dpe_298

--------------------------------------------------------------################################################################################################################################# TCGGCGATCAGCATTATATGTGCGGGGCTCTTGGGCATGTCGTCCTCATTTTTGCCACACAGATGGTAGGGTCTCCAGTTCTGCTACTGTTCTCGCATGTACGAGAATGTACTAGCCCTGATCCGAATCTGGGGCAGAAGCTGCAGCAGTGTGTGCCACATCGATCAGCGGACGGGGAAGCAGCAAGGCGT *********************************...((........((((((((...(((((....((((...(((..(((.(((.(((((((......)))))))))).)))..)))))))..))))).))))))))..............))*********************************	Read size	# Mismatch	Hit Count	Total Norm	Total	V111 male body	M042 female body	V050 head	V057 head
..................................................TTTGCCACACAGATGGTAGGG........................................................................................................................	21	0	2	45.50	91	68	17	6	0
......................................................CCACACAGATGGTAGGGTC......................................................................................................................	19	0	2	5.50	11	11	0	0	0
......................................................CCACACAGATGGTAGGGTCT.....................................................................................................................	20	0	2	2.50	5	5	0	0	0
............ATTATATGTGCGGGGCTCTT...............................................................................................................................................................	20	0	2	2.50	5	3	0	2	0
......................................................CCACACAGATGGTAGGGTCTCC...................................................................................................................	22	0	2	2.50	5	5	0	0	0
..................................................TTTGCCACACAGATGGTAGGGT.......................................................................................................................	22	0	2	2.00	4	3	1	0	0
....................................................TGCCACACAGATGGTAGGG........................................................................................................................	19	0	2	1.50	3	0	2	1	0
.......................GGGGCTCTTGGGCATGTCGTC...................................................................................................................................................	21	0	2	1.50	3	3	0	0	0
........................................................ACACAGATGGTAGGGTCTCCA..................................................................................................................	21	0	2	1.50	3	3	0	0	0
.............TTATATGTGCGGGGCTCTTGGG............................................................................................................................................................	22	0	2	1.50	3	2	1	0	0
................TATGTGCGGGGCTCTTGGGCTT.........................................................................................................................................................	22	1	2	1.00	2	2	0	0	0
.......................GGGGCTCTTGGGCATGTCG.....................................................................................................................................................	19	0	2	1.00	2	2	0	0	0
..................................................TTTGCCACACAGATGGTAGGGA.......................................................................................................................	22	1	2	1.00	2	2	0	0	0
..................................................TTTGCCACACAGATGGTAGG.........................................................................................................................	20	0	2	1.00	2	1	1	0	0
..............TATATGTGCGGGGCTCTTGG.............................................................................................................................................................	20	0	2	1.00	2	2	0	0	0
.....................GCGGGGCTCTTGGGCATGTCG.....................................................................................................................................................	21	0	2	1.00	2	2	0	0	0
.......................GGGGCTCTTGGGCATGTCGT....................................................................................................................................................	20	0	2	1.00	2	2	0	0	0
.......................GGGGCTCTTGGGCATGTC......................................................................................................................................................	18	0	2	0.50	1	0	1	0	0
.............TTATACGTGCGGGGCTCTT...............................................................................................................................................................	19	1	2	0.50	1	1	0	0	0
..GGCGATCAGCATTATATGTGCGGGG....................................................................................................................................................................	25	0	2	0.50	1	1	0	0	0
..................................................TTTGCTACACAGATGGTAGGG........................................................................................................................	21	1	2	0.50	1	1	0	0	0
...................................................TTGCCACACAGATGGTAGGG........................................................................................................................	20	0	2	0.50	1	1	0	0	0
......................................................CCACACAGATGGTTGGGTCT.....................................................................................................................	20	1	2	0.50	1	1	0	0	0
.......TCAGCATTATATGTGCGGGGCTT.................................................................................................................................................................	23	1	2	0.50	1	1	0	0	0
..................................................TTTGCCACACAGATGTTAGGG........................................................................................................................	21	1	2	0.50	1	1	0	0	0
...................................................TTGCCACACAGATGGTAGGGT.......................................................................................................................	21	0	2	0.50	1	1	0	0	0
.............TTATATGTGCGGGGCTCTTGG.............................................................................................................................................................	21	0	2	0.50	1	1	0	0	0
..............TATATGTGCGGGGCTCTTGGG............................................................................................................................................................	21	0	2	0.50	1	1	0	0	0
..................................................TTTGCCACACAGATGGCAGGG........................................................................................................................	21	1	2	0.50	1	1	0	0	0
...............ATATGTGCGGGGCTCTTGGGTT..........................................................................................................................................................	22	2	2	0.50	1	1	0	0	0
.........................................................CACAGATGGTAGGGTCTCCAA.................................................................................................................	21	1	2	0.50	1	0	1	0	0
..........GCATTATATGTGCGGGGA...................................................................................................................................................................	18	1	2	0.50	1	1	0	0	0
.......TCAGCATTATATGTGCGGGGTT..................................................................................................................................................................	22	1	2	0.50	1	1	0	0	0
...................................................TTTCCACACAGATGGTAGGG........................................................................................................................	20	1	2	0.50	1	1	0	0	0
.............................CTTGGGCATGTCGTCCTCATT.............................................................................................................................................	21	0	2	0.50	1	1	0	0	0
.........................................................CACAGATGGTAGGGTCTCCAG.................................................................................................................	21	0	2	0.50	1	1	0	0	0
................TATGTGCGGGGCTCTTGGG............................................................................................................................................................	19	0	2	0.50	1	1	0	0	0
..................................................TTTTCCACACAGATGGTAGGG........................................................................................................................	21	1	2	0.50	1	1	0	0	0
..........GCATTATATGTGCGGGGCTCC................................................................................................................................................................	21	1	2	0.50	1	1	0	0	0
..................................................TTTGCCACACAGATGGAAGGG........................................................................................................................	21	1	2	0.50	1	1	0	0	0
.........................................................CACAGATGGTAGGGTCTCCAGA................................................................................................................	22	1	2	0.50	1	0	0	0	1
..................................................TTTGCCACACAGATGGTATGG........................................................................................................................	21	1	2	0.50	1	1	0	0	0
......................................................CCACACAGATGGTAGGGTCTC....................................................................................................................	21	0	2	0.50	1	1	0	0	0
........................................................................................................................................................................TGGACGGGGAAAGAGCAAGG...	20	3	20	0.05	1	0	0	0	1

AGCCGCTAGTCGTAATATACACGCCCCGAGAACCCGTACAGCAGGAGTAAAAACGGTGTGTCTACCATCCCAGAGGTCAAGACGATGACAAGAGCGTACATGCTCTTACATGATCGGGACTAGGCTTAGACCCCGTCTTCGACGTCGTCACACACGGTGTAGCTAGTCGCCTGCCCCTTCGTCGTTCCGCA *********************************...((........((((((((...(((((....((((...(((..(((.(((.(((((((......)))))))))).)))..)))))))..))))).))))))))..............))*********************************	Read size	# Mismatch	Hit Count	Total Norm	Total	V057 head
...........................................................GTCTACAATCCCAGAGGC..................................................................................................................	18	2	6	0.17	1	1

Species	Coordinate	ID	Alignment
droPer2	scaffold_23:706862-707052 +	dpe_298	TCGGCGATCAGCATTATATGTGCGGGGCTCTTGGGCATGTCGTCCTCATTTTTGCCACACAGATGGTAGGGTCTCCAG-TT-CTGCTACTGTTCTCGCATGTACGAGAATGTACTAGCCCTGATCCGAATCTGGGGCAGAAGCTGCAGCAGTGTGTGCCACATCGATCAGCGGACGGGGAAGCAGCAAGGCGT
dp5	Unknown_singleton_3382:470-660 -		TCGGCGATCAGCATTATATGTGCGGGGCTCTTGGGCATGTCGTCCTCATTTTTGCCACACAGATGGTAGGGTCTCCAG-TT-CTGCTACTGTTCTCGCATGTACGAGAATGTACTAGCCCTGATCCGAATCTGGGGCAGAAGCTGCAGCAGTGTATGCCACATCGATCGGCGGACGGGGAAGCAGCACGGCAT
droAna3	scaffold_13337:2022354-2022446 +		CCGCAGATGAGCATTATCTGGGCCAGGCTCTTGGCGATATCCTCCACGTTGTCGTCGAACAGCTGATAGGGA-TCCAGCTTCCTTCTGCAATTG---------------------------------------------------------------------------------------------------
droBip1	scf7180000396569:2062548-2062639 +		CCGCAGATGAGCATTATCTGGGCCAGGCTCTTGGCGATGTCCTCCACGTTGTCGTCGAACAGCTGATAGGGA-TCCAGCTTCCTCCTGCAGTT----------------------------------------------------------------------------------------------------
droFic1	scf7180000454113:650329-650375 +		C-------------------------------------------------------------------------------------------------------------------------------------------------ATCTGCGAGTGCAACATCGATCAGCTGACTGGCGAGCAGTTCGGCG-
droBia1	scf7180000300910:1538531-1538577 -		C-------------------------------------------------------------------------------------------------------------------------------------------------ATCTGCGAGTGCAACATCGACCAGTTGACGGGCGAGCAGTTCGGCG-
droSim2	3l:1348501-1348598 +		CCGCAGATCAGCATTATCTGGGCCAGGCTCTTGGCTATGTCCTCCACATTATCGTCAAACAGTTGGTAGGGA-TCCAGCTTCCTCCTGCAGTTGCAGCA----------------------------------------------------------------------------------------------
droSec2	scaffold_11962:980-1077 +		CCGCAGATCAGCATTATCTGGGCCAGGCTCTTGGCTATGTCCTCCACATTATCGTCAAACAGTTGGTAGGGA-TCCAGCTTCCTCCTGCAGTTGCAGCA----------------------------------------------------------------------------------------------

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droPer2

TCGGCGATCAGCATTATATGTGCGGGGCTCTTGGGCATGTCGTCCTCATTTTTGCCACACAGATGGTAGGGTCTCCAG-TT-CTGCTACTGTTCTCGCATGTACGAGAATGTACTAGCCCTGATCCGAATCTGGGGCAGAAGCTGCAGCAGTGTGTGCCACATCGATCAGCGGACGGGGAAGCAGCAAGGCGT	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
..................................................TTTGCCACACAGATGGTAGGG..........................................................................................................................	21	2	45.50	91	0	17	0	6	0	68
......................................................CCACACAGATGGTAGGGTC........................................................................................................................	19	2	5.50	11	0	0	0	0	0	11
......................................................CCACACAGATGGTAGGGTCT.......................................................................................................................	20	2	2.50	5	0	0	0	0	0	5
............ATTATATGTGCGGGGCTCTT.................................................................................................................................................................	20	2	2.50	5	0	0	0	2	0	3
......................................................CCACACAGATGGTAGGGTCTCC.....................................................................................................................	22	2	2.50	5	0	0	0	0	0	5
..................................................TTTGCCACACAGATGGTAGGGT.........................................................................................................................	22	2	2.00	4	0	1	0	0	0	3
....................................................TGCCACACAGATGGTAGGG..........................................................................................................................	19	2	1.50	3	0	2	0	1	0	0
.......................GGGGCTCTTGGGCATGTCGTC.....................................................................................................................................................	21	2	1.50	3	0	0	0	0	0	3
........................................................ACACAGATGGTAGGGTCTCCA....................................................................................................................	21	2	1.50	3	0	0	0	0	0	3
.............TTATATGTGCGGGGCTCTTGGG..............................................................................................................................................................	22	2	1.50	3	0	1	0	0	0	2
.......................GGGGCTCTTGGGCATGTCG.......................................................................................................................................................	19	2	1.00	2	0	0	0	0	0	2
..................................................TTTGCCACACAGATGGTAGGGA.........................................................................................................................	22	2	1.00	2	0	0	0	0	0	2
..................................................TTTGCCACACAGATGGTAGG...........................................................................................................................	20	2	1.00	2	0	1	0	0	0	1
..............TATATGTGCGGGGCTCTTGG...............................................................................................................................................................	20	2	1.00	2	0	0	0	0	0	2
.....................GCGGGGCTCTTGGGCATGTCG.......................................................................................................................................................	21	2	1.00	2	0	0	0	0	0	2
.......................GGGGCTCTTGGGCATGTCGT......................................................................................................................................................	20	2	1.00	2	0	0	0	0	0	2
.......................GGGGCTCTTGGGCATGTC........................................................................................................................................................	18	2	0.50	1	0	1	0	0	0	0
..GGCGATCAGCATTATATGTGCGGGG......................................................................................................................................................................	25	2	0.50	1	0	0	0	0	0	1
...................................................TTGCCACACAGATGGTAGGG..........................................................................................................................	20	2	0.50	1	0	0	0	0	0	1
.......TCAGCATTATATGTGCGGGGCTT...................................................................................................................................................................	23	2	0.50	1	0	0	0	0	0	1
...................................................TTGCCACACAGATGGTAGGGT.........................................................................................................................	21	2	0.50	1	0	0	0	0	0	1
.............TTATATGTGCGGGGCTCTTGG...............................................................................................................................................................	21	2	0.50	1	0	0	0	0	0	1
..............TATATGTGCGGGGCTCTTGGG..............................................................................................................................................................	21	2	0.50	1	0	0	0	0	0	1
...............ATATGTGCGGGGCTCTTGGGTT............................................................................................................................................................	22	2	0.50	1	0	0	0	0	0	1
.........................................................CACAGATGGTAGGGTCTCCAA-..................................................................................................................	21	2	0.50	1	0	1	0	0	0	0
..........GCATTATATGTGCGGGGA.....................................................................................................................................................................	18	2	0.50	1	0	0	0	0	0	1
.............................CTTGGGCATGTCGTCCTCATT...............................................................................................................................................	21	2	0.50	1	0	0	0	0	0	1
.........................................................CACAGATGGTAGGGTCTCCAG-..................................................................................................................	21	2	0.50	1	0	0	0	0	0	1
................TATGTGCGGGGCTCTTGGG..............................................................................................................................................................	19	2	0.50	1	0	0	0	0	0	1
..........GCATTATATGTGCGGGGCTCC..................................................................................................................................................................	21	2	0.50	1	0	0	0	0	0	1
.........................................................CACAGATGGTAGGGTCTCCAG-A.................................................................................................................	22	2	0.50	1	0	0	0	0	1	0
......................................................CCACACAGATGGTAGGGTCTC......................................................................................................................	21	2	0.50	1	0	0	0	0	0	1

dp5

TCGGCGATCAGCATTATATGTGCGGGGCTCTTGGGCATGTCGTCCTCATTTTTGCCACACAGATGGTAGGGTCTCCAG-TT-CTGCTACTGTTCTCGCATGTACGAGAATGTACTAGCCCTGATCCGAATCTGGGGCAGAAGCTGCAGCAGTGTATGCCACATCGATCGGCGGACGGGGAAGCAGCACGGCAT	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
..................................................TTTGCCACACAGATGGTAGGG..........................................................................................................................	21	7	451.14	3158	0	553	0	22	0	230	1138	0	1215	0
..................................................TTTGCCACACAGATGGTAGG...........................................................................................................................	20	7	30.57	214	0	109	0	6	0	5	20	0	74	0
.............................CTTGGGCATGTCGTCCTCATT...............................................................................................................................................	21	7	10.71	75	1	6	0	0	0	1	6	0	61	0
............ATTATATGTGCGGGGCTCTTG................................................................................................................................................................	21	7	10.14	71	0	1	0	0	0	1	31	0	38	0
..................................................TTTGCCACACAGATGGTAGGGA.........................................................................................................................	22	7	6.86	48	0	5	0	0	0	2	19	0	22	0
...................................................TTGCCACACAGATGGTAGGG..........................................................................................................................	20	7	6.29	44	0	38	0	0	0	3	2	0	1	0
..................................................TTTGCCACACAGATGGTAGGGT.........................................................................................................................	22	7	5.71	40	0	3	0	0	0	2	19	0	16	0
.......................................................................TCTCCAG-TT-CTGCTACTGTTC...................................................................................................	21	6	4.83	29	1	2	0	1	0	0	2	0	23	0
.............TTATATGTGCGGGGCTCTTGG...............................................................................................................................................................	21	7	2.86	20	0	0	0	0	0	0	2	0	18	0
......................................................CCACACAGATGGTAGGGTC........................................................................................................................	19	7	2.43	17	1	1	0	0	0	0	15	0	0	0
.............TTATATGTGCGGGGCTCTTG................................................................................................................................................................	20	7	2.29	16	0	0	0	0	0	0	10	0	6	0
......................................................CCACACAGATGGTAGGGTCTC......................................................................................................................	21	7	2.29	16	2	2	0	0	0	1	9	0	2	0
..................................................TTTGCCACACAGATGGTAG............................................................................................................................	19	7	1.86	13	0	6	0	0	0	0	0	0	7	0
..............TATATGTGCGGGGCTCTTGGG..............................................................................................................................................................	21	7	1.71	12	0	0	0	0	0	0	2	0	10	0
..................................................TTTGCCACACAGATGGTAGGT..........................................................................................................................	21	7	1.71	12	0	0	0	0	0	0	2	0	10	0
..............TATATGTGCGGGGCTCTTGG...............................................................................................................................................................	20	7	1.57	11	0	0	0	0	0	0	2	0	9	0
...............ATATGTGCGGGGCTCTTGG...............................................................................................................................................................	19	7	1.57	11	0	0	0	0	0	0	3	0	8	0
.............................CTTGGGCATGTCGTCCTCATTC..............................................................................................................................................	22	7	1.57	11	0	1	0	0	0	1	0	0	9	0
..................................................TTTGCCACACAGATGGTAGGA..........................................................................................................................	21	7	1.43	10	0	2	0	0	0	1	1	0	6	0
.............TTATATGTGCGGGGCTCTTGGG..............................................................................................................................................................	22	7	1.43	10	0	1	0	0	0	0	2	0	7	0
.......TCAGCATTATATGTGCGGGG......................................................................................................................................................................	20	5	1.40	7	0	1	0	0	0	0	5	0	1	0
.................................................TTTTGCCACACAGATGGTAGG...........................................................................................................................	21	7	1.14	8	0	3	0	0	0	0	0	0	5	0
......................................................CCACACAGATGGTAGGGTCT.......................................................................................................................	20	7	1.14	8	1	0	0	0	0	0	7	0	0	0
.......................................................................TCTCCAG-TT-CTGCTACTGTT....................................................................................................	20	6	1.00	6	0	0	0	0	0	0	0	0	6	0
..................................................TTTGCCACACAGATGGTAGGGTT........................................................................................................................	23	7	0.86	6	0	0	0	0	0	0	4	0	2	0
..................................................TTTGCCACACAGATGGTAGGGAA........................................................................................................................	23	7	0.86	6	0	0	0	0	0	1	4	0	1	0
.............................CTTGGGCATGTCGTCCTCATTT..............................................................................................................................................	22	7	0.71	5	0	0	0	0	0	0	0	0	5	0
............ATTATATGTGCGGGGCTCTT.................................................................................................................................................................	20	7	0.71	5	0	0	0	1	0	0	1	0	3	0
...................................................TTGCCACACAGATGGTAGG...........................................................................................................................	19	7	0.57	4	0	4	0	0	0	0	0	0	0	0
.......................GGGGCTCTTGGGCATGTCG.......................................................................................................................................................	19	7	0.57	4	0	0	0	0	0	0	2	0	2	0
.............................CTTGGGCATGTCGTCCTC..................................................................................................................................................	18	7	0.57	4	3	0	0	0	0	0	0	0	1	0
.......................GGGGCTCTTGGGCATGTCGTC.....................................................................................................................................................	21	7	0.57	4	0	0	0	0	0	0	3	0	1	0
................................................TTTTTGCCACACAGATGGTAGG...........................................................................................................................	22	7	0.57	4	0	2	0	0	0	0	1	0	1	0
...............................TGGGCATGTCGTCCTCATTTT.............................................................................................................................................	21	7	0.57	4	0	0	0	0	0	0	0	0	4	0
..................................................TTTGCCACACAGATGGTAGGGC.........................................................................................................................	22	7	0.43	3	0	1	0	0	0	0	1	0	1	0
.......................................................................TCTCCAG-TT-CTGCTACTGTTT...................................................................................................	21	7	0.43	3	0	0	0	0	0	0	0	0	3	0
.................................................TTTTGCCACACAGATGGTAGGG..........................................................................................................................	22	7	0.43	3	0	1	0	0	0	0	1	0	1	0
...........................................................................................................................TCCGAATCTGGGGCAGAAGCTGCAG.............................................	25	7	0.43	3	0	0	0	0	0	0	0	0	3	0
................TATGTGCGGGGCTCTTGGGC.............................................................................................................................................................	20	7	0.43	3	0	0	0	0	0	0	0	0	3	0
...............................TGGGCATGTCGTCCTCATTTTT............................................................................................................................................	22	7	0.43	3	0	0	0	0	0	0	0	0	3	0
................TATGTGCGGGGCTCTTGGG..............................................................................................................................................................	19	7	0.43	3	0	0	0	0	0	0	2	0	1	0
.............TTATATGTGCGGGGCTCTT.................................................................................................................................................................	19	7	0.43	3	0	0	0	0	0	0	1	0	2	0
......................................................CCACACAGATGGTAGGGTCTT......................................................................................................................	21	7	0.43	3	1	0	0	0	0	0	2	0	0	0
..................................................TTTGCCACACAGATGGTAGGGTC........................................................................................................................	23	7	0.43	3	0	1	0	0	0	0	2	0	0	0
..................................................TTTGCCACACAGATGGTAGGAA.........................................................................................................................	22	7	0.43	3	0	0	0	0	0	0	0	0	3	0
...............ATATGTGCGGGGCTCTTGGGC.............................................................................................................................................................	21	7	0.43	3	0	0	0	0	0	0	0	0	3	0
...............ATATGTGCGGGGCTCTTGGG..............................................................................................................................................................	20	7	0.43	3	0	0	0	0	0	0	0	0	3	0
...................................................TTGCCACACAGATGGTAGGGTC........................................................................................................................	22	7	0.43	3	0	1	0	0	0	0	2	0	0	0
......................................................CCACACAGATGGTAGGGTCTCTC....................................................................................................................	23	7	0.29	2	0	0	0	0	0	0	0	0	2	0
.....................................................GCCACACAGATGGTAGGG..........................................................................................................................	18	7	0.29	2	0	2	0	0	0	0	0	0	0	0
............ATTATATGTGCGGGGCTCTTA................................................................................................................................................................	21	7	0.29	2	0	1	0	0	0	0	0	0	1	0
......................................................CCACACAGATGGTAGGGTCTCC.....................................................................................................................	22	7	0.29	2	0	0	0	0	0	0	2	0	0	0
............ATTATATGTGCGGGGCTCTC.................................................................................................................................................................	20	7	0.29	2	0	0	0	0	0	0	0	0	2	0
................................................TTTTTGCCACACAGATGGTAGGG..........................................................................................................................	23	7	0.29	2	0	1	0	0	0	0	1	0	0	0
..............................TTGGGCATGTCGTCCTCATTTC.............................................................................................................................................	22	7	0.29	2	0	0	0	0	0	0	0	0	2	0
.............................CTTGGGCATGTCGTCCTCAT................................................................................................................................................	20	7	0.29	2	0	1	0	0	0	1	0	0	0	0
......................................................CCACACAGATGGTAGGGTCA.......................................................................................................................	20	7	0.29	2	0	0	0	0	0	0	0	0	2	0
............ATTATATGTGCGGGGCTCTTT................................................................................................................................................................	21	7	0.29	2	0	0	0	0	0	0	1	0	1	0
............ATTATATGTGCGGGGCTCTTGA...............................................................................................................................................................	22	7	0.29	2	0	0	0	0	0	0	1	0	1	0
....................TGCGGGGCTCTTGGGCATGTC........................................................................................................................................................	21	7	0.29	2	0	0	0	0	0	0	1	0	1	0
....................................................TGCCACACAGATGGTAGGG..........................................................................................................................	19	7	0.29	2	0	2	0	0	0	0	0	0	0	0
....................................................TGCCACACAGATGGTAGGGTC........................................................................................................................	21	7	0.29	2	0	0	0	0	0	1	1	0	0	0
............ATTATATGTGCGGGGCTC...................................................................................................................................................................	18	7	0.29	2	2	0	0	0	0	0	0	0	0	0
.........AGCATTATATGTGCGGGGCTCTC.................................................................................................................................................................	23	5	0.20	1	0	0	0	1	0	0	0	0	0	0
.......TCAGCATTATATGTGCGGGGCT....................................................................................................................................................................	22	5	0.20	1	0	0	0	0	0	0	1	0	0	0
.........AGCATTATATGTGCGGGGC.....................................................................................................................................................................	19	5	0.20	1	0	0	0	0	0	0	0	0	1	0
.........AGCATTATATGTGCGGGGCTC...................................................................................................................................................................	21	5	0.20	1	0	1	0	0	0	0	0	0	0	0
.........AGCATTATATGTGCGGGGCTT...................................................................................................................................................................	21	5	0.20	1	0	0	0	0	0	0	1	0	0	0
.........AGCATTATATGTGCGGGGCTCT..................................................................................................................................................................	22	5	0.20	1	0	1	0	0	0	0	0	0	0	0
.......TCAGCATTATATGTGCGGG.......................................................................................................................................................................	19	5	0.20	1	0	0	0	0	0	1	0	0	0	0
.CGGCGATCAGCATTATATGTGCGGGG......................................................................................................................................................................	26	5	0.20	1	0	0	0	0	0	0	1	0	0	0
.......................................................................TCTCCAG-TT-CTGCTACTGTTCT..................................................................................................	22	6	0.17	1	0	0	0	0	0	0	0	0	1	0
.......................................................................TCTCCAG-TT-CTGCTACTGTTCC..................................................................................................	22	6	0.17	1	0	0	0	0	0	0	0	0	1	0
......................................................................GTCTCCAG-TT-CTGCTACTGTTCTCGC...............................................................................................	26	6	0.17	1	0	1	0	0	0	0	0	0	0	0
..................................................TTTGCCACACAGATGGTAGGGG.........................................................................................................................	22	7	0.14	1	0	0	0	0	0	0	0	0	1	0
.................................................................................................................................TCTGGGGCAGAAGCTGCAGCAT..........................................	22	7	0.14	1	0	0	0	0	0	0	0	0	1	0
.............TTATATGTGCGGGGCTCTTT................................................................................................................................................................	20	7	0.14	1	0	0	0	0	0	0	0	0	1	0
...........CATTATATGTGCGGGGCTC...................................................................................................................................................................	19	7	0.14	1	0	0	0	0	0	0	0	0	1	0
.......................................................................................................................................................TGTATGCCACATCGATCGGCG.....................	21	7	0.14	1	0	0	0	0	0	0	1	0	0	0
.............TTATATGTGCGGGGCTCTTA................................................................................................................................................................	20	7	0.14	1	0	0	0	0	0	0	1	0	0	0
..............................TTGGGCATGTCGTCCTCATTTTT............................................................................................................................................	23	7	0.14	1	0	0	0	0	0	0	0	0	1	0
.................................................TTTTGCCACACAGATGGTAG............................................................................................................................	20	7	0.14	1	0	0	0	0	0	0	0	0	1	0
..............................TTGGGCATGTCGTCCTCATTTTC............................................................................................................................................	23	7	0.14	1	0	0	0	0	0	0	0	0	1	0
.......................................................................................................................................................TGTATGCCACATCGATCGGCGGA...................	23	7	0.14	1	0	0	0	0	0	0	0	0	1	0
..................................................TTTGCCACACAGATGGTAGGGTTC.......................................................................................................................	24	7	0.14	1	0	0	0	0	0	0	1	0	0	0
..................................................TTTGCCACACAGATGGTAGGGTTA.......................................................................................................................	24	7	0.14	1	0	0	0	0	0	0	1	0	0	0
.....................................................GCCACACAGATGGTAGGGTC........................................................................................................................	20	7	0.14	1	0	0	0	0	0	0	1	0	0	0
...........CATTATATGTGCGGGGCTCTT.................................................................................................................................................................	21	7	0.14	1	0	0	0	0	0	0	1	0	0	0
..................................................TTTGCCACACAGATGGTAGGGAAA.......................................................................................................................	24	7	0.14	1	0	0	0	0	0	0	0	0	1	0
.......................................................................................................CGAGAATGTACTAGCCCTGAT.....................................................................	21	7	0.14	1	0	0	0	0	0	0	0	0	1	0
.............................CTTGGGCATGTCGTCCTCATTCC.............................................................................................................................................	23	7	0.14	1	0	0	0	0	0	0	1	0	0	0
..................................................TTTGCCACACAGATGGTAGGAAA........................................................................................................................	23	7	0.14	1	0	0	0	0	0	0	1	0	0	0
.................................................................................................................................TCTGGGGCAGAAGCTGCAGCAG..........................................	22	7	0.14	1	0	0	0	0	0	0	0	0	1	0
.............................CTTGGGCATGTCGTCCTCATTTTC............................................................................................................................................	24	7	0.14	1	0	0	0	0	0	0	0	0	1	0
...........CATTATATGTGCGGGGCTCTTGGGC.............................................................................................................................................................	25	7	0.14	1	0	0	0	0	0	0	0	0	1	0
..............................................................................................TCGCATGTACGAGAATGTACTAGCCCTGA......................................................................	29	7	0.14	1	0	0	0	0	0	0	0	0	1	0
..................................................TTTGCCACACAGATGGTA.............................................................................................................................	18	7	0.14	1	0	1	0	0	0	0	0	0	0	0
..............................TTGGGCATGTCGTCCTCATTC..............................................................................................................................................	21	7	0.14	1	0	1	0	0	0	0	0	0	0	0
.......................................................CACACAGATGGTAGGGTCTCC.....................................................................................................................	21	7	0.14	1	0	0	0	0	0	0	1	0	0	0
...............................TGGGCATGTCGTCCTCATTTTTC...........................................................................................................................................	23	7	0.14	1	0	0	0	0	0	0	0	0	1	0
.............................CTTGGGCATGTCGTCCTCATTA..............................................................................................................................................	22	7	0.14	1	0	0	0	0	0	0	0	0	1	0
..............................TTGGGCATGTCGTCCTCATTTTTCA..........................................................................................................................................	25	7	0.14	1	0	0	0	0	0	0	0	0	1	0
......................................................CCACACAGATGGTAGGGTCTCT.....................................................................................................................	22	7	0.14	1	0	0	0	0	0	0	1	0	0	0
..............................TTGGGCATGTCGTCCTCATTCC.............................................................................................................................................	22	7	0.14	1	0	0	0	0	0	0	0	0	1	0
.......................................................................................................................................................TGTATGCCACATCGATCGGCGGACG.................	25	7	0.14	1	1	0	0	0	0	0	0	0	0	0
......................................................CCACACAGATGGTAGGGTCTTT.....................................................................................................................	22	7	0.14	1	0	0	0	0	0	0	1	0	0	0
.............................CTTGGGCATGTCGTCCTCATTTT.............................................................................................................................................	23	7	0.14	1	0	0	0	0	0	0	0	0	0	1
..............TATATGTGCGGGGCTCTTG................................................................................................................................................................	19	7	0.14	1	0	0	0	0	0	0	0	0	1	0
......................................................CCACACAGATGGTAGGGTT........................................................................................................................	19	7	0.14	1	0	0	0	0	0	0	1	0	0	0
.............................................................................................................................................................CCACATCGATCGGCGGACG.................	19	7	0.14	1	0	0	0	0	0	0	0	0	1	0
.............................CTTGGGCATGTCGTCCTCATA...............................................................................................................................................	21	7	0.14	1	0	0	0	0	0	0	0	0	1	0
..............................TTGGGCATGTCGTCCTCAT................................................................................................................................................	19	7	0.14	1	0	0	0	0	0	0	1	0	0	0
...................................................TTGCCACACAGATGGTAG............................................................................................................................	18	7	0.14	1	0	1	0	0	0	0	0	0	0	0
.................................................................................................................................................CAGCAGTGTATGCCACATCGATCGGCG.....................	27	7	0.14	1	0	0	0	0	0	0	0	0	1	0
.............TTATATGTGCGGGGCTCTTGGT..............................................................................................................................................................	22	7	0.14	1	0	0	0	0	0	0	1	0	0	0
................TATGTGCGGGGCTCTTGGGCA............................................................................................................................................................	21	7	0.14	1	0	0	0	0	0	0	0	0	1	0
.................ATGTGCGGGGCTCTTGGG..............................................................................................................................................................	18	7	0.14	1	0	0	0	0	0	0	0	0	1	0
..............................TTGGGCATGTCGTCCTCATT...............................................................................................................................................	20	7	0.14	1	0	1	0	0	0	0	0	0	0	0
.......................GGGGCTCTTGGGCATGTCGT......................................................................................................................................................	20	7	0.14	1	0	0	0	0	0	1	0	0	0	0
.............................CTTGGGCATGTCGTCCTCATTTTTT...........................................................................................................................................	25	7	0.14	1	0	0	0	0	0	0	1	0	0	0
..............................TTGGGCATGTCGTCCTCATTTT.............................................................................................................................................	22	7	0.14	1	0	0	0	0	0	0	0	0	1	0
..........................................................................................................................................................................CGGACGGGGAAGCAGCACGGC..	21	12	0.08	1	0	0	0	0	0	0	0	0	1	0
.........................................................................................................................................................................GCGGACGGGGAAGCAGCACGGCAT	24	12	0.08	1	0	0	0	0	0	0	0	0	1	0
.........................................................................................................................................................................GCGGACGGGGAAGCAGCACGGCA.	23	12	0.08	1	0	0	0	0	0	0	0	0	1	0
........................................................................................................................TGATCCGAATCTGGGGCAGAAGCTG................................................	25	16	0.06	1	0	0	0	0	0	0	0	0	1	0
...........................................................................................................................TCCGAATCTGGGGCAGAAGCTGCA..............................................	24	17	0.06	1	0	0	0	0	0	0	0	0	1	0
...........................................................................................................................TCCGAATCTGGGGCAGAAGCTG................................................	22	17	0.06	1	0	0	0	0	0	0	0	0	1	0

droAna3

CCGCAGATGAGCATTATCTGGGCCAGGCTCTTGGCGATATCCTCCACGTTGTCGTCGAACAGCTGATAGGGA-TCCAGCTTCCTTCTGCAATTG---------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M044 Female-body	M058 Embryo	V039 Embryo	V055 Head	V105 Male-body	V106 Head
No Reads

droBip1

CCGCAGATGAGCATTATCTGGGCCAGGCTCTTGGCGATGTCCTCCACGTTGTCGTCGAACAGCTGATAGGGA-TCCAGCTTCCTCCTGCAGTT----------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droFic1

C-------------------------------------------------------------------------------------------------------------------------------------------------ATCTGCGAGTGCAACATCGATCAGCTGACTGGCGAGCAGTTCGGCG-	Size	Hit Count	Total Norm	Total
No Reads

droBia1

C-------------------------------------------------------------------------------------------------------------------------------------------------ATCTGCGAGTGCAACATCGACCAGTTGACGGGCGAGCAGTTCGGCG-	Size	Hit Count	Total Norm	Total
No Reads

droSim2

CCGCAGATCAGCATTATCTGGGCCAGGCTCTTGGCTATGTCCTCCACATTATCGTCAAACAGTTGGTAGGGA-TCCAGCTTCCTCCTGCAGTTGCAGCA----------------------------------------------------------------------------------------------

Size

Hit Count

Total Norm

Total

M023
Head

M024
Male-body

M025
Embryo

M053
Female-body

O001
Testis

O002
Head

SRR553485
Ovary

SRR553486
Ovary

SRR553487
Ovary

SRR553488
Ovary

SRR618934
Ovary

V044
Embryo

SRR902008
Ovary

SRR902009
Testis

SRR1275487
Male larvae

SRR1275485
Male prepupae

SRR1275483
Male prepupae

No Reads

droSec2

CCGCAGATCAGCATTATCTGGGCCAGGCTCTTGGCTATGTCCTCCACATTATCGTCAAACAGTTGGTAGGGA-TCCAGCTTCCTCCTGCAGTTGCAGCA----------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M054 Female-body	V113 Male-body	V114 Embryo	V115 Head
No Reads

-33.8	-33.5	-33.2	-33.0

dpe_298

scaffold_23:706912-707002 +

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads