dpe_125

CAAGTGATCGAACACAGTTTAATAAAGAAATATTTAAATTTGCATTTCAATTTAGAAACACAAATCATGTGTGGTATGCTTTAACTCATACATAACTTGTGTCTCTTAGATCAAATGTGAGTACACGACGAATTCCCAATTGAGAAGTCCAGTTTTAATTTT *********************************..((((((((((..(((((((.((((((...(((((((((........)).)))))))...)))))).)).))))).))))))))))....**********************************	Read size	# Mismatch	Hit Count	Total Norm	Total	V111 male body	M042 female body	V050 head
........................................................................................TACATAACTTGTGTCTCTTAGAT...................................................	23	0	3	5.33	16	12	3	1
........................................................................................TACATAACTTGTGTCTCTTAG.....................................................	21	0	3	5.00	15	10	4	1
..................................................TTTAGAAACACAAATCATGTGT..........................................................................................	22	0	4	2.50	10	9	1	0
..................................................TTTAGAAACACAAATCATGTGTGT........................................................................................	24	1	4	1.25	5	5	0	0
........................................................................................TACATAACTTGTGTCTCTTAGAA...................................................	23	1	3	1.00	3	3	0	0
........................................................................................TACATAACTTGTGTCTCTTAGA....................................................	22	0	3	0.67	2	1	1	0
........................................................................................TACATAACTTGTGTCTCTTAGATT..................................................	24	1	3	0.67	2	2	0	0
........................................................................................TACATAACTTGTGTCTCTTC......................................................	20	1	3	0.67	2	1	1	0
........................................................................................TACATAACTTGTGTCTCTTAGATCCT................................................	26	2	3	0.67	2	2	0	0
..................................................TTTAGAAACACAAATCATGT............................................................................................	20	0	4	0.50	2	2	0	0
........................................................................................TACATAACTTGTGTCTCTTAGATCA.................................................	25	0	3	0.33	1	1	0	0
........................................................................................TACATAACTTGTGTCTCTTAA.....................................................	21	1	3	0.33	1	1	0	0
........................................................................................TACATAACTTGTGTCTCTTAGAAA..................................................	24	2	3	0.33	1	0	1	0
........................................................................................TACATAACTTGTGTCTCTTAGT....................................................	22	1	3	0.33	1	1	0	0
........................................................................................TACATAACTTGTGTCTCTTAGATC..................................................	24	0	3	0.33	1	1	0	0
..................................................TTTAGAAACACAAATCATGC............................................................................................	20	1	4	0.25	1	1	0	0
...................................................TTAGAAACACAAATCATGTGT..........................................................................................	21	0	4	0.25	1	1	0	0
....................................................TAGAAACACAAATCATGTGTGG........................................................................................	22	0	4	0.25	1	0	1	0
...................................................CTAGAAACACAAATCATGTGTGG........................................................................................	23	1	4	0.25	1	0	1	0
....................................................TAGAAACACAAATCATGTGTGGT.......................................................................................	23	0	4	0.25	1	1	0	0

Species	Coordinate	ID	Alignment
droPer2	scaffold_8585:1083-1244 +	dpe_125	CAAGTGATCGAACACAGTTTAATAAAG-AAATATTTAAATTTGCATTTCAATTTAGAAACACAAATCATGTGTGGTATGCTTTAACTCATACATAACTTGTGTCTCTTAGATCAAATGTGAGTACACGACGAATTCCCAATTGAGAAGTC-CAGTTTTAATTTT
dp5	4_group4:3552997-3553159 +	dps-mir-2566a-2	CAAGTGATCGAAGACAGTTTAATAAAAAAAATATTC-AATTTGCATTTCAATTTAGAAACACAAATCATGTGTGGTATGTTTTAACTCATACATAACTTGTGTCTCTTAGATCAAATGTGAGTACACGACGAATTCCCAATTGAGAAGTCCCAGATTTAAGTTT

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droPer2

CAAGTGATCGAACACAGTTTAATAAAG-AAATATTTAAATTTGCATTTCAATTTAGAAACACAAATCATGTGTGGTATGCTTTAACTCATACATAACTTGTGTCTCTTAGATCAAATGTGAGTACACGACGAATTCCCAATTGAGAAGTC-CAGTTTTAATTTT	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
.........................................................................................TACATAACTTGTGTCTCTTAGAT....................................................	23	3	5.33	16	0	3	0	1	0	12
.........................................................................................TACATAACTTGTGTCTCTTAG......................................................	21	3	5.00	15	0	4	0	1	0	10
...................................................TTTAGAAACACAAATCATGTGT...........................................................................................	22	4	2.50	10	0	1	0	0	0	9
...................................................TTTAGAAACACAAATCATGTGTGT.........................................................................................	24	4	1.25	5	0	0	0	0	0	5
.........................................................................................TACATAACTTGTGTCTCTTAGAA....................................................	23	3	1.00	3	0	0	0	0	0	3
.........................................................................................TACATAACTTGTGTCTCTTAGA.....................................................	22	3	0.67	2	0	1	0	0	0	1
.........................................................................................TACATAACTTGTGTCTCTTAGATT...................................................	24	3	0.67	2	0	0	0	0	0	2
.........................................................................................TACATAACTTGTGTCTCTTC.......................................................	20	3	0.67	2	0	1	0	0	0	1
.........................................................................................TACATAACTTGTGTCTCTTAGATCCT.................................................	26	3	0.67	2	0	0	0	0	0	2
...................................................TTTAGAAACACAAATCATGT.............................................................................................	20	4	0.50	2	0	0	0	0	0	2
.........................................................................................TACATAACTTGTGTCTCTTAGATCA..................................................	25	3	0.33	1	0	0	0	0	0	1
.........................................................................................TACATAACTTGTGTCTCTTAA......................................................	21	3	0.33	1	0	0	0	0	0	1
.........................................................................................TACATAACTTGTGTCTCTTAGAAA...................................................	24	3	0.33	1	0	1	0	0	0	0
.........................................................................................TACATAACTTGTGTCTCTTAGT.....................................................	22	3	0.33	1	0	0	0	0	0	1
.........................................................................................TACATAACTTGTGTCTCTTAGATC...................................................	24	3	0.33	1	0	0	0	0	0	1
...................................................TTTAGAAACACAAATCATGC.............................................................................................	20	4	0.25	1	0	0	0	0	0	1
....................................................TTAGAAACACAAATCATGTGT...........................................................................................	21	4	0.25	1	0	0	0	0	0	1
.....................................................TAGAAACACAAATCATGTGTGG.........................................................................................	22	4	0.25	1	0	1	0	0	0	0
.....................................................TAGAAACACAAATCATGTGTGGT........................................................................................	23	4	0.25	1	0	0	0	0	0	1

dp5

CAAGTGATCGAAGACAGTTTAATAAAAAAAATATTC-AATTTGCATTTCAATTTAGAAACACAAATCATGTGTGGTATGTTTTAACTCATACATAACTTGTGTCTCTTAGATCAAATGTGAGTACACGACGAATTCCCAATTGAGAAGTCCCAGATTTAAGTTT	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
.........................................................................................TACATAACTTGTGTCTCTTAGA.....................................................	22	2	369.50	739	0	26	0	0	0	0	4	0	707	2
.........................................................................................TACATAACTTGTGTCTCTTAG......................................................	21	2	277.00	554	0	21	0	0	0	5	16	4	508	0
.........................................................................................TACATAACTTGTGTCTCTTAGAT....................................................	23	2	99.50	199	1	15	2	2	6	3	19	2	149	0
.........................................................................................TACATAACTTGTGTCTCTTAGAA....................................................	23	2	75.50	151	0	1	0	0	0	1	3	0	146	0
.....................................................TAGAAACACAAATCATGTGTGG.........................................................................................	22	1	37.00	37	0	4	0	0	1	0	1	0	31	0
.........................................................................................TACATAACTTGTGTCTCTTAGATC...................................................	24	2	32.00	64	1	1	0	0	0	0	0	0	62	0
...................................................TTTAGAAACACAAATCATGTGTG..........................................................................................	23	1	20.00	20	0	0	0	0	0	0	0	0	20	0
...................................................TTTAGAAACACAAATCATGTGT...........................................................................................	22	1	18.00	18	0	0	0	0	0	1	7	0	10	0
.........................................................................................TACATAACTTGTGTCTCTTA.......................................................	20	2	17.00	34	0	2	0	1	0	0	0	0	31	0
.........................................................................................TACATAACTTGTGTCTCTTAGATCC..................................................	25	2	17.00	34	0	2	0	1	0	0	1	0	30	0
.........................................................................................TACATAACTTGTGTCTCTTAGAAA...................................................	24	2	16.00	32	0	2	0	0	0	0	0	0	30	0
.........................................................................................TACATAACTTGTGTCTCTTAGATT...................................................	24	2	14.50	29	0	3	0	0	0	1	1	0	24	0
.........................................................................................TACATAACTTGTGTCTCTTAGATCCC.................................................	26	2	9.00	18	1	0	0	0	0	0	1	0	16	0
.........................................................................................TACATAACTTGTGTCTCTTAGATA...................................................	24	2	8.50	17	0	2	0	1	0	0	0	0	14	0
...................................................TTTAGAAACACAAATCATGTGTGGTA.......................................................................................	26	1	6.00	6	0	0	0	0	0	0	0	0	6	0
...................................................TTTAGAAACACAAATCATGTG............................................................................................	21	1	6.00	6	0	1	0	0	0	0	0	0	5	0
.........................................................................................TACATAACTTGTGTCTCTTAA......................................................	21	2	4.50	9	0	0	0	0	0	1	0	0	8	0
.........................................................................................TACATAACTTGTGTCTCTTAGATTT..................................................	25	2	4.00	8	0	1	0	0	0	0	0	0	7	0
...................................................TTTAGAAACACAAATCATGTGTGT.........................................................................................	24	1	4.00	4	0	1	0	0	0	0	0	0	3	0
........................................................................................ATACATAACTTGTGTCTCTTAGA.....................................................	23	2	3.50	7	0	0	0	0	0	0	0	0	7	0
.........................................................................................TACATAACTTGTGTCTCTTT.......................................................	20	2	3.00	6	0	0	0	0	0	0	0	0	6	0
...................................................TTTAGAAACACAAATCATGTGTGGT........................................................................................	25	1	3.00	3	0	1	0	0	0	0	1	0	1	0
...................................................TTTAGAAACACAAATCATGTGTGG.........................................................................................	24	1	3.00	3	0	1	0	0	0	0	0	0	2	0
.........................................................................................TACATAACTTGTGTCTCTTAGT.....................................................	22	2	2.50	5	0	0	0	0	0	0	1	0	4	0
...................................................TTTAGAAACACAAATCATGTGTA..........................................................................................	23	1	2.00	2	0	0	0	0	0	0	0	0	2	0
....................................................TTAGAAACACAAATCATGTGTG..........................................................................................	22	1	2.00	2	0	0	0	0	0	0	0	0	2	0
.........................................................................................TACATAACTTGTGTCTCTTAGATCA..................................................	25	2	2.00	4	0	0	0	0	0	0	0	0	4	0
.....................................................TAGAAACACAAATCATGTGT...........................................................................................	20	1	2.00	2	0	0	0	0	0	0	2	0	0	0
.............................................................................TGTTTTAACTCATACATAACTTGTGTC............................................................	27	1	2.00	2	0	0	0	0	0	0	0	0	2	0
.........................................................................................TACATAACTTGTGTCTCTTAGATCT..................................................	25	2	1.50	3	0	0	0	0	0	0	0	0	3	0
.........................................................................................TACATAACTTGTGTCTCTTAGTCT...................................................	24	2	1.50	3	0	0	0	0	0	0	0	0	3	0
.........................................................................................TACATAACTTGTGTCTCTTAGATTCC.................................................	26	2	1.50	3	0	1	0	0	0	0	0	0	2	0
..........................................................................................ACATAACTTGTGTCTCTTAGA.....................................................	21	2	1.50	3	0	0	0	0	0	0	0	0	3	0
.........................................................................................TACATAACTTGTGTCTCTTAGATCCCC................................................	27	2	1.00	2	0	0	0	0	0	0	0	0	2	0
..................................................................................TAACTCATACATAACTTGTGTCTCT.........................................................	25	2	1.00	2	0	0	0	0	0	0	0	0	2	0
...................................................TTTAGAAACACAAATCATGT.............................................................................................	20	1	1.00	1	0	1	0	0	0	0	0	0	0	0
...................................................TTTAGAAACACAAATCATGTGTGA.........................................................................................	24	1	1.00	1	0	0	0	0	0	0	0	0	1	0
.....................................................TAGAAACACAAATCATGTGTGGTA.......................................................................................	24	1	1.00	1	0	0	0	0	0	0	0	0	1	0
.....................................................TAGAAACACAAATCATGTGTG..........................................................................................	21	1	1.00	1	0	0	0	0	0	0	1	0	0	0
..................................................................................TAACTCATACATAACTTGTGTCTCTTAGA.....................................................	29	2	1.00	2	0	0	0	0	0	0	0	0	2	0
...................................................TTTAGAAACACAAATCATG..............................................................................................	19	1	1.00	1	0	0	0	0	0	0	0	0	1	0
.........................................................................................TACATAACTTGTGTCTCTTG.......................................................	20	2	1.00	2	0	0	0	0	0	0	0	0	2	0
.........................................................................................TACATAACTTGTGTCTCTTAGATCAA.................................................	26	2	1.00	2	0	0	0	0	0	0	0	0	2	0
..........................................................................................ACATAACTTGTGTCTCTTAG......................................................	20	2	1.00	2	0	2	0	0	0	0	0	0	0	0
.....................................................TAGAAACACAAATCATGTGTGT.........................................................................................	22	1	1.00	1	0	0	0	0	0	0	1	0	0	0
..........................................................................................ACATAACTTGTGTCTCTTAGAT....................................................	22	2	1.00	2	0	2	0	0	0	0	0	0	0	0
..................................................................................TAACTCATACATAACTTGTGTCT...........................................................	23	2	0.50	1	0	0	0	0	0	0	1	0	0	0
............................................................................................ATAACTTGTGTCTCTTAGA.....................................................	19	2	0.50	1	0	1	0	0	0	0	0	0	0	0
................................................................................TTTAACTCATACATAACTTGTGTCTC..........................................................	26	2	0.50	1	0	0	0	0	0	0	0	0	1	0
.........................................................................................TACATAACTTGTGTCTCTTAGATCG..................................................	25	2	0.50	1	0	1	0	0	0	0	0	0	0	0
.........................................................................................TACATAACTTGTGTCTCTTAGAC....................................................	23	2	0.50	1	0	0	0	1	0	0	0	0	0	0
.........................................................................................TACATAACTTGTGTCTCTTAGATAT..................................................	25	2	0.50	1	0	0	0	0	0	0	0	0	1	0
..........................................................................................ACATAACTTGTGTCTCTTAGATA...................................................	23	2	0.50	1	0	1	0	0	0	0	0	0	0	0
........................................................................................ATACATAACTTGTGTCTCTTAG......................................................	22	2	0.50	1	0	0	0	0	0	0	0	0	1	0
.........................................................................................TACATAACTTGTGTCTCTTAGATCCCT................................................	27	2	0.50	1	0	0	0	0	0	0	1	0	0	0
.........................................................................................TACATAACTTGTGTCTCTTAGATG...................................................	24	2	0.50	1	0	0	0	0	0	1	0	0	0	0
..................................................................................TAACTCATACATAACTTGTGTCTCTTA.......................................................	27	2	0.50	1	0	0	0	0	0	0	0	0	1	0
.........................................................................................TACATAACTTGTGTCTCTTAGG.....................................................	22	2	0.50	1	0	0	0	0	0	0	0	0	1	0
.........................................................................................TACATAACTTGTGTCTCTTAGATCCT.................................................	26	2	0.50	1	0	0	0	0	0	0	0	0	1	0
.......................................................................................CATACATAACTTGTGTCTCTTAGAT....................................................	25	2	0.50	1	0	0	0	0	0	0	0	0	1	0
................................................................................TTTAACTCATACATAACTTGTGTCTCT.........................................................	27	2	0.50	1	0	0	0	0	0	0	0	0	1	0
..................................................................................................TGTGTCTCTTAGATCT..................................................	16	6	0.17	1	0	0	0	0	0	0	0	0	1	0

dpe_125

scaffold_8585:1133-1194 +

Known Ortholog

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-22.9	-22.6