dpe_1098

exon [dper_GLEANR_10608:1]; CDS [Dper\GL10481-cds]; exon [dper_GLEANR_10608:2]; CDS [Dper\GL10481-cds]; intron [Dper\GL10481-in]

Sense Strand Reads

-----------------------------#####################----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------################################################## AAAGTACAGGAACCGCCGGGGAGTTTACGATGGCCGCCGATCAGGCGAGGGTATCAAAAAATAAAAGAAGAAGACCTAAGAAAAAAATAGTCAATGCAAAAGATGAATAGGGAGGAAACAAGGAAAACGTACCCGGTTTGGGCTGGCTCATGATGACGCGGTGGTAGCCGAACGCCAGAGCCGAATACCCTGGAGTCTAATCCAGAATCCATGTACGAACTGGCATATCTATATATGTAGCTCTATAAGCTAATCGTGATCTACAAGACTCAGAGCTATCGCAACGCGGG ************************************************............................................((((..(((((..(((.......(((.(((....(((..((((((.((((((((.((.((......)).)))))))...))).))))))..))).(((((......)))))..))).))).....)))...))))).....)))).************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M042 female body	V111 male body	V057 head	M021 embryo
...................................................................................................................................................................................................TCTAAGTCAGAATCCATG.............................................................................	18	2	3	0.33	1	1	0	0	0
..................................................................................................................................................................................................CTCTAAGTCAGAATCCATG.............................................................................	19	3	19	0.26	5	2	1	0	2
............................................................................................................................................GGCTGGCTCATGATGACGCGGT................................................................................................................................	22	0	6	0.17	1	0	1	0	0
.......................................................................................................................................................................................AATACCCGGGAGTATAAGC........................................................................................	19	3	10	0.10	1	0	0	1	0
.....................................................................................................................................................................................................................................................................TACAACACTCAGAAATATCGC........	21	3	14	0.07	1	0	0	1	0

-----------------------------#####################----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------##################################################
AAAGTACAGGAACCGCCGGGGAGTTTACGATGGCCGCCGATCAGGCGAGGGTATCAAAAAATAAAAGAAGAAGACCTAAGAAAAAAATAGTCAATGCAAAAGATGAATAGGGAGGAAACAAGGAAAACGTACCCGGTTTGGGCTGGCTCATGATGACGCGGTGGTAGCCGAACGCCAGAGCCGAATACCCTGGAGTCTAATCCAGAATCCATGTACGAACTGGCATATCTATATATGTAGCTCTATAAGCTAATCGTGATCTACAAGACTCAGAGCTATCGCAACGCGGG
**************************************************............................................((((..(((((..(((.......(((.(((....(((..((((((.((((((((.((.((......)).)))))))...))).))))))..))).(((((......)))))..))).))).....)))...))))).....)))).**************************************************

Read size

# Mismatch

Hit Count

Total Norm

Total

M042
female body

V111
male body

V057
head

M021
embryo

...................................................................................................................................................................................................TCTAAGTCAGAATCCATG.............................................................................

0.33

..................................................................................................................................................................................................CTCTAAGTCAGAATCCATG.............................................................................

0.26

............................................................................................................................................GGCTGGCTCATGATGACGCGGT................................................................................................................................

0.17

.......................................................................................................................................................................................AATACCCGGGAGTATAAGC........................................................................................

0.10

.....................................................................................................................................................................................................................................................................TACAACACTCAGAAATATCGC........

0.07

Anti-sense strand reads

Re-alignment of all predicted orthologs

Species	Coordinate	ID	Alignment
droPer2	scaffold_2:7190379-7190668 -	dpe_1098	AAAGTACAGGAACCGCCGGGGAGTTTACGATGGCCGCCGATCAGGCGAGGGTATCAAAAAATAAAAGAAGAAGACCTAAGAAAAAAATAGTCAATGCAAAAGATGAATAGGGAGGAAACAAGGAAAACGTACCCGGTTTGGGCTGGCTCATGATGACGCGGTGGTAGCCGAACGCCAGAGCCGAATACCCTGGAGTCTAATCCAGAATCCATGTACGAACTGGCATATCTATATATGTAGCTCTATAAGCTAATCGTGATCTACAAGACTCAGAGCTATCGCAACGCGGG
dp5	3:6989224-6989505 -	dps_1065	AAAGTACAGGAACCGCCGGGGAGTTTACGATGGCCGCCGATCAGGCGAGGGTATCAAAAAATAAAAGAAGAAGACCTAAGAAAAAAATAGTCAATGCAAAAGATGAATAGGGAGGAAACAAGGAAAACGTACCCGGTTTGGGCTGGCTCATGATGACGCGGTGGTAGCCGAACGCCAGAGCCGAATACCCTGGAGTCTAATCCAGAATCCATGTACGAACTGGCAT--------ATGTAGCTCTATAAGCTAATCGTGATCTACAAGACTCAGAGCTATCGCAACGCGGG

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droPer2

AAAGTACAGGAACCGCCGGGGAGTTTACGATGGCCGCCGATCAGGCGAGGGTATCAAAAAATAAAAGAAGAAGACCTAAGAAAAAAATAGTCAATGCAAAAGATGAATAGGGAGGAAACAAGGAAAACGTACCCGGTTTGGGCTGGCTCATGATGACGCGGTGGTAGCCGAACGCCAGAGCCGAATACCCTGGAGTCTAATCCAGAATCCATGTACGAACTGGCATATCTATATATGTAGCTCTATAAGCTAATCGTGATCTACAAGACTCAGAGCTATCGCAACGCGGG	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
............................................................................................................................................GGCTGGCTCATGATGACGCGGT................................................................................................................................	22	6	0.17	1	0	0	0	0	0	1

dp5

AAAGTACAGGAACCGCCGGGGAGTTTACGATGGCCGCCGATCAGGCGAGGGTATCAAAAAATAAAAGAAGAAGACCTAAGAAAAAAATAGTCAATGCAAAAGATGAATAGGGAGGAAACAAGGAAAACGTACCCGGTTTGGGCTGGCTCATGATGACGCGGTGGTAGCCGAACGCCAGAGCCGAATACCCTGGAGTCTAATCCAGAATCCATGTACGAACTGGCAT--------ATGTAGCTCTATAAGCTAATCGTGATCTACAAGACTCAGAGCTATCGCAACGCGGG	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
.............................................................................................................................................GCTGGCTCATGATGACGCGG.................................................................................................................................	20	7	0.14	1	0	1	0	0	0	0	0	0	0	0
............................................................................................................................................GGCTGGCTCATGATGACGCG..................................................................................................................................	20	8	0.13	1	0	1	0	0	0	0	0	0	0	0
..................................................................................................................................................................GGTAGCCGAACGCCAGAGC.............................................................................................................	19	9	0.11	1	0	1	0	0	0	0	0	0	0	0

dpe_1098

scaffold_2:7190429-7190618 -

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-34.8	-34.7	-34.7