dpe_135

TGCAATCAAAAAAAGCATCGCATTCCAGAATCATAAACGATTTATGTAGCTAACGGACAACCTTCTATATTGTTCTGAAAATCCAAAATAGTCGCTTGTCCTTTAGCTGCTGCGATCCACTTGGCAACGATCGAGCACGAACCGTCGACGATCGGTT *********************************...((((...((((((((.((((((....((((.(((...........))).))))....)))))).))))))))...))))....**********************************	Read size	# Mismatch	Hit Count	Total Norm	Total	V111 male body	V057 head	M042 female body	V050 head	M021 embryo	V042 embryo
..................................................TAACGGACAACCTTCTATATTG.....................................................................................	22	0	1	15.00	15	3	4	4	2	2	0
.....................................................................................AAATAGTCGCTTGTCCTTTAG...................................................	21	0	1	10.00	10	6	1	0	3	0	0
.....................................................................................AAATAGTCGCTTGTCCTTTAGC..................................................	22	0	1	3.00	3	0	1	1	0	0	1
..................................................TAACGGACAACCTTCTATATTGA....................................................................................	23	1	1	3.00	3	2	0	0	0	1	0
.....................................................................................AAATAGTCGCTTGTCCTTTAGCT.................................................	23	0	1	3.00	3	2	1	0	0	0	0
...................................................AACGGACAACCTTCTATATTG.....................................................................................	21	0	1	2.00	2	0	0	2	0	0	0
...................................................AACGGACAACCTTCTATATTGT....................................................................................	22	0	1	1.00	1	0	0	0	0	1	0
.....................................................................................AAATAGTCGCTTTTCCTTTAG...................................................	21	1	1	1.00	1	0	1	0	0	0	0
.....................................................................................AAATAGTCGCTTGTCCTTTAGCC.................................................	23	1	1	1.00	1	1	0	0	0	0	0
.....................................................................................AAATAGTCGCTTGTCCTTTCGC..................................................	22	1	1	1.00	1	0	1	0	0	0	0
.....................................................................................AAATAGTCGCTTGTCCATTAG...................................................	21	1	1	1.00	1	1	0	0	0	0	0
.....................................................................................AAATAGCCGCTTGTCCTTTAGC..................................................	22	1	1	1.00	1	0	1	0	0	0	0
TGCAATGTAAATAAGCATCGCA.......................................................................................................................................	22	3	4	0.25	1	0	1	0	0	0	0
........................................................ACAACCTTCTTGATTGTGCTG................................................................................	21	3	5	0.20	1	0	0	0	1	0	0
TGGCATCAAAAGAAGCATCGC........................................................................................................................................	21	3	6	0.17	1	0	1	0	0	0	0

ACGTTAGTTTTTTTCGTAGCGTAAGGTCTTAGTATTTGCTAAATACATCGATTGCCTGTTGGAAGATATAACAAGACTTTTAGGTTTTATCAGCGAACAGGAAATCGACGACGCTAGGTGAACCGTTGCTAGCTCGTGCTTGGCAGCTGCTAGCCAA **********************************...((((...((((((((.((((((....((((.(((...........))).))))....)))))).))))))))...))))....*********************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M021 embryo	V111 male body	V057 head	M042 female body	V042 embryo
....................GTAAGGTCTTATTATTTG.......................................................................................................................	18	1	2	1.00	2	2	0	0	0	0
......................................................................................................................................CGAGCTTGGCAGCTGTTGG....	19	3	15	1.00	15	5	6	2	1	1
....................................................................................................................................CCCGAGCTTGGCAGCTGTT......	19	3	10	0.50	5	3	0	1	1	0
.............................................CATCGATTGCCAGTGGGC..............................................................................................	18	3	17	0.06	1	0	0	1	0	0

Species	Coordinate	ID	Type	Alignment
droPer2	scaffold_13:201807-201963 +	dpe_135	confident	TGCAATCAAAAAAAGCATCGCATTCCAGAATCATAAACGATTTATGTAGCTAACGGACAACCTTCTATATTGTTCTGAAAATCCAAAATAGTCGCTTGTCCTTTAGCTGCTGCGATCCACTTGGCAACGATCG----------------AGCACGAACCGTCGACGATCGGTT
dp5	XL_group1e:8362453-8362625 -	dps-mir-2537	Known_mirbase	TGCAATCAAAAAAAGCATCGCATACCAGAATCATGAACGATTTATGTAGCTAACGGACAACCGTCTATATTGTTCTAAAAATCCAAAATAGTCGCTTGTCCTTTAGCCGCTGCGATCCACTTGCCAACGATCGACTAACAATCCCAGGGAGAACGATCCGTCGACGATCGGTT

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droPer2

TGCAATCAAAAAAAGCATCGCATTCCAGAATCATAAACGATTTATGTAGCTAACGGACAACCTTCTATATTGTTCTGAAAATCCAAAATAGTCGCTTGTCCTTTAGCTGCTGCGATCCACTTGGCAACGATCG----------------AGCACGAACCGTCGACGATCGGTT	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
..................................................TAACGGACAACCTTCTATATTG.....................................................................................................	22	1	15.00	15	2	4	0	2	4	3
.....................................................................................AAATAGTCGCTTGTCCTTTAG...................................................................	21	1	10.00	10	0	0	0	3	1	6
.....................................................................................AAATAGTCGCTTGTCCTTTAGC..................................................................	22	1	3.00	3	0	1	1	0	1	0
..................................................TAACGGACAACCTTCTATATTGA....................................................................................................	23	1	3.00	3	1	0	0	0	0	2
.....................................................................................AAATAGTCGCTTGTCCTTTAGCT.................................................................	23	1	3.00	3	0	0	0	0	1	2
...................................................AACGGACAACCTTCTATATTG.....................................................................................................	21	1	2.00	2	0	2	0	0	0	0
...................................................AACGGACAACCTTCTATATTGT....................................................................................................	22	1	1.00	1	1	0	0	0	0	0
.....................................................................................AAATAGTCGCTTGTCCTTTAGCC.................................................................	23	1	1.00	1	0	0	0	0	0	1

dp5

TGCAATCAAAAAAAGCATCGCATACCAGAATCATGAACGATTTATGTAGCTAACGGACAACCGTCTATATTGTTCTAAAAATCCAAAATAGTCGCTTGTCCTTTAGCCGCTGCGATCCACTTGCCAACGATCGACTAACAATCCCAGGGAGAACGATCCGTCGACGATCGGTT	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
.....................................................................................AAATAGTCGCTTGTCCTTTAGC..................................................................	22	1	401.00	401	0	19	9	15	4	9	9	6	325	5
.....................................................................................AAATAGTCGCTTGTCCTTTAG...................................................................	21	1	326.00	326	1	12	1	1	0	6	13	3	289	0
....................................................................................AAAATAGTCGCTTGTCCTTTAG...................................................................	22	1	76.00	76	1	2	0	0	0	1	0	0	72	0
..................................................TAACGGACAACCGTCTATATTG.....................................................................................................	22	1	76.00	76	0	11	0	0	0	3	3	0	59	0
.....................................................................................AAATAGTCGCTTGTCCTTTAGT..................................................................	22	1	19.00	19	0	0	0	0	0	0	0	0	19	0
.....................................................................................AAATAGTCGCTTGTCCTTTAGA..................................................................	22	1	17.00	17	0	0	0	0	0	0	0	0	17	0
.....................................................................................AAATAGTCGCTTGTCCTTTAGCC.................................................................	23	1	16.00	16	0	0	0	0	0	1	1	0	14	0
....................................................................................AAAATAGTCGCTTGTCCTTTAGC..................................................................	23	1	14.00	14	0	0	0	0	0	1	1	0	12	0
....................................................................................AAAATAGTCGCTTGTCCTTTAGT..................................................................	23	1	14.00	14	0	0	0	0	0	0	0	0	14	0
.....................................................................................AAATAGTCGCTTGTCCTTTAGCT.................................................................	23	1	11.00	11	0	0	1	0	1	0	2	0	7	0
..................................................TAACGGACAACCGTCTATATT......................................................................................................	21	1	10.00	10	0	0	0	0	0	1	0	0	9	0
..................................................TAACGGACAACCGTCTATATTGA....................................................................................................	23	1	9.00	9	0	0	0	0	0	0	0	0	9	0
.....................................................................................AAATAGTCGCTTGTCCTTTAGCA.................................................................	23	1	8.00	8	0	0	0	0	0	1	0	0	7	0
....................................................................................AAAATAGTCGCTTGTCCTTTAGA..................................................................	23	1	8.00	8	0	0	0	0	0	0	0	0	8	0
..................................................TAACGGACAACCGTCTATAT.......................................................................................................	20	1	4.00	4	0	0	0	0	0	2	0	0	2	0
......................................................................................AATAGTCGCTTGTCCTTTAGC..................................................................	21	1	4.00	4	0	4	0	0	0	0	0	0	0	0
.....................................................................................AAATAGTCGCTTGTCCTTTAA...................................................................	21	1	4.00	4	0	0	0	0	0	0	0	0	4	0
.....................................................................................AAATAGTCGCTTGTCCTTTAGTTC................................................................	24	1	4.00	4	0	0	0	0	0	0	0	0	4	0
....................................................................................AAAATAGTCGCTTGTCCTTTA....................................................................	21	1	4.00	4	0	0	0	0	0	1	0	0	3	0
..................................................TAACGGACAACCGTCTATATTGT....................................................................................................	23	1	4.00	4	0	1	0	1	0	0	0	0	2	0
..................................................TAACGGACAACCGTCTATATTGTC...................................................................................................	24	1	3.00	3	0	0	0	0	0	0	0	0	3	0
..................................................TAACGGACAACCGTCTATATTCCC...................................................................................................	24	1	3.00	3	0	0	0	0	0	0	0	0	3	0
.....................................................................................AAATAGTCGCTTGTCCTTTA....................................................................	20	1	3.00	3	0	0	0	0	0	0	1	0	2	0
.....................................................................................AAATAGTCGCTTGTCCTTTAGCCC................................................................	24	1	2.00	2	0	0	0	1	0	0	0	0	1	0
..................................................TAACGGACAACCGTCTATATTGTAA..................................................................................................	25	1	2.00	2	0	0	0	0	0	0	0	0	2	0
..................................................TAACGGACAACCGTCTATATTGTT...................................................................................................	24	1	2.00	2	0	0	0	0	0	0	0	0	2	0
....................................................ACGGACAACCGTCTATATTG.....................................................................................................	20	1	2.00	2	0	0	0	0	0	0	0	0	2	0
..................................................TAACGGACAACCGTCTATATTGAA...................................................................................................	24	1	2.00	2	0	0	0	0	0	0	0	0	2	0
.....................................................................................AAATAGTCGCTTGTCCTTTAGTT.................................................................	23	1	2.00	2	0	0	0	0	0	0	0	0	2	0
.....................................................................................AAATAGTCGCTTGTCCTTTAGTTT................................................................	24	1	2.00	2	0	0	0	0	0	0	0	0	2	0
................................ATGAACGATTTATGTAGC...........................................................................................................................	18	1	1.00	1	0	0	0	0	0	0	0	0	1	0
......................................................................................AATAGTCGCTTGTCCTTTAG...................................................................	20	1	1.00	1	0	0	0	0	0	0	0	0	1	0
..................................................TAACGGACAACCGTCTATATTGTTCT.................................................................................................	26	1	1.00	1	0	0	0	0	0	0	0	0	1	0
....................................................................................AAAATAGTCGCTTGTCCTTTAGG..................................................................	23	1	1.00	1	0	0	0	0	0	0	0	0	1	0
..................................................TAACGGACAACCGTCTATATTGAAA..................................................................................................	25	1	1.00	1	0	0	0	0	0	0	0	0	1	0
...................................................AACGGACAACCGTCTATATTG.....................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	1	0
..................................................TAACGGACAACCGTCTATAA.......................................................................................................	20	1	1.00	1	0	0	0	0	0	0	0	0	1	0
.....................................................................................AAATAGTCGCTTGTCCTTTT....................................................................	20	1	1.00	1	0	0	0	0	0	0	1	0	0	0
...................................................AACGGACAACCGTCTATATTGT....................................................................................................	22	1	1.00	1	0	0	0	0	0	0	0	0	1	0
....................................................................................AAAATAGTCGCTTGTCCTTTTTT..................................................................	23	4	0.25	1	0	0	0	0	0	0	0	0	1	0

dpe_135

scaffold_13:201857-201913 +

Known Ortholog

Testes-restricted

intergenic

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads