dpe_144

ATCATCATATTGTACTGTGAAGAGAACACTGTTTTATGCGGTTGAAGTAGTGGGTGGAAAGGCTAAAATATTTTTATATTTTCAATTGAATAAATTAGATTTTCTGCCAACTTCTGCGATTCGCATCGACCCCTGTTTGTGCACTCTCGGGAGCGATCGTTTC *********************************(((((((((.((.(((.((((((((..((((..(((((..............)))))..))))..)))))))).))).)).))).)))))).**********************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M021 embryo	M042 female body	V111 male body	V057 head	V050 head
..........................................................................................TAAATTAGATTTTCTGCCAACT...................................................	22	0	1	27.00	27	14	12	1	0	0
..................................................TGGGTGGAAAGGCTAAAATATT...........................................................................................	22	0	1	3.00	3	0	1	1	0	1
..........................................................................................TAAATTAGATTTTCTGCCAACC...................................................	22	1	1	2.00	2	2	0	0	0	0
..................................................TGGGTGGAAAGGCTAAAATAT............................................................................................	21	0	1	2.00	2	0	0	2	0	0
..........................................................................................TAAATTAGATTTTCTGCCAACG...................................................	22	1	1	2.00	2	2	0	0	0	0
..................................................TGGGTGGAAAGGCTAAAATATTTCC........................................................................................	25	2	1	1.00	1	0	1	0	0	0
..................................................TGGGTGGAAAGGCTAAAATA.............................................................................................	20	0	1	1.00	1	0	0	1	0	0
.............................................................................................ATTAGATTTTCTGCCAACT...................................................	19	0	1	1.00	1	1	0	0	0	0
..........................................................................................TAAATTAGATTTTCTGCCAA.....................................................	20	0	1	1.00	1	0	0	1	0	0
...........................................................................................AAATTAGATTTTCTGCCAACT...................................................	21	0	1	1.00	1	1	0	0	0	0
..........................................................................................TAAATTAGATTTTCTGCCAAC....................................................	21	0	1	1.00	1	0	1	0	0	0
..................................................TGGGTGGAAAGGCTAAAATATTTTTATA.....................................................................................	28	0	1	1.00	1	0	0	0	1	0
..............................GTTTTATGCGGTTGAAGTAG.................................................................................................................	20	0	2	0.50	1	0	0	1	0	0
....TGATATTGTAAGGTGAAGA............................................................................................................................................	19	3	20	0.05	1	0	0	0	1	0

TAGTAGTATAACATGACACTTCTCTTGTGACAAAATACGCCAACTTCATCACCCACCTTTCCGATTTTATAAAAATATAAAAGTTAACTTATTTAATCTAAAAGACGGTTGAAGACGCTAAGCGTAGCTGGGGACAAACACGTGAGAGCCCTCGCTAGCAAAG **********************************(((((((((.((.(((.((((((((..((((..(((((..............)))))..))))..)))))))).))).)).))).)))))).*********************************	Read size	# Mismatch	Hit Count	Total Norm	Total	V057 head
..........................................................................................................................................CACCTGAGAACCCCCGCTAG.....	20	3	6	0.17	1	1
...........................................................................................................................................ACCTGAGAGCCCCCTCTAG.....	19	3	15	0.07	1	1

Species	Coordinate	ID	Type	Alignment
droPer2	scaffold_13:197817-197979 +	dpe_144	confident	ATCATCATATTGTACTGTGAAGAGAACACTGTTTTATGCGGTTGAAGTAGTGGGTGGAAAGGCTAAAATATTTTTATATTTTCAATTGAATAAATTAGATTTTCTGCCAACTTCTGCGATTCGCATCGACCCCTGTTTGTGCACTCTCGGGAGCGATCGTTTC
dp5	XL_group1e:8366435-8366597 -	dps_73	confident	ATCATCATAGTATACTGTGAAGAAAACACTGTTTCATGCGGTTGAAGTAGTGGGTAGAAAGGCTAAAATATTTGTATATTTTCAATTGAATAAATTAGATTTTCTGCCAGCTTCTGCGATTCGCATCGACCCCTGTTTATGCGCTCTCGAGAGCGATCGTTTC

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droPer2

ATCATCATATTGTACTGTGAAGAGAACACTGTTTTATGCGGTTGAAGTAGTGGGTGGAAAGGCTAAAATATTTTTATATTTTCAATTGAATAAATTAGATTTTCTGCCAACTTCTGCGATTCGCATCGACCCCTGTTTGTGCACTCTCGGGAGCGATCGTTTC	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
..........................................................................................TAAATTAGATTTTCTGCCAACT...................................................	22	1	27.00	27	14	12	0	0	0	1
..................................................TGGGTGGAAAGGCTAAAATATT...........................................................................................	22	1	3.00	3	0	1	0	1	0	1
..........................................................................................TAAATTAGATTTTCTGCCAACC...................................................	22	1	2.00	2	2	0	0	0	0	0
..................................................TGGGTGGAAAGGCTAAAATAT............................................................................................	21	1	2.00	2	0	0	0	0	0	2
..........................................................................................TAAATTAGATTTTCTGCCAACG...................................................	22	1	2.00	2	2	0	0	0	0	0
..................................................TGGGTGGAAAGGCTAAAATATTTCC........................................................................................	25	1	1.00	1	0	1	0	0	0	0
..................................................TGGGTGGAAAGGCTAAAATA.............................................................................................	20	1	1.00	1	0	0	0	0	0	1
.............................................................................................ATTAGATTTTCTGCCAACT...................................................	19	1	1.00	1	1	0	0	0	0	0
..........................................................................................TAAATTAGATTTTCTGCCAA.....................................................	20	1	1.00	1	0	0	0	0	0	1
...........................................................................................AAATTAGATTTTCTGCCAACT...................................................	21	1	1.00	1	1	0	0	0	0	0
..........................................................................................TAAATTAGATTTTCTGCCAAC....................................................	21	1	1.00	1	0	1	0	0	0	0
..................................................TGGGTGGAAAGGCTAAAATATTTTTATA.....................................................................................	28	1	1.00	1	0	0	0	0	1	0
..............................GTTTTATGCGGTTGAAGTAG.................................................................................................................	20	2	0.50	1	0	0	0	0	0	1

dp5

ATCATCATAGTATACTGTGAAGAAAACACTGTTTCATGCGGTTGAAGTAGTGGGTAGAAAGGCTAAAATATTTGTATATTTTCAATTGAATAAATTAGATTTTCTGCCAGCTTCTGCGATTCGCATCGACCCCTGTTTATGCGCTCTCGAGAGCGATCGTTTC	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
..........................................................................................TAAATTAGATTTTCTGCCAGCT...................................................	22	1	148.00	148	2	19	18	11	9	8	5	0	74	2
..........................................................................................TAAATTAGATTTTCTGCCAGC....................................................	21	1	57.00	57	0	6	3	2	0	1	1	2	41	1
..........................................................................................TAAATTAGATTTTCTGCCAG.....................................................	20	1	35.00	35	0	2	0	0	0	0	0	0	33	0
...............................TTTCATGCGGTTGAAGTAG.................................................................................................................	19	1	30.00	30	0	0	0	0	0	0	2	0	28	0
..........................................................................................TAAATTAGATTTTCTGCCAGCC...................................................	22	1	16.00	16	0	0	12	1	0	0	0	0	3	0
..........................................................................................TAAATTAGATTTTCTGCCAGT....................................................	21	1	5.00	5	0	0	0	0	0	0	0	0	5	0
..................................................TGGGTAGAAAGGCTAAAATATTTGTA.......................................................................................	26	1	4.00	4	0	0	0	0	0	0	0	0	4	0
...........................................................................................AAATTAGATTTTCTGCCAGCT...................................................	21	1	3.00	3	0	3	0	0	0	0	0	0	0	0
..................................................TGGGTAGAAAGGCTAAAATATT...........................................................................................	22	1	3.00	3	0	0	0	0	0	0	0	0	3	0
..................................................TGGGTAGAAAGGCTAAAATATC...........................................................................................	22	1	2.00	2	0	0	0	0	0	0	0	0	2	0
..........................................................................................TAAATTAGATTTTCTGCCAGCA...................................................	22	2	2.00	4	0	0	0	0	0	1	0	0	3	0
..........................................................................................TAAATTAGATTTTCTGCCAGCTTCC................................................	25	1	2.00	2	0	0	0	0	0	0	0	0	2	0
..........................................................................................TAAATTAGATTTTCTGCCAGCTCT.................................................	24	1	2.00	2	0	0	0	0	0	0	0	0	2	0
..............................GTTTCATGCGGTTGAAGTAG.................................................................................................................	20	1	2.00	2	0	0	0	0	0	0	0	0	2	0
............................................................................................AATTAGATTTTCTGCCAGCT...................................................	20	1	1.00	1	0	0	1	0	0	0	0	0	0	0
..........................................................................................TAAATTAGATTTTCTGCCAGCCC..................................................	23	1	1.00	1	0	0	0	0	0	0	0	0	1	0
..................................................TGGGTAGAAAGGCTAAAATAT............................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	1	0
..........................................................................................TAAATTAGATTTTCTGCCAGCTG..................................................	23	1	1.00	1	0	1	0	0	0	0	0	0	0	0
...............................................................................................TAGATTTTCTGCCAGCTA..................................................	18	1	1.00	1	0	0	0	0	0	0	0	0	1	0
..........................................................................................TAAATTAGATTTTCTGCCAGCTT..................................................	23	1	1.00	1	0	0	0	0	0	0	1	0	0	0
..........................................................................................TAAATTAGATTTTCTGCCAGCG...................................................	22	1	1.00	1	0	0	0	0	0	0	0	0	1	0
..........................................................................................TAAATTAGATTTTCTGCCAGA....................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	1	0
................................TTCATGCGGTTGAAGTAG.................................................................................................................	18	1	1.00	1	0	0	0	0	0	0	0	0	1	0
..........................................................................................TAAATTAGATTTTCTGCCA......................................................	19	1	1.00	1	0	0	0	0	0	0	0	0	1	0
..................................................TGGGTAGAAAGGCTAAAATA.............................................................................................	20	1	1.00	1	0	0	0	0	0	0	0	0	1	0
..........................................................................................TAAATTAGATTTTCTGCCAGCTC..................................................	23	1	1.00	1	0	0	0	0	0	0	0	0	1	0
.......................................................................................GAATAAATTAGATTTTCTGCC.......................................................	21	2	0.50	1	0	0	0	0	0	0	0	0	1	0
..........................................................................................TAAATTAGATTTTCTGA........................................................	17	4	0.25	1	0	0	0	0	0	0	0	0	1	0

dpe_144

scaffold_13:197867-197929 +

confident

Testes-restricted

intergenic

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-24.9	-24.6	-24.6