ID:

dpe_166

 Coordinate:

scaffold_26:563931-563985 +

 Confidence:

candidate

 Class:

Canonical miRNA

 Genomic Locale:

intergenic

 [View on UCSC Genome Browser {Cornell Mirror}]
Legend: mature star mismatch in alignment mismatch in read

Predicted structure

-37.9

Flybase annnotation

intergenic

No Repeatable elements found

Sense Strand Reads

TCCTTCCCAACCAAAGAGCCCAGGGAGGAGTTCTGTTTCTGGCGACTCTGCCAGGAGTCACCATGCAATGTGGAATGTGGAACATTTAATGGTGCCTCCTGAGAGAGGCGGCAGGACTGCCATCGGTTGTCTGAGCATTAATCGTCGCGCCTTAT

***********************************.(((((.((.((((..((((((.((((((..(((((..........)))))..)))))).))))))..)))).)).)))))...************************************ 		Read size # Mismatch Hit Count Total Norm Total V057

head

 M042

female body

 V050

head

 V042

embryo

 V111

male body		 M021

embryo
..................................................CCAGGAGTCACCATGCAATGTG................................................................................... 22 0 1 5.00 5 3 0 0 1 1 0
....................................................................................TTTAATGGTGCCTCCTGAGA................................................... 20 0 1 2.00 2 0 2 0 0 0 0
..CTTCCCACCCAAAGAGCGC...................................................................................................................................... 19 2 2 1.00 2 2 0 0 0 0 0
..................................................................................CATTTAATGGTGCCTCCTGAGA................................................... 22 0 1 1.00 1 0 0 1 0 0 0
....................................................................................TTTAATGGTGCCTCCTGAGAG.................................................. 21 0 1 1.00 1 0 1 0 0 0 0
..................................................CCAGGAGTCACCATGCAATGTGT.................................................................................. 23 1 1 1.00 1 0 1 0 0 0 0
..................................................CCAGGAGTCACCATACAAAGAG................................................................................... 22 3 1 1.00 1 0 0 1 0 0 0
.........................................................................................................AGGAGGCAGGACTGCTAACGG............................. 21 3 2 0.50 1 1 0 0 0 0 0
.....................TGGGAGGAGTTCTGATACTGG................................................................................................................. 21 3 4 0.25 1 1 0 0 0 0 0
............................................ACTCTGGCAGGAGGCACCA............................................................................................ 19 2 19 0.05 1 0 0 0 0 0 1
..........................................................CACCTCGCAATGTGGAATC.............................................................................. 19 3 20 0.05 1 1 0 0 0 0 0

Anti-sense strand reads

AGGAAGGGTTGGTTTCTCGGGTCCCTCCTCAAGACAAAGACCGCTGAGACGGTCCTCAGTGGTACGTTACACCTTACACCTTGTAAATTACCACGGAGGACTCTCTCCGCCGTCCTGACGGTAGCCAACAGACTCGTAATTAGCAGCGCGGAATA

************************************.(((((.((.((((..((((((.((((((..(((((..........)))))..)))))).))))))..)))).)).)))))...*********************************** 		Read size # Mismatch Hit Count Total Norm Total V050

head

 M042

female body

 V057

head

 V042

embryo

 M021

embryo

 V111

male body
.........................................................................................ACCACGGAGGACACTCCCCAC............................................. 21 3 6 5.50 33 23 10 0 0 0 0
.........................................................................................ACCACGGAGGACACTCCCC............................................... 19 2 5 5.40 27 14 11 1 0 1 0
.......................................ACCGCTAATACGGTCCTCAGT............................................................................................... 21 2 1 1.00 1 1 0 0 0 0 0
.........................................................................................ACCACGGAGGACACTCTC................................................ 18 1 1 1.00 1 0 0 0 1 0 0
.......................................ACCGCTCATACGGTCCTCAG................................................................................................ 20 2 1 1.00 1 1 0 0 0 0 0
.........................................................................................ACCACGGAGGACACTCCCCGC............................................. 21 2 1 1.00 1 0 1 0 0 0 0
.........................................................................................ACCACGGAGGACACTCCCCC.............................................. 20 3 13 0.77 10 0 10 0 0 0 0
......................................................................................TTTACCACGGAGGACACTC.................................................. 19 2 3 0.67 2 0 0 2 0 0 0
.........................................................................................ACCACGGAGGACACTCCCCCC............................................. 21 3 5 0.60 3 1 2 0 0 0 0
.......................................ACCGCTACGATGGTCCTCAGT............................................................................................... 21 3 2 0.50 1 0 0 1 0 0 0
.........................................................................................ACCACGGAGGACTCTCCCAAC............................................. 21 3 2 0.50 1 0 1 0 0 0 0
.......................................ACCGCTGGGACGGTCCTCAGC............................................................................................... 21 2 4 0.50 2 2 0 0 0 0 0
.......................................ACCGCTAGGACGGTCCTCAGT............................................................................................... 21 2 4 0.50 2 2 0 0 0 0 0
.........................................................................................ACCACGGAGGACACTCCCCA.............................................. 20 3 17 0.41 7 0 6 1 0 0 0
.........................................................................................ACCACGGAGGACACTCCCCGCT............................................ 22 3 3 0.33 1 1 0 0 0 0 0
.......................................ACCGCTAAAACGGTCCTC.................................................................................................. 18 2 3 0.33 1 0 0 1 0 0 0
.......................................ACCGCTAAGACGGTCCTCAGC............................................................................................... 21 2 4 0.25 1 1 0 0 0 0 0
.......................................ACCGCTGGGACGGGCCTCAGC............................................................................................... 21 3 4 0.25 1 1 0 0 0 0 0
.......................................ACCGCAGGGACGGTCCTCAGC............................................................................................... 21 3 5 0.20 1 1 0 0 0 0 0
.......................................ACCGCTAAGACGGTCCTCGGC............................................................................................... 21 3 5 0.20 1 1 0 0 0 0 0
......................................CACCGCTGGGACGGTCCTCAGC............................................................................................... 22 3 6 0.17 1 1 0 0 0 0 0
.......................................ACCGCTAGGACGGTCCTCTGT............................................................................................... 21 3 6 0.17 1 1 0 0 0 0 0
..........................................................................................CCACGGAGGACACTCCCC............................................... 18 2 7 0.14 1 0 1 0 0 0 0
.......................................ACCGATAGGACGGTCCTCAGT............................................................................................... 21 3 7 0.14 1 0 0 1 0 0 0
.......................................ACCGCTCTGACGGTCCTCTG................................................................................................ 20 3 7 0.14 1 1 0 0 0 0 0
.......................................ACCGCTGGGACGGTCCTCGGC............................................................................................... 21 3 7 0.14 1 1 0 0 0 0 0
.......................................ACCCCTGGGACGGTCCTCGG................................................................................................ 20 3 8 0.13 1 1 0 0 0 0 0
......................................................................................TTTTCCACGGAGGACACTC.................................................. 19 3 17 0.12 2 0 0 2 0 0 0
.......................................ACCGCTTGGACGGTCCTCGG................................................................................................ 20 3 11 0.09 1 1 0 0 0 0 0
.................................................................................TGTCAACTACCACGGAGGAG...................................................... 20 3 12 0.08 1 0 0 0 1 0 0
................TCGGGACCCTCTTGAAGAC........................................................................................................................ 19 3 20 0.05 1 0 0 0 0 0 1
.......................................ACCGCTCGGACTGTCCTCAG................................................................................................ 20 3 20 0.05 1 1 0 0 0 0 0
.......................................ACCGCTGGCACGGTCCTCAG................................................................................................ 20 2 20 0.05 1 0 0 1 0 0 0

Re-alignment of all predicted orthologs

Species Coordinate ID Type Alignment
droPer2 scaffold_26:563881-564035 + dpe_166 candidate TCCTTCCCAACCAAAGAGCCCAGGGAGGAGTTCTGTTTCTGGCGACTCTGCCAGGAGTCACCATGCAATGTGGAA---TGTGGA---------------------------------------------------------------------------ACATTTAATGGTGCCTCCTGAGAGAGGCGGCAGGACTGCCATCGGTTGTCTGAGCATTAATCGTCGCGCCTTAT
dp5 XL_group1a:108363-108595 - TCCTTCCCAACCAAAGAGCCCAGGGAGGAGTTCTGTTTCTGGCGACTCTGCCAGAAGTCACCATGCAATGTGGAACATTGTGCAAGCCAAGCTATCAAGTTTTACATCAGTTAACATTTAAATACAATAATTTATCTGACCTGGCTTGCCCAAGGTTCCACATTTAATGGTGCCTCCTGAGAGAGGCGGCAGGACTGCCATCGGTTGTCTGAGCATTCATCGTCGCGCCTTAA

Re-alignment of all predicted orthologs with sRNA reads

Species Read alignment
droPer2
TCCTTCCCAACCAAAGAGCCCAGGGAGGAGTTCTGTTTCTGGCGACTCTGCCAGGAGTCACCATGCAATGTGGAA---TGTGGA---------------------------------------------------------------------------ACATTTAATGGTGCCTCCTGAGAGAGGCGGCAGGACTGCCATCGGTTGTCTGAGCATTAATCGTCGCGCCTTATSizeHit CountTotal NormTotalM021

Embryo		M042

Female-body		V042

Embryo		V050

Head		V057

Head		V111

Male-body
..................................................CCAGGAGTCACCATGCAATGTG.................................................................................................................................................................2215.005001031
..................................................................................................................................................................TTTAATGGTGCCTCCTGAGA...................................................2012.002020000
................................................................................................................................................................CATTTAATGGTGCCTCCTGAGA...................................................2211.001000100
..................................................................................................................................................................TTTAATGGTGCCTCCTGAGAG..................................................2111.001010000
..................................................CCAGGAGTCACCATGCAATGTGT................................................................................................................................................................2311.001010000
dp5
TCCTTCCCAACCAAAGAGCCCAGGGAGGAGTTCTGTTTCTGGCGACTCTGCCAGAAGTCACCATGCAATGTGGAACATTGTGCAAGCCAAGCTATCAAGTTTTACATCAGTTAACATTTAAATACAATAATTTATCTGACCTGGCTTGCCCAAGGTTCCACATTTAATGGTGCCTCCTGAGAGAGGCGGCAGGACTGCCATCGGTTGTCTGAGCATTCATCGTCGCGCCTTAASizeHit CountTotal NormTotalM022

Male-body		M040

Female-body		M059

Embryo		M062

Head		V043

Embryo		V051

Head		V112

Male-body		SRR902010

Ovary		SRR902011

Testis		SRR902012

CNS imaginal disc
No Reads

Generated: 10/20/2015 at 06:52 PM