ID:

dpe_2146

 Coordinate:

scaffold_9:966659-966809 +

 Confidence:

Candidate

 Type:

Unknown

 [View on UCSC Genome Browser {Cornell Mirror}]
Legend: mature star mismatch in alignment mismatch in read

Predicted structure

-29.1 -29.1 -29.1

Flybase annnotation

exon [dper_GLEANR_7539:4]; CDS [Dper\GL25167-cds]; intron [Dper\GL25167-in]

No Repeatable elements found

Sense Strand Reads

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------##################################################
GCCTTTGCCTATACATTTCCTATTATATTTCAGTCTCAAGCAGACGTCGACACGTTCACAGTTAAATTTCTCAGTAATTCGTTATGATTTCTGGGAAAACACAGTCAACACAAACTCGCAAAACAGAATTTTCGAGGTAAGTTGTCTGAAAATCTCTCCTCTAAGCCACCTTTGATTCTTGTGTCAATTTTTTTGATACAGAAACACCACCGCCAACACAACTGGAGCCCTTCTACTGGATTCGATACAGC
**************************************************................((((((((.((((......)))).))))))))...(((.((((.....((((.((((.....))))))))....)))).)))..............................(((((((((.....)))))))))************************************************** 		Read size # Mismatch Hit Count Total Norm Total V111
male body		 V057
head
 M021
embryo
....................................................................................TGATTTCTGGGAAAACACAGTC................................................................................................................................................. 22 0 1 1.00 1 1 0 0
......................................................................................................................................AGGTAAGTTGTCGGAAACTGT................................................................................................ 21 3 3 0.33 1 0 1 0
.................................................................................................................................................................................CTTGAGTCACTTTTTTTG........................................................ 18 2 20 0.25 5 0 0 5
..................................................................................................................................TTCGGGGTAGGTTGGCTGA...................................................................................................... 19 3 12 0.08 1 0 1 0
...............................................................................................................................................................TCTAAGCCACCTTTGATGAG........................................................................ 20 3 15 0.07 1 0 1 0
........................................................................................................................................................................CCTTTGATTCATGAGTCGA................................................................ 19 3 20 0.05 1 0 1 0

Anti-sense strand reads

CGGAAACGGATATGTAAAGGATAATATAAAGTCAGAGTTCGTCTGCAGCTGTGCAAGTGTCAATTTAAAGAGTCATTAAGCAATACTAAAGACCCTTTTGTGTCAGTTGTGTTTGAGCGTTTTGTCTTAAAAGCTCCATTCAACAGACTTTTAGAGAGGAGATTCGGTGGAAACTAAGAACACAGTTAAAAAAACTATGTCTTTGTGGTGGCGGTTGTGTTGACCTCGGGAAGATGACCTAAGCTATGTCG
**************************************************................((((((((.((((......)))).))))))))...(((.((((.....((((.((((.....))))))))....)))).)))..............................(((((((((.....)))))))))************************************************** 		Read size # Mismatch Hit Count Total Norm Total V057
head
 M042
female body
...........................................................................................................................................................GAGGAGATTCGGCCGACACTAA.......................................................................... 22 3 3 0.33 1 1 0
............................................................................................CCCTTTTGGGTCAGGTGGG............................................................................................................................................ 19 3 20 0.20 4 0 4
.......................................................................................................................................................................................................................TGTGTTGACCTCCGGAAC.................. 18 2 8 0.13 1 1 0
......................................................................................................................................................................................................................TTGTGTTGAGCTAGGGAAT.................. 19 3 20 0.05 1 1 0

Re-alignment of all predicted orthologs

Species Coordinate ID Alignment
droPer2 scaffold_9:966609-966859 + dpe_2146 GCCTTTGCCTATACATTTCCTATTATATTTCAGTCTCAAGCAGACGTCGACACGTTCACAGTTAAATTTCTCAGTAATTCGTTATGATTTCTGGGAAAACACAGTCAACACAAACTCGCAAAACAGAATTTTCGAGGTAAGTTGTCTGAAAATCTCTCCTCTAAGCCACCTTTGATTCTTGTGTCAATTTTTTTGATACAGAAACACCACCGCCAACACAACTGGAGCCCTTCTACTGGATTCGATACAGC
dp5 XR_group6:2670483-2670733 + GCCTTTGCCTGTAAATTTCCTATTATATTTCAGTCTCAAGCAGACGTCGACACGTTCACACCTAAATTTCTCAGTAATTCGTTATGATTTCTGGGAAAACACAGTCAACACAAACTCGCAAAACAGAATTTTCGAGGTAAGTTGTCTGAAAATCTCTCCTCTAAGTCACCTTTGATTCTTGTGTCAATTTTTTTGATACAGAAACACCACCCCCAACACAACTGGAGCCCTTCTACTGGATTCGATACAGC

Re-alignment of all predicted orthologs with sRNA reads

Species Read alignment
droPer2
GCCTTTGCCTATACATTTCCTATTATATTTCAGTCTCAAGCAGACGTCGACACGTTCACAGTTAAATTTCTCAGTAATTCGTTATGATTTCTGGGAAAACACAGTCAACACAAACTCGCAAAACAGAATTTTCGAGGTAAGTTGTCTGAAAATCTCTCCTCTAAGCCACCTTTGATTCTTGTGTCAATTTTTTTGATACAGAAACACCACCGCCAACACAACTGGAGCCCTTCTACTGGATTCGATACAGCSizeHit CountTotal NormTotalM021
Embryo		M042
Female-body		V042
Embryo		V050
Head		V057
Head		V111
Male-body
....................................................................................TGATTTCTGGGAAAACACAGTC.................................................................................................................................................2211.001000001
...............................................................................................................................................................TCTAAGCCACCTTTGATGAG........................................................................20150.071000010
dp5
GCCTTTGCCTGTAAATTTCCTATTATATTTCAGTCTCAAGCAGACGTCGACACGTTCACACCTAAATTTCTCAGTAATTCGTTATGATTTCTGGGAAAACACAGTCAACACAAACTCGCAAAACAGAATTTTCGAGGTAAGTTGTCTGAAAATCTCTCCTCTAAGTCACCTTTGATTCTTGTGTCAATTTTTTTGATACAGAAACACCACCCCCAACACAACTGGAGCCCTTCTACTGGATTCGATACAGCSizeHit CountTotal NormTotalM022
Male-body		M040
Female-body		M059
Embryo		M062
Head		V043
Embryo		V051
Head		V112
Male-body		SRR902010
Ovary		SRR902011
Testis		SRR902012
CNS imaginal disc
...........................................................................AATTCGTTATGATTTCTGGGA...........................................................................................................................................................2111.0010000000010
........................................................................................................................................................................................................................................CTACTGGATTCGATACAGC1911.0010100000000

Generated: 06/08/2015 at 05:15 PM