| G-AAT-----------------------------------------------------------------------------------------------------------------------------ATATATACTTTATATGGTCGGAAAGGCTTCCTTCTACCTGTTACATATTT-------TTCAACAAATCTAATAAGCCCA--TTCG-GTC------TATGAGTAACGGGTATA--AAAATAAATA--------AAA---TAATAA--------A---TA--ATACTAAAAAATGT---------------------------------GAT-----GTG-TCGGGGATGGGA--ATTTTGGGCGCTTGAATCAGGAAAGAACCCAACTT | Size | Hit Count | Total Norm | Total | M023 Head | M024 Male-body | M025 Embryo | M053 Female-body | O001 Testis | O002 Head | SRR553485 Ovary | SRR553486 Ovary | SRR553487 Ovary | SRR553488 Ovary | SRR618934 Ovary | V044 Embryo | SRR902008 Ovary | SRR902009 Testis | SRR1275487 Male larvae | SRR1275485 Male prepupae | SRR1275483 Male prepupae |
| ....................................................................................................................................ATATACTTTATATGGTCGGAAAGGC................................................................................................................................................................................................................................................. | 25 | 5 | 15.80 | 79 | 0 | 0 | 0 | 0 | 0 | 0 | 69 | 2 | 5 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
| ....................................................................................................................................ATATACTTTATATGGTCGGAAAGG.................................................................................................................................................................................................................................................. | 24 | 5 | 2.40 | 12 | 0 | 0 | 0 | 0 | 0 | 0 | 9 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
| ...AT-----------------------------------------------------------------------------------------------------------------------------ATATATACTTTATATGGTCGGAA..................................................................................................................................................................................................................................................... | 25 | 20 | 1.70 | 34 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 34 | 0 | 0 | 0 |
| ...................................................................................................................................................................................................................................................................................................TACTAAAAAATGT---------------------------------GAT-----GTG-TCGGGGA.......................................... | 26 | 3 | 1.67 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...AT-----------------------------------------------------------------------------------------------------------------------------ATATATACTTTATATGGTCGGAAA.................................................................................................................................................................................................................................................... | 26 | 20 | 1.20 | 24 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 24 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ....................................................................................................................................................................................................................TCG-GTC------TATGAGTAACGGGTATA--AAAA...................................................................................................................................................... | 27 | 3 | 1.00 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .....................................................................................................................................................................................................................CG-GTC------TATGAGTAACGGGTATA--AAAA...................................................................................................................................................... | 26 | 3 | 0.67 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...................................................................................................................................................................................T-------TTCAACAAATCTAATAAGCCCA--TTCG-G..................................................................................................................................................................................... | 28 | 3 | 0.67 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ......................................................................................................................................ATACTTTATATGGTCGGAAAGGCTTCC............................................................................................................................................................................................................................................. | 27 | 4 | 0.50 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...AT-----------------------------------------------------------------------------------------------------------------------------ATATATACTTTATATGGTCGGAC..................................................................................................................................................................................................................................................... | 25 | 20 | 0.45 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 9 | 0 | 0 | 0 | 0 |
| .....................................................................................................................................TATACTTTATATGGTCGGAAAGGCT................................................................................................................................................................................................................................................ | 25 | 5 | 0.40 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .............................................................................................................................................................................................................................................................................................................................................................................TTTGGGCGCTTGAATCAGGAAAGAACC...... | 27 | 3 | 0.33 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...........................................................................................................................................................................................................................................................................................................................................................................ATTTTGGGCGCTTGAATCAGGAAAGA......... | 26 | 3 | 0.33 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ............................................................................................................................................................................................................................................................................TAATAA--------A---TA--ATACTAAAAAATGT---------------------------------GA........................................................... | 25 | 3 | 0.33 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .......................................................................................................................................................................................................................................................................................................................................................................................TGAATCAGGAAAGAACCCAACTT | 23 | 3 | 0.33 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..........................................................................................................................................................................................................................................................................................A---TA--ATACTAAAAAATGT---------------------------------GAT-----GTG-TCGGGG........................................... | 29 | 3 | 0.33 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ....................................................................................................................................................................................................TCTAATAAGCCCA--TTCG-GTC------TATGAG....................................................................................................................................................................... | 26 | 3 | 0.33 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ......................................................................................................................................................................................................................................................................................................................................................................................TTGAATCAGGAAAGAACCCAACTT | 24 | 3 | 0.33 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .........................................................................................................................................................................................................TAAGCCCA--TTCG-GTC------TATGAGTAACGG................................................................................................................................................................. | 27 | 3 | 0.33 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .......................................................................................................................................................................................................AATAAGCCCA--TTCG-GTC------TATGAG....................................................................................................................................................................... | 23 | 3 | 0.33 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...................................................................................................................................................................................................................................TGAGTAACGGGTATA--AAAATAAATA--------AAA---.................................................................................................................................. | 28 | 3 | 0.33 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..............................................................................................................................................................TCCTTCTACCTGTTACATATT........................................................................................................................................................................................................................... | 21 | 7 | 0.29 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .............................................................................................................................................ATATGGTCGGAAAGGCTTCCTTCTAC....................................................................................................................................................................................................................................... | 26 | 4 | 0.25 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...........................................................................................................................................TTATATGGTCGGAAAGGCTTCCTTC.......................................................................................................................................................................................................................................... | 25 | 4 | 0.25 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..........................................................................................................................................TTTATATGGTCGGAAAGGCTTCCTTC.......................................................................................................................................................................................................................................... | 26 | 4 | 0.25 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..............................................................................................................................................TATGGTCGGAAAGGCTTCCTTCTACC...................................................................................................................................................................................................................................... | 26 | 4 | 0.25 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ................................................................................................................................................TGGTCGGAAAGGCTTCCTTCTAC....................................................................................................................................................................................................................................... | 23 | 4 | 0.25 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
| ............................................................................................................................................TATATGGTCGGAAAGGCTTCCTTC.......................................................................................................................................................................................................................................... | 24 | 4 | 0.25 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...................................................................................................................................TATATACTTTATATGGTCGGAAAGGC................................................................................................................................................................................................................................................. | 26 | 5 | 0.20 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ....................................................................................................................................ATATACTTTATATGGTCGGAAAG................................................................................................................................................................................................................................................... | 23 | 5 | 0.20 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..............................................................................................................................................TATGGTCGGAAAGGCTTCCTT........................................................................................................................................................................................................................................... | 21 | 5 | 0.20 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| .....................................................................................................................................TATACTTTATATGGTCGGAAAGGC................................................................................................................................................................................................................................................. | 24 | 5 | 0.20 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..........................................................................................................................................TTTATATGGTCGGAAAGGCTTCCTT........................................................................................................................................................................................................................................... | 25 | 5 | 0.20 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...........................................................................................................................................TTATATGGTCGGAAAGGC................................................................................................................................................................................................................................................. | 18 | 6 | 0.17 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..............................................................................................................................................TATGGTCGGAAAGGCTTCCTTCTACT...................................................................................................................................................................................................................................... | 26 | 6 | 0.17 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..............................................................................................................................................................TCCTTCTACCTGTTACATATTT-------TT................................................................................................................................................................................................................. | 24 | 7 | 0.14 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
| ....T-----------------------------------------------------------------------------------------------------------------------------ATATATACTTTATATGGTCGGA...................................................................................................................................................................................................................................................... | 23 | 20 | 0.10 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
| ....................................................................................................................................ATATACTTTATATGGTCGGAAAGGA................................................................................................................................................................................................................................................. | 25 | 12 | 0.08 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...................................................................................................................................................................................................................................TGAGTAACGGGTATA--AAAAA..................................................................................................................................................... | 20 | 16 | 0.06 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| .........................................................................................................................................................................................................................TC------TATGAGTAACGGGTA.............................................................................................................................................................. | 17 | 17 | 0.06 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| .........................................................................................................................................................................................................................TC------TATGAGTAACGGG................................................................................................................................................................ | 15 | 18 | 0.06 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..................................................................................................................................ATATATACTTTATATGGTCGGAAAGA.................................................................................................................................................................................................................................................. | 26 | 18 | 0.06 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
| ..AAT-----------------------------------------------------------------------------------------------------------------------------ATATATACTTTATATGGTCGGA...................................................................................................................................................................................................................................................... | 25 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
| G-AAT-----------------------------------------------------------------------------------------------------------------------------ATATATACTTTATATGGTCGGC...................................................................................................................................................................................................................................................... | 26 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ......................................................................................................................................................................................................................................GTAACGGGTATA--AAAA...................................................................................................................................................... | 16 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...........................................................................................................................................TTATATGGTCGGAAA.................................................................................................................................................................................................................................................... | 15 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..AAT-----------------------------------------------------------------------------------------------------------------------------ATATATACTTTATATGGTCGGAAA.................................................................................................................................................................................................................................................... | 27 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |