dpe_1569

--------------------------########################--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- AGCTGTGTGTGTGTACAGTCTCCAAGATGGATACTCCCAATCCGGATGGAGTCAAGCCCGGATAGGCACTATCCTTGCAAATCGCTCCTGTTTAGGACCACCAACAATTTATACAGAGAGATATGTATATATTTTCAAAATATAGTGTGTTAAATATAGTATAGCGCTACGACGGACCGCAAATACGCAAGATCTACCAGCAGGCGCACTCATGCCTACGAGGCCTCACAATTTTAATGTTAAATAAAAGC ************************************************(((..((..((((((...))))))..)).......((((....))))........(((.((((((........)))))).))).........((((((..((.......))..)))))).))).....((......)).............************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M021 embryo	V111 male body	M042 female body	V057 head
.........................GATGGATACTCCCAATCCGGATGGAGT.......................................................................................................................................................................................................	27	0	1	2.00	2	0	2	0	0
...........................................................................TGCAAATCCCTCCTGTTTAGGACCACC.....................................................................................................................................................	27	1	1	1.00	1	0	0	1	0
.......................................................................................................................................................................TACGACGGACCGCAAATACGCAAGA...........................................................	25	0	1	1.00	1	1	0	0	0
................................................................................................................................................................ATAGCGCTACGACGGACCG........................................................................	19	0	1	1.00	1	1	0	0	0
..................................................................................................................................................................................................TACCACCAGACGCACTCA.......................................	18	2	7	0.14	1	0	0	0	1

TCGACACACACACATGTCAGAGGTTCTACCTATGAGGGTTAGGCCTACCTCAGTTCGGGCCTATCCGTGATAGGAACGTTTAGCGAGGACAAATCCTGGTGGTTGTTAAATATGTCTCTCTATACATATATAAAAGTTTTATATCACACAATTTATATCATATCGCGATGCTGCCTGGCGTTTATGCGTTCTAGATGGTCGTCCGCGTGAGTACGGATGCTCCGGAGTGTTAAAATTACAATTTATTTTCG ************************************************(((..((..((((((...))))))..)).......((((....))))........(((.((((((........)))))).))).........((((((..((.......))..)))))).))).....((......)).............************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M042 female body	V111 male body
..................................................................GTGATAGGCCCTTTTAGCGA.....................................................................................................................................................................	20	3	8	0.13	1	1	0
...................................GGGTGAGGCCTACTTCAGA.....................................................................................................................................................................................................	19	3	14	0.07	1	1	0
..................................................................GTGATAGGCCCTTTTAGCG......................................................................................................................................................................	19	3	20	0.05	1	0	1

Species	Coordinate	ID	Alignment
droPer2	scaffold_4:1867864-1868114 -	dpe_1569	AGCTGTGTGTGTGTACAGTCTCCAAGATGGATACTCCCAATCCGGATGGAGTCAAGCCCGGATAGGCAC------TATCCTTGCAAATCGCTCCTGTTTAGGACCACCAACAATTTATACAGAGAGATATGTATATATTTTCAAAATATAGTGTGTTAAATATAGTATA--------------------------------------------GCGCTACGACGGACCGCAAATACGCAAGATCTACCAGCAGGCGCACTCATGCCTACGAGGCCTCACAATTTTAATGTTAAATAAAAGC
dp5	3:7956701-7956971 -		AGCTGTGTGTGTGTACAGTCTCCAAGATGGATACTCCCAATCCGGATGGAGTCAACCCCGGATAGGCACTTATCCTATCCTTGCAAATCGCTCCTGTTCAGGACCACCAACAATTTA------------------------------ATAGTGTGTTAAATATAGTATAGTATGTTAAAATATAAATATAAAATCGTGCAAGTTCCTCACAAAGCGCTACGACGGACCGCAAATACGCAAGATCTACCAGCAGGCGCACTCATGCCTACGAGGCCTCACAATTTTAATGTTAAATAAAAGT

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droPer2

AGCTGTGTGTGTGTACAGTCTCCAAGATGGATACTCCCAATCCGGATGGAGTCAAGCCCGGATAGGCAC------TATCCTTGCAAATCGCTCCTGTTTAGGACCACCAACAATTTATACAGAGAGATATGTATATATTTTCAAAATATAGTGTGTTAAATATAGTATA--------------------------------------------GCGCTACGACGGACCGCAAATACGCAAGATCTACCAGCAGGCGCACTCATGCCTACGAGGCCTCACAATTTTAATGTTAAATAAAAGC	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
.........................GATGGATACTCCCAATCCGGATGGAGT.........................................................................................................................................................................................................................................................	27	1	2.00	2	0	0	0	0	0	2
.........................................................................................................................................................................................................................TACGACGGACCGCAAATACGCAAGA...........................................................	25	1	1.00	1	1	0	0	0	0	0
......................................................................................................................................................................ATA--------------------------------------------GCGCTACGACGGACCG........................................................................	19	1	1.00	1	1	0	0	0	0	0

dp5

AGCTGTGTGTGTGTACAGTCTCCAAGATGGATACTCCCAATCCGGATGGAGTCAACCCCGGATAGGCACTTATCCTATCCTTGCAAATCGCTCCTGTTCAGGACCACCAACAATTTA------------------------------ATAGTGTGTTAAATATAGTATAGTATGTTAAAATATAAATATAAAATCGTGCAAGTTCCTCACAAAGCGCTACGACGGACCGCAAATACGCAAGATCTACCAGCAGGCGCACTCATGCCTACGAGGCCTCACAATTTTAATGTTAAATAAAAGT	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
.........................................................................................................................................................................................................................TACGACGGACCGCAAATACGCAAGATC.........................................................	27	1	18.00	18	0	2	0	0	0	0	0	1	15	0
.................................................................................................TCAGGACCACCAACAATTTA------------------------------ATAG......................................................................................................................................................	24	1	6.00	6	0	0	0	0	0	0	0	0	6	0
............................................................................................................................................................................TGTTAAAATATAAATATAAAATCGTG.......................................................................................................	26	1	5.00	5	0	0	0	0	0	0	0	0	5	0
.......TGTGTGTACAGTCTCCAAGATGG...............................................................................................................................................................................................................................................................................	23	1	5.00	5	0	0	0	0	0	0	0	0	5	0
..........................................................................................................................................................................................TATAAAATCGTGCAAGTTCCTCACAA.........................................................................................	26	1	5.00	5	0	0	0	0	0	0	1	0	4	0
....................................................................................................................................................................................................TGCAAGTTCCTCACAAAGCGCTACGA...............................................................................	26	1	4.00	4	0	0	0	0	0	0	0	0	4	0
...............................................................................................................................................................................TAAAATATAAATATAAAATCGTGCAAG...................................................................................................	27	1	4.00	4	0	0	0	0	0	0	0	0	4	0
...................................................................................................................TA------------------------------ATAGTGTGTTAAATATAGTATAG...................................................................................................................................	25	1	4.00	4	0	0	0	0	0	0	0	0	4	0
............................................................................................................................................................................................TAAAATCGTGCAAGTTCCTCACAAAGC......................................................................................	27	1	3.00	3	0	0	0	0	0	0	0	0	3	0
.......................................................................................................................................................TGTGTTAAATATAGTATAGTATGT..............................................................................................................................	24	1	3.00	3	0	0	0	0	0	0	0	0	3	0
........................................TCCGGATGGAGTCAACCCCGGATAGG...........................................................................................................................................................................................................................................	26	1	3.00	3	0	0	0	0	0	0	0	0	3	0
......................................................................................................................................................................................TAAATATAAAATCGTGCAAGTTCCTCAC...........................................................................................	28	1	3.00	3	0	0	0	0	0	0	1	0	2	0
...........................................................................TATCCTTGCAAATCGCTCCTGTT...........................................................................................................................................................................................................	23	1	2.00	2	0	0	0	0	0	0	0	0	2	0
....................................................................................................................................................................................................................AGCGCTACGACGGACCGCAAATACGC...............................................................	26	1	2.00	2	0	0	0	0	0	0	0	0	2	0
...............................................................................................................................................................................TAAAATATAAATATAAAATCGTGCAAGT..................................................................................................	28	1	2.00	2	0	0	0	0	0	0	0	0	2	0
..................................................................................GCAAATCGCTCCTGTTCAGGACCACCAA...............................................................................................................................................................................................	28	1	2.00	2	0	0	0	0	0	0	0	0	2	0
............................................................................................................................................................TAAATATAGTATAGTATGTTAAAATA.......................................................................................................................	26	1	2.00	2	0	0	0	0	0	0	0	0	2	0
..............................................................TAGGCACTTATCCTATCCTTGCAAATCGC..................................................................................................................................................................................................................	29	1	2.00	2	0	0	0	0	0	0	0	0	2	0
..................................................................................................................................................................TAGTATAGTATGTTAAAATATAAATA.................................................................................................................	26	1	2.00	2	0	0	0	0	0	0	0	0	2	0
..................................................................................................................................................................TAGTATAGTATGTTAAAATATAAATAT................................................................................................................	27	1	2.00	2	0	0	0	0	0	0	0	0	2	0
..................................................................................................................................................................TAGTATAGTATGTTAAAATATAAA...................................................................................................................	24	1	1.00	1	0	0	0	0	0	0	0	0	1	0
........GTGTGTACAGTCTCCAAGATGGATAC...........................................................................................................................................................................................................................................................................	26	1	1.00	1	0	0	0	0	0	0	0	0	1	0
.................................................................................................TCAGGACCACCAACAATTTA------------------------------ATAGTGT...................................................................................................................................................	27	1	1.00	1	0	0	0	0	0	0	1	0	0	0
.......................................................................................................................................................................................AAATATAAAATCGTGCAAGTTCCT..............................................................................................	24	1	1.00	1	0	0	0	0	0	0	0	0	0	1
.........................................................................................................................................................................................................................TACGACGGACCGCAAATACGCAAGA...........................................................	25	1	1.00	1	0	0	0	0	0	0	0	0	1	0
...TGTGTGTGTGTACAGTCTCCAAGATGG...............................................................................................................................................................................................................................................................................	27	1	1.00	1	0	0	0	0	0	0	0	0	1	0
.........TGTGTACAGTCTCCAAGATGGATAC...........................................................................................................................................................................................................................................................................	25	1	1.00	1	0	0	0	0	0	0	0	0	1	0
...........................................................................................................................................................................................................................CGACGGACCGCAAATACGCAAGATC.........................................................	25	1	1.00	1	0	0	0	0	0	0	0	0	1	0
.................................................AGTCAACCCCGGATAGGCAC........................................................................................................................................................................................................................................	20	1	1.00	1	0	0	1	0	0	0	0	0	0	0
................................................................................TTGCAAATCGCTCCTGTTCAGTCA.....................................................................................................................................................................................................	24	1	1.00	1	0	0	0	0	0	0	0	0	1	0
..................................................................................................................................................................................................................................................TCTACCAGCAGGCGCACTCATGC....................................	23	1	1.00	1	0	0	0	0	0	0	0	0	1	0
...........................................................................................................................................................................................................TCCTCACAAAGCGCTACGACGGAC..........................................................................	24	1	1.00	1	0	0	0	0	0	0	0	0	1	0
.................................................................................................TCAGGACCACCAACAATTTA------------------------------ATAGTG....................................................................................................................................................	26	1	1.00	1	0	0	0	0	0	0	0	0	1	0
................................................................................................................................................................TATAGTATAGTATGTTAAAATATAAA...................................................................................................................	26	1	1.00	1	0	0	0	0	0	0	0	0	1	0
..........................................................................................................................................................................TATGTTAAAATATAAATATAAAATCG.........................................................................................................	26	1	1.00	1	0	0	0	0	0	0	0	0	1	0
...........................................................................TATCCTTGCAAATCGCTCCTGTTCA.........................................................................................................................................................................................................	25	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.............................................................................................................................................................................................AAAATCGTGCAAGTTCCTCACAAAGCGC....................................................................................	28	1	1.00	1	0	0	0	0	0	0	0	0	1	0
.................................................................................TGCAAATCGCTCCTGTTCAGGACCACC.................................................................................................................................................................................................	27	1	1.00	1	0	0	0	0	0	0	0	0	1	0
.................................................................................TGCAAATCGCTCCTGTTCAGGACCACCA................................................................................................................................................................................................	28	1	1.00	1	0	0	0	0	0	0	0	0	1	0
.....TGTGTGTGTACAGTCTCCAAGATGG...............................................................................................................................................................................................................................................................................	25	1	1.00	1	0	0	0	0	0	0	0	0	1	0
.........................................................................................................................................................................................................................................TACGCAAGATCTACCAGCAGGCGC............................................	24	1	1.00	1	0	0	0	0	0	0	0	0	1	0
..........................................................................................................................................................................................................TTCCTCACAAAGCGCTACGACGGAC..........................................................................	25	1	1.00	1	0	0	0	0	0	0	0	0	1	0
.........................GATGGATACTCCCAATCCGGATGGAG..........................................................................................................................................................................................................................................................	26	1	1.00	1	0	0	0	0	0	0	0	0	1	0
....................................................CAACCCCGGATAGGCACTT......................................................................................................................................................................................................................................	19	1	1.00	1	0	1	0	0	0	0	0	0	0	0
..................................................................................................................TTA------------------------------ATAGTGTGTTAAATATAG........................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	1	0
................................................................................TTGCAAATCGCTCCTGTTCAGGACCAC..................................................................................................................................................................................................	27	1	1.00	1	0	0	0	0	0	0	0	0	1	0
...TGTGTGTGTGTACAGTCTCCAAGA..................................................................................................................................................................................................................................................................................	24	1	1.00	1	0	0	0	0	0	0	0	0	1	0
................................................................................................TTCAGGACCACCAACAATTTA------------------------------ATAGA.....................................................................................................................................................	26	1	1.00	1	0	0	0	0	0	0	0	0	1	0
..................................................................................................................................................................................................CGTGCAAGTTCCTCACAAAGCG.....................................................................................	22	1	1.00	1	0	0	0	0	0	0	1	0	0	0
...........................................................................................................................................................................ATGTTAAAATATAAATATAAAATCGT........................................................................................................	26	1	1.00	1	0	0	0	0	0	0	0	0	1	0
...........................................................................TATCCTTGCAAATCGCTCCT..............................................................................................................................................................................................................	20	1	1.00	1	0	0	0	0	0	0	0	0	1	0
..................................TCCCAATCCGGATGGAGTCAACCCCG.................................................................................................................................................................................................................................................	26	1	1.00	1	0	0	0	0	0	0	1	0	0	0
.....TGTGTGTGTACAGTCTCCAAGATGGA..............................................................................................................................................................................................................................................................................	26	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.....................................................................................................................................................................TATAGTATGTTAAAATATAAATA.................................................................................................................	23	1	1.00	1	0	0	0	0	0	0	0	0	1	0
..................................TCCCAATCCGGATGGAGTCAACCCC..................................................................................................................................................................................................................................................	25	1	1.00	1	0	0	0	0	0	0	1	0	0	0
..............................................................................................TGTTCAGGACCACCAACAATTTA------------------------------ATAG......................................................................................................................................................	27	1	1.00	1	0	0	0	0	0	0	0	0	1	0
...........................................................................................................................................................................................................TCCTCACAAAGCGCTACGACGG............................................................................	22	1	1.00	1	0	0	0	0	0	0	0	0	1	0

dpe_1569

scaffold_4:1867914-1868064 -

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-22.8	-22.7	-22.7