dpe_164

TAAGTAAAAGTAAACGATGGAAACTGCACGTGAAAGGATGTTTCCTCATGCTGCATTGGATTTACTGCCACCAGTGCCGCCTGCCCTGTTCCACACATGGCAGTGGATCTCATCAGCCTACCTGGGTCGTGTGGTACTTACCCGCTGCTTCAATTCAAATTCCGTTCTC *********************************.....((..((((.((((.((.(((((((((((((...(((..((.......))..)))...))))))))))))).)))))).....))))......))*********************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M021 embryo	V050 head	V111 male body	V057 head
..................................................CTGCATTGGATTTACTGC.....................................................................................................	18	0	2	11.00	22	22	0	0	0
..........................................................................................CCACACATGGCTGCGGGTCTCA.........................................................	22	3	1	1.00	1	0	1	0	0
........................TGCACGTGAAAGGATGTTTCC............................................................................................................................	21	0	1	1.00	1	0	0	1	0
..................................................CTGCATTGGATTCACTGC.....................................................................................................	18	1	2	0.50	1	1	0	0	0
.................................................................................................TGGCAGGGGATCTGATAAGCC...................................................	21	3	2	0.50	1	0	1	0	0
..................................................CTGCATTGGATTGACTGC.....................................................................................................	18	1	2	0.50	1	1	0	0	0
.............................................................................................CACAGGACAGTGGATCTC..........................................................	18	2	5	0.20	1	0	0	0	1

TAAGTAAAAGTAAACGATGGAAACTGCACGTGAAAGGATGTTTCCTCATGCTGCATTGGATTTACTGCCACCAGTGCCGCCTGCCCTGTTCCACACATGGCAGTGGATCTCATCAGCCTACCTGGGTCGTGTGGTACTTACCCGCTGCTTCAATTCAAATTCCGTTCTC
***********************************.....((..((((.((((.((.(((((((((((((...(((..((.......))..)))...))))))))))))).)))))).....))))......))***********************************

Read size

# Mismatch

Hit Count

Total Norm

Total

M021
embryo

V050
head

V111
male body

V057
head

..................................................CTGCATTGGATTTACTGC.....................................................................................................

11.00

..........................................................................................CCACACATGGCTGCGGGTCTCA.........................................................

1.00

........................TGCACGTGAAAGGATGTTTCC............................................................................................................................

1.00

..................................................CTGCATTGGATTCACTGC.....................................................................................................

0.50

.................................................................................................TGGCAGGGGATCTGATAAGCC...................................................

0.50

..................................................CTGCATTGGATTGACTGC.....................................................................................................

0.50

.............................................................................................CACAGGACAGTGGATCTC..........................................................

0.20

ATTCATTTTCATTTGCTACCTTTGACGTGCACTTTCCTACAAAGGAGTACGACGTAACCTAAATGACGGTGGTCACGGCGGACGGGACAAGGTGTGTACCGTCACCTAGAGTAGTCGGATGGACCCAGCACACCATGAATGGGCGACGAAGTTAAGTTTAAGGCAAGAG *********************************.....((..((((.((((.((.(((((((((((((...(((..((.......))..)))...))))))))))))).)))))).....))))......))*********************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M042 female body
..........................................................................................................................ACCCAGCCCGCCAGGAATGG...........................	20	3	5	0.20	1	1
......................................................................................................................................ATGGTTGGGCGACGAAGTT................	19	2	8	0.13	1	1
.....................................................................................................................................AATGGTTGGGCGACGAAGTT................	20	3	10	0.10	1	1

ATTCATTTTCATTTGCTACCTTTGACGTGCACTTTCCTACAAAGGAGTACGACGTAACCTAAATGACGGTGGTCACGGCGGACGGGACAAGGTGTGTACCGTCACCTAGAGTAGTCGGATGGACCCAGCACACCATGAATGGGCGACGAAGTTAAGTTTAAGGCAAGAG
***********************************.....((..((((.((((.((.(((((((((((((...(((..((.......))..)))...))))))))))))).)))))).....))))......))***********************************

Read size

# Mismatch

Hit Count

Total Norm

Total

M042
female body

..........................................................................................................................ACCCAGCCCGCCAGGAATGG...........................

0.20

......................................................................................................................................ATGGTTGGGCGACGAAGTT................

0.13

.....................................................................................................................................AATGGTTGGGCGACGAAGTT................

0.10

Re-alignment of all predicted orthologs

Species	Coordinate	ID	Alignment
droPer2	scaffold_17:922873-923041 +	dpe_164	TAAGTAAAAGTAAACGATGGAAACTGCACGTGAAAGGATGTTTCCTCATGCTGCATTGGATTTACTGCCACCAGTGCCGCCTGCCCTGTTCCACACATGGCAGTGGATCTCATCAGCCTACCTGGGTCGTGT------------------------------GGTACTTACCCGCTGCTTCAATTCAAATTCCGTTCTC
dp5	XL_group1e:3988021-3988210 +		TAAGTAAAGGTAAACGATGGAAACTGCACGTCAAAGGATGTTTCTTCATGCTGCATTGGATTTACTGCCACCAGTGCCCCCTGCCCTGTTCCGCAGATGGCAGTGGATCTCATCAGCCTACCTGTGTCGTGTGGATCGCAGTTATCGAACGGCTTACGGGTTGGTACTTACCCGCTGTTTCAATTCAAAT---------

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droPer2

TAAGTAAAAGTAAACGATGGAAACTGCACGTGAAAGGATGTTTCCTCATGCTGCATTGGATTTACTGCCACCAGTGCCGCCTGCCCTGTTCCACACATGGCAGTGGATCTCATCAGCCTACCTGGGTCGTGT------------------------------GGTACTTACCCGCTGCTTCAATTCAAATTCCGTTCTC	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
..................................................CTGCATTGGATTTACTGC...................................................................................................................................	18	2	11.00	22	22	0	0	0	0	0
........................TGCACGTGAAAGGATGTTTCC..........................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	1

dp5

TAAGTAAAGGTAAACGATGGAAACTGCACGTCAAAGGATGTTTCTTCATGCTGCATTGGATTTACTGCCACCAGTGCCCCCTGCCCTGTTCCGCAGATGGCAGTGGATCTCATCAGCCTACCTGTGTCGTGTGGATCGCAGTTATCGAACGGCTTACGGGTTGGTACTTACCCGCTGTTTCAATTCAAAT---------	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
......................................................................................TGTTCCGCAGATGGCAGTGGATC..........................................................................................	23	1	1.00	1	0	0	0	0	0	0	0	0	1	0

-29.3	-28.9	-28.9	-28.8

dpe_164

scaffold_17:922923-922991 +

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads