dpe_120

CACGAACCGTCGACGATCGGTTATGTTTTCTAGAGAAGAACTGGCAGTGCAATACGTTTTTCTTCGCACTGATGTGTGTTTAAACTCCAATGCGTTGAATAACGTTTTACAGTGTGCGTTTCTGAGCATCGCCGTGTAGGTGAGACTGTGCCGTGCAAC *********************************..((((..(((.((.((.(((((.(((..((((.((..((........))..)).))))..))).))))).)).)).)))..))))..**********************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M021 embryo	M042 female body	V057 head	V111 male body	V050 head	V042 embryo
..................................................AATACGTTTTTCTTCGCACTGA.......................................................................................	22	0	1	63.00	63	41	16	4	2	0	0
..................................................AATACGTTTTTCTTCGCACTG........................................................................................	21	0	1	18.00	18	3	12	0	2	0	1
..................................................AATACGTTTTTCTTCGCACTGG.......................................................................................	22	1	1	5.00	5	5	0	0	0	0	0
...................................................ATACGTTTTTCTTCGCACTGA.......................................................................................	21	0	1	3.00	3	0	3	0	0	0	0
........................................................................................AATGCGTTGAATAACGTTTT...................................................	20	0	1	2.00	2	0	0	2	0	0	0
..................................................AATACGTTTTTCTTCGCATTG........................................................................................	21	1	1	1.00	1	0	0	0	0	1	0
........................................................................................AATGCGTTGAATAACGTTTTA..................................................	21	0	1	1.00	1	0	0	0	0	1	0
.............................CTAGAGAAGAACTGGCAGTGC.............................................................................................................	21	0	1	1.00	1	0	0	0	1	0	0
..................................................AATACGTTTTTCTTAGCACTG........................................................................................	21	1	1	1.00	1	0	1	0	0	0	0
........................................................................................AATGCGATGAGTAACGTTCTAC.................................................	22	3	1	1.00	1	0	0	1	0	0	0
........................................................................................AATGCGTTGAATAACGTTTTAC.................................................	22	0	1	1.00	1	0	0	0	0	1	0
..................................................AATACGTTTTTCTTCGCACT.........................................................................................	20	0	1	1.00	1	0	1	0	0	0	0

Species	Coordinate	ID	Type	Alignment
droPer2	scaffold_13:201942-202100 +	dpe_120	confident	CACGAACCGTCGACGATCGGTTATGTTTTCTAGAGAAGAACTGGCAGTGCAATACGTTTTTCTTCGCACTGATGTGTGTTTAAACTCCAATGCGTTGAATAACGTTTTACAGTGTGCGTTTCTGAGCATCGCCGTGTAGGTGAGACTGTGCCGTGCAAC
dp5	XL_group1e:8362316-8362474 -	dps-mir-2536	Known_mirbase	AACGATCCGTCGACGATCGGTTGTGATTTCTAGAGAAGAACTGGCAGTGCAATACGTTTTTCTTCGCACTGATGTGTGTTTAAACCTCAATGCGTTGAATAACGTTTTACAGTGTGCGTTTCTGAGCATCGCCATGTGGCTGAAACTGTGCCGTGCAAC

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droPer2

CACGAACCGTCGACGATCGGTTATGTTTTCTAGAGAAGAACTGGCAGTGCAATACGTTTTTCTTCGCACTGATGTGTGTTTAAACTCCAATGCGTTGAATAACGTTTTACAGTGTGCGTTTCTGAGCATCGCCGTGTAGGTGAGACTGTGCCGTGCAAC	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
..................................................AATACGTTTTTCTTCGCACTGA.......................................................................................	22	1	63.00	63	41	16	0	0	4	2
..................................................AATACGTTTTTCTTCGCACTG........................................................................................	21	1	18.00	18	3	12	1	0	0	2
..................................................AATACGTTTTTCTTCGCACTGG.......................................................................................	22	1	5.00	5	5	0	0	0	0	0
...................................................ATACGTTTTTCTTCGCACTGA.......................................................................................	21	1	3.00	3	0	3	0	0	0	0
........................................................................................AATGCGTTGAATAACGTTTT...................................................	20	1	2.00	2	0	0	0	0	2	0
........................................................................................AATGCGTTGAATAACGTTTTA..................................................	21	1	1.00	1	0	0	0	1	0	0
.............................CTAGAGAAGAACTGGCAGTGC.............................................................................................................	21	1	1.00	1	0	0	0	0	0	1
........................................................................................AATGCGTTGAATAACGTTTTAC.................................................	22	1	1.00	1	0	0	0	1	0	0
..................................................AATACGTTTTTCTTCGCACT.........................................................................................	20	1	1.00	1	0	1	0	0	0	0

dp5

AACGATCCGTCGACGATCGGTTGTGATTTCTAGAGAAGAACTGGCAGTGCAATACGTTTTTCTTCGCACTGATGTGTGTTTAAACCTCAATGCGTTGAATAACGTTTTACAGTGTGCGTTTCTGAGCATCGCCATGTGGCTGAAACTGTGCCGTGCAAC	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
..................................................AATACGTTTTTCTTCGCACTG........................................................................................	21	1	710.00	710	18	26	1	4	1	1	4	1	654	0
..................................................AATACGTTTTTCTTCGCACTGA.......................................................................................	22	1	543.00	543	31	69	8	7	1	1	4	0	419	3
..................................................AATACGTTTTTCTTCGCACTGT.......................................................................................	22	1	74.00	74	0	0	0	0	0	0	1	0	73	0
..................................................AATACGTTTTTCTTCGCACTGAA......................................................................................	23	1	40.00	40	0	2	0	0	0	1	0	0	37	0
..................................................AATACGTTTTTCTTCGCACTGAAA.....................................................................................	24	1	36.00	36	0	2	0	1	0	0	0	0	33	0
...................................................ATACGTTTTTCTTCGCACTG........................................................................................	20	1	18.00	18	0	4	0	0	0	0	0	0	14	0
...................................................ATACGTTTTTCTTCGCACTGAT......................................................................................	22	1	15.00	15	0	0	0	0	0	0	0	0	15	0
........................................................................................AATGCGTTGAATAACGTTTTAC.................................................	22	1	14.00	14	0	0	0	0	0	2	2	0	10	0
..................................................AATACGTTTTTCTTCGCACTGAT......................................................................................	23	1	14.00	14	0	0	0	0	0	0	0	0	14	0
...................................................ATACGTTTTTCTTCGCACTGA.......................................................................................	21	1	14.00	14	1	1	0	0	0	0	0	0	12	0
..................................................AATACGTTTTTCTTCGCAC..........................................................................................	19	1	12.00	12	4	0	1	4	0	0	0	0	3	0
..................................................AATACGTTTTTCTTCGCACTGTC......................................................................................	23	1	12.00	12	0	0	0	0	0	0	0	0	12	0
..................................................AATACGTTTTTCTTCGCACT.........................................................................................	20	1	10.00	10	0	0	0	0	0	0	0	0	10	0
....................................................TACGTTTTTCTTCGCACTGATG.....................................................................................	22	1	10.00	10	0	0	0	0	0	0	0	0	10	0
........................................................................................AATGCGTTGAATAACGTTTTA..................................................	21	1	9.00	9	0	0	0	0	0	0	1	0	8	0
..............................TAGAGAAGAACTGGCAGTGC.............................................................................................................	20	1	7.00	7	0	1	0	0	0	0	1	0	5	0
..................................................AATACGTTTTTCTTCGCACTGATG.....................................................................................	24	1	7.00	7	0	0	0	0	0	0	0	0	7	0
..................................................AATACGTTTTTCTTCGCACTGAAAA....................................................................................	25	1	5.00	5	0	0	0	0	0	0	0	0	5	0
...................................................ATACGTTTTTCTTCGCACTGATC.....................................................................................	23	1	4.00	4	0	0	0	0	0	0	0	0	4	0
..................................................AATACGTTTTTCTTCGCACTCT.......................................................................................	22	1	4.00	4	0	0	0	0	0	0	0	0	4	0
......................................................CGTTTTTCTTCGCACTGA.......................................................................................	18	1	4.00	4	0	1	0	0	0	0	0	0	3	0
..................................................AATACGTTTTTCTTCGCACTGC.......................................................................................	22	1	4.00	4	0	0	0	0	0	0	0	0	4	0
..................................................AATACGTTTTTCTTCGCACTGAG......................................................................................	23	1	4.00	4	0	0	0	0	0	0	0	0	4	0
..................................................AATACGTTTTTCTTCGCACTGATT.....................................................................................	24	1	4.00	4	0	0	0	0	0	0	0	0	4	0
..................................................AATACGTTTTTCTTCGCACTGACA.....................................................................................	24	1	3.00	3	0	0	0	0	0	0	0	0	3	0
......................................................CGTTTTTCTTCGCACTG........................................................................................	17	1	3.00	3	0	0	0	0	0	0	0	0	3	0
..................................................AATACGTTTTTCTTCGCACTT........................................................................................	21	1	3.00	3	0	0	0	0	0	0	0	0	3	0
...................................................ATACGTTTTTCTTCGCACTGTC......................................................................................	22	1	2.00	2	0	0	0	0	0	0	0	0	2	0
..................................................AATACGTTTTTCTTCGCACTCC.......................................................................................	22	1	2.00	2	0	0	0	0	0	0	0	0	2	0
...................................................ATACGTTTTTCTTCGCACTGT.......................................................................................	21	1	2.00	2	0	1	0	0	0	0	0	0	1	0
................................GAGAAGAACTGGCAGTGC.............................................................................................................	18	1	2.00	2	0	0	0	0	0	0	0	0	2	0
...................................................ATACGTTTTTCTTCGCACTGATA.....................................................................................	23	1	2.00	2	0	0	0	0	0	0	0	0	2	0
....................................................TACGTTTTTCTTCGCACTGA.......................................................................................	20	1	2.00	2	0	0	0	0	0	0	0	0	2	0
..................................................AATACGTTTTTCTTCGCACTGTCA.....................................................................................	24	1	2.00	2	0	0	0	0	0	0	0	0	2	0
..................................................AATACGTTTTTCTTCGCA...........................................................................................	18	1	2.00	2	0	0	0	0	0	0	2	0	0	0
.............................CTAGAGAAGAACTGGCAGTGC.............................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	1	0
..................................................AATACGTTTTTCTTCGCACTC........................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	1	0
........................................................................................AATGCGTTGAATAACGTTTTACCC...............................................	24	1	1.00	1	0	0	0	1	0	0	0	0	0	0
........................................................TTTTTCTTCGCACTGA.......................................................................................	16	1	1.00	1	0	0	0	0	0	0	0	0	1	0
...................................................ATACGTTTTTCTTCGCACTGATG.....................................................................................	23	1	1.00	1	0	0	0	0	0	0	0	0	1	0
..................................................AATACGTTTTTCTTCGCACTGTCC.....................................................................................	24	1	1.00	1	0	0	0	0	0	0	0	0	1	0
...............................AGAGAAGAACTGGCAGTGC.............................................................................................................	19	1	1.00	1	0	1	0	0	0	0	0	0	0	0
...................................................ATACGTTTTTCTTCGCACTGATT.....................................................................................	23	1	1.00	1	0	0	0	0	0	0	0	0	1	0
..................................................AATACGTTTTTCTTCGCACTGTA......................................................................................	23	1	1.00	1	0	0	0	0	0	0	0	0	1	0
.........................................................................................ATGCGTTGAATAACGTTTTACA................................................	22	1	1.00	1	0	0	0	0	0	0	0	0	1	0
..................................................AATACGTTTTTCTTCGCACCC........................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	1	0
..................................................AATACGTTTTTCTTCGCACTGATA.....................................................................................	24	1	1.00	1	0	0	0	0	0	0	0	0	1	0
...................................................ATACGTTTTTCTTCGCACTGAG......................................................................................	22	1	1.00	1	0	0	0	0	0	0	0	0	1	0
..................................................AATACGTTTTTCTTCGCACC.........................................................................................	20	1	1.00	1	0	0	0	1	0	0	0	0	0	0
.....................................................ACGTTTTTCTTCGCACTGATG.....................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	1	0
.......................................................GTTTTTCTTCGCACTGA.......................................................................................	17	1	1.00	1	0	0	0	0	0	0	0	0	1	0
....................................................TACGTTTTTCTTCGCACTGAT......................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	1	0
..................................................AATACGTTTTTCTTCGCACTGTAA.....................................................................................	24	3	0.67	2	0	0	0	0	0	0	0	0	2	0
......................................................CGTTTTTCTTCGCACTGAAA.....................................................................................	20	3	0.67	2	0	0	0	0	0	0	0	0	2	0

dpe_120

scaffold_13:201992-202050 +

Known Ortholog

Testes-restricted

intergenic

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-25.2	-25.2