dpe_1509

##################################################--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- TCGAGATTTTGGTGCCGAACGACAGTCTGGATCCCCACGATCCTGGGAAAGTGGGTCCTGCTCCGATCTGGCGCCATATATCTGAGATATAGCCTTCCGCCGAATGGTTGGATGTGCAAATCATTCGAATATTTGTTTTTTCTGCACGCTATATCTCAGCCATACGGCAGCCGATCGGAGCGTGGCATGGCTCTGCGTGATCGGGAGAGTCCTGCCGACCGATTGGCACCAGAAAAAAGGACATCCTTTTC ********************************************************************************((.((((((((.......((((((.(((((((.......))))))).))))))............)))))))).))....***************************************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M042 female body	M021 embryo	V042 embryo
........................................................................................................................................TTTTTCTGCACGCTATATCTCAGCCA.........................................................................................	26	0	20	0.35	7	7	0	0
.......................................................................................................................................TTTTTTCTGCACGCTATATCTCAGCCA.........................................................................................	27	0	20	0.20	4	4	0	0
................................................................................................................................................................................................CTGCGTGATCGGGAGAGTC........................................	19	0	20	0.10	2	0	2	0
................................................................................................................................................................................................CTGCGTGATCGGGAGAGTCCTGCC...................................	24	0	20	0.10	2	2	0	0
..................................................................................TGAGATATAGCCTTCCGCCGAATGG................................................................................................................................................	25	0	20	0.10	2	2	0	0
............................................................................................................................................TCTGCACGCTATATCTCAGCCATACG.....................................................................................	26	0	20	0.05	1	1	0	0
..................................................................................TGAGATATAGCCTTCCGCCGAATGGT...............................................................................................................................................	26	0	20	0.05	1	1	0	0
......TTTTGGTGCCGAACGACAGTCTGG.............................................................................................................................................................................................................................	24	0	20	0.05	1	1	0	0
....................................................................................................CGTACGGTTGGATGTGCGA....................................................................................................................................	19	3	20	0.05	1	0	0	1
......................CAGTCTGGTTCCCCACGATCCTGG.............................................................................................................................................................................................................	24	1	20	0.05	1	1	0	0
................................................................................................................................................................................................CTGCGTGATCGGGAGAGTCCTG.....................................	22	0	20	0.05	1	1	0	0

AGCTCTAAAACCACGGCTTGCTGTCAGACCTAGGGGTGCTAGGACCCTTTCACCCAGGACGAGGCTAGACCGCGGTATATAGACTCTATATCGGAAGGCGGCTTACCAACCTACACGTTTAGTAAGCTTATAAACAAAAAAGACGTGCGATATAGAGTCGGTATGCCGTCGGCTAGCCTCGCACCGTACCGAGACGCACTAGCCCTCTCAGGACGGCTGGCTAACCGTGGTCTTTTTTCCTGTAGGAAAAG ***************************************************************************************((.((((((((.......((((((.(((((((.......))))))).))))))............)))))))).))....********************************************************************************	Read size	Hit Count	Total Norm	Total	M042 female body	M021 embryo
........AACCACGGCTTGCTGTCAGACCTA...........................................................................................................................................................................................................................	24	20	0.15	3	3	0
..........CCACGGCTTGCTGTCAGACC.............................................................................................................................................................................................................................	20	20	0.10	2	0	2
.........ACCACGGCTTGCTGTCAGACCTA...........................................................................................................................................................................................................................	23	20	0.10	2	2	0
.......AAACCACGGCTTGCTGTCAGACCTA...........................................................................................................................................................................................................................	25	20	0.10	2	0	2
.....................................................................................................................................................................................CACCGTACCGAGACGCACTAGCCCTCT...........................................	27	17	0.06	1	1	0
..........................................................................................................................................................................................................................GGCTAACCGTGGTCTTTTTTCCTGT........	25	20	0.05	1	1	0
...TCTAAAACCACGGCTTGCTGTCAGA...............................................................................................................................................................................................................................	25	20	0.05	1	1	0
....................................................................................................................................................................................................................ACGGCTGGCTAACCGTGGTCTTT................	23	20	0.05	1	1	0
..................................................................................................................................................................................................................GGACGGCTGGCTAACCGTGGTCTT.................	24	20	0.05	1	1	0
......AAAACCACGGCTTGCTGTCAGACCTA...........................................................................................................................................................................................................................	26	20	0.05	1	1	0

Species	Coordinate	ID	Alignment
droPer2	scaffold_35:172504-172754 -	dpe_1509	TCGAGATTTTGGTGCCGAACGACAGTCTGGATCCCCACGATCCTGGGAAAGTGGGTCCTGCTCCGATCTGGCGCCATATATCTGAGATATAGCCTTCCGCCGAATGGTTGGATGTGCAAATCATTCGAATATTTGTTTTTTCTGCACGCTATATCTCAGCCATACGGCAGCCGATCGGAGCGTGGCATGGCTCTGCGTGATCGGGAGAGTCCTGCCGACCGATTGGCACC-----------AGAAAAAAGGACATCCTTTTC
dp5	XR_group6:9727489-9727739 +		TCGAGATTTTGGTGCCGAACGACAGTCTGGATCCCCACGATCCTGGGAAAGTGGGTCCTGCTCCGATCTGGCGCCATATATCTGAGATATAGCCTTCCGCCGAATGGTTGGATGTGCAAATCATTCGAATATTTGTTTGTTCTGCACGCTATATCTCAGCTATACGGCAGCCGATCGGAGCGTGGCATGGCTCTGCGTGATCGGGAGAGTCCTGCCGACCGATTGGCATC-----------AGAAAAAAGGACATCCTTTTC
droAna3	scaffold_13047:463699-463933 +		ACGATCTTTGGATGCATTTCGACTGATTTTGGGGCGCTGATTCCGAAAATGTAGGGCATTGCCAGATCGGATGAGAAATGGCTGAGATATGGGCATTTGAAAAATGT---------------------------GTACTTTCTTTAGATCATATCTCGGCCATTTCTCAACCGATCTGGCAACGCTGTGCATTTTTGGAATCAGGACTCGGATGTCCTTCGAACTGCATCTTTACTTTTTCGAAATCGAACAACTCATTTTC

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droPer2

TCGAGATTTTGGTGCCGAACGACAGTCTGGATCCCCACGATCCTGGGAAAGTGGGTCCTGCTCCGATCTGGCGCCATATATCTGAGATATAGCCTTCCGCCGAATGGTTGGATGTGCAAATCATTCGAATATTTGTTTTTTCTGCACGCTATATCTCAGCCATACGGCAGCCGATCGGAGCGTGGCATGGCTCTGCGTGATCGGGAGAGTCCTGCCGACCGATTGGCACC-----------AGAAAAAAGGACATCCTTTTC	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
........................................................................................................................................TTTTTCTGCACGCTATATCTCAGCCA....................................................................................................	26	20	0.35	7	0	7	0	0	0	0
.......................................................................................................................................TTTTTTCTGCACGCTATATCTCAGCCA....................................................................................................	27	20	0.20	4	0	4	0	0	0	0
................................................................................................................................................................................................CTGCGTGATCGGGAGAGTC...................................................	19	20	0.10	2	2	0	0	0	0	0
................................................................................................................................................................................................CTGCGTGATCGGGAGAGTCCTGCC..............................................	24	20	0.10	2	0	2	0	0	0	0
..................................................................................TGAGATATAGCCTTCCGCCGAATGG...........................................................................................................................................................	25	20	0.10	2	0	2	0	0	0	0
............................................................................................................................................TCTGCACGCTATATCTCAGCCATACG................................................................................................	26	20	0.05	1	0	1	0	0	0	0
..................................................................................TGAGATATAGCCTTCCGCCGAATGGT..........................................................................................................................................................	26	20	0.05	1	0	1	0	0	0	0
......TTTTGGTGCCGAACGACAGTCTGG........................................................................................................................................................................................................................................	24	20	0.05	1	0	1	0	0	0	0
................................................................................................................................................................................................CTGCGTGATCGGGAGAGTCCTG................................................	22	20	0.05	1	0	1	0	0	0	0

dp5

TCGAGATTTTGGTGCCGAACGACAGTCTGGATCCCCACGATCCTGGGAAAGTGGGTCCTGCTCCGATCTGGCGCCATATATCTGAGATATAGCCTTCCGCCGAATGGTTGGATGTGCAAATCATTCGAATATTTGTTTGTTCTGCACGCTATATCTCAGCTATACGGCAGCCGATCGGAGCGTGGCATGGCTCTGCGTGATCGGGAGAGTCCTGCCGACCGATTGGCATC-----------AGAAAAAAGGACATCCTTTTC	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
.......................................................................................................................................................TATCTCAGCTATACGGCAGCCGA........................................................................................	23	20	0.55	11	0	11	0	0	0	0	0	0	0	0
...........................................TGGGAAAGTGGGTCCTGCTCCGATC..................................................................................................................................................................................................	25	20	0.30	6	0	6	0	0	0	0	0	0	0	0
...........................TGGATCCCCACGATCCTGGGAAAG...................................................................................................................................................................................................................	24	20	0.10	2	0	2	0	0	0	0	0	0	0	0
..................................................................................TGAGATATAGCCTTCCGCCGAATGG...........................................................................................................................................................	25	20	0.10	2	0	2	0	0	0	0	0	0	0	0
................................................................................................................................................................................................................GTCCTGCCGACCGATTGGCATC-----------AG...................	24	15	0.07	1	0	1	0	0	0	0	0	0	0	0
....................................................................................................................................................CTATATCTCAGCTATACGGCAGCC..........................................................................................	24	20	0.05	1	0	1	0	0	0	0	0	0	0	0
.........................................CCTGGGAAAGTGGGTCCTGCTCCGA....................................................................................................................................................................................................	25	20	0.05	1	0	1	0	0	0	0	0	0	0	0
..............................................................................TATCTGAGATATAGCCTTCCG...................................................................................................................................................................	21	20	0.05	1	0	1	0	0	0	0	0	0	0	0
......................CAGTCTGGATCCCCACGATCCT..........................................................................................................................................................................................................................	22	20	0.05	1	0	1	0	0	0	0	0	0	0	0
..................................................................................TGAGATATAGCCTTCCGCCGAATGGT..........................................................................................................................................................	26	20	0.05	1	0	1	0	0	0	0	0	0	0	0
.............................................................................ATATCTGAGATATAGCCTTCCGC..................................................................................................................................................................	23	20	0.05	1	0	1	0	0	0	0	0	0	0	0
...........................................................................................................................................................................................TGGCTCTGCGTGATCGGGAGAGTC...................................................	24	20	0.05	1	0	1	0	0	0	0	0	0	0	0
.......................AGTCTGGATCCCCACGATCCTG.........................................................................................................................................................................................................................	22	20	0.05	1	0	1	0	0	0	0	0	0	0	0
.......................................................................................................................................................................................GGCATGGCTCTGCGTGATCGGGA........................................................	23	20	0.05	1	0	1	0	0	0	0	0	0	0	0
...................................................TGGGTCCTGCTCCGATCTGGCG.............................................................................................................................................................................................	22	20	0.05	1	0	1	0	0	0	0	0	0	0	0
.......................................................................................................................................................TATCTCAGCTATACGGCAGCCGATCGGA...................................................................................	28	20	0.05	1	0	1	0	0	0	0	0	0	0	0
...................................................TGGGTCCTGCTCCGATCTGGCGCCATAA.......................................................................................................................................................................................	28	20	0.05	1	0	1	0	0	0	0	0	0	0	0
....................................................................TGGCGCCATATATCTGAGATATAGCCT.......................................................................................................................................................................	27	20	0.05	1	0	1	0	0	0	0	0	0	0	0
............TGCCGAACGACAGTCTGGATC.....................................................................................................................................................................................................................................	21	20	0.05	1	0	1	0	0	0	0	0	0	0	0
.......................................................................................................................................TTTGTTCTGCACGCTATATCTCAGC......................................................................................................	25	20	0.05	1	0	1	0	0	0	0	0	0	0	0
...............................................................................................................................................................................................TCTGCGTGATCGGGAGAGTCCTGC...............................................	24	20	0.05	1	0	1	0	0	0	0	0	0	0	0
...................................................................................GAGATATAGCCTTCCGCCGAATGG...........................................................................................................................................................	24	20	0.05	1	0	1	0	0	0	0	0	0	0	0
............................................GGGAAAGTGGGTCCTGCTCCGATC..................................................................................................................................................................................................	24	20	0.05	1	0	1	0	0	0	0	0	0	0	0
........................................TCCTGGGAAAGTGGGTCCTGCTCT......................................................................................................................................................................................................	24	20	0.05	1	0	1	0	0	0	0	0	0	0	0
..................................................................................................................................................................TACGGCAGCCGATCGGAGCGTGGC............................................................................	24	20	0.05	1	1	0	0	0	0	0	0	0	0	0
...................................CACGATCCTGGGAAAGTGG................................................................................................................................................................................................................	19	20	0.05	1	0	1	0	0	0	0	0	0	0	0

droAna3

ACGATCTTTGGATGCATTTCGACTGATTTTGGGGCGCTGATTCCGAAAATGTAGGGCATTGCCAGATCGGATGAGAAATGGCTGAGATATGGGCATTTGAAAAATGT---------------------------GTACTTTCTTTAGATCATATCTCGGCCATTTCTCAACCGATCTGGCAACGCTGTGCATTTTTGGAATCAGGACTCGGATGTCCTTCGAACTGCATCTTTACTTTTTCGAAATCGAACAACTCATTTTC	Size	Hit Count	Total Norm	Total	M044 Female-body	M058 Embryo	V039 Embryo	V055 Head	V105 Male-body	V106 Head
................................GGCGCTGATTCCGAAAATGT..................................................................................................................................................................................................................	20	20	0.35	7	0	0	0	0	2	5
ACGATCTTTGGATGCATTTCGACT..............................................................................................................................................................................................................................................	24	20	0.30	6	2	4	0	0	0	0
ACGATCTTTGGATGCATTTCGAC...............................................................................................................................................................................................................................................	23	20	0.30	6	5	1	0	0	0	0
....................................................................................................AAAATGT---------------------------GTACTTTCTTTG....................................................................................................................	19	4	0.25	1	0	0	1	0	0	0
................................................................................................................................................................CATTTCTCAACCGATCTGGCA.................................................................................	21	7	0.14	1	0	0	0	0	1	0
....................................................................................................................................................TCATATCTCGGCCATTTCTCAACCGATCT.....................................................................................	29	7	0.14	1	1	0	0	0	0	0
....................................................................................................................................................TCATATCTCGGCCATTTCTCAAC...........................................................................................	23	8	0.13	1	1	0	0	0	0	0
................................................ATGTAGGGCATTGCCAGATCGG................................................................................................................................................................................................	22	20	0.10	2	0	0	0	0	1	1
....................................................AGGGCATTGCCAGATCGGATG.............................................................................................................................................................................................	21	20	0.10	2	1	1	0	0	0	0
..............................................................................................................................................................................................ATTTTTGGAATCAGGACTCGG...................................................	21	10	0.10	1	0	0	0	1	0	0
....................................................................................................................................................................................AACGCTGTGCATTTTTGGAAT.............................................................	21	20	0.10	2	0	0	0	0	0	2
.........................................................ATTGCCAGATCGGATGAGAAAT.......................................................................................................................................................................................	22	20	0.10	2	0	0	1	0	1	0
ACGATCTTTGGATGCATTTCGA................................................................................................................................................................................................................................................	22	20	0.10	2	2	0	0	0	0	0
................................................................................................................................................................................................................CGGATGTCCTTCGAACTGCAT.................................	21	14	0.07	1	0	0	0	0	0	1
....................................................................................................................................................................................................GGAATCAGGACTCGGATGTCC.............................................	21	15	0.07	1	0	0	0	0	0	1
..................................................................................................................................................................................................TTGGAATCAGGACTCGGATGTC..............................................	22	15	0.07	1	0	0	0	0	0	1
...................................................................................................................................................................................................TGGAATCAGGACTCGGATGTC..............................................	21	15	0.07	1	0	0	0	0	0	1
..................................................................................................................................................................................................TTGGAATCAGGACTCGGATGT...............................................	21	15	0.07	1	0	0	0	0	0	1
.............................TGGGGCGCTGATTCCGAAAATGTAGG...............................................................................................................................................................................................................	26	20	0.05	1	1	0	0	0	0	0
............................................................GCCAGATCGGATGAGAAATGG.....................................................................................................................................................................................	21	20	0.05	1	0	0	0	0	1	0
...................................................................................................................................................................................CAACGCTGTGCATTTTTGGAAT.............................................................	22	20	0.05	1	0	0	0	1	0	0
......................................GATTCCGAAAATGTAGGGCATTGCCAGA....................................................................................................................................................................................................	28	20	0.05	1	1	0	0	0	0	0
.......................................ATTCCGAAAATGTAGGGCATTG.........................................................................................................................................................................................................	22	20	0.05	1	1	0	0	0	0	0
ACGATCTTTGGATGCATTTCGACA..............................................................................................................................................................................................................................................	24	20	0.05	1	1	0	0	0	0	0
...................................................................................................................................................................................CAACGCTGTGCATTTTTGGA...............................................................	20	20	0.05	1	0	0	1	0	0	0
.....................................................................................................................................................................................ACGCTGTGCATTTTTGGAATC............................................................	21	20	0.05	1	0	0	0	1	0	0
...................................................TAGGGCATTGCCAGATCGGAT..............................................................................................................................................................................................	21	20	0.05	1	0	1	0	0	0	0
..........................................................TTGCCAGATCGGATGAGAAAT.......................................................................................................................................................................................	21	20	0.05	1	0	1	0	0	0	0
...........................................................................................................................................................................................................................................TTTTTCGAAATCGAACAACTCATTTTC	27	20	0.05	1	1	0	0	0	0	0
........................................................CATTGCCAGATCGGATGAGAAA........................................................................................................................................................................................	22	20	0.05	1	0	1	0	0	0	0
...................................................TAGGGCATTGCCAGATCGGA...............................................................................................................................................................................................	20	20	0.05	1	0	0	0	0	1	0
...............................................AATGTAGGGCATTGCCAGATCG.................................................................................................................................................................................................	22	20	0.05	1	1	0	0	0	0	0
..........................................................TTGCCAGATCGGATGAGAA.........................................................................................................................................................................................	19	20	0.05	1	0	0	1	0	0	0
............................................GAAAATGTAGGGCATTGCCAGATCG.................................................................................................................................................................................................	25	20	0.05	1	1	0	0	0	0	0
.................................GCGCTGATTCCGAAAATGTA.................................................................................................................................................................................................................	20	20	0.05	1	1	0	0	0	0	0
..........................................................................................................................................................................................GTGCATTTTTGGAATCAGGAC.......................................................	21	20	0.05	1	0	0	0	0	0	1
ACGATCTTTGGATGCATTTCG.................................................................................................................................................................................................................................................	21	20	0.05	1	1	0	0	0	0	0
..................................................................................................................................................................................GCAACGCTGTGCATTTTTGG................................................................	20	20	0.05	1	1	0	0	0	0	0
.............................................................CCAGATCGGATGAGAAATGGC....................................................................................................................................................................................	21	20	0.05	1	0	1	0	0	0	0
...................................................................................................................................................................................CAACGCTGTGCATTTTTGG................................................................	19	20	0.05	1	1	0	0	0	0	0

dpe_1509

scaffold_35:172554-172704 -

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-24.1	-24.1