGTGAGTGTT-CGAGCTATATTCTTCAGTGA

Re-alignment of all predicted orthologs with sRNA reads

Species Read alignment
droPer2
GTGCCTAAAGAGTTTCGGGACTACTCCATCTTATACGATTGAAGTACTAGCGGAGAACGCGTTGCTATTGGTGTCTTTGGAAATCCAAAAGCAGCGATGTTATTCGGCAGTACCACAATCAATATTTAGTGAGTGTTCCGGGCTGTTTTCTGCAGTGASizeHit CountTotal NormTotalM021

Embryo		M042

Female-body		V042

Embryo		V050

Head		V057

Head		V111

Male-body
.......................................................................................AAAGCAGCGATGTTATTCGG...................................................20155.00550103348
.......................................................................................AAAGCAGCGATGTTATTCGGCA.................................................22140.00401508917
.......................................................................................AAAGCAGCGATGTTATTCGGC..................................................21131.00310205816
.......................................................................................AAAGCAGCGATGTTATTCGGCAG................................................23131.00310106168
......................................................................................AAAAGCAGCGATGTTATTCGG...................................................21130.00300008715
......................................................................................AAAAGCAGCGATGTTATTCGGC..................................................22120.00200103124
......................................................................................AAAAGCAGCGATGTTATTCGGCA.................................................2319.009000351
.................................................GCGGAGAACGCGTTGCTATTGGTG.....................................................................................2415.005000005
.................................................GCGGAGAACGCGTTGCTATTGGT......................................................................................2315.005000005
.......................................................................................AAAGCAGCGATGTTATTCGGCT.................................................2213.003000012
.................................................GCGGAGAACGCGTTGCTATTGG.......................................................................................2213.003000003
.......................................................................................AAAGCAGCGATGTTATTCGGCAGT...............................................2412.002010010
................................................AGCGGAGAACGCGTTGCTATTG........................................................................................2212.002000020
.......................................................................................AAAGCAGCGATGTTATTCGGT..................................................2112.002000002
.................................................GCGGAGAACGCGTTGCTATTGGTGTCTT.................................................................................2812.002000002
......................................................................................AAAAGCAGCGATGTTATTCG....................................................2012.002000002
.................................................GCGGAGAACGCGTTGCTATTGGTGT....................................................................................2511.001000001
......................................................................................AAAAGCAGCGATGTTATTCGGT..................................................2211.001000010
......................................................................................AAAAGCAGCGATGTTATTCGC...................................................2111.001000010
..................................................CGGAGAACGCGTTGCTATTGG.......................................................................................2111.001000001
.......................................................................................AAAGCAGCGATGTTATTCGGCAC................................................2311.001000010
.......................................................................................AAAGCAGCGATGTTATTCGGA..................................................2111.001000001
................................................AGCGGAGAACGCGTTGCTA...........................................................................................1911.001000001
.......................................................................................AAAGCAGCGATGTTATTCG....................................................1911.001000001
.......................................................................................AAAGCAGCGATGTTATTCGGCAT................................................2311.001000001
......................................................................................AAAAGCAGCGATGTTATTCGGTT.................................................2311.001000010
.......................................................................................AAAGCAGCGATGTTATTCGGCAAA...............................................2411.001000001
.......................................................................................AAAGCAGCGATGTTATTCGGCAA................................................2311.001000010
......................................................................................AAAAGCAGCGATGTTATTCT....................................................2011.001000001
......................................................................................AAAAGCAGCGATGTTATTCGGCAC................................................2411.001010000
..................................................CGGAGAACGCGTTGCTATTGGT......................................................................................2211.001000001
dp5
GTGCCTAGAGAGTTTCGGGACTGCTCCATTTTGTACGATTGAAGTACTAGCGGAGAATGCGTTGTTATTGGTGTCTGTGGAAATCCAAAAGCAGCGATGTTCTTCGGCAGTACCACAATCAATATTTAGTGAGTGTT-CGAGCTATATTCTTCAGTGASizeHit CountTotal NormTotalM022

Male-body		M040

Female-body		M059

Embryo		M062

Head		V043

Embryo		V051

Head		V112

Male-body		SRR902010

Ovary		SRR902011

Testis		SRR902012

CNS imaginal disc
.......................................................................................AAAGCAGCGATGTTCTTCGG...................................................20184.00840210105470100
.......................................................................................AAAGCAGCGATGTTCTTCGGCA.................................................22182.008203511059100120
.......................................................................................AAAGCAGCGATGTTCTTCGGC..................................................21164.00641221262613020
.......................................................................................AAAGCAGCGATGTTCTTCGGCAG................................................23149.004911921495080
......................................................................................AAAAGCAGCGATGTTCTTCGG...................................................21113.00130310005040
......................................................................................AAAAGCAGCGATGTTCTTCGGC..................................................22110.00100123031000
.......................................................................................AAAGCAGCGATGTTCTTCGGCAGT...............................................2419.0090311002020
.......................................................................................AAAGCAGCGATGTTCTTCG....................................................1916.0060110003010
.......................................................................................AAAGCAGCGATGTTCTTCGGCAA................................................2316.0060100022010
.......................................................................................AAAGCAGCGATGTTCTTCGGCT.................................................2213.0030100101000
......................................................................................AAAAGCAGCGATGTTCTTCG....................................................2013.0030000010020
................................................AGCGGAGAATGCGTTGTTATTGG.......................................................................................2312.0020000001010
.......................................................................................AAAGCAGCGATGTTCTTCGGCAT................................................2312.0020100000010
................................................AGCGGAGAATGCGTTGTTATTG........................................................................................2212.0021000000010
................................................................TTATTGGTGTCTGTGGAAATCCAAA.....................................................................2512.0020000000020
........................................................................................AAGCAGCGATGTTCTTCGGCAG................................................2212.0020200000000
.......................................................................................AAAGCAGCGATGTTCTTCGGCAGTT..............................................2512.0020200000000
........................................................................................AAGCAGCGATGTTCTTCGGCAC................................................2212.0020200000000
........................................................................................AAGCAGCGATGTTCTTCGGCA.................................................2112.0020200000000
.......................................................................................AAAGCAGCGATGTTCTTCGGCC.................................................2212.0021000000010
.......................................................................................AAAGCAGCGATGTTCTTCGGCAGTTT.............................................2611.0010100000000
.......................................................................................AAAGCAGCGATGTTCTTCGGCAGTCG.............................................2611.0010100000000
.......................................................................................AAAGCAGCGATGTTCTTCGGCAC................................................2311.0010100000000
.......................................................................................AAAGCAGCGATGTTCTTCGTT..................................................2111.0010000001000
......................................................................................AAAAGCAGCGATGTTCTTCGGT..................................................2211.0010000001000
.......................................................................................AAAGCAGCGATGTTCTTCGGTC.................................................2211.0010000001000
.......................................................................................AAAGCAGCGATGTTCTTCGGA..................................................2111.0010000000010
.......................................................................................AAAGCAGCGATGTTCTTCGGCAAA...............................................2411.0010000000010
.......................................................................................AAAGCAGCGATGTTCTTCGGT..................................................2111.0010000001000
.......................................................................................AAAGCAGCGATGTTCTTCGGTCT................................................2330.3311000000000

Generated: 10/20/2015 at 07:38 PM