dpe_109

CTTATCAGATCCGCAATGCTTCCCGATCACCATGAATTTCCACTCGAAAATATGATATGTTCGGCATCCTCCGGGCACAATGTGAAGGAGGATGCCAAGCATATTATATTTTCTTGTGGTAGATTCTCAGCGCAGCGCCACCGGCTGGAGGAAGCCATGG *********************************((((((((..((((((((((((((((.((((((((((...(((...)))..)))))))))).))))))))))))))))..)))).)))).*********************************	Read size	# Mismatch	Hit Count	Total Norm	Total	V057 head	M042 female body	M021 embryo	V050 head	V111 male body
..................................................TATGATATGTTCGGCATCCTC.........................................................................................	21	0	1	31.00	31	8	6	15	2	0
..................................................TATGATATGTTCGGCATCCTCC........................................................................................	22	0	1	31.00	31	10	9	1	6	5
...................................................ATGATATGTTCGGCATCCTC.........................................................................................	20	0	1	18.00	18	12	3	1	2	0
...................................................ATGATATGTTCGGCATCCTCC........................................................................................	21	0	1	16.00	16	5	8	0	3	0
..................................................TATGATATGTTCGGCATCCT..........................................................................................	20	0	1	6.00	6	1	1	2	1	1
.................................................ATATGATATGTTCGGCATCCT..........................................................................................	21	0	1	3.00	3	1	1	0	1	0
...................................................GTGATATGTTCGGCATCCTC.........................................................................................	20	1	1	3.00	3	2	0	0	0	1
..................................................TATGATATGTTCGGCATCC...........................................................................................	19	0	1	3.00	3	2	1	0	0	0
..................................................TGTGATATGTTCGGCATCCTCC........................................................................................	22	1	1	3.00	3	2	1	0	0	0
........................................................................................AGGATGCCAAGCATATTATATT..................................................	22	0	1	2.00	2	0	2	0	0	0
........................................................................................AGGATGCCAAGCATATTATAT...................................................	21	0	1	2.00	2	0	1	0	1	0
..................................................TATGATATGTTAGGCATCCTC.........................................................................................	21	1	1	1.00	1	1	0	0	0	0
..................................................TATGATATGTTCTACATCCT..........................................................................................	20	2	1	1.00	1	0	0	0	1	0
..................................................TATGATATGTTCGGCATCCTCA........................................................................................	22	1	1	1.00	1	0	1	0	0	0
..................................................TATGATATGTTCGGCATCCTCCT.......................................................................................	23	1	3	1.00	3	3	0	0	0	0
..................................................TATGATATGTTCGGCATCCTCCA.......................................................................................	23	1	1	1.00	1	0	1	0	0	0
........................................................................................AGGATGCCAAGCATATTATA....................................................	20	0	1	1.00	1	0	1	0	0	0
..................................................TATGATATGTTGGGCATCTTC.........................................................................................	21	2	1	1.00	1	1	0	0	0	0
..................................................TATGGTATGTTCGGCATCCTC.........................................................................................	21	1	1	1.00	1	1	0	0	0	0
..................................................TATGAGATGTTCGGCATCCT..........................................................................................	20	1	1	1.00	1	1	0	0	0	0
..................................................TATGATATATTCGGCATCCTC.........................................................................................	21	1	1	1.00	1	0	1	0	0	0
.................................................ATATGATATGTTCGGCATCCTC.........................................................................................	22	0	1	1.00	1	0	0	1	0	0
..................................................................................TGAAGGAGGATGCCAAGCAT..........................................................	20	0	1	1.00	1	0	0	0	0	1
..................................................TATGATATGTTCTGCATCCTCC........................................................................................	22	1	1	1.00	1	1	0	0	0	0
.............................................................................CAATGTGAAGGAGGATGCCAAGC............................................................	23	0	1	1.00	1	0	0	0	0	1
...................................................ATGATATGTTCGGCATCCTCCA.......................................................................................	22	1	1	1.00	1	1	0	0	0	0
..................................................TATGATATGTTCGGTATCCTCC........................................................................................	22	1	1	1.00	1	0	0	0	1	0
...................................................GTGATATGTTCGGCATCCTCC........................................................................................	21	1	1	1.00	1	1	0	0	0	0
............................................................................ACAATGTGAAGGAGGATGCCAAG.............................................................	23	0	1	1.00	1	0	1	0	0	0
...................................................ATGATATGGTCGGCATCCTC.........................................................................................	20	1	2	0.50	1	1	0	0	0	0
..................................................TATGAGATGGTCGGCATCGTC.........................................................................................	21	3	3	0.33	1	1	0	0	0	0
.......................................CCACTAGGAAATATGATAT......................................................................................................	19	2	3	0.33	1	0	1	0	0	0
............................................................................ACAATGCCAAGTAGGATGCC................................................................	20	3	20	0.10	2	0	0	0	0	2
.......................................CCACTAGGAAATATGATATC.....................................................................................................	20	3	13	0.08	1	0	1	0	0	0

GAATAGTCTAGGCGTTACGAAGGGCTAGTGGTACTTAAAGGTGAGCTTTTATACTATACAAGCCGTAGGAGGCCCGTGTTACACTTCCTCCTACGGTTCGTATAATATAAAAGAACACCATCTAAGAGTCGCGTCGCGGTGGCCGACCTCCTTCGGTACC *********************************((((((((..((((((((((((((((.((((((((((...(((...)))..)))))))))).))))))))))))))))..)))).)))).*********************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M042 female body	V057 head	V111 male body	V042 embryo
.......................................GGTGATCCTTTATACTATA......................................................................................................	19	2	3	5.33	16	16	0	0	0
..........................AGTGGTACTTGAAGGTGAGC..................................................................................................................	20	1	1	1.00	1	0	0	1	0
..........................................................TTTGCCGTAGGAGGCCCGTGTT................................................................................	22	3	1	1.00	1	0	1	0	0
....................................AAGGGTGATCCTTTATACTA........................................................................................................	20	3	9	1.00	9	8	0	1	0
......TCTAGGCGTTACGAACGACAAG....................................................................................................................................	22	3	1	1.00	1	0	1	0	0
GAATAGTCTAGGCGTTACGAA...........................................................................................................................................	21	0	1	1.00	1	1	0	0	0
....................................AAGGGTGATCCTTTATACTAT.......................................................................................................	21	3	4	0.75	3	3	0	0	0
.......................................GGTGATCCTTTATACTATAG.....................................................................................................	20	3	13	0.69	9	9	0	0	0
............................................................................................................................ACATTCGCGTCGCGGTGCCC................	20	3	3	0.33	1	0	1	0	0
.............................................................................................................................AAATCGCGTCGCGGTCGCCGACC............	23	3	3	0.33	1	0	0	0	1
...TAGTCTAGAGGTTACGAA...........................................................................................................................................	18	2	4	0.25	1	0	0	0	1
.......................................GGTGATCCTTTATACTAT.......................................................................................................	18	2	7	0.14	1	1	0	0	0
....................................................................................................................................GTCGCGGGGGCCCACCTGCT........	20	3	20	0.05	1	0	1	0	0

Species	Coordinate	ID	Type	Alignment
droPer2	scaffold_0:11164049-11164208 -	dpe_109	confident	CTTATCAGATCCGCAATGCTTCCCGATCACCATGAATTTCCACTCGAAAATATGATATGTTCGGCATCCTCCGGGCACAATGTGAAGGAGGATGCCAAGCATATTATATTTTCTTGTGGTAGATTCTCAGCGCAGCGCCACCGGCTGGAGGAAGCCATGG
dp5	2:17318651-17318805 -	dps-mir-2503	Known_mirbase	CTTATCAGATCCGCAATGCTTCTCGCTCACCATGAATTCCCACTCGAAAATATGATATGCTCGGCATCCTCCGGGCAG-----GAAGGAGGATGCCGAGCATATTATATTTTCTTGTGGTAGATTCTCAGCGCAGCGCCACCGGCTGGAGGAAGCCATGG

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droPer2

CTTATCAGATCCGCAATGCTTCCCGATCACCATGAATTTCCACTCGAAAATATGATATGTTCGGCATCCTCCGGGCACAATGTGAAGGAGGATGCCAAGCATATTATATTTTCTTGTGGTAGATTCTCAGCGCAGCGCCACCGGCTGGAGGAAGCCATGG	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
..................................................TATGATATGTTCGGCATCCTC.........................................................................................	21	1	31.00	31	15	6	0	2	8	0
..................................................TATGATATGTTCGGCATCCTCC........................................................................................	22	1	31.00	31	1	9	0	6	10	5
...................................................ATGATATGTTCGGCATCCTC.........................................................................................	20	1	18.00	18	1	3	0	2	12	0
...................................................ATGATATGTTCGGCATCCTCC........................................................................................	21	1	16.00	16	0	8	0	3	5	0
..................................................TATGATATGTTCGGCATCCT..........................................................................................	20	1	6.00	6	2	1	0	1	1	1
.................................................ATATGATATGTTCGGCATCCT..........................................................................................	21	1	3.00	3	0	1	0	1	1	0
..................................................TATGATATGTTCGGCATCC...........................................................................................	19	1	3.00	3	0	1	0	0	2	0
........................................................................................AGGATGCCAAGCATATTATATT..................................................	22	1	2.00	2	0	2	0	0	0	0
........................................................................................AGGATGCCAAGCATATTATAT...................................................	21	1	2.00	2	0	1	0	1	0	0
..................................................TATGATATGTTCGGCATCCTCA........................................................................................	22	1	1.00	1	0	1	0	0	0	0
..................................................TATGATATGTTCGGCATCCTCCT.......................................................................................	23	3	1.00	3	0	0	0	0	3	0
..................................................TATGATATGTTCGGCATCCTCCA.......................................................................................	23	1	1.00	1	0	1	0	0	0	0
........................................................................................AGGATGCCAAGCATATTATA....................................................	20	1	1.00	1	0	1	0	0	0	0
.................................................ATATGATATGTTCGGCATCCTC.........................................................................................	22	1	1.00	1	1	0	0	0	0	0
..................................................................................TGAAGGAGGATGCCAAGCAT..........................................................	20	1	1.00	1	0	0	0	0	0	1
.............................................................................CAATGTGAAGGAGGATGCCAAGC............................................................	23	1	1.00	1	0	0	0	0	0	1
...................................................ATGATATGTTCGGCATCCTCCA.......................................................................................	22	1	1.00	1	0	0	0	0	1	0
............................................................................ACAATGTGAAGGAGGATGCCAAG.............................................................	23	1	1.00	1	0	1	0	0	0	0

dp5

CTTATCAGATCCGCAATGCTTCTCGCTCACCATGAATTCCCACTCGAAAATATGATATGCTCGGCATCCTCCGGGCAG-----GAAGGAGGATGCCGAGCATATTATATTTTCTTGTGGTAGATTCTCAGCGCAGCGCCACCGGCTGGAGGAAGCCATGG	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
...................................................ATGATATGCTCGGCATCCTC.........................................................................................	20	2	147.50	295	1	169	13	43	6	56	5	1	0	1
...................................................ATGATATGCTCGGCATCCTCC........................................................................................	21	2	35.00	70	0	49	1	12	1	6	0	0	1	0
........................................................................................AGGATGCCGAGCATATTATAT...................................................	21	1	34.00	34	0	16	1	4	1	10	2	0	0	0
.....................................................GATATGCTCGGCATCCTCC........................................................................................	19	2	17.50	35	0	28	0	4	0	3	0	0	0	0
...................................................ATGATATGCTCGGCATCCT..........................................................................................	19	2	11.00	22	0	15	0	0	0	6	1	0	0	0
.............................................................................G-----GAAGGAGGATGCCGAGCATA.........................................................	21	1	7.00	7	0	6	0	0	0	0	0	1	0	0
........................................................................................AGGATGCCGAGCATATTATA....................................................	20	1	7.00	7	0	3	0	1	0	1	1	0	1	0
........................................................................................AGGATGCCGAGCATATTATATT..................................................	22	1	5.00	5	0	2	0	2	0	1	0	0	0	0
.............................................................................G-----GAAGGAGGATGCCGAGCATATT.......................................................	23	1	4.00	4	0	4	0	0	0	0	0	0	0	0
......................................................ATATGCTCGGCATCCTCCGG......................................................................................	20	1	4.00	4	0	4	0	0	0	0	0	0	0	0
...................................................ATGATATGCTCGGCATCCTT.........................................................................................	20	2	4.00	8	0	4	0	1	1	2	0	0	0	0
....................................................TGATATGCTCGGCATCCTC.........................................................................................	19	2	4.00	8	1	7	0	0	0	0	0	0	0	0
.............................................................................G-----GAAGGAGGATGCCGAGCAT..........................................................	20	1	4.00	4	0	4	0	0	0	0	0	0	0	0
.......................................................TATGCTCGGCATCCTCCGG......................................................................................	19	1	4.00	4	0	4	0	0	0	0	0	0	0	0
.............................................................................G-----GAAGGAGGATGCCGAGCATT.........................................................	21	1	3.00	3	0	2	0	0	0	1	0	0	0	0
...................................................................................GAAGGAGGATGCCGAGCAT..........................................................	19	1	3.00	3	0	1	0	0	0	1	1	0	0	0
.............................................................................G-----GAAGGAGGATGCCGAGCATAT........................................................	22	1	3.00	3	0	2	0	0	0	1	0	0	0	0
.............................................................................G-----GAAGGAGGATGCCGAGCA...........................................................	19	1	3.00	3	0	2	0	0	0	1	0	0	0	0
.........................................................................................GGATGCCGAGCATATTATAT...................................................	20	1	3.00	3	0	3	0	0	0	0	0	0	0	0
...................................................ATGATATGCTCGGCATCC...........................................................................................	18	2	2.50	5	0	0	1	3	0	1	0	0	0	0
........................................................................................AGGATGCCGAGCATATTATT....................................................	20	1	2.00	2	0	0	0	1	0	0	1	0	0	0
.............................................................................G-----GAAGGAGGATGCCGAGCATATTAT.....................................................	25	1	2.00	2	0	2	0	0	0	0	0	0	0	0
........................................................................................AGGATGCCGAGCATATTATAA...................................................	21	1	2.00	2	0	0	0	0	0	2	0	0	0	0
.....................................................GATATGCTCGGCATCCTC.........................................................................................	18	2	2.00	4	0	3	0	1	0	0	0	0	0	0
.................................................ATATGATATGCTCGGCATCC...........................................................................................	20	2	1.50	3	0	2	0	0	0	0	1	0	0	0
....................................................TGATATGCTCGGCATCCTCC........................................................................................	20	2	1.50	3	0	3	0	0	0	0	0	0	0	0
..................................................TATGATATGCTCGGCATCCTC.........................................................................................	21	2	1.50	3	0	2	0	1	0	0	0	0	0	0
..................................................TATGATATGCTCGGCATCC...........................................................................................	19	2	1.50	3	0	3	0	0	0	0	0	0	0	0
.................................................ATATGATATGCTCGGCATCCTC.........................................................................................	22	2	1.50	3	0	1	2	0	0	0	0	0	0	0
.............................................................................G-----GAAGGAGGATGCCGAGC............................................................	18	1	1.00	1	0	0	0	1	0	0	0	0	0	0
..........................................CTCGAAAATATGATATGCTCG.................................................................................................	21	1	1.00	1	0	1	0	0	0	0	0	0	0	0
...................................................................................GAAGGAGGATGCCGAGCATAT........................................................	21	1	1.00	1	0	0	0	0	1	0	0	0	0	0
............................................................................AG-----GAAGGAGGATGCCGAG.............................................................	18	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.............................................................................G-----GAAGGAGGATGCCGAGCAA..........................................................	20	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.............................................................................G-----GAAGGAGGATGCCGAGCATATC.......................................................	23	1	1.00	1	0	0	0	1	0	0	0	0	0	0
..........................................................................................GATGCCGAGCATATTATATTT.................................................	21	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.....................................................GATATGCTCGGCATCCTCCGG......................................................................................	21	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.........................................ACTCGAAAATATGATATGCTCG.................................................................................................	22	1	1.00	1	0	1	0	0	0	0	0	0	0	0
...................................................ATGATATGCTCGGCATCCTCT........................................................................................	21	2	1.00	2	0	2	0	0	0	0	0	0	0	0
........................................................................................AGGATGCCGAGCATATTATATTT.................................................	23	1	1.00	1	0	1	0	0	0	0	0	0	0	0
......................................................ATATGCTCGGCATCCTCCGGGC....................................................................................	22	1	1.00	1	0	0	0	1	0	0	0	0	0	0
.....................................................GATATGCTCGGCATCCTCT........................................................................................	19	2	1.00	2	0	1	0	0	0	1	0	0	0	0
........................................................ATGCTCGGCATCCTCCGGGCAG-----GAAGGAG......................................................................	29	1	1.00	1	0	0	0	0	0	0	1	0	0	0
......................................................ATATGCTCGGCATCCTCCG.......................................................................................	19	1	1.00	1	0	1	0	0	0	0	0	0	0	0
........................................................................................AGGATGCCGAGCATATTATATC..................................................	22	1	1.00	1	0	0	0	0	0	1	0	0	0	0
.............................................................................G-----GAAGGAGGATGCCGAGCATAC........................................................	22	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.............................................................................G-----GAAGGAGGATGCCGAGCATAA........................................................	22	1	1.00	1	0	1	0	0	0	0	0	0	0	0
........................................................................................AGGATGCCGAGCATATTATATA..................................................	22	1	1.00	1	0	0	0	0	1	0	0	0	0	0
........................................................................................AGGATGCCGAGCATATTATTAA..................................................	22	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.............................................GAAAATATGATATGCTCGGCA..............................................................................................	21	1	1.00	1	0	1	0	0	0	0	0	0	0	0
...................................................................................GAAGGAGGATGCCGAGCATA.........................................................	20	1	1.00	1	0	0	0	0	0	0	1	0	0	0
..................................................TATGATATGCTCGGCATCCT..........................................................................................	20	2	1.00	2	0	1	0	0	0	1	0	0	0	0
....................................................................CTCCGGGCAG-----GAAGGAGGATGCCGAGC............................................................	27	1	1.00	1	0	0	0	0	0	0	1	0	0	0
........................................................................................AGGATGCCGAGCATATTATAAA..................................................	22	1	1.00	1	0	1	0	0	0	0	0	0	0	0
..........................................................................................GATGCCGAGCATATTATATTTT................................................	22	1	1.00	1	0	0	0	0	0	1	0	0	0	0
.....................................................GATATGCTCGGCATCCTCCGGGC....................................................................................	23	1	1.00	1	0	0	0	1	0	0	0	0	0	0
....................................................TGATATGCTCGGCATCCTCA........................................................................................	20	2	0.50	1	0	1	0	0	0	0	0	0	0	0
.....................................................GATATGCTCGGCATCCTCCC.......................................................................................	20	2	0.50	1	0	1	0	0	0	0	0	0	0	0
...................................................ATGATATGCTCGGCATCCC..........................................................................................	19	2	0.50	1	0	0	0	1	0	0	0	0	0	0
.............................................................................G-----GAAGGAGGATGCCGAGCT...........................................................	19	2	0.50	1	0	1	0	0	0	0	0	0	0	0
.................................................ATATGATATGCTCGGCATCCT..........................................................................................	21	2	0.50	1	0	1	0	0	0	0	0	0	0	0
....................................................TGATATGCTCGGCATCCTT.........................................................................................	19	2	0.50	1	0	1	0	0	0	0	0	0	0	0
................................................AATATGATATGCTCGGCATC............................................................................................	20	2	0.50	1	0	0	0	1	0	0	0	0	0	0
.....................................................................................AGGAGGATGCCGAGCATAT........................................................	19	2	0.50	1	0	0	0	1	0	0	0	0	0	0
.................................................ATATGATATGCTCGGCATC............................................................................................	19	2	0.50	1	0	1	0	0	0	0	0	0	0	0
...................................................ATGATATGCTCGGCATCCTA.........................................................................................	20	2	0.50	1	0	1	0	0	0	0	0	0	0	0
......................................................ATATGCTCGGCATCCTCCGT......................................................................................	20	2	0.50	1	0	1	0	0	0	0	0	0	0	0
..................................................TATGATATGCTCGGCATCCTG.........................................................................................	21	2	0.50	1	0	1	0	0	0	0	0	0	0	0
..................................................TATGATATGCTCGGCATCCTCC........................................................................................	22	2	0.50	1	0	0	0	1	0	0	0	0	0	0
....................................................TGATATGCTCGGCATCCTA.........................................................................................	19	2	0.50	1	0	0	0	0	0	1	0	0	0	0
...................................................ATGATATGCTCGGCATCCTCACT......................................................................................	23	3	0.33	1	0	0	0	0	0	0	0	1	0	0
......................................................ATATGCTCGGCATCCTCT........................................................................................	18	3	0.33	1	0	1	0	0	0	0	0	0	0	0
......................................................ATATGCTCGGCATCCTCC........................................................................................	18	3	0.33	1	0	1	0	0	0	0	0	0	0	0
........................................................................................AGGATGCCGAGCATATTACCA...................................................	21	4	0.25	1	0	0	1	0	0	0	0	0	0	0
.............................................................................G-----GAAGGAGGATGCCGAGTT...........................................................	19	5	0.20	1	0	0	0	0	0	0	1	0	0	0

dpe_109

scaffold_0:11164099-11164158 -

Known Ortholog

Canonical miRNA

intergenic

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-47.8	-47.5	-47.4