| GAA-ATAATAAT-----------------------------------------------------------------------G---------------------------------GGTA--TTTAATAGCCGAGTCA-----------C-TCCAT---T-----TT---------AGCTT-TT---TATCTTTGTATACCCGTTACTCG----TA--GAGT-AAAAGGGTA------TACTAGATTCGTTGA-------AAAATGTGTAACAGGCAGAAGGATGCGTTTCCGACCATATAAAGTATGTA-CATATATTCTTGATCAGGATCAATAGCCGAGTCGATCTGGCCATGTCCGTCTGTCCGGA-TG----TCCGTATG---- | Size | Hit Count | Total Norm | Total | M023 Head | M024 Male-body | M025 Embryo | M053 Female-body | O001 Testis | O002 Head | SRR553485 Ovary | SRR553486 Ovary | SRR553487 Ovary | SRR553488 Ovary | SRR618934 Ovary | V044 Embryo | SRR902008 Ovary | SRR902009 Testis | SRR1275487 Male larvae | SRR1275485 Male prepupae | SRR1275483 Male prepupae |
| .............................................................................................................................................................................................................TACTCG----TA--GAGT-AAAAGGGTA------....................................................................................................................................................... | 21 | 20 | 7.35 | 147 | 0 | 0 | 0 | 0 | 147 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..............................................................................................................................................................................................................................T-AAAAGGGTA------TACTAGATTCGTTGA-------AA............................................................................................................................... | 27 | 20 | 3.70 | 74 | 0 | 0 | 0 | 0 | 0 | 0 | 74 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .....................................................................................................................................................................................................................................................................................................................................TCAGGATCAATAGCCGAGTCGATC......................................... | 24 | 20 | 2.20 | 44 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 44 | 0 | 0 | 0 | 0 | 0 | 0 |
| .......................................................................................................................................................................................................................TA--GAGT-AAAAGGGTA------TACTAGATTCGT........................................................................................................................................... | 27 | 20 | 1.45 | 29 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 29 | 0 | 0 | 0 | 0 | 0 | 0 |
| .........................................................................................................................................................................................................................................................................TGTGTAACAGGCAGAAGGATGCGT..................................................................................................... | 24 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...................................................................................................................................................................................................................................................................................................................................................CGAGTCGATCTGGCCATGTCCGTCT.......................... | 25 | 20 | 0.75 | 15 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 15 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...............................................................................................................................................................................................................................................................................................................................TCTTGATCAGGATCAATAGC................................................... | 20 | 20 | 0.55 | 11 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 11 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...............................................................................................................................................................................................................................................................................................................................TCTTGATCAGGATCAATAGCCGAGTC............................................. | 26 | 20 | 0.50 | 10 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 9 | 0 | 0 | 0 | 0 | 0 | 0 |
| ............................................................................................................................................................................................................................AGT-AAAAGGGTA------TACTAGATTCGTTGA-------A................................................................................................................................ | 28 | 20 | 0.45 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .............................................................................................................................................................................................................................................................................TAACAGGCAGAAGGATGCGT..................................................................................................... | 20 | 5 | 0.40 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
| ....................................................................................................................................................................................................................................GGGTA------TACTAGATTCGTTGA-------AAAA............................................................................................................................. | 24 | 5 | 0.40 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .......................................................................................................................................................................................................................TA--GAGT-AAAAGGGTA------....................................................................................................................................................... | 15 | 20 | 0.35 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
| .................................................................................................................................................................................................................................................................................................................................TTGATCAGGATCAATAGCCGAGTCGATC......................................... | 28 | 20 | 0.35 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...........................................................................................................................................................................................................................................................................................................................ATATTCTTGATCAGGATCAATAGCC.................................................. | 25 | 20 | 0.30 | 6 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
| ........................................................................................................................................................................................................................................A------TACTAGATTCGTTGA-------AAAA............................................................................................................................. | 20 | 7 | 0.29 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 |
| .....................................................................................................................................................................................................................................................................................................................................TCAGGATCAATAGCCGAGTCGATCTGGC..................................... | 28 | 20 | 0.25 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
| .................................................................................................................................................................................................................................................................................................................................................GCCGAGTCGATCTGGCCATGTCCGTC........................... | 26 | 20 | 0.20 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
| .................................................................................................................................................................................................................................................................................AGGCAGAAGGATGCGTT.................................................................................................... | 17 | 5 | 0.20 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ................................................................................................................................................................................................................................AAAAGGGTA------TACTAGATTCGTTGA-------AA............................................................................................................................... | 26 | 20 | 0.20 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..............................................................................................................................................................................................................................................................................AACAGGCAGAAGGATGCGT..................................................................................................... | 19 | 5 | 0.20 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..................................................................................................................................................................................................................................................................................GGCAGAAGGATGCGTT.................................................................................................... | 16 | 6 | 0.17 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ................................................................................................................................................................................................................................................................................CAGGCAGAAGGATGCG...................................................................................................... | 16 | 6 | 0.17 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ................................................................................................................................................................................................TTTGTATACCCGTTACTCG----TA--GAGT-A..................................................................................................................................................................... | 26 | 6 | 0.17 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ............................................................................................................................................................................................................................................................................GTAACAGGCAGAAGGATG........................................................................................................ | 18 | 6 | 0.17 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...............................................................................................................................................................................................................................................................................ACAGGCAGAAGGATG........................................................................................................ | 15 | 6 | 0.17 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...............................................................................................................................................................................................................................................................................................................................................TAGCCGAGTCGATCTGGCCATGTC............................... | 24 | 20 | 0.15 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...................................................................................................................................................................................................................................................................................................................................................CGAGTCGATCTGGCCATGTCCGTCTG......................... | 26 | 20 | 0.15 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...................................................................................................................................................................................................................................................................................................................................................CGAGTCGATCTGGCC.................................... | 15 | 20 | 0.15 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..................................................................................................................................................................................................................................................................................GGCAGAAGGATGCGT..................................................................................................... | 15 | 7 | 0.14 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...............................................................................................................................................................................................................................................TACTAGATTCGTTGA-------AAAA............................................................................................................................. | 19 | 7 | 0.14 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ......................................................................................................................................................................................................................................GTA------TACTAGATTCGTTGA-------AAAA............................................................................................................................. | 22 | 7 | 0.14 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ......................................................................................................................................................................................................................................................................................GAAGGATGCGTTTCCGAC.............................................................................................. | 18 | 8 | 0.13 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| .................................................................................................................................................................................................................................................................................................................................TTGATCAGGATCAATAGCCGAGTCGAT.......................................... | 27 | 20 | 0.10 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
| ......................................................................................................................................................................................................................................................................................................................................CAGGATCAATAGCCGAGTCGATC......................................... | 23 | 20 | 0.10 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...................................................................................................................................................................................................GTATACCCGTTACTCG----TA--GAG........................................................................................................................................................................ | 21 | 10 | 0.10 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
| .............................................................................................................................................................................................................TACTCG----TA--GAGT-AAAAGGGTA------TACTAGA................................................................................................................................................ | 28 | 20 | 0.10 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
| ..............................................................................................................................................................................................................................................................................................................................TTCTTGATCAGGATCAA....................................................... | 17 | 20 | 0.10 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...................................................................................................................................................................................................................................................................................................................................................CGAGTCGATCTGGCCATGTC............................... | 20 | 20 | 0.10 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
| ............................................................................................................................................................................................................................................................................................................................................CAATAGCCGAGTCGATCTGGCCATGTC............................... | 27 | 20 | 0.10 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...................................................................................................................................................................................................................................................................................................................................................CGAGTCGATCTGGCCATGTCCGTC........................... | 24 | 20 | 0.10 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 |
| .....................................................................................................................................................................................................................................................................................................................................TCAGGATCAATAGCCGAGTCGAT.......................................... | 23 | 20 | 0.10 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ............................................................................................................................................................................................................................................................................................................................TATTCTTGATCAGGATCAATAGCCGA................................................ | 26 | 20 | 0.10 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .....................................................................................................................................................................................................................................................................................................................TA-CATATATTCTTGATCAGGATCAAT...................................................... | 26 | 14 | 0.07 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..................................................................................................................................................................................................TGTATACCCGTTACT..................................................................................................................................................................................... | 15 | 18 | 0.06 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...................................................................................................................................................................................................................................................................................................................................GATCAGGATCAATAGCCGAGTCGAT.......................................... | 25 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ......................................................................................................................................................................................................................................................................................................................................................GTCGATCTGGCCATGTCCGT............................ | 20 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...............................................................................................................................................................................................................................................................................................................................................TAGCCGAGTCGATCTGGCCATGTCCGT............................ | 27 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ......................................................................................................................................................................................................................................................................................................................................CAGGATCAATAGCCGAGTCGATCTGGC..................................... | 27 | 20 | 0.05 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..................................................................................................................................................................................................................................................................................................................................................CCGAGTCGATCTGGCCATGTCCGTC........................... | 25 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...............................................................................................................................................................................................................................................................................................................................................TAGCCGAGTCGATCTGGCCATGTCCGTCTG......................... | 30 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .....................................................................................................................................................................................................................................................................................................................................................AGTCGATCTGGCCATGTCCGT............................ | 21 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ......................................................................................................................................................................................................................................................................................................................................CAGGATCAATAGCCGAGTCGATCTC....................................... | 25 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ................................................................................................................................................................................................................TCG----TA--GAGT-AAAAGGGTA------TACTA.................................................................................................................................................. | 23 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .......................................................................................................................................................................................................................................................................................................................................AGGATCAATAGCCGAGTCGATCTGG...................................... | 25 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..............................................................................................................................................................................................................................................................................................................................TTCTTGATCAGGATC......................................................... | 15 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ......................................................................................................................................................................................................................................................................................................................................CAGGATCAATAGCCGAGTCGATCTG....................................... | 25 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..................................................................................................................................................................................................................................................................................................................................................CCGAGTCGATCTGGCCATGTC............................... | 21 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...................................................................................................................................................................................................................................................................................................................................................CGAGTCGATCTGGCCATGTCCGTCTGT........................ | 27 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ................................................................................................................................................................................................................................................................................................................................................AGCCGAGTCGATCTGGCCAT.................................. | 20 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...............................................................................................................................................................................................................................................................................................................................TCTTGATCAGGATCAATAGCCGAGTCGAT.......................................... | 29 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...........................................................................................................................................................................................................................................................................................................................................TCAATAGCCGAGTCGATCTG....................................... | 20 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| .............................................................................................................................................................................................................................GT-AAAAGGGTA------TACTAGATTCGTTGA-------A................................................................................................................................ | 27 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
| ................................................................................................................................................................................................................................................................................................................................................AGCCGAGTCGATCTGG...................................... | 16 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ......................................................................................................................................................................................................................................................................................................................................................GTCGATCTGGCCATGTCCGTC........................... | 21 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ............................................................................................................................................................................................................................................................................................................................................CAATAGCCGAGTCGATCTGG...................................... | 20 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
| ................................................................................................................................................................................................................................................................................................................................CTTGATCAGGATCAATAGCCGAGTCGA........................................... | 27 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .............................................................................................................................................................................................................................................................................................................................................AATAGCCGAGTCGATCTGGCCATGTCC.............................. | 27 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |