dpe_102

AGCAAAATACAATTTTACTCAACAATACGTTTTGAATTCACATTCTGCATTGGAAAAGCAATGGAGAATAGAATGTATTTGCATATTCTTTTGTTCCTTGCTTTTCCCAAGCGGAATGAGAGCAGTCGTCGCATGAATGAATGAATATTTTGTGGCTAAA *********************************.(((.((((((((...((((((((((.((((...(((((((......)))))))....))))))))))))))...)))))))).)))..**********************************	Read size	# Mismatch	Hit Count	Total Norm	Total	V111 male body	M042 female body	V050 head
..................................................TGGAAAAGCAATGGAGAATAGA........................................................................................	22	0	1	34.00	34	34	0	0
..................................................TGGAAAAGCAATGGAGAATAG.........................................................................................	21	0	1	30.00	30	30	0	0
....................................................GAAAAGCAATGGAGAATAGAATGT....................................................................................	24	0	1	22.00	22	22	0	0
....................................................GAAAAGCAATGGAGAATAGAATG.....................................................................................	23	0	1	7.00	7	7	0	0
....................................................GAAAAGCAATGGAGAATAGAAT......................................................................................	22	0	1	7.00	7	7	0	0
........................................................................................TTTTGTTCCTTGCTTTTCCCA...................................................	21	0	1	5.00	5	4	0	1
....................................................GAAAAGCAATGGAGAATAGA........................................................................................	20	0	1	4.00	4	4	0	0
...................................................TGAAAAGCAATGGAGAATAGA........................................................................................	21	1	1	3.00	3	3	0	0
..................................................TGGAAAAGCAATGGAGAATA..........................................................................................	20	0	1	2.00	2	2	0	0
..................................................TGGAAAAGCAATGGAGAATAGAA.......................................................................................	23	0	1	2.00	2	2	0	0
....................................................GAAAAGCAATGGAGAATAGAATGTATT.................................................................................	27	0	1	2.00	2	2	0	0
..................................................TGGAAAAGCAATGGAGAAT...........................................................................................	19	0	1	2.00	2	2	0	0
........................................................................................TTTTGTTCCTTGCTTTTCCCAA..................................................	22	0	1	2.00	2	2	0	0
.....................................................AAAAGCAATGGAGAATAGAATGT....................................................................................	23	0	2	1.50	3	3	0	0
....................................................GAAAAGCAATGGAGAATAGAA.......................................................................................	21	0	1	1.00	1	1	0	0
....................................................GAAAAGCAATGGACAATAGAATGT....................................................................................	24	1	1	1.00	1	1	0	0
.........................................................GCAATGGAGAATAGAATGT....................................................................................	19	0	2	1.00	2	2	0	0
...................................................TGAAAAGCAATGGAGAATAG.........................................................................................	20	1	1	1.00	1	1	0	0
..................................................TGGAAAAGCAATGGAGAATAGAAAA.....................................................................................	25	2	1	1.00	1	1	0	0
..................................................TGGAAAAGCAATGGAGAATAGAAA......................................................................................	24	1	1	1.00	1	0	1	0
........................................................................................TTTTGTTCCTTGCTTTTCCCC...................................................	21	1	1	1.00	1	1	0	0
.....................................................................................TTCTTTTGTTCCTTGCTTTTC......................................................	21	0	1	1.00	1	1	0	0
....................................................GAAAAGCAATGGAGAATAGAATT.....................................................................................	23	1	1	1.00	1	1	0	0
..................................................TGGAAAATCAATGGAGAATAGA........................................................................................	22	1	1	1.00	1	1	0	0
..................................................TGGAAAAGCAATGGAGAATAGT........................................................................................	22	1	1	1.00	1	1	0	0
................................................ATTGGAAAAGCAATGGAGAAA...........................................................................................	21	1	1	1.00	1	1	0	0
..................................................TGTAAAAGCAATGGAGAATAG.........................................................................................	21	1	2	0.50	1	1	0	0
....................................................CAAAAGCAATGGAGAATAGAAT......................................................................................	22	1	2	0.50	1	1	0	0
.........................................................GCAGTGGAGAATAGAATGT....................................................................................	19	1	2	0.50	1	1	0	0
.....................................................AAAAGCAATGGAGAATAGAATG.....................................................................................	22	0	2	0.50	1	1	0	0
.......................................................................................CTTTTGTTCCTTCCTTTTC......................................................	19	1	5	0.20	1	1	0	0
........................................................................................TTTTGTTCCTTGCTTTTCCTT...................................................	21	2	9	0.11	1	0	1	0

TCGTTTTATGTTAAAATGAGTTGTTATGCAAAACTTAAGTGTAAGACGTAACCTTTTCGTTACCTCTTATCTTACATAAACGTATAAGAAAACAAGGAACGAAAAGGGTTCGCCTTACTCTCGTCAGCAGCGTACTTACTTACTTATAAAACACCGATTT **********************************.(((.((((((((...((((((((((.((((...(((((((......)))))))....))))))))))))))...)))))))).)))..*********************************	Read size	# Mismatch	Hit Count	Total Norm	Total	V042 embryo
......................GTCATGAAAAACTTAAGT........................................................................................................................	18	2	11	0.09	1	1

Species	Coordinate	ID	Type	Alignment
droPer2	scaffold_10:2579165-2579324 +	dpe_102	confident	AGCAAAATACAATTTTACTCAACAATACGTTTTGAATTCACATTCTGCATTGGAAAAGCAATGGAGAATAGAATGTATTTGCATATTCTTTTGTTCCTTGCTTTTCCCAAGCGGAATGA--GAGCAGTCGTCGCATGAATGAATGAATATTTTGTGGCTAAA
dp5	4_group4:3551317-3551474 +	dps-mir-2510	Known_mirbase	AGCAAAATACAATTTTACTCAACAATACGTTTTGAATTCACATTCTGCATTGAAAAAGCAATGGATAATAGAATGTATTTGCAAATTCTATTGTTCCTTGCTTTTCCTAAGCGGGATGGGGGAGCAGTCGTCGCATGAA----TGAATATTTTTTGGCTAAA

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droPer2

AGCAAAATACAATTTTACTCAACAATACGTTTTGAATTCACATTCTGCATTGGAAAAGCAATGGAGAATAGAATGTATTTGCATATTCTTTTGTTCCTTGCTTTTCCCAAGCGGAATGA--GAGCAGTCGTCGCATGAATGAATGAATATTTTGTGGCTAAA	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
..................................................TGGAAAAGCAATGGAGAATAGA..........................................................................................	22	1	34.00	34	0	0	0	0	0	34
..................................................TGGAAAAGCAATGGAGAATAG...........................................................................................	21	1	30.00	30	0	0	0	0	0	30
....................................................GAAAAGCAATGGAGAATAGAATGT......................................................................................	24	1	22.00	22	0	0	0	0	0	22
....................................................GAAAAGCAATGGAGAATAGAATG.......................................................................................	23	1	7.00	7	0	0	0	0	0	7
....................................................GAAAAGCAATGGAGAATAGAAT........................................................................................	22	1	7.00	7	0	0	0	0	0	7
........................................................................................TTTTGTTCCTTGCTTTTCCCA.....................................................	21	1	5.00	5	0	0	0	1	0	4
....................................................GAAAAGCAATGGAGAATAGA..........................................................................................	20	1	4.00	4	0	0	0	0	0	4
..................................................TGGAAAAGCAATGGAGAATA............................................................................................	20	1	2.00	2	0	0	0	0	0	2
..................................................TGGAAAAGCAATGGAGAATAGAA.........................................................................................	23	1	2.00	2	0	0	0	0	0	2
....................................................GAAAAGCAATGGAGAATAGAATGTATT...................................................................................	27	1	2.00	2	0	0	0	0	0	2
..................................................TGGAAAAGCAATGGAGAAT.............................................................................................	19	1	2.00	2	0	0	0	0	0	2
........................................................................................TTTTGTTCCTTGCTTTTCCCAA....................................................	22	1	2.00	2	0	0	0	0	0	2
.....................................................AAAAGCAATGGAGAATAGAATGT......................................................................................	23	2	1.50	3	0	0	0	0	0	3
....................................................GAAAAGCAATGGAGAATAGAA.........................................................................................	21	1	1.00	1	0	0	0	0	0	1
.........................................................GCAATGGAGAATAGAATGT......................................................................................	19	2	1.00	2	0	0	0	0	0	2
..................................................TGGAAAAGCAATGGAGAATAGAAAA.......................................................................................	25	1	1.00	1	0	0	0	0	0	1
..................................................TGGAAAAGCAATGGAGAATAGAAA........................................................................................	24	1	1.00	1	0	1	0	0	0	0
........................................................................................TTTTGTTCCTTGCTTTTCCCC.....................................................	21	1	1.00	1	0	0	0	0	0	1
.....................................................................................TTCTTTTGTTCCTTGCTTTTC........................................................	21	1	1.00	1	0	0	0	0	0	1
....................................................GAAAAGCAATGGAGAATAGAATT.......................................................................................	23	1	1.00	1	0	0	0	0	0	1
..................................................TGGAAAAGCAATGGAGAATAGT..........................................................................................	22	1	1.00	1	0	0	0	0	0	1
................................................ATTGGAAAAGCAATGGAGAAA.............................................................................................	21	1	1.00	1	0	0	0	0	0	1
.....................................................AAAAGCAATGGAGAATAGAATG.......................................................................................	22	2	0.50	1	0	0	0	0	0	1
........................................................................................TTTTGTTCCTTGCTTTTCCTT.....................................................	21	9	0.11	1	0	1	0	0	0	0

dp5

AGCAAAATACAATTTTACTCAACAATACGTTTTGAATTCACATTCTGCATTGAAAAAGCAATGGATAATAGAATGTATTTGCAAATTCTATTGTTCCTTGCTTTTCCTAAGCGGGATGGGGGAGCAGTCGTCGCATGAA----TGAATATTTTTTGGCTAAA	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
..................................................TGAAAAAGCAATGGATAATAGA..........................................................................................	22	1	68855.00	68855	1	77	3	5	3	16	208	72	68306	164
..................................................TGAAAAAGCAATGGATAATAG...........................................................................................	21	1	21185.00	21185	8	77	0	6	2	48	377	15	20641	11
..................................................TGAAAAAGCAATGGATAATAGAA.........................................................................................	23	1	6335.00	6335	2	10	0	3	0	1	22	2	6291	4
..................................................TGAAAAAGCAATGGATAATAGAAA........................................................................................	24	1	1193.00	1193	1	3	0	0	0	0	4	0	1184	1
...................................................GAAAAAGCAATGGATAATAGA..........................................................................................	21	1	1025.00	1025	0	3	0	0	0	0	1	2	1016	3
.................................................TTGAAAAAGCAATGGATAATAG...........................................................................................	22	1	806.00	806	0	8	0	2	2	3	35	0	756	0
...................................................GAAAAAGCAATGGATAATAGAA.........................................................................................	22	1	325.00	325	0	1	0	1	0	0	0	0	323	0
.................................................TTGAAAAAGCAATGGATAATAGA..........................................................................................	23	1	253.00	253	0	1	0	0	0	0	2	0	249	1
....................................................AAAAAGCAATGGATAATAGAAT........................................................................................	22	1	249.00	249	2	0	0	0	0	0	1	0	246	0
..................................................TGAAAAAGCAATGGATAATA............................................................................................	20	1	185.00	185	0	11	0	1	0	6	21	0	145	1
..................................................TGAAAAAGCAATGGATAATAGAAAA.......................................................................................	25	1	143.00	143	0	0	0	1	0	0	2	0	140	0
..................................................TGAAAAAGCAATGGATAATAGAATG.......................................................................................	25	1	122.00	122	0	0	0	0	0	0	1	0	121	0
..................................................TGAAAAAGCAATGGATAATAGAAT........................................................................................	24	1	113.00	113	0	0	0	0	0	0	0	0	112	1
...................................................GAAAAAGCAATGGATAATAG...........................................................................................	20	1	113.00	113	0	5	0	0	0	0	0	0	107	1
...................................................GAAAAAGCAATGGATAATAGAAA........................................................................................	23	1	72.00	72	0	0	0	0	0	0	0	0	72	0
..................................................TGAAAAAGCAATGGATAATAGT..........................................................................................	22	1	63.00	63	0	0	0	0	0	0	6	0	57	0
..................................................TGAAAAAGCAATGGATAATAGAAC........................................................................................	24	1	57.00	57	0	0	0	0	0	0	0	0	57	0
..................................................TGAAAAAGCAATGGATAAT.............................................................................................	19	1	54.00	54	3	9	0	3	0	2	6	0	31	0
..................................................TGAAAAAGCAATGGATAATAGAT.........................................................................................	23	1	49.00	49	0	1	0	0	0	0	3	0	45	0
..................................................TGAAAAAGCAATGGATAATAGATC........................................................................................	24	1	44.00	44	0	0	0	0	0	0	0	0	44	0
...................................................GAAAAAGCAATGGATAATAGAAT........................................................................................	23	1	43.00	43	0	0	0	0	0	0	0	0	43	0
..................................................TGAAAAAGCAATGGATAATAA...........................................................................................	21	1	40.00	40	0	1	0	0	0	1	2	0	36	0
....................................................AAAAAGCAATGGATAATAGAA.........................................................................................	21	1	25.00	25	0	0	0	0	0	0	0	0	25	0
..................................................TGAAAAAGCAATGGATAATAGAAAAA......................................................................................	26	1	19.00	19	0	0	0	0	0	0	0	0	19	0
..................................................TGAAAAAGCAATGGATAATAGAATC.......................................................................................	25	1	15.00	15	0	0	0	0	0	0	0	0	15	0
.................................................TTGAAAAAGCAATGGATAATAGAA.........................................................................................	24	1	13.00	13	0	0	0	0	0	0	0	0	13	0
..................................................TGAAAAAGCAATGGATAATT............................................................................................	20	1	13.00	13	0	1	0	0	0	0	7	0	5	0
....................................................AAAAAGCAATGGATAATAGA..........................................................................................	20	1	13.00	13	0	0	0	0	0	0	0	0	13	0
..................................................TGAAAAAGCAATGGATAATAGAATGT......................................................................................	26	1	10.00	10	0	0	0	0	0	0	1	0	9	0
..................................................TGAAAAAGCAATGGATAATAGG..........................................................................................	22	1	10.00	10	0	1	0	0	0	0	0	0	9	0
..................................................TGAAAAAGCAATGGATAATAGAG.........................................................................................	23	1	10.00	10	0	0	0	0	0	0	0	0	10	0
........................................................................................TATTGTTCCTTGCTTTTCCTAAG...................................................	23	1	9.00	9	0	2	0	0	0	0	0	0	7	0
....................................................AAAAAGCAATGGATAATAGAATC.......................................................................................	23	1	9.00	9	0	0	0	0	0	0	0	0	9	0
..................................................TGAAAAAGCAATGGATAA..............................................................................................	18	1	7.00	7	1	0	0	0	0	0	0	0	6	0
.................................................TTGAAAAAGCAATGGATAAT.............................................................................................	20	1	7.00	7	1	1	0	0	0	0	1	0	4	0
..................................................TGAAAAAGCAATGGATAATAGTC.........................................................................................	23	1	6.00	6	0	0	0	0	0	0	3	0	3	0
..................................................TGAAAAAGCAATGGATAATCC...........................................................................................	21	1	6.00	6	0	0	0	0	0	0	2	0	4	0
..................................................TGAAAAAGCAATGGATAATAGAATGTA.....................................................................................	27	1	6.00	6	0	0	0	0	0	0	0	0	6	0
..................................................TGAAAAAGCAATGGATAATAGAAAT.......................................................................................	25	1	6.00	6	0	0	0	0	0	0	0	0	6	0
..................................................TGAAAAAGCAATGGATAATAGC..........................................................................................	22	1	6.00	6	0	0	0	1	0	0	1	0	4	0
................................................ATTGAAAAAGCAATGGATAATAG...........................................................................................	23	1	5.00	5	0	0	0	0	0	0	0	0	5	0
..................................................TGAAAAAGCAATGGATAATAGAATTC......................................................................................	26	1	5.00	5	0	0	0	0	0	0	0	0	5	0
..................................................TGAAAAAGCAATGGATAATC............................................................................................	20	1	5.00	5	0	1	0	0	0	0	0	0	4	0
..................................................TGAAAAAGCAATGGATAATAGAATA.......................................................................................	25	1	4.00	4	0	0	0	0	0	0	0	0	4	0
..................................................TGAAAAAGCAATGGATAATAGAC.........................................................................................	23	1	4.00	4	0	0	0	0	0	0	0	0	4	0
..................................................TGAAAAAGCAATGGATAAA.............................................................................................	19	1	4.00	4	0	0	0	0	0	2	0	0	2	0
..................................................TGAAAAAGCAATGGATAATAC...........................................................................................	21	1	4.00	4	0	0	0	0	0	0	1	0	3	0
..................................................TGAAAAAGCAATGGATAATAT...........................................................................................	21	1	4.00	4	0	0	0	0	0	0	1	0	3	0
..................................................TGAAAAAGCAATGGATAATAGAATT.......................................................................................	25	1	3.00	3	0	0	0	0	0	0	0	0	3	0
..................................................TGAAAAAGCAATGGATAATCAC..........................................................................................	22	1	3.00	3	0	0	0	0	0	0	0	0	3	0
.................................................TTGAAAAAGCAATGGATAATA............................................................................................	21	1	3.00	3	0	0	0	0	0	0	0	0	3	0
..........................................................................................TTGTTCCTTGCTTTTCCTAAG...................................................	21	1	3.00	3	0	0	0	0	0	0	0	0	3	0
........................................................................................TATTGTTCCTTGCTTTTCCTAA....................................................	22	1	3.00	3	0	1	0	0	0	0	0	0	2	0
...................................................GAAAAAGCAATGGATAATAGAAAA.......................................................................................	24	2	3.00	6	0	0	0	0	0	0	0	0	6	0
.....................................................AAAAGCAATGGATAATAGAAT........................................................................................	21	1	3.00	3	0	0	0	0	0	0	0	0	3	0
........................................................................................TATTGTTCCTTGCTTTTCCTA.....................................................	21	1	3.00	3	0	0	0	0	0	0	0	0	3	0
......................................................................................TCTATTGTTCCTTGCTTTTCCG......................................................	22	1	3.00	3	0	0	0	0	0	0	0	0	3	0
....................................................AAAAAGCAATGGATAATAG...........................................................................................	19	1	3.00	3	0	1	0	0	0	0	0	0	2	0
.................................................TTGAAAAAGCAATGGATAATAGAAA........................................................................................	25	1	3.00	3	0	0	0	0	0	0	0	0	3	0
..................................................TGAAAAAGCAATGGATAG..............................................................................................	18	1	3.00	3	0	0	0	0	0	0	0	0	3	0
.................................................TTGAAAAAGCAATGGATAATAGAAT........................................................................................	25	1	3.00	3	0	0	0	0	0	0	0	0	3	0
....................................................AAAAAGCAATGGATAATAGAATT.......................................................................................	23	1	3.00	3	0	0	0	0	0	0	0	0	3	0
..................................................TGAAAAAGCAATGGATAATAGATCT.......................................................................................	25	1	3.00	3	0	0	0	0	0	0	0	0	3	0
.................................................TTGAAAAAGCAATGGATAA..............................................................................................	19	1	3.00	3	0	0	0	0	0	0	0	0	3	0
........................................................................................TATTGTTCCTTGCTTTTCCTT.....................................................	21	1	2.00	2	0	0	0	0	0	0	0	0	2	0
......................................................AAAGCAATGGATAATAGA..........................................................................................	18	1	2.00	2	0	0	0	0	0	0	0	0	2	0
..................................................TGAAAAAGCAATGGATAATAGACC........................................................................................	24	1	2.00	2	0	0	0	0	0	0	0	0	2	0
.........................................................................................ATTGTTCCTTGCTTTTCCTAA....................................................	21	1	2.00	2	0	0	0	0	0	0	0	0	2	0
..................................................TGAAAAAGCAATGGA.................................................................................................	15	1	2.00	2	0	0	0	0	0	0	0	0	2	0
..................................................TGAAAAAGCAATGGATAATAGAATCC......................................................................................	26	1	2.00	2	0	0	0	0	0	0	0	0	2	0
....................................................AAAAAGCAATGGATAATAGAAA........................................................................................	22	1	2.00	2	0	0	0	0	0	0	0	0	2	0
..................................................TGAAAAAGCAATGGATA...............................................................................................	17	1	2.00	2	0	0	0	0	0	0	0	0	2	0
..................................................TGAAAAAGCAATGGATAATAGAACC.......................................................................................	25	2	2.00	4	0	0	0	0	0	0	0	0	4	0
...................................................GAAAAAGCAATGGATAATAGT..........................................................................................	21	1	2.00	2	0	1	0	0	0	0	0	0	1	0
..................................................TGAAAAAGCAATGGATAATAGAAG........................................................................................	24	1	2.00	2	0	0	0	0	0	0	0	0	2	0
...................................................GAAAAAGCAATGGATAATAGAATT.......................................................................................	24	1	2.00	2	0	0	0	0	0	0	0	0	2	0
...................................................GAAAAAGCAATGGATAAT.............................................................................................	18	1	2.00	2	0	1	0	0	0	0	0	0	1	0
..................................................TGAAAAAGCAATGGATAGA.............................................................................................	19	4	1.75	7	0	0	0	0	0	0	1	0	6	0
.........................................................GCAATGGATAATAGA..........................................................................................	15	2	1.50	3	0	0	0	0	0	0	0	0	3	0
...................................................GAAAAAGCAATGGATAATAGAAC........................................................................................	23	2	1.50	3	0	0	0	0	0	0	0	0	3	0
..................................................TGAAAAAGCAATGGATAATAGAAAAG......................................................................................	26	2	1.50	3	0	0	0	0	0	0	0	0	3	0
..................................................TGAAAAAGCAATGGATAATCCC..........................................................................................	22	3	1.33	4	0	0	0	0	0	0	2	0	2	0
...................................................GAAAAAGCAATGGATAATAA...........................................................................................	20	1	1.00	1	0	0	0	0	0	0	0	0	1	0
..................................................TGAAAAAGCAATGGATAATAGAAATG......................................................................................	26	1	1.00	1	0	0	0	0	0	0	0	0	1	0
.................................................TTGAAAAAGCAATGGATAATAGAATG.......................................................................................	26	1	1.00	1	0	0	0	0	0	0	0	0	1	0
.......................................................AAGCAATGGATAATAG...........................................................................................	16	2	1.00	2	0	0	0	0	0	0	0	0	2	0
...................................................GAAAAAGCAATGGATAATAGAATG.......................................................................................	24	1	1.00	1	0	0	0	0	0	0	0	0	1	0
.......................................................................................CTATTGTTCCTTGCTTTTCC.......................................................	20	1	1.00	1	0	0	0	1	0	0	0	0	0	0
......................................................................................TCTATTGTTCCTTGCTTTTCCC......................................................	22	1	1.00	1	0	0	0	1	0	0	0	0	0	0
.....................................................AAAAGCAATGGATAATAGA..........................................................................................	19	1	1.00	1	0	0	0	0	0	0	0	0	1	0
...................................................GAAAAAGCAATGGATAATAGAATGTA.....................................................................................	26	1	1.00	1	0	0	0	0	0	0	0	0	1	0
...........................................................................................TGTTCCTTGCTTTTCCTAAG...................................................	20	1	1.00	1	0	1	0	0	0	0	0	0	0	0
..................................................TGAAAAAGCAATGGATAATTC...........................................................................................	21	2	1.00	2	0	0	0	0	0	1	0	0	1	0
.....................................................AAAAGCAATGGATAATAG...........................................................................................	18	1	1.00	1	0	0	0	0	0	0	0	0	1	0

dpe_102

scaffold_10:2579215-2579274 +

Known Ortholog

Testes-restricted

intergenic

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-36.2	-36.0	-35.9