ID:

dpe_176

 Coordinate:

scaffold_15:1690640-1690711 +

 Confidence:

candidate

 Class:

Canonical miRNA

 Genomic Locale:

intron

 [View on UCSC Genome Browser {Cornell Mirror}]
Legend: mature star mismatch in alignment mismatch in read

Predicted structure

-55.7 -55.5 -55.1

Flybase annnotation

intron [Dper\GL16589-in]

No Repeatable elements found

Sense Strand Reads

CTCGTCCTGCAAACTGAGACGGGGCGGGAGGCCCGTGGGTCCCTGGCCTGTGCCGCCTGTAGTCTGTAACCACAATCTGTTAAGGATTTTCCACACGTGGCGGCAGGCTACAGGTGCTGCAGGCCAGTGCCCCCGATGCTCTCGCCACCGCCACCGTCGACGCTCAATCTAG

***********************************.((((..((((((((((.((((((((((((((..(((((((((.....)))))........))))..)))))))))))))).)))))))))).)))).....*********************************** 		Read size # Mismatch Hit Count Total Norm Total M021

embryo

 M042

female body

 V057

head

 V042

embryo

 V050

head

 V111

male body
..................................................TGCCGCCTGTAGTCTGTAACC..................................................................................................... 21 0 1 50.00 50 31 9 5 1 3 1
..................................................TGCCGCCTGTAGTCTGTAACCA.................................................................................................... 22 0 1 36.00 36 20 9 3 2 2 0
..................................................TGCCGCCTGTAGTCTGTAACCAC................................................................................................... 23 0 1 6.00 6 3 2 1 0 0 0
..................................................TGCCGCCTGTAGTCTGTAAC...................................................................................................... 20 0 1 5.00 5 0 3 2 0 0 0
.................................................GTGCCGCCTGTAGTCTGTAACC..................................................................................................... 22 0 1 3.00 3 3 0 0 0 0 0
..................................................TGCCGCCTGTAGTCTGTAACCC.................................................................................................... 22 1 1 2.00 2 0 0 0 2 0 0
..................................................TGCCGCCTGTAGTCTGTAACCACA.................................................................................................. 24 0 1 2.00 2 2 0 0 0 0 0
...................................................GCCGCCTGTAGTCTGTAACCA.................................................................................................... 21 0 1 2.00 2 0 2 0 0 0 0
..................................................TGCCGCCTGTAGTCTGTCACCAC................................................................................................... 23 1 1 1.00 1 0 0 1 0 0 0
..................................................TGCCGCCTGTAGGCTGTAACC..................................................................................................... 21 1 1 1.00 1 1 0 0 0 0 0
..................................................TGCCGCCTATAGTCTGTAACCA.................................................................................................... 22 1 1 1.00 1 0 1 0 0 0 0
..................................................TGCCGCCTGTAGTCTTTAAC...................................................................................................... 20 1 1 1.00 1 0 0 0 0 0 1
..................................................AGCCGCCTGTAGTCTGCAACCA.................................................................................................... 22 2 1 1.00 1 0 0 1 0 0 0
..................................................TGCCGCCTGTAGACTGTAACCA.................................................................................................... 22 1 1 1.00 1 0 0 1 0 0 0
..................................................TGCCGCATGTGGGCTGTAACC..................................................................................................... 21 3 1 1.00 1 0 0 1 0 0 0
.................................................GTGCCGCCTGTAGTCTGTAACCAC................................................................................................... 24 0 1 1.00 1 1 0 0 0 0 0
...............................CCCGTGGGTCCCTGGCCTG.......................................................................................................................... 19 0 1 1.00 1 1 0 0 0 0 0
...............GGGTCGGGTCGGGAGGCCCGTG....................................................................................................................................... 22 3 2 0.50 1 0 0 1 0 0 0
..................................................TGCCGCCTGTAGACTGTAGCC..................................................................................................... 21 2 3 0.33 1 0 0 1 0 0 0
..................................................TGCCCCCTGTACTCTGAAACC..................................................................................................... 21 3 6 0.17 1 0 0 1 0 0 0
............................................................................................................CACAGGTGCTGCATGCCTGT............................................ 20 3 17 0.06 1 0 0 1 0 0 0

Anti-sense strand reads

GAGCAGGACGTTTGACTCTGCCCCGCCCTCCGGGCACCCAGGGACCGGACACGGCGGACATCAGACATTGGTGTTAGACAATTCCTAAAAGGTGTGCACCGCCGTCCGATGTCCACGACGTCCGGTCACGGGGGCTACGAGAGCGGTGGCGGTGGCAGCTGCGAGTTAGATC

***********************************.((((..((((((((((.((((((((((((((..(((((((((.....)))))........))))..)))))))))))))).)))))))))).)))).....*********************************** 		Read size # Mismatch Hit Count Total Norm Total M042

female body

 V057

head
...................................................CGTCGGCCATCAGACATTG...................................................................................................... 19 2 2 0.50 1 1 0
............................................CCGGACAAGGACGACATCAGAC.......................................................................................................... 22 3 3 0.33 1 0 1
........................................GGGACCAGACACGGTGGGC................................................................................................................. 19 3 20 0.25 5 5 0
........................................GGGACCAGACACGGCGGGCGT............................................................................................................... 21 3 5 0.20 1 1 0
.............................................CGGACGCGGTGGACACCAGA........................................................................................................... 20 3 11 0.09 1 0 1
.....................................CCGGGGACCGGACAAGGCGC................................................................................................................... 20 3 16 0.06 1 0 1

Re-alignment of all predicted orthologs

Species Coordinate ID Type Alignment
droPer2 scaffold_15:1690590-1690761 + dpe_176 candidate CTCGTCCTGCAAACTGAGACGGGGCGGGAGGCCCGTGGGTCCCTGGCCTGTGCCGCCTGTAGTCTGTAACCACAATCTGTTAAGGATTTTCCACACGTGGCGGCAGGCTACAGGTGCTGCAGGCCAGTGCCCCCGATGCTCTCGCCACCGCCACCGTCGACGCTCAATCTAG
dp5 XL_group3a:1196731-1196902 - dps_3582 CTCGTCCTGCAAACTGAGACGGGGCGGGAGGACCGTGGGTCCCTGGCCTGTGCCGGCTGTAGCCTGTAACCACAATCTGTTAAGGATTTTCCACACGTGGCGGCAGGCTACAGGCGCTGCAGGCCAGTGCCCCCGATGCTCTCGCCACCGCCACCGTCGACGCTCAATCTAG
droSec2 scaffold_15:683877-683908 - CTCTTG-----AACT--------------GGCCGGTGGGTCTTTGGCCAGT-------------------------------------------------------------------------------------------------------------------------

Re-alignment of all predicted orthologs with sRNA reads

Species Read alignment
droPer2
CTCGTCCTGCAAACTGAGACGGGGCGGGAGGCCCGTGGGTCCCTGGCCTGTGCCGCCTGTAGTCTGTAACCACAATCTGTTAAGGATTTTCCACACGTGGCGGCAGGCTACAGGTGCTGCAGGCCAGTGCCCCCGATGCTCTCGCCACCGCCACCGTCGACGCTCAATCTAGSizeHit CountTotal NormTotalM021

Embryo		M042

Female-body		V042

Embryo		V050

Head		V057

Head		V111

Male-body
..................................................TGCCGCCTGTAGTCTGTAACC.....................................................................................................21150.00503191351
..................................................TGCCGCCTGTAGTCTGTAACCA....................................................................................................22136.00362092230
..................................................TGCCGCCTGTAGTCTGTAACCAC...................................................................................................2316.006320010
..................................................TGCCGCCTGTAGTCTGTAAC......................................................................................................2015.005030020
.................................................GTGCCGCCTGTAGTCTGTAACC.....................................................................................................2213.003300000
..................................................TGCCGCCTGTAGTCTGTAACCC....................................................................................................2212.002002000
..................................................TGCCGCCTGTAGTCTGTAACCACA..................................................................................................2412.002200000
...................................................GCCGCCTGTAGTCTGTAACCA....................................................................................................2112.002020000
.................................................GTGCCGCCTGTAGTCTGTAACCAC...................................................................................................2411.001100000
...............................CCCGTGGGTCCCTGGCCTG..........................................................................................................................1911.001100000
dp5
CTCGTCCTGCAAACTGAGACGGGGCGGGAGGACCGTGGGTCCCTGGCCTGTGCCGGCTGTAGCCTGTAACCACAATCTGTTAAGGATTTTCCACACGTGGCGGCAGGCTACAGGCGCTGCAGGCCAGTGCCCCCGATGCTCTCGCCACCGCCACCGTCGACGCTCAATCTAGSizeHit CountTotal NormTotalM022

Male-body		M040

Female-body		M059

Embryo		M062

Head		V043

Embryo		V051

Head		V112

Male-body		SRR902010

Ovary		SRR902011

Testis		SRR902012

CNS imaginal disc
..................................................TGCCGGCTGTAGCCTGTAACC.....................................................................................................2112.0020200000000
.........................................................................................................................GGCCAGTGCCCCCGATGCTCTCGCCAC........................2711.0010100000000
....................................................................................................CGGCAGGCTACAGGCGCTGCA...................................................2111.0010100000000
....................................................................................................................CTGCAGGCCAGTGCCCCCGAT...................................2111.0010100000000
...................................................GCCGGCTGTAGCCTGTAACC.....................................................................................................2011.0010100000000
.................................................GTGCCGGCTGTAGCCTGTAACC.....................................................................................................2211.0010010000000
..................................................TGCCGGCTGTAGCCTGTAAC......................................................................................................2011.0010100000000
......................................GTCCCTGGCCTGTGCCGGCTG.................................................................................................................2111.0010100000000
droSec2
CTCTTG-----AACT--------------GGCCGGTGGGTCTTTGGCCAGT-------------------------------------------------------------------------------------------------------------------------SizeHit CountTotal NormTotalM054

Female-body		V113

Male-body		V114

Embryo		V115

Head
No Reads

Generated: 10/20/2015 at 06:50 PM