dpe_1378

CDS [Dper\GL21640-cds]; exon [dper_GLEANR_3733:4]; exon [dper_GLEANR_3733:3]; CDS [Dper\GL21640-cds]; intron [Dper\GL21640-in]

##################################################-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------#############------------------------------------- CCACCATCGCCGCAATCTCCAGGACAAAATATACCTTCCTTCCCTCACGGGTAAGCTGAAACGGGCCAACCAAACGGCTTCTACTTTCGCAATCAGAAATCTCCAAAACCAGGTGTTAACGTTGAACGTCGGCGAAATGTCGAATAATTTAATAATCTAATAAATCACTATCCATTAATACCTCAAATAATGGCAAACAACTTTTTTGATTTTCATTGCAAAACTCCGAAATATCTCAAAATTTGCGATGTATGTTGACTTTTTTCCTTGACTGTATATTTTCTTGAATTTCCACAATATTTTGCAACTTATTTTCTTCTTACAGAGACGAAAAATAAGTATCGGGATCAGGATATACAGGAAATCAATTATATG ************************************************(((((..((((.(((((........)))))..........(((((((((.........((((((((((((((...)))(((((.....)))))...............................))))))))........))).........)))))))))..(((((((((.....((......))....))))))))).((((((......(((...((..........))..)))......))))))............))))..)))))..************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M042 female body	M021 embryo
...CCATCGGCGCAATCTTCAGGAAA.............................................................................................................................................................................................................................................................................................................................................................	23	3	1	1.00	1	1	0
............................................................................................................................................................................................................................................................TGTTGACTTTTTTCCTTGACTGTATA.................................................................................................	26	0	6	0.50	3	3	0
............................................................................................................................................................................................................................................................TGTTGACTTTTTTCCTTGACTGT....................................................................................................	23	0	15	0.27	4	4	0
............................................................................................................................................................................................................................................................TGTTGACTTTTTTCCTTGACTGTAT..................................................................................................	25	0	7	0.14	1	1	0
............................................................................................................................................................................................................................................................TGTTGACTTTTTTCCTTGACTGTA...................................................................................................	24	0	15	0.13	2	2	0
.............................................................................................................................................................................................................................................................GTTGACTTTTTTCCTTGACTGT....................................................................................................	22	0	15	0.13	2	2	0
............................................................................................................................................................................................................................................................TGTTGACTTTTTTCCTTGACTGTAAA.................................................................................................	26	1	12	0.08	1	1	0
........................................................................................................................................................................................................................................................TGTATGTTGACTTTTTTCCTTGACTG.....................................................................................................	26	0	13	0.08	1	1	0
............................................................................................................................................................................................................................................................TGTTGACTTTTTTCCTTGACTG.....................................................................................................	22	0	15	0.07	1	1	0
...........................................................................................................................................................................................................TTTTGATCTTCATTGAAAAGC.......................................................................................................................................................	21	3	15	0.07	1	0	1
........................................................................................................................................................................................AAACAATGGCAAGCACCTTT...........................................................................................................................................................................	20	3	20	0.05	1	1	0

GGTGGTAGCGGCGTTAGAGGTCCTGTTTTATATGGAAGGAAGGGAGTGCCCATTCGACTTTGCCCGGTTGGTTTGCCGAAGATGAAAGCGTTAGTCTTTAGAGGTTTTGGTCCACAATTGCAACTTGCAGCCGCTTTACAGCTTATTAAATTATTAGATTATTTAGTGATAGGTAATTATGGAGTTTATTACCGTTTGTTGAAAAAACTAAAAGTAACGTTTTGAGGCTTTATAGAGTTTTAAACGCTACATACAACTGAAAAAAGGAACTGACATATAAAAGAACTTAAAGGTGTTATAAAACGTTGAATAAAAGAAGAATGTCTCTGCTTTTTATTCATAGCCCTAGTCCTATATGTCCTTTAGTTAATATAC ************************************************(((((..((((.(((((........)))))..........(((((((((.........((((((((((((((...)))(((((.....)))))...............................))))))))........))).........)))))))))..(((((((((.....((......))....))))))))).((((((......(((...((..........))..)))......))))))............))))..)))))..************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M042 female body	V111 male body	V050 head	V042 embryo
............................................................................................................................................................................................................................................................ACAACTGAAAAAAGGAACTGACATAT.................................................................................................	26	0	6	1.67	10	6	4	0	0
...........................................................TTGCCCGATTGGTGTGCCGT........................................................................................................................................................................................................................................................................................................	20	3	4	0.25	1	0	0	1	0
...........................................................................................................................................................................................................................................................TACAACTGAAAAAAGGAACTGACATAT.................................................................................................	27	0	6	0.17	1	1	0	0	0
..........................................................................................................................................................................................................................................................ATACAACTGAAAAAAGGAACTGACATAT.................................................................................................	28	0	6	0.17	1	1	0	0	0
.............................................................................................................................................................................................................................................................CAACTGAAAAAAGGAACTGACATAT.................................................................................................	25	0	6	0.17	1	0	0	0	1
................................................................................................................................................................................................................................................................CTGAAAAAATGAACTGTCATAA.................................................................................................	22	3	12	0.08	1	0	0	1	0
........................................................................................................................................................................................................................................................ACATACAACTGAAAAAAGGAACTGA......................................................................................................	25	0	13	0.08	1	1	0	0	0

Species	Coordinate	ID	Alignment
droPer2	scaffold_3:4364621-4364995 -	dpe_1378	CCACCATCGCCGCAATCTCCAGGACAAAATATACCTTCCTTCCCTCACGGGTAAGCTGAAACGGGCCAACCAAACGGCTTCTACTTTCGCAATCAGAAATCTCCAAAACCAGGTGTTAACGTTGAACGTCGGCGAAATGTCGAATAA-------TTTAATAATCTAATAAATCACTATCCATTAATACCTCAAATAATGGCAAAC-AACTTTTTTGATTTTCATTGCAAAACTCCGAAATATCTCAAAATTTGCGATGTATGTTGACTTTTTTCCTTGACTGTATAT----TTTCTTGAATT----------------------TCCACAATATTTTGCAACTTATTTTCTTCTTACAGAGACGAAAAATAAGTATCGGGATCAGGATATACAGGAAATCAATTATATG
dp5	2:21580697-21581079 -		CCACCATCGCCGCAATCTCCAGGACAAAATATACCTTCCTTCCCTCGCGGGTAAGGTGAAACGGCCCAACCAAACGGCTTCTACTTTCGCAATCAGAAATCTCCAAAACCAGGTGTTAACGTTGAACGTCGGCGAAATGTCGAATAATTTATAATTTAATAATCTAATAAATCACTATCCATTAATATCTCAAATAATGGCAAACCAACTTGTTTGATTTTGATTGCAAAACTCCGAAATATCTCAAAATTGGTGCTGTATGTT-ACTCTTTTCCTTGACTGTATAT----TTTCTTGAATTTTCGCTCTGTTTTAAAGCTATTTCAACAATATTATGAAACTTATTTTCTTCTTACAGAGACGAAA--------------TCAG-------AGGAAATCAATTATATG
droWil2	scf2_1100000004961:351341-351478 +		TAACTTTTG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GTCCTTTAACTTATGACATACTTACATTTTTAATTTGTATTGCAAAAAGCCGAATTATAGCAAAATTTGAGATGTATGTTGACTTTATTCCTTGAGTGTATACTCATTTTTAAAAATTGTTTTTCAATT------------------------------------------------------------------------------------------------
droMoj3	scaffold_6482:100252-100368 -		TAATGATTC---------------------------------------------------------------------------------------------------------------------------------------------------------------------CTATGTATTAATGTTCTAACTATTTGCC-----TTATTTTTTATTTTCGTTTCGAAACCCCGAAATATCGAAAAATTGGTGGTATATGTTGACTATTTTCCTTCACTATAAAT--------------------------------------------------------------------------------------------------------------------------
droRho1	scf7180000779349:15298-15333 -		CCACCTCCGCAGAGTCCTCCCGGAT-------ATCCACCTCCC------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droEug1	scf7180000409766:136236-136271 +		CCACCTACTCAAAGTTCTCCCGGAT-------ATCTTCCCCCC------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droPer2

CCACCATCGCCGCAATCTCCAGGACAAAATATACCTTCCTTCCCTCACGGGTAAGCTGAAACGGGCCAACCAAACGGCTTCTACTTTCGCAATCAGAAATCTCCAAAACCAGGTGTTAACGTTGAACGTCGGCGAAATGTCGAATAA-------TTTAATAATCTAATAAATCACTATCCATTAATACCTCAAATAATGGCAAAC-AACTTTTTTGATTTTCATTGCAAAACTCCGAAATATCTCAAAATTTGCGATGTATGTTGACTTTTTTCCTTGACTGTATAT----TTTCTTGAATT----------------------TCCACAATATTTTGCAACTTATTTTCTTCTTACAGAGACGAAAAATAAGTATCGGGATCAGGATATACAGGAAATCAATTATATG	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
....................................................................................................................................................................................................................................................................TGTTGACTTTTTTCCTTGACTGTATA...........................................................................................................................	26	6	0.50	3	0	3	0	0	0	0
....................................................................................................................................................................................................................................................................TGTTGACTTTTTTCCTTGACTGT..............................................................................................................................	23	15	0.27	4	0	4	0	0	0	0
....................................................................................................................................................................................................................................................................TGTTGACTTTTTTCCTTGACTGTAT............................................................................................................................	25	7	0.14	1	0	1	0	0	0	0
....................................................................................................................................................................................................................................................................TGTTGACTTTTTTCCTTGACTGTA.............................................................................................................................	24	15	0.13	2	0	2	0	0	0	0
.....................................................................................................................................................................................................................................................................GTTGACTTTTTTCCTTGACTGT..............................................................................................................................	22	15	0.13	2	0	2	0	0	0	0
................................................................................................................................................................................................................................................................TGTATGTTGACTTTTTTCCTTGACTG...............................................................................................................................	26	13	0.08	1	0	1	0	0	0	0
....................................................................................................................................................................................................................................................................TGTTGACTTTTTTCCTTGACTG...............................................................................................................................	22	15	0.07	1	0	1	0	0	0	0

dp5

CCACCATCGCCGCAATCTCCAGGACAAAATATACCTTCCTTCCCTCGCGGGTAAGGTGAAACGGCCCAACCAAACGGCTTCTACTTTCGCAATCAGAAATCTCCAAAACCAGGTGTTAACGTTGAACGTCGGCGAAATGTCGAATAATTTATAATTTAATAATCTAATAAATCACTATCCATTAATATCTCAAATAATGGCAAACCAACTTGTTTGATTTTGATTGCAAAACTCCGAAATATCTCAAAATTGGTGCTGTATGTT-ACTCTTTTCCTTGACTGTATAT----TTTCTTGAATTTTCGCTCTGTTTTAAAGCTATTTCAACAATATTATGAAACTTATTTTCTTCTTACAGAGACGAAA--------------TCAG-------AGGAAATCAATTATATG

Size

Hit Count

Total Norm

Total

M022
Male-body

M040
Female-body

M059
Embryo

M062
Head

V043
Embryo

V051
Head

V112
Male-body

SRR902010
Ovary

SRR902011
Testis

SRR902012
CNS imaginal disc

No Reads

droWil2

TAACTTTTG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GTCCTTTAACTTATGACATACTTACATTTTTAATTTGTATTGCAAAAAGCCGAATTATAGCAAAATTTGAGATGTATGTTGACTTTATTCCTTGAGTGTATACTCATTTTTAAAAATTGTTTTTCAATT------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
.................................................................................................................................................................................................................................................................GTATGTTGACTTTATTCCTTGAG.................................................................................................................................	23	1	1.00	1	0	1	0	0	0
...............................................................................................................................................................................................................................................TATAGCAAAATTTGAGATGTATGT..................................................................................................................................................	24	1	1.00	1	0	1	0	0	0
...........................................................................................................................................................................................................................................................TGAGATGTATGTTGACTTTATTCCT.....................................................................................................................................	25	1	1.00	1	0	0	0	1	0

droMoj3

TAATGATTC---------------------------------------------------------------------------------------------------------------------------------------------------------------------CTATGTATTAATGTTCTAACTATTTGCC-----TTATTTTTTATTTTCGTTTCGAAACCCCGAAATATCGAAAAATTGGTGGTATATGTTGACTATTTTCCTTCACTATAAAT--------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M046 Female-body	V041 Embryo	V049 Head	V056 Head	M060 Embryo	V110 Male-body
................................................................................................................................................................................................................................................................TATATGTTGACTATTTTCCTTCACTA...............................................................................................................................	26	1	24.00	24	23	0	0	0	1	0
................................................................................................................................................................................................................................................................TATATGTTGACTATTTTCCTTCACT................................................................................................................................	25	1	20.00	20	14	0	0	0	6	0
...........................................................................................................................................................................................TTCTAACTATTTGCC-----TTATTTTTTATT..............................................................................................................................................................................................	27	1	6.00	6	6	0	0	0	0	0
................................................................................................................................................................................................................................................................TATATGTTGACTATTTTCCTTCACTATA.............................................................................................................................	28	1	5.00	5	3	0	0	0	2	0
................................................................................................................................................................................................................................................................TATATGTTGACTATTTTCCTTCACC................................................................................................................................	25	1	4.00	4	0	0	0	0	4	0
.........................................................................................................................................................................................................................................................TTGGTGGTATATGTTGACTATTTTCC......................................................................................................................................	26	1	4.00	4	3	0	0	0	1	0
................................................................................................................................................................................................................................................................TATATGTTGACTATTTTCCTTCACTAT..............................................................................................................................	27	1	4.00	4	2	0	0	0	2	0
................................................................................................................................................................................................................................................................TATATGTTGACTATTTTCCTTCAC.................................................................................................................................	24	1	3.00	3	1	0	0	0	2	0
................................................................................................................................................................................................................................TTCGAAACCCCGAAATATCGAAAAATTG.............................................................................................................................................................	28	1	1.00	1	1	0	0	0	0	0
.............................................................................................................................................................................................CTAACTATTTGCC-----TTATTTTTTATC..............................................................................................................................................................................................	25	1	1.00	1	1	0	0	0	0	0
.................................................................................................................................................................................................................................TCGAAACCCCGAAATATCGAAAAATTGG............................................................................................................................................................	28	1	1.00	1	1	0	0	0	0	0
.......................................................................................................................................................................................................................TATTTTCGTTTCGAAACCCCGAAATA........................................................................................................................................................................	26	1	1.00	1	0	0	0	0	1	0
...........................................................................................................................................................................................TTCTAACTATTTGCC-----TTATTTTTTATTT.............................................................................................................................................................................................	28	1	1.00	1	1	0	0	0	0	0
................................................................................................................................................................................................................................................................TATATGTTGACTATTTTCCTTCACTATAA............................................................................................................................	29	1	1.00	1	1	0	0	0	0	0
...............................................................................................................................................................................................................................................................GTATATGTTGACTATTTTCCTTCACTA...............................................................................................................................	27	1	1.00	1	1	0	0	0	0	0
............................................................................................................................................................................................TCTAACTATTTGCC-----TTATTTTTTA................................................................................................................................................................................................	24	1	1.00	1	1	0	0	0	0	0
............................................................................................................................................................................................................................TCGTTTCGAAACCCCGAAATATCGAA...................................................................................................................................................................	26	1	1.00	1	1	0	0	0	0	0
..................................................................................................................................................................................................................................CGAAACCCCGAAATATCGAAAAATTGG............................................................................................................................................................	27	1	1.00	1	1	0	0	0	0	0
....................................................................................................................................................................................................................................................................TGTTGACTATTTTCCTTCACTATA.............................................................................................................................	24	1	1.00	1	0	0	0	0	1	0

droRho1

CCACCTCCGCAGAGTCCTCCCGGAT-------ATCCACCTCCC------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droEug1

CCACCTACTCAAAGTTCTCCCGGAT-------ATCTTCCCCCC------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

dpe_1378

scaffold_3:4364671-4364945 -

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-39.1	-38.9	-38.9