dpe_280

CTCCACAAATAATGATTAAATGAAAATTAAGCAAACACTTCAAGTTTATTACCAGTCCAGATTTGTTAAACGTTACTTTCCTTAACTCGTATCACAGCTCTAGCCTGGCAATTATGCTTGAAAAATACATCTTTTTAGAAAATTTCGATATAAGCGCACAA *********************************...(((((((..(((.((((...(((.((((...(((...............)))...)))).)))...)))).)))...)))))))...**********************************	Read size	# Mismatch	Hit Count	Total Norm	Total	V111 male body	M042 female body	M021 embryo	V050 head	V057 head
.........................................................................................TATCACAGCTCTAGCCTGGCA...................................................	21	0	4	2.50	10	0	7	2	1	0
..................................................ACCAGTCCAGATTTGTTAAACGT........................................................................................	23	0	1	2.00	2	2	0	0	0	0
.........................................................................................TATCACAGCTCTAGCCTGGCAA..................................................	22	0	4	2.00	8	1	5	2	0	0
..................................................ACCAGTCCAGATTTGTTAAACGTTACTT...................................................................................	28	0	1	2.00	2	2	0	0	0	0
..................................................ACCAGTCCAGATTTGTTAAACG.........................................................................................	22	0	1	1.00	1	1	0	0	0	0
.........................................................................................TATTACAGCTCTAGCCTGG.....................................................	19	1	4	0.75	3	0	0	3	0	0
.........................................................................................TATCACAGCTCTAGCCTGG.....................................................	19	0	4	0.50	2	0	2	0	0	0
.........................................................................................TATTACAGCTCTAGCCTGGCAAAA................................................	24	3	4	0.25	1	0	0	1	0	0
.........................................................................................TATCACAGCTCTAGCCTAGCAA..................................................	22	1	4	0.25	1	0	0	1	0	0
.........................................................................................TATCACAGCTCTAGCCTGGCAT..................................................	22	1	4	0.25	1	0	1	0	0	0
.........................................................................................TATTACAGCTCTAGCCTG......................................................	18	1	4	0.25	1	0	0	1	0	0
.........................................................................................TATCACAGGTCTAGCCTGGCAA..................................................	22	1	4	0.25	1	0	1	0	0	0
.........................................................................................TATCACAGCTCTAGCCTGGCG...................................................	21	1	4	0.25	1	0	0	1	0	0
.........................................................................................TATCACAGCTCTAGCCTGGTT...................................................	21	2	4	0.25	1	0	1	0	0	0
.........................................................................................TATCACAGCTCTAGCCTGGC....................................................	20	0	4	0.25	1	0	1	0	0	0
...........................................................................................................................................AAATTTCGGTATAGGCGCGC..	20	3	8	0.13	1	0	0	0	0	1

GAGGTGTTTATTACTAATTTACTTTTAATTCGTTTGTGAAGTTCAAATAATGGTCAGGTCTAAACAATTTGCAATGAAAGGAATTGAGCATAGTGTCGAGATCGGACCGTTAATACGAACTTTTTATGTAGAAAAATCTTTTAAAGCTATATTCGCGTGTT **********************************...(((((((..(((.((((...(((.((((...(((...............)))...)))).)))...)))).)))...)))))))...*********************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M042 female body	V050 head
..............................................................................................GTAGTGATCGGACCGTTA.................................................	18	2	7	0.14	1	1	0
..................................................................................................TGCTCGGACCGTTCATACG............................................	19	3	20	0.05	1	0	1

Species	Coordinate	ID	Alignment
droPer2	scaffold_10:2580976-2581136 +	dpe_280	CTCCACAAATAATGATTAAATGAAAATTAAGCAAACACTTCAAGTTTATTACCAGTCCAGATTTGTTAAACGTTACTTTCCTTAACTCGTATCACAGCTCTAGCCTGGCAATTATGCTTGAAAAATACATCTTTTTAGAAAATTTCGATATAAGCGCACAA
dp5	4_group4:3552117-3552277 +	dps_41	CTCCACAAAGAATGATTAAATGAAAATTAAGCAAACACTTCAAGTTTATTACCAGGCCAGATTTGTAAAACGTTACTTTCCTTAACTCGTATTACAGCTCTAGCCTGGCAATTTTCCTTGAACAATACCTCTTTTTAGAAAATTTCGAGATAAGCGCACAA

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droPer2

CTCCACAAATAATGATTAAATGAAAATTAAGCAAACACTTCAAGTTTATTACCAGTCCAGATTTGTTAAACGTTACTTTCCTTAACTCGTATCACAGCTCTAGCCTGGCAATTATGCTTGAAAAATACATCTTTTTAGAAAATTTCGATATAAGCGCACAA	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
.........................................................................................TATCACAGCTCTAGCCTGGCA...................................................	21	4	2.50	10	2	7	0	1	0	0
..................................................ACCAGTCCAGATTTGTTAAACGT........................................................................................	23	1	2.00	2	0	0	0	0	0	2
.........................................................................................TATCACAGCTCTAGCCTGGCAA..................................................	22	4	2.00	8	2	5	0	0	0	1
..................................................ACCAGTCCAGATTTGTTAAACGTTACTT...................................................................................	28	1	2.00	2	0	0	0	0	0	2
..................................................ACCAGTCCAGATTTGTTAAACG.........................................................................................	22	1	1.00	1	0	0	0	0	0	1
.........................................................................................TATCACAGCTCTAGCCTGG.....................................................	19	4	0.50	2	0	2	0	0	0	0
.........................................................................................TATCACAGCTCTAGCCTGGCAT..................................................	22	4	0.25	1	0	1	0	0	0	0
.........................................................................................TATCACAGCTCTAGCCTGGCG...................................................	21	4	0.25	1	1	0	0	0	0	0
.........................................................................................TATCACAGCTCTAGCCTGGTT...................................................	21	4	0.25	1	0	1	0	0	0	0
.........................................................................................TATCACAGCTCTAGCCTGGC....................................................	20	4	0.25	1	0	1	0	0	0	0

dp5

CTCCACAAAGAATGATTAAATGAAAATTAAGCAAACACTTCAAGTTTATTACCAGGCCAGATTTGTAAAACGTTACTTTCCTTAACTCGTATTACAGCTCTAGCCTGGCAATTTTCCTTGAACAATACCTCTTTTTAGAAAATTTCGAGATAAGCGCACAA	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
.........................................................................................TATTACAGCTCTAGCCTGGCAA..................................................	22	1	1478.00	1478	16	210	0	40	0	14	13	0	1185	0
.........................................................................................TATTACAGCTCTAGCCTGGCA...................................................	21	1	786.00	786	26	121	1	14	1	2	7	0	614	0
.........................................................................................TATTACAGCTCTAGCCTGG.....................................................	19	1	164.00	164	6	64	0	5	0	1	2	0	86	0
..................................................ACCAGGCCAGATTTGTAAAACG.........................................................................................	22	1	80.00	80	2	1	0	0	0	0	8	0	69	0
.........................................................................................TATTACAGCTCTAGCCTGGCAAA.................................................	23	1	80.00	80	0	3	0	0	0	0	1	0	76	0
.........................................................................................TATTACAGCTCTAGCCTGGCAT..................................................	22	1	79.00	79	0	7	0	2	0	1	4	0	65	0
.........................................................................................TATTACAGCTCTAGCCTGGC....................................................	20	1	68.00	68	0	9	0	4	0	1	1	0	53	0
..................................................ACCAGGCCAGATTTGTAAAAC..........................................................................................	21	1	65.00	65	0	0	0	0	0	0	1	0	63	1
..........................................................................................ATTACAGCTCTAGCCTGGCAA..................................................	21	1	31.00	31	0	19	0	0	0	0	0	0	12	0
.........................................................................................TATTACAGCTCTAGCCTGGCAAAA................................................	24	1	16.00	16	0	0	0	0	0	0	0	0	16	0
.........................................................................................TATTACAGCTCTAGCCTGGCC...................................................	21	1	12.00	12	0	2	0	0	0	0	0	0	10	0
.........................................................................................TATTACAGCTCTAGCCTG......................................................	18	1	12.00	12	10	0	0	1	0	0	0	0	1	0
..........................................................................................ATTACAGCTCTAGCCTGGCA...................................................	20	1	11.00	11	0	9	0	0	0	0	0	0	2	0
..................................................ACCAGGCCAGATTTGTAAAACGT........................................................................................	23	1	10.00	10	0	0	0	0	0	0	8	0	2	0
.........................................................................................TATTACAGCTCTAGCCTGGCT...................................................	21	1	10.00	10	0	2	0	2	0	0	1	0	5	0
.........................................................................................TATTACAGCTCTAGCCTGGCAAT.................................................	23	1	10.00	10	0	2	0	0	0	0	0	0	8	0
.........................................................................................TATTACAGCTCTAGCCTGGT....................................................	20	1	9.00	9	0	0	0	0	0	2	0	0	7	0
..................................................ACCAGGCCAGATTTGTAAA............................................................................................	19	1	8.00	8	0	0	0	0	0	1	2	0	5	0
.........................................................................................TATTACAGCTCTAGCCTGGCAATT................................................	24	1	6.00	6	0	0	0	0	0	0	0	0	6	0
..........................................................................................ATTACAGCTCTAGCCTGG.....................................................	18	1	5.00	5	0	5	0	0	0	0	0	0	0	0
.........................................................................................TATTACAGCTCTAGCCTGGCAAAAA...............................................	25	2	3.50	7	0	0	0	0	0	0	0	1	6	0
.........................................................................................TATTACAGCTCTAGCCTGGCCC..................................................	22	1	3.00	3	1	0	0	0	0	0	0	0	2	0
..................................................ACCAGGCCAGATTTGTAAAA...........................................................................................	20	1	3.00	3	0	1	0	0	0	0	0	0	2	0
.........................................................................................TATTACAGCTCTAGCCTGGA....................................................	20	1	3.00	3	0	2	0	1	0	0	0	0	0	0
..................................................ACCAGGCCAGATTTGTAAAACT.........................................................................................	22	1	3.00	3	1	0	0	0	0	0	0	0	2	0
.........................................................................................TATTACAGCTCTAGCCTGGCATC.................................................	23	1	3.00	3	0	0	0	0	0	0	0	0	3	0
.........................................................................................TATTACAGCTCTAGCCTGT.....................................................	19	1	3.00	3	1	0	0	0	0	2	0	0	0	0
.........................................................................................TATTACAGCTCTAGCCTGGTC...................................................	21	1	3.00	3	0	0	0	0	0	0	0	0	3	0
........................................................................................GTATTACAGCTCTAGCCTGGCA...................................................	22	1	2.00	2	0	0	0	0	0	0	0	0	2	0
..................................................ACCAGGCCAGATTTGTAA.............................................................................................	18	1	2.00	2	0	0	0	0	0	0	0	0	2	0
.................................................TACCAGGCCAGATTTGTAAAAC..........................................................................................	22	1	2.00	2	0	0	0	0	0	0	0	0	2	0
.........................................................................................TATTACAGCTCTAGCCTGGCAC..................................................	22	1	2.00	2	0	1	0	0	0	0	0	0	1	0
.............................................................................................ACAGCTCTAGCCTGGCAA..................................................	18	2	2.00	4	0	1	0	2	0	0	0	0	1	0
.........................................................................................TATTACAGCTCTAGCCTGGCTT..................................................	22	1	2.00	2	0	0	0	0	0	0	0	0	2	0
.........................................................................................TATTACAGCTCTAGCCTGGCAGA.................................................	23	1	2.00	2	0	0	0	0	0	0	0	0	2	0
.........................................................................................TATTACAGCTCTAGCCTGGCATAA................................................	24	1	2.00	2	0	0	0	0	0	0	0	0	2	0
.........................................................................................TATTACAGCTCTAGCCTGGCAG..................................................	22	1	2.00	2	0	1	0	0	0	0	0	0	1	0
.........................................................................................TATTACAGCTCTAGCCTGGCAATC................................................	24	1	2.00	2	1	0	0	0	0	0	0	0	1	0
.........................................................................................TATTACAGCTCTAGCCTGGCG...................................................	21	1	2.00	2	1	0	0	0	0	0	0	0	1	0
..........................................................................................ATTACAGCTCTAGCCTGGCAAA.................................................	22	1	1.00	1	0	1	0	0	0	0	0	0	0	0
..................................................ACCAGGCCAGATTTGTAAAACGTC.......................................................................................	24	1	1.00	1	0	0	0	0	0	0	1	0	0	0
..................................................ACCAGGCCAGATTTGTAAAACGTTA......................................................................................	25	1	1.00	1	0	0	0	0	0	0	0	0	1	0
.................................................................................TTAACTCGTATTACAGCTCTAGCCTGGC....................................................	28	1	1.00	1	0	0	0	0	0	0	0	0	1	0
.........................................................................................TATTACAGCTCTAGCCTTT.....................................................	19	2	1.00	2	0	0	0	0	0	0	0	0	2	0
.........................................................................................TATTACAGCTCTAGCCTGGCAATTTGG.............................................	27	1	1.00	1	0	0	0	0	0	0	0	0	1	0
..........................................................................................ATTACAGCTCTAGCCTGGCAAT.................................................	22	1	1.00	1	0	0	0	0	0	0	0	0	1	0
.........................................................................................TATTACAGCTCTAGCCTT......................................................	18	1	1.00	1	1	0	0	0	0	0	0	0	0	0
...........................................................................................TTACAGCTCTAGCCTGGCAA..................................................	20	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.........................................................................................TATTACAGCTCTAGCCTGGCAAAG................................................	24	1	1.00	1	0	0	0	0	0	0	0	0	1	0
.........................................................................................TATTACAGCTCTAGCCTGGCAAC.................................................	23	1	1.00	1	0	0	0	0	0	0	0	0	1	0
..................................................ACCAGGCCAGATTTGTAAAACGC........................................................................................	23	1	1.00	1	0	0	0	0	0	0	0	0	1	0
.........................................................................................TATTACAGCTCTAGCCTGGCATA.................................................	23	1	1.00	1	1	0	0	0	0	0	0	0	0	0
............................................................................................TACAGCTCTAGCCTGGCAAA.................................................	20	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.........................................................................................TATTACAGCTCTAGCCTGGCAACC................................................	24	1	1.00	1	0	1	0	0	0	0	0	0	0	0
..................................................ACCAGGCCAGATTTGTAAAAA..........................................................................................	21	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.........................................................................................TATTACAGCTCTAGCCTGGTT...................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	1	0
.........................................................................TACTTTCCTTAACTCGTATTACAGC...............................................................	25	1	1.00	1	0	0	0	0	0	0	0	0	1	0
.........................................................................................TATTACAGCTCTAGCCTGGTTC..................................................	22	1	1.00	1	0	1	0	0	0	0	0	0	0	0
...........................................................................................TTACAGCTCTAGCCTGGCA...................................................	19	1	1.00	1	0	0	0	0	0	0	0	0	1	0
.........................................................................................TATTACAGCTCTAGCCTGGCATCC................................................	24	1	1.00	1	1	0	0	0	0	0	0	0	0	0
..........................................................................................ATTACAGCTCTAGCCTGGCAC..................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	1	0
.......................................................................................CGTATTACAGCTCTAGCCTGGC....................................................	22	1	1.00	1	0	0	0	0	0	0	0	0	1	0
.........................................................................................TATTACAGCTCTAGCCTGGCCCC.................................................	23	1	1.00	1	0	0	0	0	0	0	0	0	1	0
..............................................................................................CAGCTCTAGCCTGGCAAC.................................................	18	3	0.33	1	0	0	0	1	0	0	0	0	0	0

dpe_280

scaffold_10:2581026-2581086 +

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-16.2	-16.0	-15.8